Incubator without fan and air inlet control method of incubator without fan

Abstract

The invention belongs to the technical field of cell culture, particularly provides a fan-free incubator and an air inlet control method of the fan-free incubator, and aims to solve the problems that air inlet of an existing fan-free incubator is prone to overshoot of carbon dioxide and uneven concentration of carbon dioxide and oxygen. Therefore, the draught-fan-free incubator comprises an incubator body, an inner container located in the incubator body and an air inlet device arranged in the inner container, and the air inlet device is arranged in the mode that when target gas is introduced into the inner container, part of air in the inner container can be firstly extracted to be mixed with the introduced target gas; and then mixed gas of air and target gas is introduced into the inner container. According to the incubator without the fan, the concentration of the target gas in each area of the inner container can be kept uniform, the gas in each area can be uniformly changed, the concentration of carbon dioxide and oxygen in each area of the inner container can be kept uniform and stable without a fan, and the incubator is convenient to use.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of cell culture, and specifically provides a fan-free incubator and an air inlet control method of the fan-free incubator. BACKGROUND

[0002] The three-gas incubator is a device for culturing anaerobic cells in vitro, which is derived from the carbon dioxide incubator and has high control accuracy for carbon dioxide and oxygen. The existing incubator usually circulates through a fan to avoid overshooting of the recovery concentration of carbon dioxide. However, the fan will generate vibration and turbulence, which will affect the yield of cell culture. The fan-free incubator adopts conventional air inlet, which will cause uneven concentration of carbon dioxide and oxygen in different areas and the phenomenon of carbon dioxide overshooting.

[0003] Correspondingly, there is a need for a new technical solution to solve the above technical problems. SUMMARY

[0004] The application aims to solve the above technical problems, that is, to solve the problems of carbon dioxide overshooting and uneven concentration of carbon dioxide and oxygen in the existing fan-free incubator.

[0005] In a first aspect, the application provides a fan-free incubator, which comprises a cabinet, an inner container located in the cabinet, and an air inlet device arranged in the inner container. The air inlet device is arranged to first extract part of the air in the inner container and mix it with the target gas introduced into the inner container, and then introduce the mixed gas of air and target gas into the inner container, so that the target gas quickly diffuses to each area of the inner container, and the concentration of the target gas in each area of the inner container is kept uniform.

[0006] In the preferred technical solution of the above fan-free incubator, the air inlet device comprises a mixing shell, an impeller arranged in the mixing shell, and an air inlet pipeline system penetrating through the mixing shell. The mixing shell has a first side wall and a second side wall arranged oppositely and a ring side wall connecting the first side wall and the second side wall. The first side wall is mounted on the top wall of the inner container, the impeller is rotatably mounted on the top wall of the inner container, the second side wall is provided with an air inlet, and the ring side wall is uniformly provided with a plurality of mixed gas outlets. The outlet of the air outlet pipeline of the air inlet pipeline system is arranged on the circumferential outer side of the impeller and faces the impeller.

[0007] In the preferred embodiment of the above-mentioned fanless incubator, the mixing shell is cylindrical; and / or, the cross-sectional area of ​​the air inlet is 30% to 70% of the total area of ​​the second sidewall; and / or, the total cross-sectional area of ​​the plurality of mixed gas outlets is 50% to 80% of the total area of ​​the annular sidewall; and / or, the air intake pipeline system includes an air outlet pipeline, a first air intake pipeline, a second air intake pipeline, a carbon dioxide gas source, and a nitrogen gas source, wherein the two ends of the first air intake pipeline are respectively connected to the carbon dioxide gas source and the inlet of the air outlet pipeline, and the two ends of the second air intake pipeline are respectively connected to the nitrogen gas source and the inlet of the air outlet pipeline.

[0008] In the preferred embodiment of the above-mentioned fanless incubator, an inlet solenoid valve and an electromagnetic pressure sensor are provided on the outlet pipe. The electromagnetic pressure sensor is used to detect the pressure at the port of the inlet solenoid valve when it is closed. Alternatively, a first solenoid valve is provided on the first inlet pipe. Or, a first butterfly filter is provided on the first inlet pipe, located between the first solenoid valve and the carbon dioxide source. Or, a second solenoid valve is provided on the second inlet pipe. Or, a second butterfly filter is provided on the second inlet pipe, located between the second solenoid valve and the nitrogen source.

[0009] In the preferred embodiment of the above-mentioned fanless incubator, the fanless incubator further includes a positive pressure exhaust valve, through which the environment inside the inner chamber is selectively connected to the external environment.

[0010] In the preferred embodiment of the above-mentioned fanless incubator, the air intake device is installed on the top wall of the inner liner, and the positive pressure exhaust valve is located in the bottom area of ​​the side wall of the inner liner.

[0011] In the preferred embodiment of the above-mentioned fanless incubator, there are two positive pressure exhaust valves, which are respectively located on two opposite side walls of the inner liner.

[0012] With the above technical solution adopted, the fanless incubator of this application is equipped with an air intake device in the inner liner. When the target gas is introduced into the inner liner, the air intake device can first draw out a part of the air in the inner liner and mix it with the introduced target gas. Then, the mixed gas is introduced into the inner liner, thereby enabling the target gas to be quickly diffused to all areas of the inner liner, keeping the target gas concentration in all areas of the inner liner uniform, and making the gas in each area change uniformly. Without the need for a fan, the concentration of carbon dioxide and oxygen in all areas of the inner liner can be kept uniform and stable, which is convenient to use and helps to ensure the yield of cell culture.

[0013] Furthermore, the air intake device includes a mixing shell, an impeller, and an air intake pipeline system. When air is introduced into the inner liner through the air intake pipeline system, the impeller rotates, thereby creating a low-pressure zone within the mixing shell. Air from the inner liner is drawn into the mixing shell through the air inlet, where it mixes with the nitrogen and / or carbon dioxide supplied by the air intake pipeline system to form a mixed gas. This mixed gas is then discharged through the outer peripheral mixed gas outlet and mixed with the air outside the mixing shell. This configuration is simple in structure, easy to assemble and use, and can effectively drive the airflow within the inner liner, allowing carbon dioxide and / or nitrogen to quickly diffuse to all areas of the inner liner during air intake. This ensures that the concentrations of carbon dioxide and oxygen in all areas of the inner liner remain uniform and stable, preventing over-adjustment of carbon dioxide or oxygen in individual areas.

[0014] Furthermore, making the cross-sectional area of ​​the air inlet 30% to 70% of the total area of ​​the second sidewall helps the air inside the liner to enter the mixing shell smoothly, reduces the resistance to air entering the mixing shell, and makes it easier for air to mix with carbon dioxide and / or nitrogen.

[0015] Furthermore, making the total cross-sectional area of ​​the gas mixture outlet 50% to 80% of the total area of ​​the annular sidewall helps the gas mixture to be smoothly discharged from the mixing shell, reduces the resistance to gas mixture discharge, and facilitates use.

[0016] Furthermore, the air intake system includes an air outlet pipe, a first air intake pipe, a second air intake pipe, a carbon dioxide source, and a nitrogen source. The first air intake pipe is used to connect the air intake pipe to the carbon dioxide source, and the second air intake pipe is used to connect the air outlet pipe to the nitrogen source. Carbon dioxide and nitrogen can be introduced simultaneously through a single air outlet pipe, and by controlling the air intake, uniform gas distribution within the inner liner can be achieved.

[0017] Furthermore, an intake solenoid valve and an electromagnetic pressure sensor are installed on the exhaust pipe. The intake solenoid valve is used to control the opening and closing of the exhaust pipe, thereby selectively supplying air to the inner liner. The electromagnetic pressure sensor is used to detect the pressure value in the exhaust pipe, so that the gas output from the exhaust pipe is kept within a certain pressure range, which can drive the impeller to rotate smoothly and thus allow the gas to mix and diffuse smoothly.

