Serum water-soluble vitamin extraction equipment based on liquid chromatography-mass spectrometry technology

Abstract

The invention relates to the technical field of analysis and detection, in particular to serum water-soluble vitamin extraction equipment based on a liquid chromatography-mass spectrometry technology, which comprises a rack, a precipitation cavity arranged on the rack and an ultrasonic generator arranged in the precipitation cavity, the upper push plate and the lower push plate are used for pushing supernate and precipitation liquid in the precipitation cavity respectively; during use, the upper push plate and the lower push plate are driven by the driving part to horizontally move in the precipitation cavity, and the upper push plate and the lower push plate are driven by the respective corresponding magnetic columns to respectively move; meanwhile, the transmission part can also drive the concentration turntable to rotate intermittently, the supernatant in the precipitation cavity can be quantitatively placed into the concentration turntable through a liquid discharge pipe and a solid-phase extraction column, a temperature-controlled nitrogen supply condition is provided for the concentration turntable in the evaporation barrel, and the quantitative supernatant in the concentration turntable is slowly evaporated under nitrogen flow, so that the volatile solvent is promoted to be completely volatilized; and finally, high-concentration target vitamins are enriched on the concentration turntable, so that the detection accuracy is ensured.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of analytical detection, and in particular to a serum water-soluble vitamin extraction device based on liquid chromatography-mass spectrometry technology. BACKGROUND

[0002] Extracting water-soluble vitamins from a complex biological matrix such as serum requires selecting a suitable solvent and method. Due to the high complexity of the sample and the presence of a large number of interfering substances inside, if the interfering substances are not completely removed, they will interfere with the analysis accuracy of subsequent liquid chromatography-mass spectrometry (LC-MS). The prior art uses an internal standard method combined with vortex oscillation for composite extraction, that is, an internal standard solution is added to the pretreated serum sample, an extraction agent is added, and the protein and interfering substances are removed by high-speed centrifugation. The target vitamin is completely dissolved by an ultrasonic generator, and finally the vitamin is extracted by a suitable solvent in combination with a balanced SPE column.

[0003] For example, the Chinese patent with application number CN202111628441.2 discloses a method for simultaneously detecting multiple water-soluble vitamins in serum based on liquid chromatography-tandem mass spectrometry. The steps are as follows: after adding an appropriate amount of internal standard solution to the serum sample, adding extraction agent I, vortex oscillation to mix the sample uniformly, standing, adding extraction agent II to the sample, vortex oscillation, transferring all the sample to an ultrafiltration tube, high-speed centrifugation, removing the ultrafiltration tube, transferring the lower layer of the centrifuged extraction liquid to a sample plate, adding extraction agent III, vortex oscillation to mix uniformly, and then loading the sample into a liquid chromatography-tandem mass spectrometer for detection. This method can quickly and accurately detect 11 kinds of water-soluble vitamins in serum. However, after vortex oscillation, the sample is directly loaded into the liquid chromatography-tandem mass spectrometer, and the volatile solvent in the extraction agent is not completely volatilized. The residual solvent not only interferes with the detection accuracy, but also causes the target vitamin content in the sample to be too low. The detection sensitivity of the low-concentration target vitamin is poor, which affects the accuracy of the subsequent vitamin peak recognition mass spectrometry data. SUMMARY

[0004] Therefore, the present application aims to provide a serum water-soluble vitamin extraction device based on liquid chromatography-mass spectrometry technology to solve the technical problem that the volatile solvent in the extraction agent is not completely volatilized when the sample is loaded, and the residual solvent not only interferes with the detection accuracy, but also causes the target vitamin content in the sample to be too low.

[0005] To achieve the above purpose, the present application provides a serum water-soluble vitamin extraction device based on liquid chromatography-mass spectrometry technology, which comprises a rack, a sedimentation cavity arranged on the rack, and an ultrasonic generator installed in the sedimentation cavity. The device further comprises: A mounting rack is arranged on the frame, and the sedimentation cavity is arranged between the mounting rack and the frame, and magnetic columns are arranged on both sides of the mounting rack in a sliding manner; An upper pushing plate and a lower pushing plate are arranged for pushing the supernatant and the precipitate in the sedimentation cavity, respectively, and the upper pushing plate and the lower pushing plate are respectively attracted to the magnetic columns on both sides of the mounting rack; A driving part is arranged for driving the upper pushing plate and the lower pushing plate to move horizontally in the sedimentation cavity; An evaporation barrel is arranged on the side of the frame; A concentration turntable and solid-phase extraction columns are arranged in the evaporation barrel, and the solid-phase extraction columns are arranged on the side end of the concentration turntable in a ring-shaped and uniform manner; A transmission part is arranged for driving the concentration turntable to rotate intermittently, so that when the supernatant is discharged through a discharge pipe on one side of the upper part of the sedimentation cavity, the solid-phase extraction columns are matched with the discharge pipe through end valves.

