Polypeptide synthesis reaction device for biological medicine manufacturing and operation method of polypeptide synthesis reaction device

Abstract

The invention belongs to the field of polypeptide synthesis reaction, and particularly relates to a polypeptide synthesis reaction device for biological medicine manufacturing and an operation method thereof.The polypeptide synthesis reaction device comprises a base, a side plate is fixedly connected to the top of the base, a supporting plate is slidably connected to the inner wall of the side plate, and a hydraulic cylinder is fixedly connected to the position, located below the supporting plate, in the side plate; according to the polypeptide synthesis reaction device for biological medicine manufacturing and the operation method of the polypeptide synthesis reaction device, the baffle is inserted into the sliding groove, the bottom of the reaction tank is blocked, the baffle in the sliding groove is pulled out, so that the bottom of the reaction tank is not blocked, polypeptide synthesis reactants in the reaction tank directly fall down, the polypeptide synthesis reactants are convenient to take, and the operation efficiency is improved. The reaction tank is convenient to clean, the reaction tank after discharging is controlled to move downwards, the reaction tank is vibrated downwards multiple times through cooperation of a plurality of protruding plates and clamping blocks, polypeptide synthesis reactants adhering to the interior of the reaction tank can be conveniently shaken off, and waste caused by the fact that the polypeptide synthesis reactants adhere to the interior of the reaction tank is avoided.

Description

Technical Field

[0001] This invention belongs to the field of polypeptide synthesis reaction, specifically a polypeptide synthesis reaction apparatus and its operation method for biopharmaceutical manufacturing. Background Technology

[0002] Polypeptides are compounds formed by amino acids linked by amide bonds, and their length is usually less than 50 amino acids. Polypeptides play an important role in the life activities of organisms. Polypeptide drugs have the advantages of low dosage, high efficacy, strong specificity, and few side effects, and are widely used in the treatment of diseases such as diabetes, tumors, cardiovascular diseases, reproductive diseases, and immune diseases. Polypeptide synthesis is formed by the dehydration condensation of different amino acids. Based on this reaction principle, the absence of water is particularly important in the process of polypeptide synthesis. Polypeptides are generally required in small quantities, usually measured in milligrams, but the types are quite extensive. This often requires workers to synthesize multiple polypeptides at once. Polypeptide solid synthesis reactions are generally carried out in a closed reaction vessel.

[0003] However, some peptide synthesis reactants are quite viscous and easily adhere to the inside of the reaction vessel, resulting in a large amount of peptide synthesis reactants remaining on the vessel after feeding, causing waste of peptide synthesis reactants. Moreover, most existing reaction vessels are tank-like structures with relatively narrow feeding ports, making it inconvenient to clean the reaction vessel after removing the peptide synthesis reactants. Residual peptide fragments and raw materials may react unexpectedly with substances in the next batch of reaction system, generating heteropeptides or other impurities, leading to a decrease in the purity of the target product, or even the inability to obtain a qualified product. In addition, when peptide synthesis reactants react in the reaction vessel, inert gases such as nitrogen are usually added. Existing technology can only dilute the gas in the empty space inside the reaction vessel after introducing nitrogen. However, viscous peptide synthesis reactants are prone to leaving cavities inside during stirring, resulting in gas residue. Nitrogen directly introduced into the reaction vessel cannot dilute the gas inside the peptide synthesis reactants, causing the oxygen in the gas to oxidize the peptide synthesis reactants.

[0004] Therefore, the present invention provides a polypeptide synthesis reaction apparatus for biopharmaceutical manufacturing and its operation method. Summary of the Invention

[0005] To address the shortcomings of existing technologies and solve the problem that some peptide synthesis reactants are viscous and easily adhere to the reaction vessel, resulting in a large amount of peptide synthesis reactant residue after feeding and causing waste, this invention proposes a peptide synthesis reaction apparatus and its operating method for biopharmaceutical manufacturing. This is because such reactants are often viscous and tend to adhere to the reaction vessel, leading to waste. Furthermore, most existing reaction vessels are tank-like structures with narrow feed openings, making cleaning difficult after removing the reactants. Residual peptide fragments and raw materials can react unexpectedly with substances in the next batch of reaction system, generating impurities such as heteropeptides, resulting in decreased purity of the target product or even failure to obtain a qualified product. Additionally, when peptide synthesis reacts in the reaction vessel, inert gases such as nitrogen are usually added. Existing technologies only dilute the gas in empty spaces within the reaction vessel after introducing nitrogen. However, viscous peptide synthesis reactants easily leave cavities during stirring, resulting in gas residue. Directly introducing nitrogen into the reaction vessel cannot dilute the gas inside the peptide synthesis reactants, leading to oxidation of the reactants by oxygen in the gas.

