Multifunctional microplate reader

Abstract

The invention relates to the technical field of microplate readers, and discloses a multifunctional microplate reader which comprises a full-wavelength microplate reader body, a detection cavity used for detecting a sample is formed in the full-wavelength microplate reader body, and a microplate used for placing the sample is arranged in the detection cavity. The full-wavelength microplate reader body is rotationally connected with a cover plate for sealing the detection cavity through a rotating shaft, the full-wavelength microplate reader body is further provided with a control panel for controlling the device to be opened and closed, a sample taking and placing assembly is arranged in the detection cavity, and a cleaning assembly is arranged on the sample taking and placing assembly. And a wiping assembly is arranged on the microporous plate. The device effectively avoids the problem that the accuracy of a sample detection result is affected due to the fact that an existing device is difficult to clean the surface of the microporous plate, and is high in practicability.

Description

Technical Field

[0001] This invention relates to the field of enzyme-linked immunosorbent assay (ELISA) readers, and more particularly to a multifunctional ELISA reader. Background Technology

[0002] Microplate readers (ELISA readers) are mainly used to detect specific substances in biological samples, such as proteins, antibodies, and antigens. They measure the concentration or activity of target substances in samples using optical principles and are widely used in scientific research, medical diagnostics, and other fields.

[0003] When an ELISA reader is testing samples, external dust inevitably gets introduced into the microplate inside the detection chamber. In addition, the device lacks an integrated structure for cleaning the microplate, which makes the dust adhering to the microplate easily affect the accuracy of subsequent sample detection results. To address this, we provide a multifunctional ELISA reader. Summary of the Invention

[0004] To address the problem in the aforementioned background technology that the difficulty in cleaning the surface of microplates affects the accuracy of sample detection results, this invention provides a multifunctional enzyme-linked immunosorbent assay (ELISA) reader.

[0005] The present invention is achieved by the following technical solution: a multifunctional microplate reader, comprising a full-wavelength microplate reader body, wherein the full-wavelength microplate reader body has a detection chamber for detecting samples, and a microplate for placing samples is disposed inside the detection chamber. A cover plate for sealing the detection chamber is rotatably connected to the full-wavelength microplate reader body via a rotating shaft. The full-wavelength microplate reader body is also provided with a control panel for controlling the opening and closing of the control device. A sample pick-and-place component is disposed inside the detection chamber, and a cleaning component is disposed on the sample pick-and-place component. A wiping component is disposed on the microplate.

[0006] As a further improvement to the above solution, the sample handling assembly includes a motor fixedly connected to the full-wavelength microplate reader body. The rotor end of the motor is coaxially fixedly connected to a connecting shaft, which is rotatably connected to the full-wavelength microplate reader body. A bidirectional lead screw is rotatably connected inside the detection chamber, and one end of the bidirectional lead screw is coaxially fixedly connected to the connecting shaft. Movable plates are threaded to both sides of the bidirectional lead screw. A horizontal plate is movably arranged below the microplate. Two sets of connecting rods are symmetrically hinged between the horizontal plate and the movable plate. A guide rod is fixedly connected inside the detection chamber, and the guide rod is slidably connected to both sets of movable plates.

[0007] As a further improvement to the above solution, the cleaning component includes a scraper disposed below the moving plate and in contact with the bottom of the inner cavity of the detection chamber. Two sets of fixing rods are symmetrically fixedly connected to the top of the scraper and are slidably connected to the moving plate on the same side. A limiting plate is fixedly connected to the top of the fixing rod, and a spring is sleeved on the outside of the fixing rod. Three sets of movable cavities are equidistantly opened on the full-wavelength microplate reader body. A strip-shaped groove is fixedly connected between the movable cavity and the detection chamber. A collection box is slidably connected inside the movable cavity.

[0008] As a further improvement to the above solution, the wiping assembly includes four sets of vertical rods symmetrically fixedly connected to the bottom of the microporous plate and slidably connected to the horizontal plate. The bottom end of each vertical rod is fixedly connected to a limiting plate II. A spring II is sleeved on the outside of each vertical rod. The top of the horizontal plate is symmetrically fixedly connected to four sets of connecting seats and slidably connected to the microporous plate. A one-way screw is rotatably connected between two sets of connecting seats on one side. A guide rod II is fixedly connected between two sets of connecting seats on the other side. A moving plate II is threadedly connected to the outside of the one-way screw and slidably connected to the guide rod II. A wiping cotton is provided at the bottom of the moving plate II. Gears are coaxially fixedly connected to both ends of the one-way screw. Two sets of racks are symmetrically fixedly connected to the inner wall of the detection cavity and mesh with the gears on the same side.

