Microscope

Abstract

The invention discloses a microscope, and belongs to the technical field of illumination. The microscope comprises a light source module, a lens, a spectroscope and an objective lens, the light source module, the lens and the spectroscope are sequentially arranged, the objective lens is adjacent to the spectroscope, the light source module comprises a first light source and a second light source surrounding the first light source, and the objective lens is provided with a center channel and an edge channel located outside the center channel. Optical lenses are arranged in the annular channel and the edge channel, illumination modes of the microscope comprise a bright field illumination mode and a dark field illumination mode, the microscope is further provided with a sample bearing surface, and under the condition that the microscope is in the bright field illumination mode, only light emitted by the first light source enters the center channel after being transmitted by the lens and reflected by the spectroscope; the light is emitted to the sample bearing surface through the corresponding optical lens; when the microscope is in a dark field illumination mode, only the light emitted by the second light source enters the edge channel after being transmitted by the lens and reflected by the spectroscope, and is emitted to the sample bearing surface through the corresponding optical lens.

Description

Technical Field

[0001] This application belongs to the field of lighting technology, specifically relating to a microscope. Background Technology

[0002] Microscopes play an important role in scientific research and are widely used in fields such as cell science, immunology, and neuroscience.

[0003] Microscope illumination systems typically have multiple modes, including bright-field and dark-field illumination. Bright-field illumination involves directly illuminating the sample surface with a light source, causing it to reflect clear light and produce a bright effect. In bright-field illumination mode, the image field of view is clear and the colors are rich. Dark-field illumination, on the other hand, does not involve the light source directly illuminating the sample surface. Instead, a special illumination method is used to create shadows and reflected light on the sample surface. This causes uneven areas or defects on the sample surface to reflect light, creating a contrast between light and dark and highlighting the surface features of the sample. In dark-field illumination mode, the image outline is clear and the contrast is high.

[0004] In existing technologies, microscopes provide different light propagation paths for bright field illumination beams and dark field illumination beams. Different structures form different light propagation paths, resulting in complex internal structures, a large number of components, and high costs for microscopes. Summary of the Invention

[0005] The purpose of this application is to provide a microscope that can solve the problem of complex structure in microscopes in related technologies.

[0006] This application provides a microscope, including a light source module, a lens, a beam splitter, and an objective lens. The light source module, the lens, and the beam splitter are arranged sequentially, and the objective lens is arranged adjacent to the beam splitter. The light source module includes a first light source and a second light source, with the second light source surrounding the first light source. The objective lens has a central channel and an annular channel, with the annular channel surrounding the central channel. Optical lenses are disposed within both the annular channel and the central channel.

[0007] The microscope has two illumination modes: bright field and dark field. It also has a sample support surface.

[0008] When the microscope is in bright field illumination mode, only the light emitted by the first light source enters the central channel after being transmitted through the lens and reflected by the beam splitter, and is then projected onto the sample support surface through the corresponding optical lens;

[0009] When the microscope is in dark field illumination mode, only the light emitted by the second light source enters the annular channel after being transmitted through the lens and reflected by the beam splitter, and is then projected onto the sample support surface through the corresponding optical lens.

[0010] Optionally, the edge channel is an annular channel, which surrounds the central channel.

[0011] This setup allows almost all the light in dark field illumination mode to reach the sample support surface through the annular channel, which helps improve the dark field illumination effect.

[0012] Optionally, along the direction of the optical axis of the objective lens, the annular channel includes a cylindrical channel and a conical channel. The larger port of the conical channel is connected to the cylindrical channel, and the smaller port of the conical channel is opposite to the sample support surface. The optical lens in the annular channel includes an annular lens or a reflector. The annular lens or the reflector is located at the larger port of the conical channel. The light emitted by the second light source enters the cylindrical channel and is transmitted through the annular lens or reflected by the reflector before entering the conical channel.

[0013] This setup, by creating columnar channels, provides sufficient space for the central channel, avoiding interference with the light in bright-field illumination mode. Furthermore, it increases the angle at which light strikes the sample-bearing surface, preventing direct light from hitting the sample-bearing surface and improving the dark-field illumination effect.

[0014] Optionally, the microscope's illumination mode further includes a mixed illumination mode. The microscope also includes a control device, which is communicatively connected to the first light source and the second light source to control their emission states. When the microscope is in mixed illumination mode, the light emitted by the first light source enters the central channel after being transmitted through the lens and reflected by the beam splitter, and the light emitted by the second light source enters the edge channel after being transmitted through the lens and reflected by the beam splitter.

[0015] This setup, which uses a control device to control the light emission state of the first and second light sources, facilitates timely and accurate emission of both light sources. Furthermore, the addition of a mixed illumination mode to the microscope increases the variety of illumination modes, making it easier to use the microscope.

[0016] Optionally, the number of the first light source and the number of the second light source are both at least two, and the light-emitting area formed by the at least two first light sources is a first light-emitting area, which includes a plurality of first sub-light-emitting areas distributed in the direction surrounding the first light source; and / or, the light-emitting area formed by the at least two second light sources is a second light-emitting area, which includes a plurality of second sub-light-emitting areas distributed in the direction surrounding the second light source.

[0017] This configuration utilizes multiple first light sources to form multiple first sub-light-emitting areas, resulting in a larger amount of light directly hitting the sample support surface, which is beneficial for improving the bright-field illumination effect. Similarly, by utilizing multiple second light sources to form multiple second sub-light-emitting areas, a larger amount of light is obliquely incident on the sample support surface, which is beneficial for improving the dark-field illumination effect.

[0018] Optionally, the at least two first light sources are arranged in a ring array; and / or, the at least two second light sources are arranged in a ring array.

[0019] With this setup, the light emitted by the first and second light sources can form a circular illumination area with relatively uniform light intensity after hitting the sample support surface, which is beneficial for improving the bright field illumination effect and the dark field illumination effect.

[0020] Optionally, the microscope further includes a substrate located on the side of the lens facing away from the beam splitter, and the plurality of first light sources and the plurality of second light sources are all encapsulated on the surface of the substrate facing the lens.

[0021] With this configuration, each first light source and each second light source are encapsulated on the same surface of the substrate, which facilitates the installation of the first and second light sources. Moreover, it makes the distance between the first light source and the lens and the distance between the second light source and the lens equal, that is, the front working distance is equal, which is suitable for situations where the back focal plane of the objective lens is flush with the entrance end of the edge channel.

