Self-adaptive pipetting method and pipetting device based on duration feedback

Abstract

The invention discloses a self-adaptive pipetting method and device based on duration feedback, and belongs to the technical field of liquid transfer. The invention relates to a self-adaptive pipetting method and a pipetting device based on time length feedback, which are characterized in that the pipetting device is used for quantitatively transferring and conveying liquid; comprising the following steps: emptying residual liquid in a target container; the pump is controlled to extract liquid from the source container, the liquid is conveyed to the target container through the connecting pipeline, and the liquid level descending duration fed back by the liquid level sensor is recorded through a timing module of a controller electrically connected with the pump; calculating the transfer time of the corresponding unit liquid volume based on the liquid level falling time of the liquid level sensor between the adjacent scales, further calculating the transfer speed, and determining the total duration required for reaching the target transfer volume according to the transfer speed; and when the timing of the timing module reaches the total duration, the operation of the pump is stopped, so that the quantitative transfer of the liquid is completed.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of liquid transfer, and more particularly relates to a self-adaptive pipetting method based on time length feedback and a pipetting device. BACKGROUND

[0002] If a certain amount of liquid (including water, gasoline, oil, medicine, chemical agent, etc.) is to be transferred from a source container to a target container (such as a tank for storing engine oil on a car) without supervision, in the prior art, generally, a target container is designed, and the capacity of the target container is a multiple of the capacity of the source container; at this time, only the entire liquid of several source containers needs to be transferred, and the task can be completed; the disadvantage of this method is that different specifications of target containers require different specifications of source containers, the source containers have poor compatibility, and the cost of designing and producing multiple models of assembly lines is high; in other methods, a flow meter is also used to achieve this, and the problem of this method is that the flow meter is high in cost; in view of the above problems, it is necessary to provide a method and device which are simple in structure, convenient to carry, low in manufacturing cost and capable of stably transferring a specified amount of liquid to meet the needs of different use scenarios. SUMMARY

[0003] The technical problem to be solved by the application is to provide a self-adaptive pipetting method based on time length feedback and a pipetting device, which can realize quantitative transfer of liquid.

[0004] The self-adaptive pipetting method based on time length feedback and the pipetting device are used to transfer a specified amount of liquid to a target container, and include the following steps:

[0005] S1, providing a pipetting device, the pipetting device including a source container having a cavity, a first liquid conveying path in communication with a target container, a pump arranged on the first liquid conveying path, a second liquid conveying path in communication with the source container and the first liquid conveying path, a third liquid conveying path in communication with an externally arranged transition container and the first liquid conveying path, and a first controller electrically connected with the pump; the source container is internally provided with a liquid level sensor for monitoring the liquid level in the source container and outputting a liquid level signal;

[0006] S2, before pipetting, the pump is driven to run by the first controller, residual liquid in the target container is conveyed to an externally arranged transition container through the first liquid conveying path and the third liquid conveying path, so that the target container is in an empty state;

[0007] S3, when the target container is in the empty state, the running direction of the pump is changed by the first controller, and liquid is transferred from the source container to the target container through the first liquid conveying path and the second liquid conveying path;

[0008] S4, the liquid level sensor detects the liquid level in the source container drops and outputs an electrical signal, the first controller calculates the time required for the transfer of a unit volume of liquid according to the corresponding time length of the liquid level change, and determines the total pipetting time based on the calculation result;

[0009] S5, the first controller drives the pump to run, and stops the operation of the pump after reaching the total pipetting time, thereby realizing the control of the volume of the transferred liquid.

[0010] As a further improvement of the application, the liquid level sensor is provided with uniform scales in the vertical direction, which respectively represent different gears, and the volume of liquid corresponding to the adjacent two gears is fixed and the same;

[0011] In step S4, when the liquid level drops from one gear on the liquid level sensor to the next gear on the liquid level sensor, the liquid level sensor feeds back a new electrical signal, and at the same time, the timing module of the corresponding first controller records the time, so as to obtain the time length required for the liquid level of the adjacent two gears on the liquid level sensor to drop, and thus according to the volume of liquid corresponding to the adjacent two gears and the time length required for the liquid level of the two gears to drop, the speed of the liquid transfer process is obtained, and thus the total time required to reach the target transfer volume is obtained.

