Tissue culture seedling self-adaptive seedling hardening and domestication cabin based on light quality regulation and control and CO2 gradient induction

Abstract

The invention discloses a tissue culture seedling self-adaptive seedling hardening and domestication cabin based on light quality regulation and control and CO2 gradient induction. The domestication cabin comprises a closed cabin body and an environment regulation and control system arranged in the closed cabin body, the environment regulation and control system comprises a control module, a monitoring module and an adjusting module. The control module is used for storing seedling hardening programs corresponding to different crops, controlling the adjusting module to dynamically adjust the domestication environment in the closed cabin according to the environment monitoring parameters provided by the monitoring module and parameter regulation and control curves recorded in the seedling hardening programs, and correcting the change gradient of the parameter regulation and control curves according to the transpiration rate provided by the monitoring module. According to the invention, the environment regulation and control system is integrated in the closed cabin body, and the parameters such as illumination, CO2 concentration, temperature and humidity in the cabin body are adaptively adjusted through the parameter regulation and control curve with gradient change, so that the dynamic induction process of tissue culture seedlings from a laboratory environment to a natural environment is optimized, and the seedling hardening survival rate and the seedling body quality are improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of intelligent seedling cultivation, and in particular to a tissue culture seedling self-adaptive hardening cabin based on light quality regulation and CO2 gradient induction. BACKGROUND

[0002] Hardening refers to a transitional exercise process in which the conditions of the seedling environment are artificially changed before the plant seedlings are transplanted to the final growth environment (for example, a field), such as through sudden humidity reduction, light intensity mutation, carbon dioxide deficiency, and other factors to jointly trigger strong physiological stress on the plants, so that the plants are close to the real growth environment, thereby improving the seedling stress resistance and ensuring the survival rate of transplantation.

[0003] In the prior art, the hardening methods usually include greenhouse hardening and seedling box hardening. The greenhouse hardening is usually directly performed in a greenhouse, and the implementation process of the greenhouse hardening is as follows: the tissue culture bottle is moved to the greenhouse, is exposed to the air for several days to adapt to the humidity change, then the root culture medium is washed away, and is transplanted to a seedling plug, and the environment is regulated by relying on the original shading net, spraying system and natural ventilation of the greenhouse. The greenhouse hardening has the following problems: it relies on the original facilities, natural environment and manual intervention of the operator to regulate the environment, and can only achieve rough humidity and shading, and cannot accurately control the key parameters such as light, gas concentration, temperature and humidity, resulting in high mortality of the tissue culture seedlings due to environmental mutation stress, uneven seedling quality, and inability to directionally improve the effective components in the plants (for example, medicinal components in plants with medicinal and edible properties).

[0004] The seedling box hardening usually sets a closed box body integrated with a heater, a humidifier, a fluorescent lamp and a fan, and sets fixed temperature, humidity and light time parameters by a single-chip microcomputer and keeps them constant. The implementation process is to provide a stable and protected transitional environment for the tissue culture seedlings. The existing seedling box hardening has the following problems: the existing seedling box hardening has limited functions and can only provide static environmental settings, and cannot simulate the dynamic gradient change process required for hardening; the light source in the box usually adopts full-spectrum white light, cannot regulate and control the ratio of specific light qualities (such as red and blue light or UV-A), and generally lacks CO2 supplement function, and cannot actively induce seedlings to exercise stress resistance and effectively regulate the synthesis of secondary metabolites, so the effect is limited in terms of stress resistance exercise and active improvement of seedling quality. SUMMARY

[0005] In view of the above problems, the present application provides a tissue culture seedling self-adaptive hardening cabin based on light quality regulation and CO2 gradient induction, to solve the problems that the existing hardening methods cannot dynamically and comprehensively simulate the real seedling growth environment, affect the effect of stress resistance exercise and quality improvement of the tissue culture seedlings, and result in low survival rate of the transplanted tissue culture seedlings and uneven seedling quality.

[0006] The application provides a tissue culture seedling self-adaptive hardening cabin based on light quality regulation and CO2 gradient induction, which comprises a closed cabin body and an environment regulation system arranged in the closed cabin body; the environment regulation system comprises a control module, a monitoring module and an adjusting module connected with the control module respectively; the control module is used for storing hardening programs corresponding to different crops, controlling the adjusting module to dynamically adjust the hardening environment in the closed cabin body according to an environment monitoring parameter provided by the monitoring module and a parameter regulation curve recorded in the hardening program, and correcting the change gradient of the parameter regulation curve according to a transpiration rate provided by the monitoring module; wherein the parameter regulation curve comprises a light regulation curve used for at least recording dynamic changes of light proportion, light intensity and light cycle required by the crops in different hardening stages, a CO2 regulation curve used for at least recording dynamic changes of a CO2 target concentration required by the crops in different hardening stages, a humidity regulation curve used for at least recording dynamic changes of a humidity target value required by the crops in different hardening stages and a temperature regulation curve used for at least recording dynamic changes of a temperature target value required by the crops in different hardening stages.

