Liquid-based cell flaking, dyeing and sealing all-in-one machine and method

Abstract

The invention provides a liquid-based cell slide preparation, dyeing and sealing all-in-one machine and method, the all-in-one machine comprises a rack, a pretreatment system and a liquid-based cell slide preparation system, the rack comprises a first platform, a second platform and a third platform which are stacked from top to bottom, and the pretreatment system is used for pretreating a collected sample; the flaking and dyeing system is used for flaking and dyeing the pretreated sample; the sheet sealing system is used for packaging the dyed sample; the sample bottle conveying system is used for conveying sample bottles among the first platform, the second platform and the third platform; the test tube conveying system is used for conveying the test tubes between the first platform and the second platform; the control system is used for controlling the pretreatment system, the slide preparation and dyeing system, the slide sealing system, the sample bottle conveying system and the test tube conveying system; and full automation of liquid-based cell sheet preparation, dyeing and sheet sealing can be realized.

Description

Technical Field

[0001] The present invention relates to the technical field of biological sample analysis and detection, and in particular to a liquid-based cell slide preparation, staining and sealing integrated machine and method. Background Art

[0002] Exfoliative cytology utilizes normal exfoliated cells from human tissue for diagnosis. Liquid-based cytology, a subset of exfoliative cytology, involves placing traditionally difficult-to-process exfoliated cytology specimens in a liquid medium to remove interfering components such as blood and mucus, thereby improving diagnostic efficiency. Compared to traditional cervical scraping and Pap smear tests, liquid-based cytology significantly improves specimen satisfaction and the detection rate of abnormal cervical cells.

[0003] Liquid-based cytology examinations typically include slide preparation, staining, and encapsulation. Traditionally, these steps are performed manually, requiring the slide preparer to work for a long time and resulting in poor slide quality due to human interference.

[0004] There are some semi-automatic slide preparation and staining machines that can meet different usage requirements. However, their degree of automation is not high. For example, separation liquid is manually added to the centrifuge tube, the centrifuge tube containing the separation liquid, the pipette syringe, and the sample are placed in the sample processing machine for pre-processing operations, and then the centrifuge tube containing the separation liquid and the sample to be tested is manually placed in the centrifuge to complete centrifugation, and then the centrifuge tube is manually taken out of the centrifuge. Summary of the Invention

[0005] In view of the above problems existing in the prior art, the present invention provides a liquid-based cell slide preparation, staining and sealing all-in-one machine and method to solve the problems existing in the background technology.

[0006] In order to achieve the above-mentioned object of the invention, the technical solution adopted by the present invention is as follows:

[0007] Liquid-based cell preparation, staining and sealing machine, including:

[0008] A frame, comprising a first platform, a second platform and a third platform stacked from top to bottom,

[0009] A preprocessing system, used for preprocessing the collected samples;

[0010] Slide preparation and staining system, used for slide preparation and staining of pre-treated samples;

[0011] Sealing system, used to seal the stained samples;

[0012] A sample bottle conveying system, used for transferring sample bottles between the first platform, the second platform and the third platform;

[0013] A test tube transport system, used for transporting the test tube between the first platform and the second platform;

[0014] A control system for controlling the pretreatment system, slide preparation and staining system, slide sealing system, sample bottle conveying system, and test tube conveying system;

[0015] The pretreatment system and sample bottle conveying system are distributed on the first platform, the second platform and the third platform, the test tube conveying system is distributed on the first platform and the second platform, and the slide preparation and staining system and the sealing system are arranged on the second platform.

[0016] In one embodiment, the pretreatment system comprises:

[0017] The sample scanning module is used to scan the QR code on the surface of the sample bottle storing the sample and store the scanned information in the control system;

[0018] The slide coding module is used to code the information obtained from the QR code on the surface of the sample bottle by the sample scanning module on the surface of the slide placed on the fixture;

[0019] A capping module, used for opening and closing the cap on the sample bottle;

[0020] A sampling module for transferring samples into centrifuge tubes;

[0021] a centrifugation module, used for centrifuging the sample to obtain a cell solution;

[0022] A waste liquid transfer module is used to remove the upper waste liquid in the centrifuge tube;

[0023] A sample bottle recovery module is used to recover used sample bottles;

[0024] Among them, the sample scanning module and the slide coding module are arranged on the first platform, the cover opening module, the sampling module and the waste liquid transfer module are arranged on the second platform, and the centrifugation module and the sample bottle recovery module are arranged on the third platform.

