Essential oil for releasing fascia by stone needle as well as preparation method and application of essential oil

Abstract

The invention belongs to the technical field of health care, and particularly relates to essential oil for releasing fascia through a stone needle and a preparation method and application of the essential oil. According to the essential oil, sunflower seed oil and peony seed oil serve as base oil, turmeric root oil, ginger root oil and wintergreen leaf oil are matched, active ingredients such as phellinus igniarius polysaccharide extract, polyphyllin extract, borneol and tocopherol are added, the formula is scientific, and the synergistic effect is obvious. The preparation method relates to the processes of multi-enzyme synergistic enzymolysis, supercritical CO2 extraction, molecular distillation, efficient extraction, purification and the like, and high-activity and high-purity key components can be obtained. The essential oil disclosed by the invention has good permeability, anti-inflammatory property, repairability and safety, can remarkably improve the smoothness and comfort of fascia release of the stone needle knife, and is suitable for multiple physiotherapy scenes such as fascia rehabilitation, soft tissue repair and pain relief.

Description

Technical Field

[0001] This invention belongs to the field of health care technology, specifically relating to an essential oil for releasing fascia with Bian stone knife, its preparation method, and its application. Background Technology

[0002] Fascia is an important connective tissue that covers and connects the body's muscles, bones, and other structures. Its health directly affects the body's coordination and soft tissue function. Recent studies have found that fascial nodules and adhesions are common causes of chronic pain and limited joint mobility, and in severe cases, they can even affect patients' quality of life. Currently used methods for fascial-related diseases, such as massage, acupuncture, scraping, and physical rehabilitation, can alleviate some discomfort, but most are limited to superficial tissues and lack effective intervention for deep fascial lesions. Furthermore, the effects are short-lived and have a high recurrence rate. Minimally invasive release surgery can directly address adhesions, but it carries risks of trauma and slow recovery, making it unsuitable for most patients. Drug treatment has drawbacks such as side effects and dependence, thus failing to become an ideal solution for chronic fascial diseases.

[0003] Against this backdrop, the Bian stone knife technique for releasing fascia has received increasing attention. This technique integrates anatomical principles with traditional Bian stone therapy, using a specialized Bian stone knife to precisely separate fascial nodules and adhesions through cutting, rotating, and twisting methods, achieving deep release. Its advantages include rapid effectiveness, minimal damage, and a short recovery period, demonstrating unique value in the rehabilitation of some chronic pain and functional impairments. However, in practice, fascial tissue is dense and lesions are often quite hard. Relying solely on mechanical methods often faces challenges such as high resistance, incomplete release, and significant local pain, which not only affects the patient's experience but also limits the sustainability and improvement of therapeutic effects.

[0004] The use of auxiliary essential oils can, to some extent, reduce friction between the Bian knife and the skin and fascia, improving the smoothness of the release process. However, most commercially available essential oils are designed for superficial massage and lack the ability to deeply penetrate and repair the fascia layer, thus failing to adequately support core issues such as softening fascial nodules, relieving inflammation, and promoting tissue recovery. There is currently no professional essential oil formula on the market specifically designed for Bian knife fascia release, balancing permeability and bioactivity. Developing an essential oil that can synergize with Bian knife manipulation and improve the efficiency and comfort of fascia release has become a pressing issue in the field of fascial rehabilitation. Summary of the Invention

[0005] The purpose of this invention is to provide an essential oil for fascia release using Bian knife therapy, its preparation method, and its application. By scientifically combining various ingredients with good permeability and bioactivity, the essential oil can effectively soften fascial nodules, reduce friction and discomfort during operation, promote the repair of the fascial layer and relieve inflammation, thereby improving the efficiency and comfort of fascia release using Bian knife therapy and meeting the actual needs in the clinical fascial rehabilitation process.

[0006] To achieve the above objectives, the present invention provides the following technical solution:

[0007] The first aspect of this invention provides an essential oil for releasing fascia with Bian stone therapy, the raw materials of which, by weight, include: 30-60 parts of Helianthus Annuus Seed Oil, 5-15 parts of Curcuma Longa Root Oil, 3-10 parts of Zingiber Officinale Root Oil, 5-15 parts of Paeonia Suffruticosa Seed Oil, 1-5 parts of Phellinus Linteus Polysaccharides Extract, 0.2-1.5 parts of Paris Polyphylla Saponins Extract, 1-5 parts of Gaultheria Procumbens Leaf Oil, 0.1-1 part of borneol, and 0.5-2 parts of tocopherol.

[0008] Furthermore, the turmeric root oil is prepared according to the following method: (1) After drying the turmeric root, pulverize it and sieve it to obtain turmeric root powder. Add water to adjust the water weight ratio to 80%-90% to obtain the enzymatic hydrolysis substrate. Pour nitrogen gas, add a compound enzyme for enzymatic hydrolysis, inactivate the enzyme, filter and dry to a moisture content of ≤10% to obtain the enzymatic hydrolysis product. (2) The enzymatic hydrolysis products were subjected to supercritical carbon dioxide fractional extraction to obtain the first and second products. (3) Molecular distillation was performed on the second product to obtain a purified product; (4) Combine the first product and the purified product in a certain proportion to obtain the final product.

[0009] Furthermore, in step (1), the complex enzyme includes cellulase, pectinase and β-glucosidase.

[0010] Furthermore, the amounts of cellulase, pectinase, and β-glucosidase added are 150-250 U / g of enzymatic hydrolysis substrate, 40-60 U / g of enzymatic hydrolysis substrate, and 20-40 U / g of enzymatic hydrolysis substrate, respectively.

[0011] Furthermore, the specific conditions for the enzymatic hydrolysis are: temperature of 45-50℃, pH of 4.8-5.2, rotation speed of 150-200 rpm, and hydrolysis time of 3-4 hours.

[0012] Furthermore, the specific conditions for enzyme inactivation are: temperature of 85-90℃ and time of 8-10 min.

[0013] Furthermore, in step (2), the supercritical carbon dioxide fractional extraction includes a first stage of low-pressure extraction and a second stage of high-pressure enrichment.

[0014] Furthermore, the conditions for the first stage of low-pressure extraction are: pressure of 25-28 MPa, temperature of 42-50℃, CO2 flow rate of 2-3 L / min, extraction time of 1-1.5 h, and the first product is collected through a primary separator.

[0015] Furthermore, the specific parameters of the primary separator are: pressure of 6-10 MPa and temperature of 20-30℃.