[0007] Optionally, the adjusting module comprises a multi-waveband light source, the multi-waveband light source is provided with a plurality of light emitting units of different wavebands, and the light emitting units are one-to-one corresponding to drivers; the control module is configured to determine a driving control signal of any driver according to the light quality proportion, the light intensity and the light cycle, and to close-loop adjust the driving control signal according to a light measured value in the environment monitoring parameter.

[0008] Optionally, the multi-waveband light source is formed by combination of ultraviolet light waveband light emitting units, blue light waveband light emitting units, red light waveband light emitting units, far red light waveband light emitting units and full-spectrum light emitting units; and the light source type of the multi-waveband light source adopts any one or a plurality of combinations of LED light sources, OLED light sources and laser diodes.

[0009] Optionally, the adjusting module comprises a CO2 storage tank and a CO2 injection pipeline, the CO2 injection pipeline is connected with the CO2 storage tank through a flow controller; the control module is configured to compare the CO2 target concentration with a CO2 concentration measured value in the environment monitoring parameter in a light stage, and to determine a flow preset value of the flow controller according to a comparison result; and the flow controller is configured to adjust the CO2 gas flow through the CO2 injection pipeline according to the flow preset value.

[0010] Optionally, the CO2 injection pipeline adopts a ring-shaped diffusion pipe structure; the diffusion pipe is provided with micropores, and the micropores are uniformly distributed in a bottom space of the closed cabin body.

[0011] Optionally, the adjusting module comprises: a humidifying unit and a refrigeration dehumidifying unit arranged in parallel; the humidifying unit adopts at least one of ultrasonic humidification, high-pressure micro-fog or wet membrane evaporation to form atomized particles with a diameter less than 5 microns; the refrigeration dehumidifying unit is arranged on the cabin side wall of the closed cabin, the cold end of the refrigeration dehumidifying unit faces the internal space of the closed cabin, the hot end of the refrigeration dehumidifying unit is arranged in the external space of the closed cabin, and the hot end of the refrigeration dehumidifying unit is provided with a heat dissipation fin and a fan; the control module is configured to: compare the humidity target value with the humidity measured value in the environment monitoring parameter, and control the humidifying unit to perform humidification or control the refrigeration dehumidifying unit to perform dehumidification according to the comparison result.

[0012] Optionally, the adjusting module comprises: a temperature control unit; the temperature control unit is arranged on the cabin side wall of the closed cabin, and both ends of the temperature control unit are connected with heat sinks; the control module is configured to: compare the temperature target value with the temperature measured value in the environment monitoring parameter, and determine the current direction and current size of the driving current of the temperature control unit according to the comparison result.

[0013] Optionally, the monitoring module comprises a transpiration rate monitoring unit; the control module is configured to: determine the real-time transpiration rate of the crop according to the measured value provided by the transpiration rate monitoring unit, and correct the change gradient of the parameter control curve when the real-time transpiration rate exceeds a preset rate threshold.

[0014] Optionally, the closed cabin adopts a double-layer heat insulation structure: the double-layer heat insulation structure comprises: an inner wall plate arranged on the side facing the internal space of the closed cabin and made of a reflective material; an outer wall plate arranged on the side facing the external space of the closed cabin and made of an engineering plastic; and a heat insulation layer arranged between the inner wall plate and the outer wall plate and adopting a vacuum heat insulation structure.

[0015] Optionally, the closed cabin is provided with an airtight door and a dimmable observation window; the airtight door adopts a double-door interlocking structure; and the dimmable observation window is used for adjusting the spectrum in the closed cabin and / or displaying the environment monitoring parameter in response to an operation instruction.

[0016] Based on the above technical solutions, the present application has the following technical effects:

[0017] Firstly, the independent and controllable seedling hardening space is formed by arranging the closed cabin, so as to reduce the loss of heat in the cabin;

[0018] Secondly, by integrating an environmental control system into a sealed chamber, parameters such as light, CO2 concentration, temperature, and humidity within the chamber are adaptively adjusted based on gradient-changing parameter control curves. This optimizes the dynamic induction process of tissue culture seedlings from the laboratory environment to the natural environment, thereby improving the survival rate and quality of the seedlings.

[0019] Third, the seedling hardening process constrains the control curves of parameters such as light, CO2 concentration, temperature and humidity. The entire process requires no human intervention, has a high degree of standardization, low skill requirements, and can allow one person to manage multiple devices, saving labor costs. At the same time, during the seedling hardening process, the preset parameter control curves are dynamically optimized by monitoring the crop's transpiration rate, ensuring the safety and optimality of the seedling hardening and domestication process, and demonstrating a high degree of intelligence.