[0012] As a further improvement of the application, the gears of the liquid level sensor are N i , i = 0, 1, 2, …; the volume of liquid corresponding to the adjacent two gears is ΔV;

[0013] In step S4, the height of the liquid in the source container continues to drop as the liquid is extracted, and when the first new gear N i is reached, the time t K is recorded; when the second new gear N i-1 is reached, the time t K-1 is recorded.

[0014] From N i to N i-1 is a standard gear, at this time, the volume of liquid between N i-1 and N i is ΔV;

[0015] The time required for the liquid to drop from the lowest gear to the empty gear, i.e. the time t1 required from the last gear to the completion of the extraction of the liquid, is calculated by the formula:

[0016]

[0017] The time required to fill the entire target container is:

[0018]

[0019] Wherein, m=2, 3, …, K; K≥2.

[0020] A pipetting device for any of the above-mentioned pipetting methods based on time length feedback, comprising a first controller, a source container, a lid assembly and a pump, the source container has a cavity for containing liquid and an inlet and outlet in communication with the outside; the lid assembly is detachably connected to the inlet and outlet of the source container and is in communication with the cavity of the source container; the input end of the pump is in communication with the lid assembly, and the output end of the pump is used to communicate with the target container of the external device.

[0021] The first controller is electrically connected to the pump, and a timing module is arranged on the first controller to control the running time of the pump, so as to realize the transfer of a specified amount of liquid.

[0022] As a further improvement of the application, the lid assembly comprises a cover, the bottom of the cover is fixedly provided with a joint portion, the joint portion is hollowly provided with a cavity, and the cavity is in communication with the cavity of the source container to form a container cavity.

[0023] As a further improvement of the application, the lid assembly further comprises a second controller; the lower end of the liquid level sensor penetrates the cover and extends into the cavity of the source container, and is used to monitor the liquid level information in the cavity of the source container.

[0024] The liquid level sensor and the second controller are electrically connected and driven and controlled by the second controller.

[0025] As a further improvement of the application, the top of the lid assembly is provided with an oil nozzle; the input end of the oil nozzle is provided with a first connecting pipe, and the oil nozzle is in communication with the container cavity through the first connecting pipe; the output end of the oil nozzle is provided with a connecting pipeline, and the connecting pipeline is in communication with the input end of the pump.

[0026] The connecting pipeline comprises a third connecting pipe, a three-way pipeline and a fourth connecting pipe in sequence, and the three-way pipeline comprises a first connecting port, a second connecting port and a third connecting port.

[0027] The third connecting pipe is connected to the first connecting port of the three-way pipeline, and a first valve is arranged between the third connecting pipe and the three-way pipeline; the fourth connecting pipe is connected to the second connecting port of the three-way pipeline; and the end of the fourth connecting pipe away from the three-way pipeline is connected to the first connecting end of the pump.

[0028] The third connecting port of the three-way pipeline is connected with a sixth connecting pipe, which is used to be connected with a transition container arranged outside, and a second valve is arranged on the sixth connecting pipe.

[0029] The first valve and the second valve are both one-way valves, which are used to limit the flow direction of the liquid.

[0030] As a further improvement of the present application, the cover and the handle are further included; the handle is fixedly arranged on the top of the source container; the cover is arranged on the top of the source container and forms an opening for easy gripping at the position of the handle.

[0031] As a further improvement of the present application, the cover includes a first cover and a second cover arranged opposite and side by side, the pump is arranged in the first cover, and the first controller is arranged in the second cover.

[0032] As a further improvement of the present application, the adjacent ends of the first cover and the second cover are provided with through grooves, and the two through grooves form an opening of the hollow structure.

[0033] The adjacent ends of the first cover and the second cover provided with the through grooves are detachably connected and arranged on the two ends of the handle and are mounted and connected with the two ends of the handle.

[0034] Compared with the prior art, the present application has the following beneficial effects:

[0035] The liquid level sensor is provided with uniform scales in the vertical direction, and adjacent gears correspond to fixed volumes. The liquid flow rate can be obtained by calculating the time length of the gear drop, and the high-precision control of the transferred liquid volume is realized by combining the flow stability of the pump in a short time. The transfer time of the unit volume of liquid is obtained by detecting the change of the liquid level in the source container by the liquid level sensor and feeding back and calculating the time length of the liquid level drop by the timing module, so that the accurate control of the liquid transfer amount is realized without using the flow meter, and the system cost is greatly reduced.

