Cell culture cabin suitable for small-size equipment and control system of cell culture cabin

Abstract

The invention relates to a cell culture cabin suitable for small-size equipment and a control system of the cell culture cabin in the biomedical equipment technology, and the cell culture cabin comprises an environment cabin in which an environment cavity is formed; the culture cabin is arranged in the environment cavity and is used for fixing a culture dish; the temperature detection and regulation system is connected to the environment cavity and is used for detecting, regulating and controlling the temperature of the environment cavity; the humidity detection and regulation system is connected to the environment cavity and is used for detecting, regulating and controlling the humidity of the environment cavity; and the carbon dioxide detection and regulation system is connected to the environment cavity and is used for detecting and regulating the carbon dioxide concentration of the environment cavity. According to the cell culture cabin suitable for the small-size equipment and the control system of the cell culture cabin, parameters such as temperature, humidity and carbon dioxide concentration can be accurately regulated and controlled, and stable and appropriate growth conditions are provided for cell culture.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biomedical equipment, in particular to a cell culture cabin suitable for small volume equipment and a control system thereof. BACKGROUND

[0002] Cell culture refers to the process of taking cells out of animals or plants and then growing them in a suitable artificial environment. Cell culture is a core means of modern biology, medicine and biotechnology research, which needs to simulate the living environment of cells in vivo. Carbon dioxide incubators can provide an environment with certain temperature, humidity and carbon dioxide concentration, providing stable and suitable growth conditions for cell culture. For example, in cancer research, researchers control cell environment and other factors to complete cell growth and proliferation and drug response, thereby providing an important basis for anticancer drugs.

[0003] However, the existing cell culture incubator still has the problems of single parameter regulation, low precision, weak anti-interference ability, high pollution risk and the like, and is mainly suitable for basic culture scenes with low environmental requirements, and has many limitations in function, stability and operation convenience, and is difficult to meet the needs of complex cell culture or high-precision experiments. SUMMARY

[0004] The main purpose of the present application is to provide a cell culture cabin suitable for small volume equipment and a control system thereof, which aims to solve at least one of the above technical problems.

[0005] To achieve the above purpose, the present application provides a cell culture cabin suitable for small volume equipment, comprising: an environment cabin, which forms an environment cavity inside; a culture cabin, which is arranged in the environment cavity and is used for fixing a culture dish; a temperature detection and regulation system, which is connected to the environment cavity and is used for detecting and regulating the temperature of the environment cavity; a humidity detection and regulation system, which is connected to the environment cavity and is used for detecting and regulating the humidity of the environment cavity; and a carbon dioxide detection and regulation system, which is connected to the environment cavity and is used for detecting and regulating the carbon dioxide concentration of the environment cavity.

[0006] By using the cell culture cabin in the present application, the internal space of the environment cabin is formed into an environment cavity required for cell growth, a culture cabin for fixing a culture dish is arranged in the environment cavity, and then the temperature detection and regulation system, the humidity detection and regulation system and the carbon dioxide detection and regulation system accurately detect the environment parameters of the environment cavity, so that the temperature detection and regulation system, the humidity detection and regulation system and the carbon dioxide detection and regulation system can accurately regulate the environment of the environment cavity based on the detection results to achieve the required temperature, humidity and carbon dioxide concentration, thereby providing stable and suitable growth conditions for cell culture.

[0007] In some embodiments of the present application, the temperature detection and adjustment system comprises: a multi-point temperature sensor module comprising multiple groups of temperature sensors respectively arranged at multiple points of the environmental chamber; and a distributed temperature adjustment module comprising multiple groups of semiconductor refrigeration plates and heating films respectively arranged at multiple positions of the environmental chamber.

[0008] In some embodiments of the present application, the carbon dioxide detection and adjustment system comprises: a carbon dioxide measurement module comprising an infrared sensor and / or a thermal conductivity sensor; and a gas supply module comprising an oxygen gas tank, a carbon dioxide gas tank, a gas mixing structure, a solenoid valve and a mass flow meter, wherein the oxygen gas tank and the carbon dioxide gas tank are connected to the environmental chamber in sequence via the gas mixing structure, the solenoid valve and the mass flow meter.

