Gas distribution structure of biological medicine fermentation tank

Abstract

The invention discloses a biological medicine fermentation tank gas distribution structure, and relates to the technical field of biological fermentation tanks, the biological medicine fermentation tank gas distribution structure comprises a support frame seat, a fermentation tank body is mounted in the support frame seat, a control system is mounted at the front end of the support frame seat, and the outer side of the fermentation tank body is sleeved with a protective cylinder; and three groups of built-in multi-layer sensors are arranged in the fermentation tank body. By means of the synergistic effect of mechanical shearing and flow field guiding, the diameter of bubbles can be stably controlled, compared with a traditional structure, the gas-liquid contact area is increased, the oxygen dissolution rate is remarkably increased, meanwhile, radial and axial three-dimensional mixing of fermentation liquid is achieved, dead volume in the fermentation tank body is eliminated, thalli make more sufficient contact with nutrient substances, and the fermentation efficiency is improved. And secondly, due to the arrangement of the sawtooth plate, a sawtooth-shaped cutting edge forms high-frequency shearing force, bubbles can be efficiently broken, and the phenomenon that the effect is reduced due to material adhesion is not likely to happen.

Description

Technical Field

[0001] This invention relates to the field of bio-fermentation tank technology, specifically to a gas distribution structure for a biopharmaceutical fermentation tank. Background Technology

[0002] In biopharmaceutical fermentation production, the growth and metabolism of aerobic microorganisms require a large amount of dissolved oxygen, i.e., dissolved oxygen, which is present in water. However, oxygen has very low solubility in water. To ensure that the oxygen introduced into the fermentation substrate can be efficiently utilized by microorganisms, researchers have adopted various measures. Initially, some researchers increased the height-to-diameter ratio of the fermenter to prolong the residence time of oxygen within it. However, this method necessitates higher compressed air pressure, leading to increased energy consumption. Later, more researchers used fluid simulation to select agitators with better dispersion effects to increase dissolved oxygen. This significantly improved dissolved oxygen levels, reduced agitation power consumption, and enhanced fermentation production efficiency, becoming a commonly used method while ensuring product quality. In this process, the gas distributor plays a crucial role, directly affecting the air distribution and dispersion within the fermenter.

[0003] In existing technologies, the gas distributors used in fermenters mainly fall into the following categories: single-tube gas distributors, annular porous tube gas distributors, and Venturi jet gas distributors. However, these traditional gas distributors have many shortcomings. For example, single-tube air distributors have poor air dispersion and are not energy-efficient; although annular porous tube distributors and filter-type microbubble distributors allow air to be discharged from small holes or micropores, it quickly re-aggregates and easily causes blockage of the small holes, affecting the distribution effect and even leading to incomplete disinfection and sterilization, resulting in pollution in fermentation production. At the same time, with the development of biochemical technology and the increasing demand for biochemical products, fermenters are gradually developing towards larger and medium-sized models, which places higher demands on the gas-liquid dispersion of gas distributors, requiring them to have better dispersion effect, anti-clogging ability, and energy-saving performance. Summary of the Invention

[0004] The purpose of this invention is to provide a gas distribution structure for a biopharmaceutical fermenter to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a gas distribution structure for a biopharmaceutical fermenter, including a support frame, a fermenter body installed inside the support frame, a control system installed at the front end of the support frame, a protective sleeve fitted on the outside of the fermenter body, three sets of built-in multi-layer sensors installed inside the fermenter body, a bubble breaking mechanism installed inside the fermenter body, and a layered gas distribution mechanism installed on one side of the support frame. The bubble breaking mechanism includes a variable speed motor. A stirring shaft is rotatably installed inside the fermentation tank body. Three sets of guide ring plates are installed on the inner wall of the fermentation tank body. Multiple sets of discs are installed on the outer wall of the stirring shaft. Multiple sets of arc-shaped plates are installed on the outer wall of the multiple sets of discs. Three sets of disturbance plates are installed on the outer wall of each of the three sets of discs. Two sets of serrated plates are installed at both ends of each set of disturbance plates. Concave guide grooves are installed on the inner wall of each of the three sets of guide ring plates.

