Formula and preparation method of ethanol-free pandan balsam

Abstract

The ethanol-free pandan balsam is prepared from the following raw materials in parts by weight: 10 to 20 parts of pandan leaf water extract concentrate, 15 to 25 parts of natural honey, 20 to 30 parts of coconut oil, 10 to 15 parts of shea butter, 8 to 12 parts of beewax, 1 to 4 parts of vitamin E and 0.5 to 2 parts of tea tree essential oil. The pandan leaf water extract concentrate provides fragrance and anti-inflammatory polyphenol, the natural honey has the effects of moisturizing, resisting bacteria and promoting wound healing, the coconut oil serves as a carrier matrix and can enhance skin permeability, and the shea butter has the effects of repairing skin barriers and locking water. Honey and vegetable fat are adopted to synergistically solubilize active ingredients of pandan, volatile aromatic substances and polyphenol anti-inflammatory ingredients of pandan leaves are reserved through a low-temperature process, and the pandan cream can resist depression and has the effects of aromatherapy, skin tissue repairing, moisturizing and anti-inflammation after being smeared on skin, and the cream can also be used for lip balm and balm, skin care and skin tissue repairing, anti-inflammation and the like.

Description

Technical Field

[0001] The invention relates to the technical field of ethanol-free pandan balm, in particular to a formula and a preparation method of the ethanol-free pandan balm. Background Art

[0002] Pandanus, also known as Pandan leaf or Pandanus odoratus, is a perennial evergreen shrub in the Pandanaceae family and genus Pandanus. It's known as the Oriental vanilla. Pandanus prefers to grow in a semi-sunny, sunny, humid, and hot environment, primarily in low-altitude tropical rainy regions.

[0003] Traditional ointments often contain ethanol as a solvent or preservative, which can easily lead to dry and sensitive skin. In addition, synthetic fragrances can easily cause allergies and lack skin repair effects. Therefore, there is an urgent need to develop a formula and preparation method for ethanol-free pandan ointment to solve the above technical problems. Summary of the Invention

[0004] The object of the present invention is to provide a formula and preparation method of ethanol-free pandan balm to solve the problems raised in the above background technology.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] The invention discloses a formula of ethanol-free pandan ointment, comprising the following raw materials in parts by weight: 10-20 parts of pandan leaf water extract concentrate, 15-25 parts of natural honey, 20-30 parts of coconut oil, 10-15 parts of shea butter, 8-12 parts of beeswax, 1-4 parts of vitamin E, and 0.5-2 parts of tea tree essential oil.

[0007] As a further solution of the present invention, the raw materials are as follows, in parts by weight: 14-20 parts of pandan leaf water extract concentrate, 19-25 parts of natural honey, 20-28 parts of coconut oil, 10-14 parts of shea butter, 8-11 parts of beeswax, 2-4 parts of vitamin E, and 1-2 parts of tea tree essential oil.

[0008] As a further solution of the present invention, the raw materials are as follows, in parts by weight: 16 parts of pandan leaf water extract concentrate, 22 parts of natural honey, 24 parts of coconut oil, 12 parts of shea butter, 10 parts of beeswax, 3 parts of vitamin E, and 1.5 parts of tea tree essential oil.

[0009] As a further solution of the present invention: the natural honey is Manuka honey, and its methylglyoxal content is ≥100 mg / kg.

[0010] As a further embodiment of the present invention, the acid value of the coconut oil and shea butter is ≤1.0 mg / g, and the iodine value is 7-11 g / 100 g.

[0011] As a further scheme of the present application: the beeswax is refined beeswax, the melting point is 62-65 DEG C, and the saponification value is 75-95 mg KOH / g.

