Microfluidic monitoring chip for environmental water body and monitoring method

Abstract

The invention discloses a microfluidic monitoring chip and a monitoring method for environmental water, and belongs to the technical field of water quality monitoring, the monitoring chip comprises a sealing layer, a flow path channel layer, a sensing detection layer and a communication layer, the sealing layer is connected with the flow path channel layer, the flow path channel layer is connected with the sensing detection layer, and the communication layer is connected with the sensing detection layer. The sensing detection layer is connected with the communication layer, and the sensing detection layer is used for detecting multiple parameters of a water body; the sealing layer is provided with a micro valve, the micro valve is provided with a first sealing element and a second sealing element, the first sealing element is a flexible sealing element, and the second sealing element is connected to the periphery of the first sealing element in a surrounding mode; the second sealing element is connected with a pressure monitoring unit and is used for monitoring the pressure of the micro valve; and the pressure monitoring unit is electrically connected with the communication layer. The anti-interference capacity is improved through the double sealing piece structures, monitoring is conducted in cooperation with the pressure monitoring unit, and leakage prevention is achieved through combination of hardware and software.

Description

Technical Field

[0001] This invention relates to the field of water quality monitoring technology, and more specifically, to a microfluidic monitoring chip and monitoring method for environmental water bodies. Background Technology

[0002] Microfluidic technology for water quality testing is an integrated analytical technique based on micron-scale fluid manipulation. By integrating laboratory functions (such as sampling, pretreatment, reaction, and detection) onto a small chip, it enables rapid, accurate, and portable monitoring of water quality parameters. The microchips manufactured using microfluidic technology are made from extremely low-cost materials, and trace amounts of reaction reagents can be pre-encapsulated, resulting in no secondary pollution to the environment from the materials or the trace reagents.

[0003] In recent years, with the rapid development of microfluidic technology, combining microfluidic technology with optical and electrical detection technology can achieve multi-index detection of water samples with little or no reagents. For example, Chinese patent document CN115932201A discloses a water quality detection device, method, and system based on a microfluidic chip. This water quality detection device based on a microfluidic chip includes a control module, a drive module, a microfluidic chip, and a detection module. The control module is communicatively connected to the drive module, the microfluidic chip, and the detection module. The drive module is connected to the microfluidic chip, and the microfluidic chip is connected to the detection module. The microfluidic chip integrates a valve unit for controlling the liquid flow path. The control module controls the drive module to execute suction and discharge commands, and controls the detection module to execute detection commands. The drive module is used to suction the water sample to be tested and the detection reagents through the microfluidic chip into the detection module, and to discharge the waste liquid generated by the detection module through the microfluidic chip. The detection module is used to digest the water sample to be tested using the detection reagents, and to detect the digested water sample.

[0004] However, some existing microfluidic monitoring chips have poor anti-interference capabilities when operating in field environments, and the flow path valve unit may leak. Summary of the Invention

[0005] The purpose of this invention is to provide a microfluidic monitoring chip for environmental water bodies, which solves the problems of poor anti-interference ability and potential leakage of flow path valve units in some existing microfluidic monitoring chips when operating in the field.

[0006] To achieve the above objectives, the present invention provides a microfluidic monitoring chip for environmental water bodies, comprising a sealing layer, a flow path layer, a sensing and detection layer, and a communication layer. The sealing layer is connected to the flow path layer, the flow path layer is connected to the sensing and detection layer, and the sensing and detection layer is connected to the communication layer. The sensing and detection layer is used to detect multiple parameters of the water body. The sealing layer is provided with a microvalve, which has a first sealing element and a second sealing element. The first sealing element is a flexible sealing element, and the second sealing element is connected to the outer periphery of the first sealing element. The second sealing element is connected to a pressure monitoring unit for monitoring the pressure of the microvalve. The pressure monitoring unit is electrically connected to the communication layer.

[0007] Furthermore, the second seal and the first seal are arranged in a stepped manner, with the top surface of the second seal being higher than the top surface of the first seal.

[0008] Furthermore, the thickness of the first seal is 190μm to 210μm, and the Shore hardness is 15A to 25A; the thickness of the second seal is 140μm to 160μm.

[0009] Furthermore, the pressure monitoring unit is provided in four groups, which are distributed at four points around the second seal.

