Full-automatic anti-acid gram reading and analyzing equipment

Abstract

The invention relates to full-automatic anti-acid gram reading and analyzing equipment which comprises a rack, a workbench is arranged on one side of the rack, and an automatic feeding and discharging device and a dyeing treatment device are arranged on the workbench; a first conveying mechanism and a second conveying mechanism are arranged on the rack, the first conveying mechanism is used for conveying glass slides on the automatic feeding and discharging device to the dyeing treatment device, and the second conveying mechanism is used for conveying the glass slides between the dyeing treatment device and the visual judgment device; the automatic feeding and discharging device comprises a material carrying box placed on the workbench, a plurality of clamping grooves are formed in the material carrying box, and the bottom of the workbench is provided with a feeding assembly for pushing the material carrying box to automatically slide to the position below the first conveying mechanism, a material ejecting assembly and a discharging assembly. The dyeing treatment device comprises a feeding box, and a coloring agent adding mechanism, a cleaning mechanism and an air drying mechanism are arranged on one side of the feeding box. The method has the effects of replacing traditional complete dependence on manual operation and improving the detection efficiency.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of microorganism detection, and in particular to a full-automatic acid-fast gram reading film analysis device. BACKGROUND

[0002] In the field of microorganism detection, acid-fast staining and gram staining are two most commonly used bacteriological staining techniques. Acid-fast staining is mainly used for detection of acid-fast bacteria such as Mycobacterium tuberculosis, and is mainly used for detection of acid-fast bacteria (their cell walls contain special lipids and can resist acid decolorizing agents, showing red color) such as Mycobacterium tuberculosis, which is the "gold standard" for diagnosis of Mycobacterium infection such as tuberculosis; gram staining is used to distinguish gram-positive bacteria and gram-negative bacteria, and quickly distinguishes bacterial types through the difference in affinity of bacterial cell walls to dyes (gram-positive bacteria show purple color, and gram-negative bacteria show red color), combined with morphological characteristics (such as cocci and bacilli), to provide a key basis for early medication for infectious diseases.

[0003] The traditional staining process completely relies on manual operation, including sample smearing, fixing, staining, decolorizing, re-staining, rinsing, and drying, and the whole process takes about 30-45 minutes. With the increase of the amount of detection samples, the efficiency bottleneck of manual operation is increasingly prominent, and there are problems such as low standardization of operation and strong subjectivity of result interpretation. In recent years, some laboratories have begun to try to introduce semi-automatic equipment, but most of them can only complete a single staining function and cannot realize full-process automation from sample processing to result analysis. SUMMARY

[0004] In order to replace the traditional completely manual operation and improve the detection efficiency, the present application provides a full-automatic acid-fast gram reading film analysis device.

[0005] The full-automatic acid-fast gram reading film analysis device provided by the present application adopts the following technical solution: A full-automatic acid-fast gram reading film analysis device, comprising a rack, a horizontal workbench is arranged on one side of the rack, an automatic feeding and discharging device and a staining treatment device are sequentially arranged on the workbench, and a visual judgment device is arranged at one end of the rack and the workbench; a horizontal rack is arranged on the rack, a first transmission mechanism and a second transmission mechanism are arranged on the rack, the first transmission mechanism is used for transmitting a slide on the automatic feeding and discharging device to the staining treatment device, and the second transmission mechanism is used for transmitting the slide between the staining treatment device and the visual judgment device; the automatic feeding and discharging device comprises a loading box placed on the workbench, a plurality of clamping grooves are formed in the loading box, the clamping grooves are used for inserting and connecting the slide, and a feeding assembly, a lifting assembly and a discharging assembly for automatically sliding the loading box to below the first transmission mechanism are arranged at the bottom of the workbench; the staining treatment device comprises a feeding box, a staining agent adding mechanism, a cleaning mechanism and an air drying mechanism are arranged on one side of the feeding box.

[0006] By adopting the above technical solution, when using the machine, the user places the material box containing the slides on the worktable. The feeding component pushes the material box to the bottom of the frame, and the top component pushes the material box to the bottom of the first transmission mechanism for easy retrieval. The discharge component pushes the tested material box outward, completing automatic feeding and automatic discharge. The first transmission mechanism transports the slides to the feeding box, and the staining agent adding mechanism automatically adds staining agent to the slides. The second transmission mechanism transports the slides between the staining agent adding mechanism, the cleaning mechanism, and the drying mechanism. Finally, the second transmission mechanism transports the stained slides to the visualization judgment device. The visualization judgment device uses the magnification function of a microscope to intuitively identify and judge the presence, morphology, and distribution characteristics of acid-fast bacteria, thereby automatically completing the automatic feeding, staining, transmission, and identification of slides, replacing the traditional method that relies entirely on manual operation and improving detection efficiency.