[0012] A preparation method of an ethanol-free patchouli balm, comprising the following steps:

[0013] S1: patchouli extract preparation

[0014] Fresh patchouli leaves are washed and then extracted with distilled water at a ratio of 1:5 at 60 DEG C for 2 hours, and then concentrated to a solid content of ≥30% after filtration;

[0015] S2: oil phase mixing

[0016] The coconut oil, shea butter and beeswax are heated to 75 DEG C to completely melt, and then vitamin E is added and stirred uniformly;

[0017] S3: emulsification

[0018] The natural honey is preheated to 50 DEG C, then slowly added into the oil phase, and then emulsified by a homogenizer at 3000 rpm for 3 minutes;

[0019] S4: perfuming

[0020] The patchouli extract and tea tree essential oil are added when the temperature is lowered to 40 DEG C, and then stirred until the paste is uniform;

[0021] S5: filling

[0022] The hot paste is filled into sterile aluminum tubes, and then sealed after cooling.

[0023] As a further scheme of the present application: the patchouli leaf water extract is prepared by low-temperature extraction at 60 DEG C, and the content of 2-acetyl-1-pyrroline, a volatile aroma component, is ≥0.1 mg / g.

[0024] Compared with the prior art, the present application has the following beneficial effects:

[0025] The patchouli leaf water extract provides aroma and anti-inflammatory polyphenols, the natural honey has the effects of moisturizing, antibacterial and promoting wound healing, the coconut oil can enhance skin permeability as a carrier matrix, the shea butter has the effects of repairing skin barrier and water locking, the beeswax can thicken and set, vitamin E can prolong the shelf life as an antioxidant, and the tea tree essential oil and patchouli leaves can synergistically resist inflammation, the present application uses food-grade raw materials to replace ethanol, adopts honey and plant oil to synergistically solubilize patchouli active ingredients, retains volatile aromatic substances and polyphenol anti-inflammatory components of patchouli leaves through a low-temperature process, can resist depression, has aromatherapy, repairs skin tissue, is moisturizing, anti-inflammatory, and the paste can also be used for lip balm, balm, skin care, skin tissue repair, anti-inflammatory and the like. BRIEF DESCRIPTION OF DRAWINGS

[0026] Figure 1 Process flow chart for the preparation method of the non-alcohol patchouli balm. DETAILED DESCRIPTION

[0027] Various exemplary embodiments, features, and aspects of the present application will be described herein below with reference to the accompanying drawings. Like reference numerals in the drawings denote like or similar elements. Although various aspects of the embodiments are illustrated in the drawings, the drawings are not necessarily drawn to scale unless specifically indicated.

[0028] The term "exemplary" is used herein in the sense of being an example, instance, or illustration. Any embodiment described herein as "exemplary" is not necessarily to be construed as preferred or advantageous over other embodiments.

[0029] In addition, for the purpose of better illustrating the present application, numerous specific details are set forth in the following detailed description of the embodiments. Those skilled in the art will appreciate that the application can be practiced without some or all of the specific details, or with other methods, components, materials, etc. In some instances, well-known methods, components, materials, etc. have not been described in detail in order to avoid obscuring the application.

[0030] Embodiment One

[0031] A non-alcohol patchouli balm formula, comprising the following raw materials by weight: patchouli leaf water extract concentrate 10 parts, natural honey 15 parts, coconut oil 20 parts, shea butter 10 parts, beeswax 8 parts, vitamin E 1 part, tea tree essential oil 0.5 parts.

[0032] Preferably, the natural honey is Manuka honey, and the methylglyoxal content is ≥100 mg / kg.

[0033] Preferably, the acid value of the coconut oil and the shea butter is ≤1.0 mg / g, and the iodine value is 7-11 g / 100 g.

[0034] Preferably, the beeswax is refined beeswax, with a melting point of 62-65 °C and a saponification value of 75-95 mg KOH / g.

[0035] A non-alcohol patchouli balm preparation method, comprising the following steps:

[0036] S1: Preparation of patchouli extract

[0037] Fresh patchouli leaves are washed and then extracted with distilled water at a ratio of 1:5 at 60 °C for 2 hours. After filtration, the solid content is concentrated to ≥30%;

[0038] S2: Oil phase mixing

[0039] The coconut oil, shea butter, and beeswax are heated to 75 °C to completely melt, and then vitamin E is added and stirred evenly.

[0040] S3: Emulsification

[0041] The natural honey was preheated to 50℃ and then slowly added to the oil phase. The emulsification was performed in a homogenizer at 3000 rpm for 3 minutes.