[0022] Optionally, the bright-field illumination mode includes a bright-field sheet illumination mode and a bright-field incident illumination mode, and the dark-field illumination mode includes a dark-field sheet illumination mode and a dark-field incident illumination mode. When the microscope is in the bright-field sheet illumination mode, a portion of the light emitted by the first light source corresponding to the first sub-emitting region enters the central channel after being transmitted through the lens and reflected by the beam splitter. When the microscope is in the bright-field incident illumination mode, all the light emitted by the first light source corresponding to the first sub-emitting region enters the central channel after being transmitted through the lens and reflected by the beam splitter. When the microscope is in the dark-field sheet illumination mode, a portion of the light emitted by the second light source corresponding to the second sub-emitting region enters the edge channel after being transmitted through the lens and reflected by the beam splitter. When the microscope is in the dark-field incident illumination mode, all the light emitted by the second light source corresponding to the second sub-emitting region enters the edge channel after being transmitted through the lens and reflected by the beam splitter.

[0023] This configuration, by controlling the emission of the first light source corresponding to part or all of the first sub-emitting regions, allows the microscope to be in bright-field sheet illumination mode or bright-field incident illumination mode, further increasing the variety of bright-field illumination modes; by controlling the emission of the second light source corresponding to part or all of the second sub-emitting regions, the microscope can be in dark-field sheet illumination mode or dark-field incident illumination mode, further increasing the variety of dark-field illumination modes. This is beneficial for expanding the microscope's functionality and making it more convenient to use.

[0024] Optionally, the microscope further includes a plurality of first controllers, each corresponding to a first sub-light-emitting region. Each first controller is communicatively connected to a first light source within its corresponding first sub-light-emitting region. Each first controller controls the light emission state of the first light source within its respective first sub-light-emitting region, so that the microscope is in the bright-field sheet illumination mode or the bright-field incident illumination mode. And / or, the microscope further includes a plurality of second controllers, each corresponding to a second sub-light-emitting region. Each first controller is communicatively connected to a second light source within its corresponding second sub-light-emitting region, and each second controller controls the light emission state of the second light source within its respective second sub-light-emitting region, so that the microscope is in the dark-field sheet illumination mode or the dark-field incident illumination mode.

[0025] This configuration, using multiple first controllers to control the luminescence state of the first light source in each first sub-luminescence region, facilitates the microscope to accurately and smoothly switch to bright-field slide illumination mode or bright-field incident illumination mode; using multiple second controllers to control the luminescence state of the second light source in each second sub-luminescence region, facilitates the microscope to accurately and smoothly switch to dark-field slide illumination mode or dark-field incident illumination mode.

[0026] Optionally, the beam splitter has a reflective surface facing the lens and the objective lens. The reflective surface includes a central region and an edge region, with the edge region surrounding the central region. The central region is provided with a beam-splitting film, and the edge region is provided with a reflective film. Light emitted by the first light source is reflected by the beam-splitting film, and light emitted by the second light source is reflected by the reflective film.

[0027] This configuration divides the beam splitter into sections, meaning that not the entire surface of the beam splitter is covered with a beam-splitting film. This allows only the light emitted from the first light source to pass through the beam-splitting film, while the light emitted from the second light source passes through the reflective film. This avoids the beams of the dark field illumination beam being split, which helps to improve the illumination effect in the dark field illumination mode.

[0028] Optionally, the spectral ratio of the spectral film is 1.

[0029] With this configuration and the specified splitting ratio, the beam splitter can both ensure that it has a certain splitting effect and reflect the light emitted by the first light source.

[0030] Optionally, the surface of the beam splitter facing away from the reflecting surface is provided with an anti-reflection coating.

[0031] This setup utilizes an anti-reflective coating to reduce light reflection from the imaging system and increase light transmittance, which helps improve imaging performance.

[0032] Optionally, the number of lenses is at least two, and each lens is spaced apart between the light source module and the beam splitter.

[0033] This setup utilizes the lens effect of at least two lenses to increase the refraction of light, ensuring that almost all light rays reach the beam splitter, which facilitates further light transmission through the objective lens and enhances the illumination effect.

[0034] Optionally, at least two of the lenses include a first lens and a second lens, and the light source module, the first lens, the second lens and the beam splitter are arranged in sequence. The microscope also includes an aperture stop and a field stop, with the aperture stop disposed between the light source module and the first lens, and the field stop disposed between the first lens and the second lens.

[0035] This configuration, by adding an aperture stop, allows control over the size of the light beam emitted from the light source module that passes through the first lens, limiting the amount of light and thus controlling its brightness. Furthermore, by adding a field stop, the imaging range of the sample can be limited, facilitating accurate observation and improving imaging quality.

[0036] Optionally, the microscope further includes a scattering plate disposed between the light source module and the aperture stop.

[0037] This setup, by adding a diffuser, makes the light entering the aperture stop more uniform, which helps to improve the lighting effect.

[0038] Optionally, the microscope further includes an imaging device located on the side of the beam splitter facing away from the objective lens. The microscope also includes a polarizer, an analyzer, and a differential interference prism. The polarizer is disposed between the light source module and the beam splitter, the analyzer is disposed between the imaging device and the beam splitter, and the differential interference prism is disposed between the beam splitter and the objective lens.

[0039] This setup helps to reduce the distance between the objective lens and the zoom lens, improving the vignetting effect; moreover, the combination of the polarizer, analyzer, and differential interference prism enables differential interference function, expanding the functionality of the microscope.

[0040] In this embodiment, the microscope is equipped with a light source module and a specially structured objective lens. The objective lens has a central channel for light in bright-field illumination mode and an edge channel for light in dark-field illumination mode. Thus, light from both illumination modes can pass through the objective lens. Furthermore, the light from both illumination modes is transmitted through the lens and reflected by the beam splitter. In other words, the light from both modes shares a common optical path before being reflected by the beam splitter. That is, the light from the bright-field illumination mode (emitted by the first light source) and the light from the dark-field illumination mode (emitted by the second light source) can sequentially pass through the lens and bevel the beam splitter before entering the corresponding channel within the objective lens and then reaching the sample-bearing surface. This configuration, with a shared optical path between bright-field and dark-field illumination modes, eliminates the need for complex structures to separately form light propagation paths for bright-field and dark-field illumination modes. This simplifies the microscope's structure, reduces the number of components, and lowers the microscope's cost. Attached Figure Description

[0041] Figure 1 This is a schematic diagram of the optical system of a microscope disclosed in an embodiment of this application;

[0042] Figure 2 This is a schematic diagram of the illumination system of a microscope disclosed in an embodiment of this application;

[0043] Figure 3 This is a schematic diagram of the imaging system of a microscope disclosed in an embodiment of this application;

[0044] Figure 4 This is a schematic diagram of the optical path of a microscope in bright field illumination mode, as disclosed in an embodiment of this application;

[0045] Figure 5 This is a schematic diagram of the optical path of a microscope in dark field illumination mode, as disclosed in an embodiment of this application;

[0046] Figure 6 This is a schematic diagram of the optical path of a microscope in mixed illumination mode, as disclosed in an embodiment of this application;

[0047] Figure 7 This is a schematic diagram of the structure of a light source module disclosed in an embodiment of this application;

[0048] Figure 8 This is a schematic diagram of a light source module under bright field illumination mode disclosed in an embodiment of this application;