[0018] In a second aspect, this application provides an air intake control method for a fanless incubator, the air intake control method comprising: acquiring a target oxygen concentration and an initial oxygen concentration inside the inner chamber; subtracting the target oxygen concentration from the initial oxygen concentration to obtain an oxygen concentration difference; acquiring a target carbon dioxide concentration and an initial carbon dioxide concentration inside the inner chamber; subtracting the target carbon dioxide concentration from the initial carbon dioxide concentration to obtain a carbon dioxide concentration difference; selectively executing a rapid air intake mode or a fine-tuning air intake mode based on the oxygen concentration difference and the carbon dioxide concentration difference; wherein the total air intake in the rapid air intake mode is greater than the total air intake in the fine-tuning air intake mode.

[0019] In the preferred embodiment of the above-mentioned air intake control method for a fanless incubator, the step of "selectively executing a rapid air intake mode or a fine-tuning air intake mode based on the oxygen concentration difference and the carbon dioxide concentration difference" specifically includes: determining whether the oxygen concentration difference is greater than a first preset difference; determining whether the absolute value of the carbon dioxide concentration difference is greater than the first preset difference; if the oxygen concentration difference is greater than the first preset difference or the absolute value of the carbon dioxide concentration difference is greater than the first preset difference, then executing the rapid air intake mode; if the oxygen concentration difference is not greater than the first preset difference and the carbon dioxide concentration difference is not greater than the first preset difference, then executing the fine-tuning air intake mode.

[0020] In the preferred embodiment of the above-mentioned air intake control method for a fanless incubator, the step of "executing the rapid air intake mode" specifically includes: determining the target carbon dioxide intake volume and the target nitrogen intake volume based on the target carbon dioxide concentration, the initial carbon dioxide concentration, the target oxygen concentration, the initial oxygen concentration, and the volume of the inner liner; comparing the target carbon dioxide intake volume with the target nitrogen intake volume; if the target carbon dioxide intake volume is greater than the target nitrogen intake volume, then executing the first air intake mode; if the target carbon dioxide intake volume is not greater than the target nitrogen intake volume, then executing the second air intake mode; wherein, the first air intake mode is the first stage only In the first stage, carbon dioxide is introduced, and in the second stage, carbon dioxide and nitrogen are introduced simultaneously; the second air intake mode is that only nitrogen is introduced in the first stage, and only carbon dioxide is introduced in the second stage; and / or, the step of "executing the fine-tuned air intake mode" specifically includes: acquiring the actual oxygen concentration and actual carbon dioxide concentration in the inner liner in real time; subtracting the target oxygen concentration from the actual oxygen concentration to obtain a real-time oxygen concentration difference; subtracting the target carbon dioxide concentration from the actual carbon dioxide concentration to obtain a real-time carbon dioxide concentration difference; selectively controlling the air intake device to introduce nitrogen and / or carbon dioxide into the inner liner according to the real-time oxygen concentration difference and / or the real-time carbon dioxide concentration difference.

[0021] In the preferred embodiment of the above-mentioned air intake control method for a fanless incubator, the step of "determining the target carbon dioxide intake volume and the target nitrogen intake volume based on the target carbon dioxide concentration, the initial carbon dioxide concentration, the target oxygen concentration, and the initial oxygen concentration" specifically includes: according to formula V CO2 =(c 1CO2 -c 2CO2 The target carbon dioxide intake volume is calculated as V × V, where V CO2 The target carbon dioxide intake volume, c 1CO2 It is the target carbon dioxide concentration, c 2CO2 V is the initial carbon dioxide concentration, and V is the volume of the inner liner; according to the formula V N2 ×c 2O2 =(c 2O2 -c 1O2 The target nitrogen intake volume is calculated as V × V, where V N2 The target nitrogen intake volume, c 1O2 It is the target oxygen concentration, c 2O2The initial oxygen concentration; and / or, the step of "executing the first intake mode" specifically includes: controlling the intake device to introduce carbon dioxide into the inner liner; detecting the concentration of carbon dioxide in the inner liner in real time and recording it as the actual carbon dioxide concentration; when the actual carbon dioxide concentration reaches the set carbon dioxide concentration, controlling the intake device to introduce carbon dioxide and nitrogen into the inner liner; wherein, the set carbon dioxide concentration is not higher than the target carbon dioxide concentration; detecting the oxygen concentration in the inner liner in real time and recording it as the actual oxygen concentration; when the actual oxygen concentration decreases to the target oxygen concentration, ending the first intake mode and executing the fine-tuning intake mode; and / or, The steps of “executing the second intake mode” specifically include: controlling the intake device to introduce nitrogen into the inner liner; real-time detection of the oxygen concentration in the inner liner, recording it as the actual oxygen concentration; determining whether the nitrogen introduction cutoff point has been reached based on the target carbon dioxide concentration, the target oxygen concentration, and the actual oxygen concentration, and when the nitrogen introduction cutoff point is reached, controlling the intake device to introduce carbon dioxide into the inner liner; real-time detection of the carbon dioxide concentration in the inner liner, recording it as the actual carbon dioxide concentration; when the actual carbon dioxide concentration rises to the target carbon dioxide concentration, ending the second intake mode and executing the fine-tuning intake mode; and / or, “according to the…” The step of "selectively controlling the air intake device to introduce nitrogen and / or carbon dioxide into the inner liner based on the real-time oxygen concentration difference and / or the real-time carbon dioxide concentration difference" specifically includes: comparing the real-time oxygen concentration difference with a second preset difference; if the real-time oxygen concentration difference is not less than the second preset difference, then controlling the air intake device to introduce nitrogen into the inner liner; if the real-time oxygen concentration difference is less than the second preset difference, then not controlling the air intake device to introduce nitrogen into the inner liner; and / or, "selectively controlling the air intake device to introduce nitrogen and / or carbon dioxide into the inner liner based on the real-time oxygen concentration difference and / or the real-time carbon dioxide concentration difference". The steps for "nitrogen and / or carbon dioxide" specifically include: comparing the absolute value of the real-time carbon dioxide concentration difference with a third preset difference; if the absolute value of the real-time carbon dioxide concentration difference is less than the third preset difference, then not controlling the air intake device to introduce carbon dioxide into the inner liner; if the absolute value of the real-time carbon dioxide concentration difference is not less than the third preset difference, then determining whether the real-time carbon dioxide concentration difference is greater than 0; if the real-time carbon dioxide concentration difference is greater than 0, then controlling the air intake device to introduce nitrogen into the inner liner; if the real-time carbon dioxide concentration difference is less than 0, then controlling the air intake device to introduce carbon dioxide into the inner liner.

[0022] In the preferred technical solution of the above-mentioned air intake control method for a fanless incubator, the step of "determining whether the nitrogen cutoff point has been reached based on the target carbon dioxide concentration, the target oxygen set concentration, and the actual oxygen concentration" specifically includes: determining (1-c 1CO2 )×c 2O2 -c 1O2 Whether it is less than 0.1%; if the judgment result is "yes", it is determined that the nitrogen gas supply cutoff point has been reached; if the judgment result is "no", it is determined that the nitrogen gas supply cutoff point has not been reached; and / or, the set carbon dioxide concentration is 0.1% lower than the target carbon dioxide concentration; and / or, the first preset difference is not less than 1%; and / or, the second preset difference is 0.1% to 0.2%; and / or, the third preset difference is 0.1% to 0.2%.

[0023] When adopting the above technical solution, the air intake control method of the fanless incubator of this application includes obtaining the target oxygen concentration, actual oxygen concentration, target carbon dioxide concentration, and actual carbon dioxide concentration inside the inner chamber; subtracting the target oxygen concentration from the actual oxygen concentration to obtain the oxygen concentration difference; subtracting the target carbon dioxide concentration from the actual carbon dioxide concentration to obtain the carbon dioxide concentration difference; and selectively executing a rapid air intake mode or a fine-tuning air intake mode based on the oxygen concentration difference and the carbon dioxide concentration difference. This setting method selects an air intake mode that matches the current inner chamber environment based on the oxygen concentration difference and the carbon dioxide concentration difference, so that the gas concentration inside the inner chamber can be quickly restored to the target set value, which is convenient for use.

