Sleep-aiding eurotium cristatum Fuzhuan tea and preparation method thereof

By using targeted sustained-release functional additives and a two-stage variable-temperature fermentation process, the problems of stability and targeted delivery of traditional Chinese medicine components in sleep-aiding Fu brick tea have been solved, achieving efficient enrichment of GABA and sedative neuropeptides, ensuring the product's clear efficacy and pure flavor.

CN121196038AInactive Publication Date: 2025-12-26SHAANXI SHENGFENGDA IND & TRADE CO LTD
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Patent Information

Application Number
CN202511689634.7
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-11-18
Publication Date
2025-12-26
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

Existing technologies for developing sleep-aiding Fu brick tea cannot guarantee the synergistic effect between the effective components of traditional Chinese medicine and the tea system. Additives are easily degraded, resulting in unstable efficacy. Furthermore, there is a lack of consideration for targeted delivery and efficient absorption, making it difficult to meet the needs of modern consumers for clearly defined functions and mechanisms.

Method used

By employing targeted sustained-release functional adjuvants and a two-stage variable-temperature fermentation process, a microencapsulated functional component delivery system is constructed through a pH-responsive chitosan-pectin complex. This system precisely regulates the life cycle of *Aspergillus cristatus*, enabling the targeted enrichment and efficient utilization of GABA and sedative neuropeptides.

Benefits of technology

This ensures high bioavailability of functional ingredients, consistent product efficacy, and pure flavor, achieving a natural, safe, and clearly effective sleep aid.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention discloses sleep-aiding eurotium cristatum Fuzhuan tea and a preparation method thereof. The efficacy and scientificity of a product are improved. In a tea leaf fermentation stage, by partially releasing L-glutamic acid, casein and prebiotics, a substrate is provided for an enzyme system secreted by eurotium cristatum, and gamma-aminobutyric acid and sedative neuropeptide are directionally converted and enriched; in the drinking stage of a human body, the targeted slow-release functional auxiliary agent takes a pH response type chitosan-pectin compound as a coating to protect unutilized precursors to be released at fixed points in intestinal tracts, in-situ biotransformation is carried out, the bioavailability is improved, and the sleep aiding effect is improved. According to the two-stage variable-temperature fermentation process, field planting of eurotium cristatum is promoted through low temperature and high humidity in the early stage, key metabolic enzyme activity is activated through temperature rise and humidity reduction in the later stage, and precursor conversion is efficiently catalyzed. The invention provides the preparation method of the sleep-aiding eurotium cristatum Fuzhuan tea which is natural, safe, remarkable in effect and excellent in flavor.
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Description

TECHNICAL FIELD

[0001] The present application relates to the field of functional tea processing technology, in particular to a sleep-aiding golden-flower brick tea and a preparation method thereof. BACKGROUND

[0002] The brick tea belongs to the black tea category and is a unique post-fermented tea in China, which is famous for its unique "flowering" process. Under specific temperature and humidity conditions, Eurotium cristatum will naturally grow in the brick tea, and its closed asci will appear golden spots under a microscope, which is commonly known as "golden flower". The "golden flower" not only constitutes the unique "fungus flower aroma" and mellow taste of the brick tea, but also helps to degrade starch, protein, polyphenols and other substances in the tea through the metabolism of various active enzymes, thereby improving the quality and flavor of the tea. Studies have shown that Eurotium cristatum has excellent probiotic properties, and the brick tea is believed to have many health benefits such as digestion, weight loss, and intestinal flora regulation.