[0010] Furthermore, the flow path layer is provided with independent flow paths for conventional pollution parameters, ammonia nitrogen isothermal flow path, and COD digestion flow path.

[0011] Furthermore, the sensing and detection layer integrates multiple detection units, including a conventional pollution parameter water quality detection unit, an ammonia nitrogen ion detection unit, a COD digestion detection unit, and an emerging pollutant identification module; the detection units are used to detect the water parameters of the corresponding flow path in the flow path channel layer.

[0012] Furthermore, the flow path layer is provided with a heating layer, which includes a first temperature zone and a second temperature zone, wherein the temperature of the first temperature zone is higher than the temperature of the second temperature zone; the first temperature zone corresponds to the COD digestion flow path, and the second temperature zone corresponds to the ammonia nitrogen isothermal flow path.

[0013] Furthermore, the flow path layer is provided with a heat insulation layer, which separates the heating layer from the sealing layer.

[0014] Furthermore, the flow path layer is provided with a temperature control layer, which is electrically connected to the heating layer.

[0015] This invention also provides a microfluidic monitoring method for environmental water bodies, employing a microfluidic detection chip, the method comprising:

[0016] High-turbidity water is filtered using micro-valve and filtration module, and the pressure of the micro-valve is monitored by a pressure monitoring unit.

[0017] Zoned temperature control is used to ensure the accuracy of low-temperature environment testing;

[0018] Simultaneously outputs data on conventional pollution parameters, ammonia nitrogen, COD, and emerging pollutants;

[0019] The test results are transmitted to the environmental protection supervision cloud platform in real time.

[0020] Compared with existing known technologies, the technical solution provided by this invention has the following beneficial effects:

[0021] This invention discloses a microfluidic monitoring chip for environmental water bodies. Through a dual-seal structure and the flexible material properties of the first seal, it can adapt to extreme external environments, improving the sealing performance and leak-proof capability of the micro-valve. A pressure monitoring unit monitors the pressure at the micro-valve in real time and feeds the data back to the communication layer, enabling personnel to promptly detect leaks. This combination of hardware and software achieves "zero leakage" for the micro-valve. The sealing layer further enhances the overall chip's sealing performance, ensuring stable operation even in heavy rain.

[0022] It is obvious that the elements or features described in the above individual embodiments can be used alone or in combination in other embodiments. Attached Figure Description

[0023] The dimensions and scales in the accompanying drawings do not represent the actual dimensions and scales of the product. The drawings are for illustrative purposes only, and some non-essential elements or features have been omitted for clarity.

[0024] Figure 1 This is a schematic diagram of the internal structure of the micro-valve in an embodiment of the present invention;

[0025] Figure 2 This is a schematic diagram (I) of the flow path layer, heating layer and heat insulation layer in an embodiment of the present invention;

[0026] Figure 3 This is a schematic diagram (II) of the structure of the flow path layer, heating layer and heat insulation layer in an embodiment of the present invention.

[0027] Explanation of reference numerals in the attached figures

[0028] 100. Housing; 110. First seal; 120. Second seal; 130. Pressure monitoring unit; 140. Piezoelectric actuator;

[0029] 210. Transparent detection layer;

[0030] 220. Flow path channel layer; 221. Flow path for conventional pollution parameters; 222. Ammonia nitrogen isothermal flow path; 223. COD digestion flow path;

[0031] 230. Heating layer; 240. Insulation layer; 250. UV-LED. Detailed Implementation

[0032] The present invention will now be described in detail with reference to the accompanying drawings. The embodiments described herein are merely preferred embodiments of the invention; those skilled in the art can conceive of other ways to implement the invention based on these preferred embodiments, and such other ways also fall within the scope of the invention.

[0033] Reference Figures 1-3 This embodiment provides a microfluidic monitoring chip for environmental water bodies, including a transparent detection layer 210, a sealing layer, a flow path layer 220, a sensing layer, and a communication layer. The transparent detection layer 210, made of 0.5mm thick borosilicate glass, is located on the outermost part of the chip and serves both observation and protection purposes. The sealing layer is connected to the flow path layer 220, increasing the overall chip's sealing performance and ensuring stable operation even in heavy rain. The flow path layer 220 is connected to the sensing layer, which in turn is connected to the communication layer. The sensing layer detects multiple parameters of the water body, outputs these parameters, and transmits them to the communication layer. The communication layer then transmits these parameters in real-time to an environmental monitoring cloud platform. The communication layer includes a transmission module supporting the LoRaWAN protocol and a wide-temperature-range solar power supply unit, which powers all units within the chip. The communication layer is located at the bottom of the chip.