[0020] It should be understood that the description in this section is not intended to identify key or essential features of the embodiments of the present invention, nor is it intended to limit the scope of the invention. Other features of the invention will become readily apparent from the following description. Attached Figure Description

[0021] To more clearly illustrate the technical solutions in the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0022] Figure 1 A schematic diagram of an adaptive hardening and acclimatization chamber for tissue culture seedlings based on light quality regulation and CO2 gradient induction, provided for an embodiment of the present invention;

[0023] Figure 2 This is a schematic diagram of the structure of an adjustment module provided in an embodiment of the present invention;

[0024] Figure 3 This is a schematic diagram of a lamp panel array structure for a multi-band light source provided in an embodiment of the present invention;

[0025] Figure 4 A perspective view of a sealed chamber provided in an embodiment of the present invention;

[0026] Figure 5 This is a schematic diagram of a double-layer thermal insulation structure provided in an embodiment of the present invention. Detailed Implementation

[0027] To enable those skilled in the art to better understand the present invention, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of the present invention.

[0028] It should be noted that the terms "first," "second," etc., in the specification, claims, and accompanying drawings of this invention are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be interchanged where appropriate so that the embodiments of the invention described herein can be implemented in orders other than those illustrated or described herein. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion; for example, a process, method, system, product, or apparatus that comprises a series of steps or units is not necessarily limited to those steps or units explicitly listed, but may include other steps or units not explicitly listed or inherent to such processes, methods, products, or apparatus.

[0029] Figure 1 This is a schematic diagram of an adaptive hardening and acclimatization chamber for tissue culture seedlings based on light quality regulation and CO2 gradient induction, provided in an embodiment of the present invention. This embodiment can be applied to the hardening and acclimatization scenario of tissue culture seedlings of plants that are both medicinal and edible before transplanting.

[0030] like Figure 1 As shown, the tissue culture seedling adaptive acclimatization chamber based on light quality regulation and CO2 gradient induction of the present invention includes: a sealed chamber 1, and an environmental control system 2 installed inside the sealed chamber 1. In this embodiment, the environmental control system 2 is mainly used to regulate four key environmental parameters: light intensity (light intensity, light quality, and photoperiod), CO2 concentration, humidity, and temperature.

[0031] Optionally, the sealed chamber can adopt a cuboid structure, or a cylindrical or domed design to optimize the internal airflow. The sealed chamber can be a single chamber or a multi-chamber series structure, facilitating the formation of a continuous seedling hardening production line. It should be noted that in a multi-chamber series structure, the environmental parameters of different chambers can be adjusted independently. In this embodiment, a loading platform and an environmental control system 2 can be installed inside the sealed chamber 1. The loading platform is used to place tissue culture seedlings and the seedling trays and substrate required for tissue culture seedling cultivation.

[0032] like Figure 1As shown, the environmental control system 2 of the present invention includes: a control module 10, and a monitoring module 20 and an adjustment module 30 respectively connected to the control module 10; the control module 10 is used to store seedling hardening programs corresponding to different crops, adjust the adjustment module 30 according to the environmental monitoring parameters provided by the monitoring module 20 and the parameters recorded in the seedling hardening program, dynamically adjust the acclimatization environment in the sealed chamber 1, and correct the change gradient of the parameter adjustment curve according to the transpiration rate provided by the monitoring module 20.

[0033] The environmental monitoring parameters of this invention can be understood as parameters characterizing the real state of the simulated environment within the acclimatization chamber. In this embodiment, environmental monitoring parameters within the sealed chamber 1 can be collected by sensors installed at different locations. Typically, environmental monitoring parameters include, but are not limited to, measured values ​​of: illumination (light intensity, light quality, and photoperiod), CO2 concentration, humidity, and temperature.

[0034] The seedling hardening program of this invention can be understood as a program used to constrain the environmental conditions of different crops (e.g., Dendrobium officinale), different growth patterns (e.g., high polysaccharide pattern), and different acclimatization stages (including but not limited to the adaptation period, growth period, and induction period). Specifically, each seedling hardening program stores one or more parameter control curves. These parameter control curves define the target values ​​of light (intensity, light quality, period), CO2 concentration, temperature, and humidity at each moment throughout the entire seedling hardening cycle (e.g., 10 days), as well as the changes of these target values ​​over time. Specific parameters in the seedling hardening program, such as target values ​​and gradients, can be configured through a human-machine interface connected to the control module 10.

[0035] The parameter control curves of this invention include, but are not limited to: a light control curve, used at least to record the dynamic changes in light quality ratio, light intensity, and photoperiod required by crops at different acclimatization stages; a CO2 control curve, used at least to record the dynamic changes in the target CO2 concentration required by crops at different acclimatization stages; a humidity control curve, used at least to record the dynamic changes in the target humidity value required by crops at different acclimatization stages; and a temperature control curve, used at least to record the dynamic changes in the target temperature value required by crops at different acclimatization stages.

