A composite biological deodorant and a method for preparing the same

Abstract

The application discloses a composite biological deodorant, which comprises the following components in parts by weight: 1-3 parts of Bacillus coagulans slurry; 1-3 parts of Saccharomyces cerevisiae slurry; 1-3 parts of Lactobacillus plantarum slurry; 10-30 parts of nutrient solution A; and 20-60 parts of nutrient solution B. The composite biological deodorant is prepared by using the composite system of the Bacillus coagulans slurry, the Saccharomyces cerevisiae slurry and the Lactobacillus plantarum slurry, and combining with a specific nutrient solution carrier. The Bacillus coagulans provides environmental tolerance guarantee for the composite system due to strong environmental resistance. Metabolic products secreted by the Saccharomyces cerevisiae help to promote the proliferation of the Lactobacillus plantarum. Bacteriocins produced by the Lactobacillus plantarum selectively inhibit staphylococcus aureus, escherichia coli and other foul-smelling pathogenic bacteria, and degrade small-molecule foul-smelling substances such as ammonia nitrogen and hydrogen sulfide. The three components are synergistic, so that the deodorization and bacteriostasis effects are achieved. The product has no strong peculiar smell, and the risk of contamination by miscellaneous bacteria is effectively avoided.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of microbial preparation, in particular to a composite biological deodorant and a preparation method thereof. BACKGROUND

[0002] With the improvement of environmental protection and health requirements, biological deodorants are increasingly widely used in the fields of domestic waste treatment, sewage treatment, livestock farms and human living environment improvement due to their environmental friendliness and high safety. The existing biological deodorants are generally composed of complex microbial fermentation broth, which utilizes the mutual cooperation of each strain to decompose odor and achieve deodorization effect. However, such biological deodorants will produce a relatively strong odor after being stored for a certain period of time, which may cause secondary odor pollution and is more likely to breed miscellaneous bacteria, thereby bringing the risk of microbial pollution, especially not suitable for use in sensitive human living environments. Moreover, liquid composite deodorants also generally face the problem of low survival rate of effective bacteria. For example, the activity of non-sporulated bacteria such as plant lactobacillus and yeast bacteria decays extremely fast (the inactivation rate is often more than 90% within 3 months at room temperature), resulting in a short shelf life of the deodorant product and a significant reduction in deodorization efficiency after being stored for a period of time. Some studies have shown that solid bacterial agents prepared by freeze-drying technology can prolong the survival time of certain bacteria to some extent, but the process is costly and mainly suitable for strong-tolerance bacillus, which is difficult to economically and effectively maintain the long-term stability of a multi-strain composite system.

[0003] Chinese patent application CN202410918462.5 discloses a preparation method of a microbial deodorant. Lactic acid bacteria, bacillus and yeast are respectively subjected to liquid culture, and then mixed in proportion to form a composite bacterial liquid. The composite bacterial liquid and a carrier are uniformly mixed and dried to obtain the microbial deodorant. In this method, biochar, modified polyaspartic acid and β-cyclodextrin are used as the microbial carrier to improve the deodorization effect of the deodorant. However, the preparation process of the carrier used in this method is complex, the actual production cost is high, and the contact surface between the carrier deodorant and the odor source is limited, which slows down the absorption of malodor molecules. Compared with liquid deodorants, the application scenarios are greatly limited.

[0004] On the other hand, the current biological deodorants rely on microbial metabolic degradation of malodor substances. Microorganisms need a long time to adapt to the malodor environment before they can effectively play a role, which is slow in effect, and thus limits their application.

[0005] Therefore, for liquid biological deodorants, how to reduce the product's own odor and the risk of miscellaneous bacterial pollution, improve the deodorization efficiency and long-acting deodorization ability, and prolong the shelf life of the composite microbial system, is a key technical problem to be solved in the field. SUMMARY

[0006] In order to overcome the deficiencies of the prior art, the present application aims to provide a composite biological deodorant which can efficiently and quickly deodorize, has long-term bacteriostatic protection and high storage stability.

[0007] The present application is realized by the following technical solutions:

[0008] In a first aspect, the present application provides a composite biological deodorant comprising the following components by weight fraction:

[0009] 1-3 parts of Bacillus coagulans slurry;

[0010] 1-3 parts of Saccharomyces cerevisiae slurry;

[0011] 1-3 parts of Lactobacillus plantarum slurry;

[0012] 10-30 parts of nutrient solution A;

[0013] 20-60 parts of nutrient solution B;

[0014] The Bacillus coagulans slurry is a solid material obtained by removing supernatant after fermentation and centrifugation of Bacillus coagulans with the preservation number CICC 23843, and the viable bacterial count is 5×10 9 ~9×10 9 cfu / g;

[0015] The Saccharomyces cerevisiae slurry is a solid material obtained by removing supernatant after fermentation and centrifugation of Saccharomyces cerevisiae with the preservation number CICC 1450, and the viable bacterial count is 1×10 9 ~5×10 9 cfu / g;

[0016] The Lactobacillus plantarum slurry is a solid material obtained by removing supernatant after fermentation and centrifugation of Lactobacillus plantarum with the preservation number CICC 21791, and the viable bacterial count is 1×10 10 ~5×10 10 cfu / g;

[0017] The composition of the nutrient solution A includes: sodium acetate 12-15 g / L, ammonium sulfate 1.5-2 g / L, sodium dihydrogen phosphate 0.9-1.1 g / L, disodium hydrogen phosphate 0.9-1.1 g / L, potassium chloride 3-5 g / L, magnesium sulfate 0.08-0.12 g / L, manganese sulfate 0.04-0.05 g / L, trace element supplement 0.22-0.32 g / L; the pH of the nutrient solution A is 4.5-5;

[0018] The composition of the nutrient solution B solution comprises: glucose 15-20 g / L, sodium acetate 3-5 g / L, proteose peptone 8-12 g / L, Tween 800.8-1.2 ml / L, sodium dihydrogen phosphate 0.9-1.1 g / L, disodium hydrogen phosphate 0.9-1.1 g / L, potassium chloride 3-5 g / L, magnesium sulfate 0.08-0.12 g / L, manganese sulfate 0.04-0.05 g / L, and the pH of the nutrient solution B solution is 4.5-5.

[0019] The application provides a preparation method of the bacillus coagulans slurry, and the method comprises the following steps: inoculating bacillus coagulans into LB culture medium at an inoculation amount of 0.5-1.1%, and culturing at 28-35 DEG C and 160-180 rpm / min for 24-30 h to obtain seed liquid A; inoculating the seed liquid A into a bacillus coagulans fermentation culture medium at an inoculation amount of 2-5%, and culturing at 40-45 DEG C and 160-180 rpm / min for 30-36 h to obtain bacillus coagulans fermentation liquid; centrifuging the bacillus coagulans fermentation liquid at a rotating speed of 5000-6000 rpm / min, and discarding the supernatant to obtain the bacillus coagulans slurry; the composition of the LB culture medium comprises proteose peptone 10.0 g / L, yeast powder 5.0 g / L and NaCl 10.0 g / L; and the composition of the bacillus coagulans fermentation culture medium comprises molasses 8.0-10.0 g / L, yeast extract powder 18.0-20.0 g / L, NaCl 4.0-5.0 g / L, K2HPO4 4.0-5.0 g / L and MnSO4 8.0-10.0 mg / L.