[0034] The sealing layer incorporates a micro-valve, which has a first sealing element 110 and a second sealing element 120. The first sealing element 110 is a flexible sealing element, and the second sealing element 120 surrounds and connects to the outer periphery of the first sealing element 110. The first sealing element 110 is made of flexible material, which can conform to the valve seat surface, compensate for microscopic unevenness, and achieve initial sealing. The second sealing element 120 has relatively higher rigidity, forming an "outer protective wall" to block external impacts and particulate matter intrusion, and bears the main pressure load. Through the dual sealing structure and the flexible material characteristics of the first sealing element 110, it can adapt to extreme external environments, improving the sealing performance and leakage prevention capability of the micro-valve. The second sealing element 120 is connected to a pressure monitoring unit 130 for monitoring the pressure of the micro-valve. The pressure monitoring unit 130 is electrically connected to the communication layer. By monitoring the pressure at the micro-valve in real time through the pressure monitoring unit 130 and feeding the data back to the communication layer, the operator can promptly detect micro-valve leakage, thus achieving "zero leakage" of the micro-valve through a combination of hardware and software.

[0035] It should be noted that "zero leakage" here means achieving near 100% leak prevention. The pressure monitoring unit 130 uses a pressure sensor with a sensitivity of 0.01 kPa, which can immediately transmit a signal to the alarm device to trigger an alarm when a leak occurs. In some embodiments, the pressure monitoring unit 130, i.e., the pressure sensor, is provided with four sets, distributed at four points around the second seal 120. This four-point pressure monitoring provides a wider coverage and more accurate monitoring data.

[0036] Furthermore, the micro-valve's structure includes a titanium alloy housing 100, with a coefficient of thermal expansion of 8.6 × 10⁻⁶. -6 The temperature range of 100℃ ensures stable operation of the micro-valve within a temperature range of -40℃ to 150℃. Both the first seal 110 and the second seal 120 are located inside the housing 100. The second seal 120 and the first seal 110 are arranged in a stepped configuration, with the top surface of the second seal 120 higher than the top surface of the first seal 110. This physical height difference creates a barrier, requiring the medium to pass through two lines of defense sequentially, significantly extending the leakage path. If the medium breaches the first seal 110, it must travel to the higher plane of the second seal 120 to leak. This process generates a throttling effect due to the abrupt change in flow path, reducing the leakage rate and flow rate. The first seal 110 is made of PDMS material with a thickness of 190μm to 210μm, preferably 200μm; its Shore hardness is 15A to 25A, preferably 20A. The material and structure of the first seal 110 result in a compression rebound rate >95% at -20℃, enabling its application in extremely cold waters. The second seal 120 is made of fluororubber, which acts as a reinforcing ring and has the advantages of acid and alkali resistance. Its thickness is 140μm to 160μm, preferably 150μm, which can be used in industrial wastewater scenarios.

[0037] The microvalve also incorporates an ultrasonic cleaning and filtration module, primarily employing a piezoelectric actuator 140. This actuator 140 contacts the pressure monitoring unit 130, enabling precise fluid control through high-precision, rapid-response mechanical deformation. The ultrasonic cleaning and filtration module can handle high-turbidity water environments, maintaining the microvalve's patency even in water with a turbidity of 200 NTU. The piezoelectric actuator 140 has a response time of <5ms, effectively resisting water flow impact and further enhancing the chip's anti-interference capabilities.