[0006] Further, a liquid inlet pipe is arranged through the mounting rack at the upper end of the sedimentation cavity, and a discharge pipe is further arranged on one side of the lower part of the sedimentation cavity.

[0007] Further, the driving part comprises: A driving motor is arranged on one side of the frame; A rotating shaft is arranged on the output end of the driving motor in a limiting and sliding manner; A pneumatic cylinder is arranged on the other side of the frame, and the other end of the rotating shaft is rotatably arranged on the output end of the pneumatic cylinder; Two opposite notched gears are arranged on the rotating shaft through a connecting key, and the two notched gears are alternately matched with the connecting key, respectively; A rack is arranged on both sides of the mounting rack in a sliding manner, the rack is engaged with the notched gears, and the magnetic columns are arranged on one side of each of the two racks, respectively.

[0008] Further, the magnetic attraction heights of the two magnetic columns are adjustable.

[0009] Further, guide rods are further arranged on both sides of the mounting rack, the rack is arranged on the guide rods in a sliding manner, springs are arranged on the guide rods on both sides of the rack, one end of the spring abuts against the rack, and the other end of the spring abuts against the mounting rack.

[0010] Further, the transmission part comprises: An L-shaped turnover arm is connected to the rotating shaft through a connecting key, and the end of the turnover arm penetrates the top of the evaporation barrel; An extraction pipe is rotatably arranged on the evaporation barrel, and the lower end of the extraction pipe is coaxially connected to the concentration turntable; Interleaved in the end of the turnover arm toggle, two said toggle with solid phase extraction column, two said toggle between the gap is provided for solid phase extraction column through.

[0011] Further, the lower part of the extraction pipe is annularly provided with suction holes, and the upper part of the extraction pipe is provided with a suction pump.

[0012] Further, it further comprises an opening volatile cover slidingly arranged on the concentration turntable, and the volatile cover is lifted and lowered on the concentration turntable through the transmission part.

[0013] Further, the volatile cover is coaxially slidingly arranged on the extraction pipe, the upper end of the volatile cover is provided with an inverted L-shaped baffle, the end of the turnover arm is rotationally provided with a push-pull slide rod, and the push-pull slide rod is slidingly arranged in the baffle.

[0014] Further, the side end of the evaporation barrel is provided with a nitrogen supply pipe and a gas collection cover.

[0015] The beneficial effects of the present application are as follows: when the serum water-soluble vitamin extraction equipment based on liquid chromatography mass spectrometry technology is used, the upper push plate and the lower push plate are driven to move horizontally in the sedimentation cavity by the driving part, and the upper push plate and the lower push plate are driven to move respectively by the respective corresponding magnetic columns; at the same time, the transmission part also drives the concentration turntable to rotate intermittently, the supernatant in the sedimentation cavity is quantitatively placed into the concentration turntable through the liquid discharge pipe and the solid phase extraction column, nitrogen gas supply conditions for temperature control of the concentration turntable in the evaporation barrel are provided, the quantitative supernatant in the concentration turntable is slowly evaporated under the nitrogen gas flow, the evaporation of the volatile solvent is promoted, and finally the high-concentration target vitamin is enriched in the concentration turntable, so that the detection accuracy is ensured. BRIEF DESCRIPTION OF DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the present application or prior art, the following will briefly introduce the drawings needed to be used in the embodiments or prior art description. Obviously, the drawings in the following description are only a part of the present application, and other drawings can also be obtained by those skilled in the art without creative labor.

