Composition for improving stability of N-acetylneuraminic acid and application thereof

Abstract

The invention relates to the technical field of cosmetics, in particular to a composition for improving the stability of N-acetylneuraminic acid and application of the composition. The composition further comprises ergothioneine and / or hydroxyethyl piperazine ethanesulfonic acid; when the creatine is used for improving the stability of the N-acetylneuraminic acid, the ratio of the creatine to the N-acetylneuraminic acid is (0.01-1.5): 0.2; preferably, the ratio of the creatine to the N-acetylneuraminic acid is (0.2-1.5): 0.2. The invention also provides an application of creatine in improving the stability of N-acetylneuraminic acid, and further provides an application of creatine as a unique stabilizer in improving the stability of N-acetylneuraminic acid.

Description

Technical Field

[0001] This application relates to the field of cosmetic technology, specifically to a composition for improving the stability of N-acetylneuraminic acid and its application. Background Technology

[0002] N-Acetylneuraminic Acid (NANA), also known as sialic acid, is an acidic nonose sugar widely found in organisms, named after its initial extraction from bird's nest. In the cosmetics industry, sialic acid has garnered significant attention due to its unique bioactivity. It enhances the moisturizing ability of the stratum corneum by forming a hydration film with water molecules, reducing moisture loss and keeping the skin hydrated. Simultaneously, sialic acid possesses antioxidant properties, scavenging free radicals, inhibiting oxidative stress damage to skin cells, and delaying skin aging. Furthermore, it regulates skin cell metabolism, helping to promote normal cell turnover in the stratum corneum, resulting in smooth and delicate skin.

[0003] However, sialic acid faces significant challenges in its practical application in cosmetics, with insufficient stability being a particularly prominent issue. The sialic acid molecule contains multiple hydroxyl and carboxyl groups, which make it susceptible to hydrolysis under high temperature and humidity conditions, leading to molecular structure destruction and reduced activity. When coexisting with common cosmetic ingredients such as certain preservatives and pH adjusters, chemical reactions may occur, further affecting its stability. Under light exposure, sialic acid may also undergo photodegradation, resulting in a decrease in its effective content. Poor stability not only weakens the efficacy of sialic acid in cosmetics but may also lead to unstable product quality, causing phenomena such as layering and discoloration, affecting consumer experience and product market competitiveness. Therefore, how to effectively improve the stability of sialic acid in cosmetics and fully utilize its bioactive functions has become a key technical problem urgently needing to be solved in the cosmetics industry. Summary of the Invention

[0004] To address the aforementioned problems, this application provides a composition for improving the stability of N-acetylneuraminic acid and its application.

[0005] The technical solution of this application is as follows:

[0006] 1. A composition for improving the stability of N-acetylneuraminic acid, comprising:

[0007] Creatine.

[0008] 2. The composition according to claim 1, further comprising:

[0009] Ergothioneine, and / or

[0010] Hydroxyethylpiperazine ethane sulfonic acid.

[0011] 3. The composition according to claim 1, wherein, in order to improve the stability of N-acetylneuraminic acid, the ratio of creatine to N-acetylneuraminic acid is (0.01 to 1.5):0.2; preferably, the ratio of creatine to N-acetylneuraminic acid is (0.2 to 1.5):0.2.

[0012] 4. The composition according to claim 2, wherein, in order to improve the stability of N-acetylneuraminic acid, the ratio of N-acetylneuraminic acid to ergothioneine and / or hydroxyethylpiperazine ethanesulfonic acid is 0.2:(0.01-2) and / or 0.2:(0.01-8); preferably, the ratio of N-acetylneuraminic acid to ergothioneine and / or hydroxyethylpiperazine ethanesulfonic acid is 0.2:(0.2-2) and / or 0.2:(0.2-8).

[0013] 5. Use of creatine to improve the stability of N-acetylneuraminic acid.

[0014] 6. Use of creatine, ergothioneine, and / or hydroxyethylpiperazine ethanesulfonic acid in combination to improve the stability of N-acetylneuraminic acid.

[0015] 7. A composition comprising:

[0016] N-acetylneuraminic acid, and

[0017] Creatine.

[0018] 8. A composition comprising: N-acetylneuraminic acid and creatine; wherein the composition comprises only creatine as a stabilizer for N-acetylneuraminic acid.

[0019] 9. The composition according to claim 7, further comprising:

[0020] Ergothioneine, and / or

[0021] Hydroxyethylpiperazine ethane sulfonic acid.