[0007] Optionally, the workbench is divided into a feeding area and a discharging area. The feeding area is located near the dyeing device, and the discharging area is located away from the dyeing device. Both the feeding and discharging components include a pusher motor fixedly connected to the bottom of the workbench. The output shaft of the pusher motor is fixedly connected to a sliding block. A feeding screw is rotatably connected to the bottom of the workbench. The sliding block is threaded onto the feeding screw. Pushing rods rotatably rotate on both sides of the sliding block. The top of each pushing rod has an inclined surface, which slopes upwards towards the direction of transmission. A clearance groove is provided at the position of the feeding rod, and the feeding rod extends into the reversing groove. A stop bar is fixedly connected to the sliding block, and the stop bar is used to limit the feeding rod. A connecting channel is provided between the feeding area and the unloading area. The top material assembly includes a guide rail fixedly connected to the frame. The guide rail is located above the connecting channel. A top material block is slidably connected to the guide rail. A top material motor is provided on the frame. A push screw is fixedly connected to the output shaft of the top material motor. The axial direction of the push screw is perpendicular to the axial direction of the feeding screw. The top material block is threadedly connected to the threaded column.

[0008] By adopting the above technical solution, when the user uses the device, the material box containing the glass slide is placed in the feeding area. The pusher motor in the feeding area works, driving the sliding block to slide. The pusher rod on the sliding block slides the material box towards the connecting channel. The inclined surface at the top of the pusher rod facilitates avoidance with the material box when the pusher rod slides in the opposite direction. The stop bar limits the range of rotation of the pusher rod, enabling the pusher rod to feed in one direction. The top motor works, driving the top block to push the material box from the feeding area to the unloading area, realizing the automatic feeding of the material box and the glass slide.

[0009] Optionally, both the first and second feeding mechanisms include a sliding frame slidably connected to the frame, a drive motor fixedly connected to the sliding frame, and a gear fixedly connected to the output shaft of the drive motor, with the gear meshing with a rack; a power motor is rotatably connected to the top of the sliding frame, and a feeding screw is fixedly connected to the output shaft of the power motor, the feeding screw is vertically oriented, a picking frame is threadedly connected to the feeding screw, a tilting motor is fixedly connected to one side of the picking frame, the output shaft of the tilting motor extends into the picking frame and is fixedly connected to a clamping motor, and an electromagnetic chuck is fixedly connected to the output shaft of the clamping motor.

[0010] By adopting the above technical solution, when the user uses the device, firstly, the power motor drives the feeding screw to rotate, causing the material picker to slide vertically. When the feeding rack slides down to the bottom, the electromagnetic claw on the clamping motor grabs the glass slide in the material box. The flipping motor drives the clamping motor and the electromagnetic claw to flip, which can change the angle of the glass slide. Finally, the drive motor drives the gear to rotate, and the gear moves along the rack, which can transfer the glass slide to the dyeing treatment device, completing the automatic feeding and material handling.

[0011] Optionally, the top of the feeding box has a feed inlet for convenient insertion of glass slides into the feeding box; the staining agent adding mechanism includes multiple reagent kits fixedly connected to one side of the workbench, each reagent kit containing various staining agents; a base plate is fixedly connected to the workbench, a stepper motor is fixedly connected to one side of the base plate, a first screw is fixedly connected to the output shaft of the stepper motor, a guide block is threaded onto the first screw, a feeding plate is fixedly connected to the guide block, multiple drop pumps are fixedly connected to the feeding plate, each drop pump is equipped with a dropper, and each drop pump is fixedly connected to a feeding tube, which communicates with different reagent kits respectively; the side wall of the feeding box has a feeding port for the feeding plate to enter and exit.

[0012] By adopting the above technical solution, when the user uses the product, the slide is tilted and inserted into the feeding box. The first motor drives the first screw to slide, which in turn drives the feeding plate to slide into the feeding box. The drip pump adds the dye to the slide through the dropper, thus completing the automatic feeding of the dye.

[0013] Optionally, the feeding box is equipped with a spray head, and a water outlet pipe is provided on one side of the feeding box, with a pump connected to the water outlet pipe.

[0014] By adopting the above technical solution, when users use the product, cleaning is a key operation that runs through multiple steps in the acid-fast detection and Gram detection process. Its core purpose is to remove excess reagents, avoid interference, and ensure the accuracy and clarity of the staining results.

[0015] Optionally, a support is provided on one side of the base plate, a conveyor belt is provided on the support, unused droppers are placed on the conveyor belt, and a motor is provided on the support to drive the conveyor belt to slide; a second motor is fixedly connected to the side wall of the feeding box, a swing arm is fixedly connected to the output shaft of the second motor, a rotating shaft is hinged to the end of the swing arm, a third motor is fixedly connected to the swing arm, the output shaft of the third motor is fixed to the rotating shaft, and a positioning box is fixedly connected to the rotating shaft; a clamping seat is slidably connected inside the positioning box, and an insertion sleeve is fixedly connected to the end of the clamping seat for inserting the dropper; electromagnetic clamping rods are provided on both sides of the clamping seat, a reset cavity is opened inside the clamping seat, a reset spring is fixedly connected inside the reset cavity, one end of the electromagnetic clamping rod is fixedly connected to the reset spring, and the other end of the electromagnetic clamping rod is used to clamp the end of the dropper; a power component for pushing the dropper to be inserted into the drip pump is provided inside the clamping seat.

