Application of early-stage chick embryo amniotic fluid in preparation of anti-alcoholism medicine

Abstract

The invention relates to a new application of early-stage chick embryo amniotic fluid, which is characterized in that the early-stage chick embryo amniotic fluid is found to be capable of effectively dispelling the effects of alcohol, removing the content of ethanol in serum and increasing the levels of liver acetaldehyde dehydrogenase and ethanol dehydrogenase by evaluating the effect of dispelling the effects of alcohol of the early-stage chick embryo amniotic fluid, and also has the effects of protecting the gastric mucosa function and the like.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of medicine, and particularly relates to application of early chick embryo amniotic fluid in preparation of an alcoholism relieving medicine. BACKGROUND

[0002] According to the data of the World Health Organization, about 3.3 million people die every year due to excessive drinking, accounting for 6% of the global death toll. The medical resource burden and financial cost for alcohol caused by excessive drinking seriously threaten the physical and mental health of people all over the world, especially in China, and become a serious burden on social development. Long-term heavy drinking leads to intestinal flora imbalance, and intestinal epithelial barrier dysfunction caused thereby causes endotoxin into the blood, resulting in liver damage; ethanol can produce reactive oxygen species to damage the liver in the process of oxidation, secondly, the produced acetaldehyde can directly damage hepatocytes; in addition, a large amount of oxygen is consumed in the metabolism of alcohol in the body, which can cause hypoxia in the liver lobule. High concentration of alcohol can also cause contraction of liver blood vessels, resulting in reduced blood flow in the liver, insufficient oxygen supply, and triggering of liver microcirculation disorder. At present, the treatment of patients with excessive drinking in the clinic mainly focuses on removing residual alcohol and correcting metabolic disorders, and then giving nutritional support and drug treatment, especially drugs for relieving and protecting the stomach function. Therefore, how to effectively research the treatment drugs for alcoholism, timely remove the ethanol content in the human body, and prevent excessive drinking and alcoholism is still the focus of current research.

[0003] The research group has carried out application research on early chick embryo amniotic fluid as a natural source of medicine in many aspects, such as radioactive organ damage and colitis. This provides a certain theoretical basis for exploring the alcoholism relieving function of early chick embryo amniotic fluid. SUMMARY

[0004] The application provides application of early chick embryo amniotic fluid in preparation of an alcoholism relieving product, which has the effects of removing ethanol in blood, increasing ethanol dehydrogenase and acetaldehyde dehydrogenase, and protecting gastric mucosa.

[0005] The specific technical scheme of the application is as follows:

[0006] The application of amniotic fluid in preparation of an alcoholism relieving product, wherein the amniotic fluid is obtained from an early chick embryo or an early embryo of other birds except for chickens.

[0007] Preferably, the early chick embryo is a 5-9 day old chick embryo.

[0008] More preferably, the early chick embryo is a 6-8 day old chick embryo.

[0009] In some embodiments, the alcoholism relieving product is a medicine.

[0010] Preferably, the dosage form of the medicine is tablet, capsule, oral liquid, granule, suspension, syrup or injection.

[0011] In some embodiments, the hangover product is a health product.

[0012] Preferably, the form of the health product is tablet, capsule, oral liquid, syrup, granule, gummy or pressed candy.

[0013] Compared with the prior art, the present application has the following advantages:

[0014] 1. By evaluating the efficacy of early chicken embryo amniotic fluid in resolving alcohol, it is found through animal experiments that early chicken embryo amniotic fluid can remove the ethanol content in serum, increase the levels of acetaldehyde dehydrogenase and ethanol dehydrogenase in liver, and protect the function of gastric mucosa, thereby playing a synergistic role in resolving alcohol from multiple aspects.

[0015] 2. The medical and health care effects of early chicken embryo amniotic fluid are further developed.

[0016] 3. Compared with other hangover products, early chicken embryo amniotic fluid is inexpensive and easy to obtain, simple to prepare, and can be produced on an industrial scale. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 A is the analysis result of the effect of early chicken embryo amniotic fluid treatment on the ethanol level in serum of mice with acute excessive alcohol consumption (*P<0.05, **P<0.01, ***P<0.001).

