System and method for monitoring state of front light system

Abstract

The invention discloses a state monitoring system of a front light system. The state monitoring system comprises a reflector, a front light detector and a controller, the reflector is located in the front light system, faces the laser, forms a preset angle with the direction of the light signal emitted by the laser, and is used for reflecting the light signal emitted by the laser to the front light detector; the front light detector is connected with the controller and is used for converting the light signal reflected by the reflector into the light power of the laser and transmitting the light power to the controller; and the controller is used for determining a state monitoring result of the front light system based on a relationship between the light power and a preset light power. Thus, the optical power corresponding to the laser can be collected based on the reflector and the front light detector, then the state of the front light system is monitored based on the optical power, and the monitoring efficiency is improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of detection, in particular to a state monitoring system and method of a front light system. BACKGROUND

[0002] The stability and repeatability of flow cytometry, especially spectral flow cytometry, are increasingly valued, because spectral library is involved, and the state of the front light system is very crucial for the measurement of the instrument. Therefore, it is of great significance to monitor the state of the front light system. SUMMARY

[0003] Therefore, the embodiments of the present application at least provide a state monitoring system and method of a front light system.

[0004] The technical scheme of the embodiments of the present application is implemented as follows:

[0005] The embodiments of the present application provide a state monitoring system of a front light system, which comprises a reflector, a front light detector, and a controller.

[0006] The reflector is located in the front light system, faces the laser and is at a preset angle with the direction of the light signal emitted by the laser, and is used to reflect the light signal emitted by the laser in the front light system to the front light detector after passing through the flow chamber.

[0007] The front light detector is connected with the controller, is used to convert the light signal reflected by the reflector into the optical power of the laser, and transmit the optical power to the controller.

[0008] The controller is used to determine the state monitoring result of the front light system based on the relationship between the optical power and the preset optical power.

[0009] In the above state monitoring system of the front light system, the reflector is arranged on the side of the forward scattering detector facing the laser in the front light system, is at a preset angle with the forward scattering detector, and is used to reflect the light signal emitted by the laser to the front light detector after passing through the flow chamber.

[0010] In the above state monitoring system of the front light system, the laser in the front light system comprises at least one first laser; the front light detector is used to determine the corresponding first optical power when each first laser in the at least one first laser works alone, and obtain at least one first optical power.

[0011] The controller is used to compare each first optical power in the at least one first optical power with the preset optical power, obtain at least one comparison result, and determine the state information of each first laser and / or the state information of the flow chamber in the front light system based on the at least one comparison result.

[0012] The reflector comprises at least one mirror set corresponding to the at least one first laser;

[0013] Each of the at least one mirror set is arranged between a corresponding first laser and the flow chamber of the at least one first laser, and is configured to reflect the light signal emitted by the corresponding first laser to the front light detector.

[0014] The front light detector comprises a current-to-voltage circuit, a filter circuit, and an analog-to-digital converter.

[0015] The current-to-voltage circuit is connected to the filter circuit, and is configured to convert the light signal reflected by the reflector into a voltage signal and input the converted voltage signal to the filter circuit.

[0016] The filter circuit is connected to the analog-to-digital converter, and is configured to filter the converted voltage signal to obtain a filtered voltage signal.

[0017] The analog-to-digital converter is connected to the filter circuit, and is configured to convert the filtered voltage signal into the optical power of the laser.

[0018] The front light detector further comprises a buffer circuit.

[0019] The buffer circuit is arranged between the current-to-voltage circuit and the filter circuit, and is configured to reduce the voltage drop between the output resistance of the current-to-voltage circuit and the input resistance of the filter circuit, so as to input the converted voltage signal to the filter circuit.

[0020] The front light detector further comprises a voltage amplification circuit.

[0021] The voltage amplification circuit is arranged between the current-to-voltage circuit and the filter circuit, and is configured to perform secondary amplification on the converted voltage signal output by the current-to-voltage circuit in response to the amplification factor of the current-to-voltage circuit being less than a preset amplification factor.

[0022] The current-to-voltage circuit comprises a first operational amplifier and a first resistor.

[0023] The first resistor is connected in parallel to the inverting input terminal and the output terminal of the first operational amplifier, and is configured to adjust the amplification factor of the current-to-voltage circuit to the preset amplification factor.

[0024] The first operational amplifier has a ground connected to the non-inverting input terminal and an output terminal connected to the buffer circuit.

[0025] The filter circuit comprises at least one of the following:

[0026] a filter circuit, disposed between the current-to-voltage circuit and the voltage amplification circuit or the buffer circuit, for filtering the converted voltage signal;

[0027] a filter circuit, disposed between the voltage amplification circuit or the buffer circuit and the analog-to-digital converter, for filtering the preset voltage signal;

[0028] a filter circuit, connected to the analog-to-digital converter, for filtering the voltage signal input to the analog-to-digital converter.

[0029] In the above state monitoring system of the front light system, the front light system monitored by the state monitoring system is a system for providing a light signal in a flow cytometer.

[0030] Embodiments of the present application provide a state monitoring method of a front light system, applied to a state monitoring system of the front light system, the state monitoring system comprising a reflector, a front light detector, and a controller; the reflector is located in the front light system, facing a laser and at a preset angle to a direction of light signals emitted by the laser; the front light detector is connected to the controller; the method comprises:

[0031] the reflector reflects the light signals emitted by the laser to the front light detector;

[0032] the front light detector converts the light signals reflected by the reflector into optical power of the laser, and transmits the optical power to the controller;

[0033] the controller determines a state monitoring result of the front light system based on a relationship between the optical power and a preset optical power.

[0034] In the above state monitoring method of the front light system, the laser in the front light system comprises at least one first laser; the method further comprises: the front light detector determines a corresponding first optical power when each of the at least one first laser works alone, to obtain at least one first optical power;

[0035] the controller compares each of the at least one first optical power with the preset optical power to obtain at least one comparison result, and determines state information of each of the at least one first laser and / or state information of the flow chamber based on the at least one comparison result.

[0036] It should be understood that the above general description and the following detailed description are only exemplary and explanatory, but not limiting the technical solutions of the present application. BRIEF DESCRIPTION OF DRAWINGS

[0037] The accompanying drawings, which are incorporated into and form part of the specification, illustrate embodiments consistent with the present application and, together with the specification, serve to explain the technical solutions of the present application.