[0003] With the growing demand for functional tea products in the market, developing brick tea with sleep-aiding and tranquilizing effects has become a new research direction. The existing technology mainly adds extracts or powders of tranquilizing traditional Chinese medicines such as jujube kernel, lily, and polygala to the brick tea, or adjusts the fermentation process parameters to improve the health value. However, these methods have obvious limitations: first, simple physical mixing cannot ensure the synergistic effect of the effective components of traditional Chinese medicines and the tea system and fermentation microorganisms, and the added substances may affect the inherent flavor and quality of the brick tea; second, the exogenous sleep-aiding components are prone to degradation or transformation during fermentation, drying and subsequent storage, resulting in unstable product efficacy and poor reproducibility; more importantly, such methods are mostly based on empirical compounding, and lack consideration of the targeted delivery and efficient absorption of active ingredients in the human body, with unclear action mechanism, which cannot meet the higher requirements of modern consumers for functional foods with clear efficacy and mechanism. Therefore, developing a sleep-aiding golden-flower brick tea with deep integration with the fermentation system of the brick tea, clear efficacy components and high bioavailability, and a preparation method thereof, has become a technical problem to be solved in the field.

[0004] In view of this, the present application provides a sleep-aiding golden-flower brick tea and a preparation method thereof, which has great significance. SUMMARY

[0005] The present application aims to provide a sleep-aiding golden-flower brick tea and a preparation method thereof to solve the problems in the background art.

[0006] To achieve the above-mentioned purpose, the present application provides the following technical solutions: A preparation method of a sleep-aiding golden-flower brick tea, comprising the following preparation steps: Step one, selecting and sieving: select eucommia black tea as raw material, remove impurities, then use a pulverizer to cut the black tea after impurities removal into regular segments and sieve, to obtain tea leaves.

[0007] Step two, pile: the tea is treated by pile for 24-48h, and is turned over for 1-2 times, to obtain the tea after pile.

[0008] Step three, steaming: the tea after pile is placed in a steaming equipment for 8-12min, the temperature of the saturated steam is 100-105℃, after steaming, the tea is spread to cool, to obtain the tea after steaming.

[0009] Step four, adding functional additives: the tea after steaming is placed in a rotary blender, 2%-4% of the weight of the tea of the targeted slow-release functional additive is added, and 20%-30% of the weight of the tea of pure water is sprayed, stirring for 10-20min, to obtain uniformly mixed tea.

[0010] Step five, fermentation: the uniformly mixed tea is placed in a mold to press into a brick type, and is placed in a fermentation room, a two-stage variable temperature fermentation method is adopted, the fermentation temperature is 25-30℃ and the humidity is 75%-85% for the first 7 days, the fermentation temperature is 30-35℃ and the humidity is 65%-75% for the 8th to 15th day, to obtain the fermented tea.

[0011] Step six, drying: the fermented tea is first dried to a water content of 10%, and then is placed in an aging warehouse for 20-35 days, to obtain the sleep-aiding golden flower fuzhou brick tea.

[0012] As a preferred technical solution of the present application, the targeted slow-release functional additive is prepared by the following steps: S1, core material preparation: 10-30 parts by weight of L-glutamic acid, 5-15 parts by weight of casein, 5-15 parts by weight of fructooligosaccharide, 10-20 parts by weight of trehalose, and 30-50 parts by weight of porous starch are placed in a mixer and mixed at a speed of 30-50 rpm for 15-30 min to obtain a core material mixture S2, coating liquid preparation: a chitosan acetic acid solution and a pectin aqueous solution are mixed at a volume ratio of 1:1, and a magnetic stirrer is used to stir at a speed of 500-800 rpm for 30-40 min to obtain a coating liquid.

[0013] S3, fluidized bed coating: the core material mixture is added to the hopper of the fluidized bed granulator, and the coating liquid is used for coating until the coating weight gain reaches 15%-30% of the weight of the core material mixture, the coating is finished, the material is discharged, and the targeted slow-release functional additive is obtained.

[0014] As a preferred technical solution of the present application, the speed of the rotary blender in step four is 15-25 rpm.

[0015] As a preferred technical solution of the present application, the temperature of the drying in step six is 60-70 DEG C.

[0016] As a preferred technical solution of the present application, the mass fraction of the chitosan acetic acid solution and the pectin water solution is 1-3%.

[0017] As a preferred technical solution of the present application, the inlet air temperature of the fluidized bed granulator is 35-45 DEG C, the atomization pressure is 0.2-0.4 MPa, and the liquid spraying rate is 2-5 mL / min.