[0009] In some embodiments of the present application, the gas supply module is connected to the bottom of the environmental chamber, and an air flow plate is arranged at the top of the environmental chamber to form a vertical circulating air flow.

[0010] In some embodiments of the present application, a sterile air filtration system is arranged between the gas supply module and the environmental chamber.

[0011] In some embodiments of the present application, the cell culture cabin further comprises: a deep ultraviolet LED sterilization module arranged in the environmental chamber for sterilizing and disinfecting the culture environment.

[0012] In some embodiments of the present application, the environmental cabin comprises: an environmental cabin lower shell; an environmental cabin upper shell mounted on the top of the environmental cabin lower shell to form the environmental chamber with the environmental cabin lower shell; and a sealing gasket arranged between the environmental cabin lower shell and the environmental cabin upper shell to seal the environmental chamber.

[0013] In some embodiments of the present application, the environmental cabin further comprises: a transparent glass, and a mounting opening for mounting the transparent glass is arranged at the top of the environmental cabin upper shell corresponding to the environmental chamber; and the transparent glass is an electric heating glass.

[0014] In some embodiments of the present application, the culture cabin comprises: a culture cabin lower shell; and a culture cabin upper shell mounted on the top of the culture cabin lower shell to form a space for fixing a culture dish between the culture cabin lower shell and the culture cabin upper shell.

[0015] To achieve the above-mentioned purposes, the present application provides a control system comprising the above-mentioned cell culture cabin, a detection module and a main control module. The detection module can receive detection parameters of the temperature detection and adjustment system, the humidity detection and adjustment system and the carbon dioxide detection and adjustment system and send them to the main control module. The main control module can control the temperature detection and adjustment system, the humidity detection and adjustment system and the carbon dioxide detection and adjustment system to regulate the temperature, humidity and carbon dioxide concentration of the environmental chamber according to the detection parameters. BRIEF DESCRIPTION OF DRAWINGS

[0016] Various other advantages and benefits will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiments. The detailed description is made with reference to the accompanying drawings.

[0017] Figure 1 is a schematic diagram of the overall structure of the cell culture chamber of the present application;

[0018] Figure 2 is a schematic diagram of the cell culture chamber of the present application in an open state;

[0019] Figure 3 is a schematic diagram of the temperature detection and regulation system of the present application;

[0020] Figure 4 is a schematic diagram of the carbon dioxide detection and regulation system of the present application;

[0021] Figure 5 is a schematic diagram of the humidity detection and regulation system of the present application;

[0022] Figure 6 is a schematic diagram of the temperature regulation of the cell culture chamber of the present application;

[0023] Figure 7 is a schematic diagram of the temperature control of the cell culture chamber of the present application;

[0024] Figure 8 is a schematic diagram of the carbon dioxide concentration regulation of the cell culture chamber of the present application;

[0025] Figure 9 is a schematic diagram of the carbon dioxide concentration control of the cell culture chamber of the present application.

[0026] The various elements in the drawings are designated by the same reference numbers. In the drawings: 1. lower shell of the environment chamber; 2. upper shell of the environment chamber; 3. transparent glass; 4. lower shell of the culture chamber; 5. upper shell of the culture chamber; 6. temperature sensor; 7. semiconductor refrigeration sheet; 8. heating film; 9. carbon dioxide sensor; 10. oxygen gas tank; 11. carbon dioxide gas tank; 12. gas mixing structure; 13. electromagnetic valve; 14. mass flow meter; 15. sterile air filtration system; 16. lock catch; 17. bayonet; 18. gasket; 190. humidity sensor; 191. humidity regulation module; 20. display screen; 21. USB port; 22. power switch; 23. culture dish; 24. probe. DETAILED DESCRIPTION

[0027] It should be noted that the described embodiments are merely some of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative labor fall within the scope of protection of the present application.

[0028] The following description refers to the accompanying drawings. In the following description, same numbers in different drawings represent the same or similar elements unless otherwise represented. The implementations described in the following exemplary embodiments are not meant to represent all implementations consistent with the present application. Rather, they are merely examples of apparatuses and methods consistent with some aspects of the present application as detailed in the appended claims.