[0016] Furthermore, the conditions for the second stage of high-pressure enrichment are: pressure 38-40 MPa, temperature 55-65℃, CO2 flow rate 1.5-2 L / min, entrainer flow rate 5%-8% of CO2 flow rate, extraction time 2-2.5 h, and the second stage product is collected through a secondary separator.

[0017] Furthermore, the entrainer is an aqueous solution of ethanol with a mass fraction of 95%.

[0018] Furthermore, the specific parameters of the secondary separator are: pressure of 3-5 MPa and temperature of 35-45℃.

[0019] Furthermore, in step (3), the specific conditions for molecular distillation are: feed rate of 0.8-1.2 mL / min, evaporation temperature of 180-200℃, system vacuum of 0.1-0.5 Pa, and condensation temperature of 43-47℃.

[0020] Furthermore, in step (4), the mass ratio of the first product to the purified product is (7-9):(1-3).

[0021] The turmeric root oil prepared using this invention significantly increases the content of curcuminoid compounds, making the final essential oil more effective in softening fascia induration and relieving inflammation. First, turmeric root is synergistically hydrolyzed using a combination of enzymes (cellulase, pectinase, and β-glucosidase), effectively disrupting the cell wall structure and promoting the conversion of curcumin precursors, thus significantly increasing curcumin release. Subsequently, supercritical carbon dioxide fractional extraction technology is employed. Low-pressure conditions help retain the active ingredients in the turmeric root oil, while the high-pressure stage, combined with an entrainer, enables targeted enrichment of curcumin, avoiding the degradation of active substances caused by traditional high-temperature treatment. Next, the curcumin fraction is purified by molecular distillation to obtain a high-purity curcumin product. Finally, the high-purity curcumin is blended with whole essential oil in a specific ratio, not only retaining the original aroma and volatile anti-inflammatory and penetration-promoting components of turmeric root but also significantly increasing the content of anti-inflammatory and antioxidant components. The turmeric root oil prepared through the above optimization, in synergy with other components such as ginger root oil in the formula, can further enhance the rapid softening and repair capabilities of fascial nodules. Furthermore, the process of this invention can effectively remove potentially irritating impurities, reducing skin irritation and making it more suitable for applications such as large-area and frequent massage therapy.

[0022] Furthermore, the Phellinus linteus polysaccharide extract is prepared according to the following method: (1) The dried Phellinus linteus fruiting body is crushed and sieved to obtain Phellinus linteus powder. The Phellinus linteus powder is mixed with anhydrous ethanol and degreased to obtain degreased Phellinus linteus powder. (2) Mix defatted Phellinus linteus powder with water at a ratio of 1:15-1:20, soak at 40-45℃ for 30-60 minutes to obtain Phellinus linteus aqueous suspension, add cellulase, pectinase and chitosanase to Phellinus linteus aqueous suspension in sequence, inactivate enzymes after enzymatic hydrolysis, filter and collect filtrate to obtain polysaccharide extract. (3) Concentrate the polysaccharide extract to 20-30% of its original volume, add anhydrous ethanol, let stand, filter, collect the precipitate, wash the precipitate, and obtain crude polysaccharide precipitate; (4) Add water to the crude polysaccharide precipitate to obtain a crude polysaccharide solution. Then add trichloroacetic acid to the crude polysaccharide solution, let it stand in an ice bath, add n-butanol, centrifuge to remove solid impurities, and obtain a deproteinized polysaccharide solution. (5) The polysaccharide solution after deproteinization was dialyzed, and the dialyzed polysaccharide solution was freeze-dried to obtain the final Phellinus linteus polysaccharide extract.

[0023] Furthermore, the amounts of cellulase, pectinase, and chitosanase added are 200-300 U / g of Phellinus linteus aqueous suspension, 30-50 U / g of Phellinus linteus aqueous suspension, and 50-80 U / g of Phellinus linteus aqueous suspension, respectively.

[0024] Furthermore, the enzymatic hydrolysis conditions are: pH value of 4.8-5.2, enzymatic hydrolysis temperature of 45-50℃, and enzymatic hydrolysis time of 3-5 hours.

[0025] Further, in step (3), the amount of trichloroacetic acid added is 4-6% of the mass of the crude polysaccharide solution, and the amount of n-butanol added is 1-3% of the mass of the crude polysaccharide solution.

[0026] This invention employs a synergistic enzymatic hydrolysis method using three enzymes—cellulase, pectinase, and chitosanase—in the extraction of polysaccharides from *Phellinus linteus*. The cell wall structure of *Phellinus linteus* fruiting bodies is complex, containing not only abundant cellulose but also pectin, chitosan, and other polysaccharides and polysaccharide complexes. Using a single enzyme is insufficient to fully disrupt the cell wall and release intracellular active polysaccharides. Cellulase effectively degrades the cell wall backbone, improving the release efficiency of cell contents; pectinase targets pectin components, further depolymerizing the intercellular matrix and enhancing cell disintegration and polysaccharide diffusion; chitosanase specifically decomposes the unique chitosan component in the fungal cell wall, helping to further overcome the protective barrier of the *Phellinus linteus* fungal wall. The synergistic effect of these three enzymes achieves multi-target disruption of the *Phellinus linteus* fruiting body cell wall, significantly increasing the polysaccharide dissolution rate, while operating under mild conditions to protect the structural integrity and biological activity of the polysaccharide molecules. Compared to traditional single-enzyme or hot water methods, this method significantly improves the polysaccharide yield and quality, laying the foundation for obtaining high-purity polysaccharide products.

[0027] Furthermore, the Paris polyphylla saponin extract is prepared according to the following method: (1) Crush the dried Paris rhizomes and sieve them to obtain Paris powder; (2) Mix Paris polyphylla powder with anhydrous ethanol, then add 1-butyl-3-methylimidazolium tetrafluoroborate, extract the mixture by ultrasonication, filter after extraction, collect the filtrate, and concentrate the filtrate. (3) After diluting the concentrated filtrate, pass it through a D101 macroporous adsorption resin column, wash off impurities with water, and then elute with ethanol to collect the eluent containing saponins. (4) The eluent was concentrated and freeze-dried to obtain Paris polyphylla saponin extract.

[0028] Further, in step (2), the mass ratio of Paris polyphylla powder to anhydrous ethanol is 1:(10-15), and the mass of 1-butyl-3-methylimidazolium tetrafluoroborate is 1-2% of the mass of Paris polyphylla powder.

