Method for dynamically regulating and controlling organic soil of Chinese rose through in-situ buffering

By constructing an in-situ buffer system and using dynamic regulation methods in rose organic soil, the problems of low pH regulation accuracy and short cycle were solved, achieving a long-term and stable synergistic effect of pH regulation and nutrient supply, promoting the healthy growth of roses and the efficient absorption of nutrients.

CN120959125APending Publication Date: 2025-11-18SHENZHEN TIMES HORTICULTURE FLORAL CO LTD
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Patent Information

Application Number
CN202511029865.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-07-25
Publication Date
2025-11-18

AI Technical Summary

Technical Problem

In existing technologies, the pH regulation of rose organic soil has low precision and short cycle, and pH regulation is disconnected from nutrient supply, resulting in root stress and low nutrient utilization.

Method used

By constructing an in-situ buffer system in organic soil, soluble calcium acetate is generated by reacting calcium carbonate with glacial acetic acid. This is combined with cation exchange enhancers such as biochar or vermiculite to form a multiphase buffer system. Functional regulators are then applied at different growth stages to achieve dynamic regulation.

Benefits of technology

It achieves long-term and stable pH regulation, reduces pH fluctuations, improves nutrient utilization, and promotes the healthy growth of roses and the absorption of nutrients.

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Abstract

The invention relates to the field of Chinese rose cultivation, and discloses a method for dynamically regulating and controlling organic soil of Chinese rose through in-situ buffering, which comprises the following steps: S1, pretreatment: mixing and disinfecting basic organic soil containing an organic carrier and a physical modifier; s2, in-situ buffer system construction: adding a solid-phase alkali storage and a buffer system generating agent into the basic organic soil, so that the solid-phase alkali storage and the buffer system generating agent are subjected to an in-situ reaction in the basic organic soil to generate an in-situ multi-phase buffer system composed of a liquid-phase buffer pair and the solid-phase alkali storage, and buffer type organic soil is obtained; s3, dynamic regulation and control: in different growth stages of the Chinese rose, applying a functional regulation and control agent into the buffer type organic soil so as to regulate the pH value of the buffer type organic soil and maintain the pH value within a preset target range corresponding to each growth stage. According to the method, the in-situ multiphase buffer system is constructed, and the functional regulating agent is applied, so that pH long-acting buffer and dynamic regulation and control coordinated with nutrition supply are realized.
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Description

TECHNICAL FIELD

[0001] The present application relates to the field of rose cultivation, in particular to a method for dynamically regulating the pH value of rose organic soil by in-situ buffering. BACKGROUND

[0002] As a plant with important ornamental and economic value, the healthy growth and flowering quality of rose largely depend on the physicochemical properties of the cultivation substrate. Among the many influencing factors, the pH value of the soil is a core parameter that directly affects the physiological activities of rose roots and the absorption efficiency of various essential nutrients. Therefore, developing a method that can accurately and long-acting regulate the pH value of rose organic soil is a technical problem of concern in the field of horticultural cultivation, especially in fine planting management.

[0003] In the prior art, the adjustment of the soil pH value for rose cultivation usually involves pre-adjustment by mixing alkaline materials such as lime and wood ash, or acidic materials such as sulfur powder into the basic cultivation substrate at one time. In the subsequent growth management, the operator will make a judgment based on experience and use some commercially available acidic or alkaline fertilizers and solutions for subsequent supplementary adjustment.

[0004] Although the existing technology provides certain means for adjusting the pH value of the soil, there are still some deficiencies in the application process. First, the direct reaction of a large amount of strong acid-base modifier added at one time with the soil easily leads to a dramatic and nonlinear change in the pH value, and even overcorrection, causing stress to the rose roots. This is because this method lacks an internal buffering mechanism to suppress this chemical shock, and its adjustment effect completely depends on the instantaneous reaction of the material. Second, these modifiers and their reaction products exist in the soil solution in the form of free ions, which are easily leached and lost with water under conventional irrigation, resulting in insufficient durability of the adjustment effect. The fundamental reason is that the cation exchange capacity of the traditional substrate is limited, which cannot effectively anchor these active ions, causing waste of materials and shortening of the regulation period. In addition, the pH adjustment process and nutrient supply process of the existing technology are usually separated, and fertilization under unsuitable pH conditions often leads to a decrease in the bioavailability of specific nutrients (such as phosphorus, iron, etc.) due to chemical precipitation or fixation, resulting in low nutrient utilization rate. SUMMARY

[0005] The purpose of the present application is to provide a method for dynamically regulating the pH value of rose organic soil by in-situ buffering, which solves the problems of low accuracy, short action period, and separation of pH adjustment and nutrient supply in the prior art.

