Preparation method for improving skin-friendly performance and stability of make-up fixing spray

Abstract

The invention belongs to the technical field of make-up products, and particularly relates to a preparation method for improving skin friendliness and stability of make-up spray. The makeup fixing product coated with a film is prepared by compounding active ingredients and adopting O / W / O multiple emulsification and microencapsulation technologies, anti-inflammatory, anti-ultraviolet, anti-blue-light and skin barrier repair are realized on skin friendliness, meanwhile, protein impurities are reduced in the fermentation process, sensitive skin is adapted, and the retention rate of skin moisture in 24 hours exceeds 99%; in the aspect of durability, the product is resistant to migration, sweat and water, and in the aspect of stability, an O / W / O system is subjected to optimized process treatment, so that multiple stabilities of high-temperature storage, low-temperature circulation without crystallization and centrifugation without layering are realized; on the aspects of moisturizing, oil control and balance, 24-hour moisture retention is guaranteed, and excessive sebaceous gland secretion is inhibited. Multiple breakthroughs of moisturizing, oil control, anti-inflammation, protection and stability are achieved, and practical application value is achieved.

Description

[0001] The present application claims priority to the following patent application: Chinese patent application No. 2024111929212, filed on August 28, 2024, and entitled "Preparation method for improving skin friendliness and stability of setting spray". TECHNICAL FIELD

[0002] The present application belongs to the technical field of setting products, and specifically relates to a preparation method for improving skin friendliness and stability of setting spray. BACKGROUND

[0003] At present, with the pursuit of beautiful makeup by the public, the requirements for the durability and stability of setting products on makeup have been improved to prevent makeup from being removed or damaged due to sweat and oil secretion caused by activities such as life and work. Setting spray is a kind of setting product, and it has become a trend product because of its convenience and ability to quickly form a protective film on the facial skin. Water is the main component in the formula of setting spray, and film-forming agents, moisturizing agents, and oil-controlling ingredients are also added. The main emphasis is on the moisturizing effect, which slows down the removal of makeup by reducing oil secretion. In addition, setting spray containing film-forming agents can quickly form a protective film on the skin surface after makeup, reducing water evaporation on the skin surface, and achieving the effects of moisturizing and balancing oil.

[0004] With the development of technology, the formula and technology of setting spray are constantly innovating, from the initial simple water-based spray to the current professional setting spray containing various functional ingredients. Its functions have also developed from simply fixing makeup to combining moisturizing, sunscreen, and oil control. The common setting sprays on the market have the following shortcomings: limited makeup holding time: some setting sprays have poor makeup holding effect and are prone to makeup removal, especially in hot and humid weather or long outdoor activities; large spray particles: some setting sprays have large spray particles, which can cause uneven makeup and even block pores; single ingredients: many setting sprays have overly simple formulas, lack of functional ingredients for different skin types and makeup needs, and are difficult to meet individual needs; safety issues: some setting sprays may contain irritating ingredients, which can cause skin irritation or other adverse reactions.

[0005] Therefore, in order to promote the development of setting spray technology and provide consumers with safer, more effective, and convenient makeup products, the present application proposes to improve the comfort and naturalness of the skin while achieving the moisturizing, oil control, and durability of setting products. SUMMARY

[0006] In view of the above problems, the purpose of the present application is to provide a preparation method for improving the skin friendliness and stability of setting spray.

[0007] The technical content of the present application is as follows:

[0008] The application provides a preparation method for improving the skin friendliness and stability of a makeup spray, comprising the following steps:

[0009] 1) oil-in-water O / W treatment: hydrophilic emulsifiers are added to a water phase mixture, heated, dissolved, and stirred uniformly to obtain an emulsion, an inner oil phase is heated, dissolved, and slowly added to the emulsion, then PEG-40 hydrogenated castor oil is added, and stirring is performed to uniformly disperse, and then ultrasonic treatment is performed, to obtain an O / W emulsion;

[0010] The hydrophilic emulsifiers include glycerol polyether-26, cetyl stearyl polyether-12, cetyl stearyl polyether-20, and sucrose stearate, and the mass ratio is (3-4):(1-1.6):(3-4):(1-1.2).

[0011] The water phase mixture is prepared by mixing and stirring water, active ingredients, glycerol, ethylhexylglycerin, p-hydroxyacetophenone, nicotinamide, and disodium EDTA uniformly, at a stirring temperature of 50-60 DEG C and a stirring speed of 200-300 r / min, to obtain the water phase mixture.

[0012] The active ingredients are obtained by previously dissolving HAMAMELIS VIRGINIANA water, GENTIANA SCABRA extract, STEPHANIA TETRANDRA extract, and 3-o-ethyl ascorbic acid in an organic solution of ethanol and butanediol.

[0013] According to the mass fraction, the active ingredients account for 10-15%, the glycerol accounts for 25-35%, the ethylhexylglycerin accounts for 5-10%, the p-hydroxyacetophenone accounts for 0.5-1%, the nicotinamide accounts for 2-5%, the disodium EDTA accounts for 2-5%, and water accounts for the rest to 100%.

[0014] According to the mass fraction, in the active ingredients, the HAMAMELIS VIRGINIANA water accounts for 5-15%, the GENTIANA SCABRA extract accounts for 1-1.5%, the STEPHANIA TETRANDRA extract accounts for 10-20%, the 3-o-ethyl ascorbic acid accounts for 10-20%, the ethanol accounts for 15-25%, and the butanediol accounts for 20-35%.

[0015] The HAMAMELIS VIRGINIANA water contains HAMAMELIS VIRGINIANA extract, is rich in tannic acid, has the effects of antioxidation and anti-inflammation, and can soothe and improve sensitive skin.

[0016] The gentian (GENTIANA SCABRA) extract is mainly the extract of gentian root, has the effects of anti-inflammatory, bacteriostasis and oxidation resistance, can stimulate the generation of skin collagen, and helps to repair damaged skin.

[0017] The stephania tetrandra (STEPHANIA TETRANDRA) extract contains the main component of tetrandrine, has the effects of anti-inflammatory, anti-allergic and oxidation resistance, can also repair damaged skin tissue, and effectively relieves the skin.

[0018] The polygonum orientale polysaccharide / fermentation broth is obtained by inoculating activated bacillus siamensis into a polygonum orientale polysaccharide aqueous solution, then performing fermentation at 35℃, and then evaporating water to obtain the polysaccharide. The polygonum orientale polysaccharide is obtained by fermentation, can adsorb water molecules, forms a stable structure in the emulsified product, forms a protective film on the skin, and maintains the hydration state of the skin. The polygonum orientale polysaccharide has the effects of soothing the skin, repairing the skin barrier, moisturizing, oxidation resistance, and improving skin affinity.