[0029] Furthermore, the ultrasonic extraction temperature in step (2) is 45-55℃, the ultrasonic power is 300-500W, and the extraction time is 40-60 minutes.

[0030] This invention introduces 1-butyl-3-methylimidazolium tetrafluoroborate as an auxiliary extraction agent in the extraction process of saponins from Paris polyphylla. Traditional solvent extraction methods using ethanol and methanol often face problems such as incomplete cell wall disruption and insufficient dissolution of active ingredients. Ionic liquids, due to their unique polarity, good chemical stability, and low volatility, can effectively penetrate cell structures and form good miscibility with various organic molecules inside and outside the cells, significantly enhancing the dissolution and extraction capabilities of large polar molecules such as saponins. Simultaneously, ionic liquids can synergistically work with ultrasound, utilizing ultrasonic cavitation to further accelerate cell wall disruption and the diffusion and transfer of active ingredients, greatly improving extraction efficiency and saponin purity.

[0031] The second aspect of this invention provides a method for preparing the aforementioned essential oil for fascia release using Bian stone therapy, comprising the following steps: (1) Mix sunflower seed oil and peony seed oil and stir to obtain a base oil mixture; (2) Add turmeric root oil, ginger root oil and holly leaf oil to the above base oil mixture, and continue stirring to obtain the mixed oil solution; (3) Take 10-15 parts of the mixed oil solution, add the Phellinus linteus polysaccharide extract dissolved in deionized water, stir and shear to obtain Phellinus linteus polysaccharide dispersion; take another 10-15 parts of the mixed oil solution, add the Paris polyphylla saponin extract dissolved in deionized water, stir and shear to form Paris polyphylla saponin dispersion; add the Phellinus linteus polysaccharide dispersion and Paris polyphylla saponin dispersion to the remaining mixed oil solution, stir to obtain the active ingredient mixed oil solution; (4) Add borneol and tocopherol to the above-mentioned active ingredient mixture, stir, filter, and obtain the essential oil for fascia release by Bian knife.

[0032] Furthermore, in step (3), an emulsifier may be added to the Phellinus linteus polysaccharide extract dissolved in deionized water and the Paris polyphylla saponin extract dissolved in deionized water to enhance the dispersion stability of the extract.

[0033] Furthermore, the emulsifier is selected from lecithin, sodium capryloyl lactylate, polysorbate-80, Tween-60, or a combination thereof.

[0034] The third aspect of this invention provides the application of the above-mentioned essential oil for fascia release using Bian knife in the preparation of fascia rehabilitation products, muscle fascia release products, chronic soft tissue injury repair products, pain relief products, physical therapy massage products, and products that assist in Bian knife fascia release operations.

[0035] The essential oils provided by this invention for fascia release via Bian stone therapy are scientifically formulated with synergistic components, possessing excellent permeability, anti-inflammatory properties, restorative effects, and safety. They effectively improve the smoothness of fascia release procedures and enhance clinical outcomes. Sunflower seed oil and peony seed oil serve as base oils, offering not only good skin affinity and permeability but also a gentle carrier environment for the essential oil system, ensuring stable release of active ingredients. Turmeric root oil and ginger root oil, within appropriate dosage ranges, effectively soften fascial nodules, alleviate inflammation, and enhance tissue repair capabilities due to their significant anti-inflammatory, antioxidant, and blood circulation-promoting effects. Wintergreen leaf oil, with its unique analgesic and anti-swelling properties, further enhances comfort during the procedure and reduces patient discomfort.

[0036] The formula is specially fortified with self-made highly active Phellinus linteus polysaccharide extract and Paris polyphylla saponin extract, both of which have a synergistic effect in promoting fascial tissue repair, anti-fibrosis, and anti-inflammation. Phellinus linteus polysaccharide extract can efficiently release active polysaccharides, not only aiding in the regeneration and repair of the fascial layer but also reducing chronic inflammatory responses. Paris polyphylla saponin extract is rich in high-purity saponin components, effectively inhibiting fibrosis and pain during fascial pathology. Borneol in the formula promotes the penetration of other active ingredients, enhancing the efficacy to reach the fascial layer and providing a cooling sensation, improving the user experience. Tocopherol, as a natural antioxidant, not only helps extend the shelf life of essential oil products but also synergistically protects unsaturated fatty acids and active substances in essential oils from oxidative loss.

[0037] The dosage range of each component in this invention has been optimized through systematic experiments and scientific proportioning. This ensures that each component can fully exert its efficacy while avoiding mutual interference and irritation between components, so that the essential oil as a whole can exhibit multiple advantages such as high-efficiency softening, anti-inflammatory, analgesic, promoting repair and being safe and skin-friendly.

[0038] During the fascia release procedure using a Bian stone knife, the following method is preferred: First, the operator should adjust the knife's entry angle according to the target fascial area, ideally between 0 and 30 degrees, to ensure precise application to the tissue layers. The release procedure typically involves applying force in a "light-light-heavy" sequence, each lasting 1 to 3 seconds, to achieve gradual separation of tissue layers. Specifically, the first and second steps are initially completed with lighter force, focusing on probing and locating fascial nodules, followed by heavier force to release and separate the nodules. During the release process, the operator needs to flexibly adjust the knife technique, using a "clockwise rubbing, pressing, and pulling" motion to push the knife head clockwise from bottom to top (6 o'clock to 12 o'clock), and then using a "clockwise pressing and pulling" motion to slide the knife tail clockwise from top to bottom (12 o'clock to 6 o'clock) to fully separate fascial adhesions and nodules. Each layer of action should be performed sequentially, compacting the transition layer, softening the separation layer, and opening the nodular layer to gradually soften and release the fascia. Finally, a gentle traction technique should be used to gently pull along the fascial fibers to enhance the effectiveness of fascial release and improve patient comfort.

[0039] During the Bian knife fascia release procedure, to further ensure the accuracy and safety of the operation, it is necessary to pay attention to the delicate control of movements and the judgment of the layered response of the muscle and fascia. Specifically, whether the layered release has been achieved can be judged by the friction felt during actual operation: when the Bian knife is gently pushed at the target layer, if obvious frictional resistance is felt, it indicates that the fascia layer has been effectively separated; conversely, if there is no resistance, it indicates that the release is not yet complete, and the operating angle and force need to be adjusted further. In addition, special attention should be paid to the relationship between the blade and the skin during operation. Ideally, the blade should be slightly upturned, which can prevent damage to the epidermis and help to accurately control the depth of action.