[0024] Furthermore, it is determined whether the difference in oxygen concentration and the difference in carbon dioxide concentration are greater than a first preset difference. If the difference in oxygen concentration or the difference in carbon dioxide concentration are greater than the first preset difference, a rapid air intake mode is executed. If the difference in oxygen concentration and the difference in carbon dioxide concentration are not greater than the first preset difference, a fine-tuning air intake mode is executed. This judgment method is simple and accurate, thereby improving reaction efficiency and enabling the rapid execution of the corresponding air intake mode.

[0025] Furthermore, when executing the rapid air intake mode, the target carbon dioxide intake volume and the target nitrogen intake volume are first determined. When the target carbon dioxide intake volume is greater than the target nitrogen intake volume, the first air intake mode is executed, which introduces carbon dioxide first, followed by nitrogen and carbon dioxide. When the target carbon dioxide intake volume is not greater than the target nitrogen intake volume, the second air intake mode is executed, which introduces nitrogen first, followed by carbon dioxide. This avoids gas waste, saves costs, and allows the gas environment inside the liner to be converted to the required gas environment more quickly. Attached Figure Description

[0026] The preferred embodiments of this application are described below with reference to the accompanying drawings, in which:

[0027] Figure 1 This is a schematic diagram of the fanless incubator of this application;

[0028] Figure 2 This is a schematic diagram of the hybrid housing and impeller structure of this application, which shows the exhaust pipe;

[0029] Figure 3 yes Figure 2 A cross-sectional view along the AA direction;

[0030] Figure 4 This is a three-dimensional structural diagram of the hybrid shell of this application;

[0031] Figure 5 This is a schematic diagram of the intake piping system of this application;

[0032] Figure 6 This is a flowchart of the main steps of the air intake control method for the fanless incubator of this application;

[0033] Figure 7 This is a flowchart illustrating an implementation method of the air intake control method for the fanless incubator of this application;

[0034] Figure 8 This is a flowchart of the step "Executing rapid air intake mode" in the air intake control method of the fanless incubator of this application;

[0035] Figure 9 This is a flowchart of the step "Executing the first air intake mode" in the air intake control method of the fanless incubator of this application;

[0036] Figure 10 This is a flowchart of the step "Executing the second air intake mode" in the air intake control method of the fanless incubator of this application;

[0037] Figure 11 This is a flowchart of the step "Executing fine-tuning the air intake mode" in the air intake control method of the fanless incubator of this application.

[0038] List of reference signs:

[0039] 1. Box body;

[0040] 2. Inner liner;

[0041] 9. Intake device; 91. Mixing housing; 911. First side wall; 912. Second side wall; 9121. Air inlet; 913. Annular side wall; 9131. Mixed gas outlet; 92. Impeller; 93. Intake piping system; 931. Outlet piping; 9311. Intake solenoid valve; 9312. Electromagnetic pressure sensor; 932. First intake piping; 9321. First solenoid valve; 9322. First butterfly filter; 933. Second intake piping; 9331. Second solenoid valve; 9332. Second butterfly filter. Detailed Implementation

[0042] Preferred embodiments of this application will now be described with reference to the accompanying drawings. Those skilled in the art should understand that these embodiments are merely illustrative of the technical principles of this application and are not intended to limit the scope of protection of this application.

[0043] It should be noted that in the description of this application, terms such as "inner" and "outer" indicating direction or positional relationships are based on the direction or positional relationships shown in the accompanying drawings. This is merely for ease of description and does not indicate or imply that the device or element must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation of this application. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0044] Furthermore, it should be noted that, in the description of this application, unless otherwise expressly specified and limited, the terms "set" and "connection" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a direct connection or an indirect connection through other components. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0045] As mentioned in the background section, existing fanless incubators are prone to carbon dioxide overshoot and uneven concentrations of carbon dioxide and oxygen during air intake.

[0046] This application provides a fanless incubator and a method for controlling the air intake of the fanless incubator, which can be carried out quickly and evenly, preventing over-adjustment of carbon dioxide and oxygen in the incubator and making it more convenient to use.

[0047] In the first aspect, please provide a fanless incubator that can achieve rapid gas diffusion without a fan when air is introduced into the inner chamber, so as to make the gas environment in each area of ​​the inner chamber change uniformly.

[0048] Specifically, please refer to Figure 1 The fanless incubator of this application includes a box body 1, an inner liner 2 located inside the box body 1, and an air intake device 9 disposed inside the inner liner 2.

[0049] The air intake device 9 is configured to first extract a portion of the air inside the inner liner 2 and mix it with the target gas when the target gas is introduced into the inner liner 2, and then introduce the mixture of air and target gas into the inner liner 2 so that the target gas can be quickly diffused to all areas of the inner liner 2, thereby keeping the target gas concentration in all areas of the inner liner 2 uniform.

[0050] The fanless incubator of this application is equipped with an air intake device 9 inside the inner liner. When the target gas is introduced into the inner liner 2, the air intake device 9 can first extract a portion of the air inside the inner liner 2 and mix it with the introduced target gas. Then, the mixed gas is introduced into the inner liner 2, thereby enabling the target gas to quickly diffuse to all areas of the inner liner 2, keeping the target gas concentration in all areas of the inner liner 2 uniform, and ensuring uniform gas changes in all areas. It can maintain a uniform and stable concentration of carbon dioxide and oxygen in all areas of the inner liner without the need for a fan, which is convenient to use and helps to ensure the yield of cell culture.

[0051] Preferably, please also refer to Figure 1 and Figure 2 The air intake device 9 includes a mixing housing 91, an impeller 92 disposed in the mixing housing 91, and an air intake pipeline system 93 that penetrates the mixing housing 91.

[0052] The mixing housing 91 has a first sidewall 911 and a second sidewall 912 disposed opposite to each other, and an annular sidewall 913 connecting the first sidewall 911 and the second sidewall 912. The first sidewall 911 is mounted on the top wall of the inner liner 2, and the impeller 92 is rotatably mounted on the top wall of the inner liner 2. The second sidewall 912 is provided with an air inlet 9121, and the annular sidewall 913 is provided with a plurality of mixed gas outlets 9131 evenly distributed. The outlet of the air outlet pipe 931 of the air inlet pipe system 93 is disposed on the circumferential outer side of the impeller 92 and faces the impeller 92.

[0053] When the air intake device 9 is in operation, the air intake pipeline system 93 supplies air to the inner liner 2, and the outlet of its air outlet pipeline 931 blows the gas toward the impeller 92, causing the impeller 92 to rotate. During the rotation of the impeller 92, a negative pressure is formed in its central area, and a positive pressure is formed on the outer periphery of the impeller 92. This draws air from the inner liner 2 into the mixing shell 91 through the air inlet 9121. This air mixes with the gas in the mixing shell 91 and is then discharged from the mixed gas outlet 9131 to the area inside the inner liner 2 outside the mixing shell 91 (see [reference]). Figure 3 This allows the gas inside the inner liner 2 to flow, accelerating the diffusion rate of the introduced gas and ensuring a uniform and stable transition of the gas environment inside the inner liner 2 towards the target environment. At the same time, it prevents over-adjustment of carbon dioxide and oxygen inside the inner liner 2, making it convenient to control the gas concentration.

[0054] Preferably, please refer to Figure 4 The mixing housing 91 is cylindrical. Setting the mixing housing 91 to a cylindrical shape makes it easier to set a uniform mixed gas outlet 9131 and facilitates use.

[0055] Preferably, the cross-sectional area of ​​the air inlet 9121 is 30% to 70% of the total area of ​​the second sidewall 912.

[0056] Making the cross-sectional area of ​​the air inlet 9121 30% to 70% of the total area of ​​the second sidewall 912 helps the air in the inner liner 2 to smoothly enter the mixing shell 91, reduces the resistance of air entering the mixing shell 91, and makes it easier for air to mix with carbon dioxide and / or nitrogen.

