Control system of multi-step droplet and water absorption controllable microfluidic chip

Abstract

The application provides a kind of multi-step droplet and water absorption controllable microfluidic chip control system, including rack, the rack is provided with main translation stage module, the main translation stage module carries and drives microfluidic chip moves along horizontal direction, the rack is correspondingly provided with droplet module for quantitative drop of sample addition area of microfluidic chip, detection module for microfluidic chip detection, needle water absorption module for pluggable fixed fluid suction piece of microfluidic chip to the top along the microfluidic chip movement path, and the droplet module is set in the initial end of the microfluidic chip movement path, the detection module is set in the middle of the microfluidic chip movement path, the needle water absorption module is set in the end of the microfluidic chip movement path. By delaying droplet and mechanical needle delaying water absorption paper and flow channel contact, the reaction time is accurately controlled, the immune response is ensured to proceed fully, and the accuracy and repeatability of detection are improved.

Description

Technical Field

[0001] This invention belongs to the field of medical testing equipment technology, and specifically relates to a control system for a microfluidic chip with controllable multi-step dripping and water absorption. Background Technology

[0002] Microfluidic chip technology has been widely used in the field of in vitro diagnostics due to its advantages such as low reagent consumption, fast analysis speed, and ease of integration and automation. Among them, microfluidic chips based on droplet and water absorption do not require an external pump source. They use the negative pressure generated by the water-absorbing material pre-installed inside the chip as the driving source. They have a simple structure and low cost, making them particularly suitable for point-of-care testing (POCT) scenarios.

[0003] However, existing droplet chips and their detection equipment still have some limitations. First, because the initiation of the siphon process is uncontrollable—usually starting as soon as the user adds the sample—the reaction time cannot be precisely controlled, potentially affecting the accuracy of the detection results due to incomplete reaction. Second, traditional detection equipment has limited functionality, typically only capable of performing end-stage optical detection, unable to actively intervene in the chip's flow control process, making it difficult to implement complex multi-step detection procedures.

[0004] CN117000323B discloses a microfluidic chip, including a substrate, a cover plate, and a microchannel formed by the substrate and the groove. The groove of the cover plate includes a channel region. Along its length, the channel region sequentially comprises a sample application region, a reaction region, and a control region. The sample application region, reaction region, and control region are fluidly connected to each other. A control valve is disposed within the control region, configured to control the flow of liquid from the reaction region to the control region. The control valve includes a movable fluid suction element and a braking orifice. The fluid suction element can contact or move away from the reaction region. The braking orifice is located on the side away from the reaction region and is a rectangular through-hole penetrating the substrate and the cover plate. A through-hole is disposed at the end of the fluid suction element away from the reaction region, and the through-hole is located within the braking orifice. Although the control region of this chip can control the flow of liquid within the channel region, manual operation of the control region is still required, causing inconvenience in use.

[0005] Therefore, there is an urgent need for an automated analytical instrument that can work in conjunction with microfluidic chips to precisely control the fluid processes involved in droplet and water absorption, thereby improving the accuracy, repeatability, and automation of detection. Summary of the Invention

[0006] The present invention aims to provide a control system for a microfluidic chip with controllable multi-step dripping and water absorption, and solves the problems existing in the prior art by optimizing the design of the detection instrument.

[0007] To achieve the above objectives, the technical solution of the present invention is as follows: a control system for a microfluidic chip with controllable multi-step dripping and water absorption includes a frame. A main translation stage module is mounted on the frame. The main translation stage module carries and drives the microfluidic chip to move horizontally. Above the microfluidic chip's movement path, the frame is equipped with a dripping module for quantitatively adding droplets to the sample application area of ​​the microfluidic chip, a detection module for detecting the microfluidic chip, and a needle water absorption module for pluggably fixing the fluid aspiration component of the microfluidic chip. The dripping module is located at the initial end of the microfluidic chip's movement path, the detection module is located in the middle of the microfluidic chip's movement path, and the needle water absorption module is located at the end of the microfluidic chip's movement path.

[0008] The frame is also equipped with a control module, which is electrically connected to the main translation stage module, the dripping module, the detection module, and the pin water absorption module. The control module controls the main translation stage module, the dripping module, the detection module, and the pin water absorption module to work together.