[0029] In the description of the present application, it should be understood that the terms "first", "second", etc. are only for the purpose of description, and cannot be understood as indicating or implying relative importance. For those of ordinary skill in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances. In addition, in the description of the present application, "multiple" means two or more, unless otherwise stated. The association between the associated objects is described, which means that there can be three relationships, for example, A and / or B can represent the following three cases: A exists alone, A and B exist together, and B exists alone. The character " / " generally represents that the associated objects before and after are in an "or" relationship.

[0030] The cell incubator is a key equipment for cell culture, but the existing cell incubator still has the problems of single parameter regulation, low precision, weak anti-interference ability, high pollution risk, etc., and is mainly suitable for basic culture scenes with low environmental requirements. There are many limitations in function, stability and operation convenience, which is difficult to meet the needs of complex cell culture or high-precision experiments.

[0031] For example, cell culture needs to achieve accurate temperature control of ±0.1℃, but the existing incubator has a significant temperature difference (local fluctuation > ±0.3℃) in a limited space, resulting in poor repeatability of experimental results. Traditional equipment relies on forced air circulation and large volume heat capacity design, and it is difficult to balance fast thermal response and uniformity. The temperature recovery time is generally more than 5 minutes. The traditional incubator is bulky (usually ≥100L), which is difficult to adapt to laboratory space limited scenarios (such as mobile laboratory, high-throughput screening platform, live cell workstation). Existing miniaturization attempts are limited by low heat dissipation efficiency, uneven gas mixing and other problems, resulting in a significant decline in environmental parameter control performance. In a limited volume (such as ≤30cm×30cm×30cm), the traditional design cannot balance the layout requirements of air flow distribution, thermal control module and environmental cavity, and is prone to condensate accumulation, local overheating / overcooling and other defects, which limits the integration capability of high-density multi-well culture dishes.

[0032] Therefore, the application provides a cell culture cabin suitable for small-volume equipment and a control system thereof, which can accurately control temperature, humidity, and carbon dioxide concentration, and provide stable and suitable growth conditions for cell culture.

[0033] The application discloses a cell culture cabin suitable for small-volume equipment, as shown in Figure 1 and Figure 2 The cell culture cabin comprises an environment cabin, a culture cabin, a temperature detection and regulation system, a humidity detection and regulation system, and a carbon dioxide detection and regulation system. The environment cabin forms an environment cavity. The culture cabin is arranged in the environment cavity and is used for fixing a culture dish 23. The temperature detection and regulation system, the humidity detection and regulation system, and the carbon dioxide detection and regulation system are connected to the environment cavity and are respectively used for detecting and regulating the temperature, the humidity, and the carbon dioxide concentration of the environment cavity.

[0034] In the application, the interior space of the environment cabin forms an environment cavity for cell growth. The culture cabin for fixing the culture dish 23 is arranged in the environment cavity. Then, the temperature detection and regulation system, the humidity detection and regulation system, and the carbon dioxide detection and regulation system accurately detect the environment parameters of the environment cavity, so that the temperature detection and regulation system, the humidity detection and regulation system, and the carbon dioxide detection and regulation system can accurately regulate the environment of the environment cavity to the required temperature, humidity, and carbon dioxide concentration based on the detection results, and provide stable and suitable growth conditions for cell culture.

[0035] In some embodiments of the application, as shown in Figure 3 The temperature detection and regulation system comprises a multi-point temperature sensor module and a distributed temperature regulation module. The multi-point temperature sensor module comprises a plurality of groups of temperature sensors 6 and is arranged at a plurality of points of the environment cavity. The distributed temperature regulation module comprises a plurality of groups of semiconductor refrigeration sheets 7 and heating films 8 and is arranged at a plurality of positions of the environment cavity.

[0036] The semiconductor refrigeration sheet 7 (TEC: thermoelectric cooler) can realize rapid reduction and accurate regulation of temperature and uniform absorption of heat through the Peltier effect. The heating film 8 adopts a planar design and can realize surface heating, can uniformly diffuse heat to the environment cavity, reduce local high-temperature or low-temperature zones, and can realize rapid increase and accurate regulation of temperature and uniform diffusion of heat.