[0009] As a further improvement to the above solution, rectangular through holes are uniformly formed on the horizontal plate, and the rectangular through holes correspond to the holes on the microporous plate.

[0010] As a further improvement to the above solution, the length of the scraper is matched with the width of the detection chamber.

[0011] As a further improvement to the above solution, the length of the second movable plate is matched with the width of the microporous plate.

[0012] As a further improvement to the above solution, the wiping cotton is fixedly connected to the second movable plate by adhesive bonding.

[0013] As a further improvement to the above solution, the two ends of the spring are fixedly connected to the limiting plate and the moving plate, respectively.

[0014] As a further improvement to the above solution, the two ends of the second spring are fixedly connected to the micro-perforated plate and the horizontal plate, respectively.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0016] 1. This invention uses a motor to drive a bidirectional lead screw to rotate, and through the sliding engagement between the first moving plate and the first guide rod, it enables two sets of first moving plates to move in opposite directions. Utilizing the hinged engagement between the first moving plates, the horizontal plate, and the connecting rod, the two sets of first moving plates can push the horizontal plate upward when moving in opposite directions, thereby pushing the microplate upward to facilitate sample placement. Conversely, it can stably drive the sample into the detection chamber, making it highly practical.

[0017] 2. When the two sets of moving plates move towards or away from each other, the scraper below them can also move together, thereby cleaning the bottom of the detection chamber. The scraped sample will enter the collection box through the strip groove, thus avoiding sample spillage and residue inside the full-wavelength microplate reader, which will affect its use.

[0018] 3. The present invention utilizes the sliding fit between the fixed rod and the movable plate, and the rebound effect of the spring, to ensure that the scraper is always in contact with the bottom of the inner cavity of the detection chamber, thereby ensuring the thorough cleaning of the bottom of the inner cavity of the detection chamber.

[0019] 4. This invention can drive two sets of gears to move together while the microporous plate moves vertically. The meshing between the gears and the rack drives the unidirectional lead screw to rotate. With the sliding cooperation between the second moving plate and the second guide rod, the second moving plate can move horizontally, thereby driving the wiping cotton to wipe and clean the surface of the microporous plate. It is highly practical.

[0020] 5. In this invention, after the glass container containing the sample is placed on the microplate, the increased weight and the sliding cooperation between the vertical rod and the horizontal plate cause the microplate to descend and compress the second spring. When the glass container is removed after the test, the reduced weight and the rebound action of the second spring cause the microplate to automatically reset. The vibration generated during the reset process can shake off the residue in the microplate, thereby improving the cleaning effect of the microplate.

[0021] 6. The present invention uses an adhesive method to connect and fix the wiping cotton to the second movable plate, which makes it easy to disassemble and replace the wiping cotton at any time, and is highly practical. Attached Figure Description

[0022] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0023] Figure 2 This is a longitudinal section three-dimensional structural diagram of the present invention;

[0024] Figure 3 This is a three-dimensional structural diagram of the wiping component of the present invention;

[0025] Figure 4This is a side view of the three-dimensional structure of the wiping assembly of the present invention;

[0026] Figure 5 This is a three-dimensional structural diagram of the sample handling component of the present invention;

[0027] Figure 6 This is a three-dimensional structural diagram of the cleaning component of the present invention.

[0028] Explanation of key symbols:

[0029] 1. Full-wavelength microplate reader body; 2. Detection chamber; 3. Microplate; 4. Cover plate; 5. Control panel; 101. Motor; 102. Connecting shaft; 103. Bidirectional lead screw; 104. Moving plate one; 105. Horizontal plate; 106. Connecting rod; 107. Guide rod one; 108. Rectangular through hole; 201. Scraper; 202. Fixing rod; 203. Limiting plate one; 204. Spring one; 205. Strip groove; 206. Collection box; 301. Vertical rod; 302. Limiting plate two; 303. Spring two; 304. Connecting seat; 305. Unidirectional lead screw; 306. Guide rod two; 307. Moving plate two; 308. Wiping cotton; 309. Gear; 310. Rack. Detailed Implementation

[0030] The present invention will now be further described in conjunction with the accompanying drawings and specific embodiments. It should be noted that, without conflict, the various embodiments or technical features described below can be arbitrarily combined to form new embodiments.