[0016] By adopting the above technical solution, when the user uses the device, the second motor drives the swing arm to rotate above the conveyor belt, and the third motor drives the rotating shaft to rotate, which in turn moves the clamping seat and the electromagnetic clamping rod to the top of the conveyor belt. The tapered part of the dropper is inserted into the insertion cylinder, and the electromagnetic clamping rod clamps the dropper, thus fixing the dropper onto the electromagnetic clamping rod. The second motor drives the swing arm to move in the opposite direction to the side of the feeding plate, and the first motor drives the first screw to slide, causing the feeding plate to slide out of the feeding box, so that the position of the dropper corresponds to that of the drip pump. Finally, the feeding assembly pushes the clamping seat to slide towards the feeding plate, so that the dropper is automatically inserted into the drip pump, completing the automatic installation of the dropper. After the electromagnetic clamping rod is de-energized, the tension of the return spring separates the electromagnetic clamping rod from the dropper, completing the automatic installation of the dropper.

[0017] Optionally, the power assembly includes a fourth motor fixed inside the positioning box. The fourth motor is located on the side of the clamping seat away from the insertion cylinder. A threaded column is fixedly connected to the output shaft of the fourth motor, and the clamping seat is threadedly connected to the threaded column.

[0018] By adopting the above technical solution, when the dropper is aligned with the position of the drip pump, the fourth motor is powered on, driving the clamping seat to slide towards the upward material plate, so that the dropper is automatically inserted into the drip pump, completing the automatic installation of the dropper.

[0019] Optionally, the drying assembly includes a drying chamber with an insertion interface on the top and a blower on one side of the drying chamber.

[0020] By adopting the above technical solution, when users use the acid-fast staining and Glax staining steps, they need to rinse with running water after each staining to remove excess staining solution. After rinsing, there will be residual moisture on the smear. If it is air-dried naturally, it will take a long time. However, subsequent steps need to be carried out immediately. The fan drying can quickly remove moisture through airflow, shorten the drying time, ensure that each staining step is connected in an orderly manner, avoid the residual moisture in the previous step from diluting the subsequent staining agent, ensure the stability of the staining agent concentration, and reduce the deviation of staining effect caused by excessive time.

[0021] Optionally, the worktable is equipped with an immersion box, which has multiple reagent slots for inserting glass slides. A water pump is installed on one side of the reagent slots, and a feed pipe is connected to the water pump and connected to the immersion solution. A slide rail is fixedly connected to the worktable, and a fifth motor is fixedly connected to one side of the slide rail. A positioning block is fixedly connected to the output shaft of the fifth motor, and the feed pipe is fixedly connected to the positioning block. The end of the feed pipe extends above the reagent slots.

[0022] By adopting the above technical solution, when the user uses the water pump, it can guide the soaking solution to the feeding pipe, and then guide it to the reagent tank through the feeding pipe. The fifth motor works to push the positioning block to slide and add materials to different reagent tanks.

[0023] Optionally, the visualization judgment device includes a frame, a stage on the frame, and a microscope positioned above the stage on the frame.

[0024] By adopting the above technical solution, when the user uses the device, the second transmission mechanism places the slide on the stage and magnifies the fine structure and staining characteristics of the bacteria through a microscope, which helps researchers or clinical laboratory personnel to observe, identify and distinguish different types of bacteria. Attached Figure Description

[0025] Figure 1 This is a schematic diagram of the overall structure of an embodiment of this application; Figure 2 This is a structural diagram provided in this application to highlight the positional relationship between the automatic loading and unloading device, the dyeing treatment device, the first transmission mechanism, and the second transmission mechanism; Figure 3 This is a schematic diagram of the automatic loading and unloading device; Figure 4 This is a structural diagram designed to highlight the positional relationship between the feeding and discharging components; Figure 5 This is a cross-sectional view of an automatic loading and unloading device; Figure 6 This is a structural diagram of the frame, the first transmission mechanism, and the second transmission mechanism; Figure 7 This is a schematic diagram of the structure of the first transmission mechanism and the rack; Figure 8 This is a schematic diagram of the dyeing treatment device; Figure 9 This is an exploded view of the feeding box and dye addition mechanism; Figure 10 This is a schematic diagram of the dye addition mechanism; Figure 11 This is a cross-sectional view made to highlight the electromagnetic clamp, dropper, and feeding plate; Figure 12 This is a structural diagram designed to highlight the positional relationship between the visualization judgment device and the frame and workbench.

