Matrix nutrient soil and preparation and application thereof

Abstract

The invention provides matrix nutrient soil as well as preparation and application thereof. The matrix nutrient soil is prepared from 10 to 35 parts of sugarcane leaf powder, 30 to 55 parts of vermiculite, 0.01 to 0.06 part of compound microbial agent, 3 to 15 parts of yeast metabolite, 10 to 30 parts of yeast residue, 1 to 10 parts of sugar residue and 0.04 to 0.09 part of raspberry ketone. The matrix nutrient soil provided by the invention is soft, small in density, strong in permeability, high in organic matter content and lasting in fertilizer efficiency, can be beneficial to improving the seed germination rate and promoting crop growth, is wide in raw material source and environment-friendly, and can be beneficial to realizing low-carbon circulation and pollution-free.

Description

Technical Field

[0001] This invention relates to the field of soil technology, specifically to a substrate nutrient soil and its preparation and application. Background Technology

[0002] With the rise of hydroponics technology, the scale and development of flower cultivation have made significant progress. As the country with the largest flower cultivation area in the world, China not only has a huge planting area but also a vast consumer market. Furthermore, it has offered suggestions for the continuous improvement of the flower industry system, guiding the industry towards standardization, large-scale operation, and regulation.

[0003] Soilless cultivation using substrate nutrient soil is conducted under controlled conditions and can completely replace soil cultivation. Various soilless cultivation methods suitable for China's national conditions are being applied in agriculture, becoming an effective way to thoroughly solve problems such as soil acidification, salinization, compaction, and continuous cropping obstacles. Furthermore, the nutrient soil is specially formulated to meet the growth and development needs of seedlings, containing various mineral nutrients, and has the advantages of being loose and well-aerated, having strong water and fertilizer retention capacity, and being free from pests and diseases.

[0004] The substrate nutrient soil should have a certain pH level, no odor, and must be fully fermented and decomposed. In order to meet the needs of plant growth and development, it is necessary to prepare nutrient soil that is nutrient-rich, rich in humus, retains fertilizer and water, and has good permeability. Summary of the Invention

[0005] The nutrient composition of existing potting soil is relatively simple, making it prone to nutrient deficiency.

[0006] To address the problems in the prior art, this invention provides a substrate nutrient soil, its preparation, and its application.

[0007] Specifically, the present invention provides the following technical solution: In a first aspect, the present invention provides a substrate nutrient soil, which, by weight, comprises: 10-35 parts sugarcane leaf powder, 30-55 parts vermiculite, 0.01-0.06 parts compound microbial agent, 3-15 parts yeast metabolites, 10-30 parts yeast residue, 1-10 parts sugar residue, and 0.04-0.09 parts raspberry ketone.

[0008] Preferably, the substrate nutrient soil comprises, by weight: 17-27 parts sugarcane leaf powder, 38-45 parts vermiculite, 0.03-0.06 parts compound microbial agent, 3-7 parts yeast metabolites, 19-30 parts yeast residue, 5-10 parts sugar residue, and 0.04-0.07 parts raspberry ketone.

[0009] Preferably, the number of viable Bacillus subtilis bacteria in the compound microbial agent is 150-350 billion per gram of compound microbial agent.

[0010] Preferably, the sugar residue is sugarcane bagasse.

[0011] Secondly, the present invention provides a method for preparing a substrate nutrient soil, the preparation method comprising the following steps: (1) Mixing: Sugar residue, yeast residue, yeast metabolites, sugarcane leaf powder and compound microbial agent are mixed to obtain a mixture; (2) Stacking: Stack the mixture obtained in step (1); (3) Turning over: The mixture obtained in step (2) is turned over; (4) Fermentation: Ferment the mixture after turning over in step (3); (5) Crushing: The fermented mixture obtained in step (4) is crushed to obtain the target mixture; (6) Mixing: The target mixture obtained in step (5) is mixed with vermiculite and raspberry ketone to obtain substrate nutrient soil.

[0012] Preferably, in step (3), turning over is performed when the temperature of the mixed material after stacking obtained in step (2) reaches 60-80℃.

[0013] Preferably, in step (4), the mixture after the second turning in step (3) is placed naturally for fermentation, and the fermentation temperature is stabilized below 40°C. Preferably, the cycle fermentation is completed at 35-40°C.

[0014] Preferably, the natural placement time is 5-10 days.

[0015] Thirdly, the present invention provides an application of the substrate nutrient soil described above or the substrate nutrient soil prepared by the preparation method described above in the cultivation of Cucurbitaceae plants, preferably in the cultivation of cucumbers.

[0016] Fourthly, the present invention provides the application of the substrate nutrient soil described above or the substrate nutrient soil prepared by the preparation method described above in the cultivation of Solanaceae plants, preferably in the cultivation of eggplant, chili pepper and tomato or more thereof.

[0017] Fifthly, the present invention provides an application of the substrate nutrient soil described above or the substrate nutrient soil prepared by the preparation method described above in the cultivation of cruciferous plants, preferably in the cultivation of radishes.

[0018] Sixthly, the present invention provides the application of the substrate nutrient soil described above or the substrate nutrient soil prepared by the preparation method described above in the cultivation of gramineous plants, preferably in the cultivation of wheat.

[0019] In a seventh aspect, the present invention provides the application of the substrate nutrient soil described above or the substrate nutrient soil prepared by the preparation method described above in the cultivation of Convolvulaceae plants, preferably in the cultivation of water spinach.

