Separation device for extracting serum proteins

Abstract

The present application relates to the technical field of separation and extraction, and particularly relates to a separation device for serum protein extraction, which comprises a reaction tank and a stirring rod, the stirring rod is provided with stirring blades rotating with the stirring rod, the reaction tank is fixedly provided with a stirring motor, and the separation device further comprises: a driving shaft vertically arranged in the reaction tank and deviated from the central position of the reaction tank, the stirring motor is used for driving the driving shaft to rotate; a mounting frame comprising a first connecting part and a second connecting part, the first connecting part is sleeved on the driving shaft and rotationally connected with the driving shaft, and the second connecting part is used for rotationally mounting the stirring rod; a transmission assembly is arranged between the driving shaft and the stirring rod, the stirring rod is driven to rotate by the driving shaft; and a driving assembly is used for driving the first connecting part to rotate, so that the stirring rod swings around the driving shaft. The stirring rod is stirred during swinging in the reaction tank, the uniformity of mixing is improved, and the problem of local over-acidification is reduced.

Description

Technical Field

[0001] This invention relates to the technical field of separation and extraction, and specifically to a separation device for extracting serum proteins. Background Technology

[0002] Human serum is a complex mixture containing various high-value bioactive proteins. Serum albumin, the most abundant protein in plasma, plays a vital physiological role in maintaining osmotic pressure and transporting various substances. Immunoglobulins are the main components of antibodies, playing important roles in immune defense and therapy. Therefore, the efficient and high-purity separation of these target proteins from human serum is crucial for ensuring drug safety, efficacy, and large-scale production. Precipitation is a common method for separating target proteins. Precipitation utilizes the significant differences in solubility of target proteins in different solvents. By adding a precipitating agent, non-target proteins or the target protein itself precipitate from the solution, forming a solid phase, thus achieving separation from the liquid phase. To ensure rapid, thorough, and uniform mixing of the added precipitating agent with the serum solution, avoiding excessively high or low local concentrations that could lead to incomplete or non-specific precipitation, stirring is necessary during the addition of the precipitating agent to promote the interaction between the precipitating agent and protein molecules, accelerating the precipitation process.

[0003] Chinese patent CN112569645B discloses a rapid plasma component separation system, including a reaction vessel with a stirring mechanism and a liquid addition pipe. The liquid addition pipe has a liquid addition valve. The stirring mechanism includes a stirring rod inside the reaction vessel, with stirring blades fixedly mounted at its lower end. The upper end of the stirring rod passes through the reaction vessel and is coaxially and fixedly connected to the output shaft of a drive motor. The stirring rod and the reaction vessel are connected by a sealed bearing. The drive motor is fixedly connected to the reaction vessel. The stirring rod is located at the center of the reaction vessel, which has a plasma inlet. Acid is added to the reaction vessel through an opening in the liquid addition pipe. The drive motor rotates the stirring rod, which in turn drives the stirring blades to mix the acid and plasma.

[0004] However, the stirring rod is located at the center of the reaction vessel, and its rotation primarily generates axial and radial flow fields through the stirring blades. Since plasma separation processes require maintaining slow and gentle stirring intensity to avoid disrupting the formed flocculent precipitate, this centrosymmetric flow field has inherent limitations. In areas far from the stirring blades, such as near the vessel wall, low-shear, weak-circulation mixing dead zones easily form. When acid is injected, the acid diffuses slowly within these dead zones, exposing plasma proteins in these areas to an overly acidic environment, increasing the risk of protein denaturation or inactivation, ultimately affecting the activity and yield of the separated components. Summary of the Invention

[0005] This invention provides a separation device for serum protein extraction, which aims to solve the problem in related technologies where the stirring rod is located in the center of the reaction vessel, making rapid mixing difficult and causing local over-acidity.

[0006] A separation device for serum protein extraction according to the present invention includes a reaction vessel and a stirring rod disposed inside the reaction vessel. The stirring rod is equipped with stirring blades that rotate with the stirring rod. A stirring motor is fixedly disposed on the reaction vessel. The device further includes: a drive shaft, vertically disposed inside the reaction vessel and offset from the center of the reaction vessel, for which the stirring motor drives the drive shaft to rotate; a mounting bracket, including a first connecting part and a second connecting part, the first connecting part being sleeved on the drive shaft and rotatably connected to the drive shaft, and the second connecting part being rotatably mounted on the stirring rod; the first connecting part and the second connecting part being spaced apart and fixedly connected relative to each other; a transmission assembly, installed between the drive shaft and the stirring rod, for driving the stirring rod to rotate via the drive shaft; and a drive assembly for driving the first connecting part to rotate, causing the stirring rod to oscillate around the drive shaft.

