Microorganism detection device for food detection

By designing automated clamping, transmission, inoculation, and liquid injection mechanisms, the challenges of automation and aseptic operation in traditional food microbiology testing have been solved, achieving efficient and accurate food microbiology testing.

CN121109105APending Publication Date: 2025-12-12淄博市检验检测计量研究总院
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Patent Information

Application Number
CN202511451759.6
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-10-11
Publication Date
2025-12-12

AI Technical Summary

Technical Problem

Traditional food microbial testing methods cannot achieve automated and aseptic operation, resulting in low testing efficiency, poor accuracy, and susceptibility to secondary contamination.

Method used

A microbial detection device for food testing was designed, which employs a clamping mechanism, a transmission mechanism, a petri dish inoculation mechanism, and a petri dish liquid injection mechanism to achieve automated aseptic dilution of food with physiological saline. Through automated inoculation and liquid injection functions, manual operation steps are reduced and detection accuracy is improved.

Benefits of technology

It enables automated aseptic dilution and inoculation for food microbial testing, reducing the risk of external microbial contamination and improving testing efficiency and accuracy.

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Abstract

The invention discloses a microbiological detection device for food detection, and relates to the field of food microbiological detection.The microbiological detection device comprises a mounting box, a mounting column is slidably connected to the mounting box, and a connecting plate is fixedly connected to the outer surface of the mounting column. Through cooperation of the first rotation driving part, the third telescopic driving part and the like, a volumetric flask plug can be accurately clamped, detection liquid is sucked and quantitatively inoculated to a culture dish, and the culture dish is driven to rotate in a reciprocating manner in the inoculation process, so that the detection liquid is uniformly coated. The culture dish liquid injection mechanism can accurately inject different nutrient solutions and color developing solutions into the culture dish according to a detected flora target, the culture dish is driven to rotate during injection, and uniform mixing is ensured. The jacking mechanism assists in taking, placing and moving of the culture dishes. The whole process is high in automation degree, manual operation steps are reduced, the pollution risk of external strains is reduced, and the accuracy of detection data is further improved.
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Description

TECHNICAL FIELD

[0001] The present application relates to food microbiological detection technology, and in particular to a food detection microbiological detection device. BACKGROUND

[0002] In the food industry, microbiological detection is a key link to ensure food safety and quality. Accurate detection of the content of microorganisms in food can timely find out whether the food is contaminated and the degree of contamination, so as to effectively prevent the occurrence of foodborne diseases and protect the health of consumers.

[0003] The traditional food microbiological detection process has many drawbacks. In the sample dilution link, the food to be detected is usually manually placed in a container containing physiological saline, and then the food and physiological saline are fully mixed by manual shaking or other methods to achieve dilution. However, this manual operation method is not only inefficient, but also difficult to ensure uniformity of mixing, thereby affecting the accuracy of subsequent detection results. More seriously, during the entire operation process, since it cannot be carried out in a completely sterile environment, manual operation is easy to introduce external bacteria, causing the sample to be secondarily contaminated, so that the detection data cannot truly reflect the actual situation of microorganisms in food, and at the same time, in the inoculation and liquid injection link, the traditional method also relies on a large amount of manual operation. For example, using an inoculating loop to pick up samples for inoculation, and using a dropper or other tools for liquid injection. These operations are not only tedious and labor-intensive, but also difficult to accurately control the inoculation amount and liquid injection amount, and are prone to operation errors. At the same time, frequent manual operation also increases the risk of sample contamination, further reducing the reliability of detection data.

[0004] With the rapid development of the food industry and the increasing demand for food safety, the traditional food microbiological detection method has been difficult to meet the actual needs. Therefore, it is of great practical significance to develop a food microbiological detection device that can realize automatic and sterile operation. SUMMARY

[0005] The purpose of the present application is to provide a food detection microbiological detection device to solve the problem that the prior art cannot realize automatic and sterile operation, resulting in poor food microbiological detection effect.