[0057] The air inlet 9121 can be an air intake port, which is coaxially arranged with the mixing housing 91 (see [reference]). Figure 4 Alternatively, air inlet 9121 can be multiple air inlets.

[0058] Preferably, the total cross-sectional area of ​​the plurality of mixed gas outlets 9131 is 50% to 80% of the total area of ​​the annular sidewall 913.

[0059] Making the total cross-sectional area of ​​the gas mixture outlet 9131 50% to 80% of the total area of ​​the annular sidewall 913 helps the gas mixture to be smoothly discharged from the mixing housing 91, reduces the resistance to gas mixture discharge, and facilitates use.

[0060] Preferably, please refer to Figure 4 The mixed gas outlet 9131 includes multiple gas outlet holes, which are evenly distributed on the annular sidewall 913.

[0061] Preferably, please refer to Figure 5 The intake piping system 93 includes an exhaust piping 931, a first intake piping 932, a second intake piping 933, a carbon dioxide source (not shown in the figure), and a nitrogen source (not shown in the figure). The two ends of the first intake piping 932 are connected to the carbon dioxide source and the inlet of the exhaust piping 931, respectively. The two ends of the second intake piping 933 are connected to the nitrogen source and the inlet of the exhaust piping 931, respectively.

[0062] The intake pipe system 93 of the present invention has a first intake pipe 932 for connecting the intake pipe 931 to a carbon dioxide gas source, and a second intake pipe 933 for connecting the exhaust pipe 931 to a nitrogen gas source. The intake of carbon dioxide and nitrogen can be achieved simultaneously through an exhaust pipe 932, and the intake can be controlled to achieve uniform gas change in the inner liner.

[0063] Preferably, please continue reading. Figure 5An intake solenoid valve 9311 and an electromagnetic pressure sensor 9312 are installed on the exhaust pipe 931. The electromagnetic pressure sensor 9312 is used to detect the pressure at the port of the intake solenoid valve 9311 when the intake solenoid valve 9311 is closed.

[0064] An intake solenoid valve 9311 and an electromagnetic pressure sensor 9312 are installed on the exhaust pipe 931. The intake solenoid valve 9311 is used to control the opening and closing of the exhaust pipe 931, thereby selectively supplying air to the inner liner 2. The electromagnetic pressure sensor 9312 is used to detect the pressure value in the exhaust pipe 931, so that the gas output from the exhaust pipe 931 is kept within a certain pressure range, which can drive the impeller 92 to rotate smoothly, thereby enabling the gas to mix and diffuse smoothly.

[0065] Preferably, please continue reading. Figure 5 A first solenoid valve 9321 is installed on the first intake pipe 932. The first solenoid valve 9321 is used to control the opening and closing of the first intake pipe 932 so as to connect the carbon dioxide gas source when needed.

[0066] Preferably, please continue reading. Figure 5 A first butterfly filter 9322 is installed on the first air intake pipe 932, and the first butterfly filter 9322 is located between the first solenoid valve 9321 and the carbon dioxide gas source. The first butterfly filter 9322 is used to filter carbon dioxide to kill bacteria carried in it.

[0067] Preferably, please continue reading. Figure 5 A second solenoid valve 9331 is installed on the second air intake pipe 933. The second solenoid valve 9331 is used to control the opening and closing of the second air intake pipe 933 so as to connect the nitrogen gas source when needed.

[0068] Preferably, please continue reading. Figure 5 A second butterfly filter 9332 is installed on the second air intake pipe 933, and the second butterfly filter 9332 is located between the second solenoid valve 9331 and the nitrogen source. The second butterfly filter 9332 is used to filter the nitrogen to kill bacteria carried in it.

[0069] Preferably, the fanless incubator also includes a positive pressure exhaust valve, through which the environment inside the inner liner 2 is selectively connected to the external environment. By further configuring the positive pressure exhaust valve, when air is introduced into the inner liner 2, the pressure inside the inner liner 2 becomes sufficiently high, allowing the air inside the inner liner 2 to be discharged towards the external environment through the positive pressure exhaust valve. This allows the gas environment inside the inner liner 2 to quickly transform into the required gas environment. In addition, when the gas environment inside the inner liner 2 transforms into the required gas environment, the air intake pipeline system 93 is reduced or stopped, causing the pressure inside the inner liner 2 to decrease, thereby closing the positive pressure exhaust valve. This ensures that the environment inside the inner liner 2 remains stable. The inclusion of the positive pressure exhaust valve makes it more convenient to use.

[0070] Preferably, the air intake device 9 is installed on the top wall of the inner liner 2, and the positive pressure exhaust valve is located in the bottom area of ​​the side wall of the inner liner 2. This arrangement, with gas introduced at the top and the original gas discharged at the bottom, helps to prevent newly introduced gas from being accidentally discharged, and also helps to ensure that the gas flow is quickly and evenly mixed.

[0071] Preferably, there are two positive pressure exhaust valves, which are respectively installed on two opposite side walls of the inner liner 2.

[0072] In addition to the above structure, the fanless incubator of this application also includes a heating mechanism for heating the inner liner. The heating mechanism can be a segmented heating mechanism of an existing incubator. For example, the heating mechanism includes multiple heating elements, which heat different areas of the inner liner respectively, so that the temperature of each area of ​​the inner liner remains stable.

[0073] The fanless incubator of this application also includes a controller configured to execute the air intake control method of the fanless incubator.

[0074] In a second aspect, this application provides an air intake control method for a fanless incubator. This air intake control method can achieve precise regulation of the culture environment, keep the gas environment inside the inner chamber stable, and thus improve the yield of cell culture.

[0075] Specifically, please refer to Figure 6 The air intake control method for the fanless incubator of this application includes the following steps:

[0076] S1: Obtain the target oxygen concentration and initial oxygen concentration inside the inner liner 2.

[0077] Specifically, an oxygen sensor is installed inside the inner liner 2 to detect the oxygen concentration inside the inner liner 2. The target oxygen concentration is the oxygen concentration required for cell culture. This value is pre-input into the controller before starting use and can be directly obtained from the controller. The initial oxygen concentration is the oxygen concentration inside the inner liner 2 detected by the oxygen sensor when it is first started.

[0078] S2: Subtract the target oxygen concentration from the initial oxygen concentration to obtain the oxygen concentration difference.

[0079] S3: Obtain the target carbon dioxide concentration and initial carbon dioxide concentration inside the inner liner 2.

[0080] Specifically, a carbon dioxide sensor is installed inside the inner liner 2 to detect the concentration of carbon dioxide within it. The target carbon dioxide concentration is the concentration required for cell culture. This value is pre-input into the controller before startup and can be directly obtained from the controller. The initial carbon dioxide concentration is the concentration of carbon dioxide in the inner liner 2 detected by the carbon dioxide sensor when the device is first started.

[0081] S4: Subtract the target carbon dioxide concentration from the initial carbon dioxide concentration to obtain the carbon dioxide concentration difference.

[0082] S5: Selectively execute rapid intake mode or fine-tuned intake mode based on the difference in oxygen concentration and the difference in carbon dioxide concentration.

[0083] In the rapid intake mode, the total intake volume is greater than that in the fine-tuning intake mode.

[0084] The air intake control method of the fanless incubator of this application includes obtaining the target oxygen concentration, initial oxygen concentration, target carbon dioxide concentration, and initial carbon dioxide concentration inside the inner chamber 2; subtracting the target oxygen concentration from the initial oxygen concentration to obtain the oxygen concentration difference; subtracting the target carbon dioxide concentration from the initial carbon dioxide concentration to obtain the carbon dioxide concentration difference; and selectively executing a rapid air intake mode or a fine-tuning air intake mode based on the oxygen concentration difference and the carbon dioxide concentration difference. This setting method selects an air intake mode that matches the current inner chamber environment based on the oxygen concentration difference and the carbon dioxide concentration difference, so that the gas concentration inside the inner chamber can be quickly restored to the target set value, which is convenient for use.