[0006] Preferably, the lower end of the variable speed motor is fixed to the upper end of the fermenter body, the upper end of the stirring shaft is fixed to the output end of the variable speed motor, every three sets of the disturbance plates are set in the middle of a set of guide ring plates, and the variable speed motor is connected to the control system signal.

[0007] Preferably, the built-in multilayer sensor includes a dissolved oxygen sensor, a pH sensor, a temperature sensor, and a turbidity sensor, and the built-in multilayer sensor is connected to the control system signal.

[0008] Preferably, the layered air distribution mechanism includes a base, three sets of annular pipes and an exhaust pipe. An air source assembly is installed at the upper end of the base. A vertical pipe is installed at the upper opening of the air source assembly. A sterilization filter is installed at the upper end of the air source assembly. Three sets of long pipes are installed through the outer wall of the protective cylinder. A solenoid valve is installed at one end of each of the three sets of long pipes. Multiple air jets are installed on the inner wall of each of the three sets of annular pipes. A concave pipe is installed at the upper end of the sterilization filter.

[0009] Preferably, the base is located on one side of the support frame, the three sets of annular tubes are equidistantly distributed inside the fermenter body and are respectively located above the three sets of guide ring plates, the other end of the three sets of long tubes is fixedly connected to the outer wall of the fermenter body, the protrusion of the three sets of annular tubes is fixedly connected to the inner wall of the fermenter body, and the three sets of annular tubes are connected to the corresponding long tubes.

[0010] Preferably, the exhaust pipe is located on one side of the protective cylinder, the other end of the concave tube is fixed to the upper end of the exhaust pipe, the air source assembly and the sterilization filter are both connected to the control system signal, and the three sets of solenoid valves are all connected to the control system signal.

[0011] Preferably, the outer wall of the exhaust pipe is equipped with two sets of annular seats, and each set of annular pipes has a connecting rod installed on its outer wall.

[0012] Preferably, the protrusions of both sets of annular seats are fixed to the outer wall of the protective cylinder, and the other end of each set of connecting rods is fixed to the inner wall of the fermenter body.

[0013] Preferably, a heat exchange system is provided on the other side of the support frame, two sets of connecting pipes are installed through the outer wall of the protective cylinder, and a spiral heating pipe is installed on the outer wall of the fermenter body.

[0014] Preferably, one end of each of the two sets of connecting pipes is fixed to the two openings of the spiral heating pipe, and the other end of each of the two sets of connecting pipes is fixed to the liquid outlet and liquid inlet of the heat exchange system, respectively. The heat exchange system is connected to the control system via signal.

[0015] Compared with the prior art, the beneficial effects of the present invention are: 1. In this invention, by setting up a bubble breaking mechanism, the diameter of the bubbles can be stably controlled through the synergistic effect of mechanical shearing and flow field guidance. Compared with the traditional structure, the gas-liquid contact area is increased, significantly improving the oxygen dissolution rate. At the same time, it realizes the radial and axial three-dimensional mixing of the fermentation liquid, eliminates the "dead volume" in the fermenter, and makes the contact between the bacteria and nutrients more sufficient, reducing local metabolic differences. Secondly, the setting of the serrated plate, the serrated edge forms a high-frequency shearing force, which can efficiently break the bubbles and is not easily reduced in effect due to material adhesion.

[0016] 2. In this invention, the disturbance plate is designed to create local turbulence during rotation, preventing bubble aggregation and merging; the guide ring plate, with its annular structure, divides the tank into multiple independent flow fields, forcing bubbles to move along a preset path and extending residence time; the concave guide groove guides the bubbles to spiral upward, increasing gas-liquid contact time and preventing bubbles from escaping rapidly along the tank wall; the arc plate generates radial thrust when rotating with the axis, driving the fermentation liquid to flow towards the tank wall and forming a convective circulation with the guide ring plate; multiple sets of discs are distributed along the axis to achieve layered stirring at different heights, adapting to the three-dimensional mixing requirements of large fermenters.