[0017] Figure 1 It is a structural schematic diagram of the present application; Figure 2 It is a state diagram of the installation of the sedimentation cavity between the rack and the mounting frame in the present application; Figure 3 It is an internal structure diagram of the sedimentation cavity in the present application; Figure 4 It is an assembly diagram of the driving part from one perspective in the present application; Figure 5 It is an assembly diagram of the driving part from another perspective in the present application; Figure 6 Assembling diagram of driving part and transmission part in the application; Figure 7 Assembling diagram of concentration rotating disc and volatilization cover in the application; Figure 8 Structure diagram of evaporation barrel in the application.

[0018] Marked as: 1, rack; 2, sedimentation cavity; 3, ultrasonic generator; 4, mounting bracket; 5, magnetic column; 6, upper push plate; 7, lower push plate; 8, evaporation barrel; 9, concentration rotating disc; 10, liquid discharge pipe; 11, liquid inlet pipe; 12, discharge pipe; 13, driving motor; 14, rotating shaft; 15, air cylinder; 16, connecting key; 17, notched gear; 18, rack; 19, guide rod; 20, spring; 21, overturning arm; 22, extraction pipe; 23, actuating piece; 24, suction hole; 25, suction pump; 26, volatilization cover; 27, baffle; 28, push-pull slide rod; 29, nitrogen supply pipe; 30, gas collection cover. DETAILED DESCRIPTION

[0019] In order to make the purpose, technical scheme and advantages of the present application more clear, the present application is further described in detail below in combination with specific embodiments.

[0020] It should be noted that, unless otherwise defined, the technical terms or scientific terms used in the present application should be understood as the general meaning understood by those skilled in the art to which the present application belongs. The terms "first", "second" and similar terms used in the present application do not represent any order, number or importance, but are only used to distinguish different components. The terms "include" or "contain" and similar terms mean that the elements or objects before the terms cover the elements or objects listed after the terms and their equivalents, and do not exclude other elements or objects. The terms "connect" or "connected" and similar terms are not limited to physical or mechanical connection, but can include electrical connection, whether direct or indirect. The terms "up", "down", "left", "right" and the like are only used to represent relative positional relationship, and when the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0021] The first embodiment of the present application proposes a serum water-soluble vitamin extraction equipment based on liquid chromatography-mass spectrometry technology, as shown in Figures 1-8 The equipment comprises a rack 1, a sedimentation cavity 2 arranged on the rack 1, and an ultrasonic generator arranged in the sedimentation cavity 2. The equipment further comprises: A mounting bracket 4 is arranged on the rack 1, and the sedimentation cavity 2 is arranged between the mounting bracket 4 and the rack 1. Magnetic columns 5 are arranged on both sides of the mounting bracket 4 in a sliding manner. The upper push plate 6 and the lower push plate 7 are used to push the supernatant and the precipitate in the sedimentation cavity 2 respectively, and the upper push plate 6 and the lower push plate 7 are respectively attracted by the magnetic columns 5 on both sides of the mounting frame 4; The driving part is used to drive the horizontal movement of the upper push plate 6 and the lower push plate 7 in the sedimentation cavity 2; The evaporation barrel 8 is arranged on the side of the rack 1; The concentration turntable 9 is arranged in the evaporation barrel 8, and the solid-phase extraction column 3 is arranged on the side end of the concentration turntable 9 in a ring shape; The transmission part is used to drive the intermittent rotation of the concentration turntable 9, so that when the liquid discharge pipe 10 on one side of the upper part of the sedimentation cavity 2 discharges the supernatant, the solid-phase extraction column 3 is matched with the liquid discharge pipe 10 through the end valve.

[0022] In the embodiment, when in use, the sample liquid in the sedimentation cavity 2 is subjected to ultrasonic oscillation by the ultrasonic generator to completely dissolve the target vitamin. After a certain period of time, the sample liquid is stratified in the sedimentation cavity 2, the upper layer is the supernatant containing the target vitamin, and the lower layer is the precipitate layer of the interference substance containing protein. The driving part drives the horizontal movement of the upper push plate 6 and the lower push plate 7 in the sedimentation cavity 2, and the upper push plate 6 and the lower push plate 7 are respectively moved by the corresponding magnetic columns 5; At the same time, the transmission part also drives the intermittent rotation of the concentration turntable 9. The supernatant in the sedimentation cavity 2 is quantitatively placed into the concentration turntable 9 through the liquid discharge pipe 10 and the solid-phase extraction column 3. The concentration turntable 9 in the evaporation barrel 8 is provided with a nitrogen gas supply condition for temperature control. The quantitative supernatant in the concentration turntable 9 is slowly evaporated under the nitrogen gas flow to promote the complete evaporation of the volatile solvent. The above operation is repeated on the supernatant supplemented multiple times to enrich the target vitamin with high concentration in the concentration turntable 9.