[0018] Figure 1 B is the analysis result of the effect of early chicken embryo amniotic fluid treatment on the ethanol dehydrogenase level in liver of mice with acute excessive alcohol consumption (*P<0.05, **P<0.01, ***P<0.001).

[0019] Figure 1 C is the analysis result of the effect of early chicken embryo amniotic fluid treatment on the acetaldehyde dehydrogenase level in liver of mice with acute excessive alcohol consumption (*P<0.05, **P<0.01, ***P<0.001).

[0020] Figure 1 D is the analysis result of the effect of early chicken embryo amniotic fluid treatment on the calcium ion level in gastric tissue of mice with acute excessive alcohol consumption (*P<0.05, **P<0.01, ***P<0.001).

[0021] Figure 1 E is the analysis result of the effect of early chicken embryo amniotic fluid treatment on the myeloperoxidase level in gastric tissue of mice with acute excessive alcohol consumption (*P<0.05, **P<0.01, ***P<0.001). DETAILED DESCRIPTION

[0022] The following examples are intended to enable a person skilled in the art to more fully understand the technical solutions and implementation effects of the present application, but the protection scope of the present application is not limited thereto. This part will make a further detailed description of the present application combined with specific implementation cases. The technical features and advantages will be clearly embodied in the description. It should be pointed out that the examples are only illustrative and do not limit the scope of the claims of the present application. Any detail adjustment, equivalent replacement or adaptive improvement based on the core principle of the present application all belong to the essential protection scope of the present application.

[0023] Example 1: Study on the alcoholism treatment effect of early chick embryo amniotic fluid

[0024] (I) Experimental animals

[0025] In this implementation case, 24 C57BL / 6 male mice aged 8 weeks were purchased from Jiangsu Suzhou Sbiopharm Biological Technology Co., Ltd. They were bred in the SPF level experimental animal center of Wuxi Medical College of Jiangnan University, and the experimental scheme was approved by the Animal Experiment Ethics Committee of Wuxi Medical College of Jiangnan University. All experimental animals were bred in an environment with a temperature of 22-26℃, a relative humidity of 40-70%, and a 12h light / 12h dark cycle, and the animals could freely eat and drink water.

[0026] (II) Preparation of early chick embryo amniotic fluid

[0027] Fertilized eggs were incubated at 37±1℃ and 50% humidity. Sterile syringes were used to collect the amniotic fluid of chicken embryos incubated for 6-8 days. The collected amniotic fluid was centrifuged at 12,000r / min for 30min and stored at -80℃ for a long time.

[0028] (III) Construction of acute excessive alcohol consumption mouse model and intervention of early chick embryo amniotic fluid

[0029] The 24 C57BL / 6 male mice were randomly divided into 4 groups according to their body weight, with 6 mice in each group, namely: control group (fasting without water restriction, one-time gavage of 10ml / kg pure water, one hour later, eyeball blood sampling and then sacrifice); acute alcohol group (fasting without water restriction, one-time gavage of 10ml / kg absolute ethanol, one hour later, eyeball blood sampling and then sacrifice); early chick embryo amniotic fluid low concentration group (fasting without water restriction, feeding 7.5ml / kg early chick embryo amniotic fluid (L-ceAF group) for 30min, one-time gavage of 10ml / kg absolute ethanol, one hour later, eyeball blood sampling and then sacrifice); early chick embryo amniotic fluid high concentration group (fasting without water restriction, feeding 15ml / kg early chick embryo amniotic fluid (H-ceAF group) for 30min, one-time gavage of 10ml / kg absolute ethanol, one hour later, eyeball blood sampling and then sacrifice).