[0049] Figure 9 This is a schematic diagram of a light source module in dark field incident illumination mode disclosed in an embodiment of this application;

[0050] Figures 10-13 This is a schematic diagram of a light source module under different bright field sheet illumination modes disclosed in an embodiment of this application;

[0051] Figures 14-17 This is a schematic diagram of a light source module under different dark field sheet illumination modes disclosed in an embodiment of this application;

[0052] Figure 18 This is a schematic diagram of the structure of a light source module disclosed in another embodiment of this application;

[0053] Figure 19 This is a schematic diagram of the structure of a light source module disclosed in another embodiment of this application;

[0054] Figures 20-24 This is a schematic diagram of a light source module under different dark field sheet illumination modes disclosed in another embodiment of this application;

[0055] Figure 25 This is a schematic diagram of the reflective surface of the beam splitter disclosed in the embodiments of this application;

[0056] Figure 26 This is a schematic diagram of the objective lens structure disclosed in the embodiments of this application;

[0057] Figure 27 This is a schematic diagram of the optical system of a microscope disclosed in another embodiment of this application.

[0058] Explanation of reference numerals in the attached figures:

[0059] 100 - Light source module, 110 - First light-emitting area, 110a - First sub-light-emitting area, 111 - First light source, 120 - Second light-emitting area, 120a - Second sub-light-emitting area, 121 - Second light source

[0060] 200 - Lens, 210 - First lens, 220 - Second lens

[0061] 300 - Beam splitter, 310 - Beam splitter coating, 320 - Reflective coating, 330 - Anti-reflective coating,

[0062] 400 - Objective lens, 410 - Central channel, 420 - Circular channel, 421 - Columnar channel, 422 - Conical channel, 500 - Control device

[0063] 610 - Aperture stop, 620 - Field stop, 630 - Diffuser

[0064] 710 - Camera device, 720 - Zoom lens,

[0065] 810 - Differential interference prism, 820 - Polarizer, 830 - Analyzer

[0066] 900-Substrate,

[0067] a-Sample bearing surface. Detailed Implementation

[0068] The technical solutions of the embodiments of this application will be clearly described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this application. All other embodiments obtained by those skilled in the art based on the embodiments of this application are within the scope of protection of this application.

[0069] The terms "first," "second," etc., used in the specification and claims of this application are used to distinguish similar objects and not to describe a specific order or sequence. It should be understood that such use of data can be interchanged where appropriate so that embodiments of this application can be implemented in orders other than those illustrated or described herein, and the objects distinguished by "first," "second," etc., are generally of the same class and the number of objects is not limited; for example, a first object can be one or more. Furthermore, in the specification and claims, "and / or" indicates at least one of the connected objects, and the character " / " generally indicates that the preceding and following objects are in an "or" relationship.

[0070] The microscope provided in the embodiments of this application will be described in detail below with reference to the accompanying drawings, through specific examples and application scenarios.

[0071] Please refer to Figures 1-27 The microscope disclosed in this application includes a light source module 100, a lens 200, a beam splitter 300, and an objective lens 400. The light source module 100 provides light in both bright field and dark field illumination modes. The lens 200 refracts the light, allowing the light emitted by the light source module 100 to pass through the beam splitter 300. The beam splitter 300 decomposes and separates the light emitted by the light source module 100 for analysis and processing. In this application, the beam splitter 300 primarily decomposes and separates the light in bright field illumination mode. The microscope also has a sample-bearing surface a, which holds the sample. The objective lens 400 collects light, directing it onto the sample held on the sample-bearing surface a for easy observation by the user.

[0072] The light source module 100, lens 200, beam splitter 300, and objective lens 400 together constitute the microscope's illumination system. For example... Figure 2 As shown, the light source module 100, lens 200, and beam splitter 300 are arranged sequentially, with objective lens 400 adjacent to beam splitter 300. Lens 200 and objective lens 400 are located at different positions on the same side of beam splitter 300. In this way, the light emitted by light source module 100 can pass through objective lens 400 after being transmitted through lens 200 and reflected by beam splitter 300.

[0073] The light source module 100 includes a first light source 111 and a second light source 121, with the second light source 121 arranged around the first light source 111. Optionally, there can be one or more first light sources 111, with each first light source 111 located at the center of the light source module 100; there can be multiple second light sources 121, spaced apart in the direction surrounding the first light source 111, or the second light source 121 can be a strip light source directly surrounding the first light source 111. Further optionally, both the first light source 111 and the second light source 121 can be LEDs (Light-Emitting Diode Lights), or other electrically powered light-emitting elements can be used.

[0074] The objective lens 400 has a central channel 410 and an edge channel 420. The edge channel 420 is located outside the central channel 410, and optical lenses are provided inside both the edge channel 420 and the central channel 410. Specifically, the central channel 410 and its internal optical lenses constitute the structure of the objective lens 400 in the prior art, while the edge channel 420 and its internal optical lenses are newly added structures to the objective lens 400 in the prior art. Since the first light source 111 is located in the central region of the light source module 100, the light emitted by the first light source 111 is mainly reflected by the middle region of the beam splitter 300 after being transmitted through the lens 200, and then enters the central channel 410. Therefore, the central channel 410 is used for the passage of the light emitted by the first light source 111. Similarly, since the second light source 121 is arranged around the first light source 111 and is located in the edge region of the light source module 100, the light emitted by the second light source 121 is mainly reflected by the edge region of the beam splitter 300 after being transmitted through the lens 200, and then enters the edge channel 420. Therefore, the edge channel 420 is used for the passage of the light emitted by the second light source 121. The optical path between the light source module 100 and the beam splitter 300 is a common optical path for the first light source 111 and the second light source 121.

[0075] Microscope illumination modes include bright-field illumination mode and dark-field illumination mode. For example... Figure 4 As shown, when the microscope is in bright-field illumination mode, only the light emitted by the first light source 111 enters the central channel 410 after being transmitted through the lens 200 and reflected by the beam splitter 300, and then shines onto the sample-bearing surface a through the corresponding optical lens. In this way, the light emitted by the first light source 111 directly shines on the sample-bearing surface a, causing the sample to reflect clear light and present a bright effect, thus achieving bright-field illumination.

[0076] like Figure 5 As shown, when the microscope is in dark-field illumination mode, only the light emitted by the second light source 121 enters the edge channel 420 after being transmitted through the lens 200 and reflected by the beam splitter 300, and then shines onto the sample support surface a through the corresponding optical lens. Thus, the light emitted by the second light source 121 does not directly illuminate the sample surface, but rather illuminates the sample at an angle from one side, causing shadows and reflected light to be generated on the sample surface, thereby achieving dark-field illumination.