[0017] 3. In this invention, by setting up a layered gas distribution mechanism, "on-demand distribution" is achieved in three independent gas distribution circuits. The intake volume can be dynamically adjusted according to the dissolved oxygen demand at different heights. The setting of the sterilization filter integrates the sterilization filtration and sterile exhaust system to ensure the sterility of the gas entering the system, reduce the risk of fermentation contamination, avoid the "over-transmission" problem of traditional single-path gas supply, improve gas utilization, and reduce compressed air energy consumption.

[0018] 4. In this invention, the bubble breaking mechanism and the layered gas distribution mechanism are connected through the process of "gas supply - breaking - flow guidance" to form a functional closed loop: the layered gas distribution provides a uniform initial gas source, the bubble breaking mechanism refines it into microbubbles, and the flow guidance structure extends the mass transfer time. The three work together to improve the dissolved oxygen efficiency compared with the traditional system, while reducing energy consumption. It is particularly suitable for the large-scale fermentation production of high-aerobic biopharmaceuticals. Attached Figure Description

[0019] Figure 1This is a perspective view of the gas distribution structure of a biopharmaceutical fermenter according to the present invention; Figure 2 This is a partial half-sectional perspective view of the gas distribution structure of a biopharmaceutical fermenter according to the present invention; Figure 3 This is a front view of the gas distribution structure of a biopharmaceutical fermenter according to the present invention; Figure 4 This is a three-dimensional view of a partially sectional fermenter and a bubble breaking mechanism for a biopharmaceutical fermenter according to the present invention. Figure 5 This is a three-dimensional view of a partial bubble breaking mechanism in the gas distribution structure of a biopharmaceutical fermenter according to the present invention; Figure 6 This is a bottom view of the flow guide ring plate of the gas distribution structure of a biopharmaceutical fermenter according to the present invention; Figure 7 This is a schematic diagram of the disturbance plate structure of the gas distribution structure of a biopharmaceutical fermenter according to the present invention; Figure 8 This is a three-dimensional view of the layered gas distribution mechanism of a biopharmaceutical fermenter according to the present invention; Figure 9 This is a system diagram of the gas distribution structure of a biopharmaceutical fermenter according to the present invention.

[0020] In the picture: 1. Support frame; 11. Fermentation tank body; 12. Control system; 13. Protective cylinder; 14. Heat exchange system; 15. Connecting pipe; 16. Spiral heating pipe; 17. Built-in multi-layer sensor; 2. Bubble breaking mechanism; 21. Variable speed motor; 22. Stirring shaft; 23. Guide ring plate; 24. Disc; 25. Arc plate; 26. Disturbance plate; 27. Serrated plate; 28. Concave guide groove; 3. Layered gas distribution mechanism; 31. Base; 32. Gas source assembly; 33. Circular pipe; 331. Connecting rod; 34. Circular seat; 35. Vertical pipe; 36. Exhaust pipe; 37. Sterilization filter; 38. Long pipe; 39. Solenoid valve; 310. Jet nozzle; 311. Concave pipe. Detailed Implementation

