Carbon nanotube bridging functionalized mesoporous MOFs dual-mode sensing device
By combining carbon quantum dot-doped carbon nanotubes to bridge metal-organic framework materials, an electrochemical and fluorescence sensor combination was achieved, solving the problem of insufficient sensitivity and accuracy in traditional sensors and realizing highly sensitive and selective detection of dopamine in sweat.
Patent Information
- Application Number
- CN202511273389.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-08
- Publication Date
- 2025-11-21
AI Technical Summary
In existing dopamine detection technologies, traditional sensor materials have poor conductivity, low electron transmission efficiency, weak anti-interference ability, and lack signal complementarity verification, resulting in insufficient detection sensitivity and limited accuracy.
By combining carbon quantum dot-doped carbon nanotubes with metal-organic frameworks (MOFs) for electrochemical and fluorescence sensors, the high sensitivity of electrochemical detection and the high selectivity of fluorescence detection can be used to achieve accurate detection of dopamine in sweat.
It improves the sensitivity and accuracy of sweat dopamine detection, overcomes the shortcomings of traditional sensors, and achieves highly sensitive and selective detection of dopamine.
Smart Images
Figure FT_1 
Figure FT_2 
Figure FT_3
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of biosensors, in particular to a carbon nanotube bridged functionalized mesoporous metal organic framework (MOFs) dual-mode sensor device and application of the device in rapid and high-sensitivity detection of dopamine in sweat. BACKGROUND
[0002] Dopamine is a catecholamine neurotransmitter that is essential for maintaining key functions of the immune system and central nervous system. As an important neurotransmitter in the human brain, dopamine facilitates essential communication between neurons and influences a wide range of physiological processes. Known as the "feel-good" neurotransmitter, dopamine is primarily released in response to positive stimuli, affecting mood and reward perception, and its dysfunction can lead to a variety of diseases. Abnormal dopamine levels are associated with a variety of nervous system diseases, so the detection of dopamine requires the construction of a high-sensitivity sensor.
[0003] In health assessment and personalized medical services, accurate detection of biomarkers such as dopamine in sweat is crucial for providing valuable physiological information. However, due to the low concentration of sweat biomarkers, individual differences in sweat production, and the need for efficient sweat collection, detecting sweat biomarkers presents challenges. In existing dopamine detection technologies, electrochemical sensors are widely studied due to their ease of operation and rapid response, but traditional sensors have the following shortcomings: poor conductivity of sensitive materials: although single MOFs materials have high specific surface area and active sites, the low electron transport efficiency leads to insufficient detection sensitivity; weak anti-interference ability: interference substances such as uric acid and ascorbic acid in sweat matrix easily compete for response sites, affecting detection accuracy; single function: most sensors rely on a single electrochemical signal, lack signal complementary verification, and detection reliability is limited. To address the above problems, the present application combines electrochemical sensors with fluorescent sensors, combining the high sensitivity of electrochemical detection with the high selectivity of fluorescent detection to achieve accurate detection of sweat dopamine. SUMMARY
[0004] The present application addresses the deficiencies of existing technology by researching carbon quantum dot doped carbon nanotube bridged metal organic framework and its preparation method, and exploring its application in sweat dopamine. The composite material has good electrochemical and fluorescent signal response to dopamine, and is a new type of composite material. The prepared sensor has excellent sweat dopamine detection performance.
[0005] The present application is realized by the following technical solutions: In a first aspect of the present application, a method for preparing carbon quantum dots is provided, comprising the following steps: A certain mass ratio of urea and citric acid is dissolved in deionized water, and hydrothermal synthesis is performed, followed by dialysis and freeze-drying to obtain carbon quantum dots.
[0006] Preferably, the mass ratio of urea and citric acid in the above step is 2:1.
[0007] In the second aspect of the present application, a preparation method of the carbon nanotube carboxylation and bridged metal organic framework is provided, comprising the following steps: A certain mass of carbon nanotubes is dispersed in concentrated HNO3, heated to reflux, centrifuged, and washed to obtain carboxylated carbon nanotubes.
[0008] A certain molar ratio of zinc acetate and dimethyl imidazole is dissolved in methanol respectively, stirred, mixed, and then carboxylated carbon nanotubes are added and left to stand to obtain carbon nanotube bridged metal organic framework.
[0009] In the third aspect of the present application, the above composite material is provided for use in dopamine detection. BRIEF DESCRIPTION OF DRAWINGS
[0010] Figure 1 Fourier infrared spectrum of carbon quantum dot doped carbon nanotube bridged metal organic framework Figure 2 Particle size distribution graph of carbon quantum dot doped carbon nanotube bridged metal organic framework Figure 3 Electrochemical cyclic voltammogram of carbon quantum dot doped carbon nanotube bridged metal organic framework DETAILED DESCRIPTION
[0011] It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the present application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the present application belongs.