[0022] 10. The composition according to item 7 or 8, wherein the percentage content of each component in the composition satisfies the ratio of N-acetylneuraminic acid to creatine of 0.2:(0.01 to 1.5); preferably the ratio of N-acetylneuraminic acid to creatine of 0.2:(0.2 to 1.5).

[0023] 11. The composition according to item 7 or 9, wherein the percentage content of each component in the composition satisfies the ratio of N-acetylneuraminic acid to ergothioneine and / or hydroxyethylpiperazine ethanesulfonic acid as 0.2:(0.01-2) and / or 0.2:(0.01-8); preferably 0.2:(0.2-2) and / or 0.2:(0.2-8).

[0024] 12. The use of the composition for improving the stability of N-acetylneuraminic acid as described in any one of items 1 to 4, or the composition as described in any one of items 7 to 11, in cosmetics.

[0025] 13. A method for stabilizing N-acetylneuraminic acid, comprising: mixing N-acetylneuraminic acid with creatine.

[0026] 14. A method for stabilizing N-acetylneuraminic acid, comprising: mixing N-acetylneuraminic acid with creatine, and / or ergothioneine, and / or hydroxyethylpiperazine ethanesulfonic acid.

[0027] The beneficial effects of this application are as follows:

[0028] This application discloses the use of creatine to improve the stability of N-acetylneuraminic acid. When used alone at a low dose, creatine can maintain the stability of N-acetylneuraminic acid for 40 days without discoloration. Furthermore, creatine can be compounded with conventional compositions (such as ergothioneine and hydroxyethylpiperazine ethanesulfonic acid) to jointly improve the stability of N-acetylneuraminic acid. The applicant has also discovered that the amounts of hydroxyethylpiperazine ethanesulfonic acid and ergothioneine in a certain ratio exhibit antagonistic effects, which can actually decrease the stability of N-acetylneuraminic acid. The composition provided in this application can improve the stability of N-acetylneuraminic acid without requiring complex operating equipment, and the operation is simple. Attached Figure Description

[0029] The accompanying drawings are provided to better understand this application and do not constitute an undue limitation thereof. Wherein:

[0030] Figure 1 The photographs showing the degree of color change of the compositions in Examples 1-17 and Comparative Examples 1-7 are shown; wherein the numbers corresponding to the examples or comparative examples are marked in the corresponding containers; for example, "Example 1" represents the color change of the composition in Example 1, and "Comparative 1" represents the color change of the composition in Comparative Example 1. Detailed Implementation

[0031] The present application is further illustrated below with reference to embodiments. It should be understood that the embodiments are only used to further illustrate and explain the present application and are not intended to limit the present application.

[0032] This application discloses compositions and their applications, as well as cosmetics. Those skilled in the art can refer to the content herein to appropriately modify process parameters to achieve the desired results. It should be particularly noted that all similar substitutions and modifications are obvious to those skilled in the art and are considered to be included in this application. The methods and applications of this application have been described through preferred embodiments. Those skilled in the art can clearly modify or appropriately change and combine the methods and applications described herein without departing from the content, spirit, and scope of this application to realize and apply the technology of this application.

[0033] Unless otherwise defined, technical and scientific terms used in this specification have the same meaning as commonly understood by one of ordinary skill in the art. While similar or identical methods and materials may be applied in experimental or practical applications, materials and methods are described herein. In case of conflict, the definitions included herein shall prevail. Furthermore, materials, methods, and examples are for illustrative purposes only and are not intended to be limiting. The present application is further described below with reference to specific embodiments, but is not intended to limit the scope of the application.

[0034] In this application, "stabilizer" refers to a substance that can maintain the physical and chemical stability of a solution system and prevent it from undergoing adverse changes such as deterioration, decomposition, and precipitation; furthermore, this application mainly discusses stabilizers for improving N-acetylneuraminic acid.

[0035] This application provides a composition for improving the stability of N-acetylneuraminic acid, wherein creatine is included.

[0036] In one specific embodiment of this application, the composition for improving the stability of N-acetylneuraminic acid further includes ergothioneine.

[0037] In one specific embodiment of this application, the composition for improving the stability of N-acetylneuraminic acid further includes: hydroxyethylpiperazine ethanesulfonic acid.

[0038] In one specific embodiment of this application, the composition for improving the stability of N-acetylneuraminic acid further includes ergothioneine and hydroxyethylpiperazine ethanesulfonic acid.