[0009] The frame includes a base plate, a left upright plate, and a right upright plate. The base plate is rectangular, and the left and right upright plates are fixed at both ends along the length of the base plate.

[0010] The main translation stage module includes a lead screw motor, a bracket, a guide rail, and a slider. The lead screw motor is fixed to the side of the base plate via a connecting plate. The bracket is fixed below the lead screw nut of the lead screw motor. The guide rail is fixed above the base plate, and the length direction of the guide rail is the same as the length direction of the base plate. The bracket moves along the length direction of the guide rail via a slider fixed at its bottom. The left and right ends of the bracket are provided with slots for inserting microfluidic chips.

[0011] The dripping module includes a first adapter plate, a fixed frame, a dripping bottle, a screw motor, a first eccentric wheel, a zero-point detection switch, and a droplet detector. One end of the first adapter plate is connected to the frame, and the other end is connected to the fixed frame. The dripping bottle is inserted into the fixed frame with its bottom drip opening extending downwards. The screw motor passes through the first adapter plate and is connected to the first eccentric wheel. The first eccentric wheel rotates and squeezes the dripping bottle. The zero-point detection switch and the droplet detector are fixed to the fixed frame. The upper end of the zero-point detection switch is connected to the shaft of the first eccentric wheel. The droplet detector is located at the lower end of the dripping bottle. The droplet detector detects the dripping volume of the dripping bottle and feeds it back to the control module. The control module controls the dripping volume of the dripping bottle through the zero-point detection switch.

[0012] The detection module is either a fluorescence detection component or a cell counting detection component.

[0013] The needle-absorbing module includes a first geared motor, a second eccentric wheel, a rotating pressure plate, a second adapter plate, and a needle. The geared motor is fixed to the frame via the second adapter plate. The output end of the geared motor is connected to the second eccentric wheel. The bottom of the needle passes through the second adapter plate. One end of the rotating pressure plate is sleeved on the outside of the second eccentric wheel and rotates with the second eccentric wheel. The other end rotates and contacts the upper surface of the needle, pressing down on the needle. The bottom of the needle passes through the second adapter plate and moves vertically downward under the pressure of the rotating pressure plate. The needle is positioned on the movement path directly above the through hole.

[0014] The main translation stage module is also provided with a lateral pressure module on its side. The lateral pressure module squeezes the microfluidic chip along the width direction of the base plate, so that the microfluidic chip is pressed tightly against the left vertical plate.

[0015] The lateral pressure module includes a top ball baffle, a second geared motor, a third eccentric wheel, and a ejector pin. The top ball baffle is fixed to the right side of the card tray, and both the card tray and the top ball baffle are provided with ejector pin holes through which the ejector pin can pass. One end of the ejector pin passes through the top ball baffle and enters the card tray. The second geared motor is fixed to the side of the card tray through the top ball baffle. The output end of the second geared motor is connected to the third eccentric wheel. After the third eccentric wheel rotates, it pushes the ejector pin to laterally press against the microfluidic chip. The lateral pressure module is electrically connected to the control module.

[0016] The initial end of the microfluidic chip's moving path is also equipped with a QR code recognition module, which reads the QR code information carried on the microfluidic chip and transmits the information to the control module.

[0017] The control system for a microfluidic chip with controllable multi-step dripping and water absorption obtained through the above technical solution has the following advantages:

[0018] 1. Controllable reaction and water absorption: By delaying the droplet flow and using mechanical needles to delay the contact between the absorbent paper and the flow channel, precise control of the reaction time is achieved, ensuring that the immune reaction proceeds fully and greatly improving the accuracy and repeatability of the test.

[0019] 2. High integration and automation: It integrates dripping, reaction, water absorption and detection into one device, which operates in a fully automatic manner, avoiding human operation errors and is particularly suitable for high-throughput and standardized clinical testing environments.

[0020] 3. Strong compatibility and flexibility: It can perform new detection processes that require precise fluid control, and can also skip the dripping and water absorption steps through program settings to directly perform end detection on traditional chips, making it widely applicable.

