Full-automatic electrostatic spinning membrane treatment and cell culture partitioning device

Abstract

The invention discloses a full-automatic electrostatic spinning membrane treatment and cell culture zoning device, relates to the technical field of biological manufacturing, and aims to solve the problems of limitation of multi-process integration and automatic operation and cell culture zoning in the prior art. Comprising a Z-axis lifting seat, a chip, a supporting mechanism, an electrostatic spinning mechanism and a clamping mechanism, a printing pit slot is formed in the top surface of the chip and used for printing a structural layer material; the supporting mechanism controls the telescopic rod to extend above the structural layer material; a spinning receiving frame of the electrostatic spinning mechanism can move, and a telescopic rod is covered with a film; the clamping mechanism folds and compresses the film, and after the functional material layer is printed on the top surface of the film, the chip can be detached for co-culture. According to the invention, automatic integration of electrostatic spinning, membrane treatment and cell culture zoning is realized, the operation precision and efficiency are improved, the zoning problem in cell culture is solved, cross contamination is effectively avoided, the accuracy and reliability of experimental results are improved, and powerful support is provided for construction of a complex tissue system.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biofabrication, and more particularly to a full-automatic electrospinning membrane processing and cell culture partition device. BACKGROUND

[0002] With the continuous development of biofabrication technology, electrospinning, 3D bioprinting and other technologies have been widely used in cell culture, tissue engineering and other fields. However, the existing technology still has many limitations in multi-process integration and automated operation.

[0003] On the one hand, electrospinning technology is usually used to prepare nanofiber membranes, which can be used for cell scaffolds or drug delivery. However, after the spinning is completed, the membrane material needs to be manually transported to the target position, which not only leads to inaccurate operation, but also low efficiency. In addition, the existing equipment lacks automation in process conversion and membrane material processing, increasing the risk of human interference and affecting the accuracy and reliability of experimental results.

[0004] On the other hand, the partition problem in cell culture is also a technical difficulty. Especially when different cells need to be cultured in upper and lower chambers, how to achieve precise sealing and isolation to avoid cross contamination is a problem that existing equipment cannot solve. At present, most of the equipment generally relies on manual adjustment when partitioning cell culture, and cannot realize full automation, which greatly limits the application and development of biofabrication technology in complex tissue system construction.

[0005] Therefore, it is urgent to develop a biofabrication device that can realize multi-process integration, automated operation and precise cell culture partition. SUMMARY

[0006] Therefore, the present application provides a full-automatic electrospinning membrane processing and cell culture partition device to solve the above technical problems.

[0007] In order to achieve the above purpose, the present application adopts the following technical solutions:

[0008] A full-automatic electrospinning membrane processing and cell culture partition device, comprising:

[0009] A Z-axis lifting seat;

[0010] A chip, which is detachably connected to the Z-axis lifting seat, has a printing pit on the top surface, and the bottom surface of the printing pit is used for printing a structural layer material;

[0011] A supporting mechanism is installed on the chip and located on one side of the printing pit, and when the structural layer material is printed on the bottom surface of the printing pit, the supporting mechanism controls the extension of the telescopic rod, and the telescopic rod is located above the structural layer material;

[0012] An electrospinning mechanism is provided outside the Z-axis lifting seat, and the electrospinning mechanism has a movable spinning receiving frame, when the telescopic rod is extended, the spinning receiving frame moves above the printing pit, and the film on the spinning receiving frame is covered on the upper surface of the telescopic rod;

[0013] A clamping mechanism is detachably connected on both sides of the chip, when the film is completed, the clamping plate of the clamping mechanism is folded and pressed outside the printing pit, and cooperates with the telescopic rod to press the film, and finally prints a functional material layer on the top surface of the film; after the above operations are completed, the chip is detached from the Z-axis lifting seat, and the subsequent culture medium is injected for co-culture.

[0014] Through the above technical solutions, the electrospinning, film processing and cell culture partition and other processes are integrated, and the automatic operation is realized through the cooperation of each mechanism, the work efficiency is improved, the errors and interference caused by human operation are reduced, and the accuracy and reliability of the experimental results are improved. Through the cooperation of the supporting mechanism, the electrospinning mechanism and the clamping mechanism, the precise covering and pressing of the film are realized, so that the precise sealing and isolation of the upper and lower chambers during cell culture are realized, cross contamination is effectively avoided, and the construction of complex tissue system is facilitated.