[0006] To achieve the above purpose, the present application is implemented by the following technical solutions:

[0007] The application provides a method for dynamically regulating organic soil of Chinese rose by in-situ buffering, comprising the following steps:

[0008] S1, pretreatment:

[0009] Firstly, an organic carrier, a physical modifier and a cation exchange enhancer are obtained. The organic carrier is preferably rotten leaf soil, and the amount is 750-850 parts by weight; the physical modifier is preferably perlite, and the amount is 50-100 parts by weight; and the cation exchange enhancer is preferably biochar or vermiculite, and the amount is 50-100 parts by weight. The above components are put into a mixing device and mixed for 12-18 minutes to obtain uniformly mixed basic organic soil. Then, the basic organic soil is subjected to high-temperature sterilization treatment under saturated steam at 105-121 ℃ for 45-60 minutes to obtain clean initial environment.

[0010] S2, construction of in-situ buffering system:

[0011] Firstly, 4-8 parts by weight of solid-phase alkali reserve (preferably calcium carbonate powder) is uniformly added to the pretreated basic organic soil. Then, acetic acid working solution of buffering system generator (preferably glacial acetic acid) is prepared. The preparation method of the acetic acid working solution is as follows: 30-115 parts by weight of deionized water is weighed, stirred at a speed of 100-300 rpm, and 0.6-1.2 parts by weight of glacial acetic acid is slowly added during the process, and the acetic acid working solution with a volume concentration of 1.0% to 2.0% is obtained after uniform mixing.

[0012] The prepared acetic acid working solution is uniformly sprayed into the basic organic soil containing calcium carbonate powder. At this time, the glacial acetic acid reacts with the calcium carbonate in the organic soil in-situ (2CH3COOH+CaCO3→Ca(CH3COO)2+H2O+CO2) to generate soluble calcium acetate. The calcium acetate and the excess unreacted calcium carbonate powder together form an in-situ heterogeneous buffering system coexisting with a “liquid-phase buffering pair-solid-phase alkali reserve”. At the same time, the generated calcium ions (Ca 2+ ) are adsorbed and anchored by the high-capacity cation exchange sites of the cation exchange enhancer (biochar or vermiculite) added in step 1.

[0013] In order to completely form and stabilize the above system, a curing process is further included: the reacted organic soil is stacked and sealed with a film, and is placed at an ambient temperature of 15-28 ℃ for 50-75 hours. After the curing is completed, the buffering-type organic soil with long-term buffering capacity is obtained.

[0014] S1, dynamic regulation:

[0015] The step is specifically: judging the growth stage of the Chinese rose, and selecting the corresponding functional regulator.

[0016] During the vegetative growth period of the Chinese rose, the preset target pH range of the rhizosphere environment is 6.0-6.5. At this time, an acidic functional regulator is selected for application. The acidic functional regulator is preferably a citric acid-potassium dihydrogen phosphate compound solution.

[0017] During the bud bearing and flowering period of the Chinese rose, the preset target pH range of the rhizosphere environment is 6.5-6.8. At this time, an alkaline functional regulator is selected for application. The alkaline functional regulator is preferably a potassium humate solution.

[0018] The whole dynamic regulation process also includes a monitoring feedback mechanism: the pH value of the buffer type organic soil is measured regularly using a pH meter, and the measured value is compared with the preset target range of the current growth stage. If the absolute value of the deviation is greater than 0.2, then the amount or frequency of the next application of the functional regulator is adjusted according to the size and sign of the deviation.

[0019] In summary, the present application includes at least one of the following beneficial technical effects:

[0020] 1. The present application constructs a multi-phase buffer system composed of a liquid-phase buffer pair and a solid-phase alkali reservoir by in-situ reaction in the basic organic soil. The calcium carbonate as the solid-phase reservoir can continuously supplement the components of the liquid-phase buffer pair consumed by neutralizing acidic substances, thereby endowing the organic soil with strong and lasting pH buffering capacity, effectively avoiding the soil pH value fluctuations that may be caused by conventional adjustment methods, and realizing long-acting stability of the rhizosphere environment pH value.