[0021] 4. Intelligent recognition: The system automatically identifies the test items and calls up the corresponding parameters through QR codes, realizing intelligent operation of "plug and test" and simplifying the user operation process. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the structure of the microfluidic chip described in this invention;

[0023] Figure 2 This is a schematic diagram of the control system of the microfluidic chip with controllable multi-step dripping and water absorption described in this invention;

[0024] Figure 3 This is a structural schematic diagram of the frame and main translation stage module described in this invention;

[0025] Figure 4 This is a schematic diagram of the structure of the card holder described in this invention;

[0026] Figure 5 This is a schematic diagram of the structure of the dripping module described in this invention;

[0027] Figure 6 This is a schematic diagram of the structure of the pin-insertion water absorption module described in this invention;

[0028] Figure 7 This is a schematic diagram of the lateral pressure module described in this invention.

[0029] In the diagram, 1. Frame; 2. Main translation stage module; 3. Dropping module; 4. Detection module; 5. Pin suction module; 6. Lateral pressure module; 7. QR code recognition module; 11. Base plate; 12. Left upright plate; 13. Right upright plate; 21. Screw motor; 22. Card holder; 22a. Card slot; 23. Guide rail; 24. Slider; 31. First adapter plate; 32. Fixing frame; 33. Dropping bottle; 33a. Dropping nozzle; 34. Screw motor; 35. First eccentric wheel; 36. Zero-point detection switch; 37. Droplet detector; 51, First geared motor; 52, Second eccentric wheel; 53, Rotating pressure plate; 54, Second adapter plate; 55, Insert pin; 61, Top bead baffle; 62, Second geared motor; 63, Third eccentric wheel; 64, Top pin; 64a, Top pin hole; 100, Microfluidic chip; 100a, Cover plate; 100b, Microchannel; 100c, Sample application area; 100d, Reaction area; 100e, Control area; 100f, Fluid aspiration element; 100f1, Through hole; 100g, Braking hole. Detailed Implementation

[0030] It should be noted that, unless otherwise specified, the embodiments and features described in the present invention can be combined with each other.

[0031] Unless otherwise defined, the technical terms used in the following embodiments have the same meanings as commonly understood by those skilled in the art. Unless otherwise specified, the experimental reagents used in the following embodiments are conventional biochemical reagents; and the experimental methods described are conventional methods.

[0032] This invention generally relates to the field of medical testing equipment technology, and to an automated analytical instrument that can work in conjunction with a microfluidic chip to precisely control the fluid process of droplets and water absorption, thereby improving the accuracy, repeatability and automation of testing.

[0033] The present invention will be further explained and described below with reference to the embodiments and accompanying drawings. It should be understood that the present invention is not limited to the specific embodiments described.

[0034] This invention relates to a control system for a multi-step droplet and water absorption controllable microfluidic chip based on the microfluidic chip structure described in CN117000323B, wherein the structure of CN117000323B is as follows:

[0035] like Figure 1 As shown, the microfluidic chip 100 includes a substrate, a cover plate 100a, and a microchannel 100b formed by the substrate and the groove. The groove of the cover plate includes a channel region. The channel region has a sample application area 100c, a reaction region 100d, and a control region 100e sequentially arranged along its length. The sample application area 100c, the reaction region 100d, and the control region 100e are fluidly connected to each other. A control valve is provided in the control region 100e, and the control valve is configured to control the liquid in the reaction region to flow into the microchannel 100b. The flow in the control zone is controlled by a control valve comprising a movable fluid suction element 100f and a braking hole 100g. The fluid suction element 100f can contact or move away from the reaction zone 100d. The braking hole 100g is located on the side away from the reaction zone 100d and is a rectangular through hole penetrating the substrate 100a and the cover plate 100b. A through hole 100f1 is provided at the end of the fluid suction element 100f away from the reaction zone, and the through hole 100f1 is located within the braking hole 100g.

[0036] We usually conduct the test manually. The main method is to insert a stick-shaped tool into the through hole of the fluid suction component 100f and manually pull it within the range of the brake hole 100g, so that the fluid suction component 100f contacts or moves away from the microchannel 100b.