[0020] The application provides a preparation method of the saccharomyces cerevisiae slurry, and the method comprises the following steps: inoculating saccharomyces cerevisiae into YPD culture medium at an inoculation amount of 0.5-1%, and culturing at 28-35 DEG C and 160-180 rpm / min for 24-30 h to obtain seed liquid B; inoculating the seed liquid B into YPD culture medium at an inoculation amount of 1-3%, and culturing at 25-30 DEG C and 160-180 rpm / min; when the pH reduction rate is less than 0.01, supplementing sodium acetate solution into the fermentation system to make the concentration of sodium acetate in the fermentation system reach 7-10 g / L, and continuing to ferment until the pH change rate is less than 0.01 to obtain saccharomyces cerevisiae fermentation liquid; centrifuging the saccharomyces cerevisiae fermentation liquid at a rotating speed of 3000-4000 rpm / min, and discarding the supernatant to obtain the saccharomyces cerevisiae slurry; and the composition of the YPD culture medium comprises glucose 20.0 g / L, yeast paste 10.0 g / L and proteose peptone 20.0 g / L.

[0021] The application provides a preparation method of the Lactobacillus plantarum slurry, and comprises the following steps: inoculating Lactobacillus plantarum into MRS culture medium at an inoculation amount of 3-5%, and culturing at 28-35°C for 40-48 hours to obtain seed liquid C; inoculating the seed liquid C into MRS culture medium at an inoculation amount of 5-10%, and culturing at 28-35°C for 40-48 hours to obtain Lactobacillus plantarum fermentation liquid; centrifuging the Lactobacillus plantarum fermentation liquid at a speed of 3000-3500 rpm / min, and discarding the supernatant to obtain the Lactobacillus plantarum slurry; and the MRS culture medium comprises the following components: 10.0 g / L of proteose peptone, 5.0 g / L of beef extract, 4.0 g / L of yeast extract, 2.0 g / L of triammonium citrate, 20.0 g / L of glucose, 1 ml of Tween 80, 5.0 g / L of sodium acetate, 2.0 g / L of potassium phosphate, 0.2 g / L of magnesium sulfate, 0.05 g / L of manganese sulfate, and the pH value is 6.4±0.2.

[0022] Further, the trace element supplement in the nutrient solution A liquid comprises at least one of disodium ethylenediaminetetraacetate, zinc sulfate heptahydrate, boric acid, manganese chloride tetrahydrate, cobalt chloride hexahydrate, copper sulfate pentahydrate, ammonium molybdate tetrahydrate and ferrous sulfate heptahydrate.

[0023] Further, the content of the trace element supplement in the nutrient solution A liquid is as follows: disodium ethylenediaminetetraacetate 0.05-0.075 g / L, zinc sulfate heptahydrate 0.022-0.033 g / L, boric acid 0.0114-0.171 g / L, manganese chloride tetrahydrate 0.0506-0.0759 g / L, cobalt chloride hexahydrate 0.0161-0.02415 g / L, copper sulfate pentahydrate 0.0157-0.02355 g / L, ammonium molybdate tetrahydrate 0.011-0.0165 g / L and ferrous sulfate heptahydrate 0.0499-0.0499 g / L.

[0024] Further, the nutrient solution B liquid further comprises cyclodextrin, citrus (CITRUS RETICULATA) peel extract and citric acid; the content of the cyclodextrin in the nutrient solution B liquid is 0.8-1.2 g / L, the content of the citrus (CITRUS RETICULATA) peel extract is 3-5 g / L, and the pH value of the nutrient solution is adjusted to 4.5-5 by citric acid.

[0025] The citrus (CITRUS RETICULATA) peel extract in the application can be obtained by purchase or self-preparation.

[0026] Preferably, the cyclodextrin is β-cyclodextrin.

[0027] Further, the composite biological deodorant comprises biological deodorant A liquid and biological deodorant B liquid.

[0028] The Bacillus coagulans paste, the Saccharomyces cerevisiae paste and the nutrient solution A liquid constitute a biological deodorant A liquid.

[0029] The Bacillus lactis paste and the nutrient solution B liquid constitute a biological deodorant B liquid.

[0030] Further, the biological deodorant B liquid further comprises mannitol 1.5-3wt%, inulin 0.6-1wt%, skimmed milk 0.8-1.5wt%, tea polyphenol 0.03-0.06wt% and sodium tripolyphosphate 0.02-0.05wt%.

[0031] In a second aspect, the present application provides a preparation method of the composite biological deodorant, comprising the following steps: mixing the components according to the ratio to obtain the composite biological deodorant.

[0032] The present application further provides another preparation method of the composite biological deodorant, comprising the following steps:

[0033] S1. Preparation of the biological deodorant A liquid:

[0034] According to the ratio, the Bacillus coagulans paste, the Saccharomyces cerevisiae paste and the nutrient solution A liquid are mixed to obtain the biological deodorant A liquid;

[0035] S2. Preparation of the biological deodorant B liquid:

[0036] According to the ratio, the Bacillus lactis paste and the nutrient solution B liquid are mixed to obtain the biological deodorant B liquid;

[0037] The biological deodorant A liquid and the biological deodorant B liquid are respectively stored in different containers, and the biological deodorant A liquid and the biological deodorant B liquid are mixed to obtain the composite biological deodorant when used.

[0038] In a third aspect, the present application provides a deodorizing spray comprising the composite biological deodorant.

[0039] The present application has the following beneficial effects:

[0040] 1. The present application innovatively adopts the Bacillus coagulans paste, the Saccharomyces cerevisiae paste and the Bacillus lactis paste to prepare the composite biological deodorant, which is combined with the odorless nutrient solution carrier, the Bacillus coagulans provides environmental tolerance guarantee for the composite system due to strong environmental resistance, the metabolites secreted by the Saccharomyces cerevisiae help to promote the proliferation of the Bacillus lactis, the bacteriocins (such as plantaricin) produced by the Bacillus lactis selectively inhibit Staphylococcus aureus, Escherichia coli and other foul-smelling pathogenic bacteria, and also degrade small-molecule foul-smelling substances such as ammonia nitrogen and hydrogen sulfide; the three synergistically achieve a significant deodorizing and bacteriostatic effect; the product has no strong odor, and the risk of contamination by miscellaneous bacteria is effectively avoided.

[0041] 2. The composite biological deodorizer of the present invention further incorporates citric acid, cyclodextrin, and citrus peel extract to form a composite functional adjuvant, achieving a breakthrough optimization in deodorization timeliness: the effective bacteria continuously eliminate malodorous molecules such as ammonia nitrogen and hydrogen sulfide through long-term biodegradation, while the acid neutralization ability of citric acid, the molecular inclusion characteristics of cyclodextrin, and the natural adsorption activity of citrus peel extract can achieve instantaneous capture and transformation of malodorous substances, effectively making up for the delayed start-up defect of microbial deodorization.