[0015] Preferably, in the above-mentioned full-automatic electrospinning film processing and cell culture partition device, the top surface of the chip has a mounting pit groove on one side of the printing pit groove, a plurality of parallelly arranged telescopic rods are slidably arranged in the mounting pit groove, the bottom surface of the mounting pit groove is slidably connected with a sliding seat, the sliding seat has a plurality of semicircular grooves on it which are fitted with the ends of the plurality of telescopic rods, the edge of the mounting pit groove close to the printing pit groove has a first convex edge, the first convex edge has a plurality of semicircular grooves on it which are fitted with the plurality of telescopic rods, when the telescopic rods are not extended, the sliding seat is located on the side of the mounting pit groove away from the first convex edge, and the two ends of the telescopic rods are fitted with the semicircular grooves on the sliding seat and the first convex edge respectively; the side of the printing pit groove away from the mounting pit groove has a second convex edge, the second convex edge has a plurality of semicircular grooves on it which are fitted with the plurality of telescopic rods, when the telescopic rods are extended, the sliding seat moves towards the first convex edge and abuts against the first convex edge, the end of the telescopic rod away from the sliding seat is fitted with the semicircular groove on the second convex edge, and the telescopic rod and the bottom surface of the printing pit groove have a gap for printing of the structural layer material.

[0016] By arranging the mounting pit groove, the telescopic rod, the sliding seat and the convex edge matched therewith on the chip, the telescopic rod is more stable and reliable in extension and retraction, and the position of the telescopic rod can be accurately controlled, which provides a better foundation for subsequent film covering and cell culture partition, and further improves the automation degree and operation precision of the device. The gap between the telescopic rod and the bottom surface of the printing pit groove for printing of the structural layer material ensures that the structural layer material can be smoothly printed on the bottom surface of the printing pit groove, guarantees the printing quality, and creates good conditions for subsequent film covering and printing of the functional material layer.

[0017] Preferably, in the above-mentioned full-automatic electrospinning film processing and cell culture partition device, a pressing member is detachably connected with the top surface of the chip and arranged on the top surface of the mounting pit groove, the bottom surface of the pressing member has a third convex edge corresponding to the first convex edge, the third convex edge has a semicircular groove fitted with the telescopic rod, and the semicircular groove on the first convex edge and the semicircular groove on the third convex edge combine to form a circular hole through which the telescopic rod passes.

[0018] The arrangement of the pressing member further fixes the telescopic rod, making it more stable during extension and retraction, and avoiding shaking or displacement of the telescopic rod due to external force or its own gravity, thereby improving the stability and reliability of the device and ensuring the smooth progress of the entire process.

[0019] Preferably, in the above-mentioned full-automatic electrospinning film processing and cell culture partition device, the bottom surface of the mounting pit groove has a sliding groove on both sides, and the sliding seat has a sliding block on both sides which slidably cooperates with the sliding groove.

[0020] The sliding fit structure of the sliding groove and the sliding block makes the sliding seat more stable and smooth during movement, avoiding the situation that the sliding seat is stuck or deviated during movement, thereby ensuring that the telescopic rod can be accurately extended and retracted, and improving the operation efficiency and operation accuracy of the device. This sliding fit structure increases the overall structural stability of the device, making the connection between components more compact, and enhancing the durability and reliability of the device during long-term use.

[0021] Preferably, in the above-mentioned full-automatic electrospinning membrane processing and cell culture partition device, the Z-axis lifting seat is detachably connected with an electromagnet mounting rack, the electromagnet mounting rack is located on the outer side of the chip close to the mounting pit, an electromagnet is mounted on the electromagnet mounting rack, and a magnet is fixed on the sliding seat; when the electromagnet is powered on, the sliding seat is driven to move by repulsive force with the magnet, and then the telescopic rod is controlled to extend.

[0022] The repulsive force between the electromagnet and the magnet is used to drive the sliding seat to move, and then the telescopic rod is controlled to extend, realizing automatic control of the telescopic rod without manual operation, further improving the automation degree of the device, reducing the labor intensity, and improving the work efficiency. The on-off control of the electromagnet is relatively simple and accurate, which can quickly and accurately realize the extension and retraction actions of the telescopic rod, improve the control accuracy and reliability of the device, and ensure the smooth progress of the whole process.

[0023] Preferably, in the above-mentioned full-automatic electrospinning membrane processing and cell culture partition device, the clamping mechanism includes two motor fixing racks which are detachably connected on both sides of the chip, a stepping motor is fixedly installed in each of the two motor fixing racks, the power output shaft of each of the two stepping motors is fixedly connected with the clamping plate, the clamping plate has a fourth convex edge on both sides of the end head, the fourth convex edge has a semicircular groove for embedding the telescopic rod, and when the clamping plate folds and presses the membrane, the semicircular groove on the fourth convex edge is embedded with the telescopic rod.

[0024] The clamping mechanism adopts the structure of motor fixing rack, stepping motor and clamping plate, and the folding and pressing of the clamping plate are realized by the driving of the stepping motor, realizing automatic clamping of the membrane without manual clamping, improving the clamping efficiency and accuracy, and ensuring the stability of the membrane in the subsequent process. The detachable motor fixing rack makes the installation and disassembly of the clamping mechanism more convenient, facilitating maintenance and replacement; at the same time, this structure also improves the overall integration and stability of the device, making the cooperation between components more compact, and enhancing the reliability of the device.

