Magnetic microsphere sorting device

Abstract

The invention discloses a magnetic microsphere sorting device which comprises a base and a rectangular shell, the rectangular shell is installed on the base, a micro-flow cavity is defined between the inner side of the rectangular shell and the base, an inlet of the micro-flow cavity is formed in the upper end face of the first end of the rectangular shell, and an outlet of the micro-flow cavity is formed in the second end of the rectangular shell. The distance between the upper wall surface in the micro-flow cavity and the substrate is gradually reduced from an inlet to an outlet of the micro-flow cavity, an electromagnet is arranged on the outer side of the first end of the rectangular shell, the side, close to the inlet, of the micro-flow cavity is parallel to the electromagnet, the outlet of the micro-flow cavity is communicated with the lower end of a cavity channel, and the upper end of the cavity channel extends to the upper end face of the second end of the rectangular shell; the upper end of the cavity channel is communicated with the liquid collecting bottle through a pipeline. According to the method, multiple marker objects in the to-be-detected sample can be detected at the same time, size sorting can be carried out, the condition of a patient is determined through strip image features, and the detection efficiency is high.

Description

Technical Field

[0001] This invention belongs to the field of flow cytometry technology, specifically relating to a magnetic microsphere sorting device. Background Technology

[0002] The existing method for using microfluidic chips for magnetic microsphere sorting involves adding a mixture of magnetic microspheres, fluorescent markers, and antigen-antibody to the microfluidic chip, using the magnetic field in the microfluidic chip to sort the magnetic microspheres into different sorting channels according to their size, and then performing fluorescence detection on the sorted magnetic microspheres.

[0003] However, current microsphere chip sorting uses multiple channels to separate and collect different magnetic microspheres, lacking a solution for simultaneous detection of these three markers. Furthermore, detection requires removing the microspheres from the inspection channel, thus requiring multiple operations of the flow cytometer to detect multiple markers, increasing the operational process and extending the detection time. Summary of the Invention

[0004] This invention proposes a magnetic microsphere sorting device that can simultaneously detect multiple marker objects in a sample and sort them by size, and determine the patient's condition by recognizing the image features of the strips.

[0005] To achieve the above objectives, the technical solution of the present invention is a magnetic microsphere sorting device, comprising a substrate and a rectangular shell. The rectangular shell is mounted on the substrate, and a microfluidic cavity is formed between the inner side of the rectangular shell and the substrate. The upper end face of the first end of the rectangular shell is provided with the inlet of the microfluidic cavity, and the interior of the second end of the rectangular shell is provided with the outlet of the microfluidic cavity. The distance between the upper wall of the microfluidic cavity and the substrate decreases from the inlet to the outlet of the microfluidic cavity. An electromagnet is provided on the outer side of the first end of the rectangular shell, and the side of the microfluidic cavity near the inlet is parallel to the electromagnet. The outlet of the microfluidic cavity is connected to the lower end of a cavity channel, and the upper end of the cavity channel extends to the upper end face of the second end of the rectangular shell. The upper end of the cavity channel is connected to a liquid collection bottle through a pipe.

[0006] The magnetic microsphere sorting device is horizontally installed in the centrifuge device. The magnetic field generated by the electromagnet is perpendicular to the centrifugal force, and the centrifugal force is parallel to the longitudinal direction of the rectangular shell.

[0007] The microfluidic cavity is filled with a carrier fluid. The inlet of the microfluidic cavity is connected to the outlet of the liquid collection bottle through a detachable circulation pipe, which is equipped with a valve that can be remotely opened and closed.

[0008] The substrate and the rectangular shell are made of polydimethylsiloxane, glass, or transparent resin material.

[0009] The upper wall of the microfluidic cavity is constructed as a folded surface, including a first wall near the inlet of the microfluidic cavity and a second wall near the outlet of the microfluidic cavity. The angle between the first wall and the substrate is greater than the angle between the second wall and the substrate. The distance between the second wall and the substrate decreases from 1000 micrometers to 2 micrometers from the inlet to the outlet of the microfluidic cavity.