[0012] In order to enable those skilled in the art to more clearly understand the technical solutions of the present application, the technical solutions of the present application will be described in detail below in conjunction with specific embodiments. If the specific conditions of the experiments are not specified in the embodiments, the specific conditions are generally in accordance with the conventional conditions, or in accordance with the conditions recommended by the reagent company; the reagents, consumables, etc. used in the following examples, unless otherwise specified, can be obtained through commercial channels. EMBODIMENTS
[0013] Preparation of carbon quantum dot doped carbon nanotube bridged metal organic framework The chemical reaction process is shown below, and the specific reaction steps and reaction conditions are as follows: , Zn(OAc)2·2H2O (0.8779 g, 4 mmol) and 2-methylimidazole (6.568 g, 80 mmol) were dissolved in 40 mL of methanol, respectively, and magnetically stirred for 10 minutes, mixed at room temperature and 10 mg of carboxylated carbon nanotubes were added and left for 24 h, centrifuged at 10000 rpm for 10 minutes, the white precipitate was collected, washed with methanol for 3 times, and dried at 60 ℃ under vacuum for 6 hours to obtain the ZIF-8@CNT composite material.
[0014] 0.1200 g of ZIF8@CNTs composite material, 0.0600 g of carbon quantum dots were dissolved in 30 mL of ethanol and 0.2 mL of Nafion solution was added, and a ZIF8@CNTs@CQDs dispersion was obtained after ultrasonic dispersion for 15 min, and an infrared spectrum was tested Figure 1 ) Example
[0015] The composite material obtained in Example 1 was applied in the field of dopamine detection.
[0016] The following examples will illustrate the application process of the composite material provided by the present application, but the present application is not limited to the examples.
[0017] (1) Infrared spectrum The composite material obtained in Example 1 was dried, the sample was mixed with KBr, ground uniformly, and pressed into a tablet, the tablet containing the composite material was placed in a Fourier infrared spectrometer, the test parameters were set, and the absorption trend of the composite material at different wave velocities was recorded, and the infrared spectrum of the composite material was obtained.
[0018] (2) Particle size The composite material obtained in Example 1 was dissolved in methanol, and the particle size and Zata potential analyzer were used to analyze the CQDs / ZIF-8@CNT composite material, and the particle size distribution curve was recorded, as shown in Figure 2 .
[0019] (3) Electrochemistry The composite material obtained in Example 1 was uniformly dropped on a glassy carbon electrode, and an infrared lamp was used to dry it, and the glassy carbon electrode coated with the composite material was placed in a three-electrode electrolytic cell, and the electrolytic cell contained a phosphate buffer solution containing uric acid and ascorbic acid, and the working electrode was the glassy carbon electrode coated with the composite material, the counter electrode was a platinum sheet, and the reference electrode was an Ag / AgCl electrode. Using constant potential method, the voltage applied to the working electrode was adjusted by the electrochemical workstation, and the cyclic voltammogram curve was recorded, and the cyclic voltammogram curve of the composite material was obtained, as shown in Figure 3 .
[0020] The above descriptions are only the preferred embodiments of the present application, and are not intended to limit the present application. The present application can have various modifications and changes for those skilled in the art. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.
Claims
1. A method for preparing CQDs / ZIF-8@CNT composite material, characterized in that, Includes the following steps: CQDs were prepared via a hydrothermal method using urea and citric acid. First, CNTs were functionalized by dissolving zinc acetate and 2-methylimidazole separately in methanol, then magnetically stirring and mixing at room temperature. The functionalized carbon nanotubes were then added, allowed to stand, centrifuged, washed, and vacuum dried to obtain the ZIF-8@CNT composite material. The ZIF-8@CNTs and CQDs solution were mixed, sonicated, and centrifuged. The glassy carbon electrode was then polished to a mirror finish using Al2O3 polishing powder, ultrasonically cleaned, and the composite material suspension was sprayed onto the surface of the glassy carbon electrode and irradiated with an infrared lamp.
2. A dual-mode sensor device based on carbon nanotube-bridged functionalized mesoporous MOFs, characterized in that, The device is prepared using the method described in any one of claims 1, which is based on a carbon nanotube-bridged functionalized mesoporous MOF dual-mode sensor, including CQDs synthesis and CNT functionalization.
3. An application of a dual-mode sensor device based on carbon nanotube-bridged functionalized mesoporous MOFs, characterized in that, The electrochemical and fluorescence dual-mode sensor based on the modified electrode of CQDs / ZIF-8@CNT composite material as described in claims 1-2 is applied in the field of smart wearables.