[0037] like Figure 2As shown, this invention proposes a control system for a microfluidic chip with controllable multi-step dripping and water absorption, including a frame 1. A main translation stage module 2 is mounted on the frame 1. The main translation stage module 2 carries and drives the microfluidic chip 100 to move horizontally. Above the moving path of the microfluidic chip 100, the frame 1 is equipped with a dripping module 3 for quantitatively dripping droplets onto the sample application area 100c of the microfluidic chip 100, a detection module 4 for detecting the microfluidic chip, and a needle water absorption module 5 for pluggable fixation of the fluid aspiration component 100f of the microfluidic chip 100. The dripping module 3 is located at the initial end of the moving path of the microfluidic chip 100, the detection module 4 is located in the middle of the moving path of the microfluidic chip 100, and the needle water absorption module 5 is located at the end of the moving path of the microfluidic chip 100.

[0038] Initially, keep the fluid aspirator 100f of the microfluidic chip 100 detached from the microchannel 100b. Place the microfluidic chip 100 on the main translation stage module 2. Quantitatively add liquid to the sample application area 100c via the dripping module 3. The liquid enters the microchannel, flows, and reacts. After the reaction is complete, move the microfluidic chip towards the end of the path via the main translation stage module 2. Stop when the lower end of the needle aspiration module 5 is aligned with the through-hole 100f1 of the fluid aspirator 100f. At this point, the needle aspiration module 5 inserts into the through-hole 100f1 of the fluid aspirator 100f, thus aspirating the fluid aspirator 100f. 00f is fixed, and then the microfluidic chip 100 is moved a short distance to the end of the path by the main translation stage module 2. At this time, since the fluid suction component 100f inserted at the bottom of the pin water suction module 5 remains stationary, it moves relative to the microfluidic chip 100 towards the microchannel 100b and contacts the microchannel 100b. It can absorb excess water in the microchannel 100b. After absorption, the bottom of the pin water suction module 5 is separated from the fluid suction component 100f, and the reaction area 100d of the microfluidic chip 100 is moved below the detection module 4 by the main translation stage module 2 for detection.

[0039] The frame 1 is also equipped with a control module, which is electrically connected to the main translation stage module 2, the dripping module 3, the detection module 4, and the needle water absorption module 5. The control module controls the main translation stage module 2, the dripping module 3, the detection module 4, and the needle water absorption module 5 to work together.

[0040] like Figure 3 As shown, the frame 1 includes a base plate 11, a left upright plate 12, and a right upright plate 13. The base plate 11 is rectangular, and the left upright plate 12 and the right upright plate 13 are respectively fixed at both ends of the base plate 11 along its length.

[0041] like Figure 4As shown, the main translation stage module 2 includes a lead screw motor 21, a card holder 22, a guide rail 23, and a slider 24. The lead screw motor 21 is fixed to the side of the base plate 11 via a connecting plate. The card holder 22 is fixed below the lead screw nut of the lead screw motor 21. The guide rail 23 is fixed above the base plate 11, and the length direction of the guide rail 23 is the same as the length direction of the base plate 11. Driven by the lead screw motor 21, the card holder 22 moves along the length direction of the guide rail 23 via the slider 24 fixed at its bottom. The left and right ends of the card holder 22 are provided with slots 22a for inserting the microfluidic chip 100.

[0042] like Figure 5 As shown, the dripping module 3 includes a first adapter plate 31, a fixing frame 32, a dripping bottle 33, a screw motor 34, a first eccentric wheel 35, a zero-point detection switch 36, and a droplet detector 37. One end of the first adapter plate 31 is connected to the frame 1, and the other end is connected to the fixing frame 32. The dripping bottle 33 is inserted into the fixing frame 32 with its bottom drip nozzle 33a extending downwards. The screw motor 34 passes through the first adapter plate 31 and is connected to the first eccentric wheel 35. After the first eccentric wheel 35 rotates, it squeezes the dripping bottle 33. The zero-point detection switch 36 and the droplet detector 37 are fixed on the fixing frame 32 at their sides. The upper end of the 6 is connected to the shaft of the first eccentric wheel 35. The droplet detector 37 is located at the lower end of the dropper bottle 33. The droplet detector 37 detects the amount of liquid dripped from the dropper bottle 33 and feeds it back to the control module. One end of the droplet detector 37 emits light and the other end receives light. When a droplet passes through the middle, the light is blocked. When the blocked signal is received, it indicates that a droplet has fallen. The control module controls the amount of liquid dripped from the dropper bottle 33 through the zero-point detection switch 36. The control module controls the rotation angle or number of revolutions of the first eccentric wheel 35 by controlling the zero-point detection switch 36 to precisely control the number of drops in the dropper bottle 33, so as to realize the quantitative addition of droplets to the microfluidic chip 100.