[0038] Figure 1 is a structural schematic diagram of an exemplary instrument containing a front light system provided by the related art;

[0039] Figure 2 is a structural schematic diagram of an exemplary state monitoring system for a front light system provided by the present application Figure 1 ;

[0040] Figure 3 is a structural schematic diagram of an exemplary state monitoring system for a front light system provided by the present application Figure 2 ;

[0041] Figure 4 is a structural schematic diagram of an exemplary state monitoring system for a front light system provided by the present application Figure 3 ;

[0042] Figure 5 is a structural schematic diagram of an exemplary state monitoring system for a front light system provided by the present application Figure 4 ;

[0043] Figure 6 is a structural schematic diagram of an exemplary state monitoring system for a front light system provided by the present application Figure 5 ;

[0044] Figure 7 is a structural schematic diagram of an exemplary front light detector provided by the present application Figure 1 ;

[0045] Figure 8 is a structural schematic diagram of an exemplary front light detector provided by the present application Figure 2 ;

[0046] Figure 9 is a structural schematic diagram of an exemplary front light detector provided by the present application Figure 3 ;

[0047] Figure 10 is a structural schematic diagram of an exemplary front light detector provided by the present application Figure 4 ;

[0048] Figure 11 is a structural schematic diagram of an exemplary front light detector provided by the present application Figure 5 ;

[0049] Figure 12 is a structural schematic diagram of an exemplary front light detector provided by the present application Figure 6 ;

[0050] Figure 13is a structural schematic of an example front light detector provided by an embodiment of the present application Figure 7 ;

[0051] Figure 14 is a structural schematic of an example front light detector provided by an embodiment of the present application Figure 8 ;

[0052] Figure 15 is a structural schematic of an example front light detector provided by an embodiment of the present application Figure 9 ;

[0053] Figure 16 is a flow schematic of an example state monitoring method provided by an embodiment of the present application Figure 1 ;

[0054] Figure 17 is a flow schematic of an example state monitoring method provided by an embodiment of the present application Figure 2 . DETAILED DESCRIPTION

[0055] In order to make the purposes, technical solutions and advantages of the present application clearer, the technical solutions of the present application are further described in detail below in combination with the drawings and embodiments, and the described embodiments should not be regarded as limiting the present application, and all other embodiments obtained by those skilled in the art without making creative efforts fall within the scope of protection of the present application.

[0056] In the following description, "some embodiments" are related to a subset of all possible embodiments, but it can be understood that "some embodiments" can be the same subset or different subsets of all possible embodiments, and can be combined with each other without conflict.

[0057] The term "first / second / third" involved only distinguishes similar objects, and does not represent a specific order of the objects, and it can be understood that "first / second / third" can interchange the specific order or sequence as allowed, so that the embodiments of the present application described herein can be implemented in an order other than that illustrated or described herein.

[0058] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the present application belongs. The terms used herein are only for the purpose of describing the present application, and are not intended to limit the present application.

[0059] As Figure 1As shown, a structural schematic diagram of an example front light system 10 of a flow cytometer (instrument) 1 is provided, the front light system 10 including a laser 11, a flow chamber 12, a forward scattering detector 13, and a lens 14. When the light signal emitted by the laser 11 passes through the flow chamber 12, forward scattering and side scattering occur, and then the forward scattering light is input into a receiver of the forward scattering detector 13, and the side scattering passes through the lens 14 to reach a rear light system 15.

[0060] In the related art, the front light power is basically not concerned. However, the front light system light intensity detection can determine the state of the flow chamber, whether there is pollution, or whether the laser installation is normal, whether the optical power is normal, and the like, and has important significance.

[0061] An embodiment of the present application provides a front light system state monitoring system, Figure 2 A structural schematic diagram of a front light system state monitoring system 20 provided by an embodiment of the present application is shown.

[0062] As shown, Figure 3 The state monitoring system 20 includes a reflector 21, a front light detector 22, and a controller 23. The reflector 21 is located in the front light system 10, faces the laser 11, and is at a preset angle with the laser 11, for reflecting the light signal emitted by the laser 11 to the front light detector 22. The front light detector 22 is connected with the controller 23, for converting the light signal reflected by the reflector 21 into the optical power of the laser 11, and transmitting the optical power to the controller 23. The controller 23 is used for determining the state monitoring result of the front light system 10 based on the relationship between the optical power and a preset optical power.

[0063] In an embodiment of the present application, the state monitoring system includes a reflector 21, which is located in the front light system 10, faces the laser 11, and is at a preset angle with the direction of the light signal emitted by the laser 11. For example, the preset angle can be 45°, 15°, or other degrees. The size of the preset angle affects the light signal reflected to the front light detector 22, which can be set based on the required light signal of the front light detector 22. For example, the preset angle can be set based on actual needs and application scenarios, which is not limited in the present application.

[0064] In an embodiment of the present application, the reflector 21 can be a plane mirror, a curved mirror, a mirror group, a turning mirror, a total reflector, or a combination of multiple reflectors. The selection of the reflector 21 and the combination of multiple reflectors can be set based on actual needs and application scenarios, which is not limited in the present application.

[0065] In an embodiment of the present application, the front light detector 22 is connected with the controller 23, can convert the light signal reflected by the reflector 21 into the optical power, and then transmit the optical power to the controller 23.

[0066] In the embodiment of the present application, the preset optical power is a standard optical power calibrated in advance. For example, when the instrument (including the front light system 10) is shipped, the background (light, electricity) and the optical power of the laser 11 after passing through the flow chamber 12 are monitored. The optical power measured when the laser 11 is turned off is the background optical power. When the laser 11 is turned on, the measured optical power minus the background optical power is the standard optical power corresponding to the laser 11, i.e. the preset optical power.

[0067] In the embodiment of the present application, the preset optical power can also be a certain tolerance set based on the standard optical power. For example, the preset optical power is set with a tolerance of 5% up and down based on the standard optical power. In this way, a certain error of the laser 11 is allowed, but once the error exceeds the set error, the accuracy of the front light system 10 can be low. In order to require higher accuracy, the up and down tolerance can be set to 3% or 1%, etc. For example, the up and down tolerance can be set based on actual needs and application scenarios, which is not limited in the present application.

[0068] In the embodiment of the present application, if the preset angle between the reflector 21 and the direction of the light signal emitted by the laser 11 is 15° when tested before shipment, the preset angle between the reflector 21 and the direction of the light signal emitted by the laser 11 is still 15° after shipment. In this way, the optical power obtained before and after shipment is consistent, which has comparability.