[0031] Example 1:

[0032] Please combine Figure 1-6 This embodiment of a multifunctional ELISA reader includes a full-wavelength ELISA reader body 1, a detection chamber 2 for detecting samples on the full-wavelength ELISA reader body 1, a microplate 3 for placing samples inside the detection chamber 2, a cover plate 4 for sealing the detection chamber 2 rotatably connected to the full-wavelength ELISA reader body 1 via a rotating shaft, a control panel 5 for controlling the opening and closing of the device on the full-wavelength ELISA reader body 1, a sample pick-and-place assembly inside the detection chamber 2, a cleaning assembly on the sample pick-and-place assembly, and a wiping assembly on the microplate 3.

[0033] The sample handling assembly includes a motor 101 fixedly connected to the full-wavelength microplate reader body 1. The rotor end of the motor 101 is coaxially fixedly connected to a connecting shaft 102, and the connecting shaft 102 is rotatably connected to the full-wavelength microplate reader body 1. A bidirectional lead screw 103 is rotatably connected inside the detection chamber 2, and one end of the bidirectional lead screw 103 is coaxially fixedly connected to the connecting shaft 102. Movable plates 104 are threaded to both sides of the bidirectional lead screw 103. A horizontal plate 105 is movably arranged below the microplate 3. Two sets of connecting rods 106 are symmetrically hinged between the horizontal plate 105 and the movable plate 104 through hinges. A guide rod 107 is fixedly connected inside the detection chamber 2, and the guide rod 107 is slidably connected to the two sets of movable plates 104. Rectangular through holes 108 are evenly opened on the horizontal plate 105, and the rectangular through holes 108 correspond to the holes on the microplate 3, so that the spilled sample solution can flow to the bottom of the inner cavity of the detection chamber 2 through the rectangular through holes 108.

[0034] The cleaning assembly includes a scraper 201 positioned below the moving plate 104, which contacts the bottom of the inner cavity of the detection chamber 2. Two sets of fixing rods 202 are symmetrically fixedly connected to the top of the scraper 201, and the fixing rods 202 are slidably connected to the moving plate 104 on the same side. A limiting plate 203 is fixedly connected to the top of the fixing rod 202. A spring 204 is sleeved on the outside of the fixing rod 202, and the two ends of the spring 204 are fixedly connected to the limiting plate 203 and the moving plate 104, respectively. Three sets of movable cavities are equidistantly arranged on the full-wavelength microplate reader body 1. A strip-shaped groove 205 is fixedly connected between the movable cavities and the detection chamber 2. A collection box 206 is slidably connected inside the movable cavities. The length of the scraper 201 matches the width of the detection chamber 2, which can ensure the thorough cleaning of the bottom of the inner cavity of the detection chamber 2.

[0035] The wiping assembly includes four sets of symmetrically fixed vertical rods 301 at the bottom of the microporous plate 3, with the vertical rods 301 slidably connected to the horizontal plate 105. A limit plate 302 is fixedly connected to the bottom end of each vertical rod 301. A spring 303 is sleeved on the outside of each vertical rod 301, with both ends of the spring 303 fixedly connected to the microporous plate 3 and the horizontal plate 105 respectively. Four sets of connecting seats 304 are symmetrically fixedly connected to the top of the horizontal plate 105, and the connecting seats 304 are slidably connected to the microporous plate 3. A one-way screw 305 is rotatably connected between two sets of connecting seats 304 on one side, and two sets of connecting seats 304 on the other side... A guide rod 306 is fixedly connected between the two parts. A movable plate 307 is externally threaded to the one-way screw 305 and is slidably connected to the guide rod 306. A wiping cotton 308 is provided at the bottom of the movable plate 307. Gears 309 are fixedly connected to both ends of the one-way screw 305 on the same axis. Two sets of racks 310 are symmetrically fixedly connected to the inner wall of the detection cavity 2 and are meshed with the gears 309 on the same side. The length of the movable plate 307 matches the width of the microporous plate 3, which can ensure the thorough cleaning of the surface of the microporous plate 3.

