Sample tank of multifunctional PCR (Polymerase Chain Reaction) gene amplification instrument

Abstract

The invention discloses a sample groove of a multifunctional PCR gene amplification instrument, and relates to the technical field of amplification instrument parts, the sample groove comprises a placing assembly, a sample seat, a first spring arranged in the sample seat, a sample test tube arranged at the top of the sample seat, a stabilizing piece arranged at the top of the sample seat, and a bearing plate located at the top of the sample seat, and a bearing groove is formed in the top of the bearing plate. The device has the beneficial effects that through the arrangement of the placing assembly and the groove cover assembly, after the sample test tube is placed, the sample test tube sinking into the sample seat can be positioned and prevented from falling off in cooperation with the closing action of the cover plate, so that the placing stability of the sample test tube is effectively improved, and stable heat exchange between the sample seat and the sample test tube is facilitated; and when the cover plate is opened, the protection piece automatically completes stretching and blocking actions, so that an operator can be prevented from being scalded by touching the auxiliary heating plate by mistake, and the operation safety is greatly improved.

Description

Technical Field

[0001] This invention relates to the field of amplification instrument components, and in particular to a sample holder for a multifunctional PCR gene amplification instrument. Background Technology

[0002] A PCR gene amplification instrument, also known as a PCR amplification instrument, PCR nucleic acid amplification instrument, or polymerase chain reaction nucleic acid amplification instrument, is an instrument that uses PCR technology to amplify specific DNA. It is widely used in medical and biological laboratories and usually consists of a heated lid, a heated circulation system, a transmission system, a control system, and a power supply system. The sample holder is an essential component, used to be installed inside the device to hold multiple test tubes and, together with the heated lid, to place and install DNA samples.

[0003] In existing technologies, sample slots typically have multiple holes for placing test tubes. These, along with the thermal circulation components in the amplification instrument, ensure that the sample slots uniformly conduct heat to the test tubes. However, as the temperature changes continuously during the denaturation, annealing, and extension steps, the test tubes tend to adhere tightly to the sample slot holes due to thermal expansion, increasing contact friction. While this helps improve the uniformity of the amplification process, it also makes it difficult to remove the test tubes after processing. They must be allowed to cool naturally before being removed with the aid of special clamps. Overall, the processing time is long, and the processing efficiency is generally low. Summary of the Invention

[0004] In view of the problems existing in the sample chambers of the current multifunctional PCR gene amplification instrument, the present invention is proposed.

[0005] Therefore, the problem that this invention aims to solve is that in the prior art, as the temperature changes continuously during the three steps of denaturation, annealing and stretching, the test tube itself is prone to sticking tightly to the sample slot hole due to thermal expansion, which increases the contact friction and makes it difficult to remove the test tube after processing, resulting in generally low overall processing efficiency.

[0006] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a sample holder for a multifunctional PCR gene amplification instrument, comprising, A placement assembly includes a sample holder, a first spring disposed within the sample holder, a sample tube disposed on the top of the sample holder, a stabilizing component disposed on the top of the sample holder, a support plate located on the top of the sample holder, a support groove formed on the top of the support plate, a clamping component disposed on the top of the support plate, a pressure frame disposed on the top of the support plate, and sliding rods slidably connected to the four corners of the sample holder; and... A heating plate is located at the bottom of the sample holder; and, The sample holder assembly, located at the top of the sample holder, includes a cover plate disposed outside the sample holder. An auxiliary heating plate is fixed inside the cover plate. A protective component is provided on the side of the auxiliary heating plate away from the cover plate, including a fixing plate fixed to the cover plate. A sliding plate is slidably connected to the fixing plate. A pressure rod is fixed to the fixing plate. A movable strip is rotatably connected to the sliding plate. A displacement frame is provided on the outside of the fixing plate. A displacement block is fixed on the displacement frame. Auxiliary blocks are provided on both sides of the fixing plate.

[0007] As a preferred embodiment of the sample slot of the multifunctional PCR gene amplification instrument of the present invention, the sample holder has a test tube slot that cooperates with the sample test tube. The bottom end of the first spring is fixed in the test tube slot, and a heat-conducting ring is fixed at the top end of the first spring. A fifth spring is sleeved on the slide rod, and the top end of the fifth spring is fixed on the sample holder, while the bottom end of the fifth spring is fixed on the slide rod.