[0039] This application provides the use of creatine to improve the stability of N-acetylneuraminic acid, and further provides the use of creatine as the sole stabilizer to improve the stability of N-acetylneuraminic acid.

[0040] In one specific embodiment of this application, the percentage content of each component in a composition for improving the stability of N-acetylneuraminic acid includes: 0.01-2% ergothioneine, 0.01-8% hydroxyethylpiperazine ethanesulfonic acid, and 0.01-1.5% creatine; preferably 0.2-2% ergothioneine, 0.2-8% hydroxyethylpiperazine ethanesulfonic acid, and 0.2-1.5% creatine.

[0041] For example, the ergothioneine content could be 0.01%, 0.05%, 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5%, 0.55%, 0.6%, 0.65%, 0.7%, 0.75%, 0.8%, 0.85%, 0.9%, 0.95%, 1%, 1.05%, 1.1%, 1.15%, 1.2%, 1.25%, 1.3%, 1.35%, 1.4%, 1.45%, 1.5%, 1.55%, 1.6%, 1.65%, 1.7%, 1.75%, 1.8%, 1.85%, 1.9%, 1.95%, or 2%.

[0042] For example, the hydroxyethylpiperazine ethanesulfonic acid content could be 0.01%, 0.25%, 0.5%, 0.75%, 1%, 1.25%, 1.5%, 1.75%, 2%, 2.25%, 2.5%, 2.75%, 3%, 3.25%, 3.5%, 3.75%, 4%, 4.25%, 4.5%, 4.75%, 5%, 5.25%, 5.5%, 5.75%, 6%, 6.25%, 6.5%, 6.75%, 7%, 7.25%, 7.5%, 7.75%, or 8%.

[0043] For example, the creatine content could be 0.01%, 0.05%, 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5%, 0.55%, 0.6%, 0.65%, 0.7%, 0.75%, 0.8%, 0.85%, 0.9%, 0.95%, 1%, 1.05%, 1.1%, 1.15%, 1.2%, 1.25%, 1.3%, 1.35%, 1.4%, 1.45%, or 1.5%.

[0044] In one specific embodiment of this application, when used to improve the stability of N-acetylneuraminic acid, the ratio of N-acetylneuraminic acid to ergothioneine is 0.2:(0.01-2); preferably 0.2:(0.2-2).

[0045] For example, the ratio of N-acetylneuraminic acid to ergothioneine could be 0.2:0.01, 0.2:0.05, 0.2:0.1, 0.2:0.15, 0.2:0.2, 0.2:0.25, 0.2:0.3, 0.2:0.35, 0.2:0.4, 0.2:0.45, 0.2:0.5, 0.2:0.55, 0.2:0.6, 0.2:0.65, 0.2:0.7, 0.2:0.75, 0.2:0.8, 0.2:0.85, or 0.2:0. 9, 0.2:0.95, 0.2:1, 0.2:1.05, 0.2:1.1, 0.2:1.15, 0.2:1.2, 0.2:1.25, 0.2:1.3, 0.2:1.35, 0.2:1.4, 0.2:1.45, 0.2:1.5, 0.2:1.55, 0.2:1.6, 0.2:1.65, 0.2:1.7, 0.2:1.75, 0.2:1.8, 0.2:1.85, 0.2:1.9, 0.2:1.95, 0.2:2;

[0046] The preferred ratio of N-acetylneuraminic acid to ergothioneine is 0.2:0.2, 0.2:0.25, 0.2:0.3, 0.2:0.35, 0.2:0.4, 0.2:0.45, 0.2:0.5, 0.2:0.55, 0.2:0.6, 0.2:0.65, 0.2:0.7, 0.2:0.75, 0.2:0.8, 0.2:0.85, 0.2:0.9, 0.2:0.95, 0.2:1, or 0. 2:1.05, 0.2:1.1, 0.2:1.15, 0.2:1.2, 0.2:1.25, 0.2:1.3, 0.2:1.35, 0.2:1.4, 0.2:1.45, 0.2:1.5, 0.2:1.55, 0.2:1.6, 0.2:1.65, 0.2:1.7, 0.2:1.75, 0.2:1.8, 0.2:1.85, 0.2:1.9, 0.2:1.95, 0.2:2.

[0047] In one specific embodiment of this application, when used to improve the stability of N-acetylneuraminic acid, the ratio of N-acetylneuraminic acid to hydroxyethylpiperazine ethanesulfonic acid is 0.2:(0.01-8); preferably 0.2:(0.2-8).