[0007] Its effects are as follows: By setting a drive shaft offset from the center of the reaction vessel, and installing a mounting bracket between the drive shaft and the stirring rod, a transmission assembly is used to drive the stirring rod and the drive shaft, achieving a compound planetary motion mode for the stirring rod. This means the stirring rod simultaneously rotates on its own axis and oscillates around the drive shaft. This asymmetrical and multi-dimensional stirring trajectory allows for a larger stirring area within the reaction vessel, while also generating turbulence and vortices in the liquid. The oscillating revolution of the stirring rod greatly expands the stirring area and significantly reduces the dead zone of fluid mixing at the edge of the reaction vessel. The synergistic effect of these two factors fundamentally overcomes the inherent defects of traditional central stirring, ensuring that acid or other reagents are rapidly and uniformly dispersed in the serum protein solution. This effectively reduces the problem of excessive acidity caused by localized high concentrations, greatly improving reaction uniformity, protein extraction efficiency, and product quality stability.

[0008] Preferably, the first connecting part is cylindrical and a spiral driving groove is provided on the inner wall of the first connecting part. The driving assembly includes a vertically reciprocating slider, and the outer wall of the slider has a protrusion that slides in the driving groove.

[0009] Its advantages are: the sliding engagement between the slider and the helical groove ensures the stability of the motion trajectory, maintaining smooth operation even under reciprocating or frequent start-stop conditions, thus significantly improving the durability and long-term reliability of the device. At the same time, the structure is relatively simple, easy to manufacture, assemble, and maintain.

[0010] Preferably, a reciprocating screw is coaxially arranged on the drive shaft. The reciprocating screw rotates with the drive shaft. The slider is vertically guided by an anti-rotation frame fixed on the reaction vessel. The reciprocating screw cooperates with the slider. The unidirectional rotation of the reciprocating screw is used to drive the slider to perform vertical reciprocating movement.

[0011] Its effect is that when the drive shaft maintains a constant rotation direction, the reciprocating screw can automatically and continuously drive the slider to complete the vertical reciprocating stroke. The anti-rotation frame fixed to the tank ensures that the slider moves along the axial direction, thereby ensuring the power transmission between the drive tank and the slider, and thus determining the repeatability and stability of the oscillating revolution trajectory of the stirring rod.

[0012] Preferably, the ratio of the pitch of the reciprocating lead screw to the pitch of the drive slot is 1:200-500.

[0013] Its effect is that when the slider moves in a low-speed linear motion, this motion is transmitted to the spiral drive groove, which allows the stirring rod to swing smoothly around the drive shaft with almost no inertial impact, thereby avoiding the vibration, noise and wear problems inherent in reciprocating mechanisms.

[0014] Preferably, the transmission assembly includes a driving bevel gear, a driven bevel gear, and a connecting bevel gear. There are two connecting bevel gears, which are coaxially fixed. The first connecting part and the second connecting part are fixed by a hollow crossbar. The connecting bevel gears are rotatably disposed inside the hollow crossbar. The driving bevel gear is coaxially fixed on the drive shaft, and the driven bevel gear is coaxially fixed on the stirring rod. One connecting bevel gear meshes with the driving bevel gear, and the other connecting bevel gear meshes with the driven bevel gear.

[0015] Its effect is that the meshing of the driving bevel gear, driven bevel gear, and connecting bevel gear can still transmit the power of the drive shaft to the stirring rod through precise meshing in the dynamic environment of revolution and oscillation, ensuring the stability of the rotation speed of the stirring blade.

[0016] Preferably, the stirring rod has a hollow structure inside and its upper end is connected to the top of the reaction vessel. An air vent is provided on the back surface of the stirring blade, and the air vent is located on the stirring blade away from the stirring rod. A gas channel is provided inside the stirring blade, and the air vent is connected to the hollow part inside the stirring rod through the gas channel. A sealing component is provided at the position of the air vent, which is used to open or close the air vent.

[0017] Its effect is as follows: during the rotation of the stirring blades, when the low-pressure zone is formed on the back surface of the stirring blades, the gas at the top of the tank can be drawn into the vent through the gas channel, thus eliminating the mechanical damage to proteins caused by cavitation bubbles forming on the back surface of the stirring blades. At the same time, the bubbles formed after venting can also play a role in air lifting, further improving mixing efficiency.