[0025] In one embodiment,

[0026] The sample bottle conveying system comprises:

[0027] A first sample bottle conveying module, used for driving the sample bottle to move horizontally on the first platform;

[0028] A second sample bottle conveying module is used to drive the sample bottle to move up and down between the first platform, the second platform and the third platform;

[0029] The test tube transport system comprises:

[0030] A first test tube transport module, used for driving the test tube to move horizontally on the first platform;

[0031] a second test tube transport module, configured to drive the test tube to move up and down between the first platform and the second platform;

[0032] a third test tube transport module, configured to drive the test tube to move horizontally on the second platform;

[0033] The fourth test tube transport module is used to drive the test tube back to the first platform.

[0034] In one embodiment, the film making and dyeing system comprises:

[0035] The slide preparation module is used to pass the cell solution through the sedimentation tube to make the cell nuclei adhere to the glass slide and fix them;

[0036] A staining module for staining slides with cell nuclei adhered to them;

[0037] Air drying module, used to air dry the coverslips to prevent them from sticking together.

[0038] In one embodiment, the coverslip system comprises:

[0039] Glue dispensing module, used to add glue to the stained slides and cover them with coverslips;

[0040] The encapsulation module is used to press and encapsulate the sample with added gum and covered with a cover glass.

[0041] The present invention also provides a liquid-based cell slide preparation, staining and sealing method.

[0042] include:

[0043] S1. The preprocessing system preprocesses the collected samples;

[0044] S2. The slide preparation and staining system prepares and stains the pretreated samples;

[0045] S3. The sealing system seals the stained samples.

[0046] In one embodiment,

[0047] Step S1 includes:

[0048] S11. The sample scanning module scans the QR code on the surface of the sample bottle for storing the sample and stores the scanned information in the control system;

[0049] S12. The slide coding module uses the sample scanning module to obtain the information from the QR code on the surface of the sample bottle and codes the surface of the slide placed on the fixture;

[0050] S13. The sampling module transfers a portion of the sample to a centrifuge tube;

[0051] S14. The centrifugation module centrifuges the sample to obtain a cell solution;

[0052] S15. The waste liquid transfer module removes the upper layer of waste liquid in the centrifuge tube;

[0053] S16. The sample bottle recycling module organizes and recycles the used sample bottles.

[0054] In one embodiment,

[0055] Step S2 includes:

[0056] S21. Slide preparation module, used to pass the cell solution through a sedimentation tube to allow the cell nuclei to adhere to the glass slide and be fixed;

[0057] S22. The staining module stains the glass slide with the cell nuclei adhered thereto;

[0058] S23. The air-drying module air-dries the stained slides.

[0059] In one embodiment,

[0060] The step S3 comprises:

[0061] S31. The dispensing module adds glue to the stained and air-dried slide and covers it with a coverslip;

[0062] S32. The packaging module will press and package the sample with the added gum and the cover glass.

[0063] Compared with the existing technology, the liquid-based cell preparation, staining and sealing all-in-one machine provided by the present invention can realize full automation of liquid-based cell preparation, staining and sealing by integrating various systems into one device and implementing linkage control. No manual operation is required from the time the sample is loaded onto the machine to the time the device is loaded, thus saving manpower and material resources. At the same time, the various systems are distributed on three platforms, namely, upper, middle and lower, which greatly improves the integration rate of the equipment and effectively reduces the overall volume of the equipment and the space occupied. BRIEF DESCRIPTION OF THE DRAWINGS

[0064] Figure 1 It is a schematic diagram of a first partial three-dimensional structure of the present invention;

[0065] Figure 2 is a schematic diagram of a second partial three-dimensional structure of the present invention;

[0066] Figure 3 It is a partial three-dimensional structural diagram of the first sample bottle conveying module and the first test tube conveying module in the present invention;

[0067] Figure 4 Schematic diagram of a partial three-dimensional structure of the second test tube transport module in the present invention;

[0068] Figure 5 Schematic diagram of a partial three-dimensional structure of the third test tube transport module in the present invention;

[0069] Figure 6 Schematic diagram of a partial three-dimensional structure of the fourth test tube transport module in the present invention;

[0070] Figure 7 It is a partial three-dimensional structural diagram of the second sample bottle conveying module and the sample bottle recovery module in the present invention. DETAILED DESCRIPTION

[0071] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "axial", "radial", etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0072] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of the technical features being referred to. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, "plurality" means more than two, unless otherwise specifically defined.