[0036] The flexible adjustment of the liquid transfer amount of different target containers can be realized by the time length feedback mode, without the need to design different specifications of the source container for different container volumes, thereby improving the universality and compatibility of the device.

[0037] During use, the target container is only connected with the pump output end, the controller can automatically drive the pump to run according to the preset time length and automatically stop when the operation is completed, without manual intervention, thereby reducing the operation difficulty and improving the use experience.

[0038] The structure is simplified and convenient to carry: the combination design of the source container, the cover assembly, the pump and the controller avoids the dependence on complex pipeline arrangement and additional sensors, and the overall structure is simple, convenient to assemble, carry and maintain. BRIEF DESCRIPTION OF DRAWINGS

[0039] Figure 1 It is a perspective view of the device of the present application;

[0040] Figure 2 It is a structural schematic view of the device of the present application without the cover;

[0041] Figure 3 It is a sectional view of the device of the present application;

[0042] Figure 4 This is a schematic diagram of the device drive conveying path structure of the present invention.

[0043] Explanation of the labels in the diagram:

[0044] 1. Source container; 11. Connecting post; 2. Lid assembly; 21. Cover; 22. Connecting post; 23. Mounting slot; 24. Liquid level sensor; 241. Float; 3. Pump; 31. First connecting end; 32. Second connecting end; 4. Nozzle; 51. First connecting pipe; 52. Second connecting pipe; 53. Third connecting pipe; 54. T-connector; 55. Fourth connecting pipe; 56. Fifth connecting pipe; 57. First valve; 58. Second valve; 59. First filter element; 6. Cover; 61. First cover; 62. Second cover; 7. Handle. Detailed Implementation

[0045] The present invention will be further described in detail below with reference to the embodiments. The following embodiments are explanations of the present invention, but the present invention is not limited to the following embodiments.

[0046] Specific Implementation Example 1: Please refer to... Figure 1 - Figure 4 An adaptive pipetting method based on time-feedback,

[0047] The liquid is transferred using a device for transferring a specified amount of liquid;

[0048] The device for transferring a specified amount of liquid includes a source container 1 and a pump 3. The source container 1 is hollow and has a cavity. The top of the source container 1 has an inlet and outlet. A hollow connecting column 11 is fixedly installed on the outer periphery of the top of the inlet and outlet. A lid assembly 2 is detachably installed on the connecting column 11.

[0049] The outer periphery of the lower end of the lid assembly 2 is threadedly connected to the inner wall of the connecting post 11.

[0050] The lid assembly 2 includes a cap 21; a hollow connecting post 22 is fixedly provided at the bottom of the cap 21; the connecting post 22 is surrounded by threads that match the threads surrounding the connecting post 11, and the connecting post 22 and the connecting post 11 are threadedly connected; the connecting post 22 is hollow and has a cavity, and the bottom of the connecting post 22 is open through to connect the cavity of the connecting post 22 with the cavity inside the source container 1 to form a cavity.

[0051] The top of the cover 21 is fixedly provided with an oil nozzle 4, the input end of the oil nozzle 4 is fixedly provided with a first connecting pipe 51, the end of the first connecting pipe 51 away from the oil nozzle 4 penetrates the cover 21 and extends into the cavity; the end of the first connecting pipe 51 away from the oil nozzle 4 is fixedly provided with a second connecting pipe 52, the end of the second connecting pipe 52 away from the first connecting pipe 51 extends into the bottom of the cavity of the source container 1, so as to transfer the liquid in the source container 1 to the outside; the output end of the oil nozzle 4 is fixedly provided with a third connecting pipe 53; the end of the third connecting pipe 53 away from the oil nozzle 4 is mounted with a three-way pipe 54; the three-way pipe 54 includes a first connecting port, a second connecting port and a third connecting port;

[0052] The third connecting pipe 53 is fixedly connected with the first connecting port of the three-way pipe 54, and a first valve 57 is mounted between the third connecting pipe 53 and the three-way pipe 54; the second connecting port of the three-way pipe 54 is fixedly connected with a fourth connecting pipe 55, the end of the fourth connecting pipe 55 away from the three-way pipe 54 is mounted with a first connecting end 31 of the pump 3, and the second connecting end 32 provided at the end of the pump 3 away from the first connecting end 31 is fixedly connected with a fifth connecting pipe 56; the end of the fifth connecting pipe 56 away from the pump 3 is used for connecting a target container for transferring liquid.