[0069] In the embodiment of the present application, in order to ensure the accuracy of the measurement, the background optical power can be measured again when the optical power is detected after shipment. Of course, the measurement can also be based on the previous background optical power.

[0070] In the embodiment of the present application, after the front light detector 22 transmits the optical power to the controller 23, the state monitoring result of the front light system 10 can be determined based on the relationship between the optical power and the preset optical power. For example, if the optical power is less than the preset optical power, it means that the laser 11 is attenuated. If the optical power is greater than the preset optical power, it means that the laser 11 is abnormal.

[0071] In the embodiment of the present application, the self-checking of the front light system 10 can be performed after the instrument starts to boot, or after a preset time period. For example, the self-checking can be performed after booting, or based on the service life of the laser 11 or based on experience that the attenuation will occur after a certain period of use, etc. For example, the self-checking can be performed after three months, two weeks, one month, or one week, etc. For example, the timing of the self-checking can be set based on actual needs and application scenarios, which is not limited in the present application.

[0072] Thus, the light signal reflected by the laser 11 is reflected to the front light detector 22 by the reflector 21 at a preset angle with the laser 11, and then the state of the front light system 10 is monitored based on the light power corresponding to the light signal to obtain a state monitoring result, so that real-time monitoring is achieved and the troubleshooting efficiency is improved.

[0073] As shown in Figure 4 The reflector 21 is arranged on the side of the forward scattering detector 13 in the front light system 10 facing the laser 11 at a preset angle with the forward scattering detector 13, and is used to reflect the light signal emitted by the laser 11 to the front light detector 22 after passing through the flow chamber 12.

[0074] Exemplarily, the reflector 21 is arranged on the side of the forward scattering detector 13 in the front light system 10 facing the laser 11 at a preset angle with the forward scattering detector 13, and is used to reflect the light signal emitted by the laser 11 to the front light detector 22 after passing through the flow chamber 12. The front light detector 22 is connected with the controller 23, and is used to convert the light signal reflected by the reflector 21 into the light power of the laser 11, and transmit the light power to the controller 23. The controller 23 is used to determine the state monitoring result of the front light system 10 based on the relationship between the light power and the preset light power.

[0075] In the embodiment of the present application, the state monitoring system includes a reflector 21 arranged at the receiver of the forward scattering detector 13 in the front light system 10 at a first preset angle with the receiver. The light signal emitted by the laser 11 will be incident into the receiver of the forward scattering detector 13 after passing through the flow chamber 12, and the reflector 21 can reflect part of the forward scattering light incident into the forward scattering detector 13 to the front light detector 22.

[0076] In the embodiment of the present application, if the preset angle between the reflector 21 and the forward scattering detector 13 is 25° when tested before leaving the factory, then the preset angle between the reflector 21 and the forward scattering detector 13 is still 25° after leaving the factory. In this way, the light powers obtained before and after leaving the factory are comparable.

[0077] In the embodiment of the present application, the light signal received by the front light detector 22 is the light signal reflected after passing through the flow chamber 12 by the laser 11. Therefore, based on the relationship between the light power and the preset light power, the state monitoring result of the front light system 10 can be that if the light power is less than the preset light power, it indicates that the laser 11 is attenuated or the flow chamber 12 is abnormal. The abnormality of the flow chamber 12 can include the abnormality of the inner wall or the outer wall of the flow chamber 12. If the light power is greater than the preset light power, it indicates that the laser 11 is abnormal.

[0078] Thus, the light signal reflected by the laser 11 through the flow chamber 12 is reflected to the front light detector 22 by the reflector 21 at a preset angle with the forward scattering detector 13, and then the state of the front light system 10 is monitored based on the light power corresponding to the light signal to obtain a state monitoring result, so that real-time monitoring can be achieved and the troubleshooting efficiency is improved.

[0079] In some embodiments, the front light system 10 monitored by the state monitoring system 20 is a system arranged in a flow cytometer to provide a light signal.

[0080] In the embodiments of the present application, the front light system 10 can be arranged in a flow cytometer (instrument) 1, or can be arranged in other instruments, such as a laser scanning microscope (confocal microscope), a spectrum analyzer, a laser dust particle counter, or other instruments requiring front light.

[0081] In the embodiments of the present application, the front light system 10 in the instrument 1 including the laser 11, the flow chamber 12, the forward scattering detector 13, and the lens 14 can all use the state monitoring system provided in the embodiments of the present application.

[0082] In some embodiments, as shown in FIG. 1, the front light system 10 includes at least one first laser 111, a front light detector 22, and a controller 23. Figure 5 The front light detector 22 is configured to determine a corresponding first light power when each of the at least one first laser 111 works alone, to obtain at least one first light power.

[0083] In the embodiments of the present application, the front light system 10 can include at least one first laser 111, and for the measurement of the light power corresponding to each first laser 111, the other first lasers 111 can be in a closed state except for the measured laser which is in an open state, so that the light signal reflected by the flow chamber 12 to the front light detector 22 is the light signal corresponding to the open first laser 111, and thus the front light detector 22 can obtain the corresponding first light power for each first laser 111 to obtain at least one first light power.

[0084] In the embodiments of the present application, each of the at least one first laser 111 corresponds to a switch, which is used to control the opening and closing of the corresponding first laser 111. For example, when the optical power of a certain first laser 111 is determined, the switch corresponding to the first laser 111 can be opened, and the switches corresponding to the other first lasers 111 can be closed.

[0085] For example, the at least one first laser 111 includes a first laser 1, a first laser 2, and a first laser 3. For the determination of the optical power corresponding to the first laser 1, the switch corresponding to the first laser 1 can be opened, and the switches corresponding to the first laser 2 and the first laser 3 can be closed. For the determination of the optical power corresponding to the first laser 2, the switch corresponding to the first laser 2 can be opened, and the switches corresponding to the first laser 1 and the first laser 3 can be closed. For the determination of the optical power corresponding to the first laser 3, the switch corresponding to the first laser 3 can be opened, and the switches corresponding to the first laser 1 and the first laser 2 can be closed. For the determination of the background optical power, the switches corresponding to the first laser 1, the first laser 2, and the first laser 3 can be closed.