[0025] Preferably, in the above-mentioned full-automatic electrospinning membrane processing and cell culture partition device, the two sides of the printing groove are provided with through sealing grooves, and the clamping plate is embedded in the sealing grooves after being folded and compressed; and a sealing strip is embedded between the side wall and bottom surface of the sealing groove and the side wall and bottom surface of the clamping plate.

[0026] The sealing grooves and the sealing strip further improve the sealing performance of the device. When the clamping plate is folded and compressed and embedded in the sealing groove, the sealing strip can effectively fill the gap between the clamping plate and the sealing groove. Preferably, in the above-mentioned full-automatic electrospinning membrane processing and cell culture partition device, the electrospinning mechanism comprises an X-axis sliding rail, an X-axis moving seat slidably connected to the X-axis sliding rail, an X-axis lead screw rotatably connected to the X-axis sliding rail, the X-axis lead screw penetrating through the X-axis moving seat and being threadedly connected with the X-axis moving seat, and an X-axis drive motor fixed to one end of the X-axis sliding rail, the power output shaft of the X-axis drive motor being fixedly connected with one end of the X-axis lead screw.

[0027] The combination of the X-axis sliding rail, the X-axis moving seat, the X-axis lead screw and the X-axis drive motor enables the electrospinning mechanism to move flexibly in the X-axis direction, and the moving position is accurately controllable. The spinning receiving frame can be accurately moved to the specified position as needed, improving the flexibility and accuracy of the electrospinning process and providing a strong guarantee for precise membrane coverage. The movement of the spinning receiving frame is realized by motor driving, realizing automatic control and operation of the electrospinning mechanism, reducing manual intervention, improving work efficiency and operation accuracy, and further improving the automation level of the whole device.

[0028] Preferably, in the above-mentioned full-automatic electrospinning membrane processing and cell culture partition device, the X-axis moving seat is fixed with a Y-axis sliding channel, the Y-axis sliding channel is slidably connected with a Y-axis sliding block, one end of the Y-axis sliding block is fixed with the spinning receiving frame, the other end of the Y-axis sliding block is fixed with a threaded seat, the X-axis moving seat is fixed with a Y-axis drive motor, a Y-axis lead screw is fixedly connected with the power output shaft of the Y-axis drive motor, and the Y-axis lead screw is threadedly connected with the threaded seat.

[0029] The Y-axis sliding channel, the Y-axis sliding block, the Y-axis drive motor and the Y-axis lead screw provided on the X-axis moving seat enable the spinning receiving frame to not only move in the X-axis direction, but also accurately move and position in the Y-axis direction, realizing multi-directional accurate control and further improving the flexibility and accuracy of the electrospinning mechanism, which can better meet different process requirements and operation needs. Through the coordinated movement of the X-axis and the Y-axis, the membrane on the spinning receiving frame can be accurately covered on the upper surface of the telescopic rod, and the covering position is more accurate and uniform, improving the quality of membrane coverage and providing a better foundation for subsequent cell culture partition and functional material layer printing processes.

[0030] Preferably, in the above-mentioned full-automatic electrospinning membrane processing and cell culture partition device, the spinning receiving frame is a U-shaped frame body, the membrane is formed in the U-shaped opening of the spinning receiving frame, the chip has stepped edges on the outer two sides, when covering the membrane, the U-shaped opening of the spinning receiving frame spans the two sides of the print pit, and the two side supporting rods of the spinning receiving frame abut against the stepped edges on the two sides.

[0031] The spinning receiving frame adopts a U-shaped frame body, the U-shaped opening of which can better cooperate with the stepped edges of the chip, so that the membrane is more stable and flat when covering, and can be closely attached to the telescopic rod; at the same time, this structure is also conducive to the compression of the membrane by the clamping mechanism, improving the effect of membrane covering and compression, and ensuring the sealing between the upper and lower chambers. This structural design enables the device to better adapt to chips of different sizes and shapes, improves the versatility and application range of the device, reduces production and use costs, and is conducive to the popularization and application of the device.

[0032] Through the above technical solution, compared with the prior art, the present application provides a full-automatic electrospinning membrane processing and cell culture partition device, which has the following beneficial effects:

[0033] 1. High automation: realizes full-process automation operation from electrospinning to membrane processing and then to cell culture partition, reduces manual intervention, and improves operation efficiency and precision.

[0034] 2. Precise cell culture partition: through the unique design of the supporting mechanism, electrospinning mechanism and clamping mechanism, precise sealing and isolation of the upper and lower chambers during cell culture are realized, cross contamination is effectively avoided, and the construction of complex tissue systems is facilitated.