[0021] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0022] Example 1: Refer to Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 , Figure 7 , Figure 8 and Figure 9 As shown: A gas distribution structure for a biopharmaceutical fermenter includes a support frame 1, a fermenter body 11 installed inside the support frame 1, a control system 12 installed at the front end of the support frame 1, a protective sleeve 13 covering the outside of the fermenter body 11, three sets of built-in multi-layer sensors 17 installed inside the fermenter body 11, a bubble breaking mechanism 2 installed inside the fermenter body 11, and a layered gas distribution mechanism 3 installed on one side of the support frame 1. The bubble breaking mechanism 2 includes a variable speed motor 21, a stirring shaft 22 is rotatably installed inside the fermentation tank body 11, three sets of guide ring plates 23 are installed on the inner wall of the fermentation tank body 11, multiple sets of discs 24 are installed on the outer wall of the stirring shaft 22, multiple sets of arc plates 25 are installed on the outer wall of the multiple sets of discs 24, three sets of disturbance plates 26 are installed on the outer wall of each of the three sets of discs 24, two sets of serrated plates 27 are installed at both ends of each set of disturbance plates 26, concave guide grooves 28 are installed on the inner wall of each of the three sets of guide ring plates 23, the lower end of the variable speed motor 21 is fixed to the upper end of the fermentation tank body 11, the upper end of the stirring shaft 22 is fixed to the output end of the variable speed motor 21, and every three sets of disturbance plates 26 are set in the middle of a set of guide ring plates 23; The variable speed motor 21 is connected to the control system 12 via signal. The built-in multi-layer sensor 17 includes a dissolved oxygen (DO) sensor, a pH sensor, a temperature sensor, and a turbidity sensor. The built-in multi-layer sensor 17 is connected to the control system 12 via signal.

[0023] In this embodiment, the support frame 1 serves as the load-bearing foundation of the entire system. The fermenter body 11 is fixedly installed inside it, and the front end integrates the control system 12 (core control center). A layered gas distribution mechanism 3 is set on one side, and a heat exchange system 14 is configured on the other side, forming a modular layout that facilitates installation and maintenance. The fermenter body 11 serves as the core fermentation container, and a protective sleeve 13 (which serves both heat preservation and safety protection functions) is fitted on the outside. The bubble breaking mechanism 2 (stirring shaft 22, guide ring plate 23, etc.) is responsible for bubble refinement and flow field optimization. A variable speed motor 21 (installed at the top of the fermenter) provides power to the stirring shaft 22, and the speed is adjusted by the control system 12 (to adapt to the needs of different fermentation stages); multiple sets of discs 24 are fixed to the outer wall of the stirring shaft 22, and arc-shaped plates 25 are installed on the edge of the discs 24 (to push the fermentation liquid to flow radially when rotating with the shaft, thereby enhancing the mixing effect). Each of the three sets of discs 24 has three sets of disturbance plates 26 on its outer wall. The two ends of the plates are provided with serrated plates 27 (made of food-grade stainless steel with a cutting edge angle of 30°). When rotating, the plates break the bubbles to a diameter of 100-300μm through shearing action. The disturbance plates 26 are precisely located in the middle of the three sets of guide ring plates 23, forming a synergistic structure of "rotational crushing + fixed guidance". The inner wall of the guide ring plate 23 (fixed to the inner wall of the tank and parallel to the horizontal plane) is machined with concave guide grooves 28 (evenly distributed along the circumference) to guide the broken bubbles to spiral up along the groove and prolong the gas-liquid contact time. Three sets of guide ring plates 23 are equidistantly distributed, dividing the fermenter body 11 into three independent flow field regions to avoid uneven distribution caused by vertical movement of bubbles; Built-in multi-layer sensor 17 (3 groups distributed along the height direction), including dissolved oxygen (DO) sensor, pH sensor, temperature sensor and turbidity sensor, to collect fermentation parameters of each layer in real time; Dissolved oxygen (DO) sensor: DO probes are installed at different heights to monitor the dissolved oxygen level of each layer in real time (e.g., the dissolved oxygen is higher at the bottom due to aeration, while it may be lower at the top due to bacterial consumption), reflecting the stratified differences in gas-liquid mass transfer and bacterial activity. pH sensor: Multi-layer pH probes can record changes in pH of each layer in real time and detect local metabolic abnormalities in a timely manner (such as a sudden increase in pH caused by the death of bacteria at the bottom). Temperature sensors: Large fermenters may have temperature stratification, and multiple temperature probes can monitor the uniformity of the heating / cooling system; Turbidity sensor: It monitors the concentration of bacteria in each layer in real time through the principle of light scattering, and indirectly reflects the growth status; Precise stratified gas supply reduces ineffective gas consumption. Combined with the low-speed mode of the variable speed motor 21 (which can reduce speed by 30% during the non-logarithmic phase), the overall energy consumption is reduced by 25%. It is suitable for fermenters of different sizes from 500 to 5000L and the parameters can be flexibly adjusted according to the type of microorganism (bacteria, fungi, yeast, etc.), making it highly versatile. The breaking effect of the serrated plate 27 and the disturbance plate 26 increases the gas-liquid contact area. Combined with the spiral flow field design of the concave guide groove 28, the dissolved oxygen rate is improved compared with the traditional structure. The stratified gas distribution and the zoned control of the flow guide ring plate 23 enable the dissolved oxygen deviation in each layer of the fermenter body 11 to be controlled, thus avoiding metabolic abnormalities caused by local hypoxia. Multi-layer sensors monitor and dynamically adjust in real time to ensure stable parameters such as pH and temperature, which is suitable for the fermentation needs of highly sensitive biopharmaceuticals (such as vaccines and recombinant proteins). The modular design allows the bubble breaking mechanism and the layered air distribution components to be disassembled and cleaned separately, meeting GMP cleaning requirements. The control system 12 integrates all operating parameters, supports remote monitoring and data traceability, and reduces the cost of manual intervention.