[0086] In the embodiments of the present application, each of the at least one first laser 111 corresponds to a first optical power, and each of the at least one first laser 111 also corresponds to a preset optical power. It should be noted that if the models, batches, and manufacturing of the lasers are consistent, the same preset optical power can be used, that is, one preset optical power can be used for each of the at least one first laser 111. Of course, in order to be more accurate, and considering that the lasers of different batches, different models, and different manufacturing processes are different, different preset optical powers can be set for each of the at least one first laser 111. The preset optical power set for each of the at least one first laser 111 is also a standard optical power measured before the laser is manufactured.

[0087] In the embodiments of the present application, the controller 23 can be configured with the preset optical power corresponding to each of the at least one first laser 111. Thus, after the controller 23 receives the first optical power sent by the front light detector 22, the controller 23 can obtain at least one comparison result by comparing the first optical power with the corresponding preset optical power based on the relationship between the first optical power and the corresponding preset optical power.

[0088] For example, if the comparison result of the first laser 1 is that the corresponding first light power is less than the preset light power, it indicates that the laser attenuates or the flow chamber 12 has a problem; the comparison result of the first laser 2 is that the corresponding first light power is consistent with the preset light power, which indicates that the first laser 2 is normal; and the comparison result of the first laser 3 is that the corresponding first light power is greater than the preset light power, which indicates that the first laser 3 is abnormal. Based on the at least one comparison result, it can be determined that the first laser 1 attenuates, the flow chamber 12 is normal, the first laser 2 is normal, and the first laser 3 is abnormal.

[0089] In the embodiment of the present application, if the corresponding first light power of a single first laser 111 is greater than the preset light power, it indicates that the corresponding first laser has a problem and the flow chamber 12 is normal. Based on this determined result, the comparison results of other first lasers can be comprehensively judged to obtain the state monitoring result of the front light system 10.

[0090] In the embodiment of the present application, the controller 23 can obtain the state information of each first laser 111 and / or the state information of the flow chamber 12 based on the at least one comparison result.

[0091] In some embodiments, as shown in Figure 4 The reflector 21 includes at least one mirror group 211 corresponding to each of the at least one first laser 111. Each mirror group 211 is arranged between the corresponding first laser 111 and the flow chamber 12, and is used to reflect the light signal emitted by the corresponding first laser 111 to the front light detector 22.

[0092] In the embodiment of the present application, each mirror group 211 can include a long-focus lens 2111 and a turning mirror 2112. The light signal emitted by the first laser 111 passes through the long-focus lens 2111 to the turning mirror 2112, and then is transmitted to the cylindrical lens group 121, or the light signal emitted by the first laser 111 is reflected to the front light detector 22 after passing through the long-focus lens 2111 to the turning mirror 2112.

[0093] For example, the process of the light signal emitted by the first laser 111 passing through the turning mirror 2112 in the corresponding mirror group 211 to the cylindrical lens group 121 can pass through the turning mirror 2112 in the mirror group 211 of other first lasers 111.

[0094] In the embodiments of the present application, when measuring the optical power of each first laser 111, the other first lasers 111 are turned off, the emitted light signal of the measured first laser 111 reaches the turning mirror 2112 through the long-focus lens 2111, and then is reflected to the front light detector 22. Then, the controller 23 determines the state monitoring result of the front light system 10 based on the relationship between the optical power and the preset optical power. If the optical power is less than the preset optical power, it indicates that the measured first laser 111 is attenuated. If the optical power is greater than the preset optical power, it indicates that the measured first laser 111 is abnormal.

[0095] In the embodiments of the present application, the structure in Figure 5 and Figure 6 may also be used in combination to monitor the state of the front light system 10. As shown in Figure 7 , whether the first laser 111 is abnormal is determined according to the optical power of the first laser 111 detected by the first front light detector 231, and then whether the flow chamber of the first laser 111 is abnormal is determined according to the optical power of the first laser 111 detected by the second front light detector 232. For example, if the optical power of the first front light detector 231 indicates that the first laser 111 is normal, and if the optical power of the second front light detector 232 indicates that the first laser 111 or the flow chamber 12 is abnormal, the combination of the two can determine that the first laser 111 is normal and the flow chamber 12 is abnormal.

[0096] In this way, the state monitoring result of the front light system can be more accurately determined.

[0097] In some embodiments, as shown in Figure 8 , the front light detector 22 includes a current-to-voltage circuit 221, a filter circuit 222, and an analog-to-digital converter 223. The current-to-voltage circuit 221 is connected to the filter circuit 222 and is used to convert the light signal reflected by the reflector 21 into a voltage signal and input the converted voltage signal to the filter circuit 222. The filter circuit 222 is connected to the analog-to-digital converter 223 and is used to filter the converted voltage signal to obtain a filtered voltage signal. The analog-to-digital converter 223 is connected to the filter circuit 222 and is used to convert the filtered voltage signal into the optical power of the laser 11.

[0098] In the embodiments of the present application, the current-to-voltage circuit 221 can convert the light signal into a weak current signal based on a photodetector, and then convert the current signal into a voltage signal. For example, the photodetector can be a photodiode, an avalanche photodiode APD, or other devices that can convert a light signal into a current signal.

[0099] In the embodiments of this application, the filter circuit 222 can be a low-pass filter, a band-pass filter, or an active filter, used to filter the converted voltage signal to make the filtered voltage signal more stable. The selection of the filter circuit 222 can be based on actual needs and application scenarios.

[0100] In the embodiments of this application, the analog-to-digital converter 223 converts analog voltage signals into digital signals, facilitating digital signal processing. The resolution of the analog-to-digital converter 223 can be selected based on the accuracy requirements, specifically the number of bits in the ADC.

[0101] In the embodiments of this application, the current-to-voltage circuit 221, the filter circuit 222, and the analog-to-digital converter 223 are all linear. The filtered voltage signal obtained from the analog-to-digital converter 223 also has a linear relationship with the optical power, allowing the optical power of the laser 11 to be directly determined based on the filtered voltage signal. For example, the incident light and the current output by the photodetector are linear, and the current-to-voltage circuit 221, the filter circuit 222, and the analog-to-digital converter 223 are all linear circuits, thus the filtered voltage signal can directly characterize the optical power.

[0102] In the embodiments of this application, when performing optical power detection, only the DC signal needs to be observed. Through the front optical detection configuration such as the current-to-voltage circuit 221, the filter circuit 222, and the analog-to-digital converter 223, both DC accuracy and settling time can be optimized.