[0019] The inoculation concentration of the bacillus siamensis is 1.5×10 7 ~2×10 7 CFU / mL of the polysaccharide aqueous solution, and the concentration of the polygonum orientale polysaccharide aqueous solution is 15~20%.

[0020] The preparation of the polygonum orientale polysaccharide includes:

[0021] a) extraction: the polygonum orientale material is extracted by hot water, precipitated by ethanol, and purified by centrifugation to obtain polygonum orientale crude polysaccharide;

[0022] b) purification: the polygonum orientale crude polysaccharide is extracted by hot water, then precipitated by ethanol, centrifuged, and then subjected to DEAE-52 cellulose column chromatography and freeze-drying to obtain polygonum orientale polysaccharide with a purity of ≥90%.

[0023] The addition amount of the hydrophilic emulsifier accounts for 2~6wt% of the water phase mixture;

[0024] The addition amount of the internal oil phase accounts for 3~6wt% of the emulsified product;

[0025] The internal oil phase includes one or more of tocopheryl acetate and cetyl stearyl alcohol isononanoate;

[0026] The addition amount of the PEG-40 hydrogenated castor oil accounts for 2~3wt% of the emulsified product;

[0027] The temperature of the heating and dissolving is in the range of 60~70℃, and the dissolving is completed when the temperature reaches the range;

[0028] The homogenization and dispersion is performed at a rotation speed of 2000~3500r / min for 2~5 minutes.

[0029] The ultrasonic power of the ultrasonic treatment is 300-400 W, and the time is 10-20 min;

[0030] 2) O / W / O microencapsulation treatment: add lipophilic emulsifier into the outer oil phase, heat and dissolve, slowly and uniformly, then add microencapsulation agent, the mass ratio is (1-1.5):(3-4):(1.5-2.5), homogenously disperse at a speed of 800-1000 r / min for 8-10 min, then perform ultrasonic treatment at a power of 100-200 W for 5-10 min, reduce to normal temperature, then slowly add O / W emulsion into the outer oil phase at a volume ratio of (4-5):(5-6) by using a syringe pump, homogenously disperse at a speed of 3000-5000 r / min for 3-6 min, obtain O / W / O microencapsulation product, which is the makeup fixing product, and the makeup fixing product is used by a sprayer to obtain makeup fixing spray;

[0031] The temperature of the heat and dissolution is 70-80℃, and dissolution is achieved;

[0032] The outer oil phase includes a mixture of tocopheryl acetate, dimethicone / vinyl dimethicone crosspolymer and silica, wherein the mass fraction of the tocopheryl acetate is 75-85%, the mass fraction of the dimethicone / vinyl dimethicone crosspolymer is 10-15%, and the mass fraction of the silica is 5-8%;

[0033] The particle size of the silica used is 2-15 μm, and the oil absorption value is 80-500 g / 100 g;

[0034] The components of the lipophilic emulsifier include cetyl stearyl alcohol isononanoate, cetyl stearyl alcohol stearate and cetyl alcohol palmitate, wherein the mass fraction of the cetyl stearyl alcohol isononanoate is 25-40%, the mass fraction of the cetyl stearyl alcohol stearate is 25-40%, and the mass fraction of the cetyl alcohol palmitate is 25-50%;

[0035] The microencapsulating agent is prepared from polyester-5, cetylstearyl alcohol and sodium hyaluronate, and the specific operation is as follows: 15-25% polyester-5 and 2-5% sodium hyaluronate are mixed, heated to 80-100 DEG C, and stirred uniformly, then 75-85% cetylstearyl alcohol is added to perform homogenization dispersion, the homogenization dispersion is performed at a rotating speed of 2000-3500 r / min for 2-5 minutes, polyester-5 and sodium hyaluronate are crosslinked in cetylstearyl alcohol through emulsification-hybridization to form a microcapsule film, and the slow release and skin targeting release of active ingredients are realized; the lipophilic emulsifier disperses the external oil phase to form a stable emulsion, provides smooth and soft touch for the skin, controls oil secretion, and makes the makeup more durable; the microencapsulating agent is processed by ultrasonic treatment to form a film structure wrapping the O / W emulsion, an O / W / O microcapsule structure is formed, polyester-5 and sodium hyaluronate are both high molecular polymers and have film forming property, after contacting the skin, they form a film on the skin surface, and the components in the emulsion are gradually released and penetrated into the skin, which is beneficial to improving the skin friendliness, stability and durability of the makeup product.

[0036] The beneficial effects of the present application are as follows:

[0037] The preparation method for improving the skin friendliness and stability of the makeup spray of the present application prepares a film-wrapped makeup product through active ingredient compounding, O / W / O multiple emulsification and microencapsulation technology, in the skin friendliness, under the synergistic effect of hamamelis virginiana water, gentiana extract, radix stephaniae tetrandrae extract, polygonum aviculare polysaccharide / fermentation broth and 3-o-ethyl ascorbic acid, anti-inflammatory, anti-ultraviolet, anti-blue light and skin barrier repair are realized, meanwhile, the fermentation process reduces protein impurities, is suitable for sensitive skin and the 24h skin moisture retention rate is more than 99%; in the durability, the crosslinked wrapping film formed by polyester-5, sodium hyaluronate and cetylstearyl alcohol makes the product anti-migration, water and sweat resistant, cooperates with the oil control effect of the fermentation broth lipopeptide substance, and effectively prolongs the makeup fresh and durable time to 14h; in the stability, the O / W / O system is treated by an optimized process, multiple stability of high-temperature storage, low-temperature circulation, no crystallization and no stratification in centrifugation is realized; in the moisture control and oil balance, sodium hyaluronate, hamamelis virginiana water and oil control ingredients synergize, both ensure 24h moisture retention and inhibit the excessive secretion of sebaceous glands, avoiding "dry" or "oily". In summary, through the combination of ingredient and process innovation, the present application solves the technical problems of short makeup duration, poor skin feeling and single function of the makeup product, realizes multiple breakthroughs in moisture retention, oil control, anti-inflammatory, protection and stability, and has practical application value. BRIEF DESCRIPTION OF DRAWINGS

[0038] Figure 1 The particle size distribution graph of the makeup spray product of the present application;

[0039] Figure 2 The anti-ultraviolet test result graph of the makeup spray product of the present application;

[0040] Figure 3 Figure for anti-blue light test results of the setting makeup spray product of the present application;

[0041] Figure 4 Figure for anti-inflammatory and moisturizing capacity test results of the setting makeup spray product of the present application. DETAILED DESCRIPTION

[0042] The present application will be further described in the following with specific examples and the accompanying drawings, it should be understood that these examples are only used to illustrate the present application and not used to limit the protection scope of the present application, after reading the present application, various equivalent modifications of the present application by those skilled in the art all fall within the claims of the present application.

