A method and system for blind unmixing of spatially co-localized fluorescent multispectral images

Abstract

The present application relates to a kind of space co-localization's fluorescence multispectral image blind unmixing method and system.The spectral asymmetry of fluorescent protein, the spectral sparsity of fluorescent protein, the characteristics of protein kurtosis continuity are used to estimate the spectral curve of fluorescent protein, combined with the unmixing principle of minimum mutual information, fluorescence multispectral image blind unmixing is carried out, without reference prior fluorescent protein spectral curve, thereby solve the technical problem that existing unmixing method cannot carry out space co-localization's fluorescence multispectral image unmixing without fluorescent protein spectral curve.The problem to be solved by the present application is to overcome the problem that existing spectral overlapping fluorescent dye cannot be blindly unmixing when co-expressed.The spectral curve of no-reference, blind estimation is used to de-constrain existing mutual information minimization unmixing algorithm, to realize the unmixing of co-localization fluorescent protein.The present application has complete blind source separation unmixing capability, and can complete unmixing without using reference spectrum curve.

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