[0103] In some embodiments, such as Figure 9 As shown, the current-to-voltage circuit 221 may include: a first operational amplifier 2211 and a first resistor 2212; the first resistor 2212 is a variable resistor; the first resistor 2212 is connected in parallel to the inverting input terminal and the output terminal of the first operational amplifier 2211, and is used to adjust the amplification factor of the current-to-voltage circuit 221 to a preset amplification factor; the non-inverting input terminal of the first operational amplifier 2211 is grounded, and the output terminal is connected to the buffer circuit 224.

[0104] In the embodiments of this application, if the current corresponding to the light signal reflected by the reflector 21 is 1mW, the voltage obtained by the analog-to-digital converter 223 needs to be 3.3V. Based on this current and the final required voltage, the corresponding preset amplification factor can be determined.

[0105] For example, the larger the first resistor 2212 is, the higher the gain (amplification factor of the current-to-voltage circuit 221). However, in order to balance noise and bandwidth, the resistance value will have a preset range. If the amplification factor corresponding to the maximum resistance value of the variable resistor can be adjusted to the preset amplification factor, then the current value is directly converted into the corresponding voltage value based on the current-to-voltage circuit 221.

[0106] In the embodiments of this application, the current-to-voltage circuit 221 further includes a first phototube 2213 and a first capacitor 2214; the first phototube 2213 is grounded in the forward direction and connected to the inverting input terminal of the first operational amplifier 2211 in the reverse direction, and is used to convert the light signal emitted by the laser 11 into a current signal; the first capacitor 2214 and the first resistor 2212 are connected in parallel to form a feedback circuit.

[0107] In this way, voltage can be amplified to a preset factor without the need for a voltage amplifier circuit, simplifying the complexity of the circuit.

[0108] In some embodiments, the filter circuit 222 is an active filter circuit, such as... Figure 9 As shown, an active filter circuit 226 is provided. The active filter circuit 226 is disposed between the current-to-voltage circuit 221 and the analog-to-digital converter 223. The active filter circuit 226 includes a second operational amplifier 2221, a second resistor 2222, a second capacitor 2223, a third capacitor 2224, and a third resistor 2225. The second resistor 2222 and the third resistor 2225 are connected in series, with one end connected to the current-to-voltage circuit 221 and the other end input to the non-inverting input terminal of the second operational amplifier 2221. The second capacitor 2223 is connected in parallel with the third resistor 2225 and the second operational amplifier 2221. One end of the third capacitor 2224 is grounded, and the other end is connected to the non-inverting input terminal of the second operational amplifier 2221. The inverting input terminal of the second operational amplifier 2221 is connected to the output terminal, and the output terminal is connected to the analog-to-digital converter 223.

[0109] In the embodiments of this application, such as Figure 10 As shown, an RC filter circuit 227 (including a fourth resistor 2226 and a fourth capacitor 2227) is typically connected to the output of the current-to-voltage circuit 221 to filter the converted voltage signal. However, if the output resistor (fourth resistor 2226) of the current-to-voltage circuit 221 is directly connected to the input resistor (second resistor 2222) of the active filter circuit 226, a voltage divider will occur. For example, if the voltage flowing out of the current-to-voltage circuit 221 through the fourth resistor 2226 is 11V, then after the voltage is divided by the fourth resistor 2226 and the second resistor 2222, the voltage reaching the active filter circuit 226 will be lower than 11V. Therefore, a buffer circuit 224 can be provided between the current-to-voltage circuit 221 and the active filter circuit 226.

[0110] like Figure 11 As shown, the front photodetector 22 also includes a buffer circuit 224; the buffer circuit 224 is disposed between the current-to-voltage circuit 221 and the filter circuit 222, and is used to reduce the voltage drop between the output resistance of the current-to-voltage circuit 221 and the input resistance of the filter circuit 222, so as to input the converted voltage signal into the filter circuit 222.

[0111] In the embodiment of the present application, the buffer circuit 224 is configured to prevent the converted voltage signal output by the current-to-voltage circuit 221 from being divided by the transmission to the filter circuit 222, i.e., the active filter circuit 226.

[0112] For example, as shown in FIG. 2, the buffer circuit 224 includes a third operational amplifier 2241, wherein one end of a fourth resistor 2226 is connected to the current-to-voltage circuit 221, and the other end of the fourth resistor 2226 is connected to the non-inverting input terminal of the third operational amplifier 2241 and a fourth capacitor 2227, respectively; the other end of the fourth capacitor 2227 is grounded; and the output terminal of the third operational amplifier 2241 is connected to the inverting input terminal of the third operational amplifier 2241 and the active filter circuit 226, respectively. Figure 12

[0113] In the embodiment of the present application, the inverting input terminal and the output terminal of the third operational amplifier 2241 are shorted, and when the non-inverting input terminal inputs a signal, a voltage follower, i.e., the buffer circuit 224, is formed, so that the voltage drop between the fourth resistor 2226 and the second resistor 2222 is avoided, the voltage drop is avoided, and the voltage input to the active filter circuit 226 is ensured to be the converted voltage signal output by the current-to-voltage circuit 221.

[0114] In the embodiment of the present application, if the amplification factor, i.e., the gain of the current-to-voltage circuit 221 is less than the preset amplification factor, a voltage amplification circuit 225 can be connected after the current-to-voltage circuit 221.

[0115] In this way, the high input impedance of the buffer circuit 224 means that the current drawn from the signal source is very small. According to Ohm's law, when the input impedance is very high, even if the internal resistance of the signal source exists, the current flowing through the buffer circuit 224 will also be very small, which reduces the voltage drop on the signal, because the voltage drop is proportional to the product of the current and the internal resistance of the signal source, and the small current means that the voltage drop on the signal source is also small, thereby maintaining the voltage stability of the signal source. Low output impedance: the low output impedance of the buffer circuit 224 ensures that it can effectively drive the load. When the buffer circuit 224 provides a signal to the load, the low output impedance means that the output voltage is less affected by the change of the load resistance. According to the principle of voltage division, if the output impedance of the buffer circuit 224 is much smaller than the load resistance, the voltage on the load will be close to the output voltage of the buffer circuit 224, thereby avoiding the significant voltage drop caused by the load resistance.