[0006] In order to achieve the above object, the present application provides the following technical scheme: a kind of microbial detection device for food detection, including installation box, the installation box is slidably connected with installation column, the outer surface of the installation column is fixedly connected with connecting plate, the side of the connecting plate is fixedly connected with the first telescopic drive element fixedly connected with installation box, the installation column is rotatably connected with transmission column, the top of the transmission column is connected with the fixed frame rotatably connected with installation box by transmission mechanism, the transmission mechanism is used for the connection between fixed frame and transmission column, clamping mechanism is provided in the fixed frame; Limiting column is fixedly connected in the installation box, a plurality of culture dishes are slidably connected on the limiting column, iron ring is provided on the culture dish, the outer surface of the transmission column is fixedly connected with electromagnet ring by connecting rod, the bottom of the installation box is provided with jacking mechanism, the jacking mechanism is used to drive the culture dish on the limiting column to move up and down, culture dish inoculation mechanism is provided in the installation box, the culture dish inoculation mechanism is used to quantitatively inoculate the material in sample bottle in fixed frame into each culture dish, the bottom of the culture dish is fixedly connected with cover plate; The top of the installation box is threadedly connected with a plurality of culture dish liquid injection mechanisms, the culture dish liquid injection mechanism is used to inject liquid to the inoculated culture dish, the outer surface of the transmission column is drivingly connected with the drive mechanism connected with the connecting plate, and the drive mechanism is used to drive the transmission column to rotate.

[0007] Further, the transmission mechanism includes a connecting shaft fixedly connected with the fixed frame, the bottom end of the connecting shaft is fixedly connected with a connecting box, a plurality of connecting columns are slidably connected in the connecting box, springs fixedly connected with the connecting box are fixedly sleeved on the outer surface of the connecting column, the top end of the transmission column is fixedly connected with a connecting disc, and a transmission groove slidably connected with the connecting column is formed in the top of the connecting disc.

[0008] Further, the clamping mechanism includes a fixed gas bag fixedly connected in the fixed frame, the outer surface of the fixed gas bag is fixedly connected with the air supply box fixedly connected with the installation box through pipeline, the second telescopic drive element is fixedly connected in the air supply box, and the output end of the second telescopic drive element is fixedly connected with the extrusion plate slidably connected with the air supply box.

[0009] Further, the culture dish inoculation mechanism comprises a first rotating drive member fixedly connected with the mounting box, an output end of the first rotating drive member is fixedly connected with a first threaded rod in rotating connection with the mounting box, an outer surface of the first threaded rod is threadedly matched with a transmission plate, the transmission plate is slidably connected with a guide rod fixedly connected with the mounting box, a bottom of the transmission plate is fixedly connected with a third telescopic drive member, an output end of the third telescopic drive member is fixedly connected with a moving plate, the moving plate is provided with a first threaded groove, the first threaded groove is threadedly connected with a first threaded fixing ring, the first threaded fixing ring is fixedly connected with an inoculation syringe, a top of the inoculation syringe is fixedly connected with an iron plate, a top of the moving plate is fixedly connected with a fourth telescopic drive member, an output end of the fourth telescopic drive member is fixedly connected with an electromagnet block, a bottom of the moving plate is fixedly connected with a clamping telescopic drive member, an output end of the clamping telescopic drive member is fixedly connected with a clamping block.

[0010] Further, the culture dish liquid injection mechanism comprises a plurality of second threaded grooves formed in the top of the mounting box, the second threaded grooves are threadedly connected with second threaded fixing rings, inner surfaces of the second threaded fixing rings are fixedly connected with liquid supply syringes, a top of the mounting box is fixedly connected with a plurality of second rotating drive members, output ends of the second rotating drive members are fixedly connected with second threaded rods, outer surfaces of the second threaded rods are threadedly matched with pressing plates, the pressing plates are slidably connected with guide rods fixedly connected with the mounting box.

[0011] Further, the jacking mechanism comprises a transmission box fixedly connected with the mounting box, the transmission box is fixedly connected with a third rotating drive member, an output end of the third rotating drive member is fixedly connected with a transmission shaft, an outer surface of the transmission shaft is fixedly sleeved with a transmission gear, the transmission gear is meshingly connected with two transmission racks, one side of the transmission racks is fixedly connected with lifting columns in sliding connection with the mounting box, a top end of the lifting column is fixedly connected with a top plate.