[0042] 3. This invention innovatively designs a graded protection strategy based on the differences in the biological characteristics of bacterial strains. By using Bacillus coagulans and Saccharomyces cerevisiae bacterial sludge, most of the Bacillus coagulans in the Bacillus coagulans sludge transforms into spores during fermentation, and most of the Saccharomyces cerevisiae in the Saccharomyces cerevisiae sludge transforms into spores during fermentation. Using an oligonutrient solution to prepare biological deodorant solution A, the survival period of Bacillus coagulans and Saccharomyces cerevisiae can be effectively extended. Meanwhile, Bacterium plantarum bacterial sludge is separately prepared using a specific protectant and nutrient solution system to prepare biological deodorant solution B, which can maintain the stability and consistency of Bacterium plantarum cells and effectively extend the survival period of Bacterium plantarum. In use, only biological deodorant solution A and biological deodorant solution B need to be mixed to activate Bacillus coagulans and Saccharomyces cerevisiae to achieve efficient deodorization. This ensures that the product maintains a stable bacterial population ratio and functional consistency throughout its entire life cycle, extending the product shelf life to 1-2 years. Detailed Implementation

[0043] The following embodiments are provided to better understand the present invention and are not limited to the preferred embodiments described. They do not constitute a limitation on the content and scope of protection of the present invention. Any product that is the same as or similar to the present invention, derived by any person under the guidance of the present invention or by combining the features of the present invention with other prior art, falls within the protection scope of the present invention.

[0044] For experiments not specifically described in the examples, the procedures or conditions should be followed according to the conventional experimental procedures described in the literature in this field. Reagents or instruments whose manufacturers are not specified are all commercially available conventional reagent products.

[0045] The materials used in the embodiments and comparative examples of the present invention are described below, but are not limited to these materials:

[0046] Bacillus coagulans: China Industrial Microbial Culture Collection Center, accession number: CICC 23843.

[0047] Saccharomyces cerevisiae: China Industrial Microbial Culture Collection Center, accession number: CICC 1450.

[0048] Lactobacillus plantarum: China General Microbiological Culture Collection Center, preservation number: CICC 21791.

[0049] LB medium: 10.0 g / L of proteose peptone, 5.0 g / L of yeast powder, 10.0 g / L of NaCl, pH = 7 ± 0.2, 121 ℃ high-pressure sterilization for 30 min.

[0050] YPD medium: 20.0 g / L of glucose, 10.0 g / L of yeast extract, 20.0 g / L of proteose peptone, 115 ℃ high-pressure sterilization for 15 min.

[0051] MRS medium: 10.0 g / L of proteose peptone, 5.0 g / L of beef extract, 4.0 g / L of yeast extract, 2.0 g / L of triammonium citrate, 20.0 g / L of glucose, 1 ml of Tween 80, 5.0 g / L of sodium acetate, 2.0 g / L of potassium phosphate dibasic, 0.2 g / L of magnesium sulfate, 0.05 g / L of manganese sulfate, pH = 6.4 ± 0.2, 121 ℃ sterilization for 30 min.

[0052] Bacillus coagulans fermentation medium: 10.0 g / L of molasses, 20.0 g / L of yeast extract powder, 5.0 g / L of NaCl, 5.0 g / L of K2HPO4, 10.0 mg / L of MnSO4, pH = 6.0-6.5, 115 ℃ high-pressure sterilization for 15 min.

[0053] Bacillus coagulans slurry: self-made, and the preparation method is as follows: Bacillus coagulans was inoculated into LB medium at an inoculation amount of 1%, and seed liquid A was obtained by culturing at 30 ℃ and 180 rpm / min for 24 h; seed liquid A was inoculated into Bacillus coagulans fermentation medium at an inoculation amount of 2%, and Bacillus coagulans fermentation liquid was obtained by culturing at 45 ℃ and 180 rpm / min for 36 h; the Bacillus coagulans fermentation liquid was centrifuged at 5000 rpm / min, and the supernatant was discarded to obtain Bacillus coagulans slurry; the viable bacterial count of the Bacillus coagulans slurry was 5 × 10 9 9 × 10 9 cfu / g.

[0054] Saccharomyces cerevisiae slurry: self-made, the preparation method is as follows: inoculate Saccharomyces cerevisiae into YPD culture medium at an inoculation amount of 1%, and culture at 30℃, 180 rpm / min for 24h to obtain seed liquid B; inoculate seed liquid B into YPD culture medium at an inoculation amount of 1%, and culture at 25℃, 180 rpm / min, when the pH reduction rate is less than 0.01, supplement sodium acetate solution to the sodium acetate concentration in the fermentation system is 8g / L at one time, continue to ferment until the pH change rate is less than 0.01, to obtain Saccharomyces cerevisiae fermentation liquor; centrifuge the Saccharomyces cerevisiae fermentation liquor at a speed of 3000 rpm / min, and discard the supernatant to obtain Saccharomyces cerevisiae slurry; the viable cell count is 1×10 9 ~5×10 9 cfu / g.

[0055] Lactobacillus plantarum slurry: self-made, the preparation method is as follows: inoculate Lactobacillus plantarum into MRS culture medium at an inoculation amount of 5%, and culture at 30℃ for 40h to obtain seed liquid C; inoculate seed liquid C into MRS culture medium at an inoculation amount of 10%, and culture at 30℃ for 40h to obtain Lactobacillus plantarum fermentation liquor; centrifuge the Lactobacillus plantarum fermentation liquor at a speed of 3500 rpm / min, and discard the supernatant to obtain Lactobacillus plantarum slurry; the viable cell count is 1×10 10 ~5×10 10 cfu / g.

[0056] Citrus peel extract solution: the content of Citrus reticulata peel extract is 10%, Guangzhou Zhongguang Biotechnology Co., Ltd.

[0057] Cyclodextrin: β-cyclodextrin, commercially available.

[0058] Examples 1-3 and Comparative Examples 1-6

[0059] A composite biological deodorant, the composition of which comprises Bacillus coagulans slurry, Saccharomyces cerevisiae slurry, Lactobacillus plantarum slurry, nutrient solution A liquid and nutrient solution B liquid;

[0060] Nutrient solution A liquid formula: sodium acetate 15g / L, ammonium sulfate 2g / L, sodium dihydrogen phosphate 1g / L, disodium hydrogen phosphate 1g / L, potassium chloride 5g / L, magnesium sulfate 0.1g / L, manganese sulfate 0.05g / L, disodium ethylenediaminetetraacetate 0.05g / L, zinc sulfate heptahydrate 0.022g / L, boric acid 0.0114g / L, manganese chloride tetrahydrate 0.0506g / L, cobalt chloride hexahydrate 0.0161g / L, copper sulfate pentahydrate 0.0157g / L, ammonium molybdate tetrahydrate 0.011g / L, ferrous sulfate heptahydrate 0.0499g / L;

[0061] Nutrient solution B liquid formula: glucose 20 g / L, sodium acetate 5 g / L, protein peptone 10 g / L, Tween 80 1 ml / L, sodium dihydrogen phosphate 1 g / L, disodium hydrogen phosphate 1 g / L, potassium chloride 5 g / L, magnesium sulfate 0.1 g / L, manganese sulfate 0.05 g / L, pH of nutrient solution 4.5-5.