[0018] Preferably, the sealing assembly includes an outer sleeve, a reset member, and a sealing body. The outer sleeve is fixed inside the gas channel, and a vent hole for connecting to the gas outlet is formed on the outer wall of the outer sleeve. The sealing body is slidably fitted inside the outer sleeve, and the two ends of the sealing body along the moving area of ​​the outer sleeve are the initial position and the working position, respectively. When the sealing body is in the initial position, the sealing body blocks the vent hole; when the sealing body is in the working position, the sealing body opens the vent hole. The reset member is used to control the sealing body to be in the initial position or the working position.

[0019] Its advantages are: the sealing body, reset component, and outer sleeve can be installed into the gas channel in one go, making manufacturing convenient and quick. The vent hole on the outer sleeve is connected to the outlet hole. The opening or closing of the vent hole is controlled by the movement of the sealing body, thereby controlling the outlet hole. This allows the outer sleeve and the sealing body to adopt a mutually sealing fit structure, reducing the requirements for the machining precision of the gas channel.

[0020] Preferably, the reset element is a tension spring, with one end fixed to the sealing body and the other end fixed to the outer sleeve. At the same time, a magnet is fixedly provided at the end of the outer sleeve away from the working position of the sealing body. The magnet is used to attract the sealing body, and the reset element and the magnet together keep the sealing body in the initial position.

[0021] The effect is as follows: the combination of the tension spring and the magnet requires the sealing body to overcome a large centrifugal force when leaving its initial position, which typically occurs when the stirring blade rotates at a high speed. When the sealing body returns to its original position, it is mainly subjected to the tension of the reset element, at which point the attraction force of the magnet on the sealing body is relatively small. Therefore, during the resetting process, the rotational speed of the stirring blade will be lower than the speed required for the sealing body to move to its working position. This ensures that the stirring blade maintains a stable state during operation and avoids frequent movement near the critical speed.

[0022] Preferably, the gas channel is located inside the stirring blade and parallel to the stirring blade. The end of the gas channel away from the stirring rod opens at the edge of the stirring blade. The outer sleeve is inserted into the gas channel from the opening and fixed at the position where the vent and the outlet are connected.

[0023] Its effect is that the end of the gas channel away from the stirring rod has an opening at the edge of the stirring blade, which makes it easy to open the gas channel and facilitates the installation of the outer sleeve into the gas channel.

[0024] Preferably, a stirring paddle fixed to a stirring rod is disposed below the stirring blade, the stirring rod is plate-shaped and arranged radially along the stirring rod, and the surface of the stirring blade is inclined to the axis of the stirring rod.

[0025] By adopting the above technical solution, the beneficial effects of the present invention are as follows:

[0026] This invention utilizes a stirring rod that simultaneously rotates and oscillates around itself. This asymmetrical and multi-dimensional stirring trajectory allows for a wider stirring range within the reaction vessel. The oscillation and revolution of the stirring rod significantly expands the stirring area and substantially reduces the dead zones at the edges of the reaction vessel. The synergy of these two processes ensures that acid or other reagents are rapidly and uniformly dispersed into the serum protein solution, effectively reducing the problem of excessive acidity caused by localized high concentrations. During the rotation of the stirring blades, when a low-pressure zone forms on the back surface of the blades, allowing gas from the top of the vessel to be drawn into the vent through the gas channel, mechanical damage to proteins caused by cavitation on the back surface of the stirring blades can be eliminated. When the slider moves at low speed in a straight line, this motion is transmitted to the helical drive groove, enabling the stirring rod to oscillate smoothly around the drive shaft with almost no inertial impact. Attached Figure Description

[0027] Figure 1 This is a schematic diagram of the external shape of a separation device for serum protein extraction according to the present invention;

[0028] Figure 2 This is a schematic diagram of the internal structure of the reaction vessel in an embodiment of the present invention;

[0029] Figure 3 This is a schematic diagram of the mounting bracket in an embodiment of the present invention;

[0030] Figure 4 yes Figure 2 A cross-sectional view along the AA direction;

[0031] Figure 5 This is a schematic diagram of the slider mounting structure in an embodiment of the present invention;

[0032] Figure 6 This is a schematic diagram of the assembly position of the connecting shell and the anti-rotation frame in an embodiment of the present invention;

[0033] Figure 7 This is a schematic diagram of the installation structure of the transmission assembly in an embodiment of the present invention;

[0034] Figure 8 This is a schematic diagram showing the installation position of the stirring blades in an embodiment of the present invention;

[0035] Figure 9 This is a schematic diagram of the gas passage inside the stirring blade in an embodiment of the present invention;

[0036] Figure 10 yes Figure 9 Cross-sectional view along the BB direction;

[0037] Figure 11 This is a schematic diagram of the sealing component in an embodiment of the present invention.