[0036] The implementation principle of a multifunctional microplate reader in this embodiment is as follows: First, the cover plate 4 is opened, then the motor 101 is started. The motor 101 drives the bidirectional lead screw 103 to rotate. Under the sliding engagement between the moving plate 104 and the guide rod 107, the two sets of moving plates 104 can move towards each other. Utilizing the hinged engagement between the moving plate 104, the horizontal plate 105, and the connecting rod 106, the two sets of moving plates 104 can push the horizontal plate 105 upward when moving towards each other, thereby promoting... The microplate 3 rises to the top opening of the detection chamber 2 to facilitate sample placement. Simultaneously, as the microplate 3 moves vertically, it also drives two sets of gears 309. The meshing between the gears 309 and the rack 310 drives the one-way lead screw 305 to rotate. Through the sliding engagement between the second moving plate 307 and the second guide rod 306, the second moving plate 307 moves horizontally, thereby enabling the wiping cotton 308 to clean the surface of the microplate 3. The glassware containing the sample is then placed on the microplate 3. Due to the increased weight and the sliding engagement between the vertical rod 301 and the horizontal plate 105, the microplate 3 descends and compresses the second spring 303. At this point, the wiping cotton 308 separates from the surface of the microplate 3. The wiping cotton 308 is then replaced. Afterward, the motor 101 is started, driving the bidirectional lead screw 103 to reverse. The microplate 3 then descends automatically. Because the wiping cotton 308 is separated from the surface of the microplate 3, it is not affected by the glassware during movement. The wiping cotton 308 has been replaced, so no dust or impurities will fall into the glassware. After the glassware containing the sample enters the detection chamber 2, the cover 4 is closed, and the full-wavelength microplate reader 1 is started through the control panel 5 to detect the sample. The full-wavelength microplate reader 1 supports accurate detection in the wavelength range of 200-1000nm, meeting the experimental requirements of colorimetry, turbidimetry, etc., and ensuring the accuracy of the results. The full-wavelength microplate reader 1 uses high-quality filters to ensure high wavelength accuracy such as ±0.With a 3nm wavelength and a large peak value, stray light interference can be effectively reduced. Finally, after the detection is completed, the cover plate 4 is opened and the motor 101 is restarted. When the two sets of moving plates 104 move towards or away from each other, they can also drive the scraper 201 below them to move together, thereby cleaning the bottom of the inner cavity of the detection chamber 2. The scraped sample will enter the collection box 206 through the strip groove 205, thus avoiding sample spillage and residue inside the full-wavelength microplate reader 1 and affecting its use. At the same time, the sliding cooperation between the fixed rod 202 and the moving plate 104 is used to spring... The spring 204's own rebound action ensures that the scraper 201 remains in contact with the bottom of the detection chamber 2, guaranteeing thorough cleaning of the bottom of the detection chamber 2. Once the glassware containing the sample is pushed out of the detection chamber 2, it can be directly removed. Due to the reduced weight and the rebound action of the spring 303, the microplate 3 automatically resets. The vibration generated during the reset process shakes off any residue in the microplate 3, improving the cleaning effect. Repeating the above operation allows for the testing of subsequent samples.

[0037] Example 2:

[0038] Based on Example 1, the further improvement of this embodiment is that the wiping cotton 308 is fixedly connected to the second movable plate 307 by adhesive bonding. The wiping cotton 308 and the second movable plate 307 are connected and fixed by adhesive bonding, which makes it easy to disassemble and replace the wiping cotton 308 at any time.

[0039] All components of this invention are general standard parts or parts known to those skilled in the art. Their structure and principles can be learned by those skilled in the art through technical manuals or conventional experimental methods. It is obvious to those skilled in the art that this invention is not limited to the details of the above exemplary embodiments, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects. The scope of this invention is defined by the appended claims rather than the foregoing description, and therefore all variations falling within the meaning and scope of equivalents of the claims are intended to be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0040] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A multifunctional microplate reader, characterized in that, The device includes a full-wavelength microplate reader body (1), which has a detection chamber (2) for detecting samples. The detection chamber (2) has a microplate (3) for placing samples inside. The full-wavelength microplate reader body (1) is rotatably connected to a cover plate (4) for sealing the detection chamber (2) via a rotating shaft. The full-wavelength microplate reader body (1) also has a control panel (5) for controlling the opening and closing of the device. The detection chamber (2) has a sample pick-and-place assembly, which has a cleaning assembly. The microplate (3) has a wiping assembly.