[0043] Unless otherwise specified, all raw materials and reagents of the present application are conventional market raw materials and reagents.

[0044] Referring to the preparation of polysaccharide of P. fulvotecta in the article "Study on polysaccharide components and antiviral activity of P. fulvotecta", the preparation of polysaccharide of P. fulvotecta used in the present application is as follows:

[0045] P. fulvotecta medicine from Xinjiang is purchased and soaked in deionized water with a solid-liquid ratio of 1:10 kg / L, after soaking overnight, heating at 80℃ for 2-3h, extracting 2-3 times, until the extract is concentrated to a thick syrup, centrifuging to remove insoluble precipitates, adding anhydrous ethanol to the supernatant to a final ethanol concentration of 90%, standing overnight, centrifuging for 15 minutes, to obtain crude polysaccharide of P. fulvotecta;

[0046] Hot water extraction of P. fulvotecta crude polysaccharide (80℃, 2h)→ ethanol precipitation (90% ethanol)→ centrifugation (5000r / min, 15min)→ DEAE-52 cellulose column chromatography (eluent: 0.1-0.5mol / L NaCl solution)→ freeze-drying (-50℃, 24h), to obtain polysaccharide with a purity of ≥90%, and the weight average molecular weight of the polysaccharide after purification is 7.4×10 4 Da.

[0047] Preparation of polysaccharide / fermentation broth of P. fulvotecta of the present application:

[0048] Bacillus siamensis (purchased from Beijing Tianyou Fukang Biology) is purchased and activated in nutrient broth agar (peptone 5.0g, beef extract powder 3.0g, NaCl 5.0g, agar 15.0g, distilled water 1000.0mL, pH 7.0), then inoculated into the fermentation broth at a concentration of 1.5×10 7 ~2×10 7 CFU / mL (preferably 2×10 7After the 35℃ fermentation for 48h in the polysaccharide water solution of Comastelum chingii Huang, the polysaccharide is extracted by evaporating water;

[0049] The polysaccharide water solution of Comastelum chingii Huang is prepared by dissolving the extracted polysaccharide in deionized water, and the concentration is 15-20%.

[0050] The polysaccharide obtained from Comastelum chingii Huang contains polysaccharide and protein components, and the fermentation liquid after fermentation is tested, and the polysaccharide content in the 0.50g / L fermentation liquid is 46.2mg / L, the protein content is 2.4mg / L, the lipopeptide (surfactin) content is 0.67mg / L, and the total antioxidant activity of VC is 0.102g / L, which reduces the protein impurities, retains the antioxidant function of polysaccharide, and the lipopeptide can synergistically bring the effects of antibacterial, anti-inflammatory and penetration promotion.

[0051] The particle size of the silica used in the application is 2-15um, and the oil absorption value is 80-500g / 100g.

[0052] Example 1

[0053] A preparation method for improving the skin friendliness and stability of makeup spray

[0054] 1) O / W treatment: 3wt% of hydrophilic emulsifier is added to the water phase mixture, heated to 65℃, and uniformly stirred to obtain an emulsion, 4wt% of inner oil phase (mass ratio of tocopherol acetate to cetylstearyl alcohol isopropyl is 1:3) is heated to 65℃, slowly added to the emulsion, then 2-3wt% of PEG-40 hydrogenated castor oil is added, and homogenously dispersed at a speed of 3000r / min for 3 minutes, and then ultrasonic treatment is carried out at an ultrasonic power of 400W for 10min to obtain an O / W emulsion;

[0055] The hydrophilic emulsifier comprises glycerol polyether-26, cetylstearyl alcohol polyether-12, cetylstearyl alcohol polyether-20 and sucrose stearate in a mass ratio of 3.4:1.6:4:1.

[0056] The preparation of the water phase mixture is as follows: 44.2% of water, 12% of active ingredients, 30% of glycerol, 8% of ethylhexyl glycerin, 0.8% of p-hydroxyacetophenone, 3% of nicotinamide and 2% of disodium EDTA are mixed and uniformly stirred, the stirring temperature is 55℃, and the stirring speed is 250r / min to obtain the water phase mixture.

[0057] The active ingredient is 10% Hamamelis virginiana water, 1% Gentiana scabra extract, 15% Stephania tetrandra extract, 8% Cynanchum atratum polysaccharide / fermentation broth, and 15% 3-o-ethyl ascorbic acid, which are dissolved in advance in an organic solution of 20% ethanol and 31% butanediol;

[0058] The Cynanchum atratum polysaccharide / fermentation broth is obtained by inoculating 2x10 7 CFU / mL of activated Bacillus siamensis into an 18% Cynanchum atratum polysaccharide aqueous solution, and then performing 48h fermentation at 37°C, followed by evaporation to obtain;

[0059] 2) O / W / O microencapsulation treatment: add the lipophilic emulsifier into the external oil phase, heat to 75°C, slowly and uniformly, then add the microencapsulating agent with a mass ratio of 1.2:3.5:2, homogenously disperse at a speed of 900r / min for 8 minutes, then perform ultrasonic treatment at a power of 150W for 8 minutes, reduce to room temperature, then slowly add the O / W emulsion into the external oil phase with a volume ratio of 4:6 using a syringe pump, homogenously disperse at a speed of 3500r / min for 5 minutes, to obtain the O / W / O microencapsulated product, which is the makeup fixing product, and the makeup fixing product is used by a sprayer to obtain the makeup fixing spray;

[0060] The external oil phase includes a mixture of 82% tocopheryl acetate, 10% dimethicone / vinyl dimethicone crosspolymer, and 8% silica;

[0061] The components of the lipophilic emulsifier include 30% cetearyl isononanoate, 30% cetearyl stearate, and 40% cetyl palmitate;

[0062] The preparation of the microencapsulating agent is as follows: first, mix 15% polyester-5 and 5% sodium hyaluronate, heat to 90°C, and stir uniformly, then add 80% cetearyl alcohol, and homogenously disperse at a speed of 3000r / min for 4 minutes to obtain the product, wherein polyester-5 and sodium hyaluronate are dispersed in cetearyl alcohol to form crosslinking through emulsification; the lipophilic emulsifier disperses the external oil phase to form a stable emulsion, providing smooth and soft touch for the skin, controlling oil secretion, and making the makeup more durable; the microencapsulating agent forms a film structure wrapping the O / W emulsion through ultrasonic treatment, forming an O / W / O microcapsule structure, and polyester-5 and sodium hyaluronate are both high-molecular polymers and have film-forming properties, which form a film on the skin surface after contacting the skin, and the components in the emulsion are gradually released and penetrate into the skin, which is beneficial to improving the skin-friendliness, stability, and durability of the makeup fixing product.