[0037] In the present embodiment, by adopting multiple groups of temperature sensors 6, real-time monitoring of multiple temperature zone data can realize full-range measurement of the temperature in the environmental cavity. In combination with the distributed temperature regulation module, for temperature data of different temperature zones, multiple groups of semiconductor refrigerating sheets 7 and heating films 8 work cooperatively, for example, high-temperature and low-temperature temperature zones are respectively cooled by the semiconductor refrigerating sheets 7 and heated by the heating films 8, which can realize that the temperature uniformity error in the environmental cavity is greatly reduced, the rapid thermal response capability is greatly improved, the temperature uniformity error in the environmental cavity is as low as ≤±0.05℃, and the temperature recovery time is shortened to <3 minutes.

[0038] In some embodiments of the present application, the heating film 8 can adopt a PI heating film with a thickness ≤0.1mm attached to the inner wall of the environmental cavity.

[0039] Specifically, the PI heating film in the present application is made of metal (stainless steel / copper / iron-aluminum) as a heating base material and PI film as an insulating cover film. Through etching process, high-temperature compression and curing, an electric heating film with insulation, waterproof, light and soft is formed. The PI heating film has three layers, the first layer is a yellow flexible PI film, the second layer is a heating resistor, and the third layer is a PI film insulating layer. By applying different duty cycle PWM voltages to the heating resistor, the temperature of the PI heating film can be changed.

[0040] In the present application, the power consumption of the PI heating film is <20W, the power consumption of the semiconductor refrigerating sheet 7 is <15W, and the overall power consumption is reduced by 60% compared with traditional equipment.

[0041] In some embodiments of the present application, the multi-point temperature sensor module includes but is not limited to three, four or five groups of temperature sensors 6, preferably four groups of temperature sensors 6, which can monitor 4 temperature zone (heating node) data in real time.

[0042] In some embodiments of the present application, each group of temperature sensors 6 can include PT1000 and / or LMT70, preferably a combination of PT100 and LMT70, i.e. each group of temperature sensors 6 includes at least one PT100 sensor and one LMT70 sensor.

[0043] In the present embodiment, the LMT70 sensor and the PT100 sensor have an accuracy of ±0.05℃, the LMT70 sensor is placed beside the PT100 sensor as a backup sensor to ensure the accuracy of temperature acquisition, and in combination with multi-point acquisition, the temperature uniformity error in the environmental cavity is ensured to be ≤±0.05℃.

[0044] The PI heating film and the micro semiconductor refrigeration sheet can realize rapid temperature rise and fall, effectively ensure that the temperature recovery time is less than 3 minutes, meet the requirement of rapid temperature change in the cell culture process, are particularly important for researches which need to frequently adjust the culture conditions, can significantly shorten the experimental period, and improve the work efficiency.

[0045] In some embodiments of the present application, the PT100 sensor, the LMT70 sensor and the heating film can be detachably assembled with the environment cavity, so that they can be quickly disassembled and replaced, and the maintenance cost is reduced.

[0046] In some embodiments of the present application, as shown in Figure 4 The carbon dioxide detection and adjustment system includes a carbon dioxide measurement module and a gas supply module; the carbon dioxide measurement module includes a carbon dioxide sensor 9, which can specifically adopt an infrared sensor (IR) and / or a thermal conductivity sensor (TC); the gas supply module includes an oxygen tank 10, a carbon dioxide tank 11, a gas mixing structure 12, an electromagnetic valve 13 and a mass flow meter 14, and the oxygen tank 10 and the carbon dioxide tank 11 are connected with the environment cavity in sequence through the gas mixing structure 12, the electromagnetic valve 13 and the mass flow meter 14.

[0047] It should be understood that, for the measurement of the carbon dioxide concentration in the environment cavity, the infrared sensor or the thermal conductivity sensor can be used for detection, or the infrared sensor and the thermal conductivity sensor can be used for cooperative detection.

[0048] In this embodiment, the infrared sensor and the thermal conductivity sensor have robust characteristics, can accurately measure the conditions (carbon dioxide concentration) of the cells, the oxygen tank 10 and the carbon dioxide tank 11 respectively provide pure oxygen and carbon dioxide gas, the oxygen and the carbon dioxide gas can be mixed in a preset ratio through the gas mixing structure 12, the mixed gas enters the mass flow meter 14 for accurate measurement and adjustment, the accurate control of the gas flow rate is ensured, the experimental error caused by unstable gas composition is avoided, and thus according to the accurate measurement result of the carbon dioxide measurement module, the mixed gas with a certain concentration of carbon dioxide is input into the environment cavity through the gas supply module, and specifically the carbon dioxide concentration of the output gas can be ensured to be in the range of 0% to 20%.