[0038] Specifically, water samples enter the flow channel layer 220, where the sensing and detection layer detects various pollution parameters. The flow channel layer 220 is equipped with three independent flow channels: a conventional pollution parameter flow channel 221, an ammonia nitrogen isothermal flow channel 222, and a COD digestion flow channel 223. These three flow channels are independent and made of PDMS material. The sensing and detection layer integrates multiple detection units, including a conventional pollution parameter water quality detection unit, an ammonia nitrogen ion detection unit, a COD digestion detection unit, and an emerging pollutant identification module. These detection units are used to detect water parameters in the corresponding flow channels of the flow channel layer. The conventional pollution parameter flow channel 221 is mainly used in conjunction with the conventional pollution parameter water quality detection unit to detect the five conventional parameters of the water: pH, dissolved oxygen (DO), turbidity, conductivity, and temperature. The ammonia nitrogen isothermal flow channel 222 is mainly used in conjunction with the ammonia nitrogen ion detection unit to detect the ammonia nitrogen ion parameter in the water. COD digestion flow path 223 mainly works with the COD digestion detection unit to detect COD values. In addition, it also works with the emerging pollutant identification module to detect microplastics and PFAS. Specific water environment detection parameters are shown in Table 1 below.

[0039] Table 1. Water Environment Monitoring Parameters

[0040]

[0041]

[0042] Therefore, compared with traditional detection technologies, the single chip of this invention enables simultaneous detection of 9 parameters, covering the basic items of the GB 3838-2002 surface water standard. Moreover, compared with traditional equipment (weighing 10kg), the chip of this invention weighs only 150g, a significant reduction in weight, and correspondingly, a significant reduction in size.

[0043] It should be noted that the microplastic detection unit uses a gold nanocone SERS substrate processed by femtosecond laser with a cone spacing of 100-200 nm. The PFAS detection unit uses a CRISPR-Cas12a biosensor combined with a magnetic enrichment column (Fe3O4@ZIF-8).

[0044] Furthermore, traditional methods typically take 3-7 days from sampling and delivery to receiving the test report, while this invention takes less than 5 minutes from in-situ testing to cloud transmission, representing a speed improvement of approximately 1000 times. Traditional methods cover 3-5 water body detection parameters, while this invention covers 9+, an improvement of approximately 2 times. The labor cost for sampling and testing using traditional methods is approximately 200 yuan per sample, while the chip cost of this invention is 60 yuan per chip, reducing costs by 90%.

[0045] Understandably, both the ammonia-nitrogen isothermal flow path 222 and the COD digestion flow path 223 require heating devices. A heating layer is provided in the flow path channel layer 220, and the heating layer uses partitioned pipes for zoned heating. The heating layer includes a first temperature zone and a second temperature zone, with the temperature of the first temperature zone being higher than that of the second temperature zone. The temperature range of the first temperature zone is 80–120℃, and the temperature range of the second temperature zone is 37±0.5℃. The first temperature zone corresponds to the COD digestion flow path 223, and the second temperature zone corresponds to the ammonia-nitrogen isothermal flow path 222. The first temperature zone is equipped with a dense spiral heating wire and a purple UV-LED 250. The spiral heating wire is specifically a double-spiral Pt resistor (R = 20Ω), with a temperature control accuracy of ±0.5℃, a thermal response time ≤15 seconds, and a power consumption of 1.8W. The second temperature zone is equipped with a sparse serpentine heating wire, specifically a serpentine Pt resistor (R = 50Ω), with a temperature control accuracy of ±0.3℃, a thermal response time ≤10 seconds, and a power consumption of 0.3W. Temperature sensors are installed in both the ammonia nitrogen isothermal flow path 222 and the COD digestion flow path 223 to detect the temperature.

[0046] To avoid temperature interference, a heat insulation layer 240 is provided in the flow path layer 220, which separates the heating layer 230 from the sealing layer. The heat insulation layer 240 adopts a microporous aerogel structure, is 1 mm thick, and has a thermal conductivity of 0.02 W / m·K. A temperature control layer is provided in the flow path layer 220, which is electrically connected to the heating layer 230. An FPGA and a temperature PID controller are installed in the temperature control layer. The PID controller uses a PID algorithm to regulate the temperature in real time based on the temperature signal detected by the temperature sensor.

[0047] A microfluidic monitoring method for environmental water bodies, employing a microfluidic detection chip, includes the following steps:

[0048] High-turbidity water is filtered using micro-valve and filter module, and the pressure of the micro-valve is monitored by pressure monitoring unit 130.