[0020] The technical solution provided by this invention has the following beneficial technical effects: (1) The substrate nutrient soil provided by the present invention is loose and has low density, strong permeability, high organic matter content, and long-lasting fertilizer effect, which can help improve seed germination rate and promote crop growth. Moreover, the raw materials are widely available and environmentally friendly, which can help achieve low-carbon, circular and pollution-free operation. (2) The substrate nutrient soil provided by the present invention provides a large number of elements required for crop growth, has high sustainability, does not lack nutrients, and can thus promote the growth of crop roots and improve absorption and utilization rate. (3) The preparation method provided by the present invention has a fast fermentation temperature, a long high temperature duration, and a relatively thorough decomposition. It also has good control over the uniformity of material particles in the later stage, and the fermentation cycle is short and the production process is simple. Attached Figure Description

[0021] Figure 1 The graph shows the germination rate of radishes, tomatoes, chili peppers, eggplants, and cucumbers in the substrate nutrient soil.

[0022] Figure 2 This is a statistical result of the germination rate data of radish, tomato, chili pepper, eggplant and cucumber in substrate nutrient soil.

[0023] Figure 3 This is a diagram showing the growth of wheat under the influence of the substrate nutrient soil.

[0024] Figure 4 This is a statistical result of the effect of substrate nutrient soil on the number of wheat leaves and plant height.

[0025] Figure 5 This is a diagram showing the growth of water spinach in the substrate nutrient soil.

[0026] Figure 6 The data on the number of leaves and plant height of water spinach are statistical results based on the substrate nutrient soil. Detailed Implementation

[0027] To better understand the above technical solutions, the technical solutions of the present invention will be clearly and completely explained below in conjunction with specific embodiments. It should be noted that the content of the specific embodiments is only a specific implementation and explanation of the technical solutions of the present invention, and should not be construed as a limitation on the scope of protection of the present invention.

[0028] This invention provides a substrate nutrient soil comprising sugarcane leaf powder, vermiculite, compound microbial inoculant, yeast metabolites, yeast residue, sugar residue, and raspberry ketone. This substrate nutrient soil is rich in nutrients, and the nutrients in each component work synergistically, making it less likely for cultivated crops to experience nutrient deficiency.

[0029] In one specific embodiment of the present invention, a substrate nutrient soil is provided, which, by weight, comprises: 10-35 parts sugarcane leaf powder, 30-55 parts vermiculite, 0.01-0.06 parts compound microbial agent, 3-15 parts yeast metabolites, 10-30 parts yeast residue, 1-10 parts sugar residue, and 0.04-0.09 parts raspberry ketone.

[0030] Preferably, the substrate nutrient soil contains 10-35 parts by weight of sugarcane leaf powder. For example, in some specific embodiments, the sugarcane leaf powder may contain 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, or 35 parts of sugarcane leaf powder, or sugarcane leaf powder within the numerical range formed by any two of the above specific values ​​as endpoints.

[0031] Preferably, the substrate nutrient soil contains 30-55 parts by weight of vermiculite. For example, in some specific embodiments, the vermiculite may contain 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, or 55 parts of vermiculite, or vermiculite within the numerical range formed by any two of the above specific values ​​as endpoints.

[0032] Preferably, the substrate nutrient soil contains 0.01-0.06 parts by weight of a compound microbial agent. For example, in some specific embodiments, the compound microbial agent may contain 0.01 parts, 0.02 parts, 0.03 parts, 0.04 parts, 0.05 parts, or 35 parts, 0.06 parts of a compound microbial agent, or contain a compound microbial agent within the numerical range formed by any two of the above specific values ​​as endpoints.

[0033] Preferably, the substrate nutrient soil contains 3-15 parts by weight of yeast metabolites. For example, in some specific embodiments, the yeast metabolites may contain 3 parts, 3.2 parts, 3.4 parts, 3.6 parts, 3.8 parts, 4 parts, 4.2 parts, 4.4 parts, 4.6 parts, 4.8 parts, 5 parts, 5.2 parts, 5.4 parts, 5.6 parts, 5.8 parts, 6 parts, 6.2 parts, 6.4 parts, 6.6 parts, 6.8 parts, 7 parts, 7.2 parts, 7.4 parts, 7.6 parts, 7.8 parts, 8 parts, 8.2 parts, 8.4 parts, 8.6 parts, 8.8 parts, 9 parts, 9.2 parts, 9 ... 4, 9.6, 9.8, 10, 10.2, 10.4, 10.6, 10.8, 11, 11.2, 11.4, 11.6, 11.8, 12, 12.2, 12.4, 12.6, 12.8, 13, 13.2, 13.4, 13.6, 13.8, 14, 14.2, 14.4, 14.6, 14.8, or 15 parts of yeast metabolites, or yeast metabolites containing values ​​within the range defined by any two of the above specific values ​​as endpoints.

[0034] Preferably, the substrate nutrient soil contains 10-30 parts by weight of yeast residue. For example, in some specific embodiments, the yeast residue may contain 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 parts of yeast residue, or yeast residue within the numerical range formed by any two of the above specific values ​​as endpoints.

[0035] Preferably, the substrate nutrient soil contains 1-10 parts by weight of sugar residue. For example, in some specific embodiments, the sugar residue may contain 1 part, 1.2 parts, 1.4 parts, 1.6 parts, 1.8 parts, 2 parts, 2.2 parts, 2.4 parts, 2.6 parts, 2.8 parts, 3 parts, 3.2 parts, 3.4 parts, 3.6 parts, 3.8 parts, 4 parts, 4.2 parts, 4.4 parts, 4.6 parts, 4.8 parts, 5 parts, or 5.2 parts. Sugar residue of 5.4, 5.6, 5.8, 6, 6.2, 6.4, 6.6, 6.8, 7, 7.2, 7.4, 7.6, 7.8, 8, 8.2, 8.4, 8.6, 8.8, 9, 9.2, 9.4, 9.6, 9.8 or 10 parts, or sugar residue containing sugar residue within the numerical range formed by any two of the above specific values ​​as endpoints.

