Accurate cell assembly system based on synchronous sound tweezers

Abstract

The invention discloses a precise cell assembly system based on synchronous sound tweezers, and relates to the technical field of biomedical engineering. The device comprises a signal adjusting module for generating a high-frequency signal and amplifying the high-frequency signal, a sound vortex transducer module arranged on the signal adjusting module and used for generating a sound vortex sound field, and a micro-channel arranged on the sound vortex transducer module and used for capturing cells and then assembling the cells, the moving device is arranged on the sound vortex transducer module and used for driving the sound vortex transducer module to accurately move, the sound vortex transducer module is obliquely arranged at an oblique incidence angle, and capturing and assembling of cells are achieved through sound tweezers. The method can improve the accuracy of cell assembly and reduce the cost, and has economical efficiency.

Description

Technical Field

[0001] This invention relates to the field of biomedical engineering technology, and in particular to a precise cell assembly system based on synchronous acoustic tweezers. Background Technology

[0002] Precise cell assembly is the foundation of technologies such as tissue engineering and assisted reproduction. Compared with optical tweezers, acoustic tweezers have advantages such as stronger control over cells, lower cost, and better biocompatibility, and have broad application prospects.

[0003] Acoustic vortices are special types of sound waves carrying orbital angular momentum, characterized by a zero-pressure center and a helical wavefront. While vortices possess the ability to selectively manipulate human cells, their acoustic fields repel cells outside the trapping potential well, hindering the possibility of two cells continuously approaching each other for cell assembly. This significantly limits the application of vortex acoustic tweezers in the biomedical field. Existing research suggests that an assembly channel can be opened between two synchronized acoustic vortices using the destructive interference effect, thereby enabling the assembly of two cells. However, in practical applications, the physical size limitations of the two transducers prevent continuous approach, further restricting the practicality of this method. Summary of the Invention

[0004] In view of the above-mentioned deficiencies of the prior art, the technical problem to be solved by the present invention is how to achieve precise cell assembly based on acoustic tweezers technology, simplify the system structure, and reduce the system cost.

[0005] To achieve the above objectives, the present invention provides a cell precision assembly system based on synchronous acoustic tweezers, comprising a signal conditioning module for generating and amplifying high-frequency signals, an acoustic vortex transducer module disposed on the signal conditioning module for generating an acoustic vortex sound field, a microchannel disposed on the acoustic vortex transducer module for pre-capturing and then assembling cells, and a moving device disposed on the acoustic vortex transducer module for driving the acoustic vortex transducer module to move accurately. The acoustic vortex transducer module is tilted at an oblique incident angle, and cell capture and precise assembly are achieved through acoustic tweezers.

[0006] Furthermore, the oblique angle of incidence is 60°.

[0007] Furthermore, the signal conditioning module includes a signal generator for generating a high-frequency signal and a power amplifier for amplifying the high-frequency signal. The amplified high-frequency signal is used to drive the acoustic vortex transducer module to generate the desired acoustic vortex sound field.

[0008] Furthermore, the acoustic vortex transducer module includes two acoustic vortex transducers for generating mutually interfering synchronous acoustic vortex sound fields, and the two acoustic vortex transducers are symmetrically arranged at an oblique incident angle of 60°.

[0009] Furthermore, the operating frequency of the acoustic vortex transducers is 5 Hz. MHz The corresponding wavelength is λ =300 μm Topological load number l Both are 1, and the two acoustic vortex transducers respectively revolve around y The axis rotates ±60°.

[0010] Furthermore, the acoustic vortex transducer is a planar acoustic vortex transducer.

[0011] Furthermore, the acoustic vortex transducer is fabricated using MEMS technology.

[0012] Furthermore, the microchannel contains a suspension of cells to be assembled, and the acoustic vortex sound field generated by the acoustic vortex transducer module enables the cells to be captured and assembled within the microchannel.

[0013] Furthermore, the mobile device assembles cells by controlling the precise movement of the acoustic vortex transducer module on the slide rail via a motor.

[0014] Furthermore, the size of the cells to be assembled was 5. μm Its size is much smaller than the wavelength of sound waves, and the density of cells is ρ p =1100 kg / m 3 The speed of sound is c p =1500 m / s The liquid medium is water with a density of ρ 0 =1000 kg / m 3 The speed of sound is c 0 =1500 m / s .