[0024] Example 2: Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 8 and Figure 9 As shown, the layered gas distribution mechanism 3 includes a base 31, three sets of annular pipes 33, and an exhaust pipe 36. An air source assembly 32 is installed on the upper end of the base 31. A vertical pipe 35 is installed at the upper opening of the air source assembly 32. A sterilization filter 37 is installed at the upper end of the air source assembly 32. Three sets of long pipes 38 are installed through the outer wall of the protective cylinder 13. A solenoid valve 39 is installed at one end of each of the three sets of long pipes 38. Multiple jet nozzles 310 are installed on the inner wall of each of the three sets of annular pipes 33. A concave pipe 311 is installed at the upper end of the sterilization filter 37. The base 31 is located on one side of the support frame 1. The three sets of annular pipes 33 are evenly distributed inside the fermenter body 11 and are located above the three sets of guide ring plates 23. The other ends of the three sets of long pipes 38 are connected to the fermenter body. The outer wall of the main body 11 is fixedly connected. The protrusions of the three sets of annular tubes 33 are all fixedly connected to the inner wall of the fermenter main body 11. The three sets of annular tubes 33 are connected to the corresponding long tubes 38. The exhaust pipe 36 is located on one side of the protective cylinder 13. The other end of the concave tube 311 is fixed to the upper end of the exhaust pipe 36. The gas source component 32 and the sterilization filter 37 are both connected to the control system 12. The three sets of solenoid valves 39 are all connected to the control system 12. Two sets of annular seats 34 are installed on the outer wall of the exhaust pipe 36. A connecting rod 331 is installed on the outer wall of each set of annular tubes 33. The protrusions of the two sets of annular seats 34 are fixed to the outer wall of the protective cylinder 13. The other end of each set of connecting rods 331 is fixed to the inner wall of the fermenter main body 11.

[0025] In this embodiment, the air source assembly 32 (including an air compressor and a flow controller) on the base 31 provides sterile gas, which is then filtered by a sterilization filter 37 (using a 0.22μm filter element) to remove microorganisms and ensure the cleanliness of the incoming air. The concave tube 311 connects the sterilization filter 37 and the exhaust pipe 36 to achieve sterile discharge of excess gas, and the annular seat 34 fixes the position of the exhaust pipe 36 to ensure exhaust stability. Three sets of circular tubes 33 (equally distributed inside the tank, located above the guide ring plate 23) are fixed to the inner wall of the tank by connecting rods 331. Twenty jet nozzles 310 are evenly installed on the inner wall to prevent liquid backflow. Three sets of long pipes 38 penetrate the tank wall and the protective cylinder 13, with one end connected to the circular pipe 33 and the other end installed with a solenoid valve 39 (which independently controls the air intake of each layer through the control system 12) to achieve dynamic adjustment of "more air intake at the bottom layer and less air intake at the top layer" (e.g., the air intake of the bottom layer is 50% higher than that of the top layer during the logarithmic fermentation stage). The sterilization filter 37 and the aseptic exhaust design reduce the risk of contamination, allowing the rate of bacterial contamination to be controlled.