[0036] Preferably, the substrate nutrient soil contains 0.04-0.09 parts by weight of raspberry ketone. For example, in some specific embodiments, the raspberry ketone may contain 0.04 parts, 0.05 parts, 0.06 parts, 0.07 parts, 0.08 parts or 0.09 parts of raspberry ketone, or contain raspberry ketone within the numerical range formed by any two of the above specific values ​​as endpoints.

[0037] The following describes the sugarcane leaf powder, vermiculite, compound microbial agent, yeast metabolites, yeast residue, and sugar residue in the substrate nutrient soil of the present invention: The composite microbial agent used in this invention involves a combination of Bacillus subtilis, Bacillus licheniformis, Saccharomyces cerevisiae, Aspergillus oryzae, and lactic acid bacteria. The composite microbial agent contains 15-35 billion live Bacillus subtilis per gram of the composite microbial agent, preferably 20 billion.

[0038] Yeast metabolites refer to products refined through processes such as concentration, drying, or non-drying, which are produced by fermenting yeast metabolites after culturing yeast with molasses.

[0039] It should be noted that the yeast metabolites used in this invention are in liquid form. The following use of "by weight of yeast metabolites" refers to the direct use of the yeast metabolites for detecting the content of corresponding substances. The following use of "by dry weight of yeast metabolites" refers to drying the yeast metabolites using a drying process to achieve a moisture content of 48-52 wt%, followed by using the dried yeast metabolites for detecting the content of corresponding substances.

[0040] It should be noted that the drying process of the present invention is not particularly limited. Its main purpose is to ensure that the yeast metabolites achieve the required moisture content after drying and to ensure that the content of substances therein remains within the range specified in the present invention. Preferably, the drying process is vacuum freeze drying.

[0041] The yeast metabolites include both organic and inorganic substances. The organic substances are one or more of the following: amino acids, humic acid, lactic acid, succinic acid, citric acid, malic acid, acetic acid, dextran, mannan, trehalose, humic acid, betaine, auxin, cytokinin, abscisic acid, gibberellic acid, methyl salicylate, methyl jasmonic acid, vitamin C, and γ-aminobutyric acid.

[0042] Preferably, based on the weight of yeast metabolites, it contains 1-8 wt% amino acids, and / or 30-47 wt% fulvic acid, and / or 1-2.5 wt% lactic acid, and / or 0.2-0.9 wt% succinic acid, and / or 0.1-0.7 wt% citric acid, and / or 1-4 wt% malic acid, and / or 0.1-1.8 wt% acetic acid, and / or 0.04-0.09 wt% γ-aminobutyric acid.

[0043] Preferably, the yeast contains 150-280 mg of vitamin C per kg of yeast metabolites.

[0044] Preferably, based on the dry weight of yeast metabolites, it contains 1-2 wt% dextran, and / or 1-2 wt% mannan, and / or 6-8 wt% trehalose, and / or 3-9 wt% humic acid, and / or 5-12 wt% betaine.

[0045] Preferably, per gram of yeast metabolite, it contains 4-8 ng of auxin, and / or 0.9-4.1 ng of cytokinin, and / or 2.6-5.4 ng of abscisic acid, and / or 0.5-3.1 ng of gibberellic acid, and / or 28-56 ng of methyl salicylate, and / or 1-3 ng of methyl jasmonic acid.

[0046] The inorganic substance is one or more of nitrogen, phosphorus, potassium, calcium, magnesium, sulfur, iron, manganese, copper, boron, molybdenum and zinc; preferably, the inorganic substance is one or more of nitrogen, phosphorus, potassium, calcium, magnesium, sulfur, iron, manganese, copper and zinc.

[0047] Preferably, based on the dry weight of the yeast metabolites, the yeast metabolites contain 4.5-5 wt% nitrogen, and / or 0.5-0.55 wt% phosphorus, and / or 12-13 wt% potassium, and / or 0.0150-0.0170 wt% calcium, and / or 0.0100-0.0105 wt% magnesium, and / or 2.5-3 wt% sulfur, and / or 0.000484-0.0004845 wt% iron, and / or 0.0001075-0.0001079 wt% manganese, and / or 0.00000601-0.00000604 wt% copper, and / or 0.000350-0.000355 wt% zinc.

[0048] Yeast residue refers to byproducts generated during the biochemical and physicochemical stages of yeast fermentation, including incompletely utilized yeast cells, cell fragments, metabolic products, and residues mixed with other ingredients. Preferably, the yeast residue raw material used in this invention is derived from baker's yeast, primarily high-sugar baker's yeast and sugar-free baker's yeast.

[0049] Sugar residue refers to the waste generated during the sugar-making process, mainly composed of the residues of sugar-making raw materials. After sugar extraction, these residues leave behind a large amount of cellulose and other organic matter. As an organic waste, sugar residue is rich in organic matter, nitrogen, phosphorus, potassium, and other nutrients. Sugar residue is mainly divided into starch residue, beet residue, sugarcane bagasse, and fruit pomace. The sugar residue used in the embodiments and comparative examples of this invention is sugarcane bagasse.