[0085] It should be noted that this application does not impose any restrictions on the specific steps of step S5. In practical applications, those skilled in the art can set the specific execution steps of step S5 according to actual needs. For example, a rapid air intake mode or a fine-tuning air intake mode can be selectively executed based on the magnitude of the oxygen concentration difference and the carbon dioxide concentration difference. Adjustments and changes to the execution steps of step S5 do not deviate from the basic principles of this application.

[0086] In a preferred embodiment, please refer to Figure 7 Step S5 specifically includes:

[0087] S51: Determine whether the oxygen concentration difference is greater than the first preset difference.

[0088] The oxygen concentration difference is compared with a first preset difference. If the oxygen concentration difference is greater than the first preset difference, step S53 is executed. If the oxygen concentration difference is not greater than the first preset difference, step S52 is executed.

[0089] S52: Determine whether the absolute value of the carbon dioxide concentration difference is greater than the first preset difference value.

[0090] The absolute value of the carbon dioxide concentration difference is compared with the first preset difference. If the absolute value of the carbon dioxide concentration difference is greater than the first preset difference, step S53 is executed. If the absolute value of the carbon dioxide concentration difference is not greater than the first preset difference, step S54 is executed.

[0091] S53: Execute rapid air intake mode.

[0092] If the oxygen concentration difference is greater than the first preset difference, it means that the oxygen content in the inner liner 2 needs to be reduced quickly to decrease the oxygen concentration. If the absolute value of the carbon dioxide concentration difference is greater than the first preset difference, it means that the carbon dioxide content in the inner liner 2 needs to be increased quickly to increase the carbon dioxide concentration. In this case, the rapid air intake mode is executed, which can adjust the air intake volume by controlling the air intake flow rate and / or air intake time, thereby quickly converting the environment in the inner liner 2 into the required gas environment.

[0093] S54: Execute fine-tuning intake mode.

[0094] If the difference in oxygen concentration is not greater than the first preset difference and the difference in carbon dioxide concentration is not greater than the first preset difference, it means that the gas environment inside the inner liner 2 is close to the required environment, and the fine-tuning air intake mode is executed.

[0095] It should be noted that this application does not impose any restrictions on the specific value of the first preset difference. In practical applications, those skilled in the art can set the value of the first preset difference according to actual needs and input it into the controller. For example, the first preset difference may be 0.5%, or the first preset difference may be 2%, etc. Adjustments and changes to the value of the first preset difference do not deviate from the basic principles of this application and should be limited to the protection scope of this application.

[0096] Preferably, the first preset difference is not less than 1%.

[0097] In a preferred embodiment, please refer to Figure 8 Step S53, "Execute rapid intake mode", specifically includes:

[0098] S531: Determine the target carbon dioxide intake volume and the target nitrogen intake volume based on the target carbon dioxide concentration, the initial carbon dioxide concentration, the target oxygen concentration, the initial oxygen concentration, and the volume of the inner liner 2.

[0099] Preferably, step S531 includes:

[0100] S5311: According to formula V CO2 =(c 1CO2 -c 2CO2 The target carbon dioxide intake volume is calculated as V × V, where V CO2 The target carbon dioxide intake volume, c 1CO2 It is the target carbon dioxide concentration, c 2CO2 V is the initial carbon dioxide concentration, and V is the volume of the inner liner.

[0101] S5312: According to formula V N2 ×c 2O2 =(c 2O2 -c 1O2 The target nitrogen intake volume is calculated as V × V, where V N2 The target nitrogen intake volume, c 1O2 It is the target oxygen concentration, c 2O2 This is the initial oxygen concentration.

[0102] S532: Determine whether the target carbon dioxide intake is less than the target nitrogen intake.

[0103] The target carbon dioxide intake volume is compared with the target nitrogen intake volume to determine their relative values, so that the first intake mode or the second intake mode can be selectively executed based on the determination result. Specifically, if the target carbon dioxide intake volume is not less than the target nitrogen intake volume, step S533 is executed; if the target carbon dioxide intake volume is less than the target nitrogen intake volume, step S534 is executed.

[0104] S533: Execute the first intake mode, wherein the first intake mode is to introduce only carbon dioxide in the first stage and to introduce both carbon dioxide and nitrogen in the second stage.

[0105] If the target carbon dioxide intake is not less than the target nitrogen intake, it means that the amount of carbon dioxide introduced into inner liner 2 is greater than the amount of nitrogen introduced into inner liner 2. At this time, the first intake mode is executed, first introducing carbon dioxide, and then introducing carbon dioxide and nitrogen.

[0106] S534: Execute the second intake mode, wherein the second intake mode is that only nitrogen is introduced in the first stage and only carbon dioxide is introduced in the second stage.

[0107] If the target carbon dioxide intake is less than the target nitrogen intake, it means that the amount of nitrogen introduced into inner liner 2 should be greater than the amount of carbon dioxide introduced into inner liner 2. In this case, the second intake mode is executed, introducing nitrogen first and then carbon dioxide.

[0108] When executing the rapid air intake mode, this application first determines the target carbon dioxide intake volume and the target nitrogen intake volume. When the target carbon dioxide intake volume is not less than the target nitrogen intake volume, the first air intake mode is executed, which introduces carbon dioxide first, followed by nitrogen and carbon dioxide. When the target carbon dioxide intake volume is less than the target nitrogen intake volume, the second air intake mode is executed, which introduces nitrogen first, followed by carbon dioxide. This avoids gas waste, saves costs, and enables the gas environment inside the inner liner 2 to be converted to the required gas environment more quickly.

[0109] Preferably, please refer to Figure 9 Step S533, "Execute the first intake mode", specifically includes:

[0110] S5331: Control the intake device 9 to introduce carbon dioxide into the inner liner 2.

[0111] Specifically, the intake solenoid valve 9311 and the first solenoid valve 9321 are opened, and the second solenoid valve 9331 is closed, so that the first intake pipe 932 is connected and the second intake pipe 933 is disconnected, so that the exhaust pipe 931 only introduces carbon dioxide into the inner liner 2.

[0112] S5332: Real-time detection of carbon dioxide concentration inside the inner liner 2, recorded as the actual carbon dioxide concentration.

[0113] S5333: When the actual carbon dioxide concentration reaches the set carbon dioxide concentration, the air intake device 9 is controlled to introduce carbon dioxide and nitrogen into the inner liner 2; wherein the set carbon dioxide concentration is not higher than the target carbon dioxide concentration.

[0114] When the actual carbon dioxide concentration inside the inner liner 2 rises to near the target carbon dioxide concentration, i.e., when the set carbon dioxide concentration is reached, the intake device 9 is controlled to introduce carbon dioxide and nitrogen into the inner liner 2, thereby further reducing the oxygen concentration. This is to adjust the oxygen concentration inside the inner liner 2 to the target oxygen concentration. Simultaneously introducing nitrogen and carbon dioxide prevents the previously introduced carbon dioxide from being discharged, thus maintaining a relatively stable carbon dioxide concentration inside the inner liner 2. Specifically, the second solenoid valve 9331 is opened, connecting the second intake pipe 933, thereby allowing the outlet pipe 931 to simultaneously introduce carbon dioxide and nitrogen into the inner liner 2.

[0115] S5334: Real-time detection of oxygen concentration inside the inner liner 2, recorded as the actual oxygen concentration.

[0116] The oxygen concentration inside the inner liner 2 is monitored in real time to determine whether the gas environment inside the inner liner 2 has been converted to the required gas environment.

[0117] S5335: When the actual oxygen concentration drops to the target oxygen concentration, the first intake mode ends and the fine-tuning intake mode is executed.

[0118] When the actual oxygen concentration drops to the target oxygen concentration, it indicates that the gas environment inside the inner liner 2 has been converted to the required gas environment. The first air intake mode ends and the fine-tuning air intake mode is executed to keep the gas environment stable and allow the cells to be cultured stably.