[0062] According to the proportioning of Table 1, Bacillus coagulans slurry, Saccharomyces cerevisiae slurry, Lactobacillus plantarum slurry, nutrient solution A liquid and nutrient solution B liquid were mixed uniformly to prepare the composite biological deodorants of Examples 1-3 and Comparative Examples 1-6. Their deodorization and bacteriostasis effects were tested respectively.

[0063] Table 1: Compound formula of the composite biological deodorants of Examples 1-3 and Comparative Examples 1-6 (by weight fraction)

[0064]

[0065] 1. Ammonia removal rate η(NH3) and sulfur removal rate η(H2S) test: 10 ml of water (blank control group), the biological deodorants of Examples 1-3 and Comparative Examples 1-6 were inoculated into 2L closed containers containing 100g of fresh chicken manure, and each container was provided with two small beakers, one of which contained 30ml of 1mol / L dilute sulfuric acid solution, and the other contained 30ml of 1mol / L basic zinc ammonium complex solution. The containers were placed in a constant temperature incubator at 30℃ and cultured for 7d. The ammonia nitrogen content in the dilute sulfuric acid absorption solution was determined by Nash reagent spectrophotometry; the content of hydrogen sulfide in the basic zinc ammonium complex solution was determined by methylene blue spectrophotometry. Then the ammonia removal rate η(NH3) and sulfur removal rate (H2S) of each group of biological deodorants were calculated according to the following two formulas respectively:

[0066] η(NH3) = (CNH3blank-CNH3bacteria) / CNH3blank*100%

[0067] In the formula: CNH3blank is the content of ammonia in the blank group, CNH3bacteria is the content of ammonia in each deodorant group, mg / L.

[0068] η(H2S) = (CH2Sblank-CH2Sbacteria) / CH2Sblank*100%

[0069] In the formula: CH2Sblank is the content of hydrogen sulfide in the blank group, CH2Sbacteria is the content of hydrogen sulfide in each deodorant group, mg / L.

[0070] 2. Bacteriostatic test: Common odor-producing pathogenic bacteria Staphylococcus aureus and Escherichia coli were inoculated on LB plates by plate streaking method, and then cultured in a 30°C constant temperature incubator for 30h. Then, the samples were inoculated in LB medium, and cultured at 30°C and 160rpm / min for 30h to obtain Staphylococcus aureus and Escherichia coli bacterial solutions, respectively.

[0071] The Staphylococcus aureus and Escherichia coli bacterial solutions were diluted to 106CFU / mL with LB solid medium cooled to 40°C, and quickly poured into plates. After the plates were solidified, Staphylococcus aureus and Escherichia coli plates were obtained, respectively, and 10 evenly distributed marks were made on the culture dishes.

[0072] Bacteriostatic test of the tested biological deodorant on Staphylococcus aureus: Sterile Oxford cups were placed on the surface of the Staphylococcus aureus plate according to the marks, and 15μL of water, biological deodorants of Examples 1-3 and Comparative Examples 1-6 were added respectively. After drying, the Oxford cups were removed, the plates were placed in a 30°C constant temperature incubator for 24h, and the diameter of the bacteriostatic circle was observed and measured.

[0073] Bacteriostatic test of the tested biological deodorant on Escherichia coli: Sterile Oxford cups were placed on the surface of the Escherichia coli plate according to the marks, and 15μL of water, biological deodorants of Examples 1-3 and Comparative Examples 1-6 were added respectively. After drying, the Oxford cups were removed, the plates were placed in a 30°C constant temperature incubator for 24h, and the diameter of the bacteriostatic circle was observed and measured.

[0074] Table 2: Deodorization and bacteriostasis effects of the composite biological deodorants of Examples 1-3 and Comparative Examples 1-6

[0075]

[0076] As can be seen from the comparison of Examples 1-3 and Comparative Examples 1-6, the composite biological deodorant of the present application, which is compounded by Bacillus coagulans slurry, Saccharomyces cerevisiae slurry and Lactobacillus plantarum slurry, can effectively remove ammonia nitrogen and hydrogen sulfide, and has excellent bacteriostatic effect on common odor-producing pathogenic bacteria Staphylococcus aureus and Escherichia coli. The removal effect of ammonia nitrogen and hydrogen sulfide and the bacteriostatic effect on Staphylococcus aureus and Escherichia coli of Comparative Examples 1-6 using one or two of the slurry alone are not good.

[0077] Examples 4 and Comparative Examples 7-13

[0078] A composite biological deodorant, which comprises Bacillus coagulans slurry, Saccharomyces cerevisiae slurry, Lactobacillus plantarum slurry, nutrient solution A and nutrient solution B;

[0079] Nutrient solution A liquid formula: sodium acetate 15 g / L, ammonium sulfate 2 g / L, sodium dihydrogen phosphate 1 g / L, disodium hydrogen phosphate 1 g / L, potassium chloride 5 g / L, magnesium sulfate 0.1 g / L, manganese sulfate 0.05 g / L, ethylenediaminetetraacetic acid disodium 0.05 g / L, zinc sulfate heptahydrate 0.022 g / L, boric acid 0.0114 g / L, manganese chloride tetrahydrate 0.0506 g / L, cobalt chloride hexahydrate 0.0161 g / L, copper sulfate pentahydrate 0.0157 g / L, ammonium molybdate tetrahydrate 0.011 g / L, ferrous sulfate heptahydrate 0.0499 g / L.

[0080] Nutrient solution B liquid formula I: glucose 20 g, sodium acetate 5 g / L, protein peptone 10 g / L, Tween 80 1 ml / L, sodium dihydrogen phosphate 1 g / L, disodium hydrogen phosphate 1 g / L, potassium chloride 5 g / L, magnesium sulfate 0.1 g / L, manganese sulfate 0.05 g / L, cyclodextrin 1 g / L, citrus peel extract solution 32 ml / L, and the pH of the nutrient solution is adjusted to 4.5-5 with citric acid.

[0081] Nutrient solution B liquid formula II: glucose 20 g, sodium acetate 5 g / L, protein peptone 10 g / L, Tween 80 1 ml / L, sodium dihydrogen phosphate 1 g / L, disodium hydrogen phosphate 1 g / L, potassium chloride 5 g / L, magnesium sulfate 0.1 g / L, manganese sulfate 0.05 g / L, cyclodextrin 2 g / L, citrus peel extract solution 40 ml / L, and the pH of the nutrient solution is adjusted to 4.5-5 with dilute hydrochloric acid.

[0082] Nutrient solution B liquid formula III: glucose 20 g, sodium acetate 5 g / L, protein peptone 10 g / L, Tween 80 1 ml / L, sodium dihydrogen phosphate 1 g / L, disodium hydrogen phosphate 1 g / L, potassium chloride 5 g / L, magnesium sulfate 0.1 g / L, manganese sulfate 0.05 g / L, citrus peel extract solution 40 ml / L, and the pH of the nutrient solution is adjusted to 4.5-5 with citric acid.