[0116] For example, as shown in FIG. 2, the buffer circuit 224 includes a third operational amplifier 2241, wherein one end of a fourth resistor 2226 is connected to the current-to-voltage circuit 221, and the other end of the fourth resistor 2226 is connected to the non-inverting input terminal of the third operational amplifier 2241 and a fourth capacitor 2227, respectively; the other end of the fourth capacitor 2227 is grounded; and the output terminal of the third operational amplifier 2241 is connected to the inverting input terminal of the third operational amplifier 2241 and the active filter circuit 226, respectively. Figure 13 ​As shown, the front light detector 22 further comprises a voltage amplification circuit 225; the voltage amplification circuit 225 is arranged between the current-voltage conversion circuit 221 and the filter circuit 222, and is configured to perform secondary amplification on the converted voltage signal output by the current-voltage conversion circuit 221 in response to the amplification multiple of the current-voltage conversion circuit 221 being less than the preset amplification multiple.

[0117] In the embodiment of the present application, if the amplification multiple of the current-voltage conversion circuit 221 fails to reach the preset amplification multiple, secondary amplification is performed based on the voltage amplification circuit 225. For example, if the preset amplification multiple is 1000 and the amplification multiple of the current-voltage conversion circuit 221 is 960, the voltage amplification circuit 225 will amplify it to 1000 times, so that the amplification of the preset amplification multiple can be realized.

[0118] For example, as shown in FIG. 2, the voltage amplification circuit 225 comprises a fourth resistor 2226, a fourth capacitor 2227, a fifth resistor 2251, a fifth capacitor 2252, a sixth resistor 2253, and a third operational amplifier 2241; one end of the fourth resistor 2226 is connected with the current-voltage conversion circuit 221, and the other end is connected with the non-inverting input terminal of the third operational amplifier 2241 and the fourth capacitor 2227 respectively; the other end of the fourth capacitor 2227 is grounded; the fifth resistor 2251 is connected with the sixth resistor 2253 in series, and is connected with the fifth capacitor 2252 in parallel, and is connected with the inverting input terminal and the output terminal of the third operational amplifier 2241 in parallel; the other end of the sixth resistor 2253 is grounded; the output terminal of the third operational amplifier 2241 is connected with the active filter circuit 226. Figure 14 In the embodiment of the present application, the fourth resistor 2226 and the fourth capacitor 2227 are the RC filter circuit 227 discussed above, the fifth resistor 2251, the fifth capacitor 2252, and the sixth resistor 2253 are added on the basis of the third operational amplifier 2241, and the inverting input terminal and the output terminal of the third operational amplifier 2241 are no longer short-circuited, but are connected with the fifth resistor 2251, wherein the sixth resistor 2253 is a voltage dividing resistor, and forms a feedback loop with the fifth capacitor 2252 and the fifth resistor 2251.

[0119] In the embodiment of the present application, if the current-voltage conversion circuit 221 is connected with the voltage amplification circuit 225, a buffer circuit is not needed, that is, the buffer circuit 224 and the voltage amplification circuit 225 are arranged alternatively, if the amplification multiple of the current-voltage conversion circuit 221 reaches the preset amplification multiple, the buffer circuit 224 is selected, and if the amplification multiple of the current-voltage conversion circuit 221 fails to reach the preset amplification multiple, the voltage amplification circuit 225 can be selected.

[0120]

[0121] ​In the embodiments of the present application, the voltage amplification circuit 225 and the buffer circuit 224 can share some devices, such as the fourth resistor 2226, the fourth capacitor 2227, and the third operational amplifier 2241. A switch can be provided at the fifth resistor 2251. The switch can be a single-pole double-throw switch. When the buffer circuit 224 is used, the switch is connected to a wire. When the voltage amplification circuit 225 is used, the fifth resistor 2251 is connected to the circuit.

[0122] In the embodiments of the present application, a wire and a fifth resistor 2251 can be connected in parallel to the third operational amplifier 2241. A switch is provided on each branch. When the buffer circuit 224 is used, the switch on the wire branch is closed and the switch on the fifth resistor 2251 branch is opened. When the voltage amplification circuit 225 is used, the switch on the fifth resistor 2251 branch is closed and the switch on the wire branch is opened.

[0123] In the embodiments of the present application, switches can also be provided at the fifth capacitor 2252 and the sixth resistor 2253. When the buffer circuit 224 is used, the fifth capacitor 2252 and the sixth resistor 2253 are disconnected. When the voltage amplification circuit 225 is used, the fifth capacitor 2252 and the sixth resistor 2253 are connected to the circuit.

[0124] In the embodiments of the present application, the filter circuit 222 at least includes one of the following: a filter circuit 222 provided between the current-to-voltage conversion circuit 221 and the voltage amplification circuit 225 or the buffer circuit 224, for filtering the converted voltage signal; a filter circuit 222 provided between the voltage amplification circuit 225 or the buffer circuit 224 and the analog-to-digital converter 223, for filtering the preset voltage signal; and a filter circuit 222 connected to the analog-to-digital converter 223, for filtering the voltage signal input to the analog-to-digital converter 223.

[0125] In the embodiments of the present application, as shown in Figure 15 The filter circuit 222 can be an active filter circuit 226 provided between the voltage amplification circuit 225 and the analog-to-digital converter 223, for filtering the preset voltage signal. The filter circuit 222 can be the RC filter circuit 227 discussed above, which is provided between the current-to-voltage conversion circuit 221 and the voltage amplification circuit 225 or the buffer circuit 224, for filtering the converted voltage signal. The RC filter circuit 228 can also be provided between the analog-to-digital converter 223 and the active filter circuit 226, for filtering the voltage signal input to the analog-to-digital converter 223.

[0126] In the embodiments of the present application, as shown in Figures 7 to 15As shown, the filter circuit 222 can be an active filter circuit 226, which is arranged between the buffer circuit 224 and the analog-to-digital converter 223, and is configured to filter the preset voltage signal. The filter circuit 222 can be the RC filter circuit 227 discussed above, which is arranged between the current-to-voltage circuit 221 and the voltage amplification circuit 225 or the buffer circuit 224, and is configured to filter the converted voltage signal. The RC filter circuit 228 can also be arranged between the analog-to-digital converter 223 and the active filter circuit 226, and is configured to filter the voltage signal input to the analog-to-digital converter 223.

[0127] In the embodiment of the present application, the RC filter circuit 228 includes a seventh resistor 2281 and a sixth capacitor 2282.

[0128] In the embodiment of the present application, at least one filter circuit 222 is arranged in the front light detector 22, which can realize multi-stage filtering and improve the filtering performance.