[0063] Example 2

[0064] A preparation method for improving the skin friendliness and stability of makeup fixing spray

[0065] 1) O / W treatment: add 2wt% hydrophilic emulsifier to the water phase mixture, heat to 68℃, stir evenly to obtain emulsion, add 3wt% inner oil phase (mass ratio of tocopheryl acetate and cetylstearyl alcohol isopropyl myristate is 1:4) heated to 68℃, slowly add to the emulsion, then add 2wt% PEG-40 hydrogenated castor oil, homogenously disperse at a speed of 2500r / min for 4 minutes, then ultrasonic treatment at a power of 300W for 20 minutes, to obtain O / W emulsion;

[0066] The hydrophilic emulsifier includes glycerol polyether-26, cetylstearyl alcohol polyether-12, cetylstearyl alcohol polyether-20, and sucrose stearate in a mass ratio of 3:1:3:1;

[0067] Mix and stir evenly water 39%, active ingredient 15%, glycerol 35%, ethylhexyl glycerin 5%, p-hydroxyacetophenone 1%, nicotinamide 2%, disodium EDTA 3%, the stirring temperature is 50℃, the stirring speed is 300r / min, to obtain the water phase mixture;

[0068] The active ingredient is 15% HAMAMELIS VIRGINIANA water, 1% GENTIANA SCABRA extract, 14% STEPHANIA TETRANDRA extract, 10% Stachys Floridana polysaccharide / fermentation broth, and 15% 3-o-ethyl ascorbic acid, which are previously dissolved in an organic solution of 25% ethanol and 20% butanediol to obtain;

[0069] The Stachys Floridana polysaccharide / fermentation broth is obtained by inoculating 2×10 7 CFU / mL of activated Bacillus siamensis into a 20% Stachys Floridana polysaccharide aqueous solution, and then evaporating water to obtain after 48h fermentation at 37℃;

[0070] 2) O / W / O microencapsulation treatment: add lipophilic emulsifier to the outer oil phase, heat to 70℃, slowly and evenly, then add microencapsulating agent in a mass ratio of 1:3:1.5, homogenously disperse at a speed of 1000r / min for 8 minutes, then ultrasonic treatment at a power of 200W for 5 minutes, and then reduce to room temperature, then slowly add O / W emulsion to the outer oil phase in a volume ratio of 5:5 by using a syringe pump, homogenously disperse at a speed of 3000r / min for 6 minutes, to obtain O / W / O microencapsulated product, which is the makeup fixing product, and the makeup fixing product is used by a sprayer to obtain makeup fixing spray;

[0071] The outer oil phase includes a mixture of 80% tocopheryl acetate, 15% dimethicone / vinyl dimethicone crosspolymer and 5% silica;

[0072] The components of the lipophilic emulsifier include 40% cetearyl isononanoate, 25% cetearyl stearate and 35% cetyl palmitate;

[0073] The preparation of the microencapsulating agent is to mix 22% polyester-5 and 3% sodium hyaluronate, heat to 80℃, and stir uniformly, then add 75% cetearyl alcohol, and perform high-speed homogenization dispersion at a rotation speed of 2500r / min for 5 minutes to obtain.

[0074] Example 3

[0075] A preparation method for improving the skin friendliness and stability of makeup spray

[0076] 1) O / W treatment: add 6wt% of the hydrophilic emulsifier to the water phase mixture, heat to 63℃, and stir uniformly to obtain an emulsion, add 4wt% of the inner oil phase (tocopheryl acetate and cetearyl isononanoate in a mass ratio of 1:4) heated to 60℃, slowly add to the emulsion, then add 3wt% of PEG-40 hydrogenated castor oil, and perform homogenization dispersion at a rotation speed of 3500r / min for 2 minutes, and then perform ultrasonic treatment at an ultrasonic power of 300W for 20 minutes to obtain an O / W emulsion;

[0077] The hydrophilic emulsifier includes glycereth-26, ceteareth-12, ceteareth-20 and sucrose stearate in a mass ratio of 4:1.6:4:1.2;

[0078] The preparation of the water phase mixture is to mix and stir uniformly water 43.5%, active ingredients 13%, glycerol 25%, ethylhexylglycerin 10%, p-hydroxyacetophenone 0.5%, nicotinamide 3% and disodium EDTA 5%, with a stirring temperature of 60℃ and a stirring speed of 200r / min to obtain the water phase mixture;

[0079] The active ingredients are 5% HAMAMELIS VIRGINIANA water, 1.5% GENTIANA SCABRA extract, 20% STEPHANIA TETRANDRA extract, 5% polygonum aviculare polysaccharide / fermentation broth and 13.5% 3-o-ethyl ascorbic acid, which are previously dissolved in an organic solution of 20% ethanol and 35% butanediol to obtain;

[0080] The polygonum aviculare polysaccharide / fermentation broth is activated B. siamensis with a concentration of 1.5×107 CFU / mL inoculated into a 15% concentration of polysaccharide water solution of Cynanchum auriculatum R. Br. was fermented at 37°C for 48h, and then water was evaporated to obtain;

[0081] 2) O / W / O microencapsulation treatment: add lipophilic emulsifier to the outer oil phase, heat to 80°C, slowly and uniformly, then add microencapsulating agent with a mass ratio of 1.5:4:2.5, homogenously disperse at a speed of 800 r / min for 10 minutes, then perform ultrasonic treatment at a power of 100W for 10 minutes, reduce to room temperature, then slowly add O / W emulsion to the outer oil phase with a volume ratio of 4:6 using a syringe pump, homogenously disperse at a speed of 4000 r / min for 5 minutes, obtain O / W / O microencapsulated product, which is the makeup fixing product, and use the makeup fixing product through a sprayer to obtain a makeup fixing spray;

[0082] The outer oil phase includes a mixture of 77% tocopheryl acetate, 15% dimethicone / vinyl dimethicone crosspolymer, and 8% silica;

[0083] The components of the lipophilic emulsifier include 40% cetearyl isononanoate, 25% cetearyl stearate, and 35% cetyl palmitate;

[0084] The preparation of the microencapsulating agent is as follows: first, mix 23% polyester-5 and 2% sodium hyaluronate, heat to 100°C, and stir uniformly, then add 85% cetearyl alcohol, and homogenously disperse at a speed of 3500 r / min for 3 minutes to obtain.