[0012] Further, the driving mechanism comprises a fourth rotating drive member fixedly connected with the connecting plate, an output end of the fourth rotating drive member is fixedly connected with a driving shaft, an outer surface of the driving shaft is fixedly sleeved with a first driving gear, the first driving gear is meshingly connected with a second driving gear fixedly sleeved with the transmission column.

[0013] Further, the mounting box is rotatably connected with an opening and closing door.

[0014] Compared with the prior art, the microbial detection device for food detection provided by the application has the following beneficial effects: The device realizes automatic and sterile dilution of food detection through ingenious design. First, the culture dish is placed in the installation box for sterilization and disinfection, the food to be detected is placed in the volumetric flask and placed in the fixed frame, and the volumetric flask is fixed flexibly by using the clamping mechanism. The connecting plate, mounting column and transmission column are moved by the first telescopic driving part, and the transmission column is rotated by cooperating with the driving mechanism, and the connecting disc cooperates with the connecting column and spring to realize the rotation of the fixed frame driving the volumetric flask, so that the physiological saline and the food are fully mixed, and the automatic and sterile dilution is completed. The whole process is carried out in a closed and sterile environment, which avoids the pollution caused by manual operation, has high automation degree, reduces manual intervention, provides good conditions for subsequent inspection operation, and effectively improves the detection efficiency and accuracy.

[0015] The device has perfect automatic inoculation and liquid injection functions. The culture dish inoculation mechanism can accurately clamp the volumetric flask stopper, suck the detection liquid and inoculate the culture dish quantitatively by cooperating with the first rotary driving part and the third telescopic driving part, and rotate the culture dish reciprocally during the inoculation process to evenly coat the detection liquid. The culture dish liquid injection mechanism can accurately inject different nutrient solutions and color developing solutions into the culture dish according to the target of detection bacteria group, and also rotates the culture dish during injection to ensure uniform mixing. The jacking mechanism assists the taking and moving of the culture dish. The whole process has high automation degree, reduces manual operation steps, reduces the risk of external bacterial contamination, further improves the accuracy of detection data, and provides strong support for food microbiological detection. BRIEF DESCRIPTION OF DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed in the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments described in the present application, and other drawings can also be obtained by those skilled in the art according to these drawings.

[0017] Figure 1 It is the first perspective view of the external structure of the present application. Figure 2 It is the second perspective view of the external structure of the present application. Figure 3 It is the first perspective view of the internal structure of the present application. Figure 4 It is the second perspective view of the internal structure of the present application. Figure 5 It is the first front view of the internal structure of the present application. Figure 6 It is the second front view of the internal structure of the present application. Figure 7 It is the first perspective view of the internal structure of the present application. Figure 3 It is the second perspective view of the internal structure of the present application. Figure 8 Figure 3 It is the second perspective view of the internal structure of the present application.​ Figure 9 For the present invention Figure 5 A magnified view of C.

[0018] Explanation of reference numerals in the attached figures: 1. Mounting box; 2. Mounting column; 3. Connecting plate; 4. First telescopic drive component; 5. Transmission column; 6. Fixing frame; 7. Limiting column; 8. Petri dish; 9. Iron ring; 10. Electromagnetic ring; 11. Cover plate; 21. Connecting shaft; 22. Connecting box; 23. Connecting column; 24. Spring; 25. Connecting plate; 26. Transmission groove; 31. Fixing airbag; 32. Air supply box; 33. Second telescopic drive component; 34. Extrusion plate; 41. First rotation drive component; 42. First threaded rod; 43. Transmission plate; 44. Third telescopic drive component; 45. Moving plate; 46. First threaded groove; 47. First threaded fixing rod. 48. Fixed ring; 49. Inoculation syringe; 40. Iron plate; 41. Third telescopic drive component; 42. Electromagnet block; 43. Clamping telescopic drive component; 54. Clamping block; 55. Second threaded groove; 56. Second threaded fixing ring; 57. Liquid supply syringe; 68. Second rotation drive component; 59. Second threaded rod; 60. Pressing plate; 61. Transmission box; 62. Third rotation drive component; 63. Transmission shaft; 64. Transmission gear; 65. Transmission rack; 66. Lifting column; 67. Top plate; 78. Fourth rotation drive component; 79. Drive shaft; 70. First drive gear; 71. Second drive gear. Detailed Implementation