[0023] In the embodiment, as shown in Figure 2 , Figure 3 , the upper end of the sedimentation cavity 2 is provided with a liquid inlet pipe 11 penetrating through the mounting frame 4, and the lower part of the sedimentation cavity 2 is also provided with a discharge pipe 12. The liquid is automatically fed into the sedimentation cavity 2 through the liquid inlet pipe 11. After ultrasonic treatment, the precipitate layer of the interference substance containing protein in the lower layer is discharged through the discharge pipe 12 to avoid the interference substance containing protein in the lower layer from being separated again into the supernatant in the upper layer.

[0024] In the embodiment, as shown in Figure 4 , Figure 5 , the driving part includes: The driving motor 13 is arranged on one side of the rack 1; The rotating shaft 14 is arranged on the output end of the driving motor 13 in a sliding manner at one end; The air cylinder 15 is arranged on the other side of the rack 1, and the other end of the rotating shaft 14 is rotatably arranged on the output end of the air cylinder 15; Two opposite notched gears 17 are installed on the rotating shaft 14 through the connecting keys 16 arranged at intervals, and the two notched gears 17 are alternately matched with the connecting keys 16, respectively; The rack 18 is slidably arranged on both sides of the mounting frame 4, the rack 18 is engaged with the notched gears 17, and the magnetic columns 5 are arranged on one side of each of the two racks 18, respectively; During use, the rotating shaft 14 is first driven to protrude by the output end of the cylinder 15, so that the connecting keys 16 on the rotating shaft 14 correspond to one side of the notched gears 17, and the other side of the notched gears 17 is separated from the connecting keys 16. At this time, the output end of the driving motor 13 rotates to drive the rotating shaft 14 to rotate, which drives the corresponding notched gears 17 to rotate through the connecting keys 16, and the notched gears 17 on the other side do not rotate. The notched gears 17 on the rotating side drive the racks 18 below to slide in the mounting frame 4, and then the magnetic columns 5 at the lower end drive the mutually attracted upper push plates 6 to slide, the sliding of the upper push plates 6 pushes the supernatant on the upper part of the sedimentation cavity 2, and the supernatant is quantitatively discharged through the discharge pipe 10; Then, the rotating shaft 14 is pulled back by the output end of the cylinder 15, so that the connecting keys 16 on the rotating shaft 14 correspond to the other side of the notched gears 17 and are separated from the corresponding notched gears 17. The driving motor 13 is started again, and the output end thereof is reversely rotated to drive the lower push plates 7 to slide, and the sliding of the lower push plates 7 pushes the protein-containing interference substance sediment layer in the lower layer of the sedimentation cavity 2, and the interference substance sediment layer is discharged through the discharge pipe 12.

[0025] In the embodiment, as shown in Figure 5 , the magnetic attraction heights of the two magnetic columns 5 are adjustable. According to different doses and different target vitamin concentrations of the sample liquid in the sedimentation cavity 2, the magnetic attraction heights of the two magnetic columns 5 are adjusted in time, which has strong adaptability.

[0026] In the embodiment, as shown in Figure 4 , the two sides of the mounting frame 4 are also provided with guide rods 19, the racks 18 are slidably arranged on the guide rods 19, springs 20 are sleeved on the guide rods 19 on both sides of the racks 18, one end of the spring 20 abuts against the rack 18, and the other end of the spring 20 abuts against the mounting frame 4. Under the action of the spring force of the spring 20 itself, the rack 18 can be pushed to reset to the original position along the direction of the guide rod 19, so that the notched gears 17 that lose the action of the connecting keys 16 are also reset to the original position, and the movement of the rotating shaft 14 in the next time can make the connecting keys 16 smoothly match with the notched gears 17 again.