[0042] S4: Perfuming

[0043] The temperature was lowered to 40℃, and the patchouli extract and tea tree oil were added. The mixture was stirred until the paste was uniform.

[0044] S5: Filling

[0045] The mixture was hot-filled into sterile aluminum tubes, which were then sealed after cooling.

[0046] Preferably, the patchouli leaf water extract is prepared by low-temperature extraction at 60℃, and the content of 2-acetyl-1-pyrroline, a volatile aroma component, is ≥0.1 mg / g.

[0047] Example Two

[0048] A formulation of an ethanol-free patchouli balm includes the following raw materials in parts by weight: patchouli leaf water extract concentrate 20 parts, natural honey 25 parts, coconut oil 30 parts, shea butter 15 parts, beeswax 12 parts, vitamin E 4 parts, and tea tree oil 2 parts.

[0049] Preferably, the natural honey is Manuka honey, and the content of methylglyoxal is ≥100 mg / kg.

[0050] Preferably, the acid value of the coconut oil and shea butter is ≤1.0 mg / g, and the iodine value is 7-11 g / 100g.

[0051] Preferably, the beeswax is refined beeswax, with a melting point of 62-65℃ and a saponification value of 75-95 mg KOH / g.

[0052] A method for preparing an ethanol-free patchouli balm includes the following steps:

[0053] S1: Preparation of patchouli extract

[0054] Fresh patchouli leaves were washed and then extracted with distilled water at a ratio of 1:5 at 60℃ for 2 hours. After filtration, the concentrate was prepared to a solid content of ≥30%.

[0055] S2: Oil phase mixing

[0056] The coconut oil, shea butter, and beeswax were heated to 75℃ to completely melt, and then vitamin E was added and stirred evenly.

[0057] S3: Emulsification

[0058] The natural honey was preheated to 50℃ and then slowly added to the oil phase. The emulsification was performed in a homogenizer at 3000 rpm for 3 minutes.

[0059] S4: Fragrance addition

[0060] When the temperature is lowered to 40℃, add the extract of piper betel and tea tree oil, and stir until the paste is uniform;

[0061] S5: Filling

[0062] Inject into sterile aluminum tubes while hot, and seal after cooling.

[0063] Preferably, the water extract of piper betel leaves is prepared by low-temperature extraction at 60℃, and the content of 2-acetyl-1-pyrroline, a volatile aroma component, is ≥0.1mg / g.

[0064] Example Three

[0065] A formula of an ethanol-free piper betel perfume paste, comprising the following raw materials in parts by weight: water extract concentrate of piper betel leaves 14 parts, natural honey 19 parts, coconut oil 20 parts, shea butter 10 parts, beeswax 8 parts, vitamin E 2 parts, tea tree oil 1 part.

[0066] Preferably, the natural honey is manuka honey, and the content of methylglyoxal is ≥100mg / kg.

[0067] Preferably, the acid value of the coconut oil and shea butter is ≤1.0mg / g, and the iodine value is 7-11g / 100g.

[0068] Preferably, the beeswax is refined beeswax, with a melting point of 62-65℃ and a saponification value of 75-95mg KOH / g.

[0069] A preparation method of an ethanol-free piper betel perfume paste, comprising the following steps:

[0070] S1: Preparation of piper betel extract

[0071] Wash fresh piper betel leaves, and extract at 60℃ for 2 hours with distilled water at a ratio of 1:5, then filter and concentrate to a solid content of ≥30%;

[0072] S2: Oil phase mixing

[0073] Heat the coconut oil, shea butter, and beeswax to 75℃ to completely melt, and add vitamin E and stir until uniform;

[0074] S3: Emulsification

[0075] Preheat the natural honey to 50℃, then slowly add the oil phase, and emulsify with a homogenizer at 3000rpm for 3 minutes;

[0076] S4: Fragrance addition

[0077] When the temperature is lowered to 40℃, add the extract of piper betel and tea tree oil, and stir until the paste is uniform;

[0078] S5: Filling

[0079] Hot injection into sterile aluminum tube, sealed after cooling.

[0080] Preferably, the water extract of Polyscias fraxiflora is prepared by low temperature extraction at 60℃, and the content of 2-acetyl-1-pyrroline, a volatile aroma component, is ≥0.1mg / g.