[0073] In the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," "connect," "fixed," etc. should be understood broadly. For example, they may refer to fixed connection, detachable connection, or integration; mechanical connection or electrical connection; direct connection or indirect connection through an intermediate medium; internal communication between two components or interaction between two components. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on specific circumstances.

[0074] In the present invention, unless otherwise expressly specified or limited, when a first feature is "above" or "below" a second feature, it may mean that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediary. Furthermore, when a first feature is "above," "above," or "above" a second feature, it may mean that the first feature is directly above or diagonally above the second feature, or simply means that the first feature is at a higher level than the second feature. When a first feature is "below," "below," or "below" a second feature, it may mean that the first feature is directly below or diagonally below the second feature, or simply means that the first feature is at a lower level than the second feature.

[0075] In the description of this specification, the reference terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" mean that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art can combine and combine different embodiments or examples described in this specification and features of different embodiments or examples without contradiction.

[0076] like Figure 1-7 For the convenience of description, the directions of “up”, “down”, “left”, “right”, “front” and “back” in the present invention are as shown in the attached figure. Figure 1 The directions shown shall prevail;

[0077] The liquid-based cell preparation, staining and sealing machine provided by the present invention comprises a frame 1, which comprises a first platform 101, a second platform 102 and a third platform 103 stacked from top to bottom.

[0078] Preprocessing system 2, used for preprocessing the collected samples;

[0079] The slide preparation and staining system 3 is used to prepare and stain the pre-treated samples;

[0080] The sealing system 4 is used to seal the stained samples;

[0081] The sample bottle conveying system 6 is used for transporting the sample bottle 10 between the first platform 101, the second platform 102 and the third platform 103;

[0082] A test tube transport system 7, used for transporting the test tube 11 between the first platform 101 and the second platform 102;

[0083] Control system 8, used to control pretreatment system 2, slide preparation and staining system 3, sealing system 4, sample bottle conveying system 6 and test tube conveying system 7;

[0084] The pretreatment system 2 and the sample bottle conveying system 6 are distributed on the first platform 101, the second platform 102 and the third platform 103, the test tube conveying system 7 is distributed on the first platform 101 and the second platform 102, and the slide preparation and staining system 3 and the sealing system 4 are arranged on the second platform 102.

[0085] Specifically, the pre-processing system 2 includes:

[0086] The sample scanning module 201 is used to scan the QR code on the surface of the sample bottle 10 storing the sample and store the scanned information in the control system;

[0087] The slide coding module 202 is used to code the surface of the slide placed on the fixture 13 according to the information obtained from the QR code on the surface of the sample bottle 10 by the sample scanning module 201;

[0088] A cap opening module 203 is used to open and close the cap on the sample bottle 10;

[0089] A sampling module for transferring samples into centrifuge tubes;

[0090] a centrifugation module 204 for centrifuging the sample to obtain a cell solution;

[0091] A waste liquid transfer module is used to remove the upper waste liquid in the centrifuge tube;

[0092] The sample bottle recovery module 205 is used to recover the used sample bottles;

[0093] The sample scanning module 201 and the slide coding module 202 are arranged on the first platform 101, the lid opening module 203, the sampling module and the waste liquid transfer module are arranged on the second platform 102, and the centrifugation module 204 and the sample bottle recovery module 205 are arranged on the third platform 103;

[0094] Among them, the sample bottle conveying system 6 and the test tube conveying system 7 respectively utilize corresponding brackets, motors, screw rods and drive plates. The motor drives the screw rod to drive the drive plate to drive the sample bottle 10 or test tube 11 located on the bracket to move horizontally or vertically.

[0095] The sample bottle conveying system 6 includes:

[0096] The first sample bottle conveying module 601 is used to drive the frame 12 on which the sample bottles 10 are placed to move horizontally on the first platform 101;

[0097] The second sample bottle conveying module 602 is used to drive the frame 12 on which the sample bottles 10 are placed to move up and down between the first platform 101, the second platform 102 and the third platform 103.

[0098] The test tube transport system 7 comprises:

[0099] The first test tube transport module 701 is used to drive the fixture 13 on which the test tube 11 is placed to move horizontally on the first platform 101;

[0100] The second test tube transport module 702 is used to drive the fixture 13 on which the test tube 11 is placed to move up and down between the first platform 101 and the second platform 102;

[0101] The third test tube transport module 703 is used to drive the fixture 13 on which the test tube 11 is placed to move horizontally on the second platform 102;

[0102] The fourth test tube transport module 704 is used to drive the fixture 13 with the test tube 11 placed thereon back to the first platform 101 .