[0129] Exemplarily, the basic parameters of the devices involved in the above Figure 16 may be as follows: the first capacitor 2214 can be 10 pF, the first operational amplifier 2211 can be AD8033, the first resistor 2212 can be 10 kΩ, the second operational amplifier 2221 can be AD8033, the second resistor 2222 can be 100 kΩ, the second capacitor 2223 can be 500 nF, the third capacitor 2224 can be 500 nF, the third resistor 2225 can be 100 kΩ, the fourth resistor 2226 can be 10 kΩ, the fourth capacitor 2227 can be 1 μF, the third operational amplifier 2241 can be AD8033, the fifth resistor 2251 can be 10 kΩ, the fifth capacitor 2252 can be 10 pF, and the sixth resistor 2253 can be 10 kΩ.

[0130] As shown in Figure 17 , the present application provides a flowchart of an exemplary state monitoring method of a front light system, which is applied to a state monitoring system of the front light system. The state monitoring system includes a reflector, a front light detector, and a controller. The reflector is arranged in the front light system, faces the laser, and is at a preset angle with the forward scattering detector. The front light detector is connected with the controller. The exemplary state monitoring method of the front light system includes the following steps S1601 to S1603:

[0131] In step S1601, the reflector reflects the light signal emitted by the laser to the front light detector.

[0132] In the embodiments of the present application, the front scattered light of the light signal emitted by the laser in the front light system enters the receiver of the forward scattering detector, and the side scattered light enters the lens in the front light system and reaches the rear light system through the lens.

[0133] In the embodiments of the present application, the scattered light entering the receiver is reflected to the front light detector through the reflector by installing the reflector on the side of the laser before the receiver of the forward scattering detector in the front light system.

[0134] In the embodiments of the present application, the light signal emitted by the laser can also be reflected to the front light detector based on the reflector between the laser and the flow chamber in the front light system.

[0135] In the embodiments of the present application, the state monitoring of the front light system is performed when the front light system is in operation, that is, there are cells to be detected in the flow chamber.

[0136] Step S1602, the front light detector converts the light signal reflected by the reflector into the optical power of the laser, and transmits the optical power to the controller.

[0137] In the embodiments of the present application, the front light detector converts the light signal reflected by the reflector into the optical power of the laser, and then transmits the converted optical power to the controller.

[0138] Step S1603, the controller determines the state monitoring result of the front light system based on the relationship between the optical power and the preset optical power.

[0139] In the embodiments of the present application, after receiving the optical power, the controller can determine the state monitoring result of the front light system based on the relationship between the optical power and the previously stored preset optical power corresponding to the optical power.

[0140] In the embodiments of the present application, if the optical power obtained by the front light detector of the forward scattering detector is less than the preset optical power, it indicates that the laser in the front light system is attenuated or the flow chamber is abnormal. For example, the abnormality of the flow chamber can be the abnormality of the inner wall or the outer wall of the flow chamber, at which time the laser can be replaced or the flow chamber can be cleaned. If it is greater than the preset optical power, it indicates that the laser is abnormal, and the laser can be replaced.

[0141] In the embodiments of the present application, if the optical power obtained by the front light detector after the turning mirror (located between the flow chamber and the laser) is less than the preset optical power, it indicates that the laser in the front light system is attenuated, at which time the laser can be replaced. If it is greater than the preset optical power, it indicates that the laser is abnormal, and the laser can be replaced.

[0142] In the embodiments of the present application, the light power obtained by the front light detector of the forward scattering detector and the light power obtained by the front light detector after the turning mirror (between the flow chamber and the laser) can be used to comprehensively determine the relationship with the preset light power. For example, if the light power obtained by the front light detector after the turning mirror (between the flow chamber and the laser) is equal to the preset light power, it indicates that the laser is normal, and if the light power obtained by the front light detector of the forward scattering detector is less than the preset light power, it indicates that the flow chamber is abnormal, and the flow chamber can be cleaned.

[0143] In this way, the laser or the flow chamber can be monitored, and the monitoring efficiency is improved.

[0144] In some embodiments, as shown in FIG. 17, the laser in the front light system includes at least one first laser, and the state monitoring system of the front light system can further perform the following steps S1701 and S1702: ​

[0145] In step S1701, the front light detector determines the corresponding first light power when each of the at least one first laser works alone, and obtains at least one first light power.

[0146] In the embodiments of the present application, the laser includes at least one first laser, and for each of the at least one first laser, a separate measurement can be performed to obtain the corresponding first light power of each first laser, and then at least one first light power is obtained.

[0147] In step S1702, the controller compares each of the at least one first light power with the preset light power to obtain at least one comparison result, and determines the state information of each first laser in the front light system and / or the state information of the flow chamber based on the at least one comparison result.

[0148] In the embodiments of the present application, the controller compares each of the at least one first light power with the corresponding preset light power after receiving it. For example, the controller stores the corresponding preset light power of each first laser, and then obtains the corresponding preset light power from the corresponding relationship after receiving the corresponding first light power for comparison, and obtains the comparison result. In this way, at least one comparison result can be obtained.

[0149] In the embodiments of the present application, the controller uploads the state monitoring result represented by each comparison result to the upper computer and provides it to the operator based on each comparison result and the state detection result. The operator can perform fault diagnosis based on each comparison result and the state detection result. Of course, the corresponding solution measures for each comparison result can also be stored and then provided to the operator synchronously. ​

[0150] Exemplarily, the monitoring process of the front light system is that the light power of each laser after passing through the flow chamber is detected independently, and only one laser is turned on each time. In addition, the background of the laser is also detected when all the lasers are turned off, which is used as background subtraction. Before the instrument is shipped, the background (light and electricity) and the light power of the laser after passing through the flow chamber are monitored, and the processed data are saved. The instrument automatically measures these data each time it is self-checked, and compares them with the data before shipment to see if they are within a certain tolerance (such as 5%). If they are out of the range, it means that the front light system has a problem, and the program will make a judgment and give a prompt for troubleshooting.

[0151] The application provides a state monitoring method of a front light system, which is applied to a state monitoring system of the front light system. The state monitoring system comprises a reflector, a front light detector and a controller. The reflector is arranged in the front light system, faces the laser and is at a preset angle with the forward scattering detector. The front light detector is connected with the controller. The method comprises the following steps: the reflector reflects the light signal emitted by the laser to the front light detector; the front light detector converts the light signal reflected by the reflector into the light power of the laser, and transmits the light power to the controller; and the controller determines the state monitoring result of the front light system based on the relationship between the light power and a preset light power. The state monitoring method of the front light system provided by the application can realize the acquisition of the light power corresponding to the laser based on the reflector and the front light detector, so as to monitor the state of the front light system and improve the monitoring efficiency.

