Microorganism detection and analysis device

Abstract

The invention discloses a microbiological detection and analysis device which comprises a supporting table, an outer cover is fixedly arranged on the upper side of the supporting table, at least one window is formed in the outer cover, a culture dish shaking assembly is arranged in the outer cover, and the culture dish shaking assembly is used for supporting a culture dish and shaking the culture dish. A movable liquid adding assembly is further arranged in the outer cover, and the movable liquid adding assembly is arranged on the upper side of the culture dish shaking assembly and can perform liquid adding operation on a culture dish placed in the culture dish placing assembly. The invention relates to the technical field of microbiological detection equipment, in particular to a microbiological detection and analysis device. Shaking and liquid adding operation is achieved through the culture dish shaking assembly and the movable liquid adding assembly, the culture dish shaking assembly converts circular motion of a motor into shaking motion of a culture dish containing groove through an eccentric wheel mechanism, manual shaking is simulated, the force and frequency are stable, and it is ensured that a culture medium and microorganisms are fully mixed.

Description

Technical Field

[0001] This invention relates to the field of microbial detection equipment technology, and more specifically, to a microbial detection and analysis device. Background Art

[0002] Microbial detection and analysis is the process of using specialized techniques and equipment to isolate, culture, identify, count, and analyze the characteristics of microorganisms (such as bacteria, fungi, and viruses) in food, environmental, and medical samples.

[0003] Its core objectives include determining the number and types of microorganisms, understanding their physiological and biochemical characteristics, pathogenicity, and drug resistance, thereby providing scientific basis for fields such as food safety monitoring, biopharmaceuticals, disease diagnosis, and environmental quality assessment.

[0004] Traditional detection processes often rely on manual operation. Shaking the petri dishes is usually done manually, which is time-consuming, labor-intensive, and requires continuous operation. Adding liquid also relies on manual operation, which can easily cause the liquid to shake onto the lid, increasing the chance of contamination. This is inefficient and wastes manpower. Therefore, a microbial detection and analysis device was designed that can mimic manual shaking with stable force and frequency to ensure that the culture medium and microorganisms are fully mixed. Summary of the Invention

[0005] This invention provides a microbial detection and analysis device. The invention realizes the shaking and liquid addition operations through a petri dish shaking component and a moving liquid addition component. The petri dish shaking component uses an eccentric wheel mechanism to convert the circular motion of the motor into the shaking motion of the petri dish placement tank, which imitates manual shaking and has stable force and frequency, ensuring that the culture medium and microorganisms are fully mixed.

[0006] A microbial detection and analysis device includes a support platform, an outer cover fixedly mounted on the upper side of the support platform, at least one window on the outer cover, a culture dish shaking assembly disposed inside the outer cover for supporting and shaking the culture dish, and a movable liquid addition assembly disposed inside the outer cover, the movable liquid addition assembly being disposed above the culture dish shaking assembly and capable of adding liquid to the culture dish placed inside the culture dish placement assembly.

[0007] Furthermore, a fixing plate is fixedly installed inside the outer cover. The fixing plate is L-shaped. The vertical plate of the fixing plate fixes the support platform, the horizontal plate of the fixing plate is fixedly connected to the culture dish shaking assembly, and the upper side of the horizontal plate of the fixing plate is fixedly connected to the movable liquid addition assembly.

[0008] Furthermore, the culture dish shaking assembly includes two symmetrically arranged sets of culture dish placement slots, each set containing several culture dish placement slots, and a sphere is fixedly connected to the lower side of each culture dish placement slot. The sphere is rotatably disposed within the sphere slot, which is located on a support plate. The support plate is fixedly connected to the horizontal end of a fixed plate. A fixed rod is fixedly connected to the lower side of each sphere. The fixed rod is L-shaped, and its vertical section fixes the sphere. The horizontal end of each set of fixed rods is rotatably connected to a first connecting rod. The lower center of the first connecting rod is fixedly connected to one end of the vertical rod of a crank arm. One end of the horizontal rod of each of the two crank arms is rotatably connected to one end of a moving rod. The top of the moving rod, protruding to one side, is rotatably connected to the eccentric shaft of a rotating wheel. The center of the rotating wheel is fixedly connected to the output shaft of a second motor, which is fixed to the lower side of the support plate.