[0038] Figure label:

[0039] 1. Reaction vessel; 11. Stirring motor; 12. Drive shaft; 13. Connecting shell; 131. Positioning hole; 2. Stirring rod; 3. Mounting bracket; 31. First connecting part; 311. Drive groove; 32. Second connecting part; 33. Crossbar; 4. Transmission assembly; 41. Driving bevel gear; 42. Connecting bevel gear; 43. Driven bevel gear; 5. Stirring blade; 51. Vent hole; 52. Gas passage; 6. Drive assembly; 61. Reciprocating screw; 62. Slider; 621. Protrusion; 622. Guide hole; 7. Anti-rotation bracket; 71. Upper mounting ring; 72. Lower mounting ring; 73. Guide rod; 8. Stirring paddle; 9. Sealing assembly; 91. Outer sleeve; 911. Vent hole; 92. Reset component; 93. Sealing body; 94. Magnet. Detailed Implementation

[0040] The following is combined Figures 1 to 11 Embodiments of the present invention will be described in detail below, examples of which are illustrated in the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain the present invention, and should not be construed as limiting the present invention.

[0041] Example 1

[0042] This embodiment discloses a separation device for serum protein extraction, such as... Figure 1 and Figure 2 As shown, the device includes a reaction vessel 1, which is cylindrical and vertically arranged. A manhole and a liquid inlet are located at the top of the reaction vessel 1, with the liquid inlet requiring a connecting pipe. The manhole is positioned off-center from the top of the reaction vessel 1. A stirring motor 11 is also fixedly mounted at the top of the reaction vessel 1, located away from the manhole. Inside the reaction vessel 1, a stirring rod 2 and a drive shaft 12 are arranged. Both the drive shaft 12 and the stirring rod 2 are vertically arranged, with the drive shaft 12 rotatably mounted on the reaction vessel 1. The stirring rod 2 and the drive shaft 12 are connected via a mounting bracket 3, which is rotatably mounted around the axis of the drive shaft 12. The drive shaft 12 is rotatably mounted on the mounting bracket 3. During operation, the drive shaft 12 drives the stirring rod 2 to rotate via the transmission assembly 4. At the same time, the mounting bracket 3 can also swing around the axis of the drive shaft 12, thereby enabling the stirring blades 5 mounted on the stirring rod 2 to generate a complex circulating flow field in the reaction tank 1. The movement trajectory of the stirring blades 5 can also cover a larger area in the reaction tank 1, allowing the plasma and acid in the reaction tank 1 to mix more quickly and reducing the mixing dead zone.

[0043] refer to Figure 2 and Figure 3The mounting bracket 3 includes a first connecting part 31 and a second connecting part 32. The first connecting part 31 is cylindrical and sleeved on the outside of the drive shaft 12. The upper end of the drive shaft 12 is fixedly connected to the output shaft of the stirring motor 11, and the lower end of the drive shaft 12 is rotatably mounted on the bottom of the reaction vessel 1. The upper end of the first connecting part 31 extends from the inside of the reaction vessel 1 to the outside of the reaction vessel 1 and is rotatably connected to the reaction vessel 1 via a bearing. A connecting shell 13 is provided on the upper end of the first connecting part 31. The connecting shell 13 is fixed to the vessel body and is used to fix the stirring motor 11, so that the drive shaft 12 is also located inside the connecting shell 13, ensuring the sealing of the reaction vessel 1. The second connecting part 32 is rotatably connected to the stirring rod 2 via a bearing. The second connecting part 32 can also be cylindrical, and the lower end of the second connecting part 32 is sealed to the outer wall of the stirring rod 2 by a sealing ring. The bearing is located inside the second connecting part 32. The stirring rod 2 passes through the center of the second connecting part 32, and its upper end protrudes from the upper end of the second connecting part 32, so that the upper end of the stirring rod 2 is close to the inner top wall of the reaction vessel 1. The upper end of the stirring rod 2 is always positioned above the liquid surface inside the reaction vessel 1. The first connecting part 31 and the second connecting part 32 are fixedly connected by a crossbar 33, and the two ends of the crossbar 33 are fixed to the side walls of the first connecting part 31 and the second connecting part 32, respectively.