[0008] As a preferred embodiment of the sample slot of the multifunctional PCR gene amplification instrument of the present invention, the support plate is slidably connected to the slide rod, a second spring is fixed at the bottom of the support plate, the other end of the second spring is fixed to the slide rod, and the pressure frame is fixed to the slide rod.

[0009] As a preferred embodiment of the sample slot of the multifunctional PCR gene amplification instrument of the present invention, the clamping component includes a protrusion fixed to the top of the support plate, a pressing block is provided on the top of the protrusion, and a third spring is fixed on the pressing block.

[0010] As a preferred embodiment of the sample slot of the multifunctional PCR gene amplification instrument of the present invention, wherein: the other end of the third spring is fixed to the top of the support plate, a guide block is fixed to the top of the protrusion, and a protrusion is provided on the clamping member and cooperates with the guide block.

[0011] As a preferred embodiment of the sample slot of the multifunctional PCR gene amplification instrument of the present invention, the cover plate is provided with an installation slot, the auxiliary heating plate is fixed in the center of the installation slot, a first storage slot is provided on both sides of the installation slot and cooperates with the auxiliary block, and a second storage slot is provided in the first storage slot and cooperates with the first storage slot.

[0012] As a preferred embodiment of the sample slot of the multifunctional PCR gene amplification instrument of the present invention, the fixing plate is located outside the auxiliary heating plate, the pressure rod corresponds to the sample tube, and both the fixing plate and the sliding plate are provided with through slots.

[0013] As a preferred embodiment of the sample slot of the multifunctional PCR gene amplification instrument of the present invention, wherein: a fourth spring is fixed on one side of the auxiliary block, the other end of the fourth spring is fixed in the first receiving slot, and the end of the movable strip away from the slide plate is rotatably connected to the auxiliary block.

[0014] As a preferred embodiment of the sample slot of the multifunctional PCR gene amplification instrument of the present invention, the top and bottom of the fixing plate are provided with sliding grooves, which cooperate with the sliding plate.

[0015] As a preferred embodiment of the sample holder of the multifunctional PCR gene amplification instrument of the present invention, the bottom of the sample holder is in contact with the top of the heating plate, and the bottom of the sample holder is provided with a heat equalization hole and is offset from the test tube slot.

[0016] The beneficial effects of this invention are as follows: By setting up the placement component and the tank cover component, the sample tubes can be pre-compressed after placement, and the closing action of the cover plate can be used to position and prevent the sample tubes sunk into the sample holder from falling out. This effectively improves the stability of sample tube placement, facilitates stable heat exchange between the sample holder and the sample tubes, ensures the amplification effect, and when the cover plate is opened, the protective component automatically completes the extension and blocking action, which can prevent operators from accidentally touching the auxiliary heating plate and getting burned, greatly improving operational safety. At the same time, no additional electrical equipment is required, which is more in line with actual processing needs. Attached Figure Description

[0017] To more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the following description of the embodiments will be briefly introduced. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This is a structural diagram of the sample well of a multifunctional PCR gene amplification instrument.

[0019] Figure 2 This is a structural diagram of the sample holder and heating plate of a multifunctional PCR gene amplification instrument.

[0020] Figure 3 This is a cross-sectional view of the sample holder in the sample compartment of a multifunctional PCR gene amplification instrument.

[0021] Figure 4 This is a top view of the sample holder in the sample compartment of a multifunctional PCR gene amplification instrument.

[0022] Figure 5 This is a diagram showing the separation of the sample holder, stabilizer, and heating plate in the sample compartment of a multifunctional PCR gene amplification instrument.

[0023] Figure 6 This is a partial structural separation diagram of the stabilizer component of the sample chamber of a multifunctional PCR gene amplification instrument.

[0024] Figure 7This is another perspective view of the sample holder plate and pressure frame of a multifunctional PCR gene amplification instrument when they separate.

[0025] Figure 8 For the sample holder of a multifunctional PCR gene amplification instrument Figure 7 Enlarged view of point A in the middle.

[0026] Figure 9 This is a diagram showing the separation of the sample compartment cover assembly of a multifunctional PCR gene amplification instrument.

[0027] Figure 10 This is a top sectional view of the sample well cover assembly of a multifunctional PCR gene amplification instrument.