[0027] In the embodiment, as shown in Figure 6 , Figure 7 , the transmission part comprises: The L-shaped turnover arm 21 connected with the rotating shaft 14 through the connecting keys 16, and the end of the turnover arm 21 penetrates the top of the evaporation barrel 8; A suction pipe 22 is arranged on the evaporation barrel 8, and a lower end of the suction pipe 22 is coaxially connected to the concentration rotating disc 9. The toggle plates 23 are arranged at the ends of the turning arms 21, and two toggle plates 23 are matched with the solid phase extraction column 3, and a gap is arranged between the two toggle plates 23 for the solid phase extraction column 3 to pass through. During use, the rotation of the rotating shaft 14 drives the turning arms 21 to rotate through the connecting keys 16, and then the toggle plates 23 drive the solid phase extraction column 3 to rotate by a fixed angle, and the rotating shaft 14 is reset, and then the other toggle plate 23 arranged in a staggered manner positions the concentration rotating disc 9, so that the drain pipe 10 can accurately align the corresponding solid phase extraction column 3 after rotation, and after the valve is opened, the supernatant is quantitatively placed into the concentration rotating disc 9 through the solid phase extraction column 3.

[0028] In this embodiment, as shown in Figure 6 , Figure 7 , a suction hole 24 is arranged in the lower part of the suction pipe 22 in a ring shape, and a suction pump 25 is arranged in the upper part of the suction pipe 22, and the high-concentration sample liquid in the concentration rotating disc 9 is placed into the suction pipe 22 through the suction hole 24 and is discharged by the suction pump 25, so as to extract the high-concentration sample liquid in the concentration rotating disc 9.

[0029] In this embodiment, as shown in Figure 7 , an opening volatile cover 26 is arranged on the concentration rotating disc 9 in a sliding manner, the volatile cover 26 is lifted and lowered on the concentration rotating disc 9 through a transmission part, the opening of the volatile cover 26 is opened during the lifting process, and the nitrogen supply condition outside the concentration rotating disc 9 can promote the volatilization and discharge of the volatile solvent in the high-concentration sample liquid, and the opening of the volatile cover 26 is closed during the lowering process, so as to avoid the excessive nitrogen drying of the sample liquid to cause the high-temperature decomposition of the target vitamin.

[0030] In this embodiment, as shown in Figure 7 , the volatile cover 26 is coaxially arranged on the suction pipe 22 in a sliding manner, an inverted trapezoidal baffle 27 is arranged at the upper end of the volatile cover 26, a push-pull slide rod 28 is arranged at the end of the turning arm 21 in a rotating manner, and the push-pull slide rod 28 is arranged in the baffle 27 in a sliding manner, and during the upward and downward turning of the turning arm 21, the push-pull slide rod 28 slides in the baffle 27, and then drives the volatile cover 26 to perform corresponding lifting and lowering actions.

[0031] In this embodiment, as shown in Figure 8 , a nitrogen supply pipe 29 and a gas collecting cover 30 are arranged at the side end of the evaporation barrel 8, the volatile solvent is collected in the gas collecting cover 30 through the nitrogen gas flow supplied by the nitrogen supply pipe 29, and the collection of the solvent is completed, so as to avoid diffusion into the laboratory environment.

[0032] Those skilled in the art should understand that the above discussion of any embodiment is merely exemplary in nature and is not intended to imply that the present application is limited to these examples; the above embodiments or technical features among different embodiments can also be combined, steps can be implemented in any order, and there are many other changes of different aspects of the present application as described above, which are not provided in details for the sake of brevity.

[0033] The present application is intended to cover all such alternatives, modifications, and variations as come within the scope of the broadest possible interpretation of the appended claims. Accordingly, any and all such alternations, modifications, equivalents, improvements and the like are intended to be encompassed by the present application.

Claims

1. A serum water-soluble vitamin extraction device based on liquid chromatography mass spectrometry technology, comprising a rack (1), a sedimentation cavity (2) arranged on the rack (1), and an ultrasonic generator installed in the sedimentation cavity (2), characterized in that, The device further comprises: a mounting rack (4) arranged on the frame (1), the sedimentation cavity (2) being arranged between the mounting rack (4) and the frame (1), and magnetic columns (5) being arranged on both sides of the mounting rack (4) in a sliding manner; an upper pushing plate (6) and a lower pushing plate (7) for respectively pushing supernatant and precipitate in the sedimentation cavity (2), the upper pushing plate (6) and the lower pushing plate (7) being respectively attracted to the magnetic columns (5) on both sides of the mounting rack (4); a driving part for driving the upper pushing plate (6) and the lower pushing plate (7) to move horizontally in the sedimentation cavity (2); an evaporation barrel (8) arranged on the side of the frame (1); a concentration turntable (9) arranged in the evaporation barrel (8) and solid-phase extraction columns (3) arranged on the side ends of the concentration turntable (9) in a ring-shaped manner; a transmission part for driving the concentration turntable (9) to rotate intermittently, so that, when the liquid discharge pipe (10) on one side of the upper part of the sedimentation cavity (2) discharges supernatant, the solid-phase extraction columns (3) pass through the end valves and cooperate with the liquid discharge pipe (10).