[0044] refer to Figure 3 , Figure 4 and Figure 5 The lower end of the first connecting part 31 is rotatably connected to the drive shaft 12 via a bearing and is sealed with a sealing ring. A drive assembly 6 for driving the first connecting part 31 is disposed inside the first connecting part 31, and the drive assembly 6 is connected to the connecting shell 13. In other embodiments, the drive assembly 6 can be driven independently by a motor. In this embodiment, the drive assembly 6 is connected to the drive shaft 12, causing the drive shaft 12 to drive the first connecting part 31 to rotate via the drive assembly 6. A spiral drive groove 311 is formed on the inner wall of the first connecting part 31 from top to bottom. The drive assembly 6 includes a reciprocating screw 61 and a slider 62 that cooperates with the reciprocating screw 61. When the reciprocating screw 61 rotates in one direction, the slider 62 automatically moves up and down on the reciprocating screw 61. The reciprocating screw 61 and the slider 62 are connected using a conventional method. In this embodiment, the outer side of the slider 62 has a protrusion 621, which is used to fit into the drive groove 311. During the reciprocating movement of the slider 62, the protrusion 621 slides along the drive groove 311. The angle of the spiral shape of the drive groove 311 is the angle at which the stirring blade 5 swings around the drive shaft 12 (reference). Figure 4The swing angle (within the range indicated by the dashed arrow) can be selected as 30-90 degrees in this embodiment. The reciprocating screw 61 is sleeved on the drive shaft 12 and connected to it via a key, so that when the drive shaft 12 rotates, the reciprocating screw 61 rotates synchronously with the drive shaft 12. An anti-rotation frame 7 is connected to the slider 62 and is mounted on the connecting shell 13.

[0045] To enable the drive shaft 12 to simultaneously drive the stirring rod 2 to rotate around its own axis and also to revolve the stirring rod 2 around the axis of the drive shaft 12, allowing the stirring rod 2 to complete one oscillation after multiple rotations, the ratio of the pitch of the reciprocating lead screw 61 to the pitch of the drive groove 311 can be set to 1:200-500. (Reference) Figure 5 and Figure 6 The anti-rotation frame 7 includes an upper mounting ring 71, a lower mounting ring 72, and three guide rods 73. Three guide holes 622 are provided on the slider 62, and the guide rods 73 slide in a corresponding manner with each guide hole 622. The upper mounting ring 71 is located at the upper end of the three guide rods 73, and the lower mounting ring 72 is located at the lower end of the three guide rods 73. The guide rods 73 are threadedly connected to the upper mounting ring 71, with the upper end of the guide rod 73 passing through the upper mounting ring 71, and the lower end of the guide rod 73 fixed by bolts. When the slider 62 is connected to the three guide rods 73, it is positioned between the upper mounting ring 71 and the lower mounting ring 72. The upper mounting ring 71 and the lower mounting ring 72 simultaneously improve the stability of the three guide rods 73. Three positioning holes 131 are provided on the connecting shell 13, and the upper ends of the guide rods 73 are inserted into these three positioning holes 131. The upper mounting ring 71 is supported by the upper end of the first connecting part 31, forcing the upper end of the guide rod 73, located above the upper mounting ring 71, to be inserted into the positioning hole 131, thereby connecting the connecting shell 13 to the anti-rotation frame 7. When the drive shaft 12 rotates, the drive shaft 12 drives the reciprocating screw 61 to rotate. The anti-rotation frame 7 can prevent the slider 62 from rotating with the reciprocating screw 61. The slider 62 moves vertically along the guide rod 73, and then the slider 62 drives the first connecting part 31 to rotate horizontally, realizing the revolution of the stirring rod 2 around the drive shaft 12.

[0046] refer to Figure 7 The transmission assembly 4 includes a driving bevel gear 41, a driven bevel gear 43, and a connecting bevel gear 42. There are two connecting bevel gears 42, which are coaxially fixed and rotatably mounted inside the hollow crossbar 33. The driving bevel gear 41 is coaxially fixed to the drive shaft 12, and the driven bevel gear 43 is coaxially fixed to the stirring rod 2. One connecting bevel gear 42 meshes with the driving bevel gear 41, and the other connecting bevel gear 42 meshes with the driven bevel gear 43. When the drive shaft 12 rotates, it can drive the stirring rod 2 to revolve around the drive shaft 12, and it can also rotate on its own axis via the driving bevel gear 41, the two connecting bevel gears 42, and the driven bevel gear 43.