[0006] The technical solution adopted by the present invention to solve its technical problem is as follows: A polypeptide synthesis reaction device for biopharmaceutical manufacturing, as described in the present invention, includes a base, a side plate fixedly connected to the top of the base, a support plate slidably connected to the inner wall of the side plate, a hydraulic cylinder fixedly connected inside the side plate and below the support plate, the output end of the hydraulic cylinder fixedly connected to the support plate, two sets of sliding shafts slidably connected to the inner wall of the support plate, a reaction vessel fixedly connected to the bottom of the two sets of sliding shafts, a limit block fixedly connected to the top of each of the two sliding shafts, a feed inlet provided at the top of the reaction vessel, a sliding groove provided inside the reaction vessel, a baffle slidably connected to the inner wall of the sliding groove, a vibration component provided on the outer wall of the side plate, a gas supply pipe fixedly connected to one side of the reaction vessel, and a pressure relief valve provided at the top of the reaction vessel.

[0007] Preferably, the vibration assembly includes a bracket, which is fixedly installed on the outer wall of the side plate and located below the reaction vessel. A mounting frame is fixedly connected to one side of the bracket. Two sets of locking blocks are symmetrically slidably connected to the inner wall of the mounting frame. The top and bottom of the two locking blocks are both set as inclined surfaces. A second spring is fixedly connected to one side of the locking block. The second spring is fixedly connected to the mounting frame. Two sets of protruding plates are fixedly connected to both sides of the reaction vessel. Each set of protruding plates includes several plates that are equidistantly arranged. A first spring is sleeved on the outer wall of the sliding shaft. The top of the first spring is fixedly connected to the support plate, and the bottom of the first spring is fixedly connected to the reaction vessel. The elastic force of the second spring is greater than that of the first spring.

[0008] Preferably, the reaction vessel has two rubber pads symmetrically slidably connected inside, the bottom of both rubber pads extending into the interior of the groove, the bottom of the rubber pads being set as an inclined surface, and a third spring being fixedly connected to the top of the rubber pads, the third spring being fixedly connected to the reaction vessel.

[0009] Preferably, a guide seat is fixedly connected to the outer wall of the side plate and below the mounting bracket. The top of the guide seat is set as an inclined surface. Two partitions are symmetrically fixedly connected to the top of the guide seat. Two guide plates are symmetrically fixedly connected to the inclined surface of the top of the guide seat. Both guide plates are installed at an angle. A groove is provided at the bottom of the guide seat.

[0010] Preferably, the base has a water storage chamber inside, and a water inlet groove is formed at the top of the base, which communicates with the water storage chamber. A first slider is slidably connected inside the guide seat, and a water pump is fixedly connected to the bottom of the first slider. A water pump is provided on the outer wall of the water pump, and the bottom of the water pump penetrates the water inlet groove and extends into the water storage chamber. An annular pipe is fixedly connected to the outer wall of the water pump, and several nozzles are fixedly connected at equal intervals on the outer wall of the annular pipe above the guide seat. An adjustment component is provided inside the guide seat.

[0011] Preferably, the adjustment assembly includes a first motor, which is fixedly installed inside the guide seat. The output end of the first motor is fixedly connected to a first lead screw, which is rotatably connected to the guide seat. The first slider is disposed on the outer wall of the first lead screw, and the first lead screw and the first slider are connected by a lead screw and nut pair.

[0012] Preferably, the inner wall of the reaction vessel is symmetrically fixedly connected with two protective plates, both of which are installed at an angle. The bottom of each protective plate is fixedly connected with several protrusions at equal intervals, and both sides of each protrusion are set as inclined surfaces.

[0013] Preferably, a second motor is fixedly connected to the outer wall of the reaction vessel. The output end of the second motor extends into the interior of the reaction vessel and is fixedly connected to a second lead screw. The second lead screw is located above the two protective plates and is rotatably connected to the reaction vessel. A second slider is connected to the outer wall of the second lead screw via a screw-nut pair. The second slider is slidably connected to the reaction vessel. A sliding plate is slidably connected to the inner wall of the second slider. A third slider is fixedly connected to the bottom of the sliding plate. A support shaft is fixedly connected to the bottom of the third slider. A plurality of lower pressure plates are fixedly connected at equal intervals to the outer wall of the support shaft. The top of the lower pressure plates is configured as an annular inclined surface.

[0014] Preferably, two guide shafts are symmetrically fixedly connected to both sides of the third slider, and two guide grooves are symmetrically opened on the inner wall of the reaction vessel. Both guide grooves are wavy, and one end of the guide shaft extends into the interior of the guide groove.

[0015] A method for operating a polypeptide synthesis reactor for biopharmaceutical manufacturing, applicable to the aforementioned polypeptide synthesis reactor for biopharmaceutical manufacturing, comprising the following steps:

[0016] S1: Insert the baffle into the chute to seal the bottom of the reaction vessel, load the reaction raw materials into the reaction vessel, and introduce nitrogen gas into the reaction vessel through the gas pipeline. Excess gas in the reaction vessel is discharged through the pressure relief valve.

[0017] S2: Start the second motor, drive the second lead screw to rotate, so that the lower pressure plate moves laterally. The guide groove guides the guide shaft, so that the lower pressure plate moves back and forth up and down while moving laterally.