[0021] 2. The present application adds a cation exchange enhancer (such as biochar or vermiculite) with high cation exchange capacity in the pretreatment step, so that the key cations (such as Ca 2+ ) generated by in-situ reaction can be effectively adsorbed and anchored on the surface of soil particles. This structure significantly reduces the leaching loss of active components due to watering, prolongs the effective action period of the in-situ multi-phase buffer system, and improves the overall utilization efficiency of the material.

[0022] 3. The present application establishes a synergistic mechanism of pH regulation and nutrient supply by applying specific functional regulators at different growth stages of the Chinese rose. For example, the acidic functional regulator applied during the vegetative growth period not only reduces the pH value, but also supplements phosphorus, potassium elements and activates trace elements through chelation; the alkaline functional regulator applied during the bud bearing and flowering period stabilizes the pH value, supplements potassium elements and stimulates root growth. This method ensures that the Chinese rose efficiently absorbs the core nutrients required at its specific stage in the most suitable pH environment, and realizes the integration of regulation and fertilization. DETAILED DESCRIPTION

[0023] The application will be further described in detail in connection with the following examples, comparative examples and test examples.

[0024] The sources and specifications of the main raw materials and reagents used in the following examples and comparative examples are as follows, and the reagents not specifically described are commercially available analytical pure or higher grade products.

[0025] Example 1:

[0026] S1, pretreatment:

[0027] First, 750 parts of rotten soil as an organic carrier, 50 parts of perlite as a physical modifier, and 50 parts of biochar as a cation exchange enhancer were weighed according to the weight parts.

[0028] The weighed components were placed in a blender and mixed for 12 minutes to obtain a uniformly mixed basic organic soil. Subsequently, the basic organic soil was subjected to high-temperature steam sterilization, with the sterilization temperature set to 105°C and maintained at this temperature for 45 minutes. After sterilization, it was naturally cooled to room temperature for standby use.

[0029] S2, in-situ buffer system construction:

[0030] To the basic organic soil pretreated in step S1, 4 parts by weight of calcium carbonate powder was added as a solid-phase alkali reservoir and mixed uniformly.

[0031] Prepare the acetic acid working solution: in a container with stirring device, add 30 parts by weight of deionized water, start the stirring device, stir at a speed of 100 rpm, slowly add 0.6 parts by weight of glacial acetic acid to the deionized water in this state. After all the glacial acetic acid is added, continue stirring for 5 minutes until the system is mixed uniformly to form a clear and transparent acetic acid working solution.

[0032] Spray the prepared acetic acid working solution uniformly into the basic organic soil containing calcium carbonate powder to trigger the in-situ reaction.

[0033] After the reaction is completed, the organic soil is stacked and sealed with a film, and is allowed to stand for 50 hours at an ambient temperature of 15°C for curing, so that the in-situ multi-phase buffer system is fully formed and stabilized, and finally the buffer-type organic soil for subsequent testing is obtained.

[0034] S3, dynamic regulation:

[0035] The buffer type organic soil prepared in this embodiment will be managed by the dynamic regulation method of the application in the subsequent Rosa chinensis potting test. That is, during the vegetative growth period of Rosa chinensis, the acid functional regulator is applied to maintain the pH value of the organic soil at 6.0-6.5; during the budding and flowering period, the alkaline functional regulator is applied to maintain the pH value of the organic soil at 6.5-6.8.

[0036] Example 2:

[0037] S1, pretreatment:

[0038] First, 800 parts of rotten leaf soil are weighed as an organic carrier, 75 parts of perlite are weighed as a physical modifier, and 75 parts of vermiculite are weighed as a cation exchange enhancer. The weighed components are placed in a blender and mixed for 15 minutes to obtain a uniformly mixed base organic soil. Subsequently, the base organic soil is subjected to high-temperature steam sterilization, the sterilization temperature is set to 113°C, and maintained at this temperature for 52 minutes, and then naturally cooled to room temperature for standby after sterilization.