[0047] Example 2

[0048] The difference from Example 1 is that, referring to Figure 7 and Figure 8 The stirring rod 2 has a hollow internal structure. Its upper end connects to the interior of the reaction vessel 1. A connecting hole is provided on the side wall where the stirring blade 5 is mounted on the stirring rod 2. An vent 51 is provided on the stirring blade 5, located on its back surface relative to its front surface. During the rotation of the stirring blade 5, the front surface agitates the plasma within the reaction vessel 1. The vent 51 is positioned away from the stirring rod 2, and the connecting hole communicates with it. Because the linear velocity of the stirring blade 5 is higher when it is further away from the stirring rod 2, cavitation is more likely to occur on its back surface during rotation. These cavitation bubbles can then rupture due to pressure, causing mechanical damage to the proteins in the plasma and reducing the protein product yield. When the vent 51 is positioned on the backwater surface, a low-pressure zone is formed on the backwater surface of the stirring blades 5. This causes gas to flow from the upper end of the stirring rod 2 towards the vent 51, filling the low-pressure zone and reducing cavitation. This reduces damage to proteins and improves the activity and yield of the separated components. Simultaneously, as the gas flows from the vent 51 into the plasma, it forms bubbles. Due to their low density, these bubbles gradually move upwards during stirring, causing the plasma to churn upwards, creating a lift effect and further enhancing the mixing efficiency.

[0049] refer to Figure 8 Below the stirring blade 5, a stirring paddle 8 is fixed on the stirring rod 2. The stirring paddle 8 is plate-shaped and arranged radially along the stirring rod 2. In this embodiment, the number of stirring paddles 8 is set to two and they are evenly arranged along the circumference of the stirring rod 2. The main function of the stirring paddle 8 is to make the plasma flow radially. The surface of the stirring blade 5 is inclined to the axis of the stirring rod 2, and its main function is to make the plasma flow axially. Thus, the stirring blade 5 and the stirring paddle 8 can further improve the mixing efficiency of the plasma.

[0050] refer to Figure 9 and Figure 10When the stirring blade 5 is not rotating or its rotation speed is low, the back surface of the stirring blade 5 will not generate cavitation bubbles due to the timely filling of plasma, and the low-pressure zone will not be fully formed. At this time, in order to prevent plasma from entering the vent hole 51, a sealing component 9 for sealing the vent hole 51 is provided inside the stirring blade 5. The sealing component 9 is inside the stirring blade 5. When the speed of the stirring blade 5 increases, the sealing component 9 opens the vent hole 51 again. In this embodiment, a gas channel 52 is provided parallel to the surface of the stirring blade 5 and located inside the stirring blade 5. One end of the gas channel 52 is connected to the connecting hole, and the other end opens from the edge of the stirring blade 5, making it convenient to insert the sealing component 9 into the gas channel 52. The sealing component 9 can be fixed in the gas channel 52 by interference fit or by adhesive. The opening of the gas channel 52 at the edge of the stirring blade 5 needs to be sealed, and the sealing method can be screws or plugs. The vent hole 51 is connected to the gas channel 52 from the side wall of the gas channel 52.

[0051] refer to Figure 11 The sealing assembly 9 includes an outer sleeve 91, a reset element 92, and a sealing body 93. The sealing body 93 is located inside the outer sleeve 91 and seals against the inner wall of the outer sleeve 91. The sealing body 93 is slidably connected to the outer sleeve 91. The movement of the sealing body 93 within the outer sleeve 91 is driven by centrifugal force. The outer sleeve 91 is inserted into the gas channel 52 along its length. A vent hole 911 is provided on the side wall of the outer sleeve 91 until the vent hole 911 reaches a position that corresponds to and communicates with the outlet hole 51. At this point, the outer sleeve 91 is fixed within the gas channel 52. The outer sleeve 91 can be made of wear-resistant copper tubing. The sealing body 93 is cylindrical. One end of the sealing body 93 within the moving area of ​​the outer sleeve 91 is the initial position, and the other end is the working position. When the sealing body 93 is in the initial position, it blocks the vent hole 911. When the sealing body 93 is in the working position, it is offset from the position of the vent hole 911. The reset element 92 is a tension spring. One end of the reset element 92 is fixed to the sealing body 93, and the other end is fixed to the outer sleeve 91. When the centrifugal force on the sealing body 93 is greater than the elastic force of the reset element 92, the sealing body 93 moves towards one end of the outer sleeve 91, gradually opening the vent hole 911 and connecting the interior of the gas channel 52 with the vent hole 51. When the rotational speed of the stirring blade 5 decreases, the elastic force of the reset element 92 is greater than the centrifugal force on the sealing body 93, thus pulling the sealing body 93 back to the position that just blocks the vent hole 911, sealing the vent hole 51 and preventing plasma from entering the gas channel 52.