[0018] S3: Pull out the baffle to feed the peptide synthesis reactants into the reaction vessel. After controlling the reaction vessel to move downwards, spray clean water through the nozzle to rinse the inside of the reaction vessel.

[0019] The beneficial effects of this invention are as follows:

[0020] 1. The present invention discloses a polypeptide synthesis reaction apparatus and its operating method for biopharmaceutical manufacturing. By inserting a baffle into a chute to seal the bottom of the reaction vessel, and then removing the baffle from the chute, the bottom of the reaction vessel is unobstructed, allowing the polypeptide synthesis reactants inside the reaction vessel to fall directly. This facilitates the removal of the polypeptide synthesis reactants and the cleaning of the reaction vessel. The reaction vessel is controlled to move downward after being fed. Through the cooperation of several convex plates and locking blocks, the reaction vessel is vibrated downward multiple times, which helps to shake off the polypeptide synthesis reactants adhering to the reaction vessel and avoids waste caused by the polypeptide synthesis reactants sticking to the reaction vessel.

[0021] 2. The present invention discloses a polypeptide synthesis reaction apparatus and its operation method for biopharmaceutical manufacturing. The polypeptide synthesis reactant is guided by the inclined surface at the top of the guide seat, so that the polypeptide synthesis reactant flows towards the end of the inclined surface of the guide seat. When the polypeptide synthesis reactant passes through two guide plates, it flows downward between the two guide plates, thereby making the discharge of the polypeptide synthesis reactant more concentrated. In addition, the groove at the bottom of the guide seat facilitates the collection of the polypeptide synthesis reactant.

[0022] 3. The present invention discloses a polypeptide synthesis reaction apparatus and its operation method for biopharmaceutical manufacturing. Water is sprayed upwards from a nozzle, facilitating internal cleaning of the reaction vessel. The water falling from the nozzle onto a guide seat guides the water, making collection and treatment easier. As the water slides down the inclined surface of the guide seat, it facilitates cleaning of the top of the guide seat, preventing residue of polypeptide synthesis reactants. The water sprayed from the nozzle impacts the inclined surface of the protrusion, and the rebound from the bottom of the protrusion causes the rinsing water to splash outwards, thereby increasing the rinsing range and facilitating cleaning of any areas missed during direct rinsing.

[0023] 4. The polypeptide synthesis reaction apparatus and its operation method for biopharmaceutical manufacturing described in this invention utilize a second motor to control the lateral movement of a second slider, which in turn drives the lower pressure plate to move laterally. This facilitates the stirring of the reaction materials added to the reaction vessel, ensuring uniform mixing of the reaction materials. Simultaneously with the lateral movement of the second slider, the lower pressure plate moves up and down repeatedly through the cooperation of a guide shaft and a guide groove. When the lower pressure plate moves upward, the reaction materials slide down the inclined surface at the top of the lower pressure plate. When the lower pressure plate moves downward, it squeezes the reaction materials below, expelling the gaps within the reaction materials and allowing the gas in the gaps to fill. Combined with the introduction of nitrogen, this prevents residual gas inside the reaction materials from participating in the reaction, which could lead to abnormal product structure. Attached Figure Description

[0024] The invention will now be further described with reference to the accompanying drawings.

[0025] Figure 1 This is a perspective view of the base and guide seat of the present invention in use;

[0026] Figure 2 This is a perspective view of the base of the present invention used in conjunction with the reaction vessel;

[0027] Figure 3 This is a cross-sectional view of the guide seat of the present invention used in conjunction with the reaction vessel;

[0028] Figure 4 This is a perspective view of the water pumping pipe and the annular pipe used in conjunction with the present invention;

[0029] Figure 5 This is a perspective view of the support plate of the present invention used in conjunction with the reaction vessel;

[0030] Figure 6 This is an exploded view of the first slider and the second slider of the present invention used together;

[0031] Figure 7 This is an exploded view of the nozzle and protrusion used in conjunction with the present invention;

[0032] Figure 8This is an exploded view of the guide shaft and guide groove of the present invention in use;

[0033] Figure 9 This is an exploded view of the rubber pad and baffle used in conjunction with the present invention;

[0034] Figure 10 This is an exploded view of the reaction vessel and guide seat of the present invention in use;

[0035] In the diagram: 1. Base; 2. Side plate; 3. Hydraulic cylinder; 4. Support plate; 5. Sliding shaft; 6. First spring; 7. Limiting block; 8. Reaction tank; 9. Feed inlet; 10. Gas pipeline; 11. Pressure relief valve; 12. Slide groove; 13. Baffle; 14. Protruding plate; 15. Bracket; 16. Mounting bracket; 17. Locking block; 18. Second spring; 19. Third spring; 20. Rubber pad; 21. Guide seat; 22. Partition plate; 23. Guide... 24. Water storage chamber; 25. Water inlet tank; 26. First motor; 27. First lead screw; 28. First slider; 29. ​​Pumping pipe; 30. Annular pipe; 31. Nozzle; 32. Water pump; 33. Protective plate; 34. Protrusion; 35. Second motor; 36. Second lead screw; 37. Second slider; 38. Slide plate; 39. Third slider; 40. Support shaft; 41. Lower pressure plate; 42. Guide shaft; 43. Guide groove. Detailed Implementation

[0036] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below in conjunction with specific embodiments.