[0119] It should be noted that this application does not impose any restrictions on the value of the carbon dioxide concentration. In practical applications, those skilled in the art can determine the value of the carbon dioxide concentration according to actual needs, as long as the carbon dioxide concentration is close to the target carbon dioxide value. Any adjustments or changes to the value of the carbon dioxide concentration do not deviate from the basic principles of this application and should be limited to the protection scope of this application.

[0120] In one embodiment, the carbon dioxide concentration is set to be 0.1% lower than the target carbon dioxide concentration.

[0121] In another embodiment, the carbon dioxide concentration is set to be equal to the target carbon dioxide concentration.

[0122] In one embodiment, the flow rate of carbon dioxide in step S5331 is greater than that in step S5333 to avoid carbon dioxide overshoot during the simultaneous introduction of carbon dioxide and nitrogen. The optimal flow rate ratio of carbon dioxide to nitrogen in step S5333 can be determined experimentally.

[0123] In another embodiment, the flow rate of carbon dioxide in step S5331 is the same as that in step S5333. In step S5333, carbon dioxide is introduced intermittently while nitrogen is continuously introduced, thereby avoiding carbon dioxide overshoot during the simultaneous introduction of carbon dioxide and nitrogen. The timing and time interval of carbon dioxide introduction in step S5333 can be determined experimentally.

[0124] Preferably, please refer to Figure 10 The specific steps for "executing the second air intake mode" include:

[0125] S5341: Control the intake device 9 to introduce nitrogen into the inner liner 2.

[0126] Specifically, the intake solenoid valve 9311 and the second solenoid valve 9331 are opened, and the first solenoid valve 9321 is closed, which disconnects the first intake pipe 932 and connects the second intake pipe 933, so that the exhaust pipe 931 only introduces nitrogen into the inner liner 2.

[0127] S5342: Real-time detection of oxygen concentration inside the inner liner 2, recorded as the actual oxygen concentration.

[0128] S5343: Determine whether the nitrogen gas supply cutoff point has been reached based on the target carbon dioxide concentration, target oxygen concentration, and actual oxygen concentration, and when the nitrogen gas supply cutoff point is reached, control the air intake device 9 to supply carbon dioxide into the inner liner 2.

[0129] As nitrogen is introduced, the oxygen concentration inside the inner liner 2 continuously decreases. During the nitrogen introduction process, the target carbon dioxide concentration, target oxygen concentration, and actual oxygen concentration are used to determine whether the nitrogen introduction cutoff point has been reached. When the nitrogen introduction cutoff point is reached, the nitrogen introduction is stopped, and the air intake device 9 is controlled to introduce carbon dioxide into the inner liner 2. During this process, the carbon dioxide concentration continuously increases, while the oxygen concentration further decreases. Specifically, the second solenoid valve 9331 is opened, which also connects the second air intake pipe 933, thereby allowing the air outlet pipe 931 to simultaneously introduce carbon dioxide and nitrogen into the inner liner 2.

[0130] S5344: Real-time detection of carbon dioxide concentration inside the inner liner 2, recorded as the actual carbon dioxide concentration.

[0131] S5345: When the actual carbon dioxide concentration rises to the target carbon dioxide concentration, the second intake mode ends and the fine-tuning intake mode is executed.

[0132] When the actual carbon dioxide concentration rises to the target carbon dioxide concentration, it indicates that the gas environment inside the inner liner 2 is close to the gas environment required for cultivation. At this point, the second air intake mode is terminated and the fine-tuning air intake mode is executed to keep the gas environment inside the inner liner 2 stable.

[0133] Preferably, step S5343 specifically includes:

[0134] S53431: Determine (1-c) 1CO2 )×c 2O2 -c 1O2 Is it less than 0.1%?

[0135] S53432: If the judgment result is "yes", it is determined that the nitrogen gas supply cutoff point has been reached, and the control air intake device 9 is executed to introduce carbon dioxide into the inner liner 2.

[0136] S53433: If the judgment result is "no", it is determined that the nitrogen gas supply cutoff point has not been reached, and then return to step S53431.

[0137] In this implementation, by determining (1-c) 1CO2 )×c 2O2 -c 1O2The nitrogen purging stop point is determined by whether the nitrogen concentration is less than 0.1%. If it is less than 0.1%, it means that if nitrogen is continued to be introduced at this point, the subsequent introduction of carbon dioxide will lead to an oxygen imbalance (lower than the target oxygen concentration) after the carbon dioxide concentration reaches the target. Therefore, nitrogen purging is stopped, and carbon dioxide purging begins. If the nitrogen concentration is not less than 0.1%, it means that the oxygen concentration is still far from the target oxygen concentration, and nitrogen can be continued to further reduce the oxygen concentration. This setting method can quickly reduce the oxygen concentration and avoid the oxygen concentration falling below the target oxygen concentration when carbon dioxide is introduced later. It can better convert the gas environment in the inner liner 2 to the target gas environment and facilitates control.

[0138] Preferably, please refer to Figure 11 Step S54, "Execute fine-tuning of intake mode," specifically includes:

[0139] S541: Real-time acquisition of the actual oxygen concentration and actual carbon dioxide concentration inside the inner liner 2.

[0140] S542: Subtract the target oxygen concentration from the actual oxygen concentration to obtain the real-time oxygen concentration difference.

[0141] S543: Subtract the target carbon dioxide concentration from the actual carbon dioxide concentration to obtain the real-time carbon dioxide concentration difference.

[0142] S544: Based on the real-time oxygen concentration difference and / or the real-time carbon dioxide concentration difference, selectively control the air intake device 9 to introduce nitrogen and / or carbon dioxide into the inner liner 2.

[0143] By selectively controlling the supply of nitrogen and / or carbon dioxide into the inner liner 2 through the real-time oxygen concentration difference and / or real-time carbon dioxide concentration difference, the oxygen and carbon dioxide concentrations in the inner liner 2 are adjusted in real time to keep them within an acceptable fluctuation range of the target concentration, thereby stabilizing the gas environment in the inner liner 2, avoiding oxygen and carbon dioxide overshoot, and effectively improving the yield of cell culture.

[0144] It should be noted that this application does not impose any restrictions on the specific execution steps of step S544. In practical applications, those skilled in the art can set the specific execution steps of step S544 according to actual needs. Adjustments and changes to the execution steps of step S544 do not deviate from the basic principles of this application and should be limited to the protection scope of this application.

[0145] In one embodiment, step S544, "selectively controlling the air intake device 9 to introduce nitrogen and / or carbon dioxide into the inner liner 2 based on the real-time oxygen concentration difference and / or the real-time carbon dioxide concentration difference," specifically includes:

[0146] S5441: Compare the real-time oxygen concentration difference with the second preset difference.

[0147] The real-time oxygen concentration difference is compared with the second preset difference to determine the magnitude of the difference. If the real-time oxygen concentration difference is not less than the second preset difference, step S5442 is executed to control the air intake device 9 to introduce nitrogen into the inner liner 2. If the real-time oxygen concentration difference is less than the second preset difference, step S5443 is executed to stop controlling the air intake device 9 to introduce nitrogen into the inner liner 2.

[0148] S5442: Controls the intake device 9 to introduce nitrogen into the inner liner 2.

[0149] If the real-time oxygen concentration difference is not less than the second preset difference, it means that the oxygen concentration in the inner liner 2 is outside the allowable fluctuation range. At this time, the oxygen concentration exceeds the target oxygen concentration. Therefore, nitrogen needs to be introduced into the inner liner 2 to reduce the oxygen concentration in the inner liner 2.

[0150] S5443: If the real-time oxygen concentration difference is less than the second preset difference, then the intake device 9 will not be controlled to introduce nitrogen into the inner liner 2.

[0151] If the real-time oxygen concentration difference is less than the second preset difference, it means that the oxygen concentration in the inner liner 2 is within the allowable fluctuation range. At this time, the air intake device 9 is not controlled to introduce nitrogen into the inner liner 2. Specifically, if nitrogen is being introduced, the introduction is stopped; if nitrogen is not being introduced, the state is maintained.