[0052] A magnet 94 is also provided at one end of the outer tube 91. The magnet 94 has magnetic force, and the sealing body 93 is made of a material that can be attracted by the magnet 94, such as iron. The magnet 94 is located on the outer tube 91 at the end away from the working position of the sealing body 93, so that the magnet 94 and the reset member 92 can generate forces simultaneously when the sealing body 93 is in the initial position, so that the sealing body 93 can form a hysteresis effect and prevent the sealing body 93 from frequently opening or closing near the critical speed. In use, the attraction of the magnet 94 can open the sealing body 93 at a sufficient speed, and the sealing body 93 will only close when the speed drops below the opening speed.

[0053] The working process of this embodiment is as follows: First, during the process of gradually adding acid to the reaction tank 1, the stirring motor 11 starts to drive the drive shaft 12 to rotate. When the drive shaft 12 rotates, the active bevel gear 41 will rotate with the drive shaft 12. The active bevel gear 41 drives the driven bevel gear 43 to rotate through the connecting bevel gear 42. The driven bevel gear 43 is fixed on the stirring rod 2, so the stirring rod 2 will rotate in one direction under the action of the drive shaft 12, so that the stirring blade 5 installed on the stirring rod 2 will stir the plasma in the water-facing direction. At this time, the stirring rod 2 rotates.

[0054] While the drive shaft 12 rotates, the reciprocating screw 61, which is coaxial with the drive shaft 12 and connected by a key, also rotates synchronously. Since the slider 62, which cooperates with the reciprocating screw 61, is restricted from rotating by the anti-rotation frame 7, it will not rotate. At this time, the rotation of the reciprocating screw 61 will drive the slider 62 to slide back and forth in the vertical direction. The outer protrusion 621 of the slider 62 can move along the vertical direction. The protrusion 621 cooperates with the drive groove 311 to push the spiral drive groove 311, thereby causing the first connecting part 31 to rotate around the drive shaft 12. When the slider 62 moves back and forth once, the first connecting part 31 completes one swing. At this time, the stirring rod 2 will swing continuously under the action of the mounting frame 3.

[0055] When the speed of the stirring rod 2 needs to be increased, the sealing assembly 9 inside the stirring blade 5 begins to operate. When the centrifugal force on the sealing body 93 in the sealing assembly 9 exceeds the combined force of the reset member 92 and the magnet 94, the sealing body 93 moves along the outer sleeve 91 and reaches the working position, causing the sealing body 93 to open the vent hole 911. At this time, the vent hole 51 connects to the upper space of the reaction vessel 1 through the vent hole 911, the gas channel 52, and the hollow inside the stirring rod 2, allowing gas to enter the backwater surface of the stirring blade 5. Under the action of the low-pressure zone formed on the backwater surface, the air bubbles flow out from the vent hole 51 to reduce the cavitation formed by the stirring blade 5. At the same time, the gas that enters can flow upward in the plasma, forming a gas lift, further improving the mixing efficiency. When the speed of the stirring rod 2 decreases, the pulling force of the reset member 92 pulls the sealing body 93 back to the initial position, causing the sealing body 93 to seal the vent hole 911, preventing the plasma from entering the gas channel 52 through the vent hole 51.

[0056] In other embodiments, the sealing body 93 can be controlled electromagnetically. In other embodiments, the stirring blades 5 can also be provided in two or more layers, with multiple blades in each layer. In other embodiments, the reciprocating screw 61 can be driven by an electric cylinder or a hydraulic cylinder to drive the slider 62 to move vertically reciprocally.

[0057] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention.