[0028] Figure 11 This is a diagram showing the installation of the sample holder fixing plate and displacement frame for a multifunctional PCR gene amplification instrument.

[0029] Figure 12 This is a structural diagram of the fixing plate of the sample slot in a multifunctional PCR gene amplification instrument.

[0030] Figure 13 For the sample holder of a multifunctional PCR gene amplification instrument Figure 12 Enlarged view of section B in the middle.

[0031] In the diagram: 100, Placement component; 101, Sample holder; 101a, Test tube slot; 101b, First spring; 101b-1, Heat-conducting ring; 101c, Heat-spreading hole; 102, Sample test tube; 103, Stabilizer; 103a, Support plate; 103a-1, Support groove; 103a-2, Second spring; 103a-3, Clamping component; 103a-3a, Thrust; 103a-3b, Pressing block; 103a-3c, Third spring; 103a-3d, Guide block; 103a-3e, Guide groove; 103b, Pressure frame; 10 3c, sliding rod; 103c-1, fifth spring; 200, heating plate; 300, slot cover assembly; 301, cover plate; 301a, mounting slot; 301b, first storage slot; 301c, second storage slot; 302, auxiliary heating plate; 303, protective component; 303a, fixing plate; 303a-1, sliding plate; 303a-2, pressure rod; 303a-3, movable strip; 303a-4, through slot; 303a-5, sliding groove; 303b, displacement frame; 303b-1, displacement block; 303c, auxiliary block; 303c-1, fourth spring. Detailed Implementation

[0032] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0033] Many specific details are set forth in the following description in order to provide a full understanding of the invention. However, the invention may also be practiced in other ways different from those described herein, and those skilled in the art can make similar extensions without departing from the spirit of the invention. Therefore, the invention is not limited to the specific embodiments disclosed below.

[0034] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it a single or selective embodiment that is mutually exclusive with other embodiments. Example 1

[0035] Reference Figure 1 and Figure 2 This is the first embodiment of the present invention. This embodiment provides a sample holder for a multifunctional PCR gene amplification instrument. The sample holder of the multifunctional PCR gene amplification instrument includes a placement component 100, a heating plate 200, and a cover component 300. By setting the placement component 100 and the cover component 300, the sample can be pre-compressed after placement, in conjunction with the closing action of the PCR amplification instrument, to achieve the purpose of positioning and preventing detachment, effectively improving the stability of sample placement, facilitating stable heat conduction, ensuring the amplification effect, and automatically completing the extension and blocking action when the PCR amplification instrument is turned on, which can avoid accidental burns to the operator and greatly improve operational safety. The working principle of the opening and closing action of the PCR amplification instrument is the prior art and will not be described in detail here, but will be clearly understood by those skilled in the art.

[0036] Specifically, the placement component 100 includes a sample holder 101, a first spring 101b inside the sample holder 101, a sample tube 102 on the top of the sample holder 101, a stabilizing component 103 on the top of the sample holder 101, a support plate 103a on the top of the sample holder 101, a support groove 103a-1 on the top of the support plate 103a, a clamping component 103a-3 on the top of the support plate 103a, a pressure frame 103b on the top of the support plate 103a, and sliding rods 103c slidably connected to the four corners of the sample holder 101.

[0037] The pressure frame 103b is located on top of the support plate 103a. Through the setting of the stabilizer 103, it can initially support the sample tube 102 and complete the anti-detachment protection as the equipment is closed, effectively improving the placement stability of the sample tube 102.

[0038] Specifically, the heating plate 200 is located at the bottom of the sample holder 101.

[0039] The top of the heating plate 200 is in direct contact with the bottom of the sample holder 101, so that heat can be evenly transferred to the sample holder 101 when the heating plate 200 is in operation.

[0040] Specifically, the slot cover assembly 300 is located on top of the sample holder 101 and includes a cover plate 301 located outside the sample holder 101. An auxiliary heating plate 302 is fixed inside the cover plate 301. A protective component 303 is provided on the side of the auxiliary heating plate 302 away from the cover plate 301, including a fixing plate 303a fixed to the cover plate 301. A sliding plate 303a-1 is slidably connected to the fixing plate 303a. A pressure rod 303a-2 is fixed to the fixing plate 303a. A movable strip 303a-3 is rotatably connected to the sliding plate 303a-1. A displacement frame 303b is provided on the outside of the fixing plate 303a. A displacement block 303b-1 is fixed on the displacement frame 303b. Auxiliary blocks 303c are provided on both sides of the fixing plate 303a.