[0043] The detection module 4 is either a fluorescence detection component or a cell counting detection component. Conventional methods use a fluorescence detection component for immunoassay; this component is existing technology and typically includes a fluorescence optical sensor, an excitation light source, and optical elements for filtering.

[0044] like Figure 6As shown, the pin-type water absorption module 5 includes a first reduction motor 51, a second eccentric wheel 52, a rotating pressure plate 53, a second adapter plate 54, and a pin 55. The first reduction motor 51 is fixed on the frame 1 through the second adapter plate 54. The output end of the first reduction motor 51 is connected to the second eccentric wheel 52. The bottom of the pin 55 passes through the second adapter plate 54. One end of the rotating pressure plate 53 is sleeved on the outside of the second eccentric wheel 52 and rotates through the second eccentric wheel 52. After the other end rotates, it contacts the upper surface of the pin 55 and presses down on the pin 55. The bottom of the pin 55 passes through the second adapter plate 54 and moves vertically downward under the pressure of the rotating pressure plate 53. The pin 55 is located on the moving path directly above the through hole 100f1. The pin-type water absorption module 5 is electrically connected to the control module. The control module controls the main translation stage module 2 to move the microfluidic chip 100, so that the through hole 100f1 of the fluid suction component 100f is directly below the pin 55, causing the pin 55 to be pressed down and inserted into the through hole 100f1 of the fluid suction component 100f. Due to the gravity of the pin 55, in order to prevent the pin 55 from disengaging from the through hole 100f1 when it is not subjected to the downward pressure of the second eccentric wheel 52, a return spring can be provided in the second adapter plate 54 into which the lower end of the pin 55 is inserted, so that it springs upward when no force is applied.

[0045] The main translation stage module 2 is also provided with a lateral pressure module 6 on its side. The lateral pressure module 6 presses the microfluidic chip 100 along the width direction of the base plate 11, so that the microfluidic chip 100 is pressed tightly against the left vertical plate 12. The lateral pressure module 6 is used to laterally press the microfluidic chip 100 when the bottom of the pin 55 is inserted into the through hole 100f1, to prevent it from shifting when the fluid suction component 100f absorbs water.

[0046] like Figure 7 As shown, the lateral pressure module 6 includes a top ball baffle 61, a second reduction motor 62, a third eccentric wheel 63, and a pin 64. The top ball baffle 61 is fixed to the right side of the card holder 22, and both the card holder 22 and the top ball baffle 61 are provided with pin holes 64a through which the pin 64 can pass. One end of the pin 64 passes through the top ball baffle 61 and enters the card holder 22. The second reduction motor 62 is fixed to the side of the card holder 22 through the top ball baffle 61. The output end of the high-speed motor 62 is connected to the third eccentric wheel 63. After the third eccentric wheel 63 rotates, it pushes the ejector pin 64 to press against the microfluidic chip 100 laterally. The lateral pressure module 6 is electrically connected to the control module. The control module controls the lateral pressure module 6 to apply pressure to the side of the microfluidic chip 100 when pressing the fluid suction component 100f at the bottom of the needle suction module 5. A reset spring can be installed in the ejector pin hole 64a at the side of the ejector pin 64 so that it rebounds and resets when no force is applied.

[0047] The initial end of the movement path of the microfluidic chip 100 is also provided with a QR code recognition module 7. The QR code recognition module 7 reads the QR code information carried on the microfluidic chip 100 and transmits the information to the control module. The control module is configured to call a pre-stored detection program corresponding to the QR code information. The detection program includes parameters such as the number of droplets, reaction time, and water absorption trigger timing.