[0015] Compared with the prior art, the present invention has the following advantages: This invention employs a "capture-then-assemble" strategy. By designing an oblique incidence angle, precise assembly of two cells can be achieved simply by adjusting the emission angle of the acoustic vortex transducer. This overcomes the challenge in existing research where the physical dimensions of the two transducers limit their ability to continuously approach each other for cell assembly, effectively reducing production costs and demonstrating practicality and economy. The planar miniature acoustic vortex device, fabricated using MEMS technology, is compatible with platforms such as microscopes, coverslips, and microchannels, and is easily integrated.

[0016] The following will further explain the concept, specific structure, and technical effects of the present invention in conjunction with the accompanying drawings, so as to fully understand the purpose, features, and effects of the present invention. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the cell precision assembly system based on synchronous acoustic forceps according to an embodiment of the present invention; Figure 2 The image shows the sound field amplitude and phase diagrams of a single acoustic vortex incident at a 60° oblique angle according to an embodiment of the present invention. Figure 3 This is a schematic diagram illustrating the process of how, in an embodiment of the present invention, when two obliquely incident acoustic vortices approach each other, the interference and destructive effect of the sound field forms an assembly channel between the two acoustic vortices, allowing the cells located at the centers of the two vortices to approach each other under the action of acoustic radiation force to achieve assembly. Figure 4 For the purposes of embodiments of the present invention Gor'kov The transverse acoustic radiation force experienced by two cells in an obliquely incident composite acoustic vortex acoustic field, obtained from theoretical calculations. F x and axial acoustic radiation force F z A graph showing the relationship between position and location. Detailed Implementation

[0018] The preferred embodiments of the present invention are described below with reference to the accompanying drawings to make the technical content clearer and easier to understand. The present invention can be embodied in many different forms, and the scope of protection of the present invention is not limited to the embodiments mentioned herein.

[0019] In the accompanying drawings, components with the same structure are indicated by the same numerical designation, and components with similar structures or functions are indicated by similar numerical designations. The dimensions and thicknesses of each component shown in the drawings are arbitrary, and the present invention does not limit the dimensions and thicknesses of each component. To make the illustrations clearer, the thickness of some components has been appropriately exaggerated in the drawings.

[0020] like Figure 1 The diagram shows the structure of the cell precision assembly system based on synchronous acoustic forceps of the present invention. The system includes: Signal conditioning module: includes a signal generator and a power amplifier. The signal generator generates a high-frequency signal, which is amplified by the power amplifier to drive the acoustic vortex transducer to generate the desired acoustic vortex sound field.

[0021] Microchannel: Located above the acoustic vortex transducer, it contains a suspension of cells to be assembled. The acoustic vortex field generated by the acoustic vortex transducer enables the cells to be captured and assembled in the microchannel.

[0022] Acoustic Vortex Transducer Module: Includes two acoustic vortex transducers used to generate synchronous acoustic vortex sound fields that can interfere with each other.

[0023] Mobility device: The two acoustic vortex transducers can be accurately moved on the slide rail by motor control, thereby realizing cell assembly.

[0024] like Figure 2 The image shows the sound field amplitude and phase when a single acoustic vortex is incident at a 60° angle. Due to the oblique incidence... xy The acoustic vortex sound field in the plane is elliptical rather than circular. The innermost zero-intensity region of the acoustic vortex sound field is the cell capture region. Two obliquely incident acoustic vortex sound fields can each capture one cell first, preparing for the assembly of the next two cells.

[0025] Figure 3 The diagram illustrates how, when two obliquely incident acoustic vortices approach each other, the destructive interference effect of the acoustic vortex sound fields gradually creates an assembly channel between the two vortices. This allows cells located at the centers of the two vortices to continuously approach each other under the influence of acoustic radiation force, ultimately achieving cell assembly. This acoustic radiation force utilizes well-known theories in the field of acoustic manipulation. Gor'kov Theoretically, the calculation yields the following expression: (0) (2) (3) (4) In the above formula, F rad Representing acoustic radiation force, its components can be expressed as: F x , F y and F z , U For Gor'kov's advantage. u 1 and p 1 These are the first-order particle vibration velocity field and the first-order sound pressure field in the liquid medium, respectively. ρ0 and c 0 These are the density of the liquid medium and the speed of sound, respectively. ρ p and c p These are the density and sound velocity of the cells to be manipulated in the acoustic vortex sound field, respectively. a The radius of the cell to be manipulated is given by <·>, where <·> represents the time average of the physical quantities within the parentheses. f 1 and f 2 These are the monopole coefficient and dipole coefficient of a cell in a liquid medium under acoustic field incidence, respectively.