[0077] In this embodiment, the microscope is equipped with a light source module 100 and a specially structured objective lens 400, which has a central channel 410 for light to pass through in bright field illumination mode and an edge channel 420 for light to pass through in dark field illumination mode. Thus, light from different illumination modes can pass through the objective lens 400. Moreover, light from different illumination modes is transmitted through the lens 200 and reflected by the beam splitter 300. That is to say, the light from the two modes has a common light path before being reflected by the beam splitter 300. That is, the light from the bright field illumination mode (the light emitted by the first light source 111) and the light from the dark field illumination mode (the light emitted by the second light source 121) can be transmitted through the lens 200 and reflected by the beam splitter 300 in sequence and enter the corresponding channel in the objective lens 400, and then be directed to the sample bearing surface a.

[0078] With this setup, the light in bright field illumination mode and dark field illumination mode share a common optical path, eliminating the need for complex structures to separately form light propagation paths in bright field illumination mode and dark field illumination mode. This simplifies the microscope's structure, reduces the number of components, and lowers the microscope's cost.

[0079] In one optional embodiment, the edge channel 420 is an arc-shaped channel that surrounds a portion of the central channel. Optionally, the second light source 121 can be an arc-shaped light source composed of multiple strip light sources, or it can include multiple light sources distributed in an arc-shaped array.

[0080] In another embodiment, the edge channel 420 is an annular channel surrounding the central channel 410. Optionally, the second light source 121 can be an annular light source composed of multiple strip light sources, or it can include multiple light sources distributed in a ring array. With this configuration, the edge channel 420 surrounds the entire central channel 410. Therefore, after the light emitted by the second light source 121 is transmitted through the lens 200 and reflected by the edge region of the beam splitter 300, almost all the light can enter the edge channel 420 and then reach the sample support surface a, improving light utilization and enhancing the dark field illumination effect.

[0081] In one optional embodiment, along the direction of the optical axis of the objective lens 400, the annular channel includes only a conical channel 422. The larger port of the conical channel 422 faces the beam splitter 300, and the smaller port of the conical channel 422 faces the sample support surface a. Thus, the light emitted by the second light source 121, after being transmitted through the lens 200 and reflected by the beam splitter 300, enters the conical channel 422. Under the action of the optical lenses within the conical channel 422, the light changes its path and is obliquely incident on the sample support surface a.

[0082] In another embodiment, reference Figure 26The objective lens 400 shown has an annular channel along its optical axis, comprising a cylindrical channel 421 and a conical channel 422. The larger port of the conical channel 422 is connected to the cylindrical channel 421, while the smaller port of the conical channel 422 is opposite to the sample support surface a. Thus, the light emitted from the second light source 121, after being transmitted through the lens 200 and reflected by the beam splitter 300, passes sequentially through the cylindrical channel 421 and the conical channel 422, and finally obliquely strikes the sample support surface a.

[0083] Moreover, the optical lenses in the annular channel include annular lenses or reflectors. The annular lenses or reflectors are located at the connection between the cylindrical channel 421 and the conical channel 422, that is, the annular lenses or reflectors are located at the larger port of the conical channel 422. The light emitted by the second light source 121 enters the cylindrical channel 421 and enters the conical channel 422 after being transmitted by the annular lens or reflected by the reflector.

[0084] Specifically, the optical lens may include a ring lens located at the junction of the cylindrical channel 421 and the conical channel 422, and the ring lens is arranged around the central channel 410. The light emitted by the second light source 121 passes through the cylindrical channel 421 and changes its path under the refraction of the ring lens before entering the conical channel 422. Alternatively, the optical lens may include a reflector, which may be a ring structure and is arranged around the central channel 410. Alternatively, there may be multiple reflectors, which are spaced apart along the direction surrounding the central channel 410, so that the light emitted by the second light source 121 passes through the cylindrical channel 421 and changes its path under the reflection of the reflector before entering the conical channel 422.

[0085] In this embodiment, by setting the columnar channel 421, sufficient space is reserved for the central channel 410 to avoid affecting the light in the bright field illumination mode; moreover, it is beneficial to increase the tilt of the light hitting the sample bearing surface a, avoid the light directly hitting the sample bearing surface a, and improve the dark field illumination effect.

[0086] In addition, the objective lenses 400 with different magnifications need to ensure that the diameter of the cylindrical channel 421 is approximately equal, so that the light emitted by the second light source 121 can always enter the cylindrical channel 421.

[0087] In a further embodiment, the microscope's illumination mode also includes a mixed illumination mode, referencing... Figure 6As shown, when the microscope is in mixed illumination mode, that is, simultaneously in bright field and dark field illumination modes, the light emitted by the first light source 111 is transmitted through the lens 200 and reflected by the beam splitter 300 before entering the central channel 410, and the light emitted by the second light source 121 is transmitted through the lens 200 and reflected by the beam splitter 300 before entering the annular channel 420. Thus, the microscope adds a mixed illumination mode, increasing the variety of illumination modes and making the microscope easier to use.

[0088] In one optional embodiment, the user can manually control the first light source 111 to emit light or the second light source 121 to emit light, so that the microscope is in bright field illumination mode, dark field illumination mode or mixed illumination mode.

[0089] In another embodiment, the microscope further includes a control device 500, which is communicatively connected to the first light source 111 and the second light source 121, respectively, to control the emission state of the first light source 111 and the second light source 121, so that the microscope is in a bright field illumination mode, a dark field illumination mode, or a mixed illumination mode. Specifically, when the control device 500 controls only the first light source 111 to emit light, the microscope is in a bright field illumination mode; when the control device 500 controls only the second light source 121 to emit light, the microscope is in a dark field illumination mode; and when the control device 500 controls both the first light source 111 and the second light source 121 to emit light simultaneously, the microscope is in a mixed illumination mode.

[0090] Optionally, the control device 500 can be a microcontroller, PLC (Programmable Logic Controller), or other device controlled by a program algorithm.

[0091] In this embodiment, the control device 500 controls the light emission state of the first light source 111 and the second light source 121, which is beneficial for the first light source 111 and the second light source 121 to emit light in a timely and accurate manner.

[0092] In the scheme of this application, the number of first light sources 111 is at least two, and the light-emitting area formed by the at least two first light sources is a first light-emitting area 110. The first light-emitting area 110 includes a plurality of first sub-light-emitting areas 110a distributed in the direction surrounding the first light sources 111. Optionally, the first light-emitting area 110 is an annular light-emitting area or an arc-shaped light-emitting area, and each first sub-light-emitting area 110a can be an arc-shaped light-emitting area. The number of first light sources 111 in each first sub-light-emitting area 110a can be the same or different, and the first sub-light-emitting area 110a can include one or more first light sources 111.

[0093] In this embodiment, multiple first light sources 111 are used to form multiple first sub-light-emitting regions 110a, resulting in a larger amount of light directly hitting the sample-bearing surface, which is beneficial to improving the bright-field illumination effect. Moreover, the multiple first sub-light-emitting regions 110a can switch light emission, so that the bright-field illumination mode of the microscope has different states.