[0103] The slide making and dyeing system 3 includes:

[0104] The slide preparation module is used to pass the cell solution through the sedimentation tube to make the cell nuclei adhere to the glass slide and fix them;

[0105] A staining module for staining slides with cell nuclei adhered to them;

[0106] An air drying module is used to air dry the coverslips to prevent adhesion between the coverslips;

[0107] The coverslip system 4 includes:

[0108] Glue dispensing module, used to add glue to the stained slides and cover them with coverslips;

[0109] The encapsulation module is used to press and encapsulate the sample with added gum and covered with a cover glass.

[0110] The present invention also provides a liquid-based cell slide preparation, staining and sealing method.

[0111] include:

[0112] S1. The preprocessing system preprocesses the collected samples;

[0113] S2. The slide preparation and staining system prepares and stains the pretreated samples;

[0114] S3. The sealing system seals the stained samples.

[0115] Specifically,

[0116] Step S1 includes:

[0117] S11. The sample scanning module 201 scans the QR code on the surface of the sample bottle 10 for storing the sample and stores the scanned information in the control system;

[0118] S12. The slide coding module 202 uses the sample scanning module 201 to obtain the information from the QR code on the surface of the sample bottle 10 and codes the surface of the slide placed on the fixture 13;

[0119] S13. The sampling module transfers a portion of the sample to a centrifuge tube;

[0120] S14. The centrifugation module centrifuges the sample to obtain a cell solution;

[0121] S15. The waste liquid transfer module removes the upper layer of waste liquid in the centrifuge tube;

[0122] S16. The sample bottle recovery module sorts and recycles the used sample bottles;

[0123] Step S2 includes:

[0124] S21. Slide preparation module, used to pass the cell solution through a sedimentation tube to allow the cell nuclei to adhere to the glass slide and be fixed;

[0125] S22. The staining module stains the glass slide with the cell nuclei adhered thereto;

[0126] S23. The air-drying module air-dries the stained slides.

[0127] Step S3 includes:

[0128] S31. The dispensing module adds glue to the stained and air-dried slide and covers it with a coverslip;

[0129] S32. The packaging module will press and package the sample with the added gum and the cover glass.

[0130] Specifically, if Figure 5 As shown, including the following:

[0131] After the system of the all-in-one machine is initialized, several sample bottles 10 and the frames 12 for placing the sample bottles 10 are first placed on the first sample bottle conveying module 601. Preferably, four sample bottles 10 are placed in each frame 12. At the same time, several jigs 13 are placed on the first test tube conveying module 701. Four test tubes 11 of different specifications are placed on each jig 13, and the number of each test tube 11 is eight. Then, the frames 12 and the jigs 13 are correspondingly moved to the left under the drive of the first sample bottle conveying module 601 and the first test tube conveying module 701. A sample scanning module is provided above the left end of the first sample bottle conveying module 601. 201, the sample scanning module 201 scans the QR code on the surface of the sample bottle 10 moved here one by one, and stores the patient information obtained by the scan into the control system 8. At the same time, a glass slide coding module 202 is provided above the left end of the first test tube conveying module 701. The glass slide coding module 202 engraves the corresponding patient information from the sample scanning module 201 on the surface of the glass slide moved here one by one by means of coding. A second sample bottle conveying module 602 is provided at the left end of the first sample bottle conveying module 601, and a second test tube conveying module 702 is provided at the left end of the first test tube conveying module 701.

[0132] Then, each group of sample bottles 10 together with the frame 12 is driven by the second sample bottle conveying module 602 to the left side of the second platform 102. Similarly, each group of test tubes 11 together with the fixture 13 is driven by the second test tube conveying module 702 to the left side of the second platform 102 and gradually moves to the right under the drive of the third test tube conveying module 703.