[0035] 3. Improve sealing and stability: the device adopts sealing design and stable mechanical structure at multiple positions, enhancing the overall sealing and running stability.

[0036] 4. Enhance operation flexibility: the electrospinning mechanism can accurately move and position in multiple directions, improving the accuracy and uniformity of membrane covering, and the optimized structural design improves the adaptability and versatility of the device to different chips.

[0037] 5. Improve the reliability of experimental results: through automation and precise control, human error is reduced, improving the accuracy and reliability of experimental results, and providing strong support for the application of biological manufacturing technology in the field of cell culture and tissue engineering. BRIEF DESCRIPTION OF DRAWINGS

[0038] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0039] Figure 1 The attached figure is a schematic diagram of the structure of the fully automated electrospinning membrane processing and cell culture partitioning device provided by the present invention;

[0040] Figure 2 The attached figure is a structural schematic diagram of the Z-axis lifting seat provided by the present invention;

[0041] Figure 3 The attached figure is provided by the present invention. Figure 2 A schematic diagram of the chip section after removing the Z-axis lifting seat;

[0042] Figure 4 The attached figure is provided by the present invention. Figure 3 A schematic diagram of the structure after removing the clamping mechanism;

[0043] Figure 5 The attached figure is a schematic diagram of the clamping plate provided by the present invention;

[0044] Figure 6 The attached figure is provided by the present invention. Figure 4 A schematic diagram of the structure after the clamping components are removed;

[0045] Figure 7 The attached figure is provided by the present invention. Figure 6 A schematic diagram of the structure after removing the telescopic rod portion;

[0046] Figure 8 The attached figure is a schematic diagram of the structure of the telescopic rod portion provided by the present invention.

[0047] Figure 9 The attached figure is a structural schematic diagram of the clamping component provided by the present invention;

[0048] Figure 10 The attached figure is a schematic diagram of the electrospinning mechanism provided by the present invention;

[0049] Figure 11 The attached figure is a schematic diagram of the X-axis portion of the electrospinning mechanism provided by the present invention;

[0050] Figure 12 The attached figure is a schematic diagram of the Y-axis portion of the electrospinning mechanism provided by the present invention;

[0051] Figure 13The drawing is a schematic diagram of the first step of the operation process of the full-automatic electrospinning membrane processing and cell culture partition device provided by the application.

[0052] Figure 14 The drawing is a schematic diagram of the second step of the operation process of the full-automatic electrospinning membrane processing and cell culture partition device provided by the application.

[0053] Figure 15 The drawing is a schematic diagram of the third step of the operation process of the full-automatic electrospinning membrane processing and cell culture partition device provided by the application.

[0054] Figure 16 The drawing is a schematic diagram of the fourth step of the operation process of the full-automatic electrospinning membrane processing and cell culture partition device provided by the application.

[0055] Figure 17 The drawing is a schematic diagram of the fifth step of the operation process of the full-automatic electrospinning membrane processing and cell culture partition device provided by the application.

[0056] Figure 18 The drawing is a schematic diagram of the sixth step of the operation process of the full-automatic electrospinning membrane processing and cell culture partition device provided by the application.

[0057] Figure 19 The drawing is a schematic diagram of the seventh step of the operation process of the full-automatic electrospinning membrane processing and cell culture partition device provided by the application.

[0058] Figure 20 The drawing is a schematic diagram of the eighth step of the operation process of the full-automatic electrospinning membrane processing and cell culture partition device provided by the application.

[0059] Figure 21 The drawing is a schematic diagram of the ninth step of the operation process of the full-automatic electrospinning membrane processing and cell culture partition device provided by the application.

[0060] Figure 22 The drawing is a schematic diagram of the tenth step of the operation process of the full-automatic electrospinning membrane processing and cell culture partition device provided by the application.

[0061] Wherein:

[0062] 1-Z-axis lifting seat;

[0063] 2-chip;

[0064] 21-printing groove; 211-second convex edge; 22-mounting groove; 221-first convex edge; 222-sliding groove; 23-sealing groove; 24-step edge;

[0065] 3-supporting mechanism;

[0066] 31 - telescopic rod; 32 - sliding seat; 321 - sliding block; 33 - electromagnet mounting frame; 34 - electromagnet; 35 - magnet;

[0067] 4 - electrospinning mechanism;

[0068] 41 - spinning receiving frame; 42 - X-axis sliding rail; 43 - X-axis moving seat; 44 - X-axis lead screw; 45 - X-axis drive motor; 46 - Y-axis sliding rail; 47 - Y-axis sliding block; 471 - threaded seat; 48 - Y-axis drive motor; 49 - Y-axis lead screw;

[0069] 5 - clamping mechanism;