[0041] The displacement block 303b-1 is tilted on the side near the auxiliary block 303c, as shown in the attached diagram of the instruction manual. Figure 13 As shown, with this design, when the displacement block 303b-1 comes into contact with the auxiliary block 303c, it will squeeze the auxiliary block 303c to move outward.

[0042] The displacement block 303b-1 is located between the upper and lower movable bars 303a-3. This design can prevent the displacement block 303b-1 from contacting and obstructing the movable bar 303a-3 when it is displaced.

[0043] By setting the protective component 303, when the cover plate 301 is rotated to close, the two sliding plates 303a-1 retract into the fixed plate 303a, so that the auxiliary heating plate 302 is exposed over a large area. Thus, when the auxiliary heating plate 302 is working, it can achieve the purpose of auxiliary heating of the top of the sample tube 102. When the cover plate 301 is rotated to open, the two sliding plates 303a-1 gradually unfold, which can cover the auxiliary heating plate 302 over a large area, so as to avoid burns to the hands of personnel when they touch the auxiliary heating plate 302. Example 2

[0044] Reference Figures 2 to 13 This is the second embodiment of the present invention, which is based on the previous embodiment.

[0045] Specifically, a test tube slot 101a is provided in the sample holder 101 and cooperates with the sample test tube 102. The bottom end of the first spring 101b is fixed in the test tube slot 101a, and a heat-conducting ring 101b-1 is fixed at the top end of the first spring 101b. A fifth spring 103c-1 is sleeved on the slide rod 103c. The top end of the fifth spring 103c-1 is fixed on the sample holder 101, and the bottom end of the fifth spring 103c-1 is fixed on the slide rod 103c.

[0046] The top of the heat-conducting ring 101b-1 contacts the bottom of the sample tube 102, and the first spring 101b provides elastic support for the sample tube 102. Without external force, the top of the sample tube 102 is kept exposed, which is convenient for personnel to remove the sample tube 102.

[0047] The fifth spring 103c-1 provides elastic support for the slide bar 103c, preventing it from moving downwards arbitrarily without external force.

[0048] The support plate 103a is slidably connected to the slide rod 103c. A second spring 103a-2 is fixed at the bottom of the support plate 103a. The other end of the second spring 103a-2 is fixed to the slide rod 103c. The pressure frame 103b is fixed to the slide rod 103c.

[0049] The second spring 103a-2 is sleeved on the slide rod 103c. The second spring 103a-2 can provide elastic support for the support plate 103a. When the slide rod 103c gradually moves down and the support plate 103a is not in contact with the sample holder 101, the slide rod 103c will move down and drive the support plate 103a to move at the same time. When the slide rod 103c gradually moves down and the support plate 103a contacts the sample holder 101, the support plate 103a can no longer move down and the second spring 103a-2 is stretched.

[0050] The fastening member 103a-3 includes a boss 103a-3a fixed to the top of the support plate 103a, a pressing block 103a-3b is provided on the top of the boss 103a-3a, and a third spring 103a-3c is fixed on the pressing block 103a-3b.

[0051] The pressure blocks 103a-3b are inclined, and the top of the pressure blocks 103a-3b is flat and in contact with the bottom of the pressure frame 103b.

[0052] The pressure frame 103b has a through hole with an inner diameter larger than that of the support groove 103a-1, in order to meet the space requirements for placing the sample tube 102.

[0053] The other end of the third spring 103a-3c is fixed to the top of the support plate 103a. The top of the boss 103a-3a is fixed with a guide block 103a-3d. The fastening member 103a-3 has a boss 103a-3a and cooperates with the guide block 103a-3d.

[0054] The third spring 103a-3c provides elastic support for the pressure block 103a-3b, preventing it from moving downwards arbitrarily when no external force is applied.

[0055] By setting the guide blocks 103a-3d and guide grooves 103a-3e, the positioning and guiding functions can be achieved, ensuring that the pressing block 103a-3b can be stably tilted downward and inward or stably tilted upward and outward.

[0056] In the initial state, the bottom end of the pressing block 103a-3b is misaligned with the support groove 103a-1, as shown in the attached diagram of the instruction manual. Figure 8 As shown, this will not affect the normal placement of sample tube 102.