[0133] Next, the cover opening module 203 provided on the second platform 102 uses the gripper contained therein to grab the sample bottle 10 located on the second sample bottle conveying module 602 in turn and open the bottle cap on the sample bottle 10. Then, the sampling module uses a pipette to suck the cell separation liquid fixed on the all-in-one machine (it is worth noting that the pipette will be automatically cleaned after each suction), and pours an appropriate amount of cell separation liquid into the centrifuge tube (the test tube 11 includes the centrifuge tube) located on the third test tube conveying module 703. The sampling module then uses the suction pipe to suck the cell separation liquid fixed on the all-in-one machine. The sample in the sample bottle is extracted by the tube and added to the centrifuge tube. After the sample in the sample bottle 10 is taken out, the opening module 203 uses the clamping claws it contains to put the bottle cap corresponding to the sample bottle 10 back on the sample bottle 10 and put it back into the frame 12. Then, the sample bottle 10 and the frame 12 are transported to the left side of the third platform 103 by the second sample bottle conveying module 602. The first push rod 6021 provided on the second sample bottle conveying module 602 pushes the sample bottle 10 together with the frame 12 to the sample bottle recovery module 205 for recovery and sorting.

[0134] The sample bottle recovery module 205 includes a sample bottle recovery box 2051. A second push rod is provided at the front end of the sample bottle recovery box 2051. The second push rod is used to push the frame 12 and the sample bottles 10 pushed into the sample bottle recovery box 2051 backward in a row to achieve the recovery and arrangement of the sample bottles 10.

[0135] The fixture 13 on which the test tube 11 is placed moves rightward to one side of the centrifugal module 204 under the drive of the second test tube transport module 702. A through hole is provided on the second platform 102 located above the centrifugal module 204. The clamping claws contained in the centrifugal module 204 clamp the centrifuge tube containing the sample to the centrifuge contained in the centrifuge module 204, and then perform an initial centrifugation operation on the centrifuge tube for a certain period of time. After the initial centrifugation is completed, the upper layer of waste liquid in the centrifuge tube is sucked out and discarded using the waste liquid transfer module; then, an appropriate amount of cleaning liquid is added to the centrifuge tube using the cleaning liquid conduit, and after the cleaning is completed, the cleaning liquid is sucked out and discarded using the waste liquid transfer module; then, cell separation liquid is poured into the centrifuge tube for a second centrifugation operation, and after the second centrifugation operation, the upper layer of waste liquid is sucked out and discarded using the waste liquid transfer module. After the second centrifugation is completed, the buffer solution fixed on the all-in-one machine is extracted using a pipette, and an appropriate amount of buffer solution is poured into the centrifuge tube to obtain a cell solution for standby use;

[0136] Before starting the slide making operation, the empty slide is also placed on the slide rack 14. First, a slide pushing device pushes the empty slide to the coding area, and the information scanned from the sample bottle by the sample scanning module 201 is printed on the corresponding slide using an inkjet device, including a QR code containing the patient information. The slide is then moved horizontally out of the clamping area, and the clamping claw places the slides one by one in the sedimentation area to wait for slide making. The clamping claw then clamps the sedimentation tube from the test tube 11 on the fixture 13 and places the sedimentation tube in the sedimentation area. The used test tube 11 and the fixture 13 are now moved to the right end of the second platform 102, and are moved to the fourth test tube conveying module 704 under the drive of the third test tube conveying module 703, and then returned to the first platform 101 under the drive of the fourth test tube conveying module 704, thereby achieving the purpose of recycling the test tube 11.

[0137] Next, use a pipette to draw an appropriate amount of the prepared cell solution into the sedimentation tube; after a certain period of time, use the waste liquid transfer module to remove the liquid in the sedimentation tube; then use the alcohol catheter to draw alcohol into the sedimentation tube for a period of time, and then remove the alcohol in the sedimentation tube again and leave it for a certain period of time to prepare for staining;

[0138] Then, use a pipette to draw the dye liquid into the sedimentation tube and keep it for a certain time. The waste liquid transfer module sucks out the dye liquid, and finally adds the fixative. Then, the upper liquid is sucked out and left for a while. Then, use the clamp to place the glass slide on the sealing system. First, the dispensing module adds neutral gum to the glass slide, and then the packaging module uses the cover glass to complete the sealing operation.

[0139] The technical features of the above-mentioned embodiments can be combined arbitrarily. In order to make the description concise, not all possible combinations of the technical features in the above-mentioned embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0140] The above-described embodiments merely illustrate several implementations of the present invention, and while their descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that a person skilled in the art would be able to make numerous variations and improvements without departing from the spirit of the present invention, all of which fall within the scope of protection of the present invention. Therefore, the scope of protection of the present invention shall be determined by the appended claims.