[0048] For example, the ratio of N-acetylneuraminic acid to hydroxyethylpiperazine ethanesulfonic acid could be 0.2:0.01, 0.2:0.2, 0.2:0.25, 0.2:0.5, 0.2:0.75, 0.2:1, 0.2:1.25, 0.2:1.5, 0.2:1.75, 0.2:2, 0.2:2.25, 0.2:2.5, 0.2:2.75, 0.2:3, or 0.2:3.25. 0.2:3.5, 0.2:3.75, 0.2:4, 0.2:4.25, 0.2:4.5, 0.2:4.75, 0.2:5, 0.2:5.25, 0.2:5.5, 0.2:5.75, 0.2:6, 0.2:6.25, 0.2:6.5, 0.2:6.75, 0.2:7, 0.2:7.25, 0.2:7.5, 0.2:7.75, 0.2:8;

[0049] The preferred ratio of N-acetylneuraminic acid to hydroxyethylpiperazine ethanesulfonic acid is 0.2:0.2, 0.2:0.25, 0.2:0.5, 0.2:0.75, 0.2:1, 0.2:1.25, 0.2:1.5, 0.2:1.75, 0.2:2, 0.2:2.25, 0.2:2.5, 0.2:2.75, 0.2:3, 0.2:3.25, or 0.2:3. 5, 0.2:3.75, 0.2:4, 0.2:4.25, 0.2:4.5, 0.2:4.75, 0.2:5, 0.2:5.25, 0.2:5.5, 0.2:5.75, 0.2:6, 0.2:6.25, 0.2:6.5, 0.2:6.75, 0.2:7, 0.2:7.25, 0.2:7.5, 0.2:7.75, 0.2:8.

[0050] In one specific embodiment of this application, when used to improve the stability of N-acetylneuraminic acid, the ratio of N-acetylneuraminic acid to creatine is 0.2:(0.01 to 1.5); preferably 0.2:(0.2 to 1.5).

[0051] For example, the ratio of N-acetylneuraminic acid to creatine could be 0.2:0.01, 0.2:0.05, 0.2:0.1, 0.2:0.15, 0.2:0.2, 0.2:0.25, 0.2:0.3, 0.2:0.35, 0.2:0.4, 0.2:0.45, 0.2:0.5, 0.2:0.55, 0.2:0.6, or 0.2:0.65. 0.2:0.7, 0.2:0.75, 0.2:0.8, 0.2:0.85, 0.2:0.9, 0.2:0.95, 0.2:1, 0.2:1.05, 0.2:1.1, 0.2:1.15, 0.2:1.2, 0.2:1.25, 0.2:1.3, 0.2:1.35, 0.2:1.4, 0.2:1.45, 0.2:1.5;

[0052] The preferred ratio of N-acetylneuraminic acid to creatine is 0.2:0.2, 0.2:0.25, 0.2:0.3, 0.2:0.35, 0.2:0.4, 0.2:0.45, 0.2:0.5, 0.2:0.55, 0.2:0.6, 0.2:0.65, 0.2:0.7, 0.2:0.75, 0.2:0.8, 0.2:0.85, 0.2:0.9, 0.2:0.95, 0.2:1, 0.2:1.05, 0.2:1.1, 0.2:1.15, 0.2:1.2, 0.2:1.25, 0.2:1.3, 0.2:1.35, 0.2:1.4, 0.2:1.45, or 0.2:1.5.

[0053] This application also provides a composition comprising N-acetylneuraminic acid and the aforementioned composition for improving the stability of N-acetylneuraminic acid.

[0054] This application provides a composition comprising N-acetylneuraminic acid and creatine; further, in the composition, only creatine is used as a stabilizer for N-acetylneuraminic acid.

[0055] In one specific embodiment of this application, the percentage content of each component in the composition satisfies the following ratio: N-acetylneuraminic acid to ergothioneine, hydroxyethylpiperazine ethanesulfonic acid and creatine is 0.2:(0.01-2):(0.01-8):(0.01-1.5);