[0026] Example 3: Figure 1 , Figure 2 , Figure 3 and Figure 9 As shown, a heat exchange system 14 is provided on the other side of the support frame 1. Two sets of connecting pipes 15 are installed through the outer wall of the protective cylinder 13. A spiral heating pipe 16 is installed on the outer wall of the fermenter body 11. One end of the two sets of connecting pipes 15 is fixed to the two openings of the spiral heating pipe 16 respectively. The other end of the two sets of connecting pipes 15 is fixed to the liquid outlet and liquid inlet of the heat exchange system 14 respectively. The heat exchange system 14 is connected to the control system 12 via signal.

[0027] In this embodiment, the heat exchange system 14 forms a closed loop with the spiral heating pipe 16 on the outer wall of the fermenter through two sets of connecting pipes 15, and heat transfer oil or constant temperature water is introduced; in conjunction with the built-in temperature sensor, it can achieve precise control of fermentation temperature (e.g., 37℃±0.5℃) to avoid microbial inactivation due to temperature fluctuations. Built-in multi-layer sensors 17 collect dissolved oxygen, pH, temperature and turbidity data of each layer in real time and transmit them to the control system 12. The control system 12 analyzes the data through algorithms and automatically adjusts the speed of the variable speed motor 21, the opening degree of the solenoid valve 39 and the power of the heat exchange system 14 to form a closed-loop control of "monitoring-analysis-execution".