[0019] To enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings. Example

[0020] This invention provides a microbial detection device for food testing, including a mounting box 1, a mounting column 2 slidably connected to the mounting box 1, a connecting plate 3 fixedly connected to the outer surface of the mounting column 2, a first telescopic drive member 4 fixedly connected to one side of the connecting plate 3 and fixedly connected to the mounting box 1, the first telescopic drive member 4 being an electric telescopic rod, a transmission column 5 rotatably connected to the mounting column 2, and a fixed frame 6 rotatably connected to the mounting box 1 via a transmission mechanism at the top end of the transmission column 5, the transmission mechanism being used for the connection between the fixed frame 6 and the transmission column 5, and a clamping mechanism being provided inside the fixed frame 6; The mounting box 1 is fixedly connected to a limiting column 7, and multiple petri dishes 8 are slidably connected to the limiting column 7. Iron rings 9 are set on the petri dishes 8. An electromagnet ring 10 is fixedly connected to the outer surface of the transmission column 5 through a connecting rod. A lifting mechanism is set at the bottom of the mounting box 1. The lifting mechanism is used to drive the petri dishes 8 on the limiting column 7 to move up and down. A petri dish inoculation mechanism is set inside the mounting box 1. The petri dish inoculation mechanism is used to quantitatively inoculate the material in the sample bottle in the fixed frame 6 into each petri dish 8. A cover plate 11 is fixedly connected to the bottom of the petri dish 8. The top of the mounting box 1 is threaded with multiple culture dish injection mechanisms, which are used to inject liquid into the culture dishes after inoculation. The outer surface of the transmission column 5 is connected to a drive mechanism that is connected to the connecting plate 3. The drive mechanism is used to drive the transmission column 5 to rotate. The mounting box 1 is rotatably connected with an opening and closing door.

[0021] The transmission mechanism includes a connecting shaft 21 fixedly connected to the fixed frame 6. A connecting box 22 is fixedly connected to the bottom end of the connecting shaft 21. Multiple connecting columns 23 are slidably connected inside the connecting box 22. A spring 24 fixedly sleeved on the outer surface of the connecting column 23 and fixedly connected to the connecting box 22 is fixedly fitted. A connecting plate 25 is fixedly connected to the top end of the transmission column 5. A transmission groove 26 is opened on the top of the connecting plate 25 and slidably connected to the connecting column 23.

[0022] The clamping mechanism includes a fixed airbag 31 fixedly connected inside the fixed frame 6. The outer surface of the fixed airbag 31 is fixedly connected to an air supply box 32 fixedly connected to the mounting box 1 via a pipe. A second telescopic drive member 33 is fixedly connected inside the air supply box 32. The second telescopic drive member 33 is an electric telescopic rod. The output end of the second telescopic drive member 33 is fixedly connected to a compression plate 34 slidably connected to the air supply box 32. The compression plate 34 is moved by the second telescopic drive member 33 inside the air supply box 32, and the compression plate 34 compresses the gas in the air supply box 32. At this time, the gas in the air supply box 32 enters the fixed airbag 31 through the pipe, causing the fixed airbag 31 to inflate.

[0023] The drive mechanism includes a fourth rotation drive component 71 fixedly connected to the connecting plate 3. The fourth rotation drive component 71 is a servo motor, which is controlled by a PLC programming program. The servo motor can be controlled to rotate forward and backward and rotate at different angles. The output end of the fourth rotation drive component 71 is fixedly connected to a drive shaft 72. A first drive gear 73 is fixedly sleeved on the outer surface of the drive shaft 72. A second drive gear 74, which is fixedly sleeved on the outer surface of the first drive gear 73, is meshed with the outer surface of the first drive gear 73. The fourth rotation drive component 71 drives the drive shaft 72 to rotate, and the drive shaft 72 drives the transmission column 5 to rotate through the first drive gear 73 and the second drive gear 74.