[0036] Specifically, before starting the acclimatization chamber, the rooted tissue culture seedlings are placed in customized seedling trays on the platform inside the sealed chamber 1. The operator then imports the preset acclimatization program into the control module 10. After the door of the sealed chamber 1 is closed, the control module 10 begins executing the acclimatization program. Based on the dynamic target values ​​and gradient changes of each environmental parameter corresponding to the light control curve, CO2 control curve, humidity control curve, and temperature control curve recorded in the acclimatization program throughout the entire acclimatization cycle (e.g., 10 days), the environmental parameters are controlled to gradually transition from laboratory tissue culture conditions to near-natural environmental conditions. During the execution of the acclimatization program, a PID control signal is output to the adjustment module 30 to ensure that the environmental parameters accurately follow the parameter control curves recorded in the acclimatization program. At the same time, the gradient of the humidity control curve is corrected according to the transpiration rate of the crop until the entire acclimatization program is completed, thus completing the acclimatization of the tissue culture seedlings.

[0037] Therefore, the technical solution of this invention, by setting up a sealed chamber to form an independent and controllable seedling hardening and acclimatization space, reduces the loss of heat and cold inside the chamber; by integrating an environmental control system within the sealed chamber, the system adaptively adjusts parameters such as light, CO2 concentration, temperature, and humidity within the chamber based on gradient-changing parameter control curves, optimizing the dynamic induction process of tissue culture seedlings from the laboratory environment to the natural environment, thereby improving the survival rate and quality of seedlings; by constraining the control curves of parameters such as light, CO2 concentration, temperature, and humidity through the seedling hardening program, the entire process requires no manual intervention, has a high degree of standardization, low skill requirements, and can allow one person to manage multiple devices, saving labor costs; at the same time, during the seedling hardening process, the preset parameter control curves are dynamically optimized by monitoring the transpiration rate of the crop, ensuring the safety and optimality of the seedling hardening and acclimatization process, and demonstrating a high degree of intelligence.

[0038] Figure 2 This is a schematic diagram of the structure of an adjustment module provided in an embodiment of the present invention.

[0039] See Figure 2 As shown, the adjustment module 30 of the present invention includes a multi-band light source 310 for adjusting the illumination inside the sealed chamber 1. In this embodiment, the multi-band light source 310 is provided with multiple light-emitting units of different bands, and each light-emitting unit is correspondingly arranged with a driver. The control module 10 is configured to: determine the drive control signal of any driver according to the light quality ratio, light intensity, and light cycle, and perform closed-loop adjustment of the drive control signal according to the measured light value in the environmental monitoring parameters.

[0040] Figure 3 This is a schematic diagram of a lamp panel array structure for a multi-band light source provided in an embodiment of the present invention. Figure 3As shown, the multi-band light source 310 of the present invention is formed by combining an ultraviolet light band (e.g., 315 to 400 nm band) light-emitting unit 310UV, a blue light band (e.g., 450 to 470 nm band) light-emitting unit 310B, a red light band (e.g., 655 to 665 nm band) light-emitting unit 310R, a far-infrared light band (e.g., 725 to 735 nm band) light-emitting unit 310FR, and a full-spectrum light-emitting unit 310W; the light source type of the multi-band light source adopts any one or a combination of LED light source, OLED light source, and laser diode.

[0041] Specifically, the multi-band light source 310 can be installed on the ceiling inside the cabin. The multi-band light source 310 is formed by assembling several modular LED light source panels. (See reference...) Figure 3 As shown, each LED light source board has five independent light-emitting units evenly distributed: the ultraviolet light-emitting unit 310UV can use an LED with a wavelength of 385nm and a single power of 1W; the blue light-emitting unit 310B can use an LED with a wavelength of 450nm and a single power of 3W; the red light-emitting unit 310R can use an LED with a wavelength of 660nm and a single power of 3W; the far-infrared light-emitting unit 310FR can use an LED with a wavelength of 730nm and a single power of 2W; and the full-spectrum light-emitting unit 310W can use an LED with a color temperature of 4000K and a single power of 2W. Each LED is driven by an independent constant current driver. The operation of the multi-band light source 310 is as follows: the control module 10 controls each constant current driver through a drive control signal (e.g., a pulse width modulation signal PWM), thereby independently and precisely adjusting the luminous intensity of each LED (e.g., adjustable from 0-100%). By setting the seedling hardening program for different crops, arbitrary light quality ratios (such as a low light quality ratio of red to far-red light (R:FR) can promote seedling elongation, while a high light quality ratio of red to blue light (R:B) can promote robust seedling growth), arbitrary light intensity (0-500 µmol / m² / s), and photoperiod can be combined. Furthermore, timed short-term stress stimulation with ultraviolet A rays (UV-A) can be achieved to activate secondary metabolic pathways.