[0085] Example 4

[0086] A preparation method for improving the skin friendliness and stability of a makeup fixing spray

[0087] 1) O / W treatment: add 5wt% hydrophilic emulsifier to the water phase mixture, heat to 70°C, and stir uniformly to obtain an emulsion, then slowly add 6wt% inner oil phase (tocopheryl acetate and cetearyl isononanoate with a mass ratio of 2:5) to the emulsion, then add 3wt% PEG-40 hydrogenated castor oil, homogenously disperse at a speed of 3000 r / min for 4 minutes, then perform ultrasonic treatment at a power of 300W for 15 minutes to obtain O / W emulsion;

[0088] The hydrophilic emulsifier includes glycereth-26, ceteareth-12, ceteareth-20, and sucrose stearate with a mass ratio of 3:1.5:3.5:1;

[0089] The preparation of the water phase mixture is: mixing and stirring uniformly water 43.2%, active ingredient 10%, glycerol 30%, ethylhexylglycerin 8%, p-hydroxyacetophenone 0.8%, nicotinamide 5%, disodium EDTA 3% at a stirring temperature of 55℃ and a stirring speed of 250r / min to obtain the water phase mixture;

[0090] The active ingredient is 15% HAMAMELIS VIRGINIANA water, 1.5% GENTIANA SCABRA extract, 13.5% STEPHANIA TETRANDRA extract, 10% MACLEAYA CORDATA polysaccharide / fermentation broth and 10% 3-o-ethyl ascorbic acid, which are previously dissolved in an organic solution of 15% ethanol and 35% butanediol to obtain;

[0091] The MACLEAYA CORDATA polysaccharide / fermentation broth is obtained by inoculating 1.5×10 7 CFU / mL of activated Bacillus siamensis into a 20% MACLEAYA CORDATA polysaccharide aqueous solution and then performing 48h fermentation at 37℃ to obtain by evaporation of water;

[0092] 2) O / W / O microencapsulation treatment: the lipophilic emulsifier is added to the outer oil phase, heated to 75℃, slowly and uniformly, then the microencapsulating agent is added at a mass ratio of 1:3:2, and homogenously dispersed at a speed of 800r / min for 10 minutes, then ultrasonic treatment is performed at a power of 100W for 10 minutes, then the temperature is lowered to room temperature, then the O / W emulsion is slowly added to the outer oil phase at a volume ratio of 5:5 using a syringe pump, and homogenously dispersed at a speed of 3500r / min for 5 minutes to obtain the O / W / O microencapsulated product, which is the makeup fixing product, and the makeup fixing product is used by a sprayer to obtain the makeup fixing spray;

[0093] The outer oil phase includes a mixture of 80% tocopheryl acetate, 13% dimethicone / vinyl dimethicone crosspolymer and 7% silica;

[0094] The components of the lipophilic emulsifier include 35% cetearyl isononanoate, 40% cetearyl stearate and 25% cetyl palmitate;

[0095] The preparation of the microencapsulating agent is: first, 20% polyester-5 and 5% sodium hyaluronate are mixed, heated to 85℃, stirred uniformly, then 75% cetearyl alcohol is added, and high-speed homogenously dispersed at a speed of 3000r / min for 4 minutes to obtain.

[0096] Comparative Example 1

[0097] As a control group of Example 1, in the preparation of the makeup fixing spray of Comparative Example 1, the extract of Stephania tetrandra is not used in the active ingredient, and the others remain unchanged.

[0098] Comparative Example 2

[0099] As a control group of Example 1, in the preparation of the makeup fixing spray of Comparative Example 1, the extract of Stephania tetrandra is not used in the active ingredient, and the others remain unchanged.

[0100] Comparative Example 3

[0101] As a control group of Example 1, in the preparation of the makeup fixing spray of Comparative Example 1, the extract of Stephania tetrandra is not used in the active ingredient, and the others remain unchanged.

[0102] Comparative Example 4

[0103] As a control group of Example 1, in the preparation of the makeup fixing spray of Comparative Example 1, the extract of Stephania tetrandra is not used in the active ingredient, and the others remain unchanged.

[0104] Comparative Example 5

[0105] As a control group of Example 1, in the preparation of the makeup fixing spray of Comparative Example 1, the extract of Stephania tetrandra is not used in the active ingredient, and the others remain unchanged.

[0106] 1. Particle size distribution test

[0107] From Figure 1 It can be seen that the particle size distribution test results of the makeup fixing spray products prepared in Examples 1-4 are shown in the figure, and according to the number distribution, the average particle size is 67.282 μm, and the dispersion is relatively uniform.

[0108] 2. Human skin patch test

[0109] 32 subjects were selected, 9 men and 32 women, aged 20-40 years, meeting the subject volunteer selection criteria;

[0110] Select qualified patch test materials, with closed patch test method, about 0.020-0.025 mL of the make-up spray product of the application is placed in the patch test device, and the low sensitization adhesive tape is applied to the flexor of the forearm of the subject, the test product is removed after 24 hours, and the skin reaction is observed at 0.5, 24 and 48 hours after removal, and the results show that 0 people have skin adverse reactions.

[0111] 3. Antioxidant test

[0112] Reagent: positive control: ascorbic acid; DHHP (1,1-diphenyl-2-trinitrobenzene hydrazine); 95% ethanol;

[0113] Dilute the positive control with water to a series of concentration gradients of 0.020 mg / mL, 0.015 mg / mL, 0.010 mg / mL, 0.005 mg / mL, and 0.002 mg / mL for verification of the test system;

[0114] Dilute the make-up spray product with water to a concentration of 20%, and use the 0% sample solution as the negative control;

[0115] Use the DPPH scavenging activity evaluation method as the determination method of antioxidant activity, use a 96-well microplate to set up sample wells (T), sample background (T0), DPPH wells (C), and Rongji background (C0), and for each concentration of each sample, 3 wells of sample wells (T) are set up in parallel, and 3 wells of DPPH wells (C) are also set up in parallel.