[0009] Furthermore, the mobile liquid dispensing assembly includes a linear motor, the movable end of which is fixedly connected to two liquid outlet pump heads. The inlets of the liquid outlet pump heads are respectively fixedly connected to one end of a liquid inlet pipe. Each liquid inlet pipe is respectively fixedly connected to the outlet of a liquid storage cylinder through a flexible tube. The liquid storage cylinder is fixed to the outer wall of the outer cover. The fixed end of the linear motor is fixedly connected to the movable end of a linear guide rail through a connecting block. The fixed end of the linear guide rail is fixedly connected to the horizontal plate of the fixed plate through a vertical plate.

[0010] Furthermore, the movable end of the linear guide is fixedly connected to a convex shaft, the convex shaft is slidably fitted in the groove of the rotating groove rod, one end of the rotating groove rod is fixedly connected to the output shaft of the first motor, the first motor is fixedly connected to the fixed plate and the upright plate, and the output shaft of the first motor is rotatably connected to the upright plate.

[0011] Furthermore, a collection trough is provided inside the outer cover, the collection trough is fixed on the support plate, a liquid outlet pipe is fixedly provided at the lower end of one side of the collection trough, a valve is provided at the outer end of the liquid outlet pipe, and the liquid outlet pipe is fixedly passed through the outer cover.

[0012] Furthermore, the outer cover is made of a transparent material.

[0013] Compared with the prior art, the advantages and positive effects of the present invention are: The device realizes the shaking and liquid addition operations through the petri dish shaking component and the moving liquid addition component. The petri dish shaking component uses an eccentric wheel mechanism to convert the circular motion of the motor into the shaking motion of the petri dish placement tank, which imitates manual shaking and has stable force and frequency, ensuring that the culture medium and microorganisms are fully mixed. The mobile liquid dispensing assembly uses a linear motor, linear guide rail and other structures to achieve precise movement and liquid dispensing of the dispensing pump head, replacing manual operation and making it more convenient; The transparent outer cover allows operators to observe the internal situation in real time without having to open it frequently, reducing the risk of contamination; the collection tank and liquid outlet pipe can collect and discharge liquid in a timely manner, preventing liquid leakage from contaminating the equipment or the environment. Attached Figure Description

[0014] The accompanying drawings, which are included to provide a further understanding of this application and form part of this application, illustrate exemplary embodiments and are used to explain this application, but do not constitute an undue limitation of this application. Obviously, the drawings described below are merely some embodiments of the present invention, and those skilled in the art can obtain other drawings based on these drawings without creative effort. In the drawings: Figure 1 The three-dimensional representation of the present invention Figure 1 ; Figure 2 The three-dimensional representation of the present invention Figure 2 ; Figure 3 This is a partial three-dimensional representation of the present invention. Figure 1 ; Figure 4 This is a partial three-dimensional representation of the present invention. Figure 2 ; Figure 5 This is a partial three-dimensional representation of the present invention. Figure 3 ; Figure 6 This is a three-dimensional perspective view of the present invention.

[0015] In the diagram: 1. Outer cover; 101. Window; 2. Support platform; 201. Storage tank; 3. Electric lifting table leg; 4. Support base; 5. Liquid storage cylinder; 501. End cap; 6. Liquid outlet pipe; 601. Valve; 7. Moving liquid addition assembly; 702. First motor; 703. Rotating groove rod; 704. Vertical plate; 705. Linear guide rail; 706. Convex shaft; 707. Connecting block; 708. Linear motor; 709. Liquid outlet pump head; 7091. Liquid inlet pipe; 8. Fixing plate; 9. Petri dish shaking assembly; 901. Petri dish placement tank; 902. Sphere; 903. Support plate; 904. Ball groove; 905. First connecting rod; 906. Fixing rod; 907. Crank arm; 908. Moving rod; 909. Rotating wheel; 10. Second motor; 11. Collection tank. Detailed Implementation

[0016] The following is in conjunction with the appendix Figures 1-5 The present invention will be further described in conjunction with the embodiments.