Claims

1. A separation device for extracting serum protein, comprising a reaction tank and a stirring rod arranged in the reaction tank, a stirring blade being mounted on the stirring rod and rotating with the stirring rod, and a stirring motor being fixedly arranged on the reaction tank, characterized in that, Also include: The drive shaft is vertically arranged in the reaction tank and deviates from the center of the reaction tank, and the stirring motor is used to drive the drive shaft to rotate; The mounting frame includes a first connecting part and a second connecting part, the first connecting part is sleeved on the drive shaft and is in rotating connection with the drive shaft, and the second connecting part is used for rotatingly mounting the stirring rod; the first connecting part and the second connecting part are arranged at intervals and are in relative fixed connection; The transmission assembly is installed between the drive shaft and the stirring rod, and the stirring rod is driven to rotate by the drive shaft; The driving assembly is used for driving the first connecting part to rotate, so that the stirring rod swings around the drive shaft; The first connecting part is in a cylindrical shape, and a spiral driving groove is formed in the inner wall of the first connecting part; the driving assembly includes a vertically reciprocating sliding block, the outer side wall of the sliding block is provided with a protrusion, and the protrusion is slidably fitted in the driving groove; The stirring rod is in a hollow structure, the upper end of the stirring rod is communicated with the top of the reaction tank, the backwater surface of the stirring blade is provided with an air outlet hole, the air outlet hole is located away from the stirring rod, the stirring blade is provided with a gas passage, the air outlet hole is communicated with the hollow part in the stirring rod through the gas passage, and the position of the air outlet hole is provided with a plugging assembly for opening or closing the air outlet hole; The plugging assembly includes an outer sleeve, a reset member and a sealing body, the outer sleeve is fixed in the gas passage, the outer wall of the outer sleeve is provided with an air hole for communicating with the air outlet hole, the sealing body is slidably fitted in the outer sleeve, and the movement area of the sealing body along the outer sleeve has an initial position and a working position at two ends; when the sealing body is in the initial position, the sealing body blocks the air hole; when the sealing body is in the working position, the sealing body opens the air hole; the reset member is used to control the sealing body to be in the initial position or the working position; The movement of the sealing body in the outer sleeve is driven by the centrifugal force; when the centrifugal force acting on the sealing body is greater than the elastic force of the reset member, the sealing body moves along the outer sleeve to the working position, opens the air hole, so that the air outlet hole is communicated with the upper space of the reaction tank through the air hole, the gas passage and the hollow part in the stirring rod, the gas enters the backwater surface of the stirring blade, the bubbles flow out of the air outlet hole under the action of the low pressure area formed on the backwater surface, and the bubbles formed by the stirring blade are reduced; at the same time, the entering gas moves upward in the plasma to form gas stripping, and the mixing efficiency is improved; when the speed of the stirring rod decreases, the pulling force of the reset member pulls the sealing body back to the initial position again, so that the sealing body blocks the air hole and prevents the plasma from entering the gas passage through the air outlet hole.

2. The separation device for extracting serum proteins according to claim 1, wherein The drive shaft is coaxially provided with a reciprocating screw, which rotates with the drive shaft, and the sliding block is vertically guided by a rotation prevention frame fixed on the reaction tank; the reciprocating screw is matched with the sliding block, and the one-way rotation of the reciprocating screw is used to drive the sliding block to vertically reciprocate.

3. The separation device for extracting serum proteins according to claim 2, wherein The ratio of the pitch of the reciprocating screw to the pitch of the driving groove is 1:200-500.

4. The separation device for extracting serum proteins according to claim 1, wherein The transmission assembly comprises a driving bevel gear, a driven bevel gear and two connecting bevel gears coaxially fixed, a first connecting part and a second connecting part are fixed by a hollow cross bar, the connecting bevel gears are rotationally arranged inside the hollow cross bar, the driving bevel gear is coaxially fixed on the driving shaft, the driven bevel gear is coaxially fixed on the stirring rod, one connecting bevel gear meshes with the driving bevel gear, and the other connecting bevel gear meshes with the driven bevel gear.

5. The separation device for extracting serum proteins according to claim 1, wherein The reset member is a tension spring, one end of the tension spring is fixed on the sealing body, the other end is fixed on the outer sleeve, one end of the outer sleeve away from the working position of the sealing body is fixedly provided with a magnet, the magnet is used for attracting the sealing body, and the reset member and the magnet jointly keep the sealing body in the initial position.

6. The separation device for extracting serum proteins according to claim 1, wherein The gas passage is arranged inside the stirring blade and parallel to the stirring blade, the gas passage is opened at the edge of the stirring blade away from the one end of the stirring rod, the outer sleeve is inserted into the gas passage from the opening of the gas passage and fixed at the position where the air inlet hole and the air outlet hole are communicated.

7. The separation device for extracting serum proteins according to claim 1, wherein The lower part of the stirring blade is provided with a stirring paddle fixed on the stirring rod, the stirring paddle is in the shape of a sheet and arranged along the radial direction of the stirring rod, and the surface of the stirring blade is obliquely arranged with the axis of the stirring rod.

Images