[0053] The third connecting port of the three-way pipe 54 is mounted with a sixth connecting pipe for mounting connection with a transition container provided outside, and a second valve 58 is mounted on the sixth connecting pipe, so as to control the flow direction of the liquid.

[0054] Preferably, the pump 3 is a bidirectional pump capable of bidirectional fluid transmission.

[0055] Preferably, the first valve 57 and the second valve 58 are both one-way valves, so as to control the flow direction of the liquid.

[0056] Optionally, a filter 59 is mounted on the third connecting pipe 53 and the fifth connecting pipe 56, so as to filter the passing liquid.

[0057] The top of the cover 21 is fixedly provided with a mounting groove 23, and a cover plate is arranged on the top of the mounting groove 23;

[0058] The kettle cover assembly 2 further includes a liquid level sensor 24 and a second controller, the liquid level sensor 24 includes an electronic tube, one end of the electronic tube sleeved with a float 241 penetrates the cover 21 and extends into the cavity in the source container 1 together with the float 241, and the top of the liquid level sensor 24 is mounted in the mounting groove 23 on the top of the cover 21; the liquid level sensor 24 is electrically connected with the second controller and is driven and controlled by the second controller; the second controller is embedded in the mounting groove 23.

[0059] The liquid level sensor 24 is a prior art, which will not be described here.

[0060] The basic working principle of the liquid level sensor 24: the change of the liquid level makes the float 241 slide up and down along the electronic tube, and the magnet inside the float 241 drives the reed switch arranged at different positions in the electronic tube to change the electronic signal of the internal circuit in real time, and then the change is fed back to the first controller arranged outside through the wire.

[0061] The device further comprises a cover 6 covering the top of the source container 1; and the kettle cover assembly 2 is arranged independently of the cover 6.

[0062] The top of the source container 1 is fixedly provided with a handle 7; the cover 6 comprises a first cover 61 and a second cover 62; the first cover 61 and the second cover 62 are arranged opposite and side by side, and the adjacent ends of the first cover 61 and the second cover 62 are detachably connected, and the connection part of the two is fixed to the handle 7.

[0063] Specifically, one end of the first cover 61 covers the top of the two ends of the handle 7, one end of the second cover 62 covers the top of the connection part of the first cover 61 and the handle 7, and the ends of the first cover 61 and the second cover 62 towards the handle 7 are provided with through grooves, and the two through grooves form an open part of a hollow structure to expose the handle 7, so that the operator can smoothly operate the handle 7.

[0064] The pump 3 is installed in the first cover 61.

[0065] The device further comprises a first controller installed in the second cover 62, the pump 3 and the first controller are electrically connected and conductive, and are driven and controlled by the first controller; and the first controller is provided with a timing module to monitor the time of liquid transfer.

[0066] The device realizes accurate control of the transfer amount in a single liquid transfer process through the cooperation of the source container 1, the kettle cover assembly 2, the liquid level sensor 24, the pump 3 and the two controllers.

[0067] Optionally, the fourth connecting pipe 55 and the fifth connecting pipe 56 constitute a first liquid conveying path, the pump 3 is arranged on the first liquid conveying path; the first connecting pipe 51, the second connecting pipe 52 and the third connecting pipe 53 constitute a second liquid conveying path; and the sixth connecting pipe constitutes a third liquid conveying path; and the first liquid conveying path, the second liquid conveying path and the third liquid conveying path are connected through the three-way pipe 54.

[0068] The source container 1 pre-stores the liquid to be transferred, the kettle cover assembly 2 is installed at the top inlet and outlet of the source container 1, the pump 3, the oil nozzle 4 on the cover 21 and each connecting pipe in communication with the pump 3 and the oil nozzle 4 constitute a liquid conveying path, and the pump 3 is in communication with the corresponding container through the connecting pipe.