[0028] The working principle of this invention is as follows: First, before fermentation, the control system 12 receives preset parameters (such as target temperature 37℃, dissolved oxygen threshold 30%~50%, pH range 6.5-7.2) and completes equipment self-check. The heat exchange system 14 is started, and the heat transfer medium is introduced into the spiral heating pipe 16 through the connecting pipe 15 to preheat the fermenter body 11 to the set temperature. The gas source component 32 undergoes an airtightness test, and the sterilization filter 37 initiates a self-cleaning program to ensure that the gas passage is sterile. Built-in multi-layer sensor with 17 calibrations ensures accurate detection of parameters such as dissolved oxygen, pH, and temperature; Then, the compressed air generated by the air source assembly 32 (air compressor) first enters the sterilization filter 37, where microorganisms, dust, and other impurities are removed through a 0.22μm filter element. The purified sterile gas is then divided into two paths: The mainstream gas enters the three sets of long pipes 38 through the vertical pipe 35; Excess gas is introduced into the exhaust pipe 36 through the concave tube 311 and is stably discharged by the exhaust pipe 36 fixed by the annular seat 34, thus avoiding excessive pressure inside the tank. Layered precision gas supply: The control system 12 adjusts the opening degree of the three sets of solenoid valves 39 according to the fermentation stage (such as the initial stage, logarithmic stage, and stationary stage): In the early stage of fermentation (cell adaptation period): the air intake of the bottom ring tube 33 accounts for 40%, the middle layer 30%, and the top layer 30%, to avoid gas waste; Logarithmic phase (high aerobic stage): The air intake of the bottom layer is increased to 60%, the middle layer to 25%, and the top layer to 15%. Gas is injected into the fermentation liquid through the jet nozzle 310 (tilted 45° downwards), and the gas impacts the liquid surface to form initial bubbles. The annular tube 33 is fixed to the inner wall of the tank by the connecting rod 331 to ensure the stability of the jet position and to form a spatial fit with the guide ring plate 23. Level 1 fragmentation and disturbance: The variable speed motor 21 drives the stirring shaft 22 to rotate (speed 100-300 rpm), which in turn drives the disc 24 and the disturbance plate 26 on the outer wall to rotate. The serrated plates 27 (30° cutting edge) at both ends of the disturbance plate 26 cut the bubble at high speed, breaking the initial bubble (1-3 mm in diameter) to 500-800 μm; The arc-shaped plate 25 rotates with the disc 24, pushing the fermentation liquid to form a radial circulation, allowing the bubbles to initially diffuse; Secondary refinement and diversion: The broken bubbles rise to the area of ​​the guide ring plate 23, where the concave guide groove 28 (U-shaped cross-section) guides the bubbles to rise along a spiral path. The flow guide ring plate 23 divides the tank into three independent flow fields to prevent bubbles from moving vertically. The spiral upward path extends the bubble residence time by more than 30%, ensuring full contact with the fermentation broth, further refining the bubbles to 100-300μm, and increasing the gas-liquid contact area by more than 5 times. Finally, full-can blend fortification: The multi-layered discs 24 and the guide ring plates 23 are alternately distributed to form a cycle of "stirring-guiding-re-stirring". After the lower-level bubbles are broken by the disturbance plate 26, they are guided to the middle layer by the lower-level guide ring plate 23; The bubbles in the middle layer are broken up again under secondary stirring and enter the upper layer through the middle layer guide ring plate 23, ultimately achieving uniform distribution of bubbles throughout the tank. Real-time data acquisition: Three sets of built-in multi-layer sensors 17 (located in the upper, middle, and lower layers respectively) collect data per second: Dissolved oxygen sensors monitor the oxygen concentration in each layer to determine if there is local hypoxia. pH sensor provides feedback on changes in metabolic acidity and alkalinity (such as pH decrease caused by bacterial acid production). Temperature sensors track changes in heat in the fermentation broth (heat generated by microbial metabolism or heat dissipation from the environment). Intelligent feedback adjustment: The control system 12 compares the real-time data with the preset threshold and automatically executes the adjustment command: If the dissolved oxygen in the bottom layer is below 30%, increase the opening of the bottom layer solenoid valve 39 and increase the speed of the variable speed motor 21 (to increase crushing efficiency). If the pH of the middle layer is below 6.5: Add alkali solution to the feed system (not shown) and reduce the air intake in the corresponding area (to reduce CO2 dissolution). Temperature deviation of 37℃±0.5℃: Instruct the heat exchange system 14 to adjust the flow rate of the heat transfer medium and balance the temperature through the spiral heating tube 16; The heat exchange system 14 forms a closed loop with the spiral heating tube 16 through two sets of connecting pipes 15: When the sensor detects that the temperature is too high (e.g., >38℃): Low-temperature heat transfer oil is introduced, and heat is absorbed through the spiral tube; If the temperature is too low (e.g., <36℃): switch to a high-temperature medium to release heat and maintain a constant temperature; The protective casing 13 reduces heat exchange between the tank wall and the outside environment, helping to maintain temperature stability.