[0152] It should be understood that the "one embodiment" or "an embodiment" mentioned throughout the specification means that the specific features, structures or characteristics related to the embodiment are included in at least one embodiment of the application. Therefore, "in one embodiment" or "in an embodiment" appearing throughout the specification does not necessarily refer to the same embodiment. In addition, these specific features, structures or characteristics can be combined in one or more embodiments in any suitable manner. It should be understood that in various embodiments of the application, the size of the serial number of each step / process does not mean the execution order, and the execution order of each step / process should be determined according to its function and inherent logic, and should not constitute any limitation on the implementation process of the embodiments of the application. The serial number of the above embodiments of the application is only for description, not representing the advantages and disadvantages of the embodiments.

[0153] It should be noted that, in the present document, the terms "comprising", "comprising" or any other variant thereof are intended to cover non-exclusive inclusions, so that a process, method, article, or apparatus that comprises a list of elements not only includes those elements, but also includes other elements not expressly listed or inherent to such process, method, article, or apparatus. Without more limitations, the element defined by the statement "comprising a" does not exclude the presence of additional identical elements in the process, method, article, or apparatus that includes the element.

[0154] The above description is only an embodiment of the present application, but the protection scope of the present application is not limited thereto, and any person skilled in the art can easily think of changes or replacements within the technical scope disclosed by the present application, which should be covered by the protection scope of the present application.

Claims

1. A front light system status monitoring system, characterized by, The state monitoring system comprises a reflector, a front light detector and a controller; The reflector is located in the front light system, faces the laser and is at a preset angle with the direction of the light signal emitted by the laser, and is used for reflecting the light signal emitted by the laser to the front light detector; The front light detector is connected with the controller, and is used for converting the light signal reflected by the reflector into the optical power of the laser, and transmitting the optical power to the controller; The controller is used for determining the state monitoring result of the front light system based on the relationship between the optical power and the preset optical power.

2. The state monitoring system of the front light system according to claim 1, wherein the reflector is arranged on the side of the front light system facing the laser and at the preset angle with the forward scattering detector, and is used for reflecting the light signal emitted by the laser to the front light detector after passing through the flow chamber. The laser in the front light system comprises at least one first laser; 3. The front light system status monitoring system of claim 2, wherein, The front light detector is used for determining the corresponding first optical power when each first laser in the at least one first laser works alone, and obtaining at least one first optical power; The controller is used for comparing each first optical power in the at least one first optical power with the preset optical power to obtain at least one comparison result, and determining the state information of each first laser in the front light system and / or the state information of the flow chamber based on the at least one comparison result. The reflector comprises at least one mirror group corresponding to the at least one first laser; 4. The front light system status monitoring system of claim 3, wherein, Each mirror group in the at least one mirror group is arranged between the corresponding first laser in the at least one first laser and the flow chamber, and is used for reflecting the light signal emitted by the corresponding first laser to the front light detector. The front light detector comprises a current-to-voltage circuit, a filter circuit and an analog-to-digital converter; 5. The front light system status monitoring system according to any one of claims 1 to 4, characterized in that, The current-to-voltage circuit is connected with the filter circuit, and is used for converting the light signal reflected by the reflector into a voltage signal, and inputting the converted voltage signal into the filter circuit; The filter circuit is connected with the analog-to-digital converter, and is used for filtering the converted voltage signal to obtain a filtered voltage signal; The analog-to-digital converter is connected with the filter circuit, and is used for converting the filtered voltage signal into the optical power of the laser. The front light detector further comprises a buffer circuit; 6. The front light system status monitoring system of claim 5, wherein, The buffer circuit is arranged between the current-to-voltage circuit and the filter circuit, and is used for reducing the voltage drop between the output resistance of the current-to-voltage circuit and the input resistance of the filter circuit, so as to input the converted voltage signal into the filter circuit. The front light detector further comprises a voltage amplification circuit; 7. The front light system status monitoring system of claim 5, wherein, The voltage amplification circuit is arranged between the current-to-voltage circuit and the filter circuit, and is used for performing secondary amplification on the converted voltage signal output by the current-to-voltage circuit in response to the amplification multiple of the current-to-voltage circuit being less than a preset amplification multiple. A first operational amplifier and a first resistor; 8. The front light system status monitoring system of claim 6, the current-to-voltage circuit comprising: ​ The first resistor is a variable resistor; The first resistor is connected in parallel to the inverting input and the output of the first operational amplifier, and is used to adjust the amplification of the current-to-voltage circuit to a preset amplification; The non-inverting input of the first operational amplifier is connected to the ground, and the output is connected to the buffer circuit.

9. The state monitoring system of the front light system according to claim 5, wherein the filter circuit comprises at least one of the following: The filter circuit is arranged between the current-to-voltage circuit and the voltage amplification circuit or the buffer circuit, and is used to filter the converted voltage signal; The filter circuit is arranged between the voltage amplification circuit or the buffer circuit and the analog-to-digital converter, and is used to filter the preset voltage signal; The filter circuit is connected to the analog-to-digital converter, and is used to filter the voltage signal input into the analog-to-digital converter.

10. The state monitoring system of the front light system according to any one of claims 1 to 9, wherein the front light system monitored by the state monitoring system is a system arranged in a flow cytometer to provide a light signal.

11. A method of monitoring the condition of a front light system, characterized by A state monitoring system applied to a front light system, the state monitoring system comprising a reflector, a front light detector, and a controller; the reflector is located in the front light system, and is directed towards a laser and forms a preset angle with a direction in which the laser emits a light signal; the front light detector is connected to the controller; the method comprises: The reflector reflects the light signal emitted by the laser to the front light detector; The front light detector converts the light signal reflected by the reflector into a light power of the laser, and transmits the light power to the controller; The controller determines a state monitoring result of the front light system based on a relationship between the light power and a preset light power.

12. A method of condition monitoring of a light system according to claim 11, characterized in that The laser in the front light system comprises at least one first laser; the method further comprises: The front light detector determines a corresponding first light power when each of the at least one first laser works individually, and obtains at least one first light power; The controller compares each of the at least one first light power with the preset light power, obtains at least one comparison result, and determines state information of each of the first lasers and / or state information of the flow chamber based on the at least one comparison result.

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