[0017] The specific embodiments of the present invention are as follows: A microbial detection and analysis device includes a support platform 2, an outer cover 1 fixedly disposed on the upper side of the support platform 2, at least one window 101 opened on the outer cover 1, a culture dish shaking component 9 disposed inside the outer cover 1, the culture dish shaking component 9 being used to support the culture dish and shake the culture dish, and a movable liquid addition component 7 disposed inside the outer cover 1, the movable liquid addition component 7 being disposed above the culture dish shaking component 9 and being able to add liquid to the culture dish placed inside the culture dish placement component.

[0018] In this embodiment, the culture dish is supported by the culture dish shaking assembly 9 fixed inside the outer cover 1 and the culture dish is shaken; at the same time, the liquid addition assembly 7 located on the upper side of the culture dish shaking assembly 9 is used to add liquid to the culture dish. The window 101 on the outer cover 1 can be used to observe the internal situation or to place or remove the culture dish.

[0019] A fixing plate 8 is fixedly installed inside the outer cover 1. The fixing plate 8 is L-shaped. The vertical plate of the fixing plate 8 fixes the support platform 2, and the horizontal plate of the fixing plate 8 is fixedly connected to the culture dish shaking assembly 9. The upper part of the horizontal plate of the fixing plate 8 is fixedly connected to the movable liquid addition assembly 7. The L-shaped fixing plate 8, with its vertical plate fixed to the support platform 2, serves to connect the support platform 2 to the internal components of the outer cover 1, while the horizontal plate is fixed to both the culture dish shaking assembly 9 and the movable liquid addition assembly 7.

[0020] Furthermore, the culture dish shaking assembly 9 includes two symmetrically arranged sets of culture dish placement slots 901, each set containing several slots. A sphere 902 is fixedly connected to the lower side of each culture dish placement slot 901. The sphere 902 is rotatably disposed within a ball groove 904, which is formed on a support plate 903. The support plate 903 is fixedly connected to the end of the horizontal plate of the fixing plate 8. A fixing rod 906, L-shaped, is fixedly connected to the lower side of each sphere 902. The sphere 902 is fixed by a vertical rod. The horizontal end of each set of fixed rods 906 is rotatably connected to a first connecting rod 905. The lower center of the first connecting rod 905 is fixedly connected to one end of the vertical rod of the crank arm 907. The horizontal ends of the two crank arms 907 are rotatably connected to both ends of one side of the moving rod 908. The top of the moving rod 908, which protrudes to one side, is rotatably connected to the eccentric shaft of the rotating wheel 909. The center of the rotating wheel 909 is fixedly connected to the output shaft of the second motor 10. The second motor 10 is fixed to the lower side of the support plate 903.

[0021] In this embodiment, the second motor 10 drives the rotating wheel 909 to rotate. The eccentric shaft of the rotating wheel 909 drives the moving rod 908 to reciprocate. The moving rod 908 drives the fixed rod 906 to move through the crank arm 907 and the first connecting rod 905. The fixed rod 906 then rotates within the ball groove 904 through the ball 902, thereby causing the culture dish placement tank 901 to shake. The eccentric wheel mechanism converts the circular motion of the motor into the shaking motion of the culture dish placement tank 901, realizing the shaking function of the culture dish and mimicking the operation of manually shaking the culture dish. By shaking the culture dish, the culture medium and microorganisms in the culture dish can be fully mixed, which is conducive to the uniform contact of microorganisms with nutrients, promotes their growth, and also helps some chemical reactions or detection processes to proceed more fully.

[0022] Furthermore, the mobile liquid addition assembly 7 includes a linear motor 708, the movable end of which is fixedly connected to two liquid outlet pump heads 709. The inlets of the liquid outlet pump heads 709 are respectively fixedly connected to one end of the liquid inlet pipes 7091. Each liquid inlet pipe 7091 is respectively fixedly connected to the outlet of the liquid storage cylinder 5 through a flexible tube. The liquid storage cylinder 5 is fixed to the outer wall of the outer cover 1. The fixed end of the linear motor 708 is fixedly connected to the movable end of the linear guide rail 705 through a connecting block 707. The fixed end of the linear guide rail 705 is fixedly connected to the horizontal plate of the fixed plate 8 through a vertical plate 704.