[0050] The roles of sugarcane leaf powder, vermiculite, compound microbial agents, yeast metabolites, yeast residue, and sugar residue in the substrate nutrient soil are as follows: The role of sugarcane leaf powder in substrate nutrient soil is as follows: After fermentation, sugarcane leaf powder is permeable to water and air, making the structure of the substrate nutrient soil more porous, preventing plants from dying due to root hypoxia. It also has good water retention capacity, increases the looseness of the pile during fermentation, and promotes fermentation efficiency. At the same time, it is rich in organic matter and trace elements, providing the necessary nutrients for cultivated crops and improving the quality of cultivated crops.

[0051] The role of vermiculite in substrate nutrient soil is to regulate the moisture content of the substrate nutrient soil and promote water and fertilizer retention.

[0052] The role of compound microbial agents in substrate nutrient soil is to promote decomposition and fermentation, and increase the degree of decomposition. During fermentation, they can also utilize the organic matter in the soil to produce secondary metabolites, which contain a large number of growth-promoting substances, such as auxins, that can promote plant elongation.

[0053] The role of yeast metabolites in substrate nutrient soil is as follows: Yeast metabolites can act as biostimulants, improving soil structure, promoting growth, enhancing stress resistance, improving quality, and increasing yield. Specifically, beneficial yeast bacteria multiply rapidly around crop roots, and the colloidal substances they secrete promote the formation of soil aggregates, making the soil loose, aerated, and able to retain water and fertilizer. Furthermore, yeast can effectively decompose substances such as ammonia, phosphorus, and potassium fixed in the soil, converting them into nutrients that can be directly absorbed and utilized by crops. Simultaneously, it provides ample carbon and nitrogen sources and growth factors for microbial growth, effectively inhibiting the growth of harmful bacteria, viruses, and pests, and improving the root growth environment of cultivated crops.

[0054] The role of yeast residue in substrate nutrient soil is as follows: it contains a large amount of nutrients such as protein, amino acids, and vitamins, providing abundant nutrients for plants and promoting their growth and development. At the same time, the high water content of yeast residue has excellent water and fertilizer retention properties, which can improve soil structure and enhance soil fertility.

[0055] The role of sugar residue in substrate nutrient soil is that the nutrients such as nitrogen, phosphorus, and potassium in sugar residue can be absorbed and utilized by cultivated crops, promoting crop growth and development, and improving yield and quality.

[0056] The role of raspberry ketone in substrate nutrient soil is as a fragrance agent, which can enhance the sensory aroma of the substrate nutrient soil.

[0057] The substances in the substrate nutrient soil of the present invention have been described above. However, sugarcane leaf powder, vermiculite, compound microbial agents, yeast metabolites, yeast residue, and sugar residue obtained by conventional means or commercially available to those skilled in the art can all be used in the substrate nutrient soil of the present invention. The above description of the acquisition methods or sources of the sugarcane leaf powder, vermiculite, compound microbial agents, yeast metabolites, yeast residue, and sugar residue used in the present invention is merely an example. In fact, the sugarcane leaf powder, vermiculite, compound microbial agents, yeast metabolites, yeast residue, and sugar residue used in the present invention are not limited to the above acquisition methods or sources, and may also use other substances obtained by conventional means by those skilled in the art; or use the above substances commercially available on the market.

[0058] The method for preparing the substrate nutrient soil provided in this embodiment of the invention specifically includes the following steps: (1) Mixing: Sugar residue, yeast residue, sugarcane leaf powder and compound microbial agent are mixed to obtain a mixture; (2) Stacking: The mixture obtained in step (1) is stacked into a large pile with a height of 1-1.5m (based on the highest height of the large pile). (3) Turning: When the temperature of the mixture obtained in step (2) reaches 60-80℃, the first turning is carried out, and then the second turning is carried out when the temperature reaches 60-80℃. The average time interval between turning is 5-7 days / time. (4) Fermentation: The mixture after the second turning in step (3) is placed naturally for fermentation. The fermentation temperature is kept below 40℃, preferably at 35-40℃ to complete the cycle of fermentation. The natural placement time is 5-10 days.

[0059] (5) Crushing: The mixture obtained in step (4) is crushed and then sieved; (6) Mixing: The mixture obtained in step (5) is mixed with vermiculite and raspberry ketone to obtain substrate nutrient soil; (7) Packaging: Pack and store the substrate nutrient soil.

[0060] The product packaging preferably consists of an inner plastic bag and an outer woven bag. Storage conditions are as follows: store in a cool, dry place at room temperature, avoiding direct sunlight.

[0061] To better understand the technical solution of the present invention, the technical solution of the present invention will be described in detail below with reference to specific embodiments.

[0062] Unless otherwise stated, all reagents / instruments used in the embodiments and comparative examples of this invention are conventional commercially available products. Information on the experimental materials and instruments used in this invention is shown in the table below: The specific components of the yeast metabolites used in the examples and comparative examples are as follows: It should be noted that the yeast metabolites used in this invention are in liquid form. The following descriptions of yeast metabolites by weight refer to the direct use of the yeast metabolites for determining the content of corresponding substances. The following descriptions of yeast metabolites by dry weight refer to the use of the dried yeast metabolites, where the moisture content is 48-52 wt%, for determining the content of corresponding substances. The yeast metabolites used in the examples and comparative examples include organic and inorganic substances.

[0063] The organic substances are amino acids, fulvic acid, lactic acid, succinic acid, citric acid, malic acid, acetic acid, dextran, mannan, trehalose, humic acid, betaine, auxin, cytokinin, abscisic acid, gibberellic acid, methyl salicylate, methyl jasmonic acid, vitamin C, and γ-aminobutyric acid. The inorganic substances are nitrogen, phosphorus, potassium, calcium, magnesium, sulfur, iron, manganese, copper, and zinc.