[0116] Supplement the solvent in the used micro-wells, the sample is water, and 0.1 mL is supplemented, and shaken well;

[0117] Add DPPH ethanol solution 0.1 mL in the sample wells (T) and DPPH wells (C), and use 95% ethanol instead of the sample background (T0) and the solvent background (C0), and shake gently, and stand at room temperature for 5 minutes;

[0118] If there are bubbles in the micro-well containers, ultrasonic them for 1-2 seconds to eliminate, wipe the bottom of the micro-well plate dry, put it into an enzyme marker, and measure at 517 nm;

[0119] Elimination rate X (%) = [1-(T-T0) / (C-C0)]x100%;

[0120] Wherein, T is the absorbance of the sample well, T0 is the absorbance of the sample background, C is the absorbance of the DPPH well (average value), and C0 is the absorbance of the solvent background;

[0121] The test results take the average of three data, and the results are shown in the following table:

[0122] Table 1 Antioxidant test results

[0123] Radical elimination rate (%) Example 1 93.7 Example 2 93.5 Example 3 93.9 Example 4 92.1 Negative control group 0.82 Comparative Example 1 87.6 Comparative Example 2 83.3 Comparative Example 3 57.5 Comparative Example 4 90.8 Comparative Example 5 89.8

[0124] As shown in Table 1, the prepared makeup spray has certain antioxidant properties, while the comparative examples show that the absence of the powder aconite extract, the radix comphoric polysaccharide / fermentation liquid and the active ingredients containing the components have a more significant influence on the antioxidant properties, and the change in the preparation process slightly affects the antioxidant properties due to the influence on the release of the active ingredients.

[0125] 4. Oil control test;

[0126] The enzyme inhibition rate method QTTZ-XZ-39 "Cosmetic oil control efficacy verification and detection details" is used for testing. 5α-reductase (5α-R) is an important androgen metabolic enzyme in the skin, which can irreversibly convert testosterone (T) into dihydrotestosterone (DHT). DHT is the most active androgen, which can induce excessive secretion of sebum by sebaceous glands. By inhibiting the activity of 5α-reductase to reduce the level of DHT, the excessive secretion of sebum by sebaceous glands can be effectively alleviated. The 5α-reductase inhibition rate of the test sample is tested by the above principle to determine whether the sample has an oil control effect. The calculation formula is as follows:

[0127] Inhibition rate (%) = (1-T / C) x 100%;

[0128] In the formula, T is the 5α-reductase content of the test sample solution; C is the 5α-reductase content of the negative control.

[0129] The results are shown in the following table:

[0130] Table 2 Oil control test results

[0131] 5α-reductase content (pg / mL) Inhibition rate / % Example 1 26.77 64.10％ Example 2 25.84 65.35％ Example 3 26.21 64.85％ Example 4 25.92 65.24％ Negative control group 74.57 - Comparative Example 1 29.25 60.78％ Comparative Example 2 30.84 58.64％ Comparative Example 3 59.48 20.24％ Comparative Example 4 46.97 37.01％ Comparative Example 5 37.04 50.33％

[0132] As shown in Table 2, the makeup fixing spray product of the embodiment of the present application has an inhibition rate of 5a-reductase of greater than 64%, and the enzyme content is significantly lower than that of the negative control group, proving that the active ingredient can efficiently inhibit the key enzyme of oil synthesis; after analysis by SPSS software, a double-tailed test is adopted, and the significant factor P < 0.05, indicating that the difference is significant, indicating that the prepared makeup fixing spray product has oil control effect, which can also be seen from the effects of Comparative Examples 1-3, and the absence of active ingredients leads to oil control failure, and the inhibition rate of Comparative Example 3 is only 20.24%, and the 5a-reductase content is close to that of the negative control group, indicating that the radix Stephaniae tetrandrae extract (containing tetrandrine) and the radix Anemone vitifolii polysaccharide / fermentation broth (containing lipopeptide) are the core of oil control; as shown in Comparative Example 4, the slow-release effect of the polyesters-5 / sodium hyaluronate is absent, the oil control components are rapidly lost, and the enzyme activity cannot be effectively inhibited; and as shown in Comparative Example 5, the process affects the uniformity of the components, and the enzyme inhibition effect is affected. Therefore, the radix Stephaniae tetrandrae extract, radix Anemone vitifolii polysaccharide / fermentation broth, active ingredient and the corresponding preparation process used in the present application are helpful to improve the oil control effect of the makeup fixing spray product.

[0133] 5. Skin moisture content (MMV) test

[0134] Randomly select 45 subjects aged 18 to 30 years old without skin diseases, avoid using all types of skin care cosmetics and drugs two weeks before the experiment, on the experimental day, the subjects sit in an environment with a temperature of 25±2℃ and a humidity of 50±5% for 30 minutes, then wash the test site with water under the guidance of the staff, mark a 5cmx5cm experimental range on the inner side of the front end of the double arms, and the staff evenly applies a certain amount of product to the test site. Set the site without applying any product as the blank control group. During the test period, the subjects shall not apply any other cosmetics, skin care products or drugs. The measurement period is 6 hours, and drinking water, eating and strenuous exercise are prohibited during the test period. The staff selects the time points before application, 1 hour, 6 hours and 24 hours after application, and uses the skin moisture content tester Corneometer CM 825 to test the skin moisture content after spraying the product. Each site is measured 5 times, and the average value is selected.

[0135] Table 3 Change in skin water content (MMV / %)

[0136]

[0137]

[0138] From Table 3, after spraying the makeup fixing spray of the present application, the skin moisture content is increased after 1h, and the skin water content can still be maintained after 6h and 24h, the 24h skin moisture retention rate is all more than 99%, the 6h MMV is still maintained at more than 35%, proving that the active ingredients can repair the skin barrier and reduce water loss; in Comparative Example 3, the 24h MMV is only 33.21%, and the retention rate is 94.75%, which is lower than that of Example 1, because the fermentation liquor (promoting FLG synthesis) and sodium hyaluronate (water locking) are lacking, the skin barrier cannot be repaired, and the water is easily lost; in Comparative Example 5, the 1h MMV is 36.86%, but the 24h MMV decreases to 34.15%, because the process is improper, leading to large particle size of the oil phase, the moisturizing ingredients are released in advance, and the moisturizing time is shortened. Therefore, the makeup fixing spray of the present application has certain moisturizing and water locking effects, can make the skin maintain water and oil balance for a long time, and is suitable for long-time makeup fixing effect.

[0139] 6. Anti-ultraviolet and anti-blue light ability

[0140] The ultraviolet absorption ability of the makeup fixing products prepared in Example 1 and Comparative Examples 1-5 was determined by ultraviolet spectrophotometry, and the ultraviolet / blue light absorption rate was calculated by formula: (1-transmittance) x 100%.

[0141] The above test was repeated three times, the average value was taken, a graph was drawn, and the results are as follows Figure 2 、 Figure 3 .