[0023] In this embodiment, the linear motor 708 drives the movable end to move, thereby moving the liquid dispensing pump head 709 on the linear guide rail 705. The liquid dispensing pump head 709 draws liquid from the storage cylinder 5 through the liquid inlet pipe 7091 and the hose, and adds the liquid to the culture dish as needed. The linear motor 708 and the linear guide rail 705 realize the linear movement of the liquid dispensing pump head 709, and the liquid addition operation is realized by the liquid suction and liquid dispensing functions of the pump head, which can accurately add liquid to the culture dish.

[0024] Furthermore, the movable end of the linear guide 705 is fixedly connected to the convex shaft 706, the convex shaft 706 is slidably fitted in the groove of the rotating groove rod 703, one end of the rotating groove rod 703 is fixedly connected to the output shaft of the first motor 702, the first motor 702 is fixedly connected to the fixed plate 8 and the upright plate 704, and the output shaft of the first motor 702 is rotatably connected to the upright plate 704.

[0025] In this embodiment, the first motor 702 drives the rotating groove rod 703 to rotate. The groove of the rotating groove rod 703 cooperates with the convex shaft 706 at the movable end of the linear guide rail 705, causing the movable end of the linear guide rail 705 to reciprocate along the movable end of the linear guide rail 705 under the action of the rotating groove rod 703. This allows the liquid dispensing pump head 709 to more flexibly reach the top of the culture dish at different positions for liquid addition. The first motor 702 is a stepper motor.

[0026] Furthermore, a collection trough 11 is provided inside the outer cover 1. The collection trough 11 is fixed on the support plate 903. A liquid outlet pipe 6 is fixedly provided at the lower end of one side of the collection trough 11. A valve 601 is provided at the outer end of the liquid outlet pipe 6. The liquid outlet pipe 6 is fixedly passed through the outer cover 1.

[0027] In this embodiment, the collection tank 11 is used to collect the liquid discharged when emptying the liquid storage cylinder 5 and when cleaning the liquid storage cylinder 5. The liquid is discharged from the outer cover 1 through the liquid outlet pipe 6. The valve 601 is used to control the opening and closing of the liquid outlet pipe 6. The valve 601 can be opened to discharge the liquid when needed, and closed to prevent liquid leakage when not needed.

[0028] Furthermore, the outer cover 1 is made of a transparent material.

[0029] The transparent material can be glass or transparent acrylic. The outer cover 1 is made of transparent material, allowing light to pass through, so that operators can directly observe the state of the culture dish inside the outer cover 1, the liquid addition, and the growth of microorganisms from the outside without opening the outer cover 1.

[0030] A color-changing spectral light-emitting module (not shown in the figure, existing technology, and will not be shown again) is fixedly installed on the upper inner side of the outer cover 1. This module uses Luminus' RGBWW variable spectrum module, which consists of multiple sets of switchable wavelength LED beads. The spectrum of the beads covers the visible light and near-ultraviolet bands commonly used in microbial detection. Its luminous intensity can be precisely adjusted by the control system. This module is fixed to the upper inner wall of the outer cover 1, located directly above the petri dish shaking component 9. It can emit light of a specific wavelength into the sample in the petri dish during the microbial culture or reaction stage. By utilizing the differences in the absorption or reflection characteristics of specific spectra by microorganisms under different growth states, and in conjunction with an external spectral detection sensor (which can be integrated into the corresponding position on the inner wall of the outer cover 1), it can quickly and preliminarily determine the type, concentration, or metabolic state of microorganisms, providing preprocessing data support for subsequent accurate detection.

[0031] The outer casing 1 also houses a heating module, which utilizes a Hanuo350 constant-temperature heating element (not shown in the figure, existing technology, and therefore not presented), manufactured by Shanghai Hanuo Instruments Co., Ltd. The Hanuo350 constant-temperature heating element boasts excellent temperature control performance, ensuring accurate and stable temperature output. The heating element is arranged in a ring on the inner wall of the outer casing 1, maintaining a preset safe distance from the culture dish shaking component 9 to prevent localized high-temperature burns to the culture dish. The heating module monitors the internal ambient temperature of the outer casing 1 in real time via a temperature sensor and feeds the data back to the temperature control unit. It can achieve precise temperature control within the range of 15-60℃ (±0.5℃), providing a stable constant-temperature environment for microbial culture, meeting the specific temperature requirements of different types of microorganisms, and ensuring the stability and repeatability of the culture process.