[0069] Before use, the liquid in the target container is emptied, the fifth connecting pipe 56 is connected with the target container, the pump 3 is started, the liquid in the target container is once passed through the fifth connecting pipe 56, the pump 3, the fourth connecting pipe 55, the third connecting port of the three-way pipe 54, and then enters the sixth connecting pipe, and is transferred to the transition container through the sixth connecting pipe;

[0070] After the liquid in the target container is emptied, the running time of the pump 3 is preset by the first controller, and then the first controller drives the pump 3 to start and change the running direction of the pump 3, the pump 3 draws the liquid from the cavity of the source container 1 through the connecting pipe and the oil nozzle 4, sequentially passes through the third connecting pipe 53, the three-way pipe 54, the fourth connecting pipe 55, the pump 3 and the fifth connecting pipe 56, and finally is transported into the target container connected with the fifth connecting pipe 56;

[0071] The first controller runs the pump 3 according to the preset time length of the timing module, and automatically turns off the power supply of the pump 3 at the end of the timing, so as to realize one-time transfer of a predetermined volume of liquid; in a very short time, the liquid transfer speed of the pump 3 is basically stable, so the amount of the transferred liquid is certain within the predetermined time, so the quantitative transfer of the liquid can be realized;

[0072] The liquid level sensor 24 is installed on the lid assembly 2, the electronic tube and the float 241 of which extend into the cavity of the source container 1, and can detect the liquid level height of the liquid in the cavity in real time; the float 241 moves up and down along the electronic tube with the change of the liquid level, and drives the reed switch at different positions in the electronic tube to act through the magnet inside the float 241, so that the liquid level signal is converted into an electric signal and transmitted to the second controller through the wire;

[0073] The flow stability of the pump 3 in a short time is utilized to realize accurate metering transfer without a real-time flow meter, reduce the structural complexity and cost, and improve the transfer accuracy and operation simplicity.

[0074] The first controller and the second controller are electrically connected and conducted, the liquid level sensor 24 converts the liquid level signal into an electric signal and transmits it to the second controller through the wire, and then feeds back to the first controller through the second controller.

[0075] The liquid level sensor 24 is provided with uniform scales, which represent different gears N i (i=0, 1, 2, …); the volume of the liquid corresponding to the adjacent two gears is fixed and the same, which is ΔV;

[0076] It is known that the static lift of the pump 3 increases when the liquid level decreases, and the total lift of the pump 3 also increases, and the calculation formula is:

[0077] H=H0-k·Q 2

[0078] H: actual head (unit: m), i.e. the height that the pump can lift water, including water level difference and pipeline loss;

[0079] H0: maximum head at zero flow rate;

[0080] k: resistance coefficient (pipeline friction, elbow resistance, etc.);

[0081] Q: flow rate (unit: m 3 / s or m 3 / h).

[0082] The water pump power formula can be transformed into a direct relationship between flow rate and head:

[0083]

[0084] Therefore, for the same pump transporting the same uniform fluid, the flow rate only changes with the head, and the two are inversely proportional;

[0085] When the volume of the liquid in the source container 1 is i·ΔV (where i is the total number of gears, and ΔV is the volume of each gear), the time for the liquid level to drop from the previous gear to the next gear is t m ; Set the time sequence of t m as t K , t K-1 , …, t2; The time sequence of liquid reaching N i , N i-1 , …, N1;

[0086] t K corresponds to the time when the liquid reaches the gear N i , t K-1 corresponds to the time when the liquid reaches the gear N i from the gear N i-1 , …, t2 corresponds to the time when the liquid reaches the gear N1 from the gear N2;

[0087] All t m are proportional to , and the proportional constant d satisfies the following conditions:

[0088]

[0089] Therefore, the time for the liquid to drop from the gear N1 when the liquid level is at the lowest to the empty gear N0 is the time sequence t1, i.e. the time t1 required when the last gear is reached and the liquid is pumped out, and the calculation formula is:

[0090]

[0091] K: the maximum index of the known time sequence.