[0037] like Figures 1 to 10As shown, the present invention provides a technical solution: a polypeptide synthesis reaction apparatus for biopharmaceutical manufacturing, comprising a base 1, a side plate 2 fixedly connected to the top of the base 1, a support plate 4 slidably connected to the inner wall of the side plate 2, a hydraulic cylinder 3 fixedly connected inside the side plate 2 and below the support plate 4, the output end of the hydraulic cylinder 3 being fixedly connected to the support plate 4, two sets of sliding shafts 5 slidably connected to the inner wall of the support plate 4, a reaction vessel 8 fixedly connected to the bottom of the two sets of sliding shafts 5, a limit block 7 fixedly connected to the top of each of the two sliding shafts 5, a feed inlet 9 provided at the top of the reaction vessel 8, a sliding groove 12 provided inside the reaction vessel 8, a baffle 13 slidably connected to the inner wall of the sliding groove 12, a vibration assembly provided on the outer wall of the side plate 2, and a gas supply pipe 10 fixedly connected to one side of the reaction vessel 8. A pressure relief valve 11 is provided at the top; the vibration assembly includes a bracket 15, which is fixedly installed on the outer wall of the side plate 2 and located below the reaction vessel 8. A mounting bracket 16 is fixedly connected to one side of the bracket 15. Two sets of locking blocks 17 are symmetrically slidably connected to the inner wall of the mounting bracket 16. The top and bottom of the two locking blocks 17 are both set as inclined surfaces. A second spring 18 is fixedly connected to one side of the locking block 17. The second spring 18 is fixedly connected to the mounting bracket 16. Two sets of protruding plates 14 are fixedly connected to both sides of the reaction vessel 8. Each set of protruding plates 14 includes several and is equidistant. A first spring 6 is sleeved on the outer wall of the sliding shaft 5. The top of the first spring 6 is fixedly connected to the support plate 4, and the bottom of the first spring 6 is fixedly connected to the reaction vessel 8. The elastic force of the second spring 18 is greater than that of the first spring 6.

[0038] Through the above technical solution, the baffle 13 is inserted into the chute 12 to seal the bottom of the reaction tank 8. Reaction materials are loaded into the reaction tank 8 through the feed inlet 9, and inert gases such as nitrogen are introduced into the reaction tank 8 through the gas supply pipe 10. Excess gas in the reaction tank 8 is discharged through the pressure relief valve 11, thereby isolating the reaction materials from oxygen. After the polypeptide synthesis reaction is completed, the baffle 13 in the chute 12 is removed, removing the obstruction at the bottom of the reaction tank 8. The polypeptide synthesis reaction material in the reaction tank 8 then falls directly down, facilitating the removal of the polypeptide synthesis reaction material. The hydraulic cylinder 3 controls the support plate 4 to move downwards, causing the reaction tank 8 to move downwards, moving the convex plate 14 into the mounting bracket 16. At this time, under the action of the second spring 18... The inclined surface at the top of the locking block 17 presses against the protruding plate 14. Under the limitation of the locking block 17, as the support plate 4 moves downward, the position of the reaction vessel 8 remains unchanged, pressing the first spring 6. When the first spring 6 is pressed to its limit, the protruding plate 14 presses against the inclined surface at the top of the locking block 17. Under the pressure of the protruding plate 14, the locking block 17 moves into the mounting frame 16, pressing the second spring 18. Without the limiting axis of the locking block 17, under the action of the first spring 6, the reaction vessel 8 moves downward quickly and vibrates. Thus, through the several protruding plates 14, as the support plate 4 moves downward, the reaction vessel 8 vibrates downward multiple times, which facilitates the shaking off of the polypeptide synthesis reactants adhering to the reaction vessel 8, avoiding the polypeptide synthesis reactants sticking to the reaction vessel 8 and causing waste.

[0039] Specifically, two rubber pads 20 are symmetrically slidably connected inside the reaction vessel 8. The bottom of both rubber pads 20 extends into the interior of the groove 12. The bottom of the rubber pads 20 is set as an inclined surface. A third spring 19 is fixedly connected to the top of the rubber pads 20. The third spring 19 is fixedly connected to the reaction vessel 8.

[0040] With the above technical solution, when the baffle 13 is inserted into the slide groove 12, the baffle 13 presses against the inclined surface at the bottom of the rubber pad 20. Under the push of the baffle 13, the rubber pad 20 moves upward and presses the third spring 19. After the baffle 13 is fully inserted into the slide groove 12, under the action of the two third springs 19, the two rubber pads 20 pass through and press against the top of the baffle 13, thereby facilitating the sealing of the connection between the baffle 13 and the slide groove 12 and preventing liquid leakage.