[0042] See also Figure 2As shown, the adjustment module 30 of the present invention further includes a CO2 gas adjustment submodule 320, used to adjust the CO2 concentration within the sealed chamber 1. The CO2 gas adjustment submodule 320 includes a CO2 storage tank 321 and a CO2 injection pipeline 322, the CO2 injection pipeline 322 being connected to the CO2 storage tank 321 via a flow controller 323. The control module 10 is configured to: during the illumination phase, compare the target CO2 concentration with the measured CO2 concentration value in the environmental monitoring parameters, and determine the preset flow rate value of the flow controller based on the comparison result; the flow controller is configured to: adjust the CO2 gas flow rate through the CO2 injection pipeline according to the preset flow rate value.

[0043] In this embodiment, the flow controller 323 can be set to a range of 0-5 L / min and an accuracy of ±1% of full scale.

[0044] In some optional embodiments, the CO2 injection line 322 adopts an annular diffusion tube structure; the diffusion tube is provided with micropores, which are evenly distributed in the bottom space of the sealed chamber 1.

[0045] In some optional embodiments, the CO2 gas conditioning submodule further includes a circulating fan for accelerating gas diffusion.

[0046] Specifically, during the illumination phase, the control module 10 compares the target CO2 concentration with the measured CO2 concentration in the environmental monitoring parameters, calculates the preset flow rate of the flow controller based on the deviation between the target CO2 concentration and the measured CO2 concentration, and sends this preset flow rate to the flow controller 323. The flow controller 323 adjusts the CO2 gas flow rate through the CO2 injection pipeline according to this preset flow rate. The CO2 gas is evenly released into the chamber through micropores on the diffusion tube and rapidly mixed by a circulating fan, causing the CO2 concentration in the chamber to quickly reach and stabilize at the target CO2 concentration. Simultaneously, by utilizing the synergistic effect of specific light quality and CO2 during the photoperiod, the photomorphogenesis and secondary metabolism of plants are actively regulated, which can increase the content of functional components in medicinal and edible plants and improve seedling quality.

[0047] See Figure 2As shown, the adjustment module 30 of the present invention further includes a humidity adjustment submodule 330 for adjusting the air humidity inside the sealed chamber 1. The humidity adjustment submodule 330 includes a humidification unit 331 and a cooling / dehumidification unit 332 arranged in parallel. The humidification unit 331 can form atomized particles using at least one of ultrasonic humidification, high-pressure micro-mist, or wet film evaporation, with the diameter of the atomized particles being less than 5 μm. The cooling / dehumidification unit 332 is disposed on the side wall of the sealed chamber 1, with its cold end facing the interior space of the sealed chamber 1 and its hot end facing the exterior space of the sealed chamber 1. The hot end of the cooling / dehumidification unit 332 is equipped with heat dissipation fins and a fan. The control module 10 is configured to compare the target humidity value with the measured humidity value in the environmental monitoring parameters, and control the humidification unit to perform humidification or, based on the comparison result, control the cooling / dehumidification unit to perform dehumidification.

[0048] Specifically, the control module 10 compares the target humidity value with the measured humidity value in the environmental monitoring parameters. If the target humidity value is higher than the measured humidity value, it controls the humidification unit to generate fine water mist to increase the humidity in the acclimatization chamber. If the target humidity value is lower than the measured humidity value, it controls the cooling and dehumidification unit 332 to start. The cold end of the cooling and dehumidification unit 332 condenses water vapor in the air inside the chamber into water and discharges it, reducing the humidity in the acclimatization chamber. Thus, by setting up a parallel technical solution of ultrasonic humidification and semiconductor dehumidification, the humidity can be adjusted within the range of 40% to 95%, improving the humidity adjustment accuracy to ±3%RH, and solving the problem of wide-range, rapid, and high-precision humidity adjustment.

[0049] In some alternative embodiments, the humidity control submodule 330 may also employ compressor-based dehumidification or rotary dehumidification.

[0050] See Figure 2 As shown, the adjustment module 30 of the present invention further includes a temperature control unit 340 for adjusting the temperature inside the sealed chamber 1. Optionally, the temperature control unit 340 may be a semiconductor temperature control unit, which is disposed on the side wall of the sealed chamber 1, and both ends of the semiconductor temperature control unit are connected to heat sinks; the control module 10 is configured to compare the target temperature value with the measured temperature value in the environmental monitoring parameters, and determine the current direction and current magnitude of the driving current of the semiconductor temperature control unit based on the comparison result.