2. The serum water-soluble vitamin extraction apparatus based on liquid chromatography mass spectrometry technology according to claim 1, characterized in that, The upper end of the sedimentation cavity (2) is provided with a liquid inlet pipe (11) penetrating through the mounting rack (4), and the lower part of the sedimentation cavity (2) is further provided with a discharge pipe (12).

3. The serum water-soluble vitamin extraction apparatus based on liquid chromatography mass spectrometry technology according to claim 1, characterized in that, The driving part comprises: a driving motor (13) arranged on one side of the frame (1); a rotating shaft (14) arranged on the output end of the driving motor (13) in a limiting and sliding manner; an air cylinder (15) arranged on the other side of the frame (1), the other end of the rotating shaft (14) being rotatably arranged on the output end of the air cylinder (15); two opposite notched gears (17) being arranged on the rotating shaft (14) through the connecting keys (16) arranged at intervals, and the two notched gears (17) being alternately matched with the connecting keys (16) respectively; a rack (18) arranged on both sides of the mounting rack (4) in a sliding manner, the rack (18) being engaged with the notched gears (17), and the magnetic columns (5) being arranged on one side of each of the two racks (18).

4. The serum water-soluble vitamin extraction apparatus based on liquid chromatography mass spectrometry technology according to claim 3, characterized in that, The magnetic attraction heights of the two magnetic columns (5) are adjustable.

5. The serum water-soluble vitamin extraction apparatus based on liquid chromatography mass spectrometry technology according to claim 3, characterized in that, The mounting rack (4) is further provided with guide rods (19), the rack (18) is arranged on the guide rods (19) in a sliding manner, springs (20) are arranged on the guide rods (19) on both sides of the rack (18), one end of the spring (20) abuts against the rack (18), and the other end of the spring (20) abuts against the mounting rack (4).

6. The serum water-soluble vitamin extraction apparatus based on liquid chromatography mass spectrometry technology according to claim 3, characterized in that, The transmission part comprises: an L-shaped turnover arm (21) connected with the rotating shaft (14) through the connecting keys (16), the end of the turnover arm (21) penetrating through the top of the evaporation barrel (8); an extraction pipe (22) rotatably arranged on the evaporation barrel (8), the lower end of the extraction pipe (22) being coaxially connected to the concentration turntable (9); dialing pieces (23) arranged on the end of the turnover arm (21) in a staggered manner, the two dialing pieces (23) being matched with the solid-phase extraction columns (3), and a gap for the solid-phase extraction columns (3) to pass through being arranged between the two dialing pieces (23).

7. The serum water-soluble vitamin extraction apparatus based on liquid chromatography mass spectrometry technology according to claim 6, characterized in that, The lower part of the extraction tube (22) is provided with a suction hole (24) in a circumferential direction, and the upper part of the extraction tube (22) is provided with a suction pump (25).

8. The serum water-soluble vitamin extraction apparatus based on liquid chromatography mass spectrometry technology according to claim 6, characterized in that; It also includes an open evaporation cap (26) that is slidably disposed on the concentration turntable (9), the evaporation cap (26) moving up and down on the concentration turntable (9) via the transmission unit.

9. The serum water-soluble vitamin extraction apparatus based on liquid chromatography mass spectrometry technology according to claim 8, characterized in that, The evaporation cap (26) is slidably mounted on the extraction tube (22) on the same axis. The upper end of the evaporation cap (26) is provided with an inverted baffle (27). The end of the flipping arm (21) is rotatably provided with a push-pull slide rod (28), which is slidably mounted inside the baffle (27).

10. The serum water-soluble vitamin extraction apparatus based on liquid chromatography mass spectrometry technology according to claim 1, characterized in that, The side end of the evaporation tank (8) is provided with a nitrogen supply pipe (29) and a gas collection hood (30).

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