[0081] Example Four

[0082] A formula of Polyscias fraxiflora balm without ethanol, comprising the following raw materials in parts by weight: Polyscias fraxiflora water extract concentrate 20 parts, natural honey 25 parts, coconut oil 28 parts, shea butter 14 parts, beeswax 11 parts, vitamin E 4 parts, tea tree essential oil 2 parts.

[0083] Preferably, the natural honey is Manuka honey, and the content of methylglyoxal is ≥100mg / kg.

[0084] Preferably, the acid value of the coconut oil and shea butter is ≤1.0mg / g, and the iodine value is 7-11g / 100g.

[0085] Preferably, the beeswax is refined beeswax, with a melting point of 62-65℃ and a saponification value of 75-95mg KOH / g.

[0086] A preparation method of Polyscias fraxiflora balm without ethanol, comprising the following steps:

[0087] S1: Polyscias fraxiflora extract preparation

[0088] Fresh Polyscias fraxiflora leaves are washed and extracted with distilled water at a ratio of 1:5 at 60℃ for 2 hours, then filtered and concentrated to a solid content of ≥30%;

[0089] S2: oil phase mixing

[0090] Coconut oil, shea butter, and beeswax are heated to 75℃ to completely melt, and vitamin E is added and stirred evenly;

[0091] S3: emulsification

[0092] The natural honey is preheated to 50℃ and then slowly added to the oil phase, and emulsified in a homogenizer at 3000rpm for 3 minutes;

[0093] S4: perfuming

[0094] When the temperature is reduced to 40℃, Polyscias fraxiflora extract and tea tree essential oil are added, and the paste is stirred until uniform;

[0095] S5: filling

[0096] Hot injection into sterile aluminum tube, sealed after cooling.

[0097] Preferably, the water extract of Gynura japonica is prepared by low-temperature extraction at 60℃, and the content of 2-acetyl-1-pyrroline, a volatile aroma component, is ≥0.1mg / g.

[0098] Example Five

[0099] A formula of an ethanol-free Gynura japonica balm, comprising the following raw materials in parts by weight: Gynura japonica leaf water extract concentrate 16 parts, natural honey 22 parts, coconut oil 24 parts, shea butter 12 parts, beeswax 10 parts, vitamin E 3 parts, and tea tree essential oil 1.5 parts.

[0100] Preferably, the natural honey is Manuka honey, and the content of methylglyoxal is ≥100mg / kg.

[0101] Preferably, the acid value of the coconut oil and the shea butter is ≤1.0mg / g, and the iodine value is 7-11g / 100g.

[0102] Preferably, the beeswax is refined beeswax, with a melting point of 62-65℃ and a saponification value of 75-95mg KOH / g.

[0103] A preparation method of an ethanol-free Gynura japonica balm, comprising the following steps:

[0104] S1: Preparation of Gynura japonica extract

[0105] Fresh Gynura japonica leaves are washed and then extracted with distilled water at a ratio of 1:5 at 60℃ for 2 hours. After filtration, the extract is concentrated to a solid content of ≥30%;

[0106] S2: Mixing of oil phase

[0107] The coconut oil, shea butter, and beeswax are heated to 75℃ to completely melt, and then vitamin E is added and stirred uniformly;

[0108] S3: Emulsification

[0109] The natural honey is preheated to 50℃ and then slowly added to the oil phase. The emulsion is prepared by homogenization at 3000rpm for 3 minutes.

[0110] S4: Aromatization

[0111] The Gynura japonica extract and tea tree essential oil are added when the temperature is lowered to 40℃, and the balm is stirred until uniform.

[0112] S5: Filling

[0113] The balm is hot-filled into sterile aluminum tubes, and then sealed after cooling.

[0114] Preferably, the water extract of Gynura japonica is prepared by low-temperature extraction at 60℃, and the content of 2-acetyl-1-pyrroline, a volatile aroma component, is ≥0.1mg / g.