[0070] 51 - clamping plate; 511 - fourth protruding edge; 52 - motor fixing frame; 53 - stepping motor;

[0071] 6 - pressing member;

[0072] 61 - third protruding edge;

[0073] 7 - sealing strip. DETAILED DESCRIPTION

[0074] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0075] Referring to the drawings Figure 1 to the drawings Figure 10 The embodiments of the present application disclose a full-automatic electrospinning membrane processing and cell culture partition device, which comprises:

[0076] a Z-axis lifting seat 1;

[0077] a chip 2, which is detachably connected to the Z-axis lifting seat 1, and has a printing pit 21 on the top surface of the chip 2, the bottom surface of the printing pit 21 being used for printing structural layer material;

[0078] a supporting mechanism 3, which is installed on the chip 2 and located on one side of the printing pit 21, and controls the telescopic rod 31 to extend when the printing of the structural layer material on the bottom surface of the printing pit 21 is completed, the telescopic rod 31 being located above the structural layer material;

[0079] an electrospinning mechanism 4, which is arranged outside the Z-axis lifting seat 1 and has a movable spinning receiving frame 41, and when the telescopic rod 31 extends, the spinning receiving frame moves to above the printing pit 21 and covers the membrane on the spinning receiving frame 41 on the upper surface of the telescopic rod 31;

[0080] Clamping mechanism 5, clamping mechanism 5 can be detachable connection in the two sides of the chip 2, when the film is completed cover, clamping plate 51 of clamping mechanism 5 is folded and pressed on the outside of the printing pit 21, and cooperates with the telescopic rod 31 to press the film, and finally prints the functional material layer on the top surface of the film; after the above operation, the chip 2 is detached from the Z axis lifting seat 1, and the subsequent culture medium is injected for co-culture.

[0081] Referring to the accompanying drawings Figure 6 and the accompanying drawings Figure 7 , the top surface of the chip 2 has a mounting pit 22 located on one side of the printing pit 21, a plurality of parallel arranged telescopic rods 31 are slidably arranged in the mounting pit 22, and a sliding seat 32 is slidably connected to the bottom surface of the mounting pit 22. The sliding seat 32 has a plurality of semicircular grooves embedded with the end of the plurality of telescopic rods 31. When the telescopic rod 31 is not extended, the sliding seat 32 is located on the side of the mounting pit 22 away from the first convex edge 221, and the two ends of the telescopic rod 31 are embedded with the semicircular grooves on the sliding seat 32 and the first convex edge 221 respectively. The side of the printing pit 21 away from the mounting pit 22 has a second convex edge 211, and the second convex edge 211 has a plurality of semicircular grooves capable of being embedded with the plurality of telescopic rods 31. When the telescopic rod 31 is extended, the sliding seat 32 moves towards the first convex edge 221 and abuts against the first convex edge 221, and the end of the telescopic rod 31 away from the sliding seat 32 is embedded with the semicircular groove on the second convex edge 211. The telescopic rod 31 has a gap with the bottom surface of the printing pit 21 for printing of the structural layer material.

[0082] Referring to the accompanying drawings Figure 9 , it also includes a pressing member 6 buckled on the top surface of the mounting pit 22, the pressing member 6 is detachably connected with the top surface of the chip 2, the bottom surface of the pressing member 6 has a third convex edge 61 corresponding to the first convex edge 221, the third convex edge 61 has a semicircular groove embedded with the telescopic rod 31, and the semicircular groove on the first convex edge 221 and the semicircular groove on the third convex edge 61 combine to form a circular hole for the telescopic rod 31 to pass through.

[0083] Referring to the accompanying drawings Figure 7 and the accompanying drawings Figure 8 , the bottom surface of the mounting pit 22 has a sliding groove 222 on both sides, and the sliding seat 32 has a sliding block 321 slidably matched with the sliding groove 222.

[0084] Referring to the accompanying drawings Figure 6The Z-axis lifting seat 1 is detachably connected with an electromagnet mounting rack 33, the electromagnet mounting rack 33 is located at the outer side of the chip 2 close to the installation pit 22, the electromagnet mounting rack 33 is provided with an electromagnet 34, and the sliding seat 32 is fixed with a magnet 35; when the electromagnet 34 is powered on, the sliding seat 32 is driven to move through the repulsion force of the magnet 35, and then the telescopic rod 31 is controlled to extend. After the electromagnet 34 is turned on, the repulsion force generated by the magnet drives the telescopic rod 31 to extend, thereby achieving the supporting effect, and simultaneously serving as a channel to simulate the environment in the body.