[0032] The outer casing 1 is also equipped with an ultraviolet disinfection module. The ultraviolet disinfection module uses a high-intensity ultraviolet lamp with a wavelength of 254nm (not shown in the figure, existing technology, and will not be shown again). It is fixed to the corner of the top inside the outer casing 1 by a lamp holder. Before or after the device performs the detection operation, the ultraviolet lamp can irradiate and disinfect the internal space of the outer casing 1, the petri dish placement component, and the exposed parts of the movable liquid addition component 7. The strong bactericidal effect of ultraviolet light destroys the DNA structure of microorganisms, effectively kills residual bacteria, avoids cross-contamination when testing different batches of samples, and ensures the sterility of the detection environment.

[0033] The support platform 2 is also fixedly provided with a storage slot 201 for storing culture dishes, etc.

[0034] The support platform 2 is equipped with electrically adjustable table legs 3 at its four lower corners. The electrically adjustable table legs 3 adopt the DISRAD EasLift™ electric bracket to facilitate the raising and lowering of the support platform 2.

[0035] The method of using this invention is as follows: First, check whether each component of the device is normal, and ensure that the outer cover 1 is intact and that the ultraviolet disinfection module, heating module, color-changing spectrum luminescence module, etc. are all working properly; The operator puts on protective clothing and a protective mask, and places the petri dish containing microbial samples and culture medium into the petri dish shaking component 9 and places the petri dish into the tank through the window 101 on the outer cover 1; Activate the ultraviolet disinfection module to disinfect the internal space of the device, the petri dish placement components and the exposed parts of the movable liquid addition component 7 by irradiation. After disinfection is completed, turn off the ultraviolet disinfection module. Based on the cultivation requirements of microorganisms, the target temperature of the heating module is set through the control system so that the internal environment temperature of the outer casing 1 reaches the suitable temperature required for microbial growth; at the same time, the emission wavelength and intensity of the color-changing spectral emission module are set according to the detection requirements. The liquid to be added is poured into the storage cylinder 5. The control system starts the moving liquid addition component 7. The first motor 702 drives the rotating groove rod 703 to rotate, which drives the movable end of the linear guide rail 705 to move, thereby moving the linear motor 708 and the liquid dispensing pump head 709 above the target culture dish. The linear motor 708 drives the liquid dispensing pump head 709 to adjust its position. The liquid dispensing pump head 709 draws liquid from the storage cylinder 5 through the liquid inlet pipe 7091 and the hose, and adds the liquid precisely into the culture dish. After the liquid addition is completed, the moving liquid addition component 7 is reset. The second motor 10 of the petri dish shaking assembly 9 is activated. The second motor 10 drives the rotating wheel 909 to rotate. The eccentric shaft of the rotating wheel 909 drives the moving rod 908 to reciprocate. The moving rod 908 drives the fixed rod 906 to move through the crank arm 907 and the first connecting rod 905. The fixed rod 906 rotates in the ball groove 904 through the ball 902, thereby shaking the petri dish placement groove 901 to fully mix the culture medium and microorganisms in the petri dish. The shaking time and frequency are set according to the culture requirements. After shaking is completed, the second motor 10 is turned off. During the cultivation process, the growth of microorganisms in the culture dish can be observed through the transparent outer cover 1; the color-changing spectral luminescence module emits light of a specific wavelength to the sample, and in conjunction with the spectral detection sensor, the type, concentration or metabolic state of microorganisms can be preliminarily determined, and the relevant data can be transmitted to the control system in real time for the operator to view; After the test is completed, open the outer cover 1 window 101 and take out the culture dish. If there is residual liquid in the storage cylinder 5 or the storage cylinder 5 needs to be cleaned, control the operation of the moving liquid addition component 7 to drive the liquid outlet pump head 709 to move above the collection tank 11, discharge the liquid into the collection tank 11, open the valve 601 of the corresponding liquid outlet pipe 6 of the collection tank 11, discharge the liquid into the collection tank 11 and discharge it through the liquid outlet pipe 6; Finally, the ultraviolet disinfection module is activated again to disinfect the inside of the device for future use.