[0039] S2, in-situ buffer system construction:

[0040] To the base organic soil pretreated in step S1, 6 parts by weight of calcium carbonate powder is added as a solid-phase alkali reservoir, and mixed uniformly.

[0041] Prepare an acetic acid working solution: in a container with a stirring device, add 72.5 parts by weight of deionized water, start the stirring device, and stir at a rate of 200 rpm. Slowly add 0.9 parts by weight of glacial acetic acid to the deionized water under this condition. After all the glacial acetic acid is added, continue stirring for 7 minutes until the system is uniformly mixed to form a clear and transparent acetic acid working solution.

[0042] Spray the prepared acetic acid working solution uniformly into the base organic soil containing calcium carbonate powder to trigger the in-situ reaction.

[0043] After the reaction is completed, the organic soil is stacked and sealed with a film, and is allowed to stand for 62.5 hours for curing at an ambient temperature of 21.5°C, and finally the buffer type organic soil for subsequent testing is obtained.

[0044] S3, dynamic regulation: the buffer type organic soil prepared in this embodiment will be managed by the same dynamic regulation method as in Example 1 in the subsequent Rosa chinensis potting test.

[0045] Example 3:

[0046] S1, pretreatment:

[0047] Firstly, 850 parts of rotten leaf soil as an organic carrier, 100 parts of perlite as a physical modifier, and 100 parts of biochar as a cation exchange enhancer were weighed. The weighed components were placed in a blender and mixed for 18 minutes to obtain a uniformly mixed base organic soil. Subsequently, the base organic soil was subjected to high-temperature steam sterilization, the sterilization temperature was set to 121°C, and maintained at this temperature for 60 minutes, and after sterilization, it was naturally cooled to room temperature for standby.

[0048] S2, in-situ buffer system construction:

[0049] To the base organic soil pretreated in step S1, 8 parts by weight of calcium carbonate powder was added as a solid-phase alkali reservoir, and mixed uniformly.

[0050] Separately prepare an acetic acid working solution: in a container with stirring device, add 115 parts by weight of deionized water, start the stirring device, stir at a rate of 300 rpm, slowly add 1.2 parts by weight of glacial acetic acid to the deionized water. After all the glacial acetic acid is added, continue to stir for 10 minutes until the system is uniformly mixed to form a clear and transparent acetic acid working solution.

[0051] The prepared acetic acid working solution was uniformly sprayed onto the base organic soil containing calcium carbonate powder to trigger the in-situ reaction.

[0052] After the reaction is completed, the organic soil is stacked and sealed with a film, and is allowed to stand for 75 hours at an ambient temperature of 28°C for curing, and finally a buffer-type organic soil for subsequent testing is obtained.

[0053] S3, dynamic regulation:

[0054] The buffer-type organic soil prepared in this example will be managed using the same dynamic regulation method as in Example 1 in the subsequent pot test of Chinese rose.

[0055] Comparative Example 1:

[0056] Compared with Example 1, the difference is that the preparation of the organic soil does not perform step S2 of in-situ buffer system construction, but directly mixes 750 parts of rotten leaf soil, 50 parts of perlite and 4 parts of calcium carbonate powder uniformly, and does not perform step S3 of dynamic regulation in the subsequent pot test, but only performs regular watering. The rest of the preparation conditions are the same as step S1 of Example 1.

[0057] Comparative Example 2:

[0058] The only difference compared with Example 1 is that in the pretreatment of step S1, 50 parts by weight of biochar as a cation exchange enhancer is not added. The rest of the component amounts and preparation steps are the same as Example 1.

[0059] Comparative Example 3:

[0060] Compared with Example 1, the preparation method of the buffer type organic soil is exactly the same, but the difference is that in the subsequent pot test of Chinese rose, step S3 is not performed, but only regular watering with clean water is adopted.

[0061] Test Example 1:

[0062] Experimental steps:

[0063] Respectively, 100g of the organic soil samples prepared in Examples 1-3 and Comparative Examples 1-3 and subjected to air drying treatment were respectively placed in 6 beakers of the same specification. 500mL of deionized water was added to each beaker to prepare a soil-water suspension with a mass-volume ratio of 1:5. The beakers were placed on a magnetic stirrer, and the suspension was continuously stirred at a constant speed.