[0040] Throughout the entire process of releasing the layers, the force applied should follow the principle of "light-light-heavy." The first two gentle movements aim to relax the relevant soft tissues, while the third movement should apply appropriate pressure to break through nodules or adhesions. During each pause, initiation, and termination of the procedure, the wrist must be kept stable, avoiding large swings to prevent the operation from becoming mere superficial friction or scraping. When actually separating the fascia, it is advisable to combine this with combined movements such as "rubbing, pressing, and pulling" and "pressing and pulling" to ensure that the blade moves in synergy with the fascia layer.

[0041] During the procedure, appropriate scalpel tips and techniques should be selected based on the type of fascial nodules or adhesions. For example, harder nodules such as fibrosis and leatheriness can be treated with point pressure from a conical scalpel tip and a blunt scalpel for dispersing. For cord-like, calcified, or spastic lesions, treatment should be carried out in stages and at different points to avoid damaging healthy tissue with excessive force in a single session. For severe fascial lesions, multiple, staged releases are necessary, combined with overall functional assessment and balance adjustment of the muscle group, to gradually restore tissue elasticity and functional integrity.

[0042] It is important to note that Bian knife myofascial release is not solely aimed at "opening joints," but also emphasizes the post-treatment balance repair of muscles and fascia. Some patients may experience pain or discomfort in the short term after the procedure; this is a normal reaction to muscle repair and balance reconstruction, and generally subsides gradually during the consolidation phase. For myofascial nodules of varying degrees of injury, a personalized treatment frequency and rehabilitation cycle should be developed. Mild cases can be treated once a week, moderate cases once every two weeks, and severe cases can be extended to once a month. The treatment plan should be dynamically adjusted based on a comprehensive assessment of the patient's self-healing ability and recovery response to achieve the best rehabilitation results.

[0043] Compared with the prior art, the advantages and beneficial effects of the present invention are as follows: Compared with existing technologies, the essential oil for fascia release using Bian stone therapy provided by this invention has the following significant beneficial effects: First, the essential oil formula scientifically combines base oil with various functional essential oils and plant active extracts, ensuring both good skin penetration and lubrication while significantly enhancing the softening ability of fascial nodules. Experimental results show that the essential oil of this invention is superior to the comparative formula in terms of lubrication, penetration, nodule softening, and anti-inflammatory properties, effectively reducing operational resistance and improving patient comfort. Second, the introduction of turmeric root oil and various innovative extracts such as Paris polyphylla saponins and Phellinus linteus polysaccharides gives the product strong anti-inflammatory, anti-fibrotic, and fascia tissue repair-promoting effects, accelerating fascia function recovery and reducing local inflammation and pain. Furthermore, the unique extraction process of this invention ensures high purity and high activity of key components, and the finished essential oil is safe and mild, suitable for long-term and frequent physiotherapy use. Detailed Implementation

[0044] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0045] Unless otherwise specified, all raw materials used in the embodiments are commercially available products. The following sources are for illustrative purposes only: Sunflower seed oil was purchased from Gansu Jingye Agricultural Technology Co., Ltd. Ginger root oil was purchased from Jiangxi Baolin Natural Fragrance Co., Ltd. Peony seed oil was purchased from Guangzhou Fujie Biotechnology Co., Ltd. Holly leaf oil was purchased from Shaanxi Zhengmao Biotechnology Co., Ltd. Borneol was purchased from Zhengzhou Kangyuan Chemical Products Co., Ltd. Tocopherol was purchased from Guangzhou Nuoran Biotechnology Co., Ltd. Cellulase (approximately 100,000 U / g), pectinase (approximately 30,000 U / g), β-glucosidase (approximately 2,000 U / g), and chitosanase (approximately 8,000 U / g) were all sourced from Shanghai Huashang Xiangyang Biotechnology Co., Ltd.

[0046] Example 1 This embodiment provides an essential oil for releasing fascia with Bian stone knife. The raw materials for its preparation include, by weight, 50 parts sunflower seed oil, 10 parts turmeric root oil, 6 parts ginger root oil, 10 parts peony seed oil, 3 parts Phellinus linteus polysaccharide extract, 1 part Paris polyphylla saponin extract, 3 parts holly leaf oil, 0.5 parts borneol, and 1 part tocopherol.

[0047] Turmeric root oil is prepared according to the following method: (1) Take 1000g of dried turmeric root, crush it and pass it through an 80-mesh sieve to obtain turmeric root powder. Add deionized water to the turmeric root powder to adjust the water weight ratio to 85% to obtain the enzymatic hydrolysis substrate. After passing nitrogen gas for 30 minutes, add cellulase (200U / g enzymatic hydrolysis substrate), pectinase (50U / g enzymatic hydrolysis substrate), and β-glucosidase (30U / g enzymatic hydrolysis substrate) in sequence. Adjust the pH to 5.0, maintain the temperature at 47℃, stir at 180rpm, and enzymatically hydrolyze for 3.5 hours. After the enzymatic hydrolysis is completed, heat the mixture to 87℃ and maintain it for 10 minutes to inactivate the enzyme. After inactivation of the enzyme, filter it with a vacuum filter and collect the filter residue. Dry the filter residue at 50℃ to a moisture content of 8.93% to obtain the enzymatic hydrolysis product.

[0048] (2) The obtained enzymatic hydrolysis product was loaded into the extraction vessel of the supercritical CO2 extraction equipment. First, low-pressure extraction was performed. The pressure was set at 27 MPa, the temperature at 45 °C, and the CO2 flow rate at 2.5 L / min. The extraction was carried out for 1.2 hours. The product was collected through the first-stage separator (pressure at 8 MPa, temperature at 25 °C) to obtain the first-stage product. Then, the extraction pressure was increased to 39 MPa, the temperature at 60 °C, and the CO2 flow rate at 1.7 L / min. 95% ethanol aqueous solution was added as an entrainer (the flow rate was 6% of the CO2 flow rate). The extraction was carried out for 2.2 hours. The product was collected through the second-stage separator (pressure at 4 MPa, temperature at 40 °C) to obtain the second-stage product.

[0049] (3) The product of the second stage above is fed into a molecular distillation apparatus, with the feed rate set to 1.0 mL / min, the evaporation temperature to 190℃, the system vacuum to 0.3 Pa, and the condensation temperature to 45℃. Molecular distillation is then performed to collect the purified product.

[0050] (4) Mix the first product and the purified product at a mass ratio of 8:2 and stir evenly to obtain turmeric root oil.