[0083] Nutrient solution B liquid formula IV: glucose 20 g, sodium acetate 5 g / L, protein peptone 10 g / L, Tween 80 1 ml / L, sodium dihydrogen phosphate 1 g / L, disodium hydrogen phosphate 1 g / L, potassium chloride 5 g / L, magnesium sulfate 0.1 g / L, manganese sulfate 0.05 g / L, cyclodextrin 2 g / L, and the pH of the nutrient solution is adjusted to 4.5-5 with citric acid.

[0084] Nutrient solution B liquid formula V: glucose 20 g, sodium acetate 5 g / L, protein peptone 10 g / L, Tween 80 1 ml / L, sodium dihydrogen phosphate 1 g / L, disodium hydrogen phosphate 1 g / L, potassium chloride 5 g / L, magnesium sulfate 0.1 g / L, manganese sulfate 0.05 g / L, and the pH of the nutrient solution is adjusted to 3.5-4 with citric acid.

[0085] Nutrient solution B liquid formula VI: glucose 20 g, sodium acetate 5 g / L, proteose peptone 10 g / L, Tween 80 1 ml / L, sodium dihydrogen phosphate 1 g / L, disodium hydrogen phosphate 1 g / L, potassium chloride 5 g / L, magnesium sulfate 0.1 g / L, manganese sulfate 0.05 g / L, cyclodextrin 3 g / L, and the pH of the nutrient solution is adjusted to 4.5-5 with dilute hydrochloric acid.

[0086] Nutrient solution B liquid formula VII: glucose 20 g, sodium acetate 5 g / L, proteose peptone 10 g / L, Tween 80 1 ml / L, sodium dihydrogen phosphate 1 g / L, disodium hydrogen phosphate 1 g / L, potassium chloride 5 g / L, magnesium sulfate 0.1 g / L, manganese sulfate 0.05 g / L, and citrus peel extract solution 50 ml / L, and the pH of the nutrient solution is adjusted to 4.5-5 with dilute hydrochloric acid.

[0087] Nutrient solution B liquid formula VIII: glucose 20 g, sodium acetate 5 g / L, proteose peptone 10 g / L, Tween 80 1 ml / L, sodium dihydrogen phosphate 1 g / L, disodium hydrogen phosphate 1 g / L, potassium chloride 5 g / L, magnesium sulfate 0.1 g / L, manganese sulfate 0.05 g / L, cyclodextrin 3 g / L, and citrus peel extract solution 50 ml / L, and the pH of the nutrient solution is adjusted to 3.5 with citric acid.

[0088] According to the proportions in Table 3, Bacillus coagulans slurry, Saccharomyces cerevisiae slurry, Lactobacillus plantarum slurry, and nutrient solution were mixed uniformly to prepare the composite biological deodorant of Example 4 and Comparative Examples 7-13. The deodorizing effect was tested respectively.

[0089] Table 3: Compound formula of the composite biological deodorant of Example 4 and Comparative Examples 7-13 (by weight fraction)

[0090]

[0091] Deodorizing effect test: 9 groups of 50 mL of kitchen waste leachate were added to 2 L containers. The composite biological deodorants of Example 4 and Comparative Examples 7-13 were diluted 30 times and placed in a spray bottle. Eight groups of containers were sprayed with an equal amount of diluted deodorant, and the remaining one group of containers was sprayed with an equal amount of water as a blank control group. The odor degree values of the containers were detected at 1 min, 5 min, 15 min, 30 min, 1 h, and 24 h after spraying.

[0092] Table 4: Odor degree values of the composite biological deodorants of Example 4 and Comparative Examples 7-13

[0093]

[0094] As can be seen from the comparison of Example 4 and Comparative Examples 7-13, the composite biological deodorant of the present application, further introducing citric acid, cyclodextrin and CITRUS RETICULATA peel extract to form a composite functional additive, effectively makes up for the defect of delayed microbial deodorization start, and has a significant deodorizing effect 1 min after spraying and long-term deodorizing effect.

[0095] Example 5

[0096] A composite biological deodorant, which comprises Bacillus coagulans slurry, Saccharomyces cerevisiae slurry, Lactobacillus plantarum slurry, nutrient solution A and nutrient solution B;

[0097] The nutrient solution A formula is: sodium acetate 15 g / L, ammonium sulfate 2 g / L, sodium dihydrogen phosphate 1 g / L, disodium hydrogen phosphate 1 g / L, potassium chloride 5 g / L, magnesium sulfate 0.1 g / L, manganese sulfate 0.05 g / L, disodium ethylenediaminetetraacetate 0.05 g / L, zinc sulfate heptahydrate 0.022 g / L, boric acid 0.0114 g / L, manganese chloride tetrahydrate 0.0506 g / L, cobalt chloride hexahydrate 0.0161 g / L, copper sulfate pentahydrate 0.0157 g / L, ammonium molybdate tetrahydrate 0.011 g / L, ferrous sulfate heptahydrate 0.0499 g / L.

[0098] The nutrient solution B formula is: glucose 20 g / L, sodium acetate 5 g / L, protein peptone 10 g / L, Tween 80 1 ml / L, sodium dihydrogen phosphate 1 g / L, disodium hydrogen phosphate 1 g / L, potassium chloride 5 g / L, magnesium sulfate 0.1 g / L, manganese sulfate 0.05 g / L, and the nutrient solution pH is 4.5-5.

[0099] According to weight parts, 1 part of Bacillus coagulans slurry, 1 part of Saccharomyces cerevisiae slurry, 1 part of Lactobacillus plantarum slurry, 10 parts of nutrient solution A and 20 parts of nutrient solution B are uniformly mixed to prepare the composite biological deodorant of Example 5.

[0100] Example 6

[0101] A composite biological deodorant, which comprises Bacillus coagulans slurry, Saccharomyces cerevisiae slurry, Lactobacillus plantarum slurry, nutrient solution A and nutrient solution B;

[0102] Nutrient solution A liquid formula: sodium acetate 15 g / L, ammonium sulfate 2 g / L, sodium dihydrogen phosphate 1 g / L, disodium hydrogen phosphate 1 g / L, potassium chloride 5 g / L, magnesium sulfate 0.1 g / L, manganese sulfate 0.05 g / L, disodium ethylenediaminetetraacetate 0.05 g / L, zinc sulfate heptahydrate 0.022 g / L, boric acid 0.0114 g / L, manganese chloride tetrahydrate 0.0506 g / L, cobalt chloride hexahydrate 0.0161 g / L, copper sulfate pentahydrate 0.0157 g / L, ammonium molybdate tetrahydrate 0.011 g / L, ferrous sulfate heptahydrate 0.0499 g / L, pH = 4.5-5;

[0103] Nutrient solution B liquid formula: glucose 20 g, sodium acetate 5 g / L, protein peptone 10 g / L, Tween 80 1 ml / L, sodium dihydrogen phosphate 1 g / L, disodium hydrogen phosphate 1 g / L, potassium chloride 5 g / L, magnesium sulfate 0.1 g / L, manganese sulfate 0.05 g / L, cyclodextrin 1 g / L, citrus peel extract solution 32 ml / L, adjust the pH of the nutrient solution to 4.5-5 with citric acid;

[0104] Protective agent: mannitol, inulin, skim milk, tea polyphenol, sodium tripolyphosphate;

[0105] 1 part of Bacillus coagulans slurry, 1 part of Saccharomyces cerevisiae slurry, 1 part of Lactobacillus plantarum slurry, 10 parts of nutrient solution A, 20 parts of nutrient solution B were mixed, and then protective agents (final concentration of mannitol 2 wt%, inulin 0.6 wt%, skim milk 1 wt%, tea polyphenol 0.05 wt%, sodium tripolyphosphate 0.05 wt%) were added to prepare the composite biological deodorant of Example 6.