[0142] As can be seen from Figure 2 , the makeup fixing product of the present application can cover the daily ultraviolet damage band and has certain anti-ultraviolet performance, while in Comparative Examples 1-3, the lack of active ingredients has a more significant influence on the anti-ultraviolet ability; and in Comparative Example 4, the 3-o-ethyl ascorbic acid is easily degraded by ultraviolet light due to the lack of microcapsule wrapping, reducing the UVB protection effect; the absorption rate of each band in Comparative Example 5 is close to that in Comparative Example 1, proving that the O / W / O structure is formed, and the process such as homogeneous and injection pump speed influences the dispersion of the protective ingredients.

[0143] As can be seen from Figure 3 , the makeup fixing product of the present application has certain absorption rate in the blue light band and can penetrate the skin by blue light; and as can be seen from Comparative Examples 1-3, the powder aconite extract (containing flavones) and the fermentation liquor (containing polysaccharides) can synergistically absorb blue light, and the fermentation liquor contributes more significantly (Comparative Example 2 is lower than Comparative Example 1); the anti-blue light of the makeup fixing product of the present application is achieved at the same time without introducing high-concentration chemical sunscreen agents, avoiding the risk of irritation and meeting the needs of sensitive skin.

[0144] 7. Anti-inflammatory ability and moisturizing ability

[0145] After the LPS medium with a mass concentration of 0.006 g / L is incubated for 6 h, the makeup fixing product prepared in Example 1 and Comparative Examples 1-5 is added and incubated for 24 h, and the contents of IL-1, IL-6, FLG and AQP3 and the like are detected by using an ELISA kit;

[0146] IL-1 - a secreted inflammatory factor, which induces local inflammation;

[0147] IL-6 - a bound inflammatory factor, which induces inflammatory response;

[0148] FLG - a skin barrier protein molecule, which maintains the skin barrier;

[0149] AQP3 - a skin moisturizing molecule, which has the skin barrier moisturizing effect;

[0150] The test results are shown in Table 1. Figure 4 It can be seen that the makeup fixing product prepared in the present application has an inhibitory effect on the contents of IL-1 and IL-6, indicating that it has an anti-inflammatory effect, and the contents of the barrier moisturizing related proteins FLG and AQP3 can be maintained to a certain extent, which has the moisturizing effect on the skin barrier and helps to improve the skin friendliness of the makeup fixing spray product. It can be seen from the test results of the comparative examples that the use of the extract of radix stephaniae tetrandrae, the polysaccharide / fermentation broth of radix comphoric, the active ingredients and the combination of the corresponding preparation process can significantly improve the anti-inflammatory effect and skin friendliness of the makeup fixing product, and show a synergistic effect. The anti-inflammatory and moisturizing ability of Comparative Example 2 is poorer than that of Comparative Example 1, indicating that the polysaccharide / fermentation broth of radix comphoric has a great contribution to the anti-inflammatory ability and moisturizing ability.

[0151] 8. Anti-migration and rubbing resistance test;

[0152] The lipstick is evenly applied on the arm of the subject, then the makeup fixing spray prepared in the present application is evenly sprayed, dried to form a film, and the arm is rubbed with a paper towel (each time back and forth), and the rubbing is performed 10 times at intervals of 15 minutes, and the test is performed for 3 times, and the anti-migration ability of the makeup fixing spray is evaluated by color difference Delta E, and the results are shown as follows:

[0153] Table 4 Anti-migration test results (color difference Delta E)

[0154] First time Second time Third time Example 1 0.24 0.29 0.34 Example 2 0.23 0.28 0.33 Example 3 0.24 0.28 0.35 Example 4 0.22 0.27 0.34 Comparative Example 2 0.28 0.33 0.38 Comparative Example 4 1.02 1.35 1.74 Comparative Example 5 1.14 1.52 1.83

[0155] As shown in Table 4, the smaller the color difference value Delta E, the better the anti-migration ability of the prepared makeup fixing spray. It can be seen that the prepared makeup fixing spray of the embodiment of the present application has good anti-migration ability, and the formed film has strong toughness and good adhesion; as shown in Comparative Example 2, the lack of fermentation liquor reduces the adhesion of the formed film to the skin; in Comparative Example 4, the lack of the bridging effect of polyester-5 / sodium hyaluronate makes the makeup film poorly fit the skin and easy to migrate after rubbing; and in Comparative Example 5, the film-forming property of the product is changed due to the different preparation process, and the migration is aggravated with the increase of the rubbing times, the film thickness is uneven due to the relatively large particle size of the oil phase, and the anti-migration ability is slightly poor.

[0156] 9. Sweat and water resistance test

[0157] The artificial sweat solution (sodium chloride 1.0%, urea 0.5%, lactic acid 0.5%, pH adjusted to 4.5-5.5) was prepared, and the makeup fixing spray was sprayed on a glass slide (film-forming area 2 cm x 2 cm), and then dried, and then immersed in the artificial sweat solution and deionized water respectively, and placed in a constant temperature incubator at 37°C for 2 h; after taking out and drying, the integrity of the makeup film (no cracks and no peeling) was observed by an optical microscope, and the water contact angle after film formation was measured (the larger the contact angle, the better the water resistance), and the results are shown as follows:

[0158] Table 5. Sweat and water resistance test

[0159]

[0160]

[0161] As shown in Table 5, the water contact angle of Example 1 of the present application is the highest, and the film is complete and intact, which proves that a dense and hydrophobic protective film is formed. The film of Comparative Example 4 has local cracking, and the contact angle is significantly reduced, and the water resistance is poor; and the change in the process of Comparative Example 5 also affects the film-forming property of the product, thereby affecting other properties; it is shown that the O / W / O structure formed by microencapsulation can form a strong micro-mesh film on the surface of the skin, and thus the sweat and water resistance makeup function can be realized.

[0162] 10. Stability test

[0163] 40°C high temperature storage: the sample was sealed in a transparent glass bottle and placed in a 40°C constant temperature incubator, and after 3 months, the layering was observed (layering rate = lower liquid volume / total sample volume x 100%);

[0164] -10°C low temperature cycle: the sample was first frozen at -10°C for 24 h, and then thawed at room temperature (25°C) for 2 h, and the cycle was repeated for 5 times, and whether crystallization and layering occurred was observed;

[0165] Centrifugal stability: the sample was placed in a centrifuge tube, centrifuged at 3000 r / min for 10 min, and the layering was observed.

[0166] The results are as follows:

[0167] Table 6 stability test

[0168]

[0169]

[0170] As can be seen from Table 6, the product of Example 1 of the present application shows excellent stability (only 0.8% of layering, no crystallization and no layering) under high temperature, low temperature and centrifugal test. Although the stability of Comparative Example 4 and Comparative Example 5 is better than that of Comparative Example 3, they still show slight layering, which proves that the microencapsulation process and the overall process of the product of the present application are crucial for maintaining the stability of the product during the shelf life.