[0051] Phellinus linteus polysaccharide extract was prepared according to the following method: (1) Weigh 1000g of dried Phellinus linteus fruiting body, crush it with a high-speed pulverizer and pass it through an 80-mesh sieve to obtain Phellinus linteus powder. Mix Phellinus linteus powder with 4000ml of anhydrous ethanol and stir magnetically at room temperature for 2 hours to remove fat-soluble impurities. After defatting, use vacuum filtration to filter, discard the ethanol filtrate, rinse the filter residue twice with 500ml of purified water, spread it out and let it stand until the ethanol basically evaporates to obtain defatted Phellinus linteus powder.

[0052] (2) Mix defatted Phellinus linteus powder with purified water at a mass ratio of 1:17, stir and soak at 40°C for 45 minutes to obtain Phellinus linteus aqueous suspension, then add cellulase (250 U / g Phellinus linteus aqueous suspension), pectinase (40 U / g Phellinus linteus aqueous suspension) and chitosanase (60 U / g Phellinus linteus aqueous suspension) in sequence, adjust the pH to 5.0, maintain the enzymatic hydrolysis temperature at 47°C, and stir for 4 hours for enzymatic hydrolysis; after enzymatic hydrolysis, heat the mixture to 90°C and keep it at 10 minutes to inactivate the enzyme; after enzyme inactivation, filter with a vacuum filter and collect the filtrate to obtain polysaccharide extract.

[0053] (3) The polysaccharide extract was concentrated under reduced pressure at 60°C to 25% of its original volume, cooled to room temperature, and anhydrous ethanol was slowly added to the cooled concentrate to make the ethanol volume fraction of the system reach 85%. The mixture was stirred thoroughly and allowed to stand at 4°C for 12 hours. After standing, the precipitate was collected by vacuum filtration and washed twice with anhydrous ethanol to obtain crude polysaccharide precipitate.

[0054] (4) Dissolve the crude polysaccharide precipitate in 2000 ml of purified water to obtain a crude polysaccharide solution. Add trichloroacetic acid to the crude polysaccharide solution, the amount of trichloroacetic acid added is 5% of the mass of the crude polysaccharide solution. After stirring and dissolving, place it in an ice bath and let it stand for 40 minutes. Then add n-butanol, the amount of n-butanol added is 1.5% of the mass of the crude polysaccharide solution. Mix gently and continue to stand in an ice bath for 15 minutes. Then use refrigerated centrifugation (5000 rpm, 15 minutes) to collect the supernatant, which is the polysaccharide solution after deproteinization.

[0055] (5) The deproteinized polysaccharide solution was placed into a dialysis bag with a molecular weight cutoff of 3500 Da and dialyzed in flowing purified water for 36 hours. The water was changed every 8 hours for a total of 4 times. After dialysis, the polysaccharide solution was taken out and freeze-dried for 48 hours to obtain Phellinus linteus polysaccharide extract powder.

[0056] Paris polyphylla saponin extract was prepared according to the following method: (1) Weigh 1000 grams of dried Paris rhizome, remove surface impurities, cut into pieces and crush with a high-speed pulverizer, and pass through an 80-mesh sieve to obtain fine Paris rhizome powder.

[0057] (2) Mix 1000g of finely powdered Paris polyphylla with 10000ml of anhydrous ethanol and stir until homogeneous. Then add 1-butyl-3-methylimidazolium tetrafluoroborate to the mixture at 2% of the mass of the finely powdered Paris polyphylla. Transfer the mixture to a stainless steel ultrasonic extraction vessel and perform ultrasonic extraction at 45℃ with an ultrasonic power of 400W for 50 minutes. After ultrasonic extraction, cool to room temperature, filter with a vacuum filter, collect the filtrate, and concentrate the filtrate under reduced pressure at 50℃ to 1 / 4 of the original filtrate volume to obtain a concentrated solution.

[0058] (3) Dilute the concentrate obtained in step (2) with purified water to 5000 ml. Take 2 liters of pretreated D101 macroporous adsorption resin and pack it into a glass chromatography column. Add the diluted concentrate evenly and slowly to the resin column, controlling the flow rate to 1-2 ml / min, so that the raw material solution can fully contact the resin. After all the samples are loaded, use purified water to elute the resin column at a flow rate of 1000 ml / hour. Collect the eluent until the eluent is colorless.

[0059] After discarding the purified water eluent, 50% ethanol solution was used as the eluent, and the resin column was continued to be eluted at a flow rate of 1000 ml per hour. All ethanol eluent was collected, combined, and placed in a rotary evaporator. The eluent was concentrated under reduced pressure at 50°C to a viscous state to obtain a concentrated eluent rich in Paris saponins.

[0060] (4) The above concentrated eluent was dispensed into freeze-drying bottles, placed in a freeze dryer, and freeze-dried at -50°C for 48 hours to obtain Paris polyphylla saponin extract powder.

[0061] The essential oils used for releasing fascia with Bian stone therapy are prepared according to the following method: (1) Weigh sunflower seed oil and peony seed oil, place them in a clean stainless steel mixing container, and use a mechanical stirrer to stir at a speed of 300 revolutions per minute for 10 minutes to fully mix and obtain a base oil mixture.

[0062] (2) While stirring, add turmeric root oil, ginger root oil and wintergreen leaf oil to the above base oil mixture, and continue stirring for 10 minutes to evenly disperse each essential oil in the base oil to obtain a uniform mixed oil solution.

[0063] (3) After ultrafine grinding, 3 parts of Phellinus linteus polysaccharide extract were added to 8 parts of deionized water and stirred at 120 rpm for 20 minutes to dissolve the Phellinus linteus polysaccharide extract. Then, 0.15 parts of lecithin were added as an emulsifier and stirred at 150 rpm for 20 minutes at 65°C to obtain a mixture. Then, it was slowly poured into 15 parts of mixed oil and placed in a high-speed shear disperser and sheared at 10,000 rpm for 15 minutes to obtain Phellinus linteus polysaccharide dispersion. Add 1 part of Paris polyphylla saponin extract to 3 parts of deionized water and stir at 120 rpm to dissolve the Paris polyphylla saponin extract. Then add 0.05 parts of lecithin and continue stirring at 150 rpm for 20 minutes at 60°C to obtain a mixture. Then slowly pour it into 10 parts of mixed oil and place it in a high-speed shear disperser to shear at 10,000 rpm for 15 minutes to obtain Paris polyphylla saponin dispersion. The above-mentioned Phellinus linteus polysaccharide dispersion and Paris polyphylla saponin dispersion were slowly poured into the remaining main mixed oil in sequence, and sheared at 10,000 rpm for 20 minutes to obtain the active ingredient mixed oil.