[0041] Specifically, a guide seat 21 is fixedly connected to the outer wall of the side plate 2 and below the mounting bracket 16. The top of the guide seat 21 is set as an inclined surface. Two partitions 22 are symmetrically fixedly connected to the top of the guide seat 21. Two guide plates 23 are symmetrically fixedly connected to the inclined surface at the top of the guide seat 21. Both guide plates 23 are installed at an angle. A groove is provided at the bottom of the guide seat 21.

[0042] With the above technical solution, when the baffle 13 is pulled out to feed the polypeptide synthesis reactants, the polypeptide synthesis reactants fall on the inclined surface of the guide seat 21 and slide down along the inclined surface of the guide seat 21. When passing through the two guide plates 23, they flow downward between the two guide plates 23, thereby making the feeding of polypeptide synthesis reactants more concentrated. In addition, the groove at the bottom of the guide seat 21 facilitates the collection of polypeptide synthesis reactants.

[0043] Specifically, the base 1 has a water storage chamber 24 inside, and a water inlet groove 25 on the top of the base 1, which communicates with the water storage chamber 24. A first slider 28 is slidably connected inside the guide seat 21. A water pumping pipe 29 is fixedly connected to the bottom of the first slider 28. A water pump 32 is installed on the outer wall of the water pumping pipe 29. The bottom of the water pumping pipe 29 passes through the water inlet groove 25 and extends into the water storage chamber 24. An annular pipe 30 is fixedly connected to the outer wall of the water pumping pipe 29. Several nozzles 31 are fixedly connected at equal intervals on the outer wall of the annular pipe 30 and above the guide seat 21. An adjustment assembly is installed inside the guide seat 21. The adjustment assembly includes a first motor 26, which is fixedly installed inside the guide seat 21. A first lead screw 27 is fixedly connected to the output end of the first motor 26. The first lead screw 27 is rotatably connected to the guide seat 21. The first slider 28 is located on the outer wall of the first lead screw 27. The first lead screw 27 and the first slider 28 are connected by a lead screw and nut pair.

[0044] Through the above technical solution, clean water is filled into the water storage chamber 24 through the water inlet tank 25. After the polypeptide synthesis reactants in the reaction tank 8 are fed, the water pump 32 is started to pump the clean water in the water storage chamber 24 along the water pumping pipe 29 and the annular pipe 30 to the nozzle 31. The clean water is sprayed upward through the nozzle 31, which facilitates the internal cleaning of the reaction tank 8. The clean water sprayed into the reaction tank 8 can fall onto the guide seat 21, which can also guide the falling clean water, making it easy to collect and treat the sprayed clean water. When water slides down the inclined surface of the guide seat 21, it facilitates cleaning of the top of the guide seat 21 and avoids residue of peptide synthesis reactants. The first motor 26 is started, which drives the first lead screw 27 to rotate, causing the first slider 28 to move and drive the nozzle 31 to move laterally. This makes it easy to adjust the position of the nozzle 31 to rinse different positions in the reaction tank 8. After rinsing, the position of the nozzle 31 is adjusted by the first motor 26 so that the nozzle 31 moves to the leftmost position to prevent peptide synthesis reactants from falling on the nozzle 31 when the reaction tank 8 is being fed.

[0045] Specifically, the inner wall of the reaction vessel 8 is symmetrically fixedly connected with two protective plates 33. Both protective plates 33 are installed at an angle. Several protrusions 34 are fixedly connected at equal intervals at the bottom of the protective plates 33. Both sides of the protrusions 34 are set as inclined surfaces.

[0046] Through the above technical solution, the clean water sprayed from the nozzle 31 impacts the inclined surface of the protrusion 34. Under the rebound of the inclined surface at the bottom of the protrusion 34, the clean water splashes in all directions, thereby increasing the rinsing range of the clean water and making it easier to clean the areas missed by direct rinsing.

[0047] Specifically, a second motor 35 is fixedly connected to the outer wall of the reaction vessel 8. The output end of the second motor 35 extends into the interior of the reaction vessel 8 and is fixedly connected to a second lead screw 36. The second lead screw 36 is located above the two protective plates 33 and is rotatably connected to the reaction vessel 8. A second slider 37 is connected to the outer wall of the second lead screw 36 through a screw-nut pair. The second slider 37 is slidably connected to the reaction vessel 8. A slide plate 38 is slidably connected to the inner wall of the second slider 37. A third slider 39 is fixedly connected to the bottom of the slide plate 38. A support shaft 40 is fixedly connected to the bottom of the third slider 39. Several lower pressure plates 41 are fixedly connected at equal intervals to the outer wall of the support shaft 40. The top of the lower pressure plates 41 is set as an annular inclined surface.

[0048] Through the above technical solution, the second motor 35 is started, which drives the second lead screw 36 to rotate, causing the second slider 37 to move laterally, which in turn drives the slide plate 38 to move laterally, causing the third slider 39 to move laterally, which in turn drives the support shaft 40 to move laterally, causing the lower pressure plate 41 to move laterally. The laterally moving lower pressure plate 41 facilitates the stirring of the reaction raw materials put into the reaction tank 8, so that the reaction raw materials are mixed evenly.