[0064] Based on the weight of yeast metabolites, it contains 4.38 wt% amino acids, 46.4 wt% fulvic acid, 2.03 wt% lactic acid, 0.43 wt% succinic acid, 0.65 wt% citric acid, 1.82 wt% malic acid, 0.84 wt% acetic acid, and 0.07 wt% γ-aminobutyric acid.

[0065] Based on the weight of yeast metabolites, it contains 166 mg of vitamin C.

[0066] Based on the dry weight of yeast metabolites, it contains 1-2 wt% dextran, 1-2 wt% mannan, 6-8 wt% trehalose, 4.01 wt% humic acid, and 8 wt% betaine.

[0067] Based on the dry weight of each gram of yeast metabolites, it contains 5.51 ng of auxin, 1.40 ng of cytokinin, 3.702 ng of abscisic acid, 1.11 ng of gibberellic acid, 39.6 ng of methyl salicylate, and 2.13 ng of methyl jasmonic acid.

[0068] Based on the dry weight of yeast metabolites, yeast metabolites contain 4.83 wt% nitrogen, 0.52 wt% phosphorus, 12.72 wt% potassium, 0.0161 wt% calcium, 0.0102 wt% magnesium, 2.86 wt% sulfur, 0.0004842 wt% iron, 0.0001078 wt% manganese, 0.00000604 wt% copper and 0.000352 wt% zinc.

[0069] Example 1 The substrate nutrient soil provided in Example 1 comprises: 17g sugarcane leaf powder, 44.9g vermiculite, 0.03g compound microbial agent, 3g yeast metabolites, 30g yeast residue, 5g sugarcane bagasse, and 0.07g raspberry ketone.

[0070] The method for preparing the substrate nutrient soil provided in Example 1 specifically includes the following steps: (1) Mixing: Sugarcane leaf powder, compound microbial agent, yeast metabolites, yeast residue and sugarcane bagasse are mixed to obtain a mixture; (2) Stacking: The mixture obtained in step (1) is stacked into a large pile with a height of 1.5m (based on the highest height of the large pile). (3) Turning: When the mixture obtained in step (2) reaches 60°C, the first turning is carried out, and then the second turning is carried out when the temperature reaches 60°C. The average time interval between turning is 5-7 days / time. (4) Fermentation: After the mixture in step (3) is turned over for the second time, let it sit naturally for 6 days. When the fermentation temperature is stable at 35℃, the cycle fermentation is completed. (5) Crushing: The mixture obtained in step (4) after the completion of the fermentation cycle is crushed and passed through a 6-mesh standard sieve to obtain the target mixture. (6) Mixing: The target mixture obtained in step (5) is mixed with vermiculite and raspberry ketone to obtain substrate nutrient soil; (7) Packaging: The substrate nutrient soil is packaged and stored. The product packaging consists of an inner plastic bag and an outer woven bag. The storage conditions are: store in a cool, dry place at room temperature, avoiding direct sunlight.

[0071] Example 2 The substrate nutrient soil provided in Example 2 comprises: 23g sugarcane leaf powder, 43g vermiculite, 0.05g compound microbial agent, 6g yeast metabolites, 22g yeast residue, 5.9g sugarcane bagasse, and 0.05g raspberry ketone.

[0072] The preparation method of the substrate nutrient soil provided in Example 2 is the same as that in Example 1.

[0073] Example 3 The substrate nutrient soil provided in Example 3 comprises: 27g sugarcane leaf powder, 38g vermiculite, 0.06g compound microbial agent, 6.3g yeast metabolites, 19g yeast residue, 9.6g sugarcane bagasse, and 0.04g raspberry ketone.

[0074] The preparation method of the substrate nutrient soil provided in Example 3 is the same as that in Example 1.

[0075] Example 4 The substrate nutrient soil provided in Example 4 comprises: 24g sugarcane leaf powder, 41g vermiculite, 0.01g compound microbial agent, 4.94g yeast metabolites, 23g yeast residue, 7.0g sugarcane bagasse, and 0.05g raspberry ketone.

[0076] The preparation method of the substrate nutrient soil provided in Example 4 is the same as that in Example 1.

[0077] Example 5 The substrate nutrient soil provided in Example 5 comprises: 18g sugarcane leaf powder, 40.4g vermiculite, 0.04g compound microbial agent, 15g yeast metabolites, 21g yeast residue, 5.5g sugarcane bagasse, and 0.06g raspberry ketone.

[0078] The preparation method of the substrate nutrient soil provided in Example 5 is the same as that in Example 1.

[0079] Example 6 The substrate nutrient soil provided in Example 6 comprises: 26.4g sugarcane leaf powder, 48g vermiculite, 0.05g compound microbial agent, 6g yeast metabolites, 10g yeast residue, 9.5g sugarcane bagasse, and 0.05g raspberry ketone.

[0080] The preparation method of the substrate nutrient soil provided in Example 6 is the same as that in Example 1.

[0081] Comparative Example 1 The substrate nutrient soil provided in Comparative Example 1 includes 100g of vermiculite.

[0082] Comparative Example 2 The substrate nutrient soil provided in Comparative Example 2 comprises: 11g sugarcane leaf powder, 49.89g vermiculite, 0.03g compound microbial agent, 3g yeast metabolites, 32g yeast residue, 4g sugarcane bagasse, and 0.08g raspberry ketone.

[0083] The preparation method of the substrate nutrient soil provided in Comparative Example 2 is the same as that in Example 1.