[0094] Of course, in other embodiments, the number of first light sources 111 may be only one, so that the first light-emitting area 110 formed includes only one first sub-light-emitting area 110a.

[0095] In a further embodiment, at least two first light sources 111 are arranged in an arc-shaped array; or, referring to... Figures 7-24 As shown, at least two first light sources 111 are arranged in a ring array. Compared with the previous embodiment, in the latter embodiment, the light emitted by the first light source 111 can form a circular illumination area with a relatively uniform amount of light after hitting the sample support surface, which is beneficial to improving the bright field illumination effect.

[0096] In an optional embodiment, the bright field illumination mode includes a bright field patch illumination mode and a bright field incident illumination mode. The bright field patch illumination mode refers to the state when the first light source 111 corresponding to a portion of the first sub-light-emitting area 110a emits light, and the bright field incident illumination mode refers to the state when the first light source 111 corresponding to all the first sub-light-emitting areas 110a emits light.

[0097] Specifically, when the microscope is in bright-field slide illumination mode, such as Figures 10-13 As shown, the light emitted by the first light source 111 corresponding to a portion of the first sub-emitting region 110a is transmitted through the lens 200 and reflected by the beam splitter 300 before entering the central channel 410, further directly illuminating the sample supported on the sample-bearing surface a. Optionally, "a portion of the first sub-emitting region 110a" can refer to one first sub-emitting region 110a, or two or three adjacent first sub-emitting regions 110a, as long as it is a part of the total first sub-emitting regions 110a. When the microscope is in bright-field incident illumination mode, such as Figure 8 As shown, the light emitted by the first light source 111 corresponding to all the first sub-light emission regions 110a is transmitted through the lens 200 and reflected by the beam splitter 300 before entering the central channel 410 and further directly shining on the sample carried on the sample carrying surface a.

[0098] By using this embodiment, by controlling the emission of the first light source 111 corresponding to part or all of the first sub-light emission area 110a, the microscope is put into a bright field sheet illumination mode or a bright field incident illumination mode, which further increases the types of bright field illumination modes, which is beneficial to expanding the function of the microscope and making it more convenient to use the microscope.

[0099] In a further embodiment, the microscope also includes multiple first controllers, each corresponding to a first sub-light-emitting region 110a. Each first controller is communicatively connected to a first light source 111 within its corresponding first sub-light-emitting region 110a. Each first controller controls the illumination state of the first light source 111 within its respective first sub-light-emitting region 110a, thereby enabling the microscope to operate in either bright-field sheet illumination mode or bright-field direct illumination mode. Thus, each first sub-light-emitting region 110a is controlled by a separate first controller, achieving regional control of the first light-emitting region 110a. Specifically, as... Figures 10-13 As shown, different first controllers can be used to control the first light source 111 corresponding to different first sub-light emission regions 110a to emit light, thereby putting the microscope in different bright field sheet illumination modes.

[0100] In this embodiment, multiple first controllers are used to control the luminous state of the first light source 111 in each first sub-luminous region 110a, which is beneficial for the microscope to accurately and smoothly switch to bright field sheet illumination mode or bright field incident illumination mode.

[0101] Of course, in other embodiments, the luminous state of the first light source 111 in each first sub-luminous region 110a can be controlled by a manual control switch so that the microscope is in bright field sheet illumination mode or bright field incident illumination mode.

[0102] In this application, the number of second light sources 121 is also at least two, and the light-emitting area formed by at least two second light sources 121 is a second light-emitting area 120. The second light-emitting area 120 includes a plurality of second sub-light-emitting areas 120a distributed in the direction surrounding the second light sources 121. Optionally, the second light-emitting area 120 is an annular light-emitting area or an arc-shaped light-emitting area, and each second sub-light-emitting area 120a can be an arc-shaped light-emitting area. The number of second light sources 121 in each second sub-light-emitting area 120a can be the same or different, and the second sub-light-emitting area 120a can include one or more second light sources 121.

[0103] In this embodiment, multiple second light sources 121 are used to form multiple second sub-light-emitting regions 120a, resulting in a larger amount of light incident obliquely onto the sample-supporting surface, which is beneficial for improving the dark-field illumination effect. Moreover, the multiple second sub-light-emitting regions 120a can switch light emission, allowing the microscope's dark-field illumination mode to have different states.

[0104] Of course, in other embodiments, the number of second light sources 121 may be only one, so that the second light-emitting area 120 formed includes only one second sub-light-emitting area 120a.

[0105] In a further embodiment, at least two second light sources 121 are arranged in an arc-shaped array; or, referring to... Figures 7-24 As shown, at least two second light sources 121 are arranged in a ring array. Compared with the previous embodiment, in the latter embodiment, the light emitted by the second light source 121 can form a circular illumination area with a more uniform amount of light after hitting the sample bearing surface a, which is beneficial to improving the dark field illumination effect.

[0106] In an optional embodiment, the dark field illumination mode includes a dark field sheet illumination mode and a dark field incident illumination mode. The dark field sheet illumination mode refers to the state when the second light source 121 of a portion of the second sub-light-emitting region 120a emits light, and the dark field incident illumination mode refers to the state when the second light source 121 of all the second sub-light-emitting regions 120a emits light.

[0107] Specifically, when the microscope is in dark-field slide illumination mode, such as Figures 14-17 As shown, the light emitted by the second light source 121 corresponding to a portion of the second sub-emitting region 120a is transmitted through the lens 200 and reflected by the beam splitter 300 before entering the edge channel 420 and further projecting onto the sample supported on the sample support surface a. Optionally, "a portion of the second sub-emitting region 120a" can refer to a single second sub-emitting region 120a, or two or three adjacent second sub-emitting regions 120a, as long as it is a part of the total second sub-emitting regions 120a. When the microscope is in dark-field epi-illumination mode, such as... Figure 9 As shown, all the second light sources 121 emit light, and the light emitted by the second light sources 121 corresponding to all the second sub-light emission areas 120a is transmitted through the lens 200 and reflected by the beam splitter 300 and enters the edge channel 420, and is further directed to the sample carried on the sample carrying surface a.

[0108] By using this embodiment, by controlling the emission of the second light source 121 corresponding to part or all of the second sub-light emission region 120a, the microscope is put into dark field sheet illumination mode or dark field incident illumination mode, which further increases the types of dark field illumination modes, which is beneficial to expanding the function of the microscope and making it more convenient to use the microscope.

[0109] In a further embodiment, the microscope also includes multiple second controllers, each corresponding to a second sub-light-emitting region 120a. Each second controller is communicatively connected to a second light source 121 within its corresponding second sub-light-emitting region 120a. Each second controller controls the illumination state of the second light source 121 within its respective second sub-light-emitting region 120a, thereby enabling the microscope to operate in either dark-field slide illumination mode or dark-field direct illumination mode. Thus, each second sub-light-emitting region 120a is controlled by a separate second controller, achieving regional control of the second light-emitting region 120a. Specifically, as... Figures 14-17As shown, different second controllers can be used to control the second light source 121 corresponding to different second sub-light emission regions 120a to emit light, thereby putting the microscope in different dark field slide illumination modes.