[0049] In some embodiments of the present application, the gas supply module is connected to the bottom of the environment cavity, and an air flow plate (not shown) is arranged at the top of the environment cavity and used for forming vertical circulating air flow; the air flow plate is designed to be uniformly distributed with a plurality of holes, and forms a microporous air flow distribution plate.

[0050] In this embodiment, the vertical circulating air flow is formed through the air flow plate at the top of the environment cavity and the air inlet at the bottom, and the carbon dioxide stratification is effectively avoided.

[0051] Further, an axial flow fan (fan) can be arranged in the gas flow direction, and the axial flow fan is selected as a low-noise axial flow fan with a noise of <30 dB, combined with the air flow plate and the air inlet, to stably form a vertical circulating air flow.

[0052] In some embodiments of the present application, as shown in Figure 4 A sterile air filtration system 15 is arranged between the gas supply module and the environment chamber, that is, the mixed gas can enter the environment chamber only after being filtered by the sterile air filtration system.

[0053] In this embodiment, the mixed gas is filtered by the built-in sterile air filtration system 15, which can remove microbial contamination, and is finally sent into the cell culture dish 23, which can provide a sterile and suitable growth environment for cell growth.

[0054] Specifically, the sterile air filtration system in this embodiment can use a h14 grade HEPA filter screen with a microbial filtration efficiency of ≥99.97%.

[0055] In some embodiments of the present application, as shown in Figure 4 The gas mixing structure is a mixing chamber, the gas inlets of the mixing chamber are connected to oxygen gas tanks and carbon dioxide gas tanks respectively, and the gas outlet of the mixing chamber is connected to an electromagnetic valve. Oxygen and carbon dioxide gas enter the mixing chamber in a predetermined ratio for uniform mixing, and then are output to a mass flow meter.

[0056] Further, oxygen and carbon dioxide can be delivered to the mixing chamber by a combination of proportional valves and air pumps to achieve a predetermined ratio of mixed gas.

[0057] In some embodiments of the present application, based on the fact that the carbon dioxide measurement module (IR and TC) will produce a certain drift after being used for a period of time, the cell culture cabin can be provided with an automatic calibration function to automatically calibrate the carbon dioxide measurement module.

[0058] In some embodiments of the present application, the cell culture cabin further comprises a deep ultraviolet LED sterilization module (not shown), which is arranged in the environment chamber and used for sterilizing and disinfecting the culture environment.

[0059] In this embodiment, by integrating a deep ultraviolet (UVC band, 270-280 nm) LED sterilization module in the environment chamber, the environment of the environment chamber can be sterilized and disinfected in advance, effectively killing microorganisms that may affect cell growth, supporting 30-minute high-efficiency disinfection, and killing rate ≥99.9%.

[0060] In some embodiments of the present application, as shown in Figure 1 and Figure 2 The environment cabin comprises an environment cabin lower shell 1 and an environment cabin upper shell 2. The environment cabin upper shell 2 is installed on the top of the environment cabin lower shell 1, and forms an environment chamber with the environment cabin lower shell 1.

[0061] In the embodiment, the environmental cabin is designed in a split type, so that the environmental cavity space can be conveniently opened to take out or put in the culture dish 23.

[0062] Further, the upper shell 2 of the environmental cabin is fixed to the lower shell 1 by a hinged manner and is fixed by a lock.

[0063] Specifically, as shown in the figure, Figure 2 the lock of the present application comprises a lock catch 16 arranged on the lower shell 1 of the environmental cabin and a bayonet 17 arranged on the upper shell 2 of the environmental cabin, and the bayonet 17 is adapted to the lock catch 16.

[0064] In some embodiments of the present application, as shown in the figure, Figure 2 a sealing gasket 18 is arranged between the lower shell 1 of the environmental cabin and the upper shell 2 of the environmental cabin to seal the environmental cavity.