[0064] The pH meter electrode calibrated with the standard buffer solution was immersed in the suspension, and after the pH meter reading was stable for more than 1 minute, the value was recorded as the initial pH value of each group of samples. Subsequently, 1.0mL of sulfuric acid solution with a concentration of 0.1mol / L was accurately added to each beaker using a 10mL pipette.

[0065] After the addition was completed, the suspension was continuously stirred, and after the pH meter reading was stable again for more than 1 minute, the pH value at this time was recorded. The addition and recording operation was repeated until the cumulative volume of sulfuric acid solution added in each group of samples reached 5.0mL.

[0066] The pH value change of each group of organic soil samples during the acid titration process is recorded in Table 1.

[0067] Table 1: pH value change of each group of organic soil samples during acid titration

[0068] Cumulative acid solution addition amount (mL) Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 0.0 7.18 7.23 7.29 7.91 7.16 7.18 1.0 7.11 7.15 7.21 6.98 6.78 7.10 2.0 7.02 7.08 7.12 6.09 6.15 7.03 3.0 6.95 7.01 7.05 5.31 5.51 6.96 4.0 6.86 6.93 6.97 4.82 4.90 6.87 5.0 6.79 6.85 6.91 4.45 4.58 6.80

[0069] From Table 1, it can be seen that:

[0070] Under the condition of continuous addition of acid solution, the pH value of the organic soil samples of Example 1, Example 2 and Example 3 decreased significantly less than that of Comparative Example 1 and Comparative Example 2. The results show that the organic soil prepared by in-situ reaction of solid-phase alkali reserve with buffer system generator in the base organic soil has obvious ability to resist the impact of external acidic substances. The source of this ability is the multi-phase buffer system formed by the liquid-phase buffer pair and the solid-phase alkali reserve in the organic soil.

[0071] When external acidic substances enter the system, they are preferentially neutralized by the weak acid ions in the liquid phase. When the liquid phase components are consumed, the calcium carbonate reserves in the solid phase slowly dissolve to replenish them, thereby maintaining the pH value fluctuation within a small range.

[0072] Comparing the results of Example 1 and Comparative Example 1, both have the same calcium carbonate content in the initial components, but the pH value of Comparative Example 1 decreases sharply under the impact of the acidic solution. This shows that simply physically mixing alkaline substances (calcium carbonate) with organic soil has limited buffering capacity, and its effect mainly depends on the slow dissolution of the substance in water. However, Example 1 pre-generates soluble liquid phase buffer pairs through in-situ reaction, building a more efficient buffering platform. At the same time, comparing Example 1 and Comparative Example 2, the pH value of Comparative Example 2 decreases significantly faster than Example 1 in the absence of cation exchange enhancer, which shows that the presence of cation exchange enhancer has a positive effect on maintaining the stability of the buffer system.

[0073] In summary, the test results confirm the core idea of the technical solution, i.e. constructing a multi-phase buffer system through in-situ chemical reaction in organic soil is the key to obtaining long-term and stable pH buffering performance.

[0074] Test Example 2:

[0075] To verify the anchoring ability of the cation exchange enhancer added in the technical solution to the key ions in the buffer system, i.e. the performance of resisting leaching loss, the following test is performed.

[0076] Experimental steps: 200g of organic soil samples prepared by Example 1 (containing cation exchange enhancer) and Comparative Example 2 (not containing cation exchange enhancer) respectively, and subjected to air drying treatment. Fill the two samples into two glass leaching columns with the same inner diameter and height, and ensure that the sample height and compactness after filling are consistent between the two columns.

[0077] Use a peristaltic pump to uniformly drip deionized water from the top of each leaching column at a constant flow rate of 5mL / min, simulating the irrigation leaching process. Collect the leachate from the bottom of the leaching column when it starts to flow out, with each 50mL of leachate as a collection batch. For each batch of leachate sample, use an atomic absorption spectrometer to measure the concentration of calcium ions (Ca 2+ ) therein and record it.

[0078] Experimental data: The measurement results of the calcium ion concentration in the leachate of the two groups of organic soil samples under different leaching volumes are recorded in Table 2.