[0115] I. Experiment on aroma antidepressant effect

[0116] Test method: Model construction: ICR mice (n=50, half male and half female) were randomly divided into 5 groups: blank control group (no treatment), positive control group (fluoxetine 10 mg / kg), Banlangen ointment low / high dose group (odor exposure 1 h / d, equivalent to 0.5 g / 2 g ointment volatile amount), traditional ointment group (containing synthetic spices). Forced swimming test (FST) was used to evaluate depressive behavior, and the immobility time of mice within 5 minutes was recorded.

[0117] Results and analysis:

[0118] Group Immobilization time (s, Mean ± SD) p value (vs. blank group) Blank control group 185.6±22.3 - Fluoxetine group 102.4±18.7* <0.001 Low-dose patchouli balm group 148.2±20.5* 0.008 High-dose patchouli balm group 107.8±15.2* <0.001 Traditional balm group 172.3±19.1 0.312

[0119] Note: * indicates a significant difference from the blank group (one-way ANOVA, Tukey post-hoc test).

[0120] The antidepressant effect of high-dose Banlangen ointment odor exposure was comparable to that of fluoxetine (p>0.05), and significantly better than that of traditional ointment (p<0.01).

[0121] Mechanism verification: ELISA was used to detect the content of γ-aminobutyric acid (GABA) in the hippocampus of mice. The content of GABA in the Banlangen group increased by 35%, and the content of cortisol decreased by 42% (positively correlated with the behavioral results).

[0122] II. Skin repair efficacy (human clinical trial)

[0123] Test design:

[0124] Subjects: 30 healthy volunteers (20-45 years old, with dry or slightly inflamed skin) were randomly divided into Banlangen ointment group (applied twice a day) and blank matrix control group (without Banlangen extract).

[0125] Detection index:

[0126] Trans epidermal water loss (TEWL): TM300 was used to measure the inner side of the forearm;

[0127] Epidermal thickness: high-frequency ultrasound detection (SkinScanner);

[0128] Subjective score: degree of itching and desquamation (0-10 points, self-evaluated by patients).

[0129] Test results: improvement of skin barrier function

[0130] Index Patchouli balm group (change rate) Control group (change rate) P value TEWL (g / h / m 2 ) -37.2％±5.1％* -8.5％±3.2％ <0.001 Epidermal thickness (mm) +18.4％±3.7％* +2.1％±1.9％ <0.001 Pruritus score -76％±12％* -15％±8％ <0.001

[0131] Note: * indicates a significant difference between before and after within the group. ​​

[0132] Histological evidence: Skin biopsy of the P. amara group showed that the stratum corneum integrity was improved, and the expression of claudin-1 was increased by 2.3 times (immunohistochemistry).

[0133] III. Anti-inflammatory activity (in vitro cell experiment)

[0134] Experimental procedure:

[0135] Cell model: LPS-induced RAW264.7 macrophage inflammation model.

[0136] Group processing:

[0137] Blank group (not induced), model group (LPS 1 μg / ml), P. amara extract group (50 μg / ml), honey group (50 μg / ml), P. amara + honey combination group (25 μg / ml each).

[0138] Detection method:

[0139] TNF-α secretion (ELISA);

[0140] NF-κB pathway activation (Western blot detection of p65 phosphorylation level).

[0141] Data results: Inflammatory factor inhibition effect (n = 6)

[0142] Group TNF-α (pg / ml, Mean ± SD) Inhibition rate p value (vs. model group) Blank group 12.5±2.1 - Model group 485.6±35.7 - Patchouli extract group 247.8±28.4* 49％ ＜0.001 Honey group 320.5±30.2* 34％ 0.002 Patchouli + honey combination group 184.6±20.3* 62％ ＜0.001

[0143] Synergistic mechanism: The combination group significantly reduced the p65 phosphorylation level (40% lower than the P. amara group alone), confirming that honey can enhance the inhibition of polyphenols in P. amara on the NF-κB pathway.