[0051] Specifically, the semiconductor temperature control unit is designed based on the Peltier effect, with large aluminum heat sinks connected to both the cold and hot ends. A powerful fan is installed on the external heat sink. The semiconductor temperature control unit operates as follows: The control module 10 compares the target temperature value with the measured temperature value from the environmental monitoring parameters. If the target temperature value is lower than the measured value, the operating mode is switched to cooling mode by changing the direction of the DC current input to the semiconductor temperature control unit. Simultaneously, the driving current is adjusted according to the temperature difference between the target and measured values ​​to regulate the cooling power. If the target temperature value is higher than the measured value, the operating mode is switched to heating mode by changing the direction of the DC current input to the semiconductor temperature control unit. The driving current is adjusted according to the temperature difference between the target and measured values ​​to regulate the heating power. Precise temperature control is achieved through a PID algorithm, stabilizing the temperature at the target value. By setting up the semiconductor temperature control unit, a vibration-free and precisely temperature-controlled environment is provided for the acclimatization chamber.

[0052] In some alternative embodiments, the temperature control unit 340 may also employ a heat pump system, the specific regulation process of which is similar to that of the semiconductor temperature control unit and will not be described in detail here.

[0053] The monitoring module 20 of the present invention includes a transpiration rate monitoring unit; the control module 10 is configured to: determine the real-time transpiration rate of the crop based on the measured value provided by the transpiration rate monitoring unit, and correct the change gradient of the parameter control curve when the real-time transpiration rate exceeds a preset rate threshold.

[0054] In some optional embodiments, the transpiration rate monitoring unit can employ a weighing sensor, mounted on the base of the chamber. Specifically, taking the transpiration rate acquisition by weighing as an example, a loading platform can be set up, such as a perforated 7075 aluminum alloy platform, which is fixed to the weighing sensor by four support rods. The weighing sensor is installed on the base at the bottom of the chamber. Rooted tissue culture seedlings are placed in customized seedling trays on the loading platform. The loading platform, the tissue culture seedlings, the seedling trays, and the substrate are treated as a whole, and the weighing sensor acquires the actual weight of this whole in real time. This actual weight is transmitted to the control module 10 in real time. The control module 10 calculates the rate of decrease of the actual weight and converts the rate of decrease of the actual weight into the real-time transpiration rate of the plant population using the relationship between weight and transpiration rate. It should be noted that this real-time transpiration rate is the result measured by the weighing sensor, taking into account factors such as the evaporation of soil surface water and weight changes caused by the plant's own growth and metabolism. If the real-time transpiration rate exceeds the preset transpiration threshold in the seedling hardening program, it indicates that the humidity in the chamber is decreasing too quickly. The control module 10 automatically adjusts the humidity control curve, reducing the descent gradient of the humidity control curve until the transpiration rate returns to the normal range, thus achieving adaptive correction of the control curve.

[0055] In some alternative embodiments, the transpiration rate monitoring unit may be implemented using non-weighing methods such as leaf surface humidity sensors or thermal imagers.

[0056] Figure 4 This is a perspective view of a sealed chamber provided in an embodiment of the present invention. See also... Figure 4 As shown, the sealed chamber 1 is equipped with an airtight door 110 and a dimming observation window 120; the airtight door 110 adopts a double-door interlocking structure; the dimming observation window 120 is used to adjust the spectrum and / or display environmental monitoring parameters inside the sealed chamber 1 in response to operating commands. Specifically, a sliding airtight door with a double-door interlocking structure and an electrically dimmable liquid crystal glass observation window can be provided at the front of the sealed chamber 1. By setting the airtight door and the dimming observation window, the stability of the internal environment of the acclimatization chamber is ensured, and at the same time, the internal light cycle is not affected during observation.

[0057] Optionally, the sealed chamber 1 of the present invention may adopt a double-layer heat insulation structure.

[0058] For example, Figure 5 This is a schematic diagram of a double-layer thermal insulation structure provided in an embodiment of the present invention. See also... Figure 5 As shown, the double-layer heat insulation structure of the present invention includes: an inner wall panel 101, disposed on the side facing the interior space of the sealed chamber 1, and made of reflective material; an outer wall panel 102, disposed on the side facing the exterior space of the sealed chamber 1, and made of engineering plastic; and a heat insulation layer 103, disposed between the inner wall panel 101 and the outer wall panel 102, and made of vacuum heat insulation structure.

[0059] For example, such as Figure 4 As shown, the sealed chamber 1 adopts a cuboid structure, consisting of a frame and panels. The panels employ a double-layer insulation structure: the inner wall panel is made of 1.0mm thick mirror-finished anodized aluminum with a light reflectivity ≥95%; the outer wall panel is made of 1.5mm thick engineering plastic (Acrylonitrile-Butadiene-Styrene copolymer, abbreviated as ABS); and a 30mm thick vacuum insulation panel (VIP) is filled in between. By setting up a double-layer insulation structure, the heat exchange between the inside and outside of the chamber is greatly reduced, lowering the energy consumption for environmental control in the acclimatization chamber. For example, compared with a traditional constant-temperature chamber that continuously runs a compressor, the overall energy consumption of this system is reduced by approximately 30% for the same volume. The highly reflective inner wall ensures uniform light distribution within the chamber, preventing excessive plant growth.