[0064] (4) Weigh out borneol powder and tocopherol, and add them to the above active ingredient mixture in sequence. Stir at 300 rpm for 10 minutes until the borneol is completely dissolved. Filter the final mixture through a 200-mesh stainless steel filter to remove particles or insoluble matter. Collect the filtrate to obtain the finished essential oil.

[0065] Example 2 This embodiment provides an essential oil for releasing fascia with Bian stone knife, which differs from Embodiment 1 in that the raw materials for preparing the essential oil, by weight, include: 40 parts sunflower seed oil, 12 parts turmeric root oil, 4 parts ginger root oil, 15 parts peony seed oil, 2 parts Phellinus linteus polysaccharide extract, 1.3 parts Paris polyphylla saponin extract, 4 parts holly leaf oil, 0.5 parts borneol, and 1 part tocopherol.

[0066] Comparative Example 1 This comparative example provides an essential oil for releasing fascia with Bian stone knife, which differs from Example 1 in that the raw materials for preparing the essential oil, by weight, include: 25 parts sunflower seed oil, 4 parts turmeric root oil, 15 parts ginger root oil, 40 parts peony seed oil, 2 parts Phellinus linteus polysaccharide extract, 1.3 parts Paris polyphylla saponin extract, 4 parts holly leaf oil, 0.5 parts borneol, and 1 part tocopherol.

[0067] Comparative Example 2 This comparative example provides an essential oil for releasing fascia with Bian stone knife, which differs from Example 1 in that the raw materials for preparing the essential oil, by weight, include: 25 parts sunflower seed oil, 4 parts turmeric root oil, 15 parts ginger root oil, 40 parts peony seed oil, 0.5 parts Phellinus linteus polysaccharide extract, 3.5 parts Paris polyphylla saponin extract, 4 parts holly leaf oil, 0.5 parts borneol, and 1 part tocopherol.

[0068] Comparative Example 3 This comparative example provides an essential oil for releasing fascia with Bian stone knife, which differs from Example 1 in that the turmeric root oil is prepared according to the following method: After being washed and dried, turmeric root is crushed and passed through a 40-mesh sieve to obtain turmeric root powder. The turmeric root powder and water are thoroughly mixed at a ratio of 5 ml of water per gram of turmeric root powder, and soaked at room temperature for 30 minutes. Then, it is heated to boiling and distilled for 4 hours until no more oil droplets are separated from the condensate. The steam is condensed by circulating cold water at 20°C, and the oil-water mixture is collected. After the mixture is allowed to stand and separate into layers for 2 hours, the essential oil in the upper layer is separated. Anhydrous sodium sulfate is added to the obtained essential oil, and it is stirred thoroughly to absorb water for 24 hours. Finally, the essential oil is separated, which is the finished turmeric root oil.

[0069] Comparative Example 4 This comparative example provides an essential oil for releasing fascia with Bian knife therapy, which differs from Example 1 in that: The Phellinus linteus polysaccharide extract was prepared according to the following method: (1) Weigh 1000g of dried Phellinus linteus fruiting body, crush it with a high-speed pulverizer and then pass it through an 80-mesh sieve to obtain Phellinus linteus powder.

[0070] (2) Add 1000g of Phellinus linteus powder and 20000ml of purified water to a stainless steel reaction vessel, heat to boiling, and maintain water bath reflux extraction for 2 hours. After extraction, filter with multiple layers of gauze while hot, collect the filtrate, add 10000ml of purified water to the filter residue, repeat the above operation and extract for another 1 hour, and combine the two extraction filtrates.

[0071] (3) The combined extracts were concentrated to 1 / 4 of the original volume under reduced pressure at 60°C using a rotary evaporator. After cooling to room temperature, anhydrous ethanol was slowly added to make the volume fraction of ethanol in the mixture reach 80%. After stirring and mixing thoroughly, the mixture was placed in a refrigerator at 4°C for 12 hours to promote polysaccharide precipitation.

[0072] (4) After standing, the precipitate was separated by a refrigerated centrifuge, the supernatant was discarded, the precipitate was washed twice with anhydrous ethanol, and the precipitate was dried in a vacuum drying oven at 60°C until constant weight was obtained to obtain the powder of Phellinus linteus polysaccharide extract.

[0073] Comparative Example 5 This comparative example provides an essential oil for releasing fascia with Bian knife therapy, which differs from Example 1 in that: The Paris polyphylla saponin extract was prepared according to the following method: (1) Weigh 1000 grams of dried Paris rhizome, remove impurities, cut into pieces and crush with a high-speed pulverizer, and pass through an 80-mesh sieve to obtain fine Paris rhizome powder.

[0074] (2) Mix 1000g of fine powder of Paris polyphylla with 12000ml of 60% ethanol and heat to reflux. Maintain reflux extraction for 2 hours. After extraction, filter with multiple layers of gauze while hot and collect the filtrate. Extract the residue with 6000ml of 60% ethanol for 1 hour and filter again. Combine the two filtrates.

[0075] (3) The combined filtrate was concentrated to 1 / 5 of its original volume under reduced pressure at 50°C using a rotary evaporator. After cooling to room temperature, an equal volume of anhydrous ethanol was slowly added, and the mixture was stirred evenly. The mixture was allowed to stand at 4°C for 12 hours. After standing, the precipitate was separated using a refrigerated centrifuge, and the supernatant was collected.

[0076] (4) The supernatant was concentrated under reduced pressure until it became viscous, then transferred to a freeze-drying bottle and freeze-dried at -50°C for 48 hours to obtain Paris polyphylla saponin extract powder.

[0077] Comparative Example 6 This comparative example provides an essential oil for releasing fascia with Bian knife, which differs from Example 1 in that the raw materials for preparing the essential oil, by weight, include: 50 parts sunflower seed oil, 10 parts turmeric root oil, 6 parts ginger root oil, 10 parts peony seed oil, 3 parts tea tree essential oil, 1 part blue chamomile essential oil, 3 parts wintergreen leaf oil, 0.5 parts borneol, and 1 part tocopherol.