[0065] In the embodiment, by arranging the sealing gasket 18 between the lower shell 1 of the environmental cabin and the upper shell 2 of the environmental cabin to seal the lower shell 1 of the environmental cabin and the upper shell 2 of the environmental cabin, the outside air can be prevented from entering the environmental cavity through the gap between the two, and the culture environment of the environmental cavity can be effectively ensured.

[0066] Further, the sealing gasket 18 can be an annular rubber pad or a silica gel magnetic sealing strip, and the sealing pressure is ≥0.3MPa, and the air tightness test conforms to the ISO 14644-1 clean room standard.

[0067] In some embodiments of the present application, as shown in the figure, Figure 1 and Figure 2 the upper shell 2 of the environmental cabin is provided with a mounting port corresponding to the environmental cavity at the top, and the mounting port is mounted with a transparent glass 3.

[0068] In the embodiment, by mounting the transparent glass 3 at the top of the upper shell 2 of the environmental cabin, the environmental cavity can be observed, so that the cells in the culture dish 23 can be observed by the microscopic equipment without opening the environmental cabin and taking out the culture dish 23.

[0069] In some embodiments of the present application, the transparent glass 3 is an electric heating glass, such as ITO glass.

[0070] In the embodiment, by using the electric heating glass, the dewing phenomenon of the transparent glass 3 can be effectively prevented by controlling the temperature of the transparent glass 3.

[0071] In some embodiments of the present application, the environmental chamber can be formed of modular stainless steel, such as 316L medical-grade stainless steel, which is subjected to electrolytic polishing treatment to achieve a roughness Ra≤0.8 μm, ensuring a sterile operating environment while having excellent corrosion resistance, suitable for humid culture scenarios with a relative humidity > 95%, or the modular stainless steel inner wall is provided with a corrosion-resistant coating to ensure a sterile environment.

[0072] In some embodiments of the present application, the environmental chamber has a size of 20 cm*20 cm*30 cm, capable of accommodating standard culture dishes with a diameter ≤ 15 cm, or other specifications such as ≤ 27.0 cm*57.0 cm*16.5 cm.

[0073] In some embodiments of the present application, as shown in Figure 2 The culture chamber includes a culture chamber lower shell 4 and a culture chamber upper shell 5; the culture chamber lower shell 4 is fixed near the bottom of the environmental chamber; the culture chamber upper shell 5 is installed on the top of the culture chamber lower shell 4, forming a space between the culture chamber lower shell 4 and the culture chamber upper shell 5 for fixing the culture dishes 23, i.e. the culture chamber lower shell 4 and the culture chamber upper shell 5 directly contact the culture dishes 23.

[0074] In this embodiment, by designing the culture chamber in a split type, the culture dishes 23 can be conveniently fixed and removed, avoiding shaking of the culture dishes 23 when moving the environmental chamber.

[0075] Further, the culture chamber lower shell 4 and the culture chamber upper shell 5 are both designed with a hollow middle part, i.e. the culture chamber lower shell 4 and the culture chamber upper shell 5 fix the culture dishes 23 by clamping the edges of the culture dishes 23, to connect the internal space of the culture chamber and the internal space of the environmental chamber, ensuring that the culture dishes 23 are in the environment of the environmental chamber.

[0076] In some embodiments of the present application, as shown in Figure 5 The humidity detection and adjustment system includes a multi-point humidity collection module and a humidity adjustment module 191; the multi-point humidity collection module includes a plurality of humidity sensors 190, respectively arranged at a plurality of points of the environmental chamber, for measuring the humidity of each region of the environmental chamber; the humidity adjustment module 191 is connected with the environmental chamber, for maintaining the environmental chamber at a set humidity.

[0077] In some embodiments of the present application, the humidity sensor is designed based on HDC1080, with an accuracy of ±2%, for collecting humidity information of the environmental chamber, and can be linked with a PT100 sensor or a LMT70 sensor for linkage control.

[0078] In addition, the vertical circulating airflow formed by the air flow plate combined with the air supply module can maintain stable humidity, with a fluctuation degree < ±1% RH.

[0079] In some embodiments of the present application, as shown inFigure 1 and Figure 2 As shown in the figure, the lower shell 1 of the environmental cabin is also provided with a display screen 20, a USB port 21 and a power switch 22, etc., wherein the power switch 22 is used to start the instrument and control the switch of the power supply of the instrument, and the display screen 20 is used to display the state of the instrument.