[0084] Comparative Example 3 The substrate nutrient soil provided in Comparative Example 3 comprises: 15g sugarcane leaf powder, 28g vermiculite, 0.03g compound microbial agent, 8.9g yeast metabolites, 45g yeast residue, 3g sugarcane bagasse, and 0.07g raspberry ketone. The preparation method of the substrate nutrient soil provided in Comparative Example 3 is the same as that in Example 1.

[0085] Comparative Example 4 The substrate nutrient soil provided in Comparative Example 4 comprises: 34g sugarcane leaf powder, 18g vermiculite, 0.03g compound microbial agent, 2.87g yeast metabolites, 35g yeast residue, 10g sugarcane bagasse, and 0.1g raspberry ketone. The preparation method of the substrate nutrient soil provided in Comparative Example 4 is the same as that in Example 1.

[0086] It should be noted that, through statistical analysis and comparison of the effects of the substrate nutrient soil obtained in Examples 1-6 and Comparative Examples 1-4 on the germination rate of radishes, tomatoes, chili peppers, eggplants, and cucumbers, as well as on the leaf quantity and plant height of wheat and water spinach, it can be seen that the crops cultivated in the substrate nutrient soil obtained in Examples 1-6 are superior to those in Comparative Examples 1-4 in terms of germination rate, leaf quantity, and plant height. Meanwhile, statistical analysis and comparison of the effects of the substrate nutrient soil obtained in Example 1 on the germination rate of radishes, tomatoes, chili peppers, eggplants, and cucumbers, as well as on the leaf quantity and plant height of wheat and water spinach, shows that the crops cultivated in the substrate nutrient soil of Example 1 are slightly superior to those in other examples in terms of germination rate, leaf quantity, and plant height.

[0087] Therefore, the following data on the effects of the substrate nutrient soil obtained in Example 1 and some comparative examples on the germination rate of radishes, tomatoes, chili peppers, eggplants and cucumbers, as well as the effects on the number of leaves and plant height of wheat and water spinach, are used to evaluate the effects of the substrate nutrient soil provided by the present invention on radishes, tomatoes, chili peppers, eggplants, cucumbers, wheat and water spinach, as shown in Application Examples 1, 2 and 3 below.

[0088] Application Example 1: Application of substrate nutrient soil in seed germination Radishes, tomatoes, chili peppers, eggplants, and cucumbers were used as experimental crops and cultivated using inclined seedling trays. Details are as follows: (1) The substrate nutrient soil of Examples 1, 2, 3 and 4 was filled into seedling trays for cultivation of the above 5 experimental crops. Based on the same substrate nutrient soil, four parallel groups were set up for each experimental crop. Each parallel group was sown with 5 seeds (the number of seeds for each crop was the same). The germination rate of radish, tomato, chili pepper, eggplant and cucumber was recorded. During the entire experiment, no fertilizer was provided, and the substrate nutrient soil was not replenished or replaced. Only water was added.

[0089] (2) On days 7, 14, 21, and 28, the germination rates of the substrate nutrient soils used in Examples 1, 2, 3, and 4 for radishes, tomatoes, chili peppers, eggplants, and cucumbers were observed. The results are as follows: Figure 1 As shown. Figure 1 The germination rate of the substrate nutrient soil of Example 1 on radishes, tomatoes, chili peppers, eggplants and cucumbers was observed on days 7, 14, 21 and 28. Figure 1 (a) in the figure represents the germination rate of radish, tomato, chili pepper, eggplant and cucumber in the substrate nutrient soil of Example 1 on day 7; Figure 1 (b) in the figure represents the germination rate of radish, tomato, chili pepper, eggplant and cucumber in the substrate nutrient soil of Example 1 on day 14; Figure 1 (c) in the figure represents the germination rate of radish, tomato, chili pepper, eggplant and cucumber in the substrate nutrient soil of Example 1 on day 21; Figure 1 (d) in the figure represents the germination rate of radish, tomato, chili pepper, eggplant, and cucumber in the substrate nutrient soil of Example 1 on day 28; where, in Figure 1 (a) Figure 1 (b) Figure 1 (c) and Figure 1 In (d), the experimental crops cultivated from near to far are: cucumber, eggplant, chili pepper, tomato, and radish. Each row contains four seed trays containing the same experimental crop cultivated in the substrate nutrient soil of Example 1, i.e., four parallel groups are set up for each experimental crop.