[0036] The above description is merely an embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structural or procedural transformations made based on the content of the present invention specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of the present invention.

Claims

1. A microbial detection and analysis device, comprising a support platform (2), characterized in that, An outer cover (1) is fixedly installed on the upper side of the support platform (2). At least one window (101) is opened on the outer cover (1). A culture dish shaking component (9) is installed inside the outer cover (1). The culture dish shaking component (9) is used to support the culture dish and shake the culture dish. A movable liquid addition component (7) is also installed inside the outer cover (1). The movable liquid addition component (7) is installed on the upper side of the culture dish shaking component (9) and can add liquid to the culture dish placed in the culture dish placement component.

2. The microbial detection and analysis device according to claim 1, characterized in that: A fixing plate (8) is fixedly installed inside the outer cover (1). The fixing plate (8) is L-shaped. The vertical plate of the fixing plate (8) fixes the support platform (2). The horizontal plate of the fixing plate (8) is fixedly connected to the culture dish shaking assembly (9). The upper side of the horizontal plate of the fixing plate (8) is fixedly connected to the movable liquid addition assembly (7).

3. The microbial detection and analysis device according to claim 2, characterized in that: The petri dish shaking assembly (9) includes two symmetrically arranged petri dish placement slots (901), each set of petri dish placement slots (901) having several slots, and each petri dish placement slot (901) has a sphere (902) fixedly connected to its lower side. The sphere (902) is rotatably disposed within a ball groove (904), which is located on a support plate (903). The support plate (903) is fixedly connected to the end of the horizontal plate of the fixing plate (8). The lower side of each sphere (902) is fixedly connected to a fixing rod (906), which is L-shaped. The sphere (902) is fixed by a vertical rod. The horizontal end of each set of fixed rods (906) is rotatably connected to a first connecting rod (905). The lower center of the first connecting rod (905) is fixedly connected to one end of the vertical rod of the crank arm (907). The horizontal ends of the two crank arms (907) are rotatably connected to the two ends of one side of the moving rod (908). The top of the moving rod (908) protruding to one side is rotatably connected to the eccentric shaft of the rotating wheel (909). The center of the rotating wheel (909) is fixedly connected to the output shaft of the second motor (10). The second motor (10) is fixed to the lower side of the support plate (903).

4. The microbial detection and analysis device according to claim 3, characterized in that: The mobile liquid addition assembly (7) includes a linear motor (708). The movable end of the linear motor (708) is fixedly connected to two liquid outlet pump heads (709). The inlet of each liquid outlet pump head (709) is fixedly connected to one end of an inlet pipe (7091). Each inlet pipe (7091) is fixedly connected to the outlet of a storage cylinder (5) via a hose. The storage cylinder (5) is fixed to the outer wall of the outer cover (1). The fixed end of the linear motor (708) is fixedly connected to the movable end of a linear guide rail (705) via a connecting block (707). The fixed end of the linear guide rail (705) is fixedly connected to the horizontal plate of the fixed plate (8) via a vertical plate (704).

5. The microbial detection and analysis device according to claim 4, characterized in that: The movable end of the linear guide (705) is fixedly connected to the convex shaft (706), and the convex shaft (706) is slidably fitted in the groove of the rotating groove rod (703). One end of the rotating groove rod (703) is fixedly connected to the output shaft of the first motor (702). The first motor (702) is fixedly connected to the fixed plate (8) and the upright plate (704), and the output shaft of the first motor (702) is rotatably connected to the upright plate (704).

6. A microbial detection and analysis device according to claim 5, characterized in that: The outer cover (1) is provided with a collection tank (11), which is fixed on the support plate (903). A liquid outlet pipe (6) is fixedly provided at the lower end of one side of the collection tank (11), and a valve (601) is provided at the outer end of the liquid outlet pipe (6). The liquid outlet pipe (6) is fixedly passed through the outer cover (1).

7. A microbial detection and analysis device according to claim 6, characterized in that: The outer cover (1) is made of transparent material.

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