[0085] Referring to the accompanying drawings Figure 3 to the accompanying drawings Figure 5 The clamping mechanism 5 comprises two motor fixing racks 52 which are detachably connected on both sides of the chip 2, the two motor fixing racks 52 are fixedly provided with a stepping motor 53, the power output shafts of the two stepping motors 53 are fixedly connected with a clamping plate 51, the clamping plate 51 is provided with a fourth convex edge 511 on both sides of the end, and the fourth convex edge 511 is provided with a semicircular groove for embedding the telescopic rod 31; when the clamping plate 51 is folded and pressed, the semicircular groove on the fourth convex edge 511 is embedded with the telescopic rod 31.

[0086] In order to further optimize the above technical scheme, the both sides of the printing pit 21 are provided with a through sealing pit 23, the clamping plate 51 is embedded in the sealing pit 23 after being folded and pressed, and the sealing strip 7 is embedded between the side wall and the bottom surface of the sealing pit 23 and the side wall and the bottom surface of the clamping plate 51.

[0087] Referring to the accompanying drawings Figure 10 and the accompanying drawings Figure 11 The electrospinning mechanism 4 comprises an X-axis sliding rail 42, the X-axis sliding rail 42 is slidably connected with an X-axis moving seat 43, the X-axis sliding rail 42 is rotatably connected with an X-axis lead screw 44, the X-axis lead screw 44 penetrates through the X-axis moving seat 43 and is threadedly connected with the X-axis moving seat 43, one end of the X-axis sliding rail 42 is fixedly provided with an X-axis driving motor 45, and the power output shaft of the X-axis driving motor 45 is fixedly connected with one end of the X-axis lead screw 44.

[0088] Referring to the accompanying drawings Figure 12 The X-axis moving seat 43 is fixedly provided with a Y-axis sliding way 46, the Y-axis sliding way 46 is slidably connected with a Y-axis sliding block 47, one end of the Y-axis sliding block 47 is fixedly connected with the spinning receiving rack 41, the other end is fixedly provided with a threaded seat 471, the X-axis moving seat 43 is fixedly provided with a Y-axis driving motor 48, the power output shaft of the Y-axis driving motor 48 is fixedly connected with a Y-axis lead screw 49, and the Y-axis lead screw 49 is threadedly connected with the threaded seat 471.

[0089] In order to further optimize the above technical scheme, the spinning receiving frame 41 is a U-shaped frame body, the film is formed in the U-shaped opening of the spinning receiving frame 41, the chip 2 has a stepped edge 24 on the two sides of the outer portion, when the film is covered, the U-shaped opening of the spinning receiving frame 41 spans the two sides of the printing pit 21, and the two side supporting rods of the spinning receiving frame 41 abut against the stepped edges 24 on the two sides.

[0090] The working principle of the full-automatic electrospinning film processing and cell culture partition device provided by the application is as follows:

[0091] Firstly, each component of the whole device is in an initial position, as shown in the figure, wherein the X-axis moving seat 43 is located at the rightmost end of the X-axis sliding rail 42, the magnet 35 and the telescopic rod 31 are located inside the pressing member 6, and the clamping plate 51 is driven to be opened to the two sides by the stepping motor 53. Figure 13

[0092] Next, the electrospinning process is performed, and the spinning receiving frame 41 is responsible for receiving the spinning material. After the spinning is completed, the bio-ink is first extruded on the bottom surface of the printing pit 21 through the extrusion printing process. Subsequently, the remote control electromagnet 34 is opened and an repulsive force is applied to push the telescopic rod to be completely pushed out, as shown in the figure, to prepare for the subsequent support and channel. Figure 14

[0093] Then, the Z-axis lifting seat 1 where the chip 2 is located is lowered by a distance, in this embodiment, the Z-axis lifting seat 1 is also controlled to be lifted and lowered through a screw sliding block structure, which will not be described herein. And the spinning receiving frame 41 is moved to a specified position, as shown in the figure. The Z-axis is moderately lifted again, so that the spun film is in contact with the upper side of the telescopic rod 31, and then the stepping motor 53 is driven to close the clamping plate 51, so that the spun film is pressed below the clamping plate 51. Finally, another bio-ink is extruded on the upper side of the film. Figure 17

[0094] The application discloses a full-automatic electrospinning film processing and cell culture partition device, compared with a traditional biological manufacturing device, the application can automatically and accurately displace an electrospinning film to a chip position, sealing and clamping are realized on both sides of the film by driving a stepping motor, so that partition culture between upper and lower chambers is ensured, and integrated molding is realized.

[0095] Specifically, the specific steps of the embodiment are as follows:

[0096] Firstly, the equipment is checked, the power supply is turned on, and it is determined that each component on the device is in an initial position; at this time, the X-axis moving seat 43 is located at the rightmost end of the X-axis sliding rail 42, the magnet 35 and the telescopic rod 31 are located inside the pressing member 6, and the clamping plate 51 is driven to be opened to the two sides by the stepping motor 53. First, the electrospinning process is performed, and the material is spun on the spinning receiving frame 41, and the required time is about 2-3 hours, as shown in the figure. Figure 13 ​​​As shown.