[0106] Comparative Example 14

[0107] A composite biological deodorant, which comprises Bacillus coagulans fermentation broth, Saccharomyces cerevisiae fermentation broth, and Lactobacillus plantarum fermentation broth.

[0108] Preparation of Bacillus coagulans fermentation broth: Bacillus coagulans was inoculated into LB medium at an inoculation amount of 1%, and cultured at 30°C and 180 rpm / min for 24 h to obtain seed solution A; seed solution A was inoculated into Bacillus coagulans fermentation medium at an inoculation amount of 2%, and cultured at 45°C and 180 rpm / min for 36 h to obtain Bacillus coagulans fermentation broth.

[0109] Preparation of Saccharomyces cerevisiae fermentation liquor: inoculate Saccharomyces cerevisiae into YPD culture medium at an inoculation amount of 1%, and culture at 30°C and 180 rpm / min for 24 h to obtain seed liquid B; inoculate seed liquid B into YPD culture medium at an inoculation amount of 1%, and culture at 30°C and 180 rpm / min; when the pH decrease rate is less than 0.01, add sodium acetate solution to the fermentation system to make the concentration of sodium acetate in the fermentation system 8 g / L at one time, and continue to ferment until the pH change rate is less than 0.01, to obtain Saccharomyces cerevisiae fermentation liquor.

[0110] Preparation of Lactobacillus plantarum fermentation liquor: inoculate Lactobacillus plantarum into MRS culture medium at an inoculation amount of 5%, and culture at 30°C for 40 h to obtain seed liquid C; inoculate seed liquid C into MRS culture medium at an inoculation amount of 10%, and culture at 30°C for 40 h to obtain Lactobacillus plantarum fermentation liquor.

[0111] Mix 1 part of Bacillus coagulans fermentation liquor, 1 part of Saccharomyces cerevisiae fermentation liquor and 1 part of Lactobacillus plantarum fermentation liquor by weight to obtain a composite biological deodorant of Comparative Example 14.

[0112] After opening, place Example 5-6 and Comparative Example 14 in a dark and cool place, and observe the swelling, bacterial contamination and odor value changes of the composite biological deodorant every quarter.

[0113] Table 5: Changes of the composite biological deodorants of Example 5-6 and Comparative Example 14 during storage

[0114]

[0115] As can be seen from the comparison of Example 5-6 and Comparative Example 14, the composite biological deodorant of the application, which is compounded with Bacillus coagulans slurry, Saccharomyces cerevisiae slurry, Lactobacillus plantarum slurry and a specific nutrient liquid system, can effectively improve the storage stability of the product and effectively avoid the risk of bacterial contamination, and does not produce strong odor during long-term storage.

[0116] Example 7

[0117] A composite biological deodorant comprises biological deodorant A liquid and biological deodorant B liquid; Bacillus coagulans slurry, Saccharomyces cerevisiae slurry and nutrient liquid A liquid constitute the biological deodorant A liquid; Lactobacillus plantarum slurry, nutrient liquid B liquid and protective agent (mannitol, inulin, skim milk, tea polyphenol and sodium tripolyphosphate) constitute the biological deodorant B liquid.

[0118] Nutrient solution A liquid formula: sodium acetate 15 g / L, ammonium sulfate 2 g / L, sodium dihydrogen phosphate 1 g / L, disodium hydrogen phosphate 1 g / L, potassium chloride 5 g / L, magnesium sulfate 0.1 g / L, manganese sulfate 0.05 g / L, disodium ethylenediaminetetraacetate 0.05 g / L, zinc sulfate heptahydrate 0.022 g / L, boric acid 0.0114 g / L, manganese chloride tetrahydrate 0.0506 g / L, cobalt chloride hexahydrate 0.0161 g / L, copper sulfate pentahydrate 0.0157 g / L, ammonium molybdate tetrahydrate 0.011 g / L, ferrous sulfate heptahydrate 0.0499 g / L;

[0119] Nutrient solution B liquid formula: glucose 20 g, sodium acetate 5 g / L, proteose peptone 10 g / L, Tween 80 1 ml / L, sodium dihydrogen phosphate 1 g / L, disodium hydrogen phosphate 1 g / L, potassium chloride 5 g / L, magnesium sulfate 0.1 g / L, manganese sulfate 0.05 g / L, cyclodextrin 1 g / L, citrus peel extract solution 32 ml / L, adjust the pH of the nutrient solution to 4.5-5 with citric acid;

[0120] Prepare biological deodorant A liquid: mix 1 part of Bacillus coagulans slurry, 1 part of Saccharomyces cerevisiae slurry, and 10 parts of nutrient solution A liquid to obtain biological deodorant A liquid;

[0121] Prepare biological deodorant B liquid: mix 1 part of Lactobacillus plantarum slurry and 20 parts of nutrient solution B liquid, then add protective agents (final concentration of mannitol 2 wt%, inulin 0.6 wt%, skim milk 1 wt%, tea polyphenol 0.05 wt%, sodium tripolyphosphate 0.05 wt%) to obtain biological deodorant B liquid.

[0122] Storage and use of the composite biological deodorant: store biological deodorant A liquid and biological deodorant B liquid in different containers and store them in an environment with light shielding and temperature below 25°C. When using the deodorant, pour biological deodorant A liquid into biological deodorant B liquid, mix well and stand for 5-6 h before use.

[0123] Comparative Example 15

[0124] The difference between Comparative Example 15 and Example 7 is only in the preparation of Bacillus coagulans slurry: inoculate Bacillus coagulans into LB medium at an inoculation amount of 1%, culture at 30°C and 160 rpm / min for 24 h to obtain seed solution A; inoculate seed solution A into LB medium at an inoculation amount of 2%, culture at 30°C and 180 rpm / min for 36 h to obtain Bacillus coagulans fermentation liquid, centrifuge at a speed of 5000 rpm / min, discard the supernatant, and obtain Bacillus coagulans slurry.

[0125] Comparative Example 16

[0126] The difference between Comparative Example 16 and Example 7 is only in the storage method of the composite biological deodorant: Comparative Example 16 directly mixes the biological deodorant A liquid and the biological deodorant B liquid and stores them in a light-proof environment at a temperature below 25°C; the rest is the same.