[0078] Performance testing 1. Lubricity test, penetration test, and nodule softening effect test Forty volunteers, aged 20-55, were selected. During physical examinations or upon self-reporting palpable myofascial nodules in the shoulders, back, forearms, or other areas, the nodules were confirmed by palpation from two rehabilitation therapists. The nodules were 1-3 cm in diameter, and there was no local skin infection or ulceration. In addition, all volunteers had no serious chronic diseases, no history of skin diseases, no recent use of anti-inflammatory or muscle relaxant drugs, and no broken skin or obvious allergic reactions before the trial.

[0079] In Examples 1-2 and Comparative Examples 1-6, the essential oils were randomly applied sequentially to the inner forearm and nodule areas of the subjects. For the lubricity test, volunteers gently pushed the skin 10 times with their fingers 2 minutes after the essential oil was absorbed, and rated the smoothness, slipperiness, and duration of lubrication on the skin surface, using a score of 1-5 (5 being extremely smooth, 1 being no lubrication). For the penetration test, volunteers evaluated the refreshing feeling, residue, and internal moisturization of the skin surface 1 hour after application, also using a score of 1-5 (5 being extremely refreshing and completely absorbed, 1 being noticeably oily). For the nodule softening effect, after evenly applying the essential oil to the nodule area, a professional therapist massaged it for 5 minutes using a standardized technique. Volunteers rated the softening effect, pain relief, and comfort of the nodules 30 minutes later, with 1 being no change and 5 being very noticeable softening. All scores were the average of 40 participants, and the results were used to compare the performance of different formulations.

[0080] In this test of lubrication, penetration, and nodule softening effect, immediate subjective ratings were used after a single application of the essential oil to evaluate the intuitive improvement felt on the skin and fascia during short-term use. The test results for each group (each example and comparative example) are the average of the immediate experiences of all 40 volunteers, which can reflect the differences in initial experience during actual use.

[0081] 2. Anti-inflammatory test Ninety SPF-grade ICR mice (weighing 20-24g) were randomly divided into four groups: the example group, the comparative groups, and the blank control group, with 10 mice in each group. Inflammation was induced in the right dorsal side of the ear of each mouse in the blank control group and the experimental group by applying a 1% xylene solution. Fifteen minutes later, the mice in the experimental group received the corresponding essential oil, while the blank control group received no treatment. Two hours later, ear discs were taken using a 6mm punch and weighed to calculate the amount of ear swelling. The inflammation inhibition rate of the essential oils in each group was compared to that in the blank control group to evaluate the anti-inflammatory effect.

[0082] Swelling amount = weight of the experimental earpiece (right ear) - weight of the contralateral earpiece (left ear).

[0083] Inflammation suppression rate = [(swelling amount in blank control group - swelling amount in experimental group) / swelling amount in blank control group] × 100%.

[0084] The test results are shown in Table 1.

[0085] Table 1 Performance Test Results Lubricity (points) Permeability (points) Nodule softening effect (points) Anti-inflammatory properties (%) Example 1 4.2 4.1 4.3 67.2 Example 2 4.0 4.0 4.1 63.8 Comparative Example 1 3.5 3.2 3.0 51.4 Comparative Example 2 3.3 3.5 2.8 42.6 Comparative Example 3 3.8 3.0 3.5 48.9 Comparative Example 4 3.6 3.3 3.2 55.1 Comparative Example 5 3.4 3.1 3.3 46.7 Comparative Example 6 4.1 3.9 2.9 38.5 The above results indicate that the essential oils of Examples 1 and 2 exhibit excellent performance in terms of lubricity, permeability, nodule softening effect, and anti-inflammatory properties. This is attributed to their balanced base oil formulation and the use of efficient extraction technology, ensuring high content, high purity, and good absorption of active ingredients, which synergistically promote myofascial release and anti-inflammation. In Comparative Example 1, the base oil ratio was unbalanced, leading to a significant decrease in lubricity and permeability. Simultaneously, the excessively high proportion of ginger root oil may have caused some irritation, interfering with the action of active ingredients and significantly reducing the nodule softening effect and anti-inflammatory properties. In Comparative Example 2, the active ingredient ratio was severely imbalanced; excessive Paris polysaccharides may have increased local irritation, while insufficient Phellinus linteus polysaccharides weakened the ability to promote microcirculation and synergistic effects, resulting in the worst nodule softening effect and anti-inflammatory performance. Comparative Example 3 used traditional distillation instead of the efficient enzymatic hydrolysis + supercritical extraction process to prepare turmeric root oil. The high temperature during distillation caused a significant loss and degradation of heat-sensitive active ingredients (such as curcumin), severely weakening the permeability and anti-inflammatory effects of the essential oil. Comparative Example 4 used a simple hot water reflux extraction method to replace the complex enzymatic hydrolysis process to prepare Phellinus linteus polysaccharides. The polysaccharides extracted by this method had a large molecular weight, poor solubility, and contained many impurities, resulting in poor permeability and ultimately affecting the nodule softening effect and anti-inflammatory properties. Comparative Example 5 used conventional ethanol reflux extraction combined with a simple precipitation method to replace the ion liquid-assisted + resin purification process to prepare Paris polyphylla saponins. The saponins extracted by this method had low purity and contained many lipid-soluble impurities, which not only hindered permeation and absorption but may also cause mild irritation, leading to a decrease in permeability, nodule softening effect, and anti-inflammatory properties. Comparative Example 6 replaced the key active extract with common essential oils. Although the lubricating properties of the base oil were maintained, these common essential oils lacked specific activity against fascial nodules and deep inflammation, resulting in a significant reduction in the nodule softening effect and anti-inflammatory properties, demonstrating the irreplaceable nature of specific plant extracts.

[0086] The above description represents the preferred embodiments of the present invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. An essential oil for releasing fascia with Bian stone therapy, wherein the raw materials for its preparation, by weight, include: 30-60 parts sunflower seed oil, 5-15 parts turmeric root oil, 3-10 parts ginger root oil, 5-15 parts peony seed oil, 1-5 parts Phellinus linteus polysaccharide extract, 0.2-1.5 parts Paris polyphylla saponin extract, 1-5 parts holly leaf oil, 0.1-1 part borneol, and 0.5-2 parts tocopherol.