[0090] (3) On days 7, 14, 21, and 28, the germination rates of the substrate nutrient soils used in Examples 1, 2, 3, and 4 for radishes, tomatoes, chili peppers, eggplants, and cucumbers were statistically analyzed. Specific data statistics are as follows: Figure 2 As shown. Figure 2(a) in the figure represents the statistical results of the germination rate of radish, tomato, chili pepper, eggplant and cucumber on day 7 using the substrate nutrient soil of Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4. In the figure, the strips corresponding to radish, tomato and cucumber are Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4 from left to right, the strips corresponding to chili pepper are Example 1, Comparative Example 2 and Comparative Example 4 from left to right, and the strips corresponding to eggplant are Example 1 and Comparative Example 2 from left to right. Figure 2 (b) in the figure represents the statistical results of the germination rate of radish, tomato, chili pepper, eggplant and cucumber on day 7 using the substrate nutrient soil of Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4. In the figure, the strips corresponding to radish, tomato, chili pepper and cucumber are Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4 from left to right, and the strips corresponding to eggplant are Example 1, Comparative Example 2 and Comparative Example 4 from left to right. Figure 2 (c) in the figure represents the statistical results of the germination rate of radish, tomato, chili pepper, eggplant and cucumber by the substrate nutrient soil of Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4 on day 21. In the figure, the strips corresponding to radish, tomato, chili pepper and cucumber are Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4 from left to right, and the strips corresponding to eggplant are Example 1, Comparative Example 2 and Comparative Example 4 from left to right. Figure 2 In the figure, (d) represents the statistical results of the germination rate of radish, tomato, chili pepper, eggplant, and cucumber on day 28 using the substrate nutrient soils of Examples 1, 2, 3, and 4. In the figure, the strips corresponding to radish, from left to right, represent Examples 1, 2, and 3; the strips corresponding to tomato, chili pepper, and eggplant, from left to right, represent Examples 1, 2, 3, and 4; and the strips corresponding to cucumber, from left to right, represent Examples 1, 2, and 4. The statistical results are the average of the germination rate data from the four parallel groups. like Figure 1 and Figure 2As shown, the results indicate that the substrate nutrient soil of Example 1 can be used for seedling cultivation and can directly contact the seeds. Overall, in terms of germination rate, plant height, and crop fresh weight, the substrate nutrient soil of Example 1 is superior to that of Comparative Example 1 in terms of germination rate and plant height of radishes, tomatoes, chili peppers, eggplants, and cucumbers, mainly reflected in growth and the absence of targeted nutrient deficiencies. The substrate nutrient soil of Example 1 resulted in more consistent growth of radishes, tomatoes, chili peppers, eggplants, and cucumbers, with thicker stems. Furthermore, the leaves of radishes, tomatoes, chili peppers, eggplants, and cucumbers cultivated with the substrate nutrient soil of Example 1 were normal, and nutrient deficiency was not obvious. However, the radishes, tomatoes, chili peppers, eggplants, and cucumbers cultivated with the substrate nutrient soils of Comparative Examples 2, 3, and 4 showed more severe nutrient deficiencies. Among them, the radishes, tomatoes, chili peppers, eggplants, and cucumbers cultivated with the substrate nutrient soil of Comparative Example 1 showed the most severe nutrient deficiencies, specifically manifested as yellowing leaves, which was judged as a nutrient deficiency symptom.

[0091] Application Example 2: Application of substrate nutrient soil in wheat cultivation Wheat was used as the experimental crop, and it was cultivated using seedling trays. Details are as follows: (1) The substrate nutrient soils of Example 1, Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4 were filled into seedling trays for wheat experimental crop cultivation. Based on the same substrate nutrient soil, three parallel groups were set up for each experimental crop. Four seedlings were sown in each parallel group, and the number of wheat leaves and plant height were recorded. Throughout the experiment, no fertilizer was provided, and the substrate nutrient soil was not replenished or replaced; only water was provided.

[0092] (2) On days 7 and 28, the effects of the substrate nutrient soils of Examples 1, 1, 2, 3, and 4 on the number of wheat leaves and plant height were observed. Simultaneously, the nutrient deficiency of the wheat was observed, determined by leaf growth vigor, yellowing, and growth level. Specific results are as follows: Figure 3 As shown.

[0093] Figure 3 (a) in the figure represents the growth of wheat on day 7 using the substrate nutrient soils of Examples 1, 1, 2, 3, and 4. Figure 3 (b) in the figure represents the growth of wheat on day 28 using the substrate nutrient soils of Examples 1, 1, 2, 3, and 4. Figure 3 (a) and Figure 3In (b), from left to right, the growth of wheat in the substrate nutrient soil of Comparative Example 1, Comparative Example 2, Example 1, Comparative Example 3 and Comparative Example 4 are shown. Each column contains 3 cultivation pots of wheat cultivated in the same substrate nutrient soil, i.e., 3 parallel groups are set up.

[0094] (3) On day 28, the number of wheat leaves and plant height were recorded for the effects of the substrate nutrient soils of Example 1, Comparative Example 1, Comparative Example 2, Comparative Example 3, and Comparative Example 4. Specific results are as follows: Figure 4 As shown.

[0095] Figure 4 (a) in the figure represents the statistical results of the number of wheat leaves on the substrate nutrient soil of Example 1, Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4 on day 28. Figure 4 (b) in the table represents the statistical results of the effect of the substrate nutrient soil of Examples 1, 1, 2, 3, and 4 on the plant height of wheat on day 28. The statistical results are the average of the number of leaves and plant height data of the three parallel groups.

[0096] like Figure 3 and Figure 4 As shown, the results indicate that, in terms of overall plant growth, the wheat cultivated in the substrate nutrient soil of Example 1 had the best growth, being tender, green, and robust. However, the wheat cultivated in the substrate nutrient soil of Comparative Examples 2, 3, and 4 showed obvious symptoms of nutrient deficiency, poor growth, and slight yellowing. The wheat cultivated using the substrate nutrient soil of Comparative Example 1 had withered and yellow leaves, indicating root burn, and one seedling died in each of the three parallel groups.

[0097] Application Example 3: Application of substrate nutrient soil in the cultivation of water spinach Water spinach was used as the experimental crop, and it was cultivated using seedling trays. Details are as follows: (1) The substrate nutrient soil of Example 1, Comparative Example 3 and Comparative Example 4 were filled into seedling trays to cultivate water spinach experimental crops. Based on the same substrate nutrient soil, three parallel groups were set up for each experimental crop. Four seedlings were sown in each parallel group, and the number of leaves and plant height of water spinach were recorded. No nutrients were given in the later stage of the experiment, only water was added.

[0098] (2) On days 7 and 28, the effects of the substrate nutrient soils of Example 1, Comparative Example 3, and Comparative Example 4 on the number of leaves and plant height of water spinach were observed. Simultaneously, the nutrient deficiency of the water spinach was observed, and the nutrient deficiency was determined by the leaf growth vigor, whether it turned yellow, and the degree of growth. Specific results are as follows: Figure 5 As shown.