[0097] Second step, after the electrospinning process is completed, the extrusion printing process is first carried out, and the biomaterial is printed to the bottom surface of the printing pit 21. After printing is completed, the electromagnet 34 is opened, and the repulsive force generated drives the magnet 35 to push the telescopic rod 31 out, as shown in Figure 14 As shown.

[0098] Third step, control the Y-axis drive motor 48 to retract the spinning receiving frame 41, as shown in Figure 15 As shown.

[0099] Fourth step, let the Z-axis lifting seat 1 where the chip 2 is located move down by a distance, and move the X-axis moving seat 43 to the designated position, as shown in Figure 16 As shown.

[0100] Fifth step, control the Y-axis drive motor 48 to push the spinning receiving frame 41 out to the designated position, as shown in Figure 17 As shown.

[0101] Sixth step, after pushing out, then the Z-axis lifting seat 1 is moved up again, so that the spun film on the spinning receiving frame 41 is in contact with the telescopic rod 31 above, as shown in Figure 18 As shown.

[0102] Seventh step, drive the stepping motor 53 to close the clamping plates 51 on the left and right sides. After clamping, the microbeads are printed into the pits on the upper layer of the film in the form of microbead printing, as shown in Figure 19 As shown.

[0103] Eighth step, control the Y-axis drive motor 48 to retract the spinning receiving frame 41, as shown in Figure 20 As shown.

[0104] Ninth step, reset the X-axis moving seat 43 to the rightmost end of the X-axis sliding rail 42, as shown in Figure 21 As shown.

[0105] Tenth step, after printing is completed, take down the chip 2 from the Z-axis lifting seat 1, as shown in Figure 22 As shown, the culture medium can be injected for co-culture after that. After the operation is completed, the power is turned off.

[0106] In this embodiment, the Z-axis lifting seat 1 is uniformly distributed with a plurality of mounting holes, and the chip 2, the electromagnet mounting frame 33 and the motor fixing frame 52 and other structures have corresponding holes, and the holes are used for corresponding connection and fixation through connecting pieces.

[0107] The various embodiments described in this specification are implemented in a progressive manner, each embodiment focusing on the differences from other embodiments, and the same or similar parts between embodiments can be mutually referred to. For the apparatus disclosed by the embodiments, since it corresponds to the method disclosed by the embodiments, the description is relatively simple, and the relevant parts can be referred to the description of the method part.

[0108] The above description of disclosed embodiments enables a person skilled in the art to implement or use the present application. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present application. Therefore, the present application will not be limited to these embodiments shown herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A fully automated electrospun membrane processing and cell culture partitioning device, characterized in that, include: Z-axis lifting seat (1); Chip (2), the chip (2) is detachably connected to the Z-axis lifting seat (1), the top surface of the chip (2) has a printing pit (21), and the bottom surface of the printing pit (21) is used to print structural layer material; Support mechanism (3) is mounted on the chip (2) and located on one side of the printing pit (21). After the structural layer material is printed on the bottom surface of the printing pit (21), the support mechanism (3) controls its telescopic rod (31) to extend. The telescopic rod (31) is located above the structural layer material. An electrospinning mechanism (4) is located on the outside of the Z-axis lifting seat (1), and the electrospinning mechanism (4) has a movable spinning receiving frame (41). When the telescopic rod (31) extends, the spinning receiving frame moves to the top of the printing pit (21) and covers the upper surface of the telescopic rod (31) with the film on the spinning receiving frame (41). The clamping mechanism (5) is detachably connected to both sides of the chip (2). After the film is covered, the clamping plate (51) of the clamping mechanism (5) is folded and pressed against the outside of the printing pit (21), and cooperates with the telescopic rod (31) to press the film. Finally, the functional material layer is printed on the top surface of the film. After the above operations are completed, the chip (2) is removed from the Z-axis lifting seat (1), and the culture medium is injected for co-culture.

2. The fully automated electrospinning membrane processing and cell culture partitioning device according to claim 1, characterized in that, The chip (2) has a mounting groove (22) on its top surface located on one side of the printing groove (21). Multiple parallel telescopic rods (31) are slidably arranged within the mounting groove (22). A sliding seat (32) is slidably connected to the bottom surface of the mounting groove (22). The sliding seat (32) has multiple semi-circular grooves that engage with the ends of the telescopic rods (31). The mounting groove (22) has a first protruding edge (221) near the edge of the printing groove (21). The first protruding edge (221) has multiple semi-circular grooves that engage with the telescopic rods (31). When the telescopic rods (31) are not extended, the sliding seat (32) is located on the side of the mounting groove (22) away from the first protruding edge (221). The two ends of the telescopic rod (31) are respectively fitted into the semi-circular grooves on the sliding seat (32) and the first protrusion (221); the printing pit (21) has a second protrusion (211) on the side away from the mounting pit (22), and the second protrusion (211) has a plurality of semi-circular grooves that can be fitted into multiple telescopic rods (31). When the telescopic rod (31) extends, the sliding seat (32) moves toward the first protrusion (221) and abuts against the first protrusion (221). The end of the telescopic rod (31) away from the sliding seat (32) is fitted into the semi-circular groove on the second protrusion (211). The telescopic rod (31) and the bottom surface of the printing pit (21) have a gap for printing the structural layer material.