[0152] It should be noted that this application does not impose any restrictions on the specific value of the second preset difference. In practical applications, those skilled in the art can determine the value of the second preset difference according to actual needs. For example, when the oxygen concentration suitable for cell growth is 4.7% to 5.2%, the target oxygen concentration can be set to 5%, and the second preset difference can be 0.1% or 0.15%. Any adjustments or changes to the second preset difference do not deviate from the basic principles of this application and should be limited to the scope of protection of this application.

[0153] Preferably, the second preset difference is 0.1% to 0.2%.

[0154] In another embodiment, step S544, "selectively controlling the air intake device 9 to introduce nitrogen and / or carbon dioxide into the inner liner 2 based on the real-time oxygen concentration difference and / or the real-time carbon dioxide concentration difference," specifically includes:

[0155] S5441: Compare the absolute value of the real-time carbon dioxide concentration difference with the third preset difference value.

[0156] The absolute value of the real-time carbon dioxide concentration difference is compared with the third preset difference. The magnitude of the real-time carbon dioxide concentration difference is determined. If the real-time carbon dioxide concentration difference is less than the third preset difference, step S5442 is executed, and the air intake device 9 is not controlled to introduce carbon dioxide into the inner liner 2. If the absolute value of the real-time carbon dioxide concentration difference is not less than the third preset difference, step S5443 is executed to further determine whether the real-time carbon dioxide concentration difference is greater than 0.

[0157] S5442: The intake device 9 is not controlled to allow carbon dioxide to enter the inner liner 2.

[0158] If the absolute value of the real-time carbon dioxide concentration difference is less than the third preset difference, it means that the carbon dioxide concentration in the inner liner 2 is within the allowable fluctuation range, and there is no need to introduce carbon dioxide into the inner liner 2 further.

[0159] S5443: Then determine whether the real-time carbon dioxide concentration difference is greater than 0.

[0160] If the absolute value of the real-time carbon dioxide concentration difference is not less than the third preset difference, the real-time carbon dioxide concentration difference is further compared with 0 to determine whether the carbon dioxide concentration is too high or too low relative to the target carbon dioxide concentration, so as to perform the next step.

[0161] S5444: If the real-time carbon dioxide concentration difference is greater than 0, control the air intake device 9 to introduce nitrogen into the inner liner 2.

[0162] If the real-time carbon dioxide concentration difference is greater than 0, it means that the actual carbon dioxide concentration is too high relative to the target carbon dioxide concentration. In this case, it is necessary to reduce the carbon dioxide concentration, so control the air intake device 9 to introduce nitrogen into the inner liner 2.

[0163] S5445: If the real-time carbon dioxide concentration difference is less than 0, control the intake device 9 to introduce carbon dioxide into the inner liner 2.

[0164] If the real-time carbon dioxide concentration difference is less than 0, it means that the actual carbon dioxide concentration is too low relative to the target carbon dioxide concentration. In this case, it is necessary to increase the carbon dioxide concentration, so control the air intake device 9 to introduce carbon dioxide into the inner liner 2.

[0165] It should be noted that this application does not impose any restrictions on the specific value of the third preset difference. In practical applications, those skilled in the art can determine the value of the third preset difference according to actual needs. For example, when the carbon dioxide concentration suitable for cell growth is 4.7% to 5.2%, the target carbon dioxide concentration can be set to 5%, and the third preset difference can be 0.1% or 0.15%. Any adjustments or changes to the third preset difference do not deviate from the basic principles of this application and should be limited to the scope of protection of this application.

[0166] Preferably, the third preset difference is 0.1% to 0.2%.

[0167] Preferably, when it is necessary to open the air intake solenoid valve 9311 to allow air intake device 9 to ventilate into inner liner 2, the air intake control method of the fanless incubator further includes the following steps:

[0168] Obtain the gas pressure detected by the electromagnetic pressure sensor 9312;

[0169] Compare the gas pressure with the set pressure value;

[0170] The set pressure value is pre-stored in the controller, and its specific value is the minimum pressure value that can blow the impeller 92, which is determined through experiments.

[0171] If the gas pressure is lower than the set pressure value, the pressure supplied by the nitrogen and / or carbon dioxide gas sources connected to the intake pipe 931 will be increased.

[0172] If the gas pressure is not less than the set pressure value, the intake solenoid valve 9311 is opened to allow the intake device 9 to vent air into the inner liner 2.

[0173] This setup involves checking the pressure of the intake solenoid valve 9311 before actual ventilation to ensure that the outlet pressure is high enough to smoothly drive the impeller 92 to rotate. This ensures that the gas environment inside the inner liner 2 is uniformly and stably converted to the target environment, preventing over-adjustment of carbon dioxide and oxygen inside the inner liner 2.

[0174] The technical solutions of this application have been described above with reference to the preferred embodiments shown in the accompanying drawings. However, it will be readily understood by those skilled in the art that the scope of protection of this application is obviously not limited to these specific embodiments. Without departing from the principles of this application, those skilled in the art can make equivalent changes or substitutions to the relevant technical features, and the technical solutions after these changes or substitutions will all fall within the scope of protection of this application.

Claims

1. A fanless incubator, characterized in that, The fanless incubator includes a box body (1), an inner liner (2) located inside the box body (1), and an air intake device (9) installed inside the inner liner (2). The air intake device (9) is configured to first extract a portion of the air in the inner liner (2) and mix it with the target gas when the target gas is introduced into the inner liner (2), and then introduce the mixture of air and target gas into the inner liner (2) so that the target gas can be quickly diffused to each area of ​​the inner liner (2), thereby keeping the concentration of the target gas in each area of ​​the inner liner (2) uniform.

2. The fanless incubator according to claim 1, characterized in that, The air intake device (9) includes a mixing housing (91), an impeller (92) disposed within the mixing housing (91), and an air intake piping system (93) penetrating the mixing housing (91). The hybrid housing (91) has a first sidewall (911) and a second sidewall (912) disposed opposite to each other, and an annular sidewall (913) connecting the first sidewall (911) and the second sidewall (912). The first sidewall (911) is installed on the top wall of the inner liner (2), the impeller (92) is rotatably installed on the top wall of the inner liner (2), the second sidewall (912) is provided with an air inlet (9121), the annular sidewall (913) is provided with a plurality of mixed gas outlets (9131), and the outlet of the air outlet pipe (931) of the air inlet pipe system (93) is provided on the circumferential outer side of the impeller (92) facing the impeller (92).

3. The fanless incubator according to claim 2, characterized in that, The hybrid shell (91) is cylindrical; And / or, the cross-sectional area of ​​the air inlet (9121) is 30% to 70% of the total area of ​​the second sidewall (912); And / or, the total cross-sectional area of ​​the plurality of said mixed gas outlets (9131) is 50% to 80% of the total area of ​​said annular sidewall (913); And / or, the intake piping system (93) includes an exhaust piping (931), a first intake piping (932), a second intake piping (933), a carbon dioxide source, and a nitrogen source. The two ends of the first intake piping (932) are respectively connected to the carbon dioxide source and the inlet of the exhaust piping (931), and the two ends of the second intake piping (933) are respectively connected to the nitrogen source and the inlet of the exhaust piping (931).

4. The fanless incubator according to claim 3, characterized in that, The air outlet pipe (931) is equipped with an air intake solenoid valve (9311) and an electromagnetic pressure sensor (9312). The electromagnetic pressure sensor (9312) is used to detect the pressure at the port of the air intake solenoid valve (9311) when the air intake solenoid valve (9311) is closed. And / or, a first solenoid valve (9321) is provided on the first intake pipe (932); And / or, a first butterfly filter (9322) is provided on the first intake pipe (932), and the first butterfly filter (9322) is located between the first solenoid valve (9321) and the carbon dioxide gas source; And / or, a second solenoid valve (9331) is provided on the second intake pipe (933); And / or, a second butterfly filter (9332) is provided on the second air intake pipe (933), the second butterfly filter (9332) being located between the second solenoid valve (9331) and the nitrogen source.

5. The fanless incubator according to any one of claims 1 to 4, characterized in that, The fanless incubator also includes a positive pressure exhaust valve, through which the environment inside the inner liner (2) is selectively connected to the external environment.