[0110] Thus, by using multiple second controllers to control the luminescence state of the second light source 121 in each second sub-luminescence region 120a, it is beneficial for the microscope to accurately and smoothly switch to dark field slide illumination mode or dark field incident illumination mode.

[0111] Of course, in other embodiments, the luminescence state of the second light source 121 in each second sub-luminescence region 120a can be controlled by a manual control switch so that the microscope is in dark field sheet illumination mode or dark field incident illumination mode.

[0112] In summary, multiple first controllers and multiple second controllers together constitute the control device 500 mentioned above. Therefore, by controlling the light emission state of each first light source 111 and each second light source 121 through multiple first controllers and multiple second controllers, the microscope can switch between several illumination modes, including bright field slide illumination mode, bright field incident illumination mode, dark field slide illumination mode, dark field incident illumination mode, and mixed illumination mode. This effectively increases the variety of illumination modes, and users can switch to the required illumination mode according to the needs of observing the sample, which is beneficial for convenient use of the microscope.

[0113] In an optional embodiment, the first light source 111 and the second light source 121 may be located in the same plane, specifically, as shown below. Figures 1-2 as well as Figures 4-5 As shown, the microscope also includes a substrate 900, which is located on the side of the lens 200 facing away from the beam splitter 300. Multiple first light sources 111 and multiple second light sources 121 are encapsulated on the surface of the substrate 900 facing the lens 200. Optionally, the substrate 900 can be a plate-like structure with a supporting effect, such as a glass plate or steel sheet. This application does not limit the specific structure of the substrate 900, as long as the surface of the substrate 900 facing the lens 200 is planar and can be used to encapsulate multiple first light sources 111 and multiple second light sources 121.

[0114] In this embodiment, each first light source 111 and each second light source 121 are packaged on the same surface of the substrate 900, which facilitates the installation of the first light source 111 and the second light source 121. Moreover, the distance between the first light source 111 and the lens 200 and the distance between the second light source 121 and the lens 200 are equal, that is, the front working distance is equal, which is suitable for situations where the back focal plane of the objective lens 400 is flush with the entrance end of the edge channel 420.

[0115] Of course, in other embodiments, the first light source 111 and the second light source 121 may also be located in different planes. Specifically, the distance between the light source module 100 and the lens 200 is the front working distance, and the distance between the lens 200 and the back focal plane of the objective lens 400 or the entrance end of the edge channel 420 is the back working distance. When there is a height difference between the back focal plane of the objective lens 400 and the entrance end of the edge channel 420, it indicates that the back working distance in the bright field illumination mode is not equal to the back working distance in the dark field illumination mode. Therefore, the front working distance in the bright field illumination mode and the front working distance in the dark field illumination mode can also be set to be unequal, that is, the distances from the first light source 111 and the second light source 121 to the lens 200 are not equal, and the first light source 111 and the second light source 121 are located in different planes. Here, the back focal plane refers to the position where the light emitted by the first light source 111 is imaged in the objective lens 400 after being transmitted through the lens 200 and reflected by the beam splitter 300.

[0116] Optionally, at least two substrates 900 can be provided, with different substrates 900 having different distances from the lens 200. In this way, the first light source 111 and the second light source 121 can be encapsulated using different substrates 900.

[0117] In one optional embodiment, the first light source 111 and the second light source 121 can be LED lights, and a plurality of second light sources 121 are arranged around the first light source 111. Optionally, as... Figure 19 As shown, four second light sources 121 are arranged around the first light source 111, each second light source 121 forming a second sub-light-emitting region 120a, for a total of four second sub-light-emitting regions 120a; or, as... Figure 7 As shown, eight second light sources 121 are provided around the first light source 111. Each pair of adjacent second light sources 121 forms a second sub-light-emitting region 120a, forming a total of four second sub-light-emitting regions 120a. Of course, other numbers of second light sources 121 can also be provided around the first light source 111, or three or other numbers of adjacent second light sources 121 can form a second sub-light-emitting region 120a.

[0118] Multiple first light sources 111 are arranged within the second light-emitting region 120 formed by multiple second light sources 121, wherein some of the first light sources 111 are arranged in a ring array. When the microscope is in bright-field sheet illumination mode, a portion of the multiple first light sources 111 arranged in the ring array emits light. Optionally, as... Figure 19 As shown, there are five first light sources 111, four of which are arranged in a circular array and surround another first light source 111. Further, optionally, as... Figure 20As shown, each first light source 111 forms a first sub-emitting region 110a. When the microscope is in bright-field sheet illumination mode, one of the multiple first light sources 111 arranged in a ring array emits light; or, referring to... Figures 21-24 As shown, two adjacent first light sources 111 form a first sub-light emission region 110a. When the microscope is in bright field sheet illumination mode, two adjacent first light sources 111 arranged in a ring array emit light.

[0119] Optionally, such as Figures 7-17 As shown, there can be nine first light sources 111, with eight of them arranged in a circular array and surrounding another first light source 111. In the multiple first light sources 111 arranged in a circular array, each pair of adjacent first light sources 111 forms a first sub-light-emitting region 110a. Of course, the number of first light sources 111 can also be set to other numbers, or one first light source 111 or other number of first light sources 111 can form a first sub-light-emitting region 110a. In short, the first light sources 111 are arranged in a circular array, and the second light sources 121 are arranged in a circular array.

[0120] In another embodiment, such as Figure 18 As shown, the first light source 111 and the second light source 121 can be fiber optic groups, i.e., the first light source 111 is a first fiber optic group, and the second light source 121 is a second fiber optic group. Each fiber optic group is coupled by a separate light source. Multiple second fiber optic groups are arranged outside the first light source 111, and each second fiber optic group serves as a second sub-light-emitting region 120a. The number of second fiber optic groups can be four. Of course, other numbers of second fiber optic groups can also be arranged around the first light source 111. Multiple first fiber optic groups are arranged within the second light-emitting region 120 formed by multiple second fiber optic groups. Optionally, the number of first fiber optic groups can be five, with four first fiber optic groups arranged in a ring array and surrounding another first fiber optic group. Each first fiber optic group serves as a first sub-light-emitting region 110a. When the microscope is in bright-field sheet illumination mode, a portion of the four first fiber optic groups in the ring array emits light, i.e., one first fiber optic group emits light, or two or three adjacent first fiber optic groups emit light. When the microscope is in bright-field incident illumination mode, all first fiber optic groups emit light. Of course, other numbers of first fiber optic groups can also be arranged.

[0121] In one optional embodiment, the beam splitter 300 has a reflective surface facing the lens 200 and the objective lens 400, and the entire reflective surface is provided with a beam splitting film 310.