2. The multifunctional microplate reader as described in claim 1, characterized in that, The sample handling assembly includes a motor (101) fixedly connected to the full-wavelength microplate reader body (1). The rotor end of the motor (101) is coaxially fixedly connected to a connecting shaft (102), and the connecting shaft (102) is rotatably connected to the full-wavelength microplate reader body (1). The detection chamber (2) is rotatably connected to a bidirectional lead screw (103), and one end of the bidirectional lead screw (103) is coaxially fixedly connected to the connecting shaft (102). Both sides of the bidirectional lead screw (103) are threadedly connected to a moving plate (104). A horizontal plate (105) is movably arranged below the microplate (3). The horizontal plate (105) and the moving plate (104) are symmetrically hinged to two sets of connecting rods (106) through hinges. The detection chamber (2) is fixedly connected to a guide rod (107), and the guide rod (107) is simultaneously slidably connected to the two sets of moving plates (104).

3. The multifunctional microplate reader as described in claim 2, characterized in that, The cleaning assembly includes a scraper (201) disposed below the moving plate (104) and the scraper (201) is in contact with the bottom of the inner cavity of the detection chamber (2). Two sets of fixing rods (202) are symmetrically fixedly connected to the top of the scraper (201) and the fixing rods (202) are slidably connected to the moving plate (104) on the same side. A limiting plate (203) is fixedly connected to the top of the fixing rod (202). A spring (204) is sleeved on the outside of the fixing rod (202). Three sets of movable cavities are equidistantly opened on the full-wavelength microplate reader body (1). A strip groove (205) is fixedly connected between the movable cavity and the detection chamber (2). A collection box (206) is slidably connected inside the movable cavity.

4. The multifunctional microplate reader as described in claim 3, characterized in that, The wiping assembly includes four sets of vertical rods (301) symmetrically fixedly connected to the bottom of the microporous plate (3), and the vertical rods (301) are slidably connected to the horizontal plate (105). The bottom end of the vertical rod (301) is fixedly connected to a limiting plate (302). A spring (303) is sleeved on the outside of the vertical rod (301). The top of the horizontal plate (105) is symmetrically fixedly connected to four sets of connecting seats (304), and the connecting seats (304) are slidably connected to the microporous plate (3). A one-way lead screw (305) is rotatably connected between two sets of connecting seats (304) on one side, and on the other side... A guide rod (306) is fixedly connected between the two sets of connecting seats (304) on the side. The external thread of the one-way screw (305) is connected to a moving plate (307), and the moving plate (307) is slidably connected to the guide rod (306). A wiping cotton (308) is provided at the bottom of the moving plate (307). Both ends of the one-way screw (305) are coaxially fixedly connected to gears (309). Two sets of racks (310) are symmetrically fixedly connected to the inner wall of the detection cavity (2), and the racks (310) are meshed with the gears (309) on the same side.

5. The multifunctional microplate reader as described in claim 4, characterized in that, The horizontal plate (105) is provided with rectangular through holes (108) evenly distributed, and the rectangular through holes (108) correspond to the holes on the microporous plate (3).

6. The multifunctional microplate reader as described in claim 5, characterized in that, The length of the scraper (201) is matched with the width of the detection cavity (2).

7. The multifunctional microplate reader as described in claim 6, characterized in that, The length of the movable plate 2 (307) is matched with the width of the microporous plate (3).

8. The multifunctional microplate reader as described in claim 7, characterized in that, The wiping cotton (308) is fixedly connected to the second movable plate (307) by adhesive bonding.

9. The multifunctional microplate reader as described in claim 8, characterized in that, The two ends of the spring (204) are fixedly connected to the limiting plate (203) and the moving plate (104) respectively.

10. The multifunctional microplate reader as described in claim 9, characterized in that, The two ends of the second spring (303) are fixedly connected to the micro-perforated plate (3) and the horizontal plate (105), respectively.

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