[0057] The cover plate 301 has an installation groove 301a. The auxiliary heating plate 302 is fixed in the center of the installation groove 301a. The installation groove 301a has a first storage groove 301b on both sides, which cooperates with the auxiliary block 303c. The first storage groove 301b has a second storage groove 301c, which cooperates with the first storage groove 301b.

[0058] The installation slot 301a can meet the installation space requirements of the auxiliary heating plate 302. The first storage slot 301b can meet the lateral displacement space requirements of the auxiliary block 303c. The second storage slot 301c can meet the displacement space requirements of the first storage slot 301b, i.e., 301b-1.

[0059] The fixing plate 303a is located outside the auxiliary heating plate 302, the pressure rod 303a-2 corresponds to the sample tube 102, and the fixing plate 303a and the sliding plate 303a-1 are both provided with through grooves 303a-4.

[0060] With the setting of the pressure rod 303a-2, when the cover plate 301 is closed, the pressure rod 303a-2 will first contact the sample tube 102 and press the sample tube 102 into the test tube groove 101a.

[0061] By setting the through groove 303a-4, a small groove can be formed, which not only increases the exposed area of ​​the auxiliary heating plate 302, but also prevents operators from getting burned by inserting their fingers into the auxiliary heating plate 302.

[0062] A fourth spring 303c-1 is fixed on one side of the auxiliary block 303c, and the other end of the fourth spring 303c-1 is fixed in the first storage groove 301b. The end of the movable bar 303a-3 away from the slide plate 303a-1 is rotatably connected to the auxiliary block 303c.

[0063] The fourth spring 303c-1 provides elastic support for the auxiliary block 303c, preventing it from sliding and displacing arbitrarily without external force.

[0064] The top and bottom of the fixed plate 303a are provided with sliding grooves 303a-5, which cooperate with the sliding plate 303a-1.

[0065] By setting the slide groove 303a-5, the storage space requirements of the slide plate 303a-1 can be met, so that when the slide plate 303a-1 is stored, the area of ​​the protective component 303 blocking the auxiliary heating plate 302 is effectively reduced, and when the slide plate 303a-1 is unfolded, the area of ​​the protective component 303 blocking the auxiliary heating plate 302 is effectively increased.

[0066] The bottom of the sample holder 101 is in contact with the top of the heating plate 200. The bottom of the sample holder 101 is provided with a heat equalization hole 101c, which is offset from the test tube groove 101a.

[0067] By setting multiple heat equalization holes 101c, the bottom surface area of ​​the sample holder 101 can be increased, and the heat distribution on the sample holder 101 can be more uniform when the heating plate 200 is working.

[0068] The accompanying drawings are included in the instruction manual. Figure 3 The cross-sectional view of the sample holder 101 is formed by two sections, which is intended to facilitate understanding by those skilled in the art. The distribution of the test tube groove 101a and the heat spreader 101 is shown in the attached figure of the specification. Figure 4 As shown.

[0069] During use, all components are in the installed state. The placement component 100 is the sample placement area in the PCR gene amplification instrument, the heating plate 200 is the heating area in the PCR gene amplification instrument, and the slot cover component 300 is the heat cover in the PCR gene amplification instrument. When the slot cover component 300 is rotated and closed, it can fully cover the placement component 100. The working principle of this part is existing technology and will not be described in detail here. Those skilled in the art can clearly understand it.

[0070] When DNA samples need to be amplified, first place the sample tube 102 containing the corresponding DNA sample and sealed in the corresponding tube slot 101a. At this time, the protruding end of the sample tube 102 is placed in the support slot 103a-1. After placing multiple sample tubes 102, turn the cover plate 301 to close it, and then use the heating plate 200 to carry out subsequent amplification processing.

[0071] During the rotation and closing of the cover plate 301, the pressure rod 303a-2 first contacts the sample tube 102, pressing the sample tube 102 down, causing the heat-conducting ring 101b-1 to move down and compress the first spring 101b, while simultaneously driving the support plate 103a to slide on the slide rod 103c.

[0072] As the cover plate 301 rotates further to close, the cover plate 301 will come into contact with the slide bar 103c to press the slide bar 103c to move downward. The fifth spring 103c-1 is stretched, which drives the support plate 103a and the pressure frame 103b to move downward synchronously.