[0029] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A gas distribution structure for a biopharmaceutical fermenter, comprising a support frame (1), characterized in that: The fermenter body (11) is installed inside the support frame (1), and a control system (12) is installed at the front end of the support frame (1). A protective sleeve (13) is fitted on the outside of the fermenter body (11). Three sets of built-in multi-layer sensors (17) are installed inside the fermenter body (11). A bubble breaking mechanism (2) is installed inside the fermenter body (11). A layered gas distribution mechanism (3) is installed on one side of the support frame (1). The bubble breaking mechanism (2) includes a variable speed motor (21). A stirring shaft (22) is rotatably installed inside the fermentation tank body (11). Three sets of guide ring plates (23) are installed on the inner wall of the fermentation tank body (11). Multiple sets of discs (24) are installed on the outer wall of the stirring shaft (22). Multiple sets of arc plates (25) are installed on the outer wall of the multiple sets of discs (24). Three sets of disturbance plates (26) are installed on the outer wall of each of the three sets of discs (24). Two sets of serrated plates (27) are installed at both ends of each set of disturbance plates (26). Concave guide grooves (28) are installed on the inner wall of each of the three sets of guide ring plates (23). The layered air distribution mechanism (3) includes a base (31), three sets of annular pipes (33) and an exhaust pipe (36). An air source assembly (32) is installed on the upper end of the base (31). A vertical pipe (35) is installed at the upper opening of the air source assembly (32). A sterilization filter (37) is installed at the upper end of the air source assembly (32). Three sets of long pipes (38) are installed through the outer wall of the protective cylinder (13). A solenoid valve (39) is installed at one end of each of the three sets of long pipes (38). Multiple sets of jet nozzles (310) are installed on the inner wall of each of the three sets of annular pipes (33). A concave pipe (311) is installed at the upper end of the sterilization filter (37).

2. The gas distribution structure of a biopharmaceutical fermenter according to claim 1, characterized in that: The lower end of the variable speed motor (21) is fixed to the upper end of the fermentation tank body (11), the upper end of the stirring shaft (22) is fixed to the output end of the variable speed motor (21), and every three sets of the disturbance plates (26) are set in the middle of a set of guide ring plates (23). The variable speed motor (21) is connected to the control system (12) via signal.

3. The gas distribution structure of a biopharmaceutical fermenter according to claim 1, characterized in that: The built-in multilayer sensor (17) includes a dissolved oxygen (DO) sensor, a pH sensor, a temperature sensor and a turbidity sensor, and the built-in multilayer sensor (17) is signal-connected to the control system (12).

4. The gas distribution structure of a biopharmaceutical fermenter according to claim 1, characterized in that: The base (31) is located on one side of the support frame (1). The three sets of circular tubes (33) are evenly distributed inside the fermenter body (11) and are located above the three sets of guide ring plates (23). The other end of the three sets of long tubes (38) is fixedly connected to the outer wall of the fermenter body (11). The protrusions of the three sets of circular tubes (33) are fixedly connected to the inner wall of the fermenter body (11), and the three sets of circular tubes (33) are connected to the corresponding long tubes (38).

5. The gas distribution structure of a biopharmaceutical fermenter according to claim 4, characterized in that: The exhaust pipe (36) is located on one side of the protective cylinder (13), and the other end of the concave tube (311) is fixed to the upper end of the exhaust pipe (36). The air source assembly (32) and the sterilization filter (37) are both connected to the control system (12) by signal. The three sets of solenoid valves (39) are all connected to the control system (12) by signal.

6. The gas distribution structure of a biopharmaceutical fermenter according to claim 5, characterized in that: The outer wall of the exhaust pipe (36) is equipped with two sets of annular seats (34), and each set of annular pipes (33) is equipped with a connecting rod (331) on its outer wall.

7. The gas distribution structure of a biopharmaceutical fermenter according to claim 6, characterized in that: The protrusions of both sets of ring seats (34) are fixed to the outer wall of the protective cylinder (13), and the other end of each set of connecting rods (331) is fixed to the inner wall of the fermenter body (11).

8. The gas distribution structure of a biopharmaceutical fermenter according to claim 1, characterized in that: A heat exchange system (14) is provided on the other side of the support frame (1), two sets of connecting pipes (15) are installed through the outer wall of the protective cylinder (13), and a spiral heating pipe (16) is installed on the outer wall of the fermenter body (11).

9. The gas distribution structure of a biopharmaceutical fermenter according to claim 8, characterized in that: One end of each of the two sets of connecting pipes (15) is fixed to the two ends of the spiral heating pipe (16), and the other end of each of the two sets of connecting pipes (15) is fixed to the liquid outlet and liquid inlet of the heat exchange system (14), and the heat exchange system (14) is connected to the control system (12) via signal.

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