[0010] The distance from the electromagnet to the microfluidic cavity is 1-25 micrometers.

[0011] Beneficial effects: This invention can simultaneously detect multiple markers in the sample to be tested, and can arrange magnetic microspheres of different sizes in the microfluidic cavity in descending order of particle size from the side of the second wall near the permanent magnet to the outlet of the microfluidic cavity to form multiple strips. Therefore, the patient's condition can be determined simultaneously through the image features of multiple strips, resulting in high detection efficiency. Attached Figure Description

[0012] Figure 1 This is a schematic diagram of the structure of the present invention. Detailed Implementation

[0013] like Figure 1 As shown, Figure 1 As shown, a magnetic microsphere sorting device includes a substrate 1 and a rectangular shell 2. The rectangular shell 2 is disposed on the substrate 1. An electromagnet 6 is disposed opposite to the first end (left end) of the rectangular shell 2 perpendicular to the substrate 1. A microfluidic cavity 3 is formed between the inner side of the rectangular shell 2 and the substrate 1. The side of the microfluidic cavity 3 near the electromagnet 6 is parallel to the electromagnet 6. The distance from the electromagnet 6 to the microfluidic cavity 3 is 1-25 micrometers. The upper end face of the first end of the rectangular shell 2 is provided with the inlet of the microfluidic cavity 3. The second end (right end) of the rectangular shell 2 is provided with the outlet of the microfluidic cavity 3. The distance between the upper wall of the microfluidic cavity 3 and the substrate 1 decreases from the inlet to the outlet of the microfluidic cavity 3. The outlet of the microfluidic cavity 3 is connected to the lower end of a cavity channel 4. The upper end of the cavity channel 4 extends to the upper end face of the second end of the rectangular shell 2. The upper end of the cavity channel 4 is connected to a liquid collection bottle through a pipe.

[0014] The substrate 1 and the rectangular shell 2 are made of polydimethylsiloxane, glass, or transparent resin. The upper wall of the microfluidic cavity 3 is configured as a folded surface, including a first wall near the inlet of the microfluidic cavity 3 and a second wall near the outlet of the microfluidic cavity 3. The angle between the first wall and the substrate 1 is greater than the angle between the second wall and the substrate 1. The distance between the second wall and the substrate 1 decreases from 1000 micrometers to 2 micrometers from the inlet to the outlet of the microfluidic cavity 3.

[0015] Furthermore, the magnetic microsphere sorting device is horizontally installed in the centrifuge device. The magnetic field generated by the electromagnet 6 is perpendicular to the direction of the centrifugal force, which is parallel to the longitudinal direction of the rectangular shell 2. The microfluidic cavity 3 is filled with a carrier fluid. The inlet of the microfluidic cavity 3 is connected to the outlet of the liquid collection bottle through a detachable circulation pipe, which is equipped with a remotely controlled valve.

[0016] The electromagnet 6 is wrapped with a coil. The magnetic field generated by the electromagnet 6 can be adjusted by adjusting the current passing through the coil, so that the centrifugal force can overcome the magnetic force of the magnetic field.