Claims

1. Liquid-based cell preparation, staining and sealing machine, characterized by: include: A frame, comprising a first platform, a second platform and a third platform stacked from top to bottom, A preprocessing system, used for preprocessing the collected samples; Slide preparation and staining system, used for slide preparation and staining of pre-treated samples; Sealing system, used to seal the stained samples; A sample bottle conveying system, used for transferring sample bottles between the first platform, the second platform and the third platform; A test tube transport system, used for transporting the test tube between the first platform and the second platform; A control system for controlling the pretreatment system, slide preparation and staining system, slide sealing system, sample bottle conveying system, and test tube conveying system; The pretreatment system and sample bottle conveying system are distributed on the first platform, the second platform and the third platform, the test tube conveying system is distributed on the first platform and the second platform, and the slide preparation and staining system and the sealing system are arranged on the second platform.

2. The liquid-based cell slide preparation, staining and sealing machine according to claim 1, characterized in that: The pretreatment system comprises: The sample scanning module is used to scan the QR code on the surface of the sample bottle storing the sample and store the scanned information in the control system; The slide coding module is used to code the information obtained from the QR code on the surface of the sample bottle by the sample scanning module on the surface of the slide placed on the fixture; A capping module, used for opening and closing the cap on the sample bottle; A sampling module for transferring samples into centrifuge tubes; a centrifugation module, used for centrifuging the sample to obtain a cell solution; A waste liquid transfer module is used to remove the upper waste liquid in the centrifuge tube; A sample bottle recovery module is used to recover used sample bottles; Among them, the sample scanning module and the slide coding module are arranged on the first platform, the cover opening module, the sampling module and the waste liquid transfer module are arranged on the second platform, and the centrifugation module and the sample bottle recovery module are arranged on the third platform.

3. The liquid-based cell slide preparation, staining and sealing machine according to claim 2, characterized in that: The sample bottle conveying system comprises: A first sample bottle conveying module, used for driving the sample bottle to move horizontally on the first platform; A second sample bottle conveying module is used to drive the sample bottle to move up and down between the first platform, the second platform and the third platform; The test tube transport system comprises: A first test tube transport module, used for driving the test tube to move horizontally on the first platform; a second test tube transport module, configured to drive the test tube to move up and down between the first platform and the second platform; a third test tube transport module, configured to drive the test tube to move horizontally on the second platform; The fourth test tube transport module is used to drive the test tube back to the first platform.

4. The liquid-based cell slide preparation, staining and sealing machine according to claim 3, characterized in that: The film making and dyeing system comprises: The slide preparation module is used to pass the cell solution through the sedimentation tube to make the cell nuclei adhere to the glass slide and fix them; A staining module for staining slides with cell nuclei adhered to them; Air drying module, used to air dry the coverslips to prevent them from sticking together.

5. The liquid-based cell slide preparation, staining and sealing machine according to claim 4, characterized in that: The sealing system comprises: Glue dispensing module, used to add glue to the stained slides and cover them with coverslips; The encapsulation module is used to press and encapsulate the sample with added gum and covered with a cover glass.

6. A liquid-based cell slide preparation, staining and sealing method, characterized in that: include: S1. The preprocessing system preprocesses the collected samples; S2. The slide preparation and staining system prepares and stains the pretreated samples; S3. The sealing system seals the stained samples.

7. The liquid-based cell slide preparation, staining and sealing method according to claim 6, wherein: Step S1 includes: S11. The sample scanning module scans the QR code on the surface of the sample bottle for storing the sample and stores the scanned information in the control system; S12. The slide coding module uses the sample scanning module to obtain the information from the QR code on the surface of the sample bottle and codes the surface of the slide placed on the fixture; S13. The sampling module transfers a portion of the sample to a centrifuge tube; S14. The centrifugation module centrifuges the sample to obtain a cell solution; S15. The waste liquid transfer module removes the upper layer of waste liquid in the centrifuge tube; S16. The sample bottle recycling module organizes and recycles the used sample bottles.

8. The liquid-based cell slide preparation, staining and sealing method according to claim 7, wherein: Step S2 includes: S21. Slide preparation module, used to pass the cell solution through a sedimentation tube to allow the cell nuclei to adhere to the glass slide and be fixed; S22. The staining module stains the glass slide with the cell nuclei adhered thereto; S23. The air-drying module air-dries the stained slides.

9. The liquid-based cell slide preparation, staining and sealing method according to claim 8, wherein: The step S3 comprises: S31. The dispensing module adds glue to the stained and air-dried slide and covers it with a coverslip; S32. The packaging module will press and package the sample with the added gum and the cover glass.

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