[0122] In another embodiment, the reflective surface includes a central region and an edge region, the edge region surrounding the central region, such as... Figure 25As shown, a beam splitter 310 is provided in the central region. The beam splitter 310 mainly decomposes and separates light, so that the light rays that hit the film layer are reflected and projected in a certain proportion. A reflective film 320 is provided in the edge region. The light rays that hit the reflective film 320 mainly perform the function of reflection. The light rays emitted by the first light source 111 are reflected by the beam splitter 310, and the light rays emitted by the second light source 121 are reflected by the reflective film 320.

[0123] The shape of the beam splitter 310 is similar to the shape of the light spot formed on the beam splitter 300 by the light emitted from the first light source 111. Optionally, the first light source 111 is arranged in a ring, and the first light source 111 forms an elliptical light spot on the beam splitter 300 after being transmitted through the lens 200. Therefore, the beam splitter 310 can be an elliptical structure. Of course, the beam splitter 310 can also be other shapes.

[0124] In this embodiment, the area of ​​the beam splitter 300 is divided, that is, not the entire surface of the beam splitter 300 is provided with the beam splitting film 310, so that only the light emitted by the first light source 111 passes through the beam splitting film 310, while the light emitted by the second light source 121 passes through the reflective film 320, thus avoiding the splitting of the dark field illumination beam and improving the illumination effect in the dark field illumination mode.

[0125] Optionally, the beam splitter 310 has a splitting ratio of 1. This setting ensures that the beam splitter has a certain splitting effect while also allowing it to reflect the light emitted by the first light source.

[0126] Of course, the spectral ratio of the spectral separator 310 can be other values.

[0127] In optional embodiments, such as Figure 6 As shown, the surface of the beam splitter 300 facing away from the reflective surface is provided with an anti-reflection coating 330, that is, the surface of the beam splitter 300 facing the camera device 710 is provided with an anti-reflection coating 330.

[0128] In this embodiment, the antireflective coating 330 is used to reduce the light reflection of the imaging system and increase the light transmittance, which is beneficial to improving the imaging effect.

[0129] Of course, in other embodiments, the surface of the beam splitter 300 facing away from the reflective surface may not be provided with an anti-reflection coating 330.

[0130] In one alternative embodiment, the number of lenses 200 is one, which is located between the light source module 100 and the beam splitter 300.

[0131] In another embodiment, at least two lenses 200 are used, with each lens 200 spaced apart between the light source module 100 and the beam splitter 300. In this embodiment, the light emitted from the light source module 100 passes sequentially through at least two lenses 200, increasing the refraction effect and ensuring that almost all light reaches the beam splitter 300. This facilitates further light transmission through the objective lens 400, improving the illumination effect.

[0132] In a further embodiment, at least two lenses 200 include a first lens 210 and a second lens 220, and the light source module 100, the first lens 210, the second lens 220, and the beam splitter 300 are arranged sequentially; as shown Figure 2 As shown, the microscope also includes an aperture stop 610 and a field stop 620. The aperture stop 610 is positioned between the light source module 100 and the first lens 210, and the field stop 620 is positioned between the first lens 210 and the second lens 220. Thus, the light emitted from the light source module 100 passes sequentially through the field stop 620, the first lens 210, the aperture stop 610, and the second lens 220 before reaching the beam splitter 300.

[0133] In this embodiment, by adding an aperture stop 610, the size of the light beam emitted by the light source module 100 that passes through the first lens 210 can be controlled, and the amount of light can be limited to control the brightness of the light. By adding a field stop 620, the range of sample imaging can be limited, which is beneficial for accurately observing the sample and improving the imaging effect.

[0134] In optional embodiments, such as Figure 2 As shown, the microscope also includes a diffuser 630, which is disposed between the light source module 100 and the aperture stop 610. Thus, the light emitted from the light source module 100 is diffused by the diffuser 630 before passing through the aperture stop 610. In this embodiment, by adding the diffuser 630, the light entering the aperture stop 610 is more uniform, which helps to improve the illumination effect.

[0135] Of course, in other embodiments, the microscope may not have a diffuser 630, and the light emitted by the light source module 100 may be directly incident on the first lens 210.

[0136] In an optional embodiment, refer to Figure 1 and Figure 3As shown, the microscope also includes an imaging device 710, which is located on the side of the beam splitter 300 facing away from the objective lens 400. Optionally, the imaging device 710 can be a camera or other structure capable of imaging. The microscope also includes a zoom lens 720, which is located between the beam splitter 300 and the imaging device 710. Thus, the dark-field illumination source (i.e., the second light source 121) does not need to be positioned between the beam splitter 300 and the objective lens 400. Therefore, no space needs to be reserved between the beam splitter 300 and the objective lens 400 for installing the dark-field illumination source, allowing the zoom lens 720, beam splitter 300, and objective lens 400 to be arranged more compactly, reducing the distance between the objective lens 400 and the zoom lens 720, which is beneficial for improving vignetting effects.

[0137] In a further embodiment, reference is made to... Figure 27 As shown, the microscope also includes a polarizer 820, an analyzer 830, and a differential interference prism 810. The polarizer 820 is positioned between the light source module 100 and the beam splitter 300; the analyzer 830 is positioned between the imaging device 710 and the beam splitter 300; and the differential interference prism 810 is positioned between the beam splitter 300 and the objective lens 400. Optionally, the polarizer 820 can be located between the beam splitter 300 and the first lens 210, between the first lens 210 and the second lens 220, or between the second lens 220 and the beam splitter 300. In short, the polarizer 820 only needs to be located between the light source module 100 and the beam splitter 300. The analyzer 830 can be located between the imaging device 710 and the zoom lens 720, or between the zoom lens 720 and the beam splitter 300.

[0138] The polarizer 820 is mainly used to obtain polarized light from the light source, while the analyzer 830 is used to analyze and detect the polarization state of the light. The combination of the polarizer 820, analyzer 830, and differential interference prism 810 enables differential interference, thus expanding the functionality of the microscope. Moreover, because the dark field illumination source is not positioned between the beam splitter 300 and the objective lens 400, the differential interference prism 810 can be placed between them to achieve differential interference.

[0139] Of course, in other embodiments, the microscope may not be equipped with the polarizer 820, analyzer 830 and differential interference prism 810, that is, the microscope does not have differential interference function.

[0140] The embodiments of this application have been described above with reference to the accompanying drawings. However, this application is not limited to the specific embodiments described above. The specific embodiments described above are merely illustrative and not restrictive. Those skilled in the art can make many other forms under the guidance of this application without departing from the spirit and scope of the claims, and all of these forms are within the protection scope of this application.