[0026] Explanation of reference numerals in the attached drawings: 1. Frame; 11. Rack; 12. Protective cover; 2. Worktable; 21. Clearing groove; 3. Automatic loading and unloading device; 31. Material box; 311. Slot; 32. Loading area; 33. Unloading area; 34. Feeding assembly; 341. Pusher motor; 342. Sliding block; 343. Loading screw; 344. Pushing rod; 345. Stop bar; 35. Ejector assembly; 351. Guide rail; 352. Ejector motor; 353. Pusher screw; 35 4. Top material block; 36. Discharge assembly; 4. Dyeing treatment device; 41. Feeding box; 411. Inlet; 412. Loading port; 42. Dyeing agent addition mechanism; 421. Reagent kit; 422. Base plate; 423. Stepper motor; 424. First screw; 425. Guide block; 426. Loading plate; 4261. Dropping pump; 4262. Dropper; 427. Loading tube; 43. Support; 431. Conveyor belt; 432. Second motor; 433. Swing arm; 434. Rotating shaft; 435. Third motor; 436. Positioning box; 4361. Clamping seat; 4362. Insert sleeve; 4363. Electromagnetic clamping rod; 4364. Reset chamber; 4364. Reset spring; 4365. Fourth motor; 4366. Threaded post; 44. Drying mechanism; 441. Drying oven; 442. Insertion interface; 443. Blower; 45. Cleaning mechanism; 451. Water outlet pipe; 452. Pump; 46. Soaking box; 461. Reagent 462. Water pump; 463. Feed pipe; 464. Slide rail; 465. Fifth motor; 466. Positioning block; 5. First transmission mechanism; 51. Sliding frame; 52. Drive motor; 53. Gear; 54. Power motor; 541. Feeding screw; 542. Picking rack; 55. Tilting motor; 56. Clamping motor; 561. Electromagnetic claw; 6. Second transmission mechanism; 7. Visual judgment device; 71. Carrier frame; 72. Stage; 73. Microscope. Detailed Implementation

[0027] The following is in conjunction with the appendix Figures 1-12 This application will be described in further detail.

[0028] In this application, unless otherwise expressly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, an electrical connection, or a communication connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0029] In this application, unless otherwise expressly specified and limited, the "above" or "below" of the second feature can mean that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediate medium. In the description of this specification, references to terms such as "an embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described can be combined in any suitable manner in one or more embodiments or examples.

[0030] This application discloses a fully automated acid-resistant Gram slide reading and analysis device, referring to... Figure 1 and Figure 2 The fully automated acid-fast Gram slide analysis device provided in this application includes a frame 1, a workbench 2 on one side of the frame 1, an automatic loading and unloading device 3 and a staining device 4 arranged sequentially on the workbench 2, and a visualization judgment device 7 at one end of the frame 1 and the workbench 2. A first transmission mechanism 5 and a second transmission mechanism 6 are horizontally slidably connected on the frame 1 and mounted on a rack 11. The first transmission mechanism 5 transmits the slides from the automatic loading and unloading device 3 to the staining device 4, and the second transmission mechanism 6 is responsible for transmitting the slides between the staining device 4 and the visualization judgment device 7. This arrangement enables the slides to flow orderly between the devices, realizing full automation from sample loading and staining to analysis, and improving detection efficiency.

[0031] A protective cover 12 is installed outside the frame 1 and the workbench 2. The protective cover 12 isolates the test samples and staining agents from the outside world, which not only reduces the risk of germs to the safety of the experimental personnel, but also prevents cross-contamination between the samples and the environment, and protects the experimental personnel from the harm of chemical reagents.

[0032] Reference Figure 3 , Figure 4 and Figure 5Specifically, the automatic loading and unloading device 3 includes a material carrier box 31 placed on the worktable 2. The material carrier box 31 has multiple slots 311 for inserting glass slides. The material carrier box 31 is generally made of plastic or metal, possessing a certain strength and durability. Its shape is typically cuboid, with the slots 311 evenly distributed inside to ensure stable placement of the glass slides. Replaceable material carrier boxes 31 can be of different sizes or have different numbers of slots 311 to adapt to different testing requirements. The bottom of the worktable 2 is equipped with a feeding assembly 34, a top-loading assembly 35, and a discharge assembly 36.