[0056] For example, the ratio of N-acetylneuraminic acid to ergothioneine could be 0.2:0.01, 0.2:0.05, 0.2:0.1, 0.2:0.15, 0.2:0.2, 0.2:0.25, 0.2:0.3, 0.2:0.35, 0.2:0.4, 0.2:0.45, 0.2:0.5, 0.2:0.55, 0.2:0.6, 0.2:0.65, 0.2:0.7, 0.2:0.75, 0.2:0.8, 0.2:0.85, or 0.2:0. 9, 0.2:0.95, 0.2:1, 0.2:1.05, 0.2:1.1, 0.2:1.15, 0.2:1.2, 0.2:1.25, 0.2:1.3, 0.2:1.35, 0.2:1.4, 0.2:1.45, 0.2:1.5, 0.2:1.55, 0.2:1.6, 0.2:1.65, 0.2:1.7, 0.2:1.75, 0.2:1.8, 0.2:1.85, 0.2:1.9, 0.2:1.95, 0.2:2;

[0057] For example, the ratio of N-acetylneuraminic acid to hydroxyethylpiperazine ethanesulfonic acid could be 0.2:0.01, 0.2:0.25, 0.2:0.5, 0.2:0.75, 0.2:1, 0.2:1.25, 0.2:1.5, 0.2:1.75, 0.2:2, 0.2:2.25, 0.2:2.5, 0.2:2.75, 0.2:3, 0.2:3.25, 0.2 3.5, 0.2:3.75, 0.2:4, 0.2:4.25, 0.2:4.5, 0.2:4.75, 0.2:5, 0.2:5.25, 0.2:5.5, 0.2:5.75, 0.2:6, 0.2:6.25, 0.2:6.5, 0.2:6.75, 0.2:7, 0.2:7.25, 0.2:7.5, 0.2:7.75, 0.2:8;

[0058] For example, the ratio of N-acetylneuraminic acid to creatine could be 0.2:0.01, 0.2:0.05, 0.2:0.1, 0.2:0.15, 0.2:0.2, 0.2:0.25, 0.2:0.3, 0.2:0.35, 0.2:0.4, 0.2:0.45, 0.2:0.5, 0.2:0.55, 0.2:0.6, or 0.2:0.65. 0.2:0.7, 0.2:0.75, 0.2:0.8, 0.2:0.85, 0.2:0.9, 0.2:0.95, 0.2:1, 0.2:1.05, 0.2:1.1, 0.2:1.15, 0.2:1.2, 0.2:1.25, 0.2:1.3, 0.2:1.35, 0.2:1.4, 0.2:1.45, 0.2:1.5.

[0059] This application provides a composition comprising N-acetylneuraminic acid, hydroxyethylpiperazine ethanesulfonic acid, and creatine, wherein the weight percentage of N-acetylneuraminic acid is 0.2 parts, the weight percentage of ergothioneine is 0.01 to 2 parts, the weight percentage of hydroxyethylpiperazine ethanesulfonic acid is 0.01 to 8 parts, the weight percentage of creatine is 0.01 to 1.5 parts, and the balance is water.

[0060] This application discloses a composition containing N-acetylneuraminic acid. When ergothioneine and hydroxyethylpiperazine ethanesulfonic acid are combined as a stabilizer to improve the stability of N-acetylneuraminic acid, the ergothioneine and hydroxyethylpiperazine ethanesulfonic acid have an antagonistic effect, thereby reducing the effect of the stabilizer to a certain extent.

[0061] In this application, when ergothioneine and hydroxyethylpiperazine ethanesulfonic acid are combined as a stabilizer to improve the stability of N-acetylneuraminic acid based on a composition containing N-acetylneuraminic acid, the antagonistic effect is significantly reduced when the content ratio of ergothioneine to hydroxyethylpiperazine ethanesulfonic acid is (0.001-0.1):(10-200).

[0062] In this application, when discussing the degree of discoloration, the color of the solution observed by the composition on day 40 is used as the standard.

[0063] Example

[0064] Unless otherwise specified, the experimental methods used in the following examples are conventional methods. The color change time was observed and recorded visually at the same time every day.

[0065] Unless otherwise specified, all materials and reagents used in the following examples are commercially available.

[0066] Example 1

[0067] The composition in this embodiment consists of 0.2g N-acetylneuraminic acid (sialic acid) and 0.2g hydroxyethylpiperazine ethanesulfonic acid, with water added to bring the total composition to 100g. The composition was placed in a 50°C oven, and the results were checked periodically and recorded in Table 1.

[0068] Example 2

[0069] The composition in this embodiment consists of 0.2g N-acetylneuraminic acid (sialic acid) and 0.2g ergothioneine, with water added to bring the total composition to 100g. The composition was placed in a 50°C oven, and the results were checked periodically and recorded in Table 1.