[0017] In use, a variety of magnetic microspheres 7 with different sizes are prepared. In this embodiment, three kinds of magnetic microspheres 7 with different sizes are prepared. The magnetic microspheres 7 of different sizes have the same weight but different volumes. Therefore, the density decreases as the volume increases. The minimum diameter of the magnetic microsphere is greater than the outlet height of the microfluidic cavity. The composite liquid containing magnetic microspheres 7 is added to the inlet of the microfluidic cavity 3 of the magnetic microsphere sorting device; the distance between the electromagnet 6 and the left end of the microfluidic cavity 3 is small enough that the magnetic field generated at the left end of the microfluidic cavity 3 is sufficient to attract the magnetic microspheres 7 to the left end of the microfluidic cavity 3. The magnetic microsphere sorting device is horizontally installed on the turntable of the centrifuge device, so that the magnetic field generated by the electromagnet 6 is perpendicular to the direction of the centrifugal force, and the direction of the centrifugal force is parallel to the longitudinal direction of the rectangular shell 2. The inlet of the microfluidic cavity 3 is connected to the outlet of the liquid collection bottle through a detachable circulation pipe, and the upper end of the cavity channel 4 is connected to the liquid collection bottle through a pipe. The centrifuge device is turned on and the rotation speed is gradually increased. Under the action of centrifugal force, the magnetic microspheres 7 with the largest density and smallest size first overcome the magnetic force of the electromagnet 6. The magnetic microspheres 7 of different sizes flow from the inlet to the outlet of the microfluidic cavity 3 in order of increasing size. After the magnetic microspheres 7 enter the area below the second wall, they are no longer subject to the magnetic force due to the distance from the electromagnet 6. The magnetic microspheres 7 are arranged in order of decreasing particle size from the side of the second wall close to the electromagnet 6 to the outlet of the microfluidic cavity 3, forming multiple strips. Close the valve on the circulation pipe to drain the composite liquid in the microfluidic cavity 3; after draining, stop the centrifugation device in time, and the magnetic microspheres 7 will not flow back to the inlet of the microfluidic cavity 3 under the action of the composite liquid.

[0018] Stop the centrifugation device, remove the magnetic microsphere sorting device, and use the detection system to obtain image features of magnetic microsphere strips of different sizes, thereby obtaining information about the patient's condition.

[0019] While the specific embodiments of this disclosure have been described above in conjunction with the accompanying drawings, this is not intended to limit the scope of protection of this disclosure. Those skilled in the art should understand that various modifications or variations that can be made by those skilled in the art without creative effort based on the technical solutions of this disclosure are still within the scope of protection of this disclosure.

Claims

1. A magnetic microsphere sorting device, characterized in that, The device includes a base (1) and a rectangular shell (2). The rectangular shell (2) is mounted on the base (1). The inner side of the rectangular shell (2) and the base (1) enclose a microfluidic cavity (3). The upper surface of the first end of the rectangular shell (2) is provided with the inlet of the microfluidic cavity (3). The interior of the second end of the rectangular shell (2) is provided with the outlet of the microfluidic cavity (3). The distance between the upper wall of the microfluidic cavity (3) and the base (1) decreases from the inlet to the outlet of the microfluidic cavity (3). An electromagnet (6) is provided on the outer side of the first end of the rectangular shell (2). The side of the microfluidic cavity (3) near the inlet is parallel to the electromagnet (6). The outlet of the microfluidic cavity (3) is connected to the lower end of the cavity channel (4). The upper end of the cavity channel (4) extends to the upper surface of the second end of the rectangular shell (2). The upper end of the cavity channel (4) is connected to the liquid collection bottle through a pipe.

2. The magnetic microsphere sorting device according to claim 1, characterized in that, The magnetic microsphere sorting device is horizontally installed in the centrifuge device. The magnetic field generated by the electromagnet (6) is perpendicular to the centrifugal force direction, and the centrifugal force direction is parallel to the longitudinal direction of the rectangular shell (2).

3. The magnetic microsphere sorting device according to claim 1, characterized in that, The microfluidic cavity (3) is filled with a carrier fluid. The inlet of the microfluidic cavity (3) is connected to the outlet of the liquid collection bottle through a detachable circulation pipe. The circulation pipe is equipped with a valve that can be remotely opened and closed.

4. The magnetic microsphere sorting device according to claim 1, characterized in that, The substrate (1) and the rectangular shell (2) are made of polydimethylsiloxane, glass or transparent resin material.

5. The magnetic microsphere sorting device according to claim 1, characterized in that, The upper wall of the microfluidic cavity (3) is constructed as a folded surface, including a first wall near the inlet of the microfluidic cavity (3) and a second wall near the outlet of the microfluidic cavity (3). The angle between the first wall and the substrate (1) is greater than the angle between the second wall and the substrate (1). The distance between the second wall and the substrate (1) decreases from 1000 micrometers to 2 micrometers from the inlet to the outlet of the microfluidic cavity (3).

6. The magnetic microsphere sorting device according to claim 1, characterized in that, The distance between the electromagnet (6) and the microfluidic cavity (3) is 1-25 micrometers.

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