[0049] Specifically, two guide shafts 42 are symmetrically fixedly connected to both sides of the third slider 39, and two guide grooves 43 are symmetrically opened on the inner wall of the reaction vessel 8. Both guide grooves 43 are set as wave-shaped, and one end of the guide shaft 42 extends into the interior of the guide groove 43.

[0050] With the above technical solution, when the third slider 39 moves laterally, it drives the two guide shafts 42 to move laterally in the guide grooves 43 respectively. Through the guidance of the guide grooves 43, the two guide shafts 42 move back and forth up and down while moving laterally, which drives the third slider 39 to move back and forth up and down while moving laterally, and causes the lower pressure plate 41 to move back and forth up and down while moving laterally. When the lower pressure plate 41 moves upward, the reaction raw materials slide down the inclined surface at the top of the lower pressure plate 41. When the lower pressure plate 41 moves downward, it squeezes the multiple reaction raw materials below, discharges the gaps in the reaction raw materials, and allows the gas in the gaps to fill in. With the introduction of nitrogen, it avoids the residual gas inside the reaction raw materials from participating in the reaction, which would lead to abnormal product structure.

[0051] A method for operating a polypeptide synthesis reactor for biopharmaceutical manufacturing, applicable to the aforementioned polypeptide synthesis reactor for biopharmaceutical manufacturing, comprising the following steps:

[0052] S1: Insert the baffle 13 into the slide groove 12 to seal the bottom of the reaction tank 8, load the reaction raw materials into the reaction tank 8, and introduce nitrogen gas into the reaction tank 8 through the gas pipeline 10. Excess gas in the reaction tank 8 is discharged through the pressure relief valve 11.

[0053] S2: Start the second motor 35, drive the second lead screw 36 to rotate, so that the lower pressure plate 41 moves laterally, and guides the guide shaft 42 through the guide groove 43, so that the lower pressure plate 41 moves back and forth up and down while moving laterally;

[0054] S3: Pull out the baffle 13 to feed the polypeptide synthesis reactants into the reaction vessel 8. After controlling the reaction vessel 8 to move downward, spray clean water through the nozzle 31 to rinse the inside of the reaction vessel 8.

[0055] In use, the baffle 13 is inserted into the chute 12 to seal the bottom of the reaction vessel 8. When the baffle 13 is inserted into the chute 12, it presses against the inclined surface of the bottom of the rubber pad 20. Under the push of the baffle 13, the rubber pad 20 moves upward and compresses the third spring 19. After the baffle 13 is fully inserted into the chute 12, the two rubber pads 20 are pressed against the top of the baffle 13 by the action of the two third springs 19. This facilitates the sealing of the connection between the baffle 13 and the chute 12, preventing liquid leakage. The reaction raw materials are loaded into the reaction vessel 8 through the feed inlet 9, and inert gases such as nitrogen are introduced into the reaction vessel 8 through the gas supply pipe 10. Excess gas in the reaction vessel 8 is discharged through the pressure relief valve 11, thereby isolating the reaction raw materials from oxygen. In the gas reaction, the second motor 35 is activated, driving the second lead screw 36 to rotate, causing the second slider 37 to move laterally, which in turn causes the slide plate 38 to move laterally, causing the third slider 39 to move laterally, which in turn causes the support shaft 40 to move laterally, and the lower pressure plate 41 to move laterally. The laterally moving lower pressure plate 41 facilitates the stirring of the reaction materials added to the reaction tank 8, ensuring uniform mixing. Simultaneously, as the third slider 39 moves laterally, it drives the two guide shafts 42 to move laterally within the guide grooves 43. Guided by the guide grooves 43, the two guide shafts 42 reciprocate up and down while moving laterally, causing the third slider 39 to reciprocate up and down while moving laterally, and the lower pressure plate 41 to reciprocate up and down while moving laterally. When the lower pressure plate 41 moves towards... As the reaction material moves upward, it slides down the inclined surface at the top of the lower pressure plate 41. When the lower pressure plate 41 moves downward, it squeezes the reaction material below, expelling the gaps in the reaction material and allowing the gas in the gaps to fill. Combined with the introduction of nitrogen, this prevents residual gas inside the reaction material from participating in the reaction and causing abnormal product structure. After the peptide synthesis reaction is completed, the baffle 13 in the chute 12 is pulled out, so that the bottom of the reaction tank 8 is unobstructed. Thus, the peptide synthesis reaction material in the reaction tank 8 falls directly, making it easier to remove the peptide synthesis reaction material. The hydraulic cylinder 3 controls the support plate 4 to move downward, driving the reaction tank 8 downward, so that the convex plate 14 moves into the mounting frame 16. At this time, under the action of the second spring 18, the inclined surface at the top of the clamping block 17 presses against the convex plate. 14. Under the limiting position of the locking block 17, as the support plate 4 moves downward, the position of the reaction vessel 8 remains unchanged, compressing the first spring 6. When the first spring 6 is compressed to its limit, the convex plate 14 presses against the inclined surface at the top of the locking block 17. Under the pressure of the convex plate 14, the locking block 17 moves into the mounting frame 16, compressing the second spring 18. Without the limiting axis of the locking block 17, under the action of the first spring 6, the reaction vessel 8 moves downward rapidly and vibrates. Thus, through the several convex plates 14, as the support plate 4 moves downward, the reaction vessel 8 vibrates downward multiple times, facilitating the shaking off of the polypeptide synthesis reactants adhering to the reaction vessel 8, avoiding waste caused by the polypeptide synthesis reactants sticking inside the reaction vessel 8. When the baffle 13 is pulled out to discharge the polypeptide synthesis reactants...The polypeptide synthesis reactants fall onto the inclined surface of the guide seat 21 and slide down it. As they pass the two guide plates 23, they flow downwards between them, thus concentrating the polypeptide synthesis reactants. The groove at the bottom of the guide seat 21 facilitates collection of the polypeptide synthesis reactants. Water is added to the water storage chamber 24 through the water inlet 25. After the polypeptide synthesis reactants are added to the reaction tank 8, the water pump 32 is activated, drawing the water from the water storage chamber 24 along the pumping pipe 29 and the annular pipe 30 to the nozzle 31. The nozzle 31 sprays the water upwards, facilitating the cleaning of the inside of the reaction tank 8. The water sprayed from the reaction tank 8 also falls onto the guide seat 21, which guides the water, ensuring a smooth flow after spraying. The clean water is easy to collect and treat. When the clean water slides down the inclined surface of the guide seat 21, it facilitates cleaning of the top of the guide seat 21, preventing residue of peptide synthesis reactants. The first motor 26 is activated, driving the first lead screw 27 to rotate, causing the first slider 28 to move, which in turn moves the nozzle 31 laterally. This allows for easy adjustment of the nozzle 31's position to rinse different areas within the reaction tank 8. After rinsing, the first motor 26 adjusts the nozzle 31's position, moving it to the far left to prevent peptide synthesis reactants from falling onto the nozzle 31 during material feeding into the reaction tank 8. The clean water sprayed from the nozzle 31 impacts the inclined surface of the protrusion 34, and the rebound from the bottom of the protrusion 34 causes the rinsing water to splash outwards, thus increasing the rinsing range and facilitating cleaning of areas missed during direct rinsing.