[0060] For example, Table 1 shows a data table corresponding to a set of parameter control curves for Dendrobium officinale.

[0061] Stage Time Light (quality / intensity / cycle) [CO2 concentration] Temperature Humidity Principle and purpose of programming Adaptation period Day 1-3 Light quality: R:B = 2:1 (main red light for germination) Light intensity: 100 μmol / m² / s Photoperiod: 12h / 12h 600 ppm 25°C ±0.5 85% ± 3% High humidity, weak light, supplemented with CO2, simulate tissue culture environment, relieve domestication stress, and start photosynthesis smoothly. Growth period Day 4-7 Light quality: R:B = 1:1 (balanced light for seedling) Light intensity: increase 20 μmol / m² / s per day, up to 180 μmol / m² / s on day 7 Photoperiod: 14h / 10h 1000 ppm 25°C ±0.5 Decrease 5% per day, up to 65% on day 7 Enhanced light intensity, extended light, applied CO2 fertilization, greatly improved photosynthetic efficiency, promoted biomass (dry weight) accumulation. At the same time, the gradient reduces humidity, promotes stomatal function development. Induction period Day 8-10 Light quality: add UV-A (385nm), 2h / day (midday) Light intensity: 200 μmol / m² / s Photoperiod: 12h / 12h 800 ppm Day-night temperature difference: day 28°C / night 22°C 60% ± 3 Core stage: UV-A as a mild stress signal, effectively activates plant defense mechanisms, significantly up-regulates polysaccharide synthesis pathway gene expression. Day-night temperature difference further stimulates secondary metabolism. Ultimately achieve quality improvement.

[0062] As shown in Table 1, the light intensity can be the total light intensity of each wavelength light source under a specific light quality. Taking the cultivation of Dendrobium officinale as an example, the seedling hardening program records the target values ​​of the parameters corresponding to the parameter control curves during the adaptation period, growth period, and induction period, as well as the gradient changes of each parameter at different stages. During the seedling hardening adaptation period, the control module 10 uses a PID algorithm to control the CO2 concentration in the chamber at 600 ppm, the temperature in the chamber at 25℃±0.5℃, the humidity in the chamber at 85%±3%, the light quality ratio R:B of the red light and blue light in the chamber at R:B=2:1, and the light intensity in the chamber at 100 μmol / m² / s. The photocycle is 12 hours of irradiation followed by 12 hours of extinguishing, in order to maintain high humidity, low light, and supplemental CO2 conditions in the acclimatization chamber, simulate the tissue culture environment, relieve acclimatization stress, and smoothly start photosynthesis. During the growth period, the gradient change of the humidity regulation curve satisfies the following: decrease by 5% daily, reaching 65% on the 7th day; the gradient change of light intensity satisfies the following: increase by 20 μmol / m² / s daily, reaching 180 μmol / m² / s on the 7th day.

[0063] As shown in Table 1, the technical solution of this application avoids the physiological stress caused by environmental mutations to seedlings through smooth gradient changes and PID control mechanism, thereby improving the seedling survival rate; through the synergistic regulation program of light quality and CO2, it effectively activates the plant's defense mechanism, significantly upregulates the gene expression of polysaccharide synthesis pathway, increases the effective components of "medicinal and edible homology" products, and improves seedling quality.

[0064] It should be understood that the various forms of processes shown above can be used, with steps reordered, added, or deleted. For example, the steps described in this invention can be executed in parallel, sequentially, or in different orders, as long as the desired result of the technical solution of this invention can be achieved, and this is not limited herein.

[0065] The specific embodiments described above do not constitute a limitation on the scope of protection of this invention. Those skilled in the art should understand that various modifications, combinations, sub-combinations, and substitutions can be made according to design requirements and other factors. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this invention should be included within the scope of protection of this invention.

Claims

1. A tissue culture seedling adaptive hardening and acclimatization chamber based on light quality regulation and CO2 gradient induction, characterized in that, include: A sealed chamber, and an environmental control system installed inside the sealed chamber; The environmental control system includes: a control module, and a monitoring module and an adjustment module respectively connected to the control module; The control module is used to store the hardening-off program corresponding to different crops, and to control the adjustment module to dynamically adjust the acclimatization environment in the sealed chamber according to the environmental monitoring parameters provided by the monitoring module and the parameter adjustment curve recorded in the hardening-off program, and to correct the change gradient of the parameter adjustment curve according to the transpiration rate provided by the monitoring module. The parameter adjustment curve includes: The light regulation curve is used to record at least the dynamic changes in the light quality ratio, light intensity, and photoperiod required by the crop at different domestication stages; CO2 regulation curves are used at least to record the dynamic changes in the target CO2 concentration required by the crop at different domestication stages; Humidity control curves are used to record at least the dynamic changes in the target humidity values ​​required by the crop at different acclimatization stages. Temperature regulation curves are used to record at least the dynamic changes in the target temperature values ​​required by the crop at different stages of domestication.