[0080] In some embodiments of the present application, as shown in the figure, Figure 2 The petri dish 23 can specifically adopt an arrayed microelectrode petri dish, and the arrayed microelectrode petri dish is provided with a probe 24, which can be used to connect an external detection instrument, such as an impedance measuring instrument, to realize signal detection and conduction.

[0081] The present embodiment also proposes a control system, which comprises the cell culture cabin room mentioned above, and further comprises a detection module and a master control module, the detection module can receive detection parameters of the temperature detection and adjustment system, the humidity detection and adjustment system and the carbon dioxide detection and adjustment system and send them to the master control module, and the master control module can control the temperature detection and adjustment system, the humidity detection and adjustment system and the carbon dioxide detection and adjustment system to regulate the temperature, humidity and carbon dioxide concentration of the environmental cavity according to the detection parameters.

[0082] In some embodiments of the present application, as shown in the figure, Figure 6 The master control module can adopt an MCU controller (micro control unit) combined with a clock circuit.

[0083] The MCU controller can specifically select STM32F103RCT6 as the core control part, and the external clock circuit selects an 8M passive crystal oscillator and a 32.768kHz passive crystal oscillator as the system clock and the RTC clock.

[0084] In some embodiments of the present application, as shown in the figure, Figure 6 and Figure 7 The temperature detection and adjustment system can realize accurate temperature regulation based on a PID control algorithm, taking the master control module (MCU controller) as the PID controller, and the PT100 sensor and / or the LMT70 sensor are responsible for real-time detection of the actual temperature of the environmental cavity, converting the temperature signal into an electric signal and transmitting it to the PID controller, the PID controller compares the actual temperature signal of the sensor with the preset target temperature, calculates the deviation, performs PID algorithm operation on the deviation, outputs a control signal, and the semiconductor refrigeration sheet and / or the heating film adjusts the refrigeration / heating power according to the control signal to change the temperature of the environmental cavity.

[0085] Specifically, in the present application, the environmental cavity can be divided into four independent temperature zones, and four groups of temperature sensors 6 are used to monitor the data of the four temperature zones in real time. The temperature (set value) of the environmental cavity (each temperature zone) can be set to 37.0±0.1℃, and the PID algorithm is used for dynamic adjustment and adaptive control. The PID algorithm is as follows:

[0086] Based on the incremental PID algorithm, the heating / cooling power and air flow rate are dynamically adjusted to reduce the overshoot. The output u(t) of the PID controller can be represented by the following formula:

[0087] u(t) = Kp * e(t) + Ki * ∫e(t)dt + Kd * de(t) / dt (1)

[0088] Where:

[0089] u(t) is the output of the PID controller, i.e. the control amount of the actuator.

[0090] e(t) is the error signal, i.e. the difference between the desired value (set value) and the actual output value: e(t) = setpoint(t) - output(t).

[0091] Kp is the proportional gain, which controls the strength of the proportional action.

[0092] Ki is the integral gain, which controls the strength of the integral action.

[0093] Kd is the differential gain, which controls the strength of the differential action.

[0094] ∫e(t)dt is the integral of the error, which represents the cumulative error in the past.

[0095] de(t) / dt is the differential of the error, which represents the rate of change of the error.

[0096] The present application dynamically adjusts the temperature of each temperature zone through the PID algorithm, and uses a multi-point temperature sensor module to obtain temperature data, ensuring high-precision control of environmental parameters, which helps to maintain the optimal conditions for cell growth and improves the reliability and repeatability of experimental results.

[0097] In some embodiments of the present application, as shown in Figure 8 and Figure 9 The main control module detects the concentration of CO2 and automatically controls the mixing of oxygen and carbon dioxide gas at a predetermined ratio, and controls the gas flow rate with a mass flow meter to achieve a CO2 / O2 mixing accuracy of ±0.2%.

[0098] Further, the gas mixing control calculation formula is: error value = detected value - set value, and the amount of delivered gas = time * gas flow rate.

[0099] Specifically, when the CO2 concentration inside the cavity is less than the set value, the mass flow meter is automatically opened for gas delivery, and when the CO2 concentration inside the cavity is greater than the set value, the exhaust is opened to reduce the CO2 concentration.