[0144] It should be particularly noted that: the P. amara leaf water extract provides aroma and anti-inflammatory polyphenols, natural honey has the effects of moisturizing, antibacterial, and promoting wound healing, coconut oil as a carrier matrix can enhance skin permeability, shea butter has the effects of repairing skin barrier and locking water, beeswax can thicken and shape, vitamin E as an antioxidant can prolong shelf life, and tea tree essential oil and P. amara leaves synergize to have synergistic anti-inflammatory effects. The present application uses food-grade raw materials instead of ethanol, uses honey and plant oils to synergistically solubilize the active ingredients of P. amara, retains volatile aromatic substances and polyphenolic anti-inflammatory ingredients of P. amara leaves through low-temperature process, can resist depression, has aromatherapy, repairs skin tissue, moisturizes, has anti-inflammatory effects when applied to the skin, and the cream can also be used as lip balm, perfume, skin repair, anti-inflammatory, etc.

[0145] It will be obvious to a person skilled in the art that the application is not limited to the details of the above-described exemplary embodiments, but that the application can be implemented in other embodiments without departing from the scope of the application. The embodiments are therefore to be seen as exemplary and in no way restrictive, the scope of the application being defined by the claims below rather than by the above description, and all variations falling within the meaning and range of equivalency of the essential characteristics of the claims are therefore intended to be embraced therein.

[0146] Furthermore, it should be understood that although the description is made according to embodiments, not every embodiment contains only one independent technical solution, and the description is made in this way only for the sake of clarity, and a person skilled in the art should consider the description as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by a person skilled in the art.

Claims

1. A formulation of an ethanol-free patchouli balm, characterized in that, The raw materials include the following components by weight: 10-20 parts of water extract concentrate of Piper betel leaf, 15-25 parts of natural honey, 20-30 parts of coconut oil, 10-15 parts of shea butter, 8-12 parts of beeswax, 1-4 parts of vitamin E, and 0.5-2 parts of tea tree essential oil.

2. The formulation of an ethanol-free patchouli balm of claim 1, wherein, The raw materials include the following components by weight: 14-20 parts of water extract concentrate of Piper betel leaf, 19-25 parts of natural honey, 20-28 parts of coconut oil, 10-14 parts of shea butter, 8-11 parts of beeswax, 2-4 parts of vitamin E, and 1-2 parts of tea tree essential oil.

3. The formulation of an ethanol-free patchouli balm of claim 2, wherein, The raw materials include the following components by weight: 16 parts of water extract concentrate of Piper betel leaf, 22 parts of natural honey, 24 parts of coconut oil, 12 parts of shea butter, 10 parts of beeswax, 3 parts of vitamin E, and 1.5 parts of tea tree essential oil.

4. The formulation of an ethanol-free patchouli balm of claim 1, wherein, The natural honey is Manuka honey, and the content of methylglyoxal is ≥100 mg / kg.

5. The formulation of an ethanol-free patchouli balm of claim 1, wherein, The acid value of the coconut oil and the shea butter is ≤1.0 mg / g, and the iodine value is 7-11 g / 100 g.

6. The formulation of an ethanol-free patchouli balm of claim 1, wherein, The beeswax is refined beeswax, and the melting point is 62-65℃, and the saponification value is 75-95 mg KOH / g.

7. A process for the preparation of an ethanol-free patchouli balm characterized in that, The method includes the following steps: S1: Preparation of Piper betel extract Fresh Piper betel leaves are washed and then extracted with distilled water at a ratio of 1:5 at 60℃ for 2 hours. After filtration, the extract is concentrated to a solid content of ≥30%. S2: Mixing of oil phase Coconut oil, shea butter, and beeswax are heated to 75℃ to completely melt, and then vitamin E is added and stirred uniformly. S3: Emulsification The natural honey is preheated to 50℃, and then slowly added to the oil phase. The emulsion is prepared by homogenization at 3000 rpm for 3 minutes. S4: Fragrance addition The temperature is lowered to 40℃, and then the Piper betel extract and tea tree essential oil are added. The cream is stirred until uniform. S5: Filling The cream is hot-filled into sterile aluminum tubes, and then sealed after cooling.

8. A process for the preparation of an ethanol-free patchouli balm as claimed in claim 7, wherein, The water extract of Piper betel leaf is prepared by low-temperature extraction at 60℃, and the content of 2-acetyl-1-pyrroline, a volatile aroma component, is ≥0.1 mg / g.

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