[0048] Example

[0049] Taking immunofluorescence detection using the control system of this invention as an example, the specific workflow is as follows:

[0050] Insert the microfluidic chip 100 into the slot of the main translation stage module 2, and then start the control module. The main translation stage module 2 moves the microfluidic chip 100 to the QR code recognition module 7 to scan the code and obtain the detection item information (such as "Item A").

[0051] According to the preset program of "Project A", the main translation stage module 2 moves the microfluidic chip 100 to below the dripping module 3, and the control module 6 controls the motor of the dripping module 3 to rotate a predetermined number of times, adding 3 drops of trigger buffer solution.

[0052] After the dripping is completed, the main translation stage module 2 moves the microfluidic chip 100 to an idle position and starts timing, waiting for a reaction time of 180 seconds.

[0053] After the reaction time ends, the main translation stage module 2 moves the microfluidic chip 100 to the position of the pin-absorbing module 5. The control module 6 first controls the motor of the pin 55 to insert the pin 55 downwards into the absorbent paper (through hole 100f1 of the fluid suction component 100f) at the end of the chip. Subsequently, the second reduction motor 62 of the lateral pressure module 6 is started, and the third eccentric wheel 63 rotates to press and fix the microfluidic chip 100.

[0054] The main translation stage module 2 continues to move forward a short distance (e.g., 5mm). Since the pin 55 has been inserted into the absorbent paper and is in a fixed position, the movement of the main translation stage module 2 causes the microfluidic chip 100 to move relative to the pin 55, resulting in wrinkles or displacement of the absorbent paper, which then comes into contact with the microchannel inlet inside the chip, and the siphon process begins.

[0055] After water absorption is completed (the time can be set according to the program), the insertion pin 55 is lifted and retracted, the lateral pressure module 6 is released, and the main translation stage module 2 transports the microfluidic chip 100 to the bottom of the detection module 4 for scanning, detecting the fluorescence values ​​of the C line and T line within the reaction zone 100d.

[0056] The control module calculates the T / C ratio and obtains the concentration value according to the built-in algorithm, and finally outputs the detection results on the display screen.

[0057] It should be noted that, unless otherwise specified, the embodiments and features described in the present invention can be combined with each other.

[0058] In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, are only for the convenience of describing the invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the invention. Furthermore, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined with "first," "second," etc., may explicitly or implicitly include one or more of that feature. In the description of this invention, unless otherwise stated, "a plurality of" means two or more.

[0059] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "setting" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal communication between two components. Those skilled in the art will understand the specific meaning of the above terms in this invention based on the specific circumstances.

Claims

1. A control system for a microfluidic chip with controllable multi-step dripping and water absorption, comprising a frame (1), wherein a main translation stage module (2) is disposed on the frame (1), the main translation stage module (2) carrying and driving the microfluidic chip (100) to move horizontally, characterized in that, The frame (1) is provided with a dripping module (3) for quantitatively adding droplets to the sample application area (100c) of the microfluidic chip (100), a detection module (4) for detecting the microfluidic chip (100), and a needle suction module (5) for pluggably fixing the fluid aspiration component (100f) of the microfluidic chip (100). The dripping module (3) is located at the initial end of the movement path of the microfluidic chip (100), the detection module (4) is located in the middle of the movement path of the microfluidic chip (100), and the needle suction module (5) is located at the end of the movement path of the microfluidic chip (100). The frame (1) is also equipped with a control module. The control module is electrically connected to the main translation stage module (2), the dripping module (3), the detection module (4), and the needle water absorption module (5). The control module controls the main translation stage module (2), the dripping module (3), the detection module (4), and the needle water absorption module (5) to work together. The dripping module (3) includes a first adapter plate (31), a fixed frame (32), a dripping bottle (33), a screw motor (34), a first eccentric wheel (35), a zero-point detection switch (36), and a droplet detector (37). One end of the first adapter plate (31) is connected to the frame (1), and the other end is connected to the fixed frame (32). The dripping bottle (33) is inserted into the fixed frame (32), with the dripping nozzle (33a) at the bottom extending downwards. The screw motor (34) passes through the first adapter plate (31) and is connected to the first eccentric wheel (35). After the first eccentric wheel (35) rotates, it squeezes the dropper bottle (33). The zero-point detection switch (36) and the droplet detector (37) are fixed on the side of the fixed frame (32). The upper end of the zero-point detection switch (36) is connected to the rotating shaft of the first eccentric wheel (35). The droplet detector (37) is set at the lower end of the dropper bottle (33). The droplet detector (37) detects the amount of liquid dripped from the dropper bottle (33) and feeds it back to the control module. The control module controls the amount of liquid dripped from the dropper bottle (33) through the zero-point detection switch (36). The needle-absorbing module (5) includes a first geared motor (51), a second eccentric wheel (52), a rotating pressure plate (53), a second adapter plate (54), and a needle (55). The first geared motor (51) is fixed on the frame (1) through the second adapter plate (54). The output end of the first geared motor (51) is connected to the second eccentric wheel (52). The bottom of the needle (55) passes through the second adapter plate (54). One end of the rotating pressure plate (53) is sleeved on the outside of the second eccentric wheel (52) and rotates through the second eccentric wheel (52). The other end rotates and contacts the upper surface of the needle (55) and presses down on the needle (55). The bottom of the needle (55) passes through the second adapter plate (54) and moves vertically downward through the pressure of the rotating pressure plate (53). The needle (55) is located on the moving path directly above the through hole (100f1).