[0049] Zoned temperature control is used to ensure the accuracy of low-temperature environment testing;

[0050] Simultaneously outputs data on conventional pollution parameters, ammonia nitrogen, COD, and emerging pollutants;

[0051] The test results are transmitted to the environmental protection supervision cloud platform in real time.

[0052] Example 1

[0053] Test scenario: Verification of river water pollution monitoring

[0054] Location: Mid-section of a waterway in a certain village

[0055] Environmental conditions: Temperature 16℃, Turbidity 85 NTU, Light rain.

[0056] Test results:

[0057] Table 2 Detection Results of Example 1

[0058] parameter Measured values Standard Limit Exceeding the standard by multiple pH 8.6 6-9 - ammonia nitrogen 12.3 mg / L 1.0 mg / L 12.3× COD 78mg / L 20mg / L 3.9× microplastics 260 particles / L No standard - PFOS 0.15ppb 0.05ppb 3.0×

[0059] Example 2

[0060] Test scenario: Real-time monitoring of black and odorous water bodies in rural areas

[0061] Location: Downstream river channel in a rural area

[0062] Pollution characteristics: high turbidity (120 NTU), hydrogen sulfide odor, dissolved oxygen ≤1 mg / L

[0063] Chip Applications:

[0064] Leak-proof valve: Fluororubber sealing ring resists hydrogen sulfide corrosion; piezoelectric actuator 140 quickly cuts off sewage with a solids content of 15%.

[0065] Thermal management: A constant-temperature flow path (37℃) ensures the sensitivity of the ammonia nitrogen electrode in low-temperature wastewater (8℃) for multi-parameter detection.

[0066] Dissolved oxygen 0.3 mg / L (triggers hypoxia alarm)

[0067] Ammonia nitrogen 8.7 mg / L (8 times the Class V standard for surface water)

[0068] Microplastics detected: fibrous PET (Raman peak 1720 cm⁻¹) -1 )

[0069] Treatment effect: Real-time data linkage activated the aeration equipment, and dissolved oxygen rose to 2.5 mg / L after 48 hours.

[0070] Table 3. Compatibility Analysis of Black and Odorous Water Body Monitoring

[0071] Technical pain points This chip solution High turbidity blockage 200NTU Ultrasonic Self-Cleaning + Stepped Leak-Proof Valve Sulfide corrosion Fluororubber sealing rings (pH 0-14 resistant) Oxygen deficiency caused sensor failure Clark electrode anti-sulfide coating Odor-causing substance identification Integrated hydrogen sulfide electrochemical sensor (optional)

[0072] Example 3

[0073] Test scenario: Early warning of blue-green algae bloom in a reservoir

[0074] Location: A drinking water source reservoir in a certain city (eutrophication risk area)

[0075] Demand: Early warning of algal toxins (Microcystis-LR)

[0076] Innovative applications of chips:

[0077] Partition flow path optimization:

[0078] COD digestion flow path integrates an algal toxin immunosensor (replacing UV-LED).

[0079] Magnetic enrichment column for concentrating trace algal toxins (detection limit 0.1 μg / L)

[0080] Synchronous monitoring parameters:

[0081] Chlorophyll a (fluorescence method): 25 μg / L → warning threshold pH 8.7 → indicator of algal activity

[0082] Microcystis-LR: 0.32 μg / L (exceeds WHO limit)

[0083] Response mechanism: Data triggers the reservoir management department to activate the ultrasonic algae removal device.

[0084] Example 4

[0085] Test Scenario: Water Quality Safety Monitoring and Early Warning System for Aquaculture Ponds Pollution Sources: Ammonia nitrogen / COD exceeding standards due to aquaculture feeding, antibiotic residues Monitoring Challenges: Water turbidity (algae + feed), temperature stratification, lack of power Risks: Summer cyanobacteria blooms causing fish mortality

[0086] Technical adaptation solution

[0087] Table 4. Technical Adaptation Scheme for Example 4

[0088]

[0089] Table 5 Key Monitoring Data (Summer High Temperature Period)

[0090]

[0091]

[0092] The above embodiments demonstrate that, through innovative leak-proof valve design (stepped, double-sealed structure), zoned heating flow path (37℃ constant temperature / 120℃ digestion), and multi-parameter sensor integration, in-situ simultaneous detection of five conventional pollution parameters of surface water, ammonia nitrogen, COD, and emerging pollutants can be achieved. The chip meets IP68 protection standards and -20℃ low-temperature start-up requirements, supports ultrasonic self-cleaning (200 NTU high-turbidity water), magnetic enrichment of trace pollutants (PFAS detection limit 0.01 ppb), and direct data transmission to the environmental protection platform (LoRaWAN protocol), solving the industry pain points of poor field adaptability and incomplete parameter coverage of traditional monitoring equipment. The chip operates continuously under heavy rain conditions, transmitting data in real time to the provincial environmental protection department platform, triggering the closure of downstream water intakes and preventing major drinking water safety incidents.