[0073] When the support plate 103a contacts the sample holder 101, the slide bar 103c moves further down, which stretches the second spring 103a-2, reduces the distance between the pressure frame 103b and the support plate 103a, and then the pressure frame 103b moves down to contact the pressing block 103a-3b, causing the pressing block 103a-3b to tilt downward and be placed into the support groove 103a-1. The third spring 103a-3c is compressed to press against the upper side of the sample tube 102 to ensure the stability of the sample tube 102.

[0074] Simultaneously, during the rotation and closing of the cover plate 301, the displacement block 303b-1 will also come into contact with the support plate 103a, causing the displacement frame 303b and the displacement block 303b-1 to retract into the second storage groove 301c, thereby squeezing the auxiliary block 303c to move outward into the first storage groove 301b. The fourth spring 303c-1 is compressed. Under the linkage of the movable bar 303a-3, the outward movement of the auxiliary block 303c allows the slide plate 303a-1 to slide in the slide groove 303a-5, so that the slide plate 303a-1 and the fixed plate 303a overlap, effectively reducing the area of ​​the slide plate 303a-1 and the fixed plate 303a obstructing the auxiliary heating plate 302. In conjunction with the through groove 303a-4, the top of the sample tube 102 can be subjected to auxiliary heat treatment when the auxiliary heating plate 302 is in working condition.

[0075] Similarly, when the cover plate 301 is rotated open, under the elastic support of the third spring 103a-3c, the pressing block 103a-3b resets and no longer obstructs the upward movement of the sample tube 102. Under the elastic support of the first spring 101b, the sample tube 102 automatically moves upward and protrudes. Under the elastic support of the second spring 103a-2, the support plate 103a moves upward and resets. Under the elastic support of the fifth spring 103c-1, the slide rod 103c and the pressure frame 103b move upward and reset. Under the elastic support of the fourth spring 303c-1, the auxiliary block 303c is displaced and reset, so that the displacement frame 303b is moved out of the second storage slot 301c. Under the action of the movable bar 303a-3, the two sliding plates 303a-1 slide and unfold.

[0076] In summary, when the cover plate 301 is closed, it can position and prevent the sample tube 102 from falling off. At the same time, the sliding plate 303a-1 retracts and shifts, and the auxiliary heating plate 302 can provide uniform auxiliary heating to the top of the sample tube 102. When the cover plate 301 is open, it can provide elastic support for the sample tube 102, which is convenient for personnel to remove the sample tube 102. At the same time, the sliding plate 303a-1 unfolds and shifts to shield the auxiliary heating plate 302, achieving the purpose of anti-scalding protection. Example 3

[0077] Reference Figures 2 to 13 This is the third embodiment of the present invention, which is based on the first two embodiments.

[0078] Specifically, thermally conductive silicone grease is filled between the bottom of the sample holder 101 and the heating plate 200 to fill the gaps between the sample holder 101 and the heating plate 200 caused by process limitations, thereby improving the overall uniformity of heat conduction.

[0079] The auxiliary block 303c has horizontal bars fixed at both the top and bottom, as shown in the attached diagram of the instruction manual. Figure 13 As shown in the attached diagram, the first storage slot 301b has a horizontal groove. Figure 9 As shown, the combination of horizontal bars and horizontal grooves can effectively improve the displacement stability of the auxiliary block 303c and prevent the auxiliary block 303c from detaching from the cover plate 301.

[0080] A protruding rod is fixed inside the second storage slot 301c, and the other end of the protruding rod passes through the displacement frame 303b to improve the stability of the displacement frame 303b during operation.

[0081] A sealing strip is embedded on the cover plate 301 and on the outside of the protective component 303 to prevent heat from leaking out when the cover plate is closed.

[0082] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered within the scope of the claims of the present invention.