2. The control system for the multi-step dripping and water absorption controllable microfluidic chip according to claim 1, characterized in that, The frame (1) includes a base plate (11), a left upright plate (12), and a right upright plate (13). The base plate (11) is rectangular, and the left upright plate (12) and the right upright plate (13) are respectively fixed at both ends of the base plate (11) along its length.

3. The control system for the multi-step dripping and water absorption controllable microfluidic chip according to claim 2, characterized in that, The main translation stage module (2) includes a lead screw motor (21), a card holder (22), a guide rail (23), and a slider (24). The lead screw motor (21) is fixed to the side of the base plate (11) by a connecting plate. The card holder (22) is fixed below the lead screw nut of the lead screw motor (21). The guide rail (23) is fixed above the base plate (11), and the length direction of the guide rail (23) is the same as the length direction of the base plate (11). The card holder (22) moves along the length direction of the guide rail (23) by the slider (24) fixed at its bottom. The left and right ends of the card holder (22) are provided with slots (22a) for inserting microfluidic chips (100).

4. The control system for the multi-step dripping and water absorption controllable microfluidic chip according to claim 1, characterized in that, The detection module (4) is either a fluorescence detection component or a cell counting detection component.

5. The control system for the multi-step dripping and water absorption controllable microfluidic chip according to claim 3, characterized in that, The main translation stage module (2) is also provided with a lateral pressure module (6) on its side. The lateral pressure module (6) squeezes the microfluidic chip (100) along the width direction of the base plate (11) so that the microfluidic chip (100) is pressed tightly against the left upright plate (12).

6. The control system for the multi-step dripping and water absorption controllable microfluidic chip according to claim 5, characterized in that, The lateral pressure module (6) includes a top ball baffle (61), a second geared motor (62), a third eccentric wheel (63), and a ejector pin (64). The top ball baffle (61) is fixed to the right side of the card holder (22), and both the card holder (22) and the top ball baffle (61) are provided with ejector pin holes (64a) through which the ejector pin (64) can pass. One end of the ejector pin (64) passes through the top ball baffle (61) and enters the card holder (22). The second geared motor (62) is fixed to the side of the card holder (22) through the top ball baffle (61). The output end of the second geared motor (62) is connected to the third eccentric wheel (63). After the third eccentric wheel (63) rotates, it pushes the ejector pin (64) to press it laterally against the microfluidic chip (100). The lateral pressure module (6) is electrically connected to the control module.

7. The control system for the multi-step dripping and water absorption controllable microfluidic chip according to claim 1, characterized in that, The initial end of the movement path of the microfluidic chip (100) is also provided with a QR code recognition module (7). The QR code recognition module (7) reads the QR code information carried on the microfluidic chip (100) and transmits the information to the control module.

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