[0093] In the description of this invention, it should be noted that the terms "front," "rear," "left," "right," "upper," "lower," "top," "bottom," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing the invention and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0094] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0095] The scope of protection of this invention is defined only by the claims. Thanks to the teachings of this invention, those skilled in the art will readily recognize that alternative structures to the structures disclosed herein can be used as feasible alternative implementations, and that the implementations disclosed herein can be combined to produce new implementations, which also fall within the scope of the appended claims.

Claims

1. A microfluidic monitoring chip for environmental water bodies, comprising a sealing layer, a flow path layer, a sensing and detection layer, and a communication layer, wherein the sealing layer is connected to the flow path layer, the flow path layer is connected to the sensing and detection layer, the sensing and detection layer is connected to the communication layer, and the sensing and detection layer is used to detect multiple parameters of the water body; Its features are, The sealing layer is provided with a micro valve, which has a first sealing element and a second sealing element. The first sealing element is a flexible sealing element, and the second sealing element is connected around the outer periphery of the first sealing element. The second sealing element is connected to a pressure monitoring unit for monitoring the pressure of the micro valve. The pressure monitoring unit is electrically connected to the communication layer.

2. The microfluidic monitoring chip for environmental water bodies according to claim 1, characterized in that, The second seal and the first seal are arranged in a stepped manner, with the top surface of the second seal being higher than the top surface of the first seal.

3. A microfluidic monitoring chip for environmental water bodies according to claim 2, characterized in that, The thickness of the first seal is 190μm to 210μm, and the Shore hardness is 15A to 25A; the thickness of the second seal is 140μm to 160μm.

4. A microfluidic monitoring chip for environmental water bodies according to claim 1, characterized in that, The pressure monitoring unit is provided in four groups, which are distributed at four points around the second seal.

5. A microfluidic monitoring chip for environmental water bodies according to claim 1, characterized in that, The flow path layer is equipped with independent flow paths for conventional pollution parameters, ammonia nitrogen isothermal flow path, and COD digestion flow path.

6. A microfluidic monitoring chip for environmental water bodies according to claim 5, characterized in that, The sensing and detection layer integrates multiple detection units, including a conventional pollution parameter water quality detection unit, an ammonia nitrogen ion detection unit, a COD digestion detection unit, and an emerging pollutant identification module; the detection units are used to detect the water parameters of the corresponding flow path in the flow path channel layer.

7. A microfluidic monitoring chip for environmental water bodies according to claim 5, characterized in that, The flow path layer is provided with a heating layer, which includes a first temperature zone and a second temperature zone. The temperature of the first temperature zone is higher than that of the second temperature zone. The first temperature zone corresponds to the COD digestion flow path, and the second temperature zone corresponds to the ammonia nitrogen isothermal flow path.

8. A microfluidic monitoring chip for environmental water bodies according to claim 7, characterized in that, The flow path layer is provided with a heat insulation layer, which separates the heating layer from the sealing layer.

9. A microfluidic monitoring chip for environmental water bodies according to claim 7, characterized in that, The flow path layer is provided with a temperature control layer, which is electrically connected to the heating layer.

10. A microfluidic monitoring method for environmental water bodies, employing a microfluidic detection chip, characterized in that, The method includes: High-turbidity water is filtered using micro-valve and filtration module, and the pressure of the micro-valve is monitored by a pressure monitoring unit. Zoned temperature control is used to ensure the accuracy of low-temperature environment testing; Simultaneously outputs data on conventional pollution parameters, ammonia nitrogen, COD, and emerging pollutants; The test results are transmitted to the environmental protection supervision cloud platform in real time.

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