[0018] Compared with the prior art, the present application has the following beneficial effects: 1. The present application introduces a targeted slow-release functional aid to construct a precise functional component delivery system, improving the certainty and scientificity of the sleep-aiding Jinhua Fuzhuan tea efficacy. The aid uses a pH-responsive chitosan-pectin compound as a wall material, which can effectively resist the erosion of gastric acid environment on the core material, ensuring that the core functional precursors and prebiotics can reach the intestine intact and achieve site-specific release. Not only does it improve the bioavailability of the precursor substances, but also enables them to be efficiently utilized by the microbial flora, thereby efficiently enriching GABA and sedative neuropeptides.

[0019] 2. The present application adopts a two-stage variable-temperature fermentation process to precisely control the life cycle of Eurotium cristatum, guiding the fermentation process from simple microbial growth to efficient synthesis of target functional components. The process promotes the full colonization and biomass accumulation of mycelium in the early stage, and then increases the temperature and reduces the humidity in the later stage, significantly stimulating the enzyme activity of glutamate decarboxylase (GAD), thereby efficiently catalyzing the conversion of precursor substances released by the functional aid into GABA and sedative neuropeptides.

[0020] 3. The present application perfectly coordinates the two-stage variable-temperature fermentation process with the release kinetics of the targeted slow-release functional aid, forming an efficient mode of "pre-bacteria and post-material, synergistic conversion". Not only does it achieve the directional maximum enrichment of functional components, but also effectively avoids the accumulation of metabolic by-products and flavor defects caused by single constant-temperature fermentation, ensuring the unity of excellent functionality and pure flavor of the product, and providing a natural and safe sleep-aiding tea solution with clear efficacy. DETAILED DESCRIPTION

[0021] The technical solutions in the embodiments of the present application will be described below in a clear and complete manner. Obviously, the described embodiments are only a part of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0022] The targeted slow-release functional aid is a microencapsulated functional component delivery system constructed based on a pH-responsive polymer, and realizes directional enrichment and efficient utilization of the sleep-aiding component GABA and the sedative neuropeptide in the tea processing stage and the human drinking stage, respectively. In the fermentation process of the brick tea, the coating of the aid releases part of the functional precursors (L-glutamic acid, casein) and prebiotics (fructooligosaccharides) in the humid microenvironment inside the tea leaves in advance, and the L-glutamic acid and casein can be utilized by the glutamate decarboxylase and protease system in the tea during the fermentation of Eurotium cristatum, and are converted into GABA and sedative neuropeptides inside the tea. The prebiotics can selectively promote the growth and metabolic activity of Eurotium cristatum. After drinking, a large amount of precursors still not released and protected by the aid are accurately delivered to the intestinal tract, realize controllable release, and can be directly utilized by the intestinal microflora for in-situ bioconversion, and play a systematic sleep-aiding regulation function through the gut-brain axis pathway.

[0023] The targeted slow-release functional aid can overcome the damage of gastric acid to active precursor substances, maximize the delivery efficiency, realize the targeted delivery of functional components, efficiently enrich GABA and sedative neuropeptides, and solve the problem of low bioavailability of oral functional components. Through the microcapsule technology, the precursors are standardized and stabilized, unnecessary chemical reactions or losses in the processing and storage process are avoided, and the uniformity and reliability of the efficacy of each batch of products are ensured. After release, the components such as fructooligosaccharides in the targeted slow-release functional aid can act as prebiotics to selectively promote the growth of intestinal beneficial bacteria and Eurotium cristatum itself, and further strengthen the intestinal health and sleep-aiding dual efficacy of the product.

[0024] Glutamic acid, as a direct biosynthetic precursor of GABA, is directly decarboxylated to GABA under the catalysis of GAD contained in Eurotium cristatum, and its high addition amount ensures that GABA can be effectively enriched in the final product, which is the core material basis for realizing the sleep-aiding function.