[0024] First, the interior of the installation box 1 is thoroughly sterilized. Then, the petri dish inoculation mechanism and petri dish dispensing mechanism are installed. Next, multiple sterilized petri dishes 8 are stacked on the limiting column 7. The installation box 1 is then sterilized again. The food to be tested is then placed into a volumetric flask containing physiological saline, and the flask opening is sealed with a stopper. The volumetric flask is then placed into the fixing frame 6. The clamping mechanism inflates the fixing airbag 31, which flexibly compresses and fixes the volumetric flask. Then, the first telescopic drive 4 moves the connecting plate 3 and the mounting column 2 upwards. The mounting column 2 moves the transmission column 5 upwards, which in turn moves the connecting plate 25 upwards. The disk 25 pushes the connecting column 23 at the bottom of the connecting box 22, causing the connecting column 23 to move upward. At the same time, the spring 24 on the connecting column 23 is compressed, which then drives the transmission column 5 to rotate through the drive mechanism. The transmission column 5 drives the connecting disk 25 to rotate. When the transmission groove 26 on the connecting disk 25 coincides with the connecting column 23, the connecting column 23 enters the transmission groove 26 under the force of the spring 24. At this time, the connecting disk 25 drives the connecting column 23, the connecting box 22, the connecting shaft 21, and the fixing frame 6 to rotate. The fixing frame 6 drives the volumetric flask held in it to rotate, so that the physiological saline in the volumetric flask is fully mixed with the food substances, and the food to be tested is diluted. This realizes the automated aseptic dilution of the food to be tested, which facilitates subsequent inspection operations. Example

[0025] Based on Example 1, please refer to Figures 2 to 9As shown, the petri dish inoculation mechanism includes a first rotation drive 41 fixedly connected to the mounting box 1. The first rotation drive 41 is a servo motor, which is controlled by a PLC programming program to control forward and reverse rotation and rotation angle. The output end of the first rotation drive 41 is fixedly connected to a first threaded rod 42 rotatably connected to the mounting box 1. A transmission plate 43 is threadedly fitted onto the outer surface of the first threaded rod 42. A guide rod fixedly connected to the mounting box 1 is slidably connected to the transmission plate 43. A third telescopic drive 44 is fixedly connected to the bottom of the transmission plate 43. The third telescopic drive 44 is an electric telescopic rod. A moving plate 45 is fixedly connected to the output end of the third telescopic drive 44. A first threaded groove 46 is opened on the moving plate 45, and a first threaded fixing rod is threadedly connected to the first threaded groove 46. A first threaded fixing ring 47 is fixedly connected to an inoculation syringe 48. An iron plate 49 is fixedly connected to the top of the inoculation syringe 48. A fourth telescopic drive 490 is fixedly connected to the top of a movable plate 45. The fourth telescopic drive 490 is an electric telescopic rod. An electromagnet block 491 is fixedly connected to the output end of the fourth telescopic drive 490. A clamping telescopic drive 492 is fixedly connected to the bottom of the movable plate 45. The clamping telescopic drive 492 is an electric telescopic rod. A clamping block 493 is fixedly connected to the output end of the clamping telescopic drive 492. By threading the first threaded fixing ring 47 on the surface of the inoculation syringe 48 into the first threaded groove 46 on the movable plate 45, a threaded connection is formed, which facilitates the subsequent disassembly and assembly of the inoculation syringe 48, thereby facilitating the cleaning and sterilization of the inoculation syringe 48.

[0026] The culture dish injection mechanism includes multiple second threaded grooves 51 on the top of the mounting box 1. A second threaded retaining ring 52 is threadedly connected to the inner thread of the second threaded groove 51. A liquid injection syringe 53 is fixedly connected to the inner surface of the second threaded retaining ring 52. Multiple second rotation drive components 54 are fixedly connected to the top of the mounting box 1. The second rotation drive component 54 is a servo motor. The servo motor is controlled by a PLC programming program, which can control the servo motor to rotate forward and backward and the rotation angle. A second threaded rod 55 is fixedly connected to the output end of the second rotation drive component 54. A pressing plate 56 is threadedly fitted to the outer surface of the second threaded rod 55. A guide rod fixedly connected to the mounting box 1 is slidably connected to the pressing plate 56.