[0056] The terms "front," "back," "left," "right," "top," and "bottom" all refer to the figures in the accompanying drawings. Figure 1 Based on the perspective of the observer, the side of the device facing the observer is defined as the front, the left side of the observer is defined as the left, and so on.

[0057] In the description of this invention, it should be understood that the terms "center", "longitudinal", "lateral", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limiting the scope of protection of this invention.

[0058] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of the present invention is defined by the appended claims and their equivalents.

Claims

1. A polypeptide synthesis reaction apparatus for biopharmaceutical manufacturing, characterized in that, The system includes a base (1), a side plate (2) fixedly connected to the top of the base (1), a support plate (4) slidably connected to the inner wall of the side plate (2), a hydraulic cylinder (3) fixedly connected inside the side plate (2) and below the support plate (4), the output end of the hydraulic cylinder (3) fixedly connected to the support plate (4), two sets of sliding shafts (5) slidably connected to the inner wall of the support plate (4), a reaction tank (8) fixedly connected to the bottom of the two sets of sliding shafts (5), a limit block (7) fixedly connected to the top of each of the two sliding shafts (5), a feed inlet (9) provided at the top of the reaction tank (8), a sliding groove (12) provided inside the reaction tank (8), a baffle (13) slidably connected to the inner wall of the sliding groove (12), a vibration component provided on the outer wall of the side plate (2), a gas supply pipe (10) fixedly connected to one side of the reaction tank (8), and a pressure relief valve (11) provided at the top of the reaction tank (8).

2. The polypeptide synthesis reaction apparatus for biopharmaceutical manufacturing according to claim 1, characterized in that, The vibration assembly includes a bracket (15), which is fixedly installed on the outer wall of the side plate (2) and located below the reaction vessel (8). A mounting bracket (16) is fixedly connected to one side of the bracket (15). Two sets of locking blocks (17) are symmetrically slidably connected to the inner wall of the mounting bracket (16). The top and bottom of the two locking blocks (17) are both set as inclined surfaces. A second spring (18) is fixedly connected to one side of the locking block (17). The second spring (18) is fixedly connected to the mounting bracket (16). Two sets of protruding plates (14) are fixedly connected to both sides of the reaction vessel (8). Each set of protruding plates (14) includes several and is equidistant. A first spring (6) is sleeved on the outer wall of the sliding shaft (5). The top of the first spring (6) is fixedly connected to the support plate (4). The bottom of the first spring (6) is fixedly connected to the reaction vessel (8). The elastic force of the second spring (18) is greater than that of the first spring (6).