6. The fanless incubator according to claim 5, characterized in that, The air intake device (9) is installed on the top wall of the inner liner (2), and the positive pressure exhaust valve is located in the bottom area of ​​the side wall of the inner liner (2); And / or, the number of positive pressure exhaust valves is two, and the two positive pressure exhaust valves are respectively disposed on two opposite side walls of the inner liner (2).

7. An air intake control method for a fanless incubator, wherein the fanless incubator is the fanless incubator according to any one of claims 1 to 6, characterized in that, The intake control method includes: Obtain the target oxygen concentration and initial oxygen concentration inside the inner liner (2); Subtracting the target oxygen concentration from the initial oxygen concentration yields the oxygen concentration difference. Obtain the target carbon dioxide concentration and initial carbon dioxide concentration inside the inner liner (2); Subtracting the target carbon dioxide concentration from the initial carbon dioxide concentration yields the carbon dioxide concentration difference. Based on the oxygen concentration difference and the carbon dioxide concentration difference, a rapid air intake mode or a fine-tuning air intake mode is selectively executed. The total air intake in the rapid air intake mode is greater than the total air intake in the fine-tuning air intake mode.

8. The air intake control method for a fanless incubator according to claim 7, characterized in that, The step of "selectively executing a rapid intake mode or a fine-tuned intake mode based on the oxygen concentration difference and the carbon dioxide concentration difference" specifically includes: Determine whether the oxygen concentration difference is greater than a first preset difference; Determine whether the absolute value of the carbon dioxide concentration difference is greater than a first preset difference; If the oxygen concentration difference is greater than the first preset difference or the absolute value of the carbon dioxide concentration difference is greater than the first preset difference, then the rapid air intake mode is executed. If the oxygen concentration difference is not greater than the first preset difference and the carbon dioxide concentration difference is not greater than the first preset difference, then the fine-tuning intake mode is executed.

9. The air intake control method for a fanless incubator according to claim 8, characterized in that, The steps for "executing the rapid air intake mode" specifically include: The target carbon dioxide intake and the target nitrogen intake are determined based on the target carbon dioxide concentration, the initial carbon dioxide concentration, the target oxygen concentration, the initial oxygen concentration, and the volume of the inner liner (2). Compare the target carbon dioxide intake volume with the target nitrogen intake volume; If the target carbon dioxide intake volume is not less than the target nitrogen intake volume, then the first intake mode is executed; If the target carbon dioxide intake is less than the target nitrogen intake, then the second intake mode is executed; The first air intake mode is that only carbon dioxide is introduced in the first stage, and both carbon dioxide and nitrogen are introduced in the second stage; the second air intake mode is that only nitrogen is introduced in the first stage, and only carbon dioxide is introduced in the second stage. And / or, the step of "executing the fine-tuned intake mode" specifically includes: The actual oxygen concentration and actual carbon dioxide concentration inside the inner liner (2) are obtained in real time; Subtracting the target oxygen concentration from the actual oxygen concentration yields the real-time oxygen concentration difference. Subtracting the target carbon dioxide concentration from the actual carbon dioxide concentration yields the real-time carbon dioxide concentration difference. Based on the real-time oxygen concentration difference and / or the real-time carbon dioxide concentration difference, the air intake device (9) is selectively controlled to introduce nitrogen and / or carbon dioxide into the inner liner (2).

10. The air intake control method for a fanless incubator according to claim 9, characterized in that, The step of "determining the target carbon dioxide intake and the target nitrogen intake based on the target carbon dioxide concentration, the initial carbon dioxide concentration, the target oxygen concentration, the initial oxygen concentration, and the volume of the inner liner (2)" specifically includes: According to formula V CO2 =(c 1CO2 -c 2CO2 The target carbon dioxide intake volume is calculated as V × V, where V CO2 The target carbon dioxide intake volume, c 1CO2 It is the target carbon dioxide concentration, c 2CO2 V is the initial carbon dioxide concentration, and V is the volume of the inner liner. According to formula V N2 ×c 2O2 =(c 2O2 -c 1O2 The target nitrogen intake volume is calculated as V × V, where V N2 The target nitrogen intake volume, c 1O2 It is the target oxygen concentration, c 2O2 It is the initial oxygen concentration; And / or, The specific steps for "executing the first intake mode" include: Control the air intake device (9) to introduce carbon dioxide into the inner liner (2); The concentration of carbon dioxide inside the inner liner (2) is detected in real time and recorded as the actual carbon dioxide concentration; When the actual carbon dioxide concentration reaches the set carbon dioxide concentration, the air intake device (9) is controlled to introduce carbon dioxide and nitrogen into the inner liner (2); wherein the set carbon dioxide concentration is not higher than the target carbon dioxide concentration; The oxygen concentration inside the inner liner (2) is detected in real time and recorded as the actual oxygen concentration; When the actual oxygen concentration decreases to the target oxygen concentration, the first intake mode ends and the fine-tuning intake mode is executed. And / or, The steps for "executing the second intake mode" specifically include: The air intake device (9) is controlled to introduce nitrogen gas into the inner liner (2); The oxygen concentration inside the inner liner (2) is detected in real time and recorded as the actual oxygen concentration; Based on the target carbon dioxide concentration, the target oxygen concentration and the actual oxygen concentration, it is determined whether the nitrogen gas supply cutoff point has been reached, and when the nitrogen gas supply cutoff point is reached, the air intake device (9) is controlled to supply carbon dioxide into the inner liner (2); The concentration of carbon dioxide inside the inner liner (2) is detected in real time and recorded as the actual carbon dioxide concentration; When the actual carbon dioxide concentration rises to the target carbon dioxide concentration, the second intake mode ends and the fine-tuning intake mode is executed. And / or, The step of "selectively controlling the air intake device (9) to introduce nitrogen and / or carbon dioxide into the inner liner (2) based on the real-time oxygen concentration difference and / or the real-time carbon dioxide concentration difference" specifically includes: The real-time oxygen concentration difference is compared with a second preset difference. If the real-time oxygen concentration difference is not less than the second preset difference, then control the air intake device (9) to introduce nitrogen into the inner liner (2); If the real-time oxygen concentration difference is less than the second preset difference, then the air intake device (9) is not controlled to introduce nitrogen into the inner liner (2); And / or, The step of "selectively controlling the air intake device (9) to introduce nitrogen and / or carbon dioxide into the inner liner (2) based on the real-time oxygen concentration difference and / or the real-time carbon dioxide concentration difference" specifically includes: The absolute value of the real-time carbon dioxide concentration difference is compared with a third preset difference value; If the absolute value of the real-time carbon dioxide concentration difference is less than the third preset difference, then the air intake device (9) is not controlled to introduce carbon dioxide into the inner liner (2); If the absolute value of the real-time carbon dioxide concentration difference is not less than the third preset difference, then determine whether the real-time carbon dioxide concentration difference is greater than 0; If the real-time carbon dioxide concentration difference is greater than 0, then control the air intake device (9) to introduce nitrogen into the inner liner (2); If the real-time carbon dioxide concentration difference is less than 0, the air intake device (9) is controlled to introduce carbon dioxide into the inner liner (2).

11. The air intake control method for a fanless incubator according to claim 10, characterized in that, The step of "determining whether the nitrogen cutoff point has been reached based on the target carbon dioxide concentration, the target oxygen set concentration, and the actual oxygen concentration" specifically includes: Determine (1-c) 1CO2 )×c 2O2 -c 1O2 Is it less than 0.1%? If the judgment result is "yes", then it is determined that the nitrogen gas purging cutoff point has been reached; If the judgment result is "no", it is determined that the nitrogen gas supply cutoff point has not been reached; And / or, the set carbon dioxide concentration is 0.1% lower than the target carbon dioxide concentration; And / or, the first preset difference is not less than 1%; And / or, the second preset difference is 0.1% to 0.2%; And / or, the third preset difference is 0.1% to 0.2%.

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