[0079] Table 2: Calcium ion concentration in the leachate of the two groups of organic soil samples

[0080] Cumulative elution volume (mL) Example 1 Leachate Ca 2+ Concentration (mg / L) Comparative Example 2 Eluate Ca 2+ Concentration (mg / L) 50 15.7 46.2 100 14.8 41.5 150 14.2 35.8 200 12.9 29.1 250 11.1 21.9

[0081] The experimental data in Table 2 shows that, under the condition of continuous deionized water leaching, the calcium ion concentration in the leachate of the Comparative Example 2 (without the addition of cation exchange enhancer) sample is significantly higher than the corresponding value of the Example 1 sample at all measurement points. This result directly indicates that, in the system lacking the cation exchange enhancer, calcium ions are more rapidly and massively lost with the penetration of water. In contrast, the system of Example 1 exhibits effective holding capacity for calcium ions, with a slow loss rate and a low total amount.

[0082] The internal mechanism of this phenomenon lies in the fact that Example 1 introduces an enhancer (biochar) with high cation exchange capacity in the pretreatment step. This material has a large number of negative charge sites distributed on its surface and internal pore structure. After the subsequent in-situ reaction generates positively charged calcium ions (Ca 2+ ), these ions are adsorbed and fixed on the surface of the cation exchange enhancer by electrostatic attraction, forming a relatively stable anchoring structure. This structure restricts the free migration of calcium ions in the soil aqueous solution, so that most of the calcium ions are trapped inside the organic soil, and only a small amount is lost with water.

[0083] Therefore, the results of this test confirm the key role of the cation exchange enhancer in the present technical solution. By effectively anchoring the key cations generated by the in-situ reaction, the durability and stability of the entire buffer system under irrigation conditions are improved. This characteristic is the basis for ensuring that the buffer-type organic soil can maintain its pH regulation function for a long time, providing a sustained and stable pH condition for the root environment of the Chinese rose, thereby supporting the effective implementation of subsequent dynamic regulation steps.

[0084] Test Example 3:

[0085] To verify the comprehensive application effect of the present technical solution in the actual cultivation of Chinese rose, including the influence on plant growth and the absorption of key nutrient elements, the following test is performed.

[0086] Experimental steps: Select healthy cuttings of "Fenghua" Chinese rose with consistent growth and size, and randomly divide them into 6 groups, namely Example 1-3 groups and Comparative Example 1-3 groups, with 10 pots set as repeats for each group. Fill the organic soil prepared in the aforementioned examples and comparative examples into flowerpots of the same size, and transplant the Chinese rose seedlings into the pots uniformly. All the pots are placed in the same greenhouse environment to ensure the consistency of external environmental conditions such as light, temperature, and humidity.

[0087] During the 90-day experimental period, the groups were managed differently: for the Example 1-3 groups and the Comparative Example 2 group, a dynamic regulation method was used. During the first 45 days after transplanting (defined as the vegetative growth period), a citric acid-potassium dihydrogen phosphate complex solution was applied as an acidic functional regulator; during the 46th to 90th day (defined as the bud and flowering period), a potassium humate solution was applied as an alkaline functional regulator. During the management period, the pH value of the organic soil was regularly monitored using a pH meter, and according to the deviation of the measured value from the target range, the amount or frequency of the next functional regulation agent was adjusted.

[0088] For the Comparative Example 1 group and the Comparative Example 3 group, no functional regulation agent was applied during the entire experimental period, and only regular watering was performed according to the water needs of the plants. When the experiment was conducted to the 90th day, the growth indicators and nutrient content of the roses in each group were measured. The growth indicators included measuring the plant height, stem base diameter, total fresh weight of the aboveground part, and the number of flowers (including buds) per plant. The nutrient content indicators were measured by collecting mature leaves from the same part of the plants in each group, drying, and crushing them, and then using the Kjeldahl nitrogen determination method, the molybdenum-antimony anti-colorimetric method, and the flame photometric method to determine the total nitrogen, total phosphorus, and total potassium content in the leaves, respectively.

[0089] Experimental data: the growth indicator data statistics and the main nutrient element content of the leaves of the roses in each group are recorded in Tables 3 and 4, respectively.