[0100] In some embodiments of the present application, the control system further comprises a power module to provide stable power support for the entire culture process, ensuring reliable operation of the system.

[0101] In some embodiments of the present application, the power module is composed of a 220V to 12V power adapter and a step-down circuit.

[0102] Among them, the 220V to 12V power adapter selects a power adapter with a maximum output current of 6A to ensure the stability of the system.

[0103] Among them, the step-down circuit includes a 12V to 5V step-down circuit based on DC-DC power module MP2315 and a 5V to 3.3V step-down circuit based on low-voltage linear voltage regulator AMS1117.

[0104] The above is only the preferred specific embodiment of the present application, but the protection scope of the present application is not limited to this. Any person skilled in the art can easily think of changes or replacements within the technical scope disclosed by the present application, which should be covered within the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A cell culture chamber suitable for small-volume equipment, characterized in that, include: An environmental chamber, which forms an environmental cavity; A culture chamber, located within the environmental cavity, is used to fix the culture dish; A temperature detection and regulation system is connected to the ambient cavity to detect and regulate the temperature of the ambient cavity; A humidity detection and regulation system is connected to the environmental cavity to detect and regulate the humidity of the environmental cavity; A carbon dioxide detection and regulation system is connected to the environmental chamber to detect and regulate the carbon dioxide concentration in the environmental chamber.

2. The cell culture chamber according to claim 1, characterized in that, The temperature detection and control system includes: The multi-point temperature sensor module includes multiple sets of temperature sensors, which are respectively located at multiple points in the ambient cavity; The distributed temperature control module includes multiple sets of semiconductor cooling chips and heating films, which are respectively located at multiple positions in the ambient cavity.

3. The cell culture chamber according to claim 1, characterized in that, The carbon dioxide detection and regulation system includes: A carbon dioxide measurement module, including an infrared sensor and / or a thermal conductivity sensor; The gas supply module includes an oxygen cylinder, a carbon dioxide cylinder, a gas mixing structure, a solenoid valve, and a mass flow meter. The oxygen cylinder and the carbon dioxide cylinder are connected to the ambient cavity in sequence via the gas mixing structure, the solenoid valve, and the mass flow meter.

4. The cell culture chamber according to claim 3, characterized in that, The air supply module is connected to the bottom of the environmental chamber, and an airflow plate is provided at the top of the environmental chamber to form a vertical circulating airflow.

5. The cell culture chamber according to claim 3, characterized in that, A sterile air filtration system is provided between the air supply module and the environmental cavity.

6. The cell culture chamber according to claim 1, characterized in that, Also includes: A deep ultraviolet LED sterilization module is located inside the environmental cavity and is used to sterilize and disinfect the culture environment.

7. The cell culture chamber according to claim 1, characterized in that, The environmental chamber includes: Lower shell of the environmental chamber; The upper shell of the environmental chamber is installed on top of the lower shell of the environmental chamber, forming the environmental cavity between the upper shell and the lower shell of the environmental chamber; A sealing gasket is disposed between the lower shell and the upper shell of the environmental chamber to seal the environmental cavity.

8. The cell culture chamber according to claim 7, characterized in that, The environmental chamber also includes: The environmental chamber has a transparent glass panel, and an installation port for installing the transparent glass panel is provided on the top of the upper shell corresponding to the environmental cavity. The transparent glass is electrically heated glass.

9. The cell culture chamber according to claim 1, characterized in that, The culture chamber includes: The lower shell of the culture chamber; The upper shell of the culture chamber is installed on top of the lower shell of the culture chamber, forming a space between the upper shell and the lower shell of the culture chamber for fixing the culture dish.

10. A control system, characterized in that, The control system includes the cell culture chamber as described in any one of claims 1 to 9, and further includes a detection module and a main control module. The detection module is capable of receiving detection parameters from the temperature detection and regulation system, the humidity detection and regulation system, and the carbon dioxide detection and regulation system and sending them to the main control module. The main control module is capable of controlling the temperature detection and regulation system, the humidity detection and regulation system, and the carbon dioxide detection and regulation system to regulate the temperature, humidity, and carbon dioxide concentration of the environmental chamber according to the various detection parameters.

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