[0027] According to the target bacterial species to be detected, the nutrient solution of different target bacterial species is put into the liquid supply syringe 53 respectively. Then, the liquid supply syringe 53 is threaded into the corresponding second thread groove 51. At the same time, the color development solution is put into the liquid supply syringe 53 and installed in the second thread groove 51. Meanwhile, the second thread groove 51 that does not need to be installed is sealed by the installation block.

[0028] The lifting mechanism includes a transmission box 61 fixedly connected to the mounting box 1. A third rotation drive component 62 is fixedly connected inside the transmission box 61. The third rotation drive component 62 is a servo motor, which is controlled by a PLC programming program. The servo motor can be controlled to rotate forward and backward and rotate at different angles. A transmission shaft 63 is fixedly connected to the output end of the third rotation drive component 62. A transmission gear 64 is fixedly sleeved on the outer surface of the transmission shaft 63. Two transmission racks 65 are meshed on the outer surface of the transmission gear 64. A lifting column 66, which is slidably connected to the mounting box 1, is fixedly connected to one side of the transmission rack 65 through a connecting block. A top plate 67 is fixedly connected to the top of the lifting column 66. The third rotation drive component 62 drives the transmission shaft 63 to rotate, which in turn drives the transmission gear 64 to rotate. The transmission gear 64 drives the two transmission racks 65 to move, and the two transmission racks 65 drive the lifting columns 66 on both sides to rise and fall simultaneously.

[0029] After the food testing solution in the volumetric flask is thoroughly mixed, the first telescopic drive 4 moves the connecting plate 3 downwards, thereby moving the mounting column 2 and the transmission column 5 downwards. This causes the connecting plate 25 to disengage from the transmission column 5. Subsequently, the drive mechanism rotates the mounting column 2 and the transmission column 5, causing the transmission column 5 to rotate and position the electromagnet ring 10 above multiple culture dishes 8. Then, the lifting mechanism lifts one of the lifting columns 66 and the top plate 67 upwards by the distance of one culture dish 8. Simultaneously, the other lifting column 66 moves the top plate 67 downwards by the position of one culture dish 8. Then, the first telescopic drive 4 moves the mounting column 2 and the transmission column 5 downwards, and the electromagnet ring 10 is energized to attract and fix the culture dishes 8 at the top. Then, the culture dish 8 moves upward, and the drive mechanism drives the transmission column 5 to rotate, causing the culture dish 8, which is fixed in place, to rotate below the culture dish inoculation mechanism. Then, the first rotating drive member 41 drives the first threaded rod 42 to rotate, and the first threaded rod 42 drives the transmission plate 43 to move, causing the clamping block 493 on the moving plate 45 to move above the volumetric flask. Then, the third telescopic drive member 44 drives the moving plate 45 to move downward, and the clamping telescopic drive member 492 drives the clamping block 493 to clamp the stopper of the volumetric flask. Then, the moving plate 45 moves downward to remove the stopper. Then, the third telescopic drive member 44 drives the moving plate 45 to move upward, simultaneously moving the transmission plate 43, causing the inoculation syringe 4 to move upward. The syringe 48 is moved above the volumetric flask, and then the needle of the inoculation syringe 48 is inserted into the volumetric flask. The electromagnet block 491 then attracts the iron plate 49 on top of the inoculation syringe 48. The fourth telescopic drive 490 moves the inoculation syringe 48 to draw in the test solution from the volumetric flask. The inoculation syringe 48 is then moved above the culture dish 8 attached to the electromagnet ring 10. The fourth telescopic drive 490 moves the inoculation syringe 48 to quantitatively inoculate the test solution into the culture dish 8. Simultaneously, during inoculation, the reciprocating movement of the transmission plate 43 and the reciprocating rotation of the transmission column 5 cause the culture dish 8 to rotate, ensuring the inoculated test solution is evenly distributed on the culture dish. Artificial inoculation was simulated on the culture dish 8, ensuring even inoculation within the dish. After inoculation, the culture dish 8 was rotated according to the target bacterial population, causing the injection syringe 53 containing the culture medium to be injected. Subsequently, the second rotating drive 54 rotated the second threaded rod 55, which in turn moved the pressing plate 56 downwards, injecting the nutrient solution from the injection syringe 53 into the culture dish 8. During injection, the culture dish 8 was simultaneously rotated back and forth, ensuring even mixing of the culture medium with the inoculated detection solution. The dish was then rotated to the injection syringe 53 containing the colorimetric solution, injecting the solution into the culture dish 8 to develop the color of the bacteria for subsequent detection. Finally, the top of the other pair of limiting columns 7 was rotated.The petri dish 8 is then inserted into the limiting column 7 and placed onto the top plate 67. This process is repeated sequentially to automate the inoculation and liquid injection for the detection of different microbial strains in food. After all inoculation and liquid injection are completed, the inoculated and injected petri dish 8 is removed and placed in an incubator for further microbial testing. This achieves automated inoculation and liquid injection for food microbial testing, further improving the automation level of food microbial testing, reducing manual operation steps, minimizing external microbial contamination, and further improving the accuracy of test data.