3. The fully automated electrospinning membrane processing and cell culture partitioning device according to claim 2, characterized in that, It also includes a clamping member (6) fastened to the top surface of the mounting groove (22). The clamping member (6) is detachably connected to the top surface of the chip (2). The bottom surface of the clamping member (6) has a third protrusion (61) corresponding to the first protrusion (221). The third protrusion (61) has a semi-circular groove that fits into the telescopic rod (31). The semi-circular groove on the first protrusion (221) and the semi-circular groove on the third protrusion (61) combine to form a circular hole through which the telescopic rod (31) passes.

4. The fully automated electrospinning membrane processing and cell culture partitioning device according to claim 2, characterized in that, The bottom surface of the mounting pit (22) has sliding grooves (222) on both sides, and the sliding seat (32) has sliders (321) on both sides that slide in cooperation with the sliding grooves (222).

5. The fully automated electrospinning membrane processing and cell culture partitioning device according to claim 2, characterized in that, An electromagnet mounting bracket (33) is detachably connected to the Z-axis lifting seat (1). The electromagnet mounting bracket (33) is located on the outside of the chip (2) near the mounting slot (22). An electromagnet (34) is mounted on the electromagnet mounting bracket (33). A magnet (35) is fixed on the sliding seat (32). When the electromagnet (34) is energized, it drives the sliding seat (32) to move by the repulsive force with the magnet (35), thereby controlling the extension of the telescopic rod (31).

6. The fully automated electrospinning membrane processing and cell culture partitioning device according to claim 2, characterized in that, The clamping mechanism (5) includes two motor mounting brackets (52) detachably connected to both sides of the chip (2). Each of the two motor mounting brackets (52) is fixedly installed with a stepper motor (53). The clamping plate (51) is fixedly connected to the power output shaft of each of the two stepper motors (53). The clamping plate (51) has a fourth protrusion (511) on both sides of its end. The fourth protrusion (511) has a semi-circular groove for engaging with the telescopic rod (31). When the clamping plate (51) is folded and pressed against the film, the semi-circular groove on the fourth protrusion (511) engages with the telescopic rod (31).

7. The fully automated electrospinning membrane processing and cell culture partitioning device according to claim 6, characterized in that, The printing groove (21) has through sealing grooves (23) on both sides. The clamping plate (51) is folded and pressed and then embedded in the sealing groove (23). A sealing strip (7) is embedded between the side wall and bottom surface of the sealing groove (23) and the side wall and bottom surface of the clamping plate (51).

8. The fully automated electrospinning membrane processing and cell culture partitioning device according to claim 1, characterized in that, The electrospinning mechanism (4) includes an X-axis slide rail (42), an X-axis moving seat (43) is slidably connected to the X-axis slide rail (42), an X-axis lead screw (44) is rotatably connected to the X-axis slide rail (42), the X-axis lead screw (44) passes through the X-axis moving seat (43) and is threadedly connected to the X-axis moving seat (43), an X-axis drive motor (45) is fixed at one end of the X-axis slide rail (42), and the power output shaft of the X-axis drive motor (45) is fixedly connected to one end of the X-axis lead screw (44).

9. The fully automated electrospinning membrane processing and cell culture partitioning device according to claim 8, characterized in that, A Y-axis slide rail (46) is fixed on the X-axis moving seat (43), and a Y-axis slider (47) is slidably connected on the Y-axis slide rail (46). One end of the Y-axis slider (47) is fixed to the spinning receiving frame (41), and the other end is fixed to a threaded seat (471). A Y-axis drive motor (48) is fixed on the X-axis moving seat (43), and a Y-axis lead screw (49) is fixedly connected to the power output shaft of the Y-axis drive motor (48). The Y-axis lead screw (49) is threadedly connected to the threaded seat (471).

10. The fully automated electrospinning membrane processing and cell culture partitioning device according to claim 1, characterized in that, The spinning receiver (41) is a U-shaped frame. The film is formed in the U-shaped opening of the spinning receiver (41). The chip (2) has stepped edges (24) on both sides of its exterior. When the film is covered, the U-shaped opening of the spinning receiver (41) spans both sides of the printing pit (21), and the two side supports of the spinning receiver (41) abut against the stepped edges (24) on both sides.

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