[0090] Table 3: Growth indicator data statistics of roses in each group

[0091] Test group Average plant height (cm) Average stem diameter (mm) Average total fresh weight (g) Average number of flowers (pieces) Example 1 35.8 5.9 156.1 6.4 Example 2 36.9 6.2 163.5 6.9 Example 3 38.4 6.5 171.2 7.3 Comparative Example 1 25.1 4.2 99.8 2.6 Comparative Example 2 30.2 5.1 126.3 4.3 Comparative Example 3 28.9 4.8 120.4 3.9

[0092] Table 4: Main nutrient element content of leaves of roses in each group

[0093] Test group Total nitrogen (N) content (%) Total phosphorus (P) content (%) Total potassium (K) content (%) Example 1 2.98 0.42 2.58 Example 2 3.11 0.44 2.71 Example 3 3.19 0.46 2.80 Comparative Example 1 2.15 0.26 1.88 Comparative Example 2 2.48 0.33 2.19 Comparative Example 3 2.41 0.30 2.11

[0094] The experimental data in Tables 3 and 4 show that the growth indicators (plant height, stem diameter, fresh weight, and number of flowers) of the roses in Examples 1, 2, and 3, as well as the nitrogen, phosphorus, and potassium element content in the leaves, are all higher than those of all the comparative example groups. This result shows that the complete method provided by the present technical solution can significantly promote the growth and development of roses and the absorption and utilization of key nutrient elements. The internal mechanism lies in the fact that the method first constructs a stable rhizosphere pH environment, and on this basis, accurately regulates the plant physiological needs.

[0095] The effect is first attributed to the multi-phase buffer system built inside the organic soil. The system provides a stable pH environment for the root system, avoiding the drastic changes in the rhizosphere pH caused by conventional water and fertilizer management, and providing a basic guarantee for the healthy growth of the plant. Further, the dynamic regulation step actively adjusts the stable pH platform to the optimal range of the corresponding stage by applying specific functional regulators at different growth stages. For example, during the vegetative growth stage, the pH is adjusted to 6.0-6.5, which improves the bioavailability of nitrogen, phosphorus and various trace elements; during the budding and flowering stage, the pH is adjusted to 6.5-6.8, which is more suitable for the physiological activities of this stage. At the same time, the regulator itself (citric acid-potassium dihydrogen phosphate complex solution and potassium humate solution) adjusts the pH while directly supplementing the plant with phosphorus and potassium elements, which are in great demand at specific stages, achieving the synergistic effect of pH regulation and precise fertilization.

[0096] Comparing the results of Comparative Example 1 and Comparative Example 3, both have the same initial buffer-type organic soil, but Comparative Example 3 lags behind in all indicators due to the lack of dynamic regulation, which confirms the necessity of actively regulating pH and nutrient supply according to the growth stage. Comparing Comparative Example 1 and Comparative Example 2, both perform dynamic regulation, but Comparative Example 2 performs poorly due to the lack of cation exchange enhancer, which is consistent with the results of Test Example 2, indicating that the durability of the buffer system is crucial for maintaining good growth over a long period (90 days). In summary, the significant effect of the present technical solution is the synergistic effect of its internal technical features: a stable and durable buffer platform combined with a precise and coupled dynamic regulation process, which together form a complete technical closed loop for optimizing the root environment of Chinese rose.

Claims

1. A method of dynamically regulating organic soil of Rosa hybrida by in situ buffering, characterized by, The method comprises the following steps: S1, pretreatment: mixing and sterilizing the base organic soil containing organic carriers and physical modifiers; S2, in-situ buffer system construction: adding solid-phase alkali reservoir and buffer system generator to the base organic soil to generate in-situ reaction in the base organic soil, generating an in-situ multi-phase buffer system composed of liquid-phase buffer pairs and solid-phase alkali reservoir, and obtaining buffer-type organic soil; S3, dynamic regulation: applying functionalized regulating agents to the buffer-type organic soil at different growth stages of the Chinese rose to adjust and maintain the pH value of the buffer-type organic soil within the corresponding preset target range of each growth stage.

2. The method for dynamic regulation of organic soil of Chinese rose by in-situ buffering according to claim 1, characterized in that, In step S1, the base organic soil further comprises a cation exchange enhancer, and the pretreatment step comprises: According to the weight part, 750-850 parts of the organic carrier, 50-100 parts of the physical modifier, and 50-100 parts of the cation exchange enhancer are weighed; Mixing the weighed components for 12-18 minutes to obtain uniformly mixed base organic soil; The base organic soil is subjected to high-temperature steam sterilization at a sterilization temperature of 105-121℃ for 45-60 minutes.