[0030] Working principle: First, the interior of the installation box 1 is thoroughly sterilized. Then, the petri dish inoculation mechanism and petri dish liquid injection mechanism are installed. Next, multiple sterilized petri dishes 8 are stacked on the limiting column 7. The installation box 1 is then sterilized again. The food to be tested is then placed into a volumetric flask containing physiological saline, and the flask opening is plugged. The volumetric flask is then placed into the fixing frame 6. The clamping mechanism inflates the fixing airbag 31, which flexibly compresses and fixes the volumetric flask. Then, the first telescopic drive component 4 moves the connecting plate 3 and the mounting column 2 upwards. The mounting column 2 moves the transmission column 5 upwards, which in turn moves the connecting plate 25 upwards. The connecting plate 25 pushes the connecting column 23 at the bottom of the connecting box 22, causing the connecting column 23 to move upward. At the same time, the spring 24 on the connecting column 23 is compressed, which then drives the transmission column 5 to rotate through the drive mechanism. The transmission column 5 drives the connecting plate 25 to rotate. When the transmission groove 26 on the connecting plate 25 coincides with the connecting column 23, the connecting column 23 enters the transmission groove 26 under the force of the spring 24. At this time, the connecting plate 25 drives the connecting column 23, the connecting box 22, the connecting shaft 21, and the fixing frame 6 to rotate. The fixing frame 6 drives the clamped volumetric flask to rotate, so that the physiological saline in the volumetric flask is fully mixed with the food substances, and the food to be tested is diluted. This realizes the automated aseptic dilution of the food to be tested, which facilitates subsequent inspection operations.

[0031] The foregoing has only described certain exemplary embodiments of the present invention by way of illustration. Undoubtedly, those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the foregoing drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A microbial detection device for food testing, characterized in that, The system includes a mounting box (1), on which a mounting column (2) is slidably connected. A connecting plate (3) is fixedly connected to the outer surface of the mounting column (2). A first telescopic drive member (4) fixedly connected to the mounting box (1) is fixedly connected to one side of the connecting plate (3). A transmission column (5) is rotatably connected to the mounting column (2). A fixed frame (6) rotatably connected to the mounting box (1) is driven to the top of the transmission column (5) through a transmission mechanism. The transmission mechanism is used for the connection between the fixed frame (6) and the transmission column (5). A clamping mechanism is provided inside the fixed frame (6). The mounting box (1) is fixedly connected to a limiting column (7), and multiple petri dishes (8) are slidably connected to the limiting column (7). An iron ring (9) is provided on the petri dish (8). An electromagnet ring (10) is fixedly connected to the outer surface of the transmission column (5) through a connecting rod. A lifting mechanism is provided at the bottom of the mounting box (1). The lifting mechanism is used to drive the petri dishes (8) on the limiting column (7) to move up and down. A petri dish inoculation mechanism is provided in the mounting box (1). The petri dish inoculation mechanism is used to quantitatively inoculate the material in the sample bottle in the fixed frame (6) into each petri dish (8). A cover plate (11) is fixedly connected to the bottom of the petri dish (8). The top of the mounting box (1) is threaded with multiple culture dish injection mechanisms, which are used to inject liquid into the culture dish after inoculation. The outer surface of the transmission column (5) is connected to a drive mechanism connected to the connecting plate (3), which is used to drive the transmission column (5) to rotate.