[0070] Example 3

[0071] The composition in this embodiment consists of 0.2g N-acetylneuraminic acid (sialic acid) and 0.2g creatine, with water added to bring the total composition to 100g. The composition was placed in a 50°C oven, and the results were checked periodically and recorded in Table 1.

[0072] Example 4

[0073] The difference between this embodiment and Example 1 is that 0.2g of hydroxyethylpiperazine ethanesulfonic acid is replaced with 2g.

[0074] Example 5

[0075] The difference between this embodiment and Embodiment 1 is that 0.2g of hydroxyethylpiperazine ethanesulfonic acid is replaced with 8g.

[0076] Example 6

[0077] The difference between this embodiment and Embodiment 2 is that 0.2g of ergothioneine is replaced with 2g of ergothioneine.

[0078] Example 7

[0079] The composition in this embodiment consists of 0.2g N-acetylneuraminic acid (sialic acid) and 1.5g creatine, with water added to bring the total composition to 100g. The composition was placed in a 50°C oven, and the results were checked periodically and recorded in Table 1.

[0080] Example 8

[0081] The composition in this embodiment consists of 0.2g N-acetylneuraminic acid (sialic acid), 1g creatine, and water is added to bring the composition to 100g. The composition is placed in a 50°C oven, and the results are checked periodically and recorded in Table 1.

[0082] Example 9

[0083] The composition in this embodiment consists of 0.2g N-acetylneuraminic acid (sialic acid) and 0.5g creatine, with water added to bring the total composition to 100g. The composition was placed in a 50°C oven, and the results were checked periodically and recorded in Table 1.

[0084] Example 10

[0085] The composition in this embodiment consists of 0.2g N-acetylneuraminic acid (sialic acid), 0.2g hydroxyethylpiperazine ethanesulfonic acid, 0.2g ergothioneine, and 0.2g creatine, with water added to bring the composition to 100g. The composition was placed in a 50°C oven, and the results were checked periodically and recorded in Table 1.

[0086] Example 11

[0087] The difference between this embodiment and Example 10 is that the content of hydroxyethylpiperazine ethanesulfonic acid is 0.01g.

[0088] Example 12

[0089] The difference between this embodiment and Embodiment 10 is that the content of ergothioneine is 0.01g.

[0090] Example 13

[0091] The difference between this embodiment and Embodiment 10 is that the creatine content is 0.01g.

[0092] Example 14

[0093] The composition in this embodiment consists of 0.2g N-acetylneuraminic acid (sialic acid), 8g hydroxyethylpiperazine ethanesulfonic acid, 0.01g ergothioneine, and 0.01g creatine, with water added to bring the composition to 100g. The composition was placed in a 50°C oven, and the results were checked periodically and recorded in Table 1.

[0094] Example 15

[0095] The composition in this embodiment consists of 0.2g N-acetylneuraminic acid (sialic acid), 0.01g hydroxyethylpiperazine ethanesulfonic acid, 0.01g ergothioneine, and 1.5g creatine, with water added to bring the composition to 100g. The composition was placed in a 50°C oven, and the results were checked periodically and recorded in Table 1.

[0096] Example 16

[0097] The composition in this embodiment consists of 0.2g N-acetylneuraminic acid (sialic acid), 0.01g hydroxyethylpiperazine ethanesulfonic acid, 2g ergothioneine, and 0.01g creatine, with water added to bring the composition to 100g. The composition was placed in a 50°C oven, and the results were checked periodically and recorded in Table 1.

[0098] Example 17

[0099] The composition in this embodiment consists of 0.2g N-acetylneuraminic acid (sialic acid), 0.01g hydroxyethylpiperazine ethanesulfonic acid, 0.01g ergothioneine, and 0.01g creatine, with water added to bring the composition to 100g. The composition was placed in a 50°C oven, and the results were checked periodically and recorded in Table 1.

[0100] Comparative Example 1

[0101] The composition in this comparative example contained only 0.2 g of N-acetylneuraminic acid (sialic acid), and water was added to bring the composition to 100 g. The composition was placed in a 50°C oven, and the results were checked periodically and recorded in Table 1.

[0102] Comparative Example 2

[0103] The composition in this comparative example consisted of 0.2 g N-acetylneuraminic acid (sialic acid) and 0.01 g hydroxyethylpiperazine ethanesulfonic acid, with water added to bring the total composition to 100 g. The composition was placed in a 50°C oven, and the results were checked periodically and recorded in Table 1.