[0127] Comparative Example 17

[0128] The difference between Comparative Example 17 and Example 7 is only in the preparation of the S. cerevisiae slurry: Comparative Example 17 inoculates S. cerevisiae into YPD culture medium at an inoculation amount of 1%, and cultures it at 30°C and 180 rpm / min for 24 h to obtain seed liquid B; inoculates seed liquid B into YPD culture medium at an inoculation amount of 2%, and cultures it at 25°C and 180 rpm / min; when the pH decrease rate is less than 0.01, adds glucose solution to the fermentation system to make the glucose concentration 8 g / L at one time, and continues to ferment until the pH decrease rate is less than 0.01, to obtain S. cerevisiae fermentation liquid, which is centrifuged at a speed of 3500 rpm / min to obtain S. cerevisiae slurry.

[0129] Take the biological deodorant A liquid of Example 7, Comparative Example 15 and Comparative Example 17, and the biological deodorant of Comparative Example 16, and take samples every other month after opening to plate on LB plates and PDA plates to measure the effective viable cell count of B. coagulans and S. cerevisiae; take the biological deodorant B liquid of Example 7, Comparative Example 15 and Comparative Example 17, and the biological deodorant of Comparative Example 16, and take samples every other month after opening to plate on MRS plates to measure the effective viable cell count of L. plantarum. Record the shelf life of the bacteria when the effective viable cell count of B. coagulans, S. cerevisiae and L. plantarum decreases to 10%, and select the shortest shelf life of B. coagulans, S. cerevisiae and L. plantarum in the deodorant as the total shelf life of the deodorant (i.e. to ensure that the effective viable cell count of each strain in the composite biological deodorant is above 10%).

[0130] Table 6: Shelf life of the deodorant of Example 7 and Comparative Examples 15-17

[0131]

[0132] Take the unopened composite biological deodorant of Example 7 stored for 1 month, 6 months, 12 months, 18 months, 24 months and 25 months, open and mix the activated biological deodorant after opening, and test its ammonia removal rate, sulfur removal rate, bacteriostatic property and deodorizing effect, and take an equal amount of water as a blank control group (the first three indicators are tested according to Examples 1-3 and Comparative Examples 1-6; the last indicator is tested according to Examples 4 and Comparative Examples 7-13).

[0133] Table 7: Deodorizing and bacteriostatic effect of the composite biological deodorant of Example 7 with different storage times

[0134]

[0135] Table 8: Odor values of the composite biological deodorant of Example 7 at different storage times

[0136]

[0137] As can be seen from the comparison of Example 7 and Comparative Examples 15-17, the composite biological deodorant of the present application uses the Bacillus coagulans slurry and the Saccharomyces cerevisiae slurry to prepare the biological deodorant A liquid using the oligotrophic liquid, and uses the Lactobacillus plantarum slurry to prepare the biological deodorant B liquid using the specific protective agent and the nutrient liquid system. The biological deodorant A liquid and the biological deodorant B liquid are stored separately before use, and the shelf life of the product can be extended to 1-2 years.

[0138] Example 8

[0139] Preparation of the biological deodorant B liquid: 1 part of the Lactobacillus plantarum slurry and 20 parts of the nutrient liquid B were mixed, and then the protective agent (final concentration of 2 wt% mannitol, 0.6 wt% inulin, 1 wt% skim milk, 0.05 wt% tea polyphenol, and 0.05 wt% sodium tripolyphosphate) was added to obtain the biological deodorant B liquid.

[0140] Nutrient liquid B formula: glucose 20 g, sodium acetate 5 g / L, proteose peptone 10 g / L, Tween 80 1 ml / L, sodium dihydrogen phosphate 1 g / L, disodium hydrogen phosphate 1 g / L, potassium chloride 5 g / L, magnesium sulfate 0.1 g / L, manganese sulfate 0.05 g / L, cyclodextrin 1 g / L, citrus peel extract solution 32 ml / L, and the pH of the nutrient liquid was adjusted to 4.5-5 with citric acid.

[0141] Comparative Examples 18-27

[0142] The difference between Comparative Examples 18-27 and Example 8 is only in the formula of the protective agent added, and the specific formula of each group of protective agents is shown in Table 7.

[0143] Table 9: Formula of the protective agent added in Example 8 and Comparative Examples 18-27

[0144]

[0145] The bacterial liquid of Example 8 and Comparative Examples 18-27 was taken every other month and plated on MRS plates to measure the number of viable bacteria until the number of viable Lactobacillus plantarum bacteria decreased to less than 10%, and the length of time was recorded as the shelf life of the biological deodorant.

[0146] Table 8: Shelf life of the biological deodorant B liquid of Example 8 and Comparative Examples 18-27

[0147]

[0148] As can be seen from the comparison of Example 8 and Comparative Examples 18-27, among various protective agents, the sugar and glycolipid have the best protective effect on the Lactobacillus plantarum, and are likely to protect the biofilm of the Lactobacillus plantarum; for cost consideration, the nutrient solution is added with sufficient glucose, and the protective agent is added with 2% mannitol and 0.6% inulin; the protective effect of skimmed milk on the Lactobacillus plantarum is likely to be due to the fact that it contains rich protein, which protects the protein in the biofilm of the Lactobacillus plantarum; tea polyphenol plays an antioxidant role to reduce the damage to the cells; sodium tripolyphosphate acts as a water-retaining agent and thickening agent to stabilize the cells and the solution. The Lactobacillus plantarum slurry is prepared into the biological deodorant B liquid by using the specific protective agent and the nutrient solution system, and the problem of short shelf life of the Lactobacillus plantarum can be effectively solved.

[0149] The above examples are merely examples for clearly illustrating but not limiting the embodiments. Based on the above description, other different forms of changes or variations can be made by those skilled in the art. Here, all the embodiments cannot be exhausted, and the changes or variations derived therefrom are still within the protection scope of the present application.