[0026] In use, both acoustic vortex devices operate at a frequency of 5 Hz. MHz The corresponding wavelength is λ =300 μm Topological load number l Both are 1, and the two acoustic vortex devices revolve around... y The axis is rotated ±60°, and the size of the cells to be assembled is 5. μm Its size is much smaller than the wavelength of sound waves, and the density of cells is ρ p =1100 kg / m 3 The speed of sound is c p =1500 m / s The liquid medium is water, with a density of... ρ 0 =1000 kg / m 3 The speed of sound is c 0 =1500 m / s .

[0027] Figure 4 The following is a demonstration of the use of Gor'kov The transverse acoustic radiation force experienced by two cells in an obliquely incident composite acoustic vortex acoustic field, obtained from theoretical calculations. F x and axial acoustic radiation force F z Relationship with changes in position, Figure 4 This clearly reflects the process by which cells can continuously approach each other under the influence of acoustic radiation force and eventually assemble.

[0028] This invention employs a "capture-then-assemble" strategy. By designing an oblique incidence angle, the assembly of two cells can be achieved simply by adjusting the emission angle of the acoustic vortex transducer. This overcomes the challenge in existing research where the physical dimensions of the two transducers limit their ability to continuously bring the cells closer together for assembly, demonstrating its practicality. Furthermore, the planar miniature acoustic vortex device fabricated using MEMS technology is compatible with platforms such as microscopes, coverslips, and microchannels, exhibiting ease of integration.

[0029] The preferred embodiments of the present invention have been described in detail above. It should be understood that those skilled in the art can make numerous modifications and variations based on the concept of the present invention without creative effort. Therefore, all technical solutions that can be obtained by those skilled in the art based on the concept of the present invention through logical analysis, reasoning, or limited experimentation on the basis of existing technology should be within the scope of protection defined by the claims.

Claims

1. A cell assembly system based on synchronized acoustic forceps, characterized in that, The device includes a signal conditioning module for generating and amplifying high-frequency signals, an acoustic vortex transducer module for generating an acoustic vortex sound field disposed on the signal conditioning module, a microchannel for capturing and assembling cells disposed on the acoustic vortex transducer module, and a moving device for driving the acoustic vortex transducer module to move accurately disposed on the acoustic vortex transducer module. The acoustic vortex transducer module is tilted at an oblique incident angle, and cell capture and precise assembly are achieved by acoustic tweezers.

2. The cell assembly system based on synchronous acoustic forceps as described in claim 1, characterized in that, The oblique angle of incidence is 60°.

3. The cell assembly system based on synchronous acoustic forceps as described in claim 2, characterized in that, The signal conditioning module includes a signal generator for generating high-frequency signals and a power amplifier for amplifying the high-frequency signals. The amplified high-frequency signals are used to drive the acoustic vortex transducer module to generate the desired acoustic vortex sound field.

4. The cell precision assembly system based on synchronous acoustic forceps as described in claim 2, characterized in that, The acoustic vortex transducer module includes two acoustic vortex transducers for generating mutually interfering synchronous acoustic vortex sound fields, and the two acoustic vortex transducers are symmetrically arranged at an oblique incident angle of 60°.

5. The cell assembly system based on synchronous acoustic forceps as described in claim 4, characterized in that, The operating frequency of the acoustic vortex transducers is 5. MHz The corresponding wavelength is λ =300 μm Topological load number l Both are 1, and the two acoustic vortex transducers respectively revolve around y The axis rotates ±60°.

6. The cell precision assembly system based on synchronous acoustic forceps as described in claim 4, characterized in that, The acoustic vortex transducer is a planar acoustic vortex transducer.

7. The cell precision assembly system based on synchronous acoustic forceps as described in claim 4, characterized in that, The acoustic vortex transducer is fabricated using MEMS technology.

8. The cell precision assembly system based on synchronous acoustic forceps as described in claim 2, characterized in that, The microchannel contains a suspension of cells to be assembled, and the acoustic vortex sound field generated by the acoustic vortex transducer module enables the cells to be captured and then assembled within the microchannel.

9. The cell precision assembly system based on synchronous acoustic forceps as described in claim 2, characterized in that, The mobile device assembles cells by controlling the precise movement of the acoustic vortex transducer module on the slide rail via a motor.

10. The cell precision assembly system based on synchronous acoustic forceps as described in any one of claims 1-9, characterized in that, The size of the cells to be assembled is 5. μm Its size is much smaller than the wavelength of sound waves, and the density of cells is ρ p =1100 kg / m 3 The speed of sound is c p =1500 m / s The liquid medium is water with a density of ρ 0 =1000 kg / m 3 The speed of sound is c 0 =1500 m / s .

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