[0104] Comparative Example 3

[0105] The composition in this comparative example consisted of 0.2 g N-acetylneuraminic acid (sialic acid) and 1.5 g hydroxyethylpiperazine ethanesulfonic acid, with water added to bring the total composition to 100 g. The composition was placed in a 50°C oven, and the results were checked periodically and recorded in Table 1.

[0106] Comparative Example 4

[0107] The composition in this comparative example consisted of 0.2 g N-acetylneuraminic acid (sialic acid) and 0.01 g ergothioneine, with water added to bring the total composition to 100 g. The composition was placed in a 50°C oven, and the results were checked periodically and recorded in Table 1.

[0108] Comparative Example 5

[0109] The composition in this comparative example consisted of 0.2 g N-acetylneuraminic acid (sialic acid) and 8 g ergothioneine, with water added to bring the total composition to 100 g. The composition was placed in a 50°C oven, and the results were checked periodically and recorded in Table 1.

[0110] Comparative Example 6

[0111] The composition in this comparative example consisted of 0.2 g N-acetylneuraminic acid (sialic acid) and 0.01 g creatine, with water added to bring the total composition to 100 g. The composition was placed in a 50°C oven, and the results were checked periodically and recorded in Table 1.

[0112] Comparative Example 7

[0113] The composition in this comparative example consisted of 0.2 g of N-acetylneuraminic acid (sialic acid) and 2.5 g of creatine, with water added to bring the total composition to 100 g. After preparation, the composition showed obvious precipitation, and the results are recorded in Table 1.

[0114] Table 1. Color change time and degree of color change for each embodiment and comparative example.

[0115]

[0116]

[0117] Note: The more asterisks (*), the darker the color of the composition at 40 days; "—" indicates that the composition will not change color; " / " indicates that the composition does not contain this component.

[0118] Based on Examples 1-3 and Comparative Example 1, it can be seen that using an appropriate amount (0.2g) of hydroxyethylpiperazine ethanesulfonic acid, ergothioneine, or creatine alone can delay the discoloration time of the composition to a certain extent. Hydroxyethylpiperazine ethanesulfonic acid and creatine can reduce the degree of discoloration of the composition to varying degrees, with creatine being particularly effective; ergothioneine aggravates the degree of discoloration of the composition, even more so than the discoloration in Comparative Example 1 without stabilizers.

[0119] As shown in Examples 1, 4, and 5, the color change time gradually slows down with increasing dosage of hydroxyethylpiperazine ethanesulfonic acid until the hydroxyethylpiperazine ethanesulfonic acid reaches a certain mass, at which point the color change time of the composition no longer changes; however, the degree of color change of the composition continuously decreases with increasing dosage of hydroxyethylpiperazine ethanesulfonic acid. Referring to Comparative Example 3, when an excessive amount (15g) of hydroxyethylpiperazine ethanesulfonic acid is added, the color change time of the composition is slightly delayed compared to Example 5, but the degree of color change is deeper than that of Example 5. Referring to Comparative Example 2, when a trace amount (0.01g) of hydroxyethylpiperazine ethanesulfonic acid is added, it has no significant effect on the color change time of the composition; on the contrary, it intensifies the degree of color change.

[0120] Based on Examples 2 and 6 and Comparative Examples 4 and 5, it can be seen that when ergothioneine is used at 0.2g and 2g, it can delay the color change time of the composition to a certain extent. When ergothioneine is used at 2g, the color change of the composition is lighter than that of Comparative Example 1. In other cases, the color change of the composition is darker. When the amount of ergothioneine used is 0.2g and 2g, the color change time can be delayed by 7 days and 2 days, respectively. In other cases, no significant delay in color change time was observed.

[0121] Based on Examples 7-9 and Comparative Examples 1, 6, and 7, it can be seen that when the amount of creatine added is between 0.5 and 1.5 g, the composition never changed color until 40 days later. When the amount of creatine added is too low (0.01 g), creatine does not significantly delay the color change time of the composition; on the contrary, it makes the degree of color change more severe compared to Comparative Example 1. When the amount of creatine added is too high (2.5 g), obvious precipitation can be observed in the composition at room temperature (20°C) after preparation, indicating that creatine is insoluble in the composition.

[0122] As can be seen from Examples 10-13, when the contents of ergothioneine and hydroxyethylpiperazine ethanesulfonic acid are similar in Examples 10 and 13, there is an antagonistic effect, which reduces the effect of the stabilizer to a certain extent. Since there is an appropriate amount (0.2g) of creatine in Example 10, the composition can still maintain its color change for 25 days. However, in Example 13, due to the low content of creatine (0.01g), the stability of N-acetylneuraminic acid in the composition cannot be significantly improved, and the color change time can only be delayed by 3 days, but the color change is deeper than that of Comparative Example 1.