[0099] Figure 5 (a) in the figure represents the growth of water spinach on day 7 using the substrate nutrient soil of Example 1, Comparative Example 3, and Comparative Example 4. Figure 5 (b) in the figure represents the growth of water spinach on day 28 using the substrate nutrient soils of Example 1, Comparative Example 3, and Comparative Example 4. Among them, in Figure 5 (a) and Figure 5 In (b), from left to right, the growth of wheat in the substrate nutrient soil of Example 1, Comparative Example 3 and Comparative Example 4 are shown. Each column contains 3 cultivation pots with the same substrate nutrient soil, that is, 3 parallel groups are set up.

[0100] (3) On day 28, the effects of the substrate nutrient soil of Example 1, Comparative Example 3, and Comparative Example 4 on the number of leaves and plant height of water spinach were recorded. Specific results are as follows: Figure 6 As shown.

[0101] Figure 6 (a) in the figure represents the statistical results of the effect of the substrate nutrient soil of Example 1, Comparative Example 3 and Comparative Example 4 on the number of leaves of water spinach on day 28. Figure 6 (b) in the table represents the statistical results of the effect of the substrate nutrient soil of Example 1, Comparative Example 3, and Comparative Example 4 on the plant height of water spinach on day 28. The statistical results are the average of the number of leaves and plant height data of the three parallel groups.

[0102] like Figure 5 and Figure 6 As shown, the results indicate that, in terms of overall plant growth, the water spinach grown in the substrate nutrient soil of Example 1 has the best growth, being tender, green, and robust; however, the water spinach grown in the substrate nutrient soil of Comparative Example 1 shows obvious symptoms of nutrient deficiency, poor growth, small and dull leaves, and some yellowing.

[0103] While the specific embodiments of the present invention have been described above, they are not intended to limit the scope of protection of the present invention. Based on the technical solutions of the present invention, various modifications or variations that can be made by those skilled in the art without creative effort are still within the scope of protection of the present invention.

Claims

1. A substrate nutrient soil for use in the seedling stage, characterized in that, By weight, the substrate nutrient soil comprises: 17-27 parts sugarcane leaf powder, 30-55 parts vermiculite, 0.03-0.06 parts compound microbial agent, 3-7 parts yeast metabolites, 19-30 parts yeast residue, 5-9.6 parts sugarcane bagasse, and 0.04-0.09 parts raspberry ketone. The compound microbial agent is composed of Bacillus subtilis, Bacillus licheniformis, Saccharomyces cerevisiae, Aspergillus oryzae, and lactic acid bacteria. The number of viable Bacillus subtilis in each g of compound microbial agent is 150-350 billion. Based on the weight of yeast metabolites, it contains 1-8 wt% amino acids, and / or 30-47 wt% fulvic acid, and / or 1-2.5 wt% lactic acid, and / or 0.2-0.9 wt% succinic acid, and / or 0.1-0.7 wt% citric acid, and / or 1-4 wt% malic acid, and / or 0.1-1.8 wt% acetic acid, and / or 0.04-0.09 wt% γ-aminobutyric acid.

2. The substrate nutrient soil according to claim 1, characterized in that, By weight, the substrate nutrient soil comprises: 38-45 parts vermiculite and / or 0.04-0.07 parts raspberry ketone.

3. A method for preparing the substrate nutrient soil according to claim 1 or 2, characterized in that, The preparation method includes the following steps: (1) Mixing: Sugar residue, yeast residue, yeast metabolites, sugarcane leaf powder and compound microbial agent are mixed to obtain a mixture; (2) Stacking: Stack the mixture obtained in step (1); (3) Turning over: The mixture obtained in step (2) is turned over; (4) Fermentation: Ferment the mixture after turning over in step (3); (5) Crushing: The fermented mixture obtained in step (4) is crushed to obtain the target mixture; (6) Mixing: The target mixture obtained in step (5) is mixed with vermiculite and raspberry ketone to obtain substrate nutrient soil.

4. The preparation method according to claim 3, characterized in that, In step (3), turning over: when the temperature of the mixed material after stacking obtained in step (2) reaches 60-80℃, turning over is carried out.

5. The preparation method according to claim 3 or 4, characterized in that, In step (4), the mixture after the second turning in step (3) is placed naturally for fermentation, and the fermentation temperature is stabilized below 40℃, preferably at 35-40℃ to complete the cycle fermentation.

6. The preparation method according to any one of claims 3-5, characterized in that, The natural placement time is 5-10 days.

7. The application of the substrate nutrient soil according to claim 1 or 2 or the substrate nutrient soil prepared by any one of claims 3-6 in the cultivation of Cucurbitaceae plants, preferably in the cultivation of cucumbers.

8. The application of the substrate nutrient soil according to claim 1 or 2 or the substrate nutrient soil prepared by any one of claims 3-6 in the cultivation of Solanaceae plants, preferably in the cultivation of eggplant, chili pepper and tomato or more.

9. The application of the substrate nutrient soil according to claim 1 or 2 or the substrate nutrient soil prepared by any one of claims 3-6 in the cultivation of cruciferous plants, preferably in the cultivation of radishes.

10. The application of the substrate nutrient soil according to claim 1 or 2 or the substrate nutrient soil prepared by any one of claims 3-6 in the cultivation of gramineous plants, preferably in the cultivation of wheat.

11. The application of the substrate nutrient soil according to claim 1 or 2 or the substrate nutrient soil prepared by the preparation method according to any one of claims 3-6 in the cultivation of Convolvulaceae plants, preferably in the cultivation of water spinach.

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