[0092] For example, if t5, t4, t3, t2 are known (i.e. K = 5), then:

[0093]

[0094] The pipetting method is as follows:

[0095] S1: Empty the liquid in the target container: the fifth connecting pipe 56 is connected to the target container, the pump 3 is started, and the liquid in the target container is once passed through the fifth connecting pipe 56, the pump 3, the fourth connecting pipe 55, the third connecting port of the three-way pipe 54, and then enters the sixth connecting pipe, and then enters the externally arranged transition container, so that the liquid in the target container is transferred to the transition container;

[0096] The remaining liquid in the target container is pumped out by the pump 3 and the connecting pipes;

[0097] S2: Start transferring the liquid, and record the corresponding time through the timing module arranged on the first controller which is electrically connected to the pump 3;

[0098] The height of the liquid in the source container 1 continues to decrease as the liquid is pumped out, and when it reaches the first new gear N i , the time t K is recorded; when it reaches the second new gear N i-1 , the time t K-1 is recorded;

[0099] From N i to N i-1 , it is a standard gear, at this time, the liquid volume between N i and N i-1 is ΔV, that is, the liquid volume between two adjacent gears is ΔV, which is recorded as a standard gear volume;

[0100] Then, the time required for the liquid to drop from the lowest gear to the empty gear, that is, the time t1 required from the last gear to the end of the liquid pumping, is calculated as follows:

[0101]

[0102] When the liquid in the source container 1 is transferred to the target container, the total time required is t total , and the calculation formula is:

[0103]

[0104] Wherein, m = 2, 3, …, K; K ≥ 2.

[0105] S3: When the timing module timing reaches ttotal When the target volume has been filled with the required amount of liquid, the operation of the pump 3 is stopped to stop the transfer of liquid; at this point, the transfer of the required amount of liquid into the target volume has been completed.

[0106] The above description is merely preferred embodiments of the present application, but not the only ones; any person skilled in the art should understand that, within the technical scope disclosed by the present application, any equivalent replacement or change according to the technical solution and the improvement concept of the present application should be covered by the protection scope of the present application.

Claims

1. A time-based feedback adaptive pipetting method for transferring a quantified volume of liquid into a target container, characterized in that: The method comprises the following steps: S1, providing a pipetting device, the pipetting device comprising: a source container (1) having a cavity, a first liquid conveying path in communication with a target container, a pump (3) arranged on the first liquid conveying path, a second liquid conveying path in communication with the source container (1) and the first liquid conveying path, a third liquid conveying path in communication with an externally arranged transition container and the first liquid conveying path, and a first controller electrically connected with the pump (3); the source container (1) is internally provided with a liquid level sensor (24) for monitoring the liquid level in the source container (1) and outputting a liquid level signal; S2, before pipetting, the pump (3) is driven to operate by the first controller, and residual liquid in the target container is conveyed to an externally arranged transition container through the first liquid conveying path and the third liquid conveying path, so that the target container is in an empty state; S3, when the target container is in the empty state, the running direction of the pump (3) is changed by the first controller, and liquid is transferred from the source container (1) to the target container through the first liquid conveying path and the second liquid conveying path; S4, the liquid level sensor (24) detects the decrease of the liquid level in the source container (1) and outputs an electrical signal, the first controller calculates the time required for transferring a unit volume of liquid according to the time length corresponding to the change of the liquid level, and determines the total pipetting time based on the calculation result; S5, the pump (3) is driven to operate by the first controller, and the operation of the pump (3) is stopped after the total pipetting time is reached, so as to control the volume of the transferred liquid.

2. The method of claim 1, wherein: The liquid level sensor (24) is provided with uniform scales in the vertical direction, which represent different gears respectively, and the volume of liquid corresponding to the adjacent two gears is fixed and the same; In step S4, when the liquid level drops from one gear on the liquid level sensor (24) to the next gear on the liquid level sensor (24), the liquid level sensor (24) feeds back a new electrical signal, and the timing module of the corresponding first controller records the time, so as to obtain the time length required for the liquid level of the adjacent two gears on the liquid level sensor (24) to drop, and thus the speed of the liquid transfer process is obtained according to the volume of liquid corresponding to the adjacent two gears and the time length required for the liquid level of the two gears to drop, so as to obtain the total time required to reach the target transfer volume.