3. The polypeptide synthesis reaction apparatus for biopharmaceutical manufacturing according to claim 2, characterized in that, The reaction vessel (8) has two rubber pads (20) symmetrically slidably connected inside. The bottom of both rubber pads (20) extends into the interior of the groove (12). The bottom of the rubber pads (20) is set as an inclined surface. A third spring (19) is fixedly connected to the top of the rubber pads (20). The third spring (19) is fixedly connected to the reaction vessel (8).

4. The polypeptide synthesis reaction apparatus for biopharmaceutical manufacturing according to claim 3, characterized in that, A guide seat (21) is fixedly connected to the outer wall of the side plate (2) and below the mounting bracket (16). The top of the guide seat (21) is set as an inclined surface. Two partitions (22) are symmetrically fixedly connected to the top of the guide seat (21). Two guide plates (23) are symmetrically fixedly connected to the inclined surface at the top of the guide seat (21). Both guide plates (23) are installed at an incline. A groove is provided at the bottom of the guide seat (21).

5. The polypeptide synthesis reaction apparatus for biopharmaceutical manufacturing according to claim 4, characterized in that, The base (1) has a water storage chamber (24) inside, and a water inlet groove (25) is opened on the top of the base (1). The water inlet groove (25) communicates with the water storage chamber (24). A first slider (28) is slidably connected inside the guide seat (21). A water pumping pipe (29) is fixedly connected to the bottom of the first slider (28). A water pump (32) is provided on the outer wall of the water pumping pipe (29). The bottom of the water pumping pipe (29) passes through the water inlet groove (25) and extends into the interior of the water storage chamber (24). An annular pipe (30) is fixedly connected to the outer wall of the water pumping pipe (29). Several nozzles (31) are fixedly connected at equal intervals on the outer wall of the annular pipe (30) and above the guide seat (21). An adjustment component is provided inside the guide seat (21).

6. The polypeptide synthesis reaction apparatus for biopharmaceutical manufacturing according to claim 5, characterized in that, The adjustment assembly includes a first motor (26), which is fixedly installed inside the guide seat (21). The output end of the first motor (26) is fixedly connected to a first lead screw (27). The first lead screw (27) is rotatably connected to the guide seat (21). The first slider (28) is disposed on the outer wall of the first lead screw (27). The first lead screw (27) and the first slider (28) are connected by a lead screw and nut pair.

7. The polypeptide synthesis reaction apparatus for biopharmaceutical manufacturing according to claim 6, characterized in that, The inner wall of the reaction vessel (8) is symmetrically fixedly connected with two protective plates (33). Both protective plates (33) are installed at an angle. Several protrusions (34) are fixedly connected at equal intervals at the bottom of the protective plates (33). Both sides of the protrusions (34) are set as inclined surfaces.

8. The polypeptide synthesis reaction apparatus for biopharmaceutical manufacturing according to claim 7, characterized in that, A second motor (35) is fixedly connected to the outer wall of the reaction vessel (8). The output end of the second motor (35) extends into the interior of the reaction vessel (8) and is fixedly connected to a second lead screw (36). The second lead screw (36) is located above the two protective plates (33). The second lead screw (36) is rotatably connected to the reaction vessel (8). A second slider (37) is connected to the outer wall of the second lead screw (36) through a lead screw nut pair. The second slider (37) is slidably connected to the reaction vessel (8). A sliding plate (38) is slidably connected to the inner wall of the second slider (37). A third slider (39) is fixedly connected to the bottom of the sliding plate (38). A support shaft (40) is fixedly connected to the bottom of the third slider (39). Several lower pressure plates (41) are fixedly connected at equal intervals to the outer wall of the support shaft (40). The top of the lower pressure plate (41) is set as an annular inclined surface.

9. The polypeptide synthesis reaction apparatus for biopharmaceutical manufacturing according to claim 8, characterized in that, The third slider (39) has two guide shafts (42) symmetrically fixedly connected on both sides. The inner wall of the reaction vessel (8) has two guide grooves (43) symmetrically opened. Both guide grooves (43) are set as waveforms. One end of the guide shaft (42) extends into the interior of the guide groove (43).

10. A method for operating a polypeptide synthesis reaction apparatus for biopharmaceutical manufacturing, the method being applicable to the polypeptide synthesis reaction apparatus for biopharmaceutical manufacturing described in claim 9, characterized in that: The steps for this operation are as follows: S1: Insert the baffle (13) into the slide (12) to seal the bottom of the reaction tank (8), load the reaction raw materials into the reaction tank (8), and pass nitrogen gas into the reaction tank (8) through the gas pipeline (10). Excess gas in the reaction tank (8) is discharged through the pressure relief valve (11). S2: Start the second motor (35) to drive the second lead screw (36) to rotate, so that the lower pressure plate (41) moves laterally and guides the guide shaft (42) through the guide groove (43), so that the lower pressure plate (41) moves back and forth up and down while moving laterally; S3: Pull out the baffle (13) to feed the polypeptide synthesis reactants into the reaction vessel (8), control the reaction vessel (8) to move downward, and spray clean water through the nozzle (31) to rinse the inside of the reaction vessel (8).

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