[0033] The workbench 2 is equipped with a loading area 32 and a unloading area 33. A feeding assembly 34 is located in the loading area 32, and a discharging assembly 36 is located in the unloading area 33. A top-loading assembly 35 is located at the junction of the loading and unloading areas 33, used to push the material box 31 from the loading area 32 to the unloading area 33. The feeding assembly 34 and the discharging assembly 36 have the same structure, only their transmission directions are opposite; here, only the feeding assembly 34 is used as an example. The feeding assembly 34 includes a pusher motor 341 fixed to the bottom of the workbench 2. The output shaft of the pusher motor 341 is connected to a sliding block 342. A loading screw 343 is rotatably connected to the bottom of the workbench 2, and the sliding block 342 is threadedly connected to the loading screw 343. The sliding block 342 is rotatably connected to the feeding rod 344 on both sides. The feeding rod 344 has an inclined surface at the top, which slopes upward towards the side facing the conveying direction of the material box 31. The worktable 2 has a relief groove 21 corresponding to the position of the feeding rod 344. The feeding rod 344 extends into the relief groove 21 to push the material box 31. A stop rod 345 is fixedly connected to the sliding block 342. The stop rod 345 is used to limit the rotation range of the feeding rod 344, realizing unidirectional feeding of the material box 31. When the feeding motor 341 is working, it drives the sliding block 342 to slide along the feeding screw 343, and the feeding rod 344 moves accordingly, realizing unidirectional feeding by using the inclined surface.

[0034] The feeding assembly 35 includes a guide rail 351 fixed on the frame 1, a feeding block 354 slidably connected to the guide rail 351, a feeding motor 352 mounted on the frame 1, a pusher screw 353 fixedly connected to the output shaft of the feeding motor 352, the axial direction of the pusher screw 353 being perpendicular to the axial direction of the feeding screw 343, and the feeding block 354 being threadedly connected to the pusher screw 353. The feeding motor 352 drives the feeding block 354 to slide on the guide rail 351, pushing the material box 31 from the feeding area 32 to the unloading area 33. These components cooperate to realize the automatic feeding and automatic unloading of the material box 31 and the glass slides.

[0035] A rack 11 is horizontally arranged on the frame 1. The first transmission mechanism 5 and the second transmission mechanism 6 are both slidably connected to the rack 11. The structures of the first transmission mechanism 5 and the second transmission mechanism 6 are the same. Here, only the first transmission mechanism 5 is taken as an example.

[0036] Reference Figure 6 and Figure 7 Specifically, the first transmission mechanism 5 includes a sliding frame 51 slidably connected to the frame 1, a drive motor 52 fixed on the sliding frame 51, and a gear 53 connected to the output shaft of the drive motor 52. The gear 53 meshes with the rack 11. A power motor 54 is rotatably connected to the top of the sliding frame 51. The output shaft of the power motor 54 is connected to a feeding screw 541. The feeding screw 541 is axially vertically arranged, and a picking frame 542 is threadedly connected to the feeding screw 541. A flipping motor 55 is fixed to one side of the picking frame 542. The output shaft of the flipping motor 55 extends into the picking frame 542 and is connected to a clamping motor 56. The output shaft of the clamping motor 56 is connected to an electromagnetic chuck 561. A power motor 54 drives the feeding screw 541 to rotate, causing the pick-up rack 542 to slide vertically. When the pick-up rack 542 slides to the bottom, the electromagnetic chuck 561 grabs the glass slide. A flip motor 55 can change the angle of the glass slide. The drive motor 52 drives the sliding frame 51 to move through the meshing of the gear 53 and rack 11, thereby realizing the transfer of the glass slide. The drive motor 52 can be a stepper motor 423 or a servo motor to provide precise power control. The accuracy of the gear 53 and rack 11 also affects the accuracy of the transfer; a high-precision gear 53 and rack 11 pair can be selected.

[0037] Reference Figure 8 , Figure 9 and Figure 10 Specifically, the staining treatment device 4 includes a feeding box 41, on one side of which are a staining agent adding mechanism 42, a drying mechanism 44, and a cleaning mechanism 45. The feeding box 41 has a feed inlet 411 at the top for easy insertion of glass slides. The feeding box 41 is generally made of corrosion-resistant materials, such as stainless steel or plastic, to prevent corrosion by the staining agent. The size and shape of the feed inlet 411 are designed according to the size of the glass slides to ensure smooth insertion.

[0038] The staining agent adding mechanism 42 has multiple reagent kits 421 fixed to one side of the workbench 2. Each reagent kit 421 contains different staining agents. A base plate 422 is fixed to the workbench 2, and a stepper motor 423 is fixed to one side of the base plate 422. The output shaft of the stepper motor 423 is connected to a first screw 424. A guide block 425 is threaded onto the first screw 424. A feeding plate 426 is fixed to the guide block 425, and multiple dropper pumps 4261 are fixed to the feeding plate 426. Each dropper pump 4261 is equipped with a dropper 4262, and the dropper pump 4261 is connected to a feeding tube 427, which is connected to different reagent kits 421. The side wall of the feeding box 41 has a feeding port 412 for the feeding plate 426 to enter and exit. The stepper motor 423 drives the first screw 424 to rotate, causing the guide block 425 to move the feeding plate 426. The dropper pumps 4261 add the staining agent to the glass slide through the dropper 4262. The reagent kit 421 can be replaced according to different staining needs, and the drip pump 4261 can also be selected with different flow rates and precision models.