2. The microbial detection device for food testing according to claim 1, characterized in that, The transmission mechanism includes a connecting shaft (21) fixedly connected to the fixed frame (6), a connecting box (22) fixedly connected to the bottom end of the connecting shaft (21), a plurality of connecting columns (23) slidably connected inside the connecting box (22), a spring (24) fixedly sleeved on the outer surface of the connecting column (23) and fixedly connected to the connecting box (22), a connecting plate (25) fixedly connected to the top end of the transmission column (5), and a transmission groove (26) slidably connected to the connecting column (23) is opened on the top of the connecting plate (25).

3. The microbial detection device for food testing according to claim 1, characterized in that, The clamping mechanism includes a fixed airbag (31) fixedly connected inside a fixed frame (6). The outer surface of the fixed airbag (31) is fixedly connected to an air supply box (32) fixedly connected to the mounting box (1) via a pipe. A second telescopic drive member (33) is fixedly connected inside the air supply box (32). The output end of the second telescopic drive member (33) is fixedly connected to a compression plate (34) that is slidably connected to the air supply box (32).

4. The microbial detection device for food testing according to claim 1, characterized in that, The petri dish inoculation mechanism includes a first rotating drive (41) fixedly connected to the mounting box (1). The output end of the first rotating drive (41) is fixedly connected to a first threaded rod (42) rotatably connected to the mounting box (1). The outer surface of the first threaded rod (42) is threadedly fitted with a transmission plate (43). A guide rod fixedly connected to the mounting box (1) is slidably connected to the transmission plate (43). A third telescopic drive (44) is fixedly connected to the bottom of the transmission plate (43). A movable plate (45) is fixedly connected to the output end of the third telescopic drive (44). A first thread is provided on the movable plate (45). The groove (46) is threaded with a first threaded retaining ring (47), and an inoculation syringe (48) is fixedly connected inside the first threaded retaining ring (47). An iron plate (49) is fixedly connected to the top of the inoculation syringe (48). A fourth telescopic drive (490) is fixedly connected to the top of the moving plate (45). An electromagnet block (491) is fixedly connected to the output end of the fourth telescopic drive (490). A clamping telescopic drive (492) is fixedly connected to the bottom of the moving plate (45). A clamping block (493) is fixedly connected to the output end of the clamping telescopic drive (492).

5. A microbial detection device for food testing according to claim 1, characterized in that, The culture dish injection mechanism includes multiple second threaded grooves (51) opened on the top of the mounting box (1). A second threaded retaining ring (52) is threadedly connected to the second threaded groove (51). A liquid injection syringe (53) is fixedly connected to the inner surface of the second threaded retaining ring (52). Multiple second rotating drive components (54) are fixedly connected to the top of the mounting box (1). A second threaded rod (55) is fixedly connected to the output end of the second rotating drive component (54). A pressing plate (56) is threadedly fitted to the outer surface of the second threaded rod (55). A guide rod fixedly connected to the mounting box (1) is slidably connected to the pressing plate (56).

6. The microbial detection device for food testing according to claim 1, characterized in that, The lifting mechanism includes a transmission box (61) fixedly connected to the mounting box (1). A third rotation drive (62) is fixedly connected inside the transmission box (61). A transmission shaft (63) is fixedly connected to the output end of the third rotation drive (62). A transmission gear (64) is fixedly sleeved on the outer surface of the transmission shaft (63). Two transmission racks (65) are meshed on the outer surface of the transmission gear (64). A lifting column (66) that is slidably connected to the mounting box (1) is fixedly connected to one side of the transmission rack (65) through a connecting block. A top plate (67) is fixedly connected to the top of the lifting column (66).

7. A microbial detection device for food testing according to claim 1, characterized in that, The drive mechanism includes a fourth rotation drive member (71) fixedly connected to the connecting plate (3). The output end of the fourth rotation drive member (71) is fixedly connected to a drive shaft (72). A first drive gear (73) is fixedly sleeved on the outer surface of the drive shaft (72). A second drive gear (74) is fixedly sleeved on the outer surface of the first drive gear (73) and meshed with the transmission column (5).

8. A microbial detection device for food testing according to claim 1, characterized in that, The mounting box (1) is rotatably connected to an opening and closing door.