[0171] Therefore, as can be seen from the above, the makeup fixing spray product prepared by the present application has certain antioxidant, oil control, moisturizing and water locking, film forming and stability. The active ingredients of the powder of Stephania tetrandra S. Moore extract, radix comfrey polysaccharide / fermentation broth and the active ingredients containing both are complementary in antioxidant, anti-inflammatory, oil control, repair and other aspects, and have synergistic effect. The process is also indispensable. The O / W / O microencapsulation process is the core key technology to realize efficient delivery of active substances, long-lasting makeup, and product stability. The components and process complement each other, greatly improve the anti-ultraviolet, anti-blue light, anti-inflammatory, skin-friendly and film forming, stability performance of the makeup fixing product, and comprehensively improve the excellent performance of the makeup fixing product to meet the makeup fixing demand.

Claims

1. A method of improving the skin feel and stability of a setting spray, characterized in that, It comprises the following steps: 1) Oil-in-water (O / W) treatment: hydrophilic emulsifier is added to the water phase mixture, heated, dissolved, stirred and uniformly obtained emulsion, the inner oil phase is heated, dissolved, slowly added to the emulsion, then PEG-40 hydrogenated castor oil is added, stirred and uniformly dispersed, and then ultrasonic treatment is performed, thereby obtaining O / W emulsion; The homogenization dispersion is performed at a rotation speed of 2000-3500 r / min for 2-5 min; The ultrasonic treatment is performed at a power of 300-400 W for 10-20 min; 2) O / W / O microencapsulation treatment: lipophilic emulsifier is added to the outer oil phase, heated, dissolved, slowly and uniformly, and then microencapsulation agent is added at a mass ratio of (1-1.5):(3-4):(1.5-2.5), homogenization dispersion is performed at a rotation speed of 800-1000 r / min for 8-10 min, then ultrasonic treatment is performed at a power of 100-200 W for 5-10 min, and then the temperature is reduced to normal temperature, then the O / W emulsion is slowly added to the outer oil phase at a volume ratio of (4-5):(5-6) by using a syringe pump, homogenization dispersion is performed at a rotation speed of 3000-5000 r / min for 3-6 min, thereby obtaining O / W / O microencapsulation product, i.e. makeup fixing product, and the makeup fixing product is used by a sprayer, thereby obtaining makeup fixing spray. The hydrophilic emulsifier in step 1) comprises glycerol polyether-26, cetylstearyl polyether-12, cetylstearyl polyether-20 and sucrose stearate at a mass ratio of (3-4):(1-1.6):(3-4):(1-1.2).

2. The method of claim 1, wherein the method is characterized by, The water phase mixture in step 1) is prepared by mixing water, active ingredient, glycerol, ethylhexylglycerin, p-hydroxyacetophenone, nicotinamide and disodium EDTA, stirring and uniformly, at a stirring temperature of 50-60 ℃ and a stirring speed of 200-300 r / min, thereby obtaining the water phase mixture.

3. The method of claim 1, wherein the method is characterized by, The active ingredient accounts for 10-15% by mass fraction, the glycerol accounts for 25-35%, the ethylhexylglycerin accounts for 5-10%, the p-hydroxyacetophenone accounts for 0.5-1%, the nicotinamide accounts for 2-5%, the disodium EDTA accounts for 2-5%, and water accounts for 100%. The active ingredient is obtained by previously dissolving Hamamelis virginiana water, Gentiana scabra extract, Stephania tetrandra extract, Cynanchum paniculatum polysaccharide / fermentation broth and 3-o-ethyl ascorbic acid in an organic solution of ethanol and butanediol.

4. The method of claim 3, wherein the method is characterized by, ​ The active ingredients include 5-15% of water of Hamamelis virginiana, 1-1.5% of Gentiana scabra extract, 10-20% of Stephania tetrandra extract, 5-10% of Macrotomia sieboldi polysaccharide / fermentation liquor, 10-20% of 3-o-ethyl ascorbic acid, 15-25% of ethanol, and 20-35% of butanediol.

5. The method of claim 4, wherein the method is characterized by, The Macrotomia sieboldi polysaccharide / fermentation liquor is obtained by inoculating activated Bacillus siamensis into a Macrotomia sieboldi polysaccharide aqueous solution, fermenting at 35℃, and then evaporating water to obtain. The inoculation concentration of the Paenibacillus thiamma is 1.5×10 7 ~2×10 7 CFU / mL polysaccharide aqueous solution, and the concentration of the polysaccharide aqueous solution of the Eustoma grandiflorum is 15~20%. The preparation of the Macrotomia sieboldi polysaccharide includes: a) extraction: the Macrotomia sieboldi material is extracted by hot water, precipitated by ethanol, and purified by centrifugation to obtain crude polysaccharide of Macrotomia sieboldi; b) purification: the crude polysaccharide of Macrotomia sieboldi is extracted by hot water, precipitated by ethanol, centrifuged, and then purified by DEAE-52 cellulose column chromatography and freeze-drying to obtain Macrotomia sieboldi polysaccharide with a purity of ≥90%.

6. The method of claim 1, wherein the method is characterized by, The hydrophilic emulsifier in step 1) is added in an amount of 2-6wt% of the water phase mixture. The inner oil phase is added in an amount of 3-6wt% of the emulsion. The PEG-40 hydrogenated castor oil is added in an amount of 2-3wt% of the emulsion.

7. The method of claim 1, wherein the method is characterized by, The inner oil phase in step 1) includes one or more of tocopheryl acetate and cetearyl isononanoate.

8. The method of claim 1, wherein the method is characterized by, The outer oil phase in step 2) includes a mixture of tocopheryl acetate, dimethicone / vinyl dimethicone crosspolymer, and silica, wherein the tocopheryl acetate accounts for 75-85wt%, the dimethicone / vinyl dimethicone crosspolymer accounts for 10-15wt%, and the silica accounts for 5-8wt%.

9. The method of claim 1, wherein the method is characterized by, The lipophilic emulsifier in step 2) includes cetearyl isononanoate, cetearyl stearate, and cetyl palmitate, wherein the cetearyl isononanoate accounts for 25-40wt%, the cetearyl stearate accounts for 25-40wt%, and the cetyl palmitate accounts for 25-50wt%.

10. The method of claim 1, wherein the method is characterized by, The microencapsulating agent in step 2) is prepared from polyester-5, cetearyl alcohol, and sodium hyaluronate, and the preparation process includes: mixing 15-25% of polyester-5 and 2-5% of sodium hyaluronate, heating to 80-100℃, stirring uniformly, and then adding 75-85% of cetearyl alcohol for high-speed homogenization and dispersion.

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