Claims

1. A microscope, characterized in that, The system includes a light source module (100), a lens (200), a beam splitter (300), and an objective lens (400). The light source module (100), the lens (200), and the beam splitter (300) are arranged sequentially. The objective lens (400) is arranged adjacent to the beam splitter (300). The light source module (100) includes a first light source (111) and a second light source (121). The second light source (121) is arranged around the first light source (111). The objective lens (400) has a central channel (410) and an edge channel (420). The edge channel (420) is located outside the central channel (410), and optical lenses are provided inside both the edge channel (420) and the central channel (410). The microscope has two illumination modes: a bright field illumination mode and a dark field illumination mode. The microscope also has a sample support surface (a). When the microscope is in bright field illumination mode, only the light emitted by the first light source (111) enters the central channel (410) after being transmitted through the lens (200) and reflected by the beam splitter (300), and is then projected onto the sample support surface (a) through the corresponding optical lens; When the microscope is in dark field illumination mode, only the light emitted by the second light source (121) enters the edge channel (420) after being transmitted through the lens (200) and reflected by the beam splitter (300), and is then projected onto the sample support surface (a) through the corresponding optical lens.

2. The microscope according to claim 1, characterized in that, The edge channel (420) is a ring channel, which surrounds the central channel (410).

3. The microscope according to claim 2, characterized in that, Along the direction of the optical axis of the objective lens (400), the annular channel includes a cylindrical channel (421) and a conical channel (422). The larger port of the conical channel (422) is connected to the cylindrical channel (421), and the smaller port of the conical channel (422) is opposite to the sample support surface (a). The optical lens in the annular channel includes an annular lens or a reflector. The annular lens or the reflector is located at the larger port of the conical channel (422). The light emitted by the second light source (121) enters the columnar channel (421) and is transmitted through the annular lens or reflected by the reflector before entering the conical channel (422).

4. The microscope according to claim 1, characterized in that, The microscope's illumination mode also includes a mixed illumination mode. The microscope further includes a control device (500) which is communicatively connected to the first light source (111) and the second light source (121) to control the light emission states of the first light source (111) and the second light source (121). When the microscope is in mixed illumination mode, the light emitted by the first light source (111) is transmitted through the lens (200) and reflected by the beam splitter (300) and enters the central channel (410), and the light emitted by the second light source (121) is transmitted through the lens (200) and reflected by the beam splitter (300) and enters the edge channel (420).

5. The microscope according to claim 1, characterized in that, The number of the first light source (111) and the second light source (121) is at least two. The light-emitting area formed by the at least two first light sources (111) is a first light-emitting area (110). The first light-emitting area (110) includes a plurality of first sub-light-emitting areas (110a) distributed in the direction surrounding the first light source (111). The light-emitting area formed by the at least two second light sources (121) is a second light-emitting area (120), which includes a plurality of second sub-light-emitting areas (120a) distributed in a direction surrounding the second light source (121).

6. The microscope according to claim 5, characterized in that, The at least two first light sources (111) are arranged in a ring array; And / or, the at least two second light sources (121) are arranged in a ring array.

7. The microscope according to claim 5, characterized in that, The microscope also includes a substrate (900) located on the side of the lens (200) facing away from the beam splitter (300), and the plurality of first light sources (111) and the plurality of second light sources (121) are all encapsulated on the surface of the substrate (900) facing the lens (200).

8. The microscope according to claim 5, characterized in that, The brightfield illumination mode includes brightfield sheet illumination mode and brightfield incident illumination mode, and the darkfield illumination mode includes darkfield sheet illumination mode and darkfield incident illumination mode. When the microscope is in the bright field illumination mode, the light emitted by the first light source (111) corresponding to a portion of the first sub-light emission area (110a) enters the central channel (410) after being transmitted through the lens (200) and reflected by the beam splitter (300). When the microscope is in the bright field illumination mode, the light emitted by the first light source (111) corresponding to all the first sub-light emission areas (110a) enters the central channel (410) after being transmitted through the lens (200) and reflected by the beam splitter (300). When the microscope is in the dark field sheet illumination mode, the light emitted by the second light source (121) corresponding to a portion of the second sub-light emission area (120a) enters the edge channel (420) after being transmitted through the lens (200) and reflected by the beam splitter (300); When the microscope is in the dark field incident illumination mode, the light emitted by the second light source (121) corresponding to all the second sub-light emission areas (120a) enters the edge channel (420) after being transmitted through the lens (200) and reflected by the beam splitter (300).

9. The microscope according to claim 8, characterized in that, The microscope also includes a plurality of first controllers, each of which corresponds to a first sub-light emission area (110a). Each first controller is communicatively connected to the first light source (111) in the corresponding first sub-light emission area (110a). Each first controller controls the light emission state of the first light source (111) in each first sub-light emission area (110a) so that the microscope is in the bright field sheet illumination mode or the bright field incident illumination mode. And / or, the microscope further includes a plurality of second controllers, each of which corresponds one-to-one with a second sub-luminescent region (120a). Each second controller is communicatively connected to a second light source (121) within the corresponding second sub-luminescent region (120a). Each second controller controls the luminescence state of the second light source (121) within each second sub-luminescent region (120a) to enable the microscope to be in the dark field sheet illumination mode or the dark field incident illumination mode.

10. The microscope according to claim 1, characterized in that, The beam splitter (300) has a reflective surface facing the lens (200) and the objective lens (400). The reflective surface includes a central region and an edge region, with the edge region surrounding the central region. The central region is provided with a beam splitter film (310), and the edge region is provided with a reflective film (320). Light emitted by the first light source (111) is reflected by the beam splitter film (310), and light emitted by the second light source (121) is reflected by the reflective film (320).

11. The microscope according to claim 10, characterized in that, The spectral ratio of the spectral film (310) is 1.

12. The microscope according to claim 10, characterized in that, The surface of the beam splitter (300) facing away from the reflecting surface is provided with an anti-reflection coating (330).

13. The microscope according to claim 1, characterized in that, The number of lenses (200) is at least two, and each lens (200) is spaced apart between the light source module (100) and the beam splitter (300).

14. The microscope according to claim 13, characterized in that, At least two of the lenses (200) include a first lens (210) and a second lens (220), and the light source module (100), the first lens (210), the second lens (220), and the beam splitter (300) are arranged sequentially. The microscope also includes an aperture stop (610) and a field stop (620). The aperture stop (610) is disposed between the light source module (100) and the first lens (210), and the field stop (620) is disposed between the first lens (210) and the second lens (220).

15. The microscope according to claim 14, characterized in that, The microscope also includes a scattering plate (630), which is disposed between the light source module (100) and the aperture stop (610).

16. The microscope according to claim 1, characterized in that, The microscope also includes an imaging device (710) located on the side of the beam splitter (300) facing away from the objective lens (400). The microscope also includes a polarizer (820), an analyzer (830), and a differential interference prism (810). The polarizer (820) is disposed between the light source module (100) and the beam splitter (300). The analyzer (830) is disposed between the imaging device (710) and the beam splitter (300). The differential interference prism (810) is disposed between the beam splitter (300) and the objective lens (400).

Images