3. The method of claim 2, wherein: Gear of the liquid level sensor (24) is N i , i = 0, 1, 2, …; the volume of the liquid corresponding to the adjacent two gears is ΔV; In step S4, the level of the liquid in the source container (1) continues to drop as the liquid is extracted, reaching a first new level N i , at which time t K is noted; a second new level N i-1 , at which time t K-1 is noted; From N i Up to N i-1 The gear is a standard gear, at which point N... i-1 With N i The liquid volume between them is ΔV; The time required for the liquid to drop from the lowest gear to the empty gear is the time t1 required from the last gear to the completion of the liquid extraction, and the calculation formula is: The time required to fill the entire target container is: Wherein, m=2, 3, …, K; K≥2.

4. A pipetting device for use in any of the adaptive pipetting methods based on time- length feedback according to claims 1 to 3, characterized in that The pipetting device comprises a first controller, a source container (1), a lid assembly (2), and a pump (3). The source container (1) has a cavity for containing liquid and an inlet and outlet in communication with the outside. The lid assembly (2) is detachably connected to the inlet and outlet of the source container (1) and is in communication with the cavity of the source container (1). The input end of the pump (3) is in communication with the lid assembly (2), and the pump (3) is in communication with the cavity of the source container (1) through the lid assembly (2). The output end of the pump (3) is used to communicate with an externally arranged target container. The first controller is electrically connected with the pump (3) in conduction, and a timing module is arranged on the first controller. The running time of the pump (3) is controlled through the timing module, so as to realize the transfer of a specified amount of liquid.

5. A pipetting device according to claim 4, characterized in that: The kettle cover assembly (2) comprises a cover (21), and a joint portion is fixedly arranged at the bottom of the cover (21). The joint portion is hollowly provided with a chamber, and the chamber is in communication with the cavity of the source container (1) to form a containing cavity.

6. A pipetting device according to claim 5, characterized in that: The kettle cover assembly (2) further comprises a second controller; the lower end of the liquid level sensor (24) penetrates through the cover (21) and extends into the cavity of the source container (1), and is used for monitoring the liquid level information in the cavity of the source container (1). The liquid level sensor (24) and the second controller are electrically connected in conduction and are driven and controlled by the second controller.

7. A pipetting device according to claim 4, characterized in that: The top of the kettle cover assembly (2) is provided with an oil nozzle (4); the input end of the oil nozzle (4) is provided with a first connecting pipe (51), and is in communication with the containing cavity through the first connecting pipe (51); the output end of the oil nozzle (4) is provided with a connecting pipeline, and is in communication with the input end of the pump (3) through the connecting pipeline; The connecting pipeline comprises a third connecting pipe (53), a three-way pipe (54) and a fourth connecting pipe (55) which are sequentially communicated, and the three-way pipe (54) comprises a first connecting port, a second connecting port and a third connecting port. The third connecting pipe (53) is connected with the first connecting port of the three-way pipe (54), and a first valve (57) is arranged between the third connecting pipe (53) and the three-way pipe (54); the fourth connecting pipe (55) is connected with the second connecting port of the three-way pipe (54); and the end of the fourth connecting pipe (55) away from the three-way pipe (54) is connected with the first connecting end (31) of the pump (3). The third connecting port of the three-way pipe (54) is connected with a sixth connecting pipe for connecting with an externally arranged transition container, and a second valve (58) is arranged on the sixth connecting pipe. The first valve (57) and the second valve (58) are both one-way valves for limiting the flow direction of the liquid.

8. The pipetting device of claim 4, wherein: Further comprising a cover (6) and a handle (7); the handle (7) is fixedly arranged on the top of the source container (1); the cover (6) covers the top of the source container (1) and forms an opening at the position of the handle (7) for easy gripping.

9. A pipetting device according to claim 8, characterized in that: The cover (6) comprises a first cover (61) and a second cover (62) arranged opposite to each other, the pump (3) is arranged in the first cover (61), and the first controller is arranged in the second cover (62).

10. A pipetting device according to claim 9, characterized in that: The adjacent ends of the first cover (61) and the second cover (62) are provided with through grooves, and the two through grooves form an opening of a hollow structure; The adjacent ends of the first cover (61) and the second cover (62) provided with the through grooves are detachably connected and arranged on the two ends of the handle (7) and connected with the two ends of the handle (7).

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