[0039] Reference Figure 9 and Figure 11Furthermore, regarding the installation of the dropper 4262, a bracket 43 is installed on one side of the base plate 422, and a conveyor belt 431 is installed on the bracket 43. Unused droppers 4262 are placed on the conveyor belt 431, and a motor that drives the conveyor belt 431 to slide is located on the bracket 43. A second motor 432 is fixed to the side wall of the feeding box 41. The output shaft of the second motor 432 is connected to a swing arm 433. A rotating shaft 434 is hinged to the end of the swing arm 433. A third motor 435 is fixed to the swing arm 433. The output shaft of the third motor 435 is fixed to the rotating shaft 434, and a positioning box 436 is fixedly connected to the rotating shaft 434. A clamping seat 4361 is slidably connected inside the positioning box 436. A sleeve 4362 is fixedly inserted at the end of the clamping seat 4361 for inserting the dropper 4262. Electromagnetic clamping rods 4363 are symmetrically arranged on both sides of the clamping seat 4361. A reset cavity 4364 is provided inside the clamping seat 4361. A reset spring 4364 is fixed inside the reset cavity 4364. One end of the electromagnetic clamping rod 4363 is connected to the reset spring 4364, and the other end is used to clamp the end of the dropper 4262. A power component is provided inside the clamping seat 4361 to push the dropper 4262 to be inserted into the drip pump 4261. The power component includes a fourth motor 4365 fixed inside the positioning box 436. The output shaft of the fourth motor 4365 is connected to a threaded post 4366. The clamping seat 4361 is threadedly connected to the threaded post 4366. The second motor 432 drives the swing arm 433 to rotate above the conveyor belt 431. The third motor 435 drives the rotating shaft 434 to rotate, so that the clamping seat 4361 and the electromagnetic clamping rod 4363 reach above the conveyor belt 431. The dropper 4262 is inserted into the insertion sleeve 4362. The electromagnetic clamping rod 4363 clamps the dropper 4262. The second motor 432 drives the swing arm 433 to move in the opposite direction to one side of the feeding plate 426. The first motor drives the feeding plate 426 to move so that the dropper 4262 corresponds to the drip pump 4261. The fourth motor 4365 drives the clamping seat 4361 to insert the dropper 4262 into the drip pump 4261. After the electromagnetic clamping rod 4363 is de-energized, the return spring 4364 separates the electromagnetic clamping rod 4363 from the dropper 4262.

[0040] This automatic installation method for the dropper 4262 further enhances the automation level of the equipment. It avoids the hassle and potential errors of manual installation, thus improving work efficiency. Simultaneously, the cooperation of the electromagnetic clamp 4363 and the return spring 4364 ensures reliable clamping and rapid separation of the dropper 4262, guaranteeing the accuracy and stability of its installation.

[0041] Looking back Figure 8 The cleaning mechanism 45 includes a spray head disposed in the feeding box 41 and a water outlet pipe 451 disposed on one side of the feeding box 41, the water outlet pipe 451 being connected to a pump 452. The spray head can spray cleaning fluid evenly to clean the glass slide, while the pump 452 is used to discharge the cleaned liquid.

[0042] The drying mechanism 44 includes a drying chamber 441, with an insertion interface 442 on the top and a blower 443 on one side. The drying chamber 441 is made of heat-insulating material to reduce heat loss, and the blower 443 accelerates airflow to quickly dry the glass slides.

[0043] An immersion box 46 is installed on the workbench 2. The immersion box 46 contains multiple reagent tanks 461 for inserting glass slides. A water pump 462 is installed on one side of each reagent tank 461, connected to a feed pipe 463, which is connected to the immersion solution source. A slide rail 464 is fixedly connected to the workbench 2, and a fifth motor 465 is fixedly connected to one side of the slide rail 464. The output shaft of the fifth motor 465 is connected to a positioning block 466. The feed pipe 463 is fixed to the positioning block 466, and its end extends above the reagent tanks 461. The water pump 462 draws the immersion solution into the reagent tanks 461, and the fifth motor 465 pushes the positioning block 466 to slide, adding solution to different reagent tanks 461. During acid-fast staining experiments, the samples need to be immersed. Glass slides can be inserted into the reagent tanks 461 for immersion treatment, further optimizing the staining effect. By controlling the water pump 462 and the fifth motor 465, soaking solution can be precisely added to different reagent tanks 461 to meet different testing needs. At the same time, the design of the slide rail 464 and the positioning block 466 ensures the accurate movement of the feed pipe 463, improving the accuracy of feeding.

[0044] Reference Figure 12 Specifically, the visualization and judgment device 7 includes a shelf 71, a stage 72 mounted on the shelf 71, and a microscope 73 mounted on the shelf 71 above the stage 72. The stage 72 typically has a smooth, flat design to ensure that the slide is placed stably. The microscope 73 can magnify the fine structure and staining characteristics of bacteria, facilitating observation and judgment.