Claims

1. A composite biological deodorant, characterized by, By weight parts, including the following components: Bacillus coagulans slurry 1-3 parts; Saccharomyces cerevisiae slurry 1-3 parts; Lactobacillus plantarum slurry 1-3 parts; Nutrient solution A liquid 10-30 parts; Nutrient solution B liquid 20-60 parts; The bacillus coagulans slurry is a solid material obtained by removing supernatant after fermentation and centrifugation of bacillus coagulans with the preservation number of CICC 23843, and the viable bacterial count is 5×10 9 9×10 9 cfu / g. The solid material obtained by fermenting and centrifuging Saccharomyces cerevisiae with the preservation number of CICC 1450, and removing supernatant, has a viable cell count of 1×10 9 5×10 9 cfu / g. The Lactobacillus plantarum bacterial slurry is a solid material obtained by removing supernatant after fermentation and centrifugation of Lactobacillus plantarum with the preservation number of CICC 21791, and the viable bacterial count is 1×10 10 5×10 10 cfu / g. The composition of the nutrient solution A liquid includes: sodium acetate 12-15 g / L, ammonium sulfate 1.5-2 g / L, sodium dihydrogen phosphate 0.9-1.1 g / L, disodium hydrogen phosphate 0.9-1.1 g / L, potassium chloride 3-5 g / L, magnesium sulfate 0.08-0.12 g / L, manganese sulfate 0.04-0.05 g / L, trace element supplement 0.22-0.32 g / L; the pH of the nutrient solution A liquid is 4.5-5; The composition of the nutrient solution B liquid includes: glucose 15-20 g / L, sodium acetate 3-5 g / L, protein peptone 8-12 g / L, Tween 800.8-1.2 ml / L, sodium dihydrogen phosphate 0.9-1.1 g / L, disodium hydrogen phosphate 0.9-1.1 g / L, potassium chloride 3-5 g / L, magnesium sulfate 0.08-0.12 g / L, manganese sulfate 0.04-0.05 g / L, the pH of the nutrient solution B liquid is 4.5-5; The preparation method of the Bacillus coagulans slurry includes the following steps: inoculating Bacillus coagulans into LB culture medium at an inoculation amount of 0.5-1.1%, and culturing at 28-35°C and 160-180 rpm / min for 24-30 h to obtain seed liquid A; inoculating seed liquid A into Bacillus coagulans fermentation culture medium at an inoculation amount of 2-5%, and culturing at 40-45°C and 160-180 rpm / min for 30-36 h to obtain Bacillus coagulans fermentation liquid; centrifuging the Bacillus coagulans fermentation liquid at a speed of 5000-6000 rpm / min, discarding the supernatant, and obtaining Bacillus coagulans slurry; the composition of the LB culture medium includes protein peptone 10.0 g / L, yeast powder 5.0 g / L, and NaCl 10.0 g / L; the composition of the Bacillus coagulans fermentation culture medium includes molasses 8.0-10.0 g / L, yeast extract powder 18.0-20.0 g / L, NaCl 4.0-5.0 g / L, K2HPO4 4.0-5.0 g / L, and MnSO4 8.0-10.0 mg / L; The preparation method of the Saccharomyces cerevisiae slurry comprises the following steps: inoculating Saccharomyces cerevisiae into YPD culture medium at an inoculation amount of 0.5-1%, and culturing at 28-35 DEG C and 160-180 rpm / min for 24-30 h to obtain seed liquid B; inoculating the seed liquid B into YPD culture medium at an inoculation amount of 1-3%, and culturing at 25-30 DEG C and 160-180 rpm / min, when the pH reduction rate is less than 0.01, adding sodium acetate solution to the fermentation system to make the concentration of sodium acetate in the fermentation system 7-10 g / L at one time, and continuing to ferment until the pH change rate is less than 0.01, to obtain a Saccharomyces cerevisiae fermentation liquor; centrifuging the Saccharomyces cerevisiae fermentation liquor at a speed of 3000-4000 rpm / min, and discarding the supernatant to obtain the Saccharomyces cerevisiae slurry; the YPD culture medium comprises the following components: glucose 20.0 g / L, yeast extract 10.0 g / L, and peptone 20.0 g / L. The preparation method of the Lactobacillus plantarum slurry comprises the following steps: inoculating Lactobacillus plantarum into MRS culture medium at an inoculation amount of 3-5%, and culturing at 28-35 DEG C for 40-48 h to obtain seed liquid C; inoculating the seed liquid C into MRS culture medium at an inoculation amount of 5-10%, and culturing at 28-35 DEG C for 40-48 h to obtain Lactobacillus plantarum fermentation liquor, centrifuging the Lactobacillus plantarum fermentation liquor at a speed of 3000-3500 rpm / min, and discarding the supernatant to obtain the Lactobacillus plantarum slurry; the MRS culture medium comprises the following components: peptone 10.0 g / L, beef extract 5.0 g / L, yeast extract 4.0 g / L, triammonium citrate 2.0 g / L, glucose 20.0 g / L, Tween 80 1 ml, sodium acetate 5.0 g / L, potassium phosphate dibasic 2.0 g / L, magnesium sulfate 0.2 g / L, and manganese sulfate 0.05 g / L, and the pH is 6.4±0.

2.

2. The composite biological deodorizer according to claim 1, characterized by, The trace element supplement in the nutrient solution A liquid comprises at least one of disodium ethylenediaminetetraacetate, zinc sulfate heptahydrate, boric acid, manganese chloride tetrahydrate, cobalt chloride hexahydrate, copper sulfate pentahydrate, ammonium molybdate tetrahydrate, and ferrous sulfate heptahydrate.

3. The composite biological deodorizer according to claim 2, wherein The content of the trace element supplement in the nutrient solution A liquid is as follows: disodium ethylenediaminetetraacetate 0.05-0.075 g / L, zinc sulfate heptahydrate 0.022-0.033 g / L, boric acid 0.0114-0.171 g / L, manganese chloride tetrahydrate 0.0506-0.0759 g / L, cobalt chloride hexahydrate 0.0161-0.02415 g / L, copper sulfate pentahydrate 0.0157-0.02355 g / L, ammonium molybdate tetrahydrate 0.011-0.0165 g / L, and ferrous sulfate heptahydrate 0.0499 g / L.

4. The composite biological deodorizer according to claim 1, wherein The nutrient solution B solution further comprises cyclodextrin, CITRUS RETICULATA peel extract and citric acid; the cyclodextrin content in the nutrient solution B solution is 0.8-1.2 g / L, the CITRUS RETICULATA peel extract content is 3-5 g / L, and the pH of the nutrient solution is adjusted to 4.5-5 by citric acid.

5. The composite biological deodorizer of claim 1, wherein The biological deodorant comprises a biological deodorant A solution and a biological deodorant B solution. The Bacillus coagulans slurry, the Saccharomyces cerevisiae slurry and the nutrient solution A solution constitute the biological deodorant A solution. The Lactobacillus plantarum slurry and the nutrient solution B solution constitute the biological deodorant B solution.

6. The composite biological deodorizer according to claim 5, wherein The biological deodorant B solution further comprises 1.5-3 wt% mannitol, 0.6-1 wt% inulin, 0.8-1.5 wt% skimmed milk, 0.03-0.06 wt% tea polyphenols and 0.02-0.05 wt% sodium tripolyphosphate.

7. A process for the preparation of the composite bio-deodorant of any one of claims 1 to 4, characterized in that, The method comprises the following steps: mixing the components according to the proportions to obtain the composite biological deodorant.

8. The method of claim 5, wherein the composite bio-deodorizer is prepared by the steps of: The method comprises the following steps: S1. Preparing the biological deodorant A solution: The biological deodorant A solution is prepared by mixing the Bacillus coagulans slurry, the Saccharomyces cerevisiae slurry and the nutrient solution A solution according to the proportions; S2. Preparing the biological deodorant B solution: The biological deodorant B solution is prepared by mixing the Lactobacillus plantarum slurry and the nutrient solution B solution according to the proportions; The biological deodorant A solution and the biological deodorant B solution are stored in different containers respectively, and the composite biological deodorant is obtained by mixing the biological deodorant A solution and the biological deodorant B solution when used.

9. A deodorizing spray, characterized by, The composite biological deodorant according to any one of claims 1-6.

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