3. The method for dynamic regulation of organic soil of Chinese rose by in-situ buffering according to claim 2, characterized in that, The organic carrier is leaf soil, the physical modifier is perlite, and the cation exchange enhancer is biochar or vermiculite; The cation exchange enhancer is used to anchor the cations generated by the in-situ reaction through its high cation exchange capacity.

4. The method for dynamic regulation of organic soil of Chinese rose by in-situ buffering according to claim 1, characterized in that, In step S2, the solid-phase alkali reservoir is calcium carbonate powder, and the buffer system generator is glacial acetic acid; the step of constructing the in-situ buffer system comprises: Adding 4-8 parts by weight of the calcium carbonate powder to the base organic soil after the pretreatment and mixing uniformly; 0.6-1.2 parts by weight of the glacial acetic acid is prepared into acetic acid working solution, and the acetic acid working solution is uniformly sprayed into the base organic soil containing the calcium carbonate powder to trigger the in-situ reaction.

5. The method for dynamic regulation of organic soil of Chinese rose by in-situ buffering according to claim 4, characterized in that, The preparation step of the acetic acid working solution comprises: Weigh 30-115 parts by weight of deionized water and place it in a container with a stirring device; Start the stirring device at a stirring rate of 100-300 rpm, and slowly add the 0.6-1.2 parts by weight of glacial acetic acid to the deionized water under this stirring condition; After all the glacial acetic acid is added, continue stirring for 5-10 minutes until the system is uniformly mixed to form the acetic acid working solution with a volume concentration of 1.0% to 2.0%.

6. The method for dynamic regulation of organic soil of Chinese rose by in-situ buffering according to claim 5, characterized in that, After the in-situ reaction, the organic soil is further subjected to a maturation process, the specific process being: The organic soil after the in-situ reaction is stacked and sealed with a film; The sealed organic soil is left to stand at an ambient temperature of 15-28℃ for 50-75 hours to allow the in-situ multi-phase buffer system to fully form and stabilize, thereby obtaining the buffer-type organic soil.

7. The method for dynamic regulation of organic soil of Chinese rose by in-situ buffering according to claim 1, characterized in that, In step S3, the dynamic regulation step comprises: Determine the growth stage of the Chinese rose to determine the corresponding preset target range; When it is determined that the rose is in the vegetative growth stage, an acidic functional regulator is selected as the functional regulator to be applied to adjust and maintain the pH value of the buffer-type organic soil within the preset target range of 6.0-6.5; When it is determined that the rose is in the bud-bearing and flowering stage, an alkaline functional regulator is selected as the functional regulator to be applied to adjust and maintain the pH value of the buffer-type organic soil within the preset target range of 6.5-6.

8.

8. The method for dynamic regulation of organic soil of Chinese rose by in-situ buffering according to claim 7, characterized in that, In step S3, the dynamic regulation process comprises: The acidic functional regulator is a citric acid-potassium dihydrogen phosphate compound solution, which functions to lower the pH value of the buffer-type organic soil, supplement phosphorus and potassium elements for the rose, and chelate and activate the trace elements in the buffer-type organic soil; The alkaline functional regulator is a potassium humate solution, which functions to raise the pH value of the buffer-type organic soil, supplement potassium elements for the rose, and stimulate root growth.

9. The method for dynamic regulation of organic soil of Chinese rose by in-situ buffering according to claim 8, characterized in that, The method further comprises a monitoring feedback step running during the execution of S3, which comprises the following steps: Periodically measure the pH value of the buffer-type organic soil using a pH meter to obtain a real-time pH measurement value; Compare the real-time pH measurement value with the preset target range of the current growth stage to obtain a deviation value; When the deviation value is greater than 0.2, adjust the dosage or application frequency of the functional regulator to be applied next time according to the deviation value.

10. The method for dynamic regulation of organic soil of Chinese rose by in-situ buffering according to claim 6, characterized in that, The buffer-type organic soil is prepared by in-situ reaction and aging of a base organic soil containing an organic carrier, a physical modifier, and a cation exchange enhancer, with calcium carbonate powder as a solid-phase alkali reservoir and glacial acetic acid as a buffer system generator.

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