[0045] In addition, the rack 71 is detachably connected to the frame 1 and the workbench 2 to adapt to different customer needs. Some customers only need to purchase the visual judgment device 7, while others only need to purchase the automatic loading and unloading device 3 and the dyeing treatment device 4. They can be sold separately or in combination according to customer needs, thus adapting to different customer needs.

[0046] The implementation principle of this embodiment is as follows: This fully automated acid-fast Gram slide analysis device achieves full automation from sample loading and staining to analysis through the coordinated work of various mechanisms and components. The automatic loading and unloading device 3 utilizes the feeding component 34, the top component 35, and the discharge component 36 to automatically load the slides and the slide container 31, avoiding the tediousness and inefficiency of manual operation. The first transfer mechanism 5 and the second transfer mechanism 6 accurately transfer the slides between the devices, ensuring a smooth process. The staining treatment device 4's staining agent addition, cleaning, and drying functions can precisely control the staining process and improve the consistency of staining results. The visualization and judgment device 7 observes and analyzes the stained slides through a microscope 73, providing accurate results for bacterial detection. Compared with traditional manual operation and semi-automated equipment, this device improves detection efficiency and accuracy, reduces human error, and meets the ever-increasing detection demands.

[0047] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A fully automated acid-resistant Gram slide reading and analysis device, comprising a frame (1), wherein a horizontal worktable (2) is provided on one side of the frame (1), characterized in that: An automatic loading and unloading device and a dyeing treatment device (4) are sequentially installed on the workbench (2), and a visual judgment device (7) is installed at one end of the frame (1) and the workbench (2); A horizontally arranged rack (11) is provided on the frame (1), and a first transmission mechanism (5) and a second transmission mechanism (6) are provided on the rack (11). The first transmission mechanism (5) is used to transfer the glass slides on the automatic loading and unloading device to the staining treatment device (4), and the second transmission mechanism (6) is used to transfer the glass slides between the staining treatment device (4) and the visualization judgment device (7). The automatic loading and unloading device includes a material box (31) placed on the workbench (2). The material box (31) has multiple slots (311) for inserting glass slides. The bottom of the workbench (2) is provided with a feeding component (34), a top component (35), and a discharge component (36) to push the material box (31) to slide automatically to the bottom of the first transmission mechanism (5). The dyeing treatment device (4) includes a feeding box (41), and a dyeing agent adding mechanism (42), a cleaning mechanism (45) and a drying mechanism (44) are provided on one side of the feeding box (41).

2. The fully automated acid-resistant Gram slide reading and analysis device according to claim 1, characterized in that: The workbench (2) is divided into a feeding area (32) and a discharging area. The feeding area (32) is located on the workbench (2) close to the dyeing treatment device (4), and the discharging area is located on the workbench (2) away from the dyeing treatment device (4). Both the feeding assembly (34) and the discharging assembly (36) include a pusher motor (341) fixedly connected to the bottom of the workbench (2). The output shaft of the pusher motor (341) is fixedly connected to a sliding block (342). The bottom of the workbench (2) is rotatably connected to a feeding screw (343). The sliding block (342) is threadedly connected to the feeding screw (343). The two sides of the sliding block (342) are rotatably connected to a pusher rod (344). The top of the pusher rod (344) is provided with an inclined surface. The inclined surface is inclined upward on the side facing the transmission direction. The workbench (2) is provided with a clearance groove (21) corresponding to the position of the pusher rod (344). The pusher rod (344) extends into the reversing groove. A stop rod (345) is fixedly connected to the sliding block (342). The stop rod (345) is used to limit the pusher rod (344). A connecting channel is provided between the feeding area (32) and the unloading area (33). The top material assembly (35) includes a guide rail (351) fixedly connected to the frame (1). The guide rail (351) is located above the connecting channel. A top material block (354) is slidably connected to the guide rail (351). A top material motor (352) is provided on the frame (1). A pusher screw (353) is fixedly connected to the output shaft of the top material motor (352). The axial direction of the pusher screw (353) is perpendicular to the axial direction of the feeding screw (343). The top material block (354) is threadedly connected to the threaded column (4366).

3. The fully automated acid-resistant Gram slide reading and analysis device according to claim 1, characterized in that: The first feeding mechanism and the second feeding mechanism both include a sliding frame (51) slidably connected to the frame (1), a drive motor (52) is fixedly connected to the sliding frame (51), and a gear (53) is fixedly connected to the output shaft of the drive motor (52), and the gear (53) meshes with the rack (11); A power motor (54) is rotatably connected to the top of the sliding frame (51). A feeding screw (541) is fixedly connected to the output shaft of the power motor (54). The feeding screw (541) is vertically oriented. A picking frame (542) is threadedly connected to the feeding screw (541). A flipping motor (55) is fixedly connected to one side of the picking frame (542). The output shaft of the flipping motor (55) extends into the picking frame (542) and is fixedly connected to a clamping motor (56). An electromagnetic chuck (561) is fixedly connected to the output shaft of the clamping motor (56).