[0030] (IV) Experimental results

[0031] 1. Early chick embryo amniotic fluid reduces blood ethanol concentration in acute excessive alcohol consumption mice

[0032] In this embodiment, the effect of early chick embryo amniotic fluid on alcohol metabolism was evaluated by detecting the blood ethanol concentration in mice. As shown in Figure 1 As shown in A, compared with the control group mice (control group), the ethanol concentration in the serum of acute excessive alcohol consumption mice (AGC group) was significantly increased (p < 0.001), and compared with the acute excessive alcohol consumption mice (AGC group), high concentration of early chick embryo amniotic fluid could significantly reduce the ethanol content in mice (p < 0.001), and low concentration of early chick embryo amniotic fluid could significantly reduce the ethanol content in mice (p < 0.01), indicating that early chick embryo amniotic fluid could reduce the ethanol concentration in acute alcohol consumption mice and had good alcohol metabolism effect.

[0033] 2. Effect of early chick embryo amniotic fluid on alcohol metabolism related enzymes in acute excessive alcohol consumption mice

[0034] Alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) are key enzymes in the classic pathway of alcohol metabolism in vivo. In this embodiment, the effect of early chick embryo amniotic fluid on alcohol metabolism was further evaluated by detecting the content of ADH and ALDH. As shown in Figure 1 As shown in B and 1C, compared with the control group mice (control group), the content of ADH in the liver tissue of acute excessive alcohol consumption mice (AGC group) was significantly decreased (p < 0.001), and the content of ALDH was significantly decreased (p < 0.01), and compared with the acute excessive alcohol consumption group (AGC group), high concentration of early chick embryo amniotic fluid significantly increased the content of ADH in the liver tissue of mice (p < 0.01) and significantly increased the content of ALDH in the liver tissue of mice (p < 0.05). The above results showed that early chick embryo amniotic fluid could activate the activity of ADH and ALDH to some extent, accelerate alcohol metabolism, thereby reduce the blood ethanol content and achieve the effect of alcohol metabolism.

[0035] 3. Effect of early chick embryo amniotic fluid on gastric mucosal barrier in acute excessive alcohol consumption mice

[0036] The effect of early chick embryo amniotic fluid on gastric mucosal barrier in acute excessive alcohol consumption mice was studied by detecting the calcium ion content and myeloperoxidase (MPO) activity of gastric tissue. The results are shown in Figure 1Compared with the control group, the calcium ion content in the stomach tissue of the acute excessive alcohol consumption mice increased significantly (p<0.001), and the MPO activity increased significantly (p<0.05). Compared with the acute excessive alcohol consumption mice group, the calcium ion content in the stomach tissue of the mice in the different concentrations of early chicken embryo amniotic fluid groups decreased significantly (p<0.001), and the MPO content in the high concentration of early chicken embryo amniotic fluid group decreased significantly (p<0.05). The above results show that alcohol leads to a large number of inflammatory cells invading the submucosal tissue of the mouse stomach, and early chicken embryo amniotic fluid can alleviate the damage to the gastric mucosal barrier caused by alcohol and protect the gastric mucosa.

[0037] Finally, it should be noted that the above examples are only used to illustrate and not to limit the technical solutions of the present application. Although the present application has been described in detail with reference to the above examples, those skilled in the art should understand that the present application can still be modified or replaced by equivalents without departing from the spirit and scope of the present application. Any modification or partial replacement should be covered in the scope of the claims of the present application.

Claims

1. Use of amniotic fluid in the preparation of an anti-alcohol product, characterized in that, The amniotic fluid is obtained from an early chicken embryo or an early embryo of another avian species other than chicken.

2. Use according to claim 1, characterized in that, The early chicken embryo is a 5-9 day old chicken embryo.

3. Use according to claim 2, characterised in that, The early chicken embryo is a 6-8 day old chicken embryo.

4. Use according to any one of claims 1 to 3, characterized in that, The hangover product is a drug.

5. Use according to claim 4, characterized in that, The dosage form of the drug is a tablet, a capsule, an oral liquid, a granule, a suspension, a syrup or an injection.

6. Use according to any one of claims 1 to 3, characterized in that, The hangover product is a health product.

7. Use according to claim 6, characterized in that, The form of the health product is a tablet, a capsule, an oral liquid, a syrup, a granule, a soft candy or a pressed candy.

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