2. The essential oil for releasing fascia with Bian stone knife according to claim 1, characterized in that: The turmeric root oil is prepared according to the following method: (1) After drying the turmeric root, pulverize it and sieve it to obtain turmeric root powder. Add water to adjust the water weight ratio to 80%-90% to obtain the enzymatic hydrolysis substrate. Pass nitrogen gas, add cellulase, pectinase and β-glucosidase for enzymatic hydrolysis, inactivate the enzyme, filter and dry to ≤10% moisture to obtain the enzymatic hydrolysis product. (2) The enzymatic hydrolysis products were subjected to supercritical carbon dioxide fractional extraction to obtain the first and second products. (3) Molecular distillation was performed on the second product to obtain a purified product; (4) Combine the first product and the purified product in a mass ratio of (7-9):(1-3) to obtain the final product.

3. The essential oil for releasing fascia with Bian stone knife according to claim 2, characterized in that: The amounts of cellulase, pectinase, and β-glucosidase added are 150-250 U / g of the enzymatic hydrolysate, 40-60 U / g of the enzymatic hydrolysate, and 20-40 U / g of the enzymatic hydrolysate, respectively. The specific conditions for enzymatic hydrolysis are: temperature of 45-50℃, pH of 4.8-5.2, rotation speed of 150-200 rpm, and hydrolysis time of 3-4 hours.

4. The essential oil for releasing fascia with Bian stone knife according to claim 2, characterized in that: In step (2), the supercritical carbon dioxide fractional extraction includes a first stage of low-pressure extraction and a second stage of high-pressure enrichment. The conditions for the first stage of low-pressure extraction are: pressure of 25-28 MPa, temperature of 42-50℃, CO2 flow rate of 2-3 L / min, and extraction time of 1-1.5 h. The first stage product is collected by a primary separator. The conditions for the second-stage high-pressure enrichment are: pressure 38-40 MPa, temperature 55-65℃, CO2 flow rate 1.5-2 L / min, entrainer flow rate 5%-8% of CO2 flow rate, extraction time 2-2.5 h, and the second-stage product is collected through a secondary separator; the entrainer is a 95% ethanol aqueous solution by mass.

5. The essential oil for releasing fascia with Bian stone knife according to claim 1, characterized in that: The Phellinus linteus polysaccharide extract was prepared according to the following method: (1) The dried Phellinus linteus fruiting body is crushed and sieved to obtain Phellinus linteus powder. The Phellinus linteus powder is mixed with anhydrous ethanol and degreased to obtain degreased Phellinus linteus powder. (2) Mix defatted Phellinus linteus powder with water at a ratio of 1:15-1:20, soak at 40-45℃ for 30-60 minutes to obtain Phellinus linteus aqueous suspension, add cellulase, pectinase and chitosanase to Phellinus linteus aqueous suspension in sequence, inactivate enzymes after enzymatic hydrolysis, filter and collect filtrate to obtain polysaccharide extract. (3) Concentrate the polysaccharide extract to 20-30% of its original volume, add anhydrous ethanol, let stand, filter, collect the precipitate, wash the precipitate, and obtain crude polysaccharide precipitate; (4) Add water to the crude polysaccharide precipitate to obtain a crude polysaccharide solution. Then add trichloroacetic acid to the crude polysaccharide solution, let it stand in an ice bath, add n-butanol, centrifuge to remove solid impurities, and obtain a deproteinized polysaccharide solution. (5) The polysaccharide solution after deproteinization was dialyzed, and the dialyzed polysaccharide solution was freeze-dried to obtain the final Phellinus linteus polysaccharide extract.

6. The essential oil for releasing fascia with Bian stone knife according to claim 5, characterized in that: The amounts of cellulase, pectinase, and chitosanase added are 200-300 U / g of Phellinus linteus aqueous suspension, 30-50 U / g of Phellinus linteus aqueous suspension, and 50-80 U / g of Phellinus linteus aqueous suspension, respectively; the conditions for enzymatic hydrolysis are: pH value of 4.8-5.2, enzymatic hydrolysis temperature of 45-50℃, and enzymatic hydrolysis time of 3-5 hours; the amount of trichloroacetic acid added in step (3) is 4-6% of the mass of the crude polysaccharide solution, and the amount of n-butanol added is 1-3% of the mass of the crude polysaccharide solution.

7. The essential oil for releasing fascia with Bian stone knife according to claim 1, characterized in that: The Paris polyphylla saponin extract was prepared according to the following method: (1) Crush the dried Paris rhizomes and sieve them to obtain Paris powder; (2) Mix Paris polyphylla powder with anhydrous ethanol, then add 1-butyl-3-methylimidazolium tetrafluoroborate, extract the mixture by ultrasonication, filter after extraction, collect the filtrate, and concentrate the filtrate. (3) After diluting the concentrated filtrate, pass it through a D101 macroporous adsorption resin column, wash off impurities with water, and then elute with ethanol to collect the eluent containing saponins. (4) The eluent was concentrated and freeze-dried to obtain Paris polyphylla saponin extract.

8. The essential oil for releasing fascia with Bian stone knife according to claim 7, characterized in that: In step (2), the mass ratio of Paris polyphylla powder to anhydrous ethanol is 1:(10-15), and the mass of 1-butyl-3-methylimidazolium tetrafluoroborate is 1-2% of the mass of Paris polyphylla powder; the ultrasonic extraction temperature in step (2) is 45-55℃, the ultrasonic power is 300-500W, and the extraction time is 40-60 minutes.

9. A method for preparing an essential oil for fascia release using Bian stone therapy, as described in any one of claims 1-8, comprising the following steps: (1) Mix sunflower seed oil and peony seed oil and stir to obtain a base oil mixture; (2) Add turmeric root oil, ginger root oil and holly leaf oil to the above base oil mixture, and continue stirring to obtain the mixed oil solution; (3) Take 10-15 parts of the mixed oil solution, add the Phellinus linteus polysaccharide extract dissolved in deionized water, and shear to obtain Phellinus linteus polysaccharide dispersion; take another 10-15 parts of the mixed oil solution, add the Paris polyphylla saponin extract dissolved in deionized water, and stir to form Paris polyphylla saponin dispersion; add the Phellinus linteus polysaccharide dispersion and Paris polyphylla saponin dispersion to the remaining mixed oil solution, stir, and obtain the active ingredient mixed oil solution; (4) Add borneol and tocopherol to the above-mentioned active ingredient mixture, stir, filter, and obtain the essential oil for fascia release by Bian knife.

10. The use of the essential oil for fascia release by Bian knife as described in any one of claims 1-8 in the preparation of fascia rehabilitation products, muscle fascia release products, chronic soft tissue injury repair products, pain relief products, physical therapy massage products, and products assisting in Bian knife fascia release operations.