4. The fully automated acid-resistant Gram slide reading and analysis device according to claim 1, characterized in that: The top of the feeding box (41) is provided with a feeding port (411), which is used to facilitate the insertion of glass slides into the feeding box (41); The staining agent adding mechanism (42) includes multiple reagent kits (421) fixedly connected to one side of the workbench (2). Each reagent kit (421) contains various different staining agents. A base plate (422) is fixedly connected to the workbench (2). A stepper motor (423) is fixedly connected to one side of the base plate (422). A first screw (424) is fixedly connected to the output shaft of the stepper motor (423). A guide block (425) is threaded onto the first screw (424). A feed plate (426) is fixedly connected to the guide block (425), and multiple drop pumps (4261) are fixedly connected to the feed plate (426). Each drop pump (4261) is equipped with a dropper (4262), and each drop pump (4261) is fixedly connected with a feed tube (427). The feed tubes (427) are connected to different reagent kits (421). The side wall of the feeding box (41) is provided with a feed port (412) for the feed plate (426) to enter and exit.

5. The fully automated acid-resistant Gram slide reading and analysis device according to claim 1, characterized in that: The feeding box (41) is equipped with a spray head, and a water outlet pipe (451) is provided on one side of the feeding box (41). A pump (452) is connected to the water outlet pipe (451).

6. The fully automated acid-resistant Gram slide reading and analysis device according to claim 1, characterized in that: A support (43) is provided on one side of the base plate (422), a conveyor belt (431) is provided on the support (43), unused droppers (4262) are placed on the conveyor belt (431), and a motor for driving the conveyor belt (431) to slide is provided on the support (43). A second motor (432) is fixedly connected to the side wall of the feeding box (41). A swing arm (433) is fixedly connected to the output shaft of the second motor (432). A rotating shaft (434) is hinged to the end of the swing arm (433). A third motor (435) is fixedly connected to the swing arm (433). The output shaft of the third motor (435) is fixed to the rotating shaft (434). A positioning box (436) is fixedly connected to the rotating shaft (434). The positioning box (436) is slidably connected to a clamping seat (4361), and the end of the clamping seat (4361) is fixedly connected to a plug sleeve (4362), which is used for the insertion of the dropper (4262). Electromagnetic clamping rods (4363) are symmetrically arranged on both sides of the clamping seat (4361). A reset cavity (4364) is opened in the clamping seat (4361). A reset spring (4364) is fixedly connected in the reset cavity (4364). One end of the electromagnetic clamping rod (4363) is fixedly connected to the reset spring (4364), and the other end of the electromagnetic clamping rod (4363) is used to clamp the end of the dropper (4262). A power component for pushing the dropper (4262) to be inserted into the drip pump (4261) is provided in the clamping seat (4361).

7. The fully automated acid-resistant Gram slide reading and analysis device according to claim 6, characterized in that: The power assembly includes a fourth motor (4365) fixed inside the positioning box (436). The fourth motor (4365) is located on the side of the clamping seat (4361) away from the plug-in cylinder. A threaded column (4366) is fixedly connected to the output shaft of the fourth motor (4365), and the clamping seat (4361) is threadedly connected to the threaded column (4366).

8. The fully automated acid-resistant Gram slide reading and analysis device according to claim 7, characterized in that: The power assembly includes a fourth motor (4365) fixed inside the positioning box (436). The fourth motor (4365) is located on the side of the clamping seat (4361) away from the plug-in cylinder. A threaded column (4366) is fixedly connected to the output shaft of the fourth motor (4365), and the clamping seat (4361) is threadedly connected to the threaded column (4366).

9. The fully automated acid-resistant Gram slide reading and analysis device according to claim 1, characterized in that: The workbench (2) is provided with an immersion box (46), and multiple reagent tanks (461) are provided in the immersion box (46). The reagent tanks (461) are used for inserting glass slides. A water pump (462) is provided on one side of the reagent tank (461). A feed pipe (463) is connected to the water pump (462). The feed pipe (463) is connected to the immersion solution. A slide rail (464) is fixedly connected to the workbench (2). A fifth motor (465) is fixedly connected to one side of the slide rail (464). A positioning block (466) is fixedly connected to the output shaft of the fifth motor (465). A feed pipe (463) is fixedly connected to the positioning block (466). The end of the feed pipe (427) extends above the reagent tank (461).

10. The fully automated acid-resistant Gram slide reading and analysis device according to claim 1, characterized in that: The visualization judgment device (7) includes a frame (71), a stage (72) is provided on the frame (71), and a microscope (73) is provided above the stage (72) on the frame (71).

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