Claims

1. A sample holder for a multifunctional PCR gene amplification instrument, characterized in that: include, The placement assembly (100) includes a sample holder (101) with a first spring (101b) disposed within it, a sample tube (102) disposed on top of the sample holder (101), and a stabilizer (103) disposed on top of the sample holder (101); and, A heating plate (200) is located at the bottom of the sample holder (101); and, The groove cover assembly (300) is located on top of the sample holder (101) and includes a cover plate (301) disposed outside the sample holder (101). An auxiliary heating plate (302) is fixed inside the cover plate (301). A protective component (303) is provided on the side of the auxiliary heating plate (302) away from the cover plate (301), including a fixing plate (303a) fixed on the cover plate (301). A sliding plate (303a-1) is slidably connected to the fixing plate (303a). A pressure rod (303a-2) is fixed on the fixing plate (303a). A movable strip (303a-3) is rotatably connected to the sliding plate (303a-1). A displacement frame (303b) is provided on the outside of the fixing plate (303a). A displacement block (303b-1) is fixed on the displacement frame (303b). An auxiliary block (303c) is provided on both sides of the fixing plate (303a).

2. The sample holder of the multifunctional PCR gene amplification instrument as described in claim 1, characterized in that: The stabilizer (103) includes a support plate (103a) located on top of the sample holder (101), a support groove (103a-1) is provided on the top of the support plate (103a), a fastening member (103a-3) is provided on the top of the support plate (103a), a pressure frame (103b) is provided on the top of the support plate (103a), and slide rods (103c) are slidably connected to the four corners of the sample holder (101). A fifth spring (103c-1) is sleeved on the slide rod (103c), the top end of the fifth spring (103c-1) is fixed on the sample holder (101), and the bottom end of the fifth spring (103c-1) is fixed on the slide rod (103c).

3. The sample holder of the multifunctional PCR gene amplification instrument as described in claim 2, characterized in that: The support plate (103a) is slidably connected to the slide rod (103c). A second spring (103a-2) is fixed at the bottom of the support plate (103a). The other end of the second spring (103a-2) is fixed to the slide rod (103c). The pressure frame (103b) is fixed to the slide rod (103c).

4. The sample holder of the multifunctional PCR gene amplification instrument as described in claim 3, characterized in that: The fastening member (103a-3) includes a boss (103a-3a) fixed to the top of the support plate (103a), a pressing block (103a-3b) is provided on the top of the boss (103a-3a), and a third spring (103a-3c) is fixed on the pressing block (103a-3b).

5. The sample holder of the multifunctional PCR gene amplification instrument as described in claim 4, characterized in that: The other end of the third spring (103a-3c) is fixed to the top of the support plate (103a), and a guide block (103a-3d) is fixed to the top of the boss (103a-3a). The fastening member (103a-3) has a boss (103a-3a) and cooperates with the guide block (103a-3d).

6. The sample holder of the multifunctional PCR gene amplification instrument as described in claim 1, characterized in that: The cover plate (301) has an installation groove (301a) and the auxiliary heating plate (302) is fixed in the center of the installation groove (301a). The installation groove (301a) has a first storage groove (301b) on both sides and cooperates with the auxiliary block (303c). The first storage groove (301b) has a second storage groove (301c) and cooperates with the first storage groove (301b).

7. The sample holder of the multifunctional PCR gene amplification instrument as described in claim 6, characterized in that: The fixing plate (303a) is located outside the auxiliary heating plate (302), the pressure rod (303a-2) corresponds to the sample tube (102), and the fixing plate (303a) and the sliding plate (303a-1) are both provided with through grooves (303a-4).

8. The sample holder of the multifunctional PCR gene amplification instrument as described in claim 7, characterized in that: A fourth spring (303c-1) is fixed on one side of the auxiliary block (303c), and the other end of the fourth spring (303c-1) is fixed in the first storage groove (301b). The end of the movable strip (303a-3) away from the slide plate (303a-1) is rotatably connected to the auxiliary block (303c).

9. The sample holder of the multifunctional PCR gene amplification instrument as described in claim 8, characterized in that: The fixed plate (303a) has grooves (303a-5) at both the top and bottom, which cooperate with the sliding plate (303a-1).

10. The sample holder of the multifunctional PCR gene amplification instrument as described in claim 1, characterized in that: The bottom of the sample holder (101) is in contact with the top of the heating plate (200). The bottom of the sample holder (101) is provided with a heat equalization hole (101c) and is offset from the test tube groove (101a). The test tube groove (101a) is provided in the sample holder (101) and cooperates with the sample test tube (102). The bottom end of the first spring (101b) is fixed in the test tube groove (101a), and the top end of the first spring (101b) is fixed with a heat-conducting ring (101b-1).

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