[0123] In summary, when the contents of ergothioneine and hydroxyethylpiperazine ethanesulfonic acid are similar, there will be an antagonistic effect, which will reduce the effect of the stabilizer to a certain extent. When the creatine content is high, the effect of the antagonistic effect can be compensated, and the discoloration time can still be significantly delayed and the degree of discoloration can be reduced.

[0124] Based on Examples 14 and 5, it can be seen that adding trace amounts of ergothioneine and creatine (Example 14) to the addition of 8g of hydroxyethylpiperazine ethanesulfonic acid can significantly delay the discoloration time compared to Example 5, but will slightly aggravate the degree of discoloration.

[0125] Based on Examples 15 and 16 and Examples 6 and 7, it can be seen that when sufficient creatine or ergothioneine is added, the addition of a trace amount of another stabilizer (0.01g) has no significant effect on delaying the color change time. The color change shown in Example 16 is lighter than that in Example 6.

[0126] As can be seen from Example 17, when the three stabilizers are added in trace amounts (0.01g) in combination, the color change time is delayed by only 2 days compared to Comparative Example 1, and is accompanied by the negative effect of increased color change of the composition.

[0127] Based on Comparative Examples 1-3 and Example 5, it can be seen that both excessively high (15g) and excessively low (0.01g) amounts of hydroxyethylpiperazine ethane sulfonic acid will aggravate the discoloration of the composition compared to Example 5. At the same time, when the amount added is too high (15g), the discoloration time can be delayed by 3 days compared to Example 5.

[0128] Based on Comparative Examples 1, 4, and 5, it can be seen that when the amount of ergothionein added is too high (8g) or too low (0.01g), it does not delay the color change time and will aggravate the color change compared to Comparative Example 1.

[0129] Based on Comparative Examples 1, 6, and 7, it can be seen that when the amount of creatine added is too low (0.01g), it will not delay the color change time of the composition, but will aggravate the color change degree of the composition to a certain extent; when the amount of creatine added is too high (2.5g), the composition will show obvious precipitation.

[0130] Although the embodiments of this application have been described above, this application is not limited to the specific embodiments and application fields described above. The specific embodiments described above are merely illustrative and instructive, and not restrictive. Those skilled in the art can make many other forms based on the guidance of this specification and without departing from the scope of protection of the claims of this application, and these are all within the scope of protection of this application.

Claims

1. A composition for improving the stability of N-acetylneuraminic acid, comprising: Creatine.

2. The composition according to claim 1, wherein, Also includes: Ergothioneine, and / or Hydroxyethylpiperazine ethane sulfonic acid.

3. The composition according to claim 1, wherein, When used to improve the stability of N-acetylneuraminic acid, the ratio of creatine to N-acetylneuraminic acid is (0.01–1.5):0.2; preferably, the ratio of creatine to N-acetylneuraminic acid is (0.2–1.5):0.

2.

4. The composition according to claim 2, wherein, When used to improve the stability of N-acetylneuraminic acid, the ratio of N-acetylneuraminic acid to ergothioneine and / or hydroxyethylpiperazine ethanesulfonic acid is 0.2:(0.01-2) and / or 0.2:(0.01-8); preferably, the ratio of N-acetylneuraminic acid to ergothioneine and / or hydroxyethylpiperazine ethanesulfonic acid is 0.2:(0.2-2) and / or 0.2:(0.2-8).

5. Use of creatine to improve the stability of N-acetylneuraminic acid.

6. Use of creatine, ergothioneine, and / or hydroxyethylpiperazine ethanesulfonic acid in combination to improve the stability of N-acetylneuraminic acid.

7. A composition wherein, include: N-acetylneuraminic acid, and Creatine.

8. A composition comprising: N-acetylneuraminic acid, and creatine; wherein the composition comprises only creatine as a stabilizer for N-acetylneuraminic acid.

9. The composition according to claim 7, wherein, Also includes: Ergothioneine, and / or Hydroxyethylpiperazine ethane sulfonic acid.

10. The composition according to claim 7 or 8, wherein, The percentage content of each component in the composition satisfies the requirement that the ratio of N-acetylneuraminic acid to creatine is 0.2:(0.01-1.5); preferably, the ratio of N-acetylneuraminic acid to creatine is 0.2:(0.2-1.5).

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