2. The tissue culture seedling adaptive hardening-off chamber based on light quality regulation and CO2 gradient induction as described in claim 1, characterized in that, The adjustment module includes: a multi-band light source, wherein the multi-band light source is provided with multiple light-emitting units of different bands, and each light-emitting unit is configured in a one-to-one correspondence with a driver; The control module is configured to: determine the drive control signal of any of the drivers based on the light quality ratio, the light intensity and the light cycle, and perform closed-loop adjustment of the drive control signal based on the measured light value in the environmental monitoring parameters.

3. The tissue culture seedling adaptive hardening and acclimatization chamber based on light quality regulation and CO2 gradient induction as described in claim 2, is characterized in that, The multi-band light source is formed by combining ultraviolet light band light-emitting units, blue light band light-emitting units, red light band light-emitting units, far-infrared light band light-emitting units, and full-spectrum light-emitting units; The multi-band light source can be any one or a combination of LED light sources, OLED light sources, and laser diodes.

4. The tissue culture seedling adaptive hardening and acclimatization chamber based on light quality regulation and CO2 gradient induction as described in claim 1, characterized in that, The regulating module includes a CO2 storage tank and a CO2 injection pipeline, wherein the CO2 injection pipeline is connected to the CO2 storage tank via a flow controller; The control module is configured to: during the illumination phase, compare the target CO2 concentration with the measured CO2 concentration in the environmental monitoring parameters, and determine the preset flow rate of the flow controller based on the comparison result; The flow controller is configured to adjust the flow rate of CO2 gas flowing through the CO2 injection pipeline according to the preset flow rate value.

5. The tissue culture seedling adaptive hardening and acclimatization chamber based on light quality regulation and CO2 gradient induction according to claim 4, characterized in that, The CO2 injection pipeline adopts a ring-shaped dispersion tube structure; The diffusion tube is provided with micropores, which are evenly distributed in the bottom space of the sealed chamber.

6. The tissue culture seedling adaptive hardening and acclimatization chamber based on light quality regulation and CO2 gradient induction according to claim 1, characterized in that, The adjustment module includes: a humidification unit and a cooling and dehumidification unit connected in parallel; The humidification unit uses at least one of ultrasonic humidification, high-pressure micro-mist, or wet film evaporation to form atomized particles, and the diameter of the atomized particles is less than 5 μm; The refrigeration and dehumidification unit is disposed on the side wall of the sealed chamber, with the cold end of the refrigeration and dehumidification unit facing the interior space of the sealed chamber and the hot end of the refrigeration and dehumidification unit disposed in the exterior space of the sealed chamber, and the hot end of the refrigeration and dehumidification unit is provided with heat dissipation fins and a fan. The control module is configured to: compare the target humidity value with the measured humidity value in the environmental monitoring parameters, and control the humidification unit to perform humidification or control the refrigeration and dehumidification unit to perform dehumidification based on the comparison result.

7. The tissue culture seedling adaptive hardening and acclimatization chamber based on light quality regulation and CO2 gradient induction according to claim 1, characterized in that, The adjustment module includes: a temperature control unit; The temperature control unit is located on the side wall of the sealed chamber, and both ends of the temperature control unit are connected to heat sinks; The control module is configured to compare the target temperature value with the measured temperature value in the environmental monitoring parameters, and determine the current direction and magnitude of the driving current of the temperature control unit based on the comparison result.

8. The tissue culture seedling adaptive hardening and acclimatization chamber based on light quality regulation and CO2 gradient induction according to claim 1, characterized in that, The monitoring module includes a transpiration rate monitoring unit; The control module is configured to: determine the real-time transpiration rate of the crop based on the measured value provided by the transpiration rate monitoring unit, and correct the gradient of the parameter control curve when the real-time transpiration rate exceeds a preset rate threshold.

9. The tissue culture seedling adaptive hardening and acclimatization chamber based on light quality regulation and CO2 gradient induction according to any one of claims 1-8, characterized in that, The sealed cabin adopts a double-layer thermal insulation structure: The double-layer thermal insulation structure includes: The inner wall panel, located on the side facing the interior space of the sealed cabin, is made of reflective material. The outer wall panel, located on the side facing the external space of the sealed cabin, is made of engineering plastic. A heat insulation layer is disposed between the inner wall panel and the outer wall panel, and adopts a vacuum heat insulation structure.

10. The tissue culture seedling adaptive hardening and acclimatization chamber based on light quality regulation and gradient induction according to any one of claims 1-8, characterized in that, The sealed cabin is equipped with an airtight door and a dimming observation window; The airtight door adopts a double-door interlocking structure; The dimming observation window is used to adjust the spectrum inside the sealed chamber and / or display the environmental monitoring parameters in response to operating commands.

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