[0025] Casein, as a high-quality protein, is hydrolyzed into various small molecular peptides and amino acids under the action of protease and peptidase secreted by Eurotium cristatum, and these hydrolysis products contain sedative neuropeptides (such as beta-casomorphin) with opioid activity, which can indirectly play a central sedative role by interacting with opioid receptors in the intestinal tract, and is another important source of sleep-aiding functional components.

[0026] Fructooligosaccharides, as a soluble dietary fiber, selectively promote the growth and metabolic activity of Eurotium cristatum. It cannot be utilized by most miscellaneous bacteria, but can be efficiently utilized by Eurotium cristatum, thereby consolidating its species dominance in the fermentation system, ensuring the vigorous and pure “flowering” process, and helping to maintain the health of the intestinal flora.

[0027] Trehalose has excellent stress resistance, which can protect L-glutamic acid, casein and other active ingredients and Eurotium cristatum itself from inactivation due to high temperature, dehydration and other stresses during processing, drying and storage. At the same time, trehalose itself has moderate sweetness, which can gently improve the taste of tea soup and mask potential bad flavors.

[0028] Porous starch has a huge specific surface area and pore volume, and its core function is to physically adsorb and fix small molecular substances such as L-glutamic acid and trehalose to form a solid-state mixed core material with uniform composition. This ensures that the components can be uniformly dispersed during the subsequent fluidized bed coating process and prevents caking during storage, which is the basic foundation for building a stable core material.

[0029] Food-grade sepiolite, as a fibrous silicate mineral, cooperates with porous starch to form a more stable core material structure. Through its ion exchange and adsorption properties, it adjusts the final release rate of the core material content in the intestinal tract to avoid rapid release. Its good particle characteristics help improve the flowability of the mixed powder, making it easier to operate in subsequent coating processes, and also prevent the core material mixture from absorbing moisture and caking.

[0030] The two-stage variable-temperature fermentation method adopted in the present application is a precise fermentation strategy based on stage process regulation of Eurotium cristatum growth and metabolism dynamics. The strategy optimizes the colonization and metabolic synthesis of microorganisms by setting different temperature and humidity parameters in different fermentation stages, thereby realizing the efficient enrichment of functional components in cooperation with the targeted slow-release functional adjuvant. The first stage is the mycelium colonization and biofilm formation period (low temperature and high humidity). The mild temperature and high humidity environment provides the most suitable physiological conditions for spore germination and mycelial growth of Eurotium cristatum, enabling the fungus to colonize uniformly and luxuriantly inside the tea brick and form a strong biomass basis. At the same time, the higher humidity effectively prevents the premature loss of water on the surface of the tea brick, ensuring the water activity required for fermentation. In this stage, the prebiotics released from the functional adjuvant, such as fructooligosaccharides, provide a specific carbon source for the rapid proliferation of Eurotium cristatum, enabling it to dominate in microbial competition. The second stage is the directional transformation and secondary metabolism acceleration period (temperature rise and humidity reduction). The temperature rise is a key metabolic switch signal. The elevated temperature can significantly increase the specific activity and expression of glutamate decarboxylase (GAD) and various proteases in Eurotium cristatum. GAD is a key rate-limiting enzyme that catalyzes the decarboxylation of L-glutamic acid to GABA. At this time, the targeted slow-release functional adjuvant has released high concentrations of L-glutamic acid and casein inside the tea brick. In the optimized enzyme activity environment, the fungus can efficiently utilize these precursor substances to directionally transform them into the target product-GABA and a series of biologically active peptides with sedative function. The slight reduction in humidity creates a slight stress environment, which, in cooperation with the temperature rise, further promotes the microorganisms to shift resources from growth to synthesis of secondary metabolites with stress protection function (such as GABA). The temperature and humidity conditions in this stage are also conducive to the formation and retention of volatile flavor substances, avoiding the generation of "fermentation stench" that affects quality, thereby shaping the unique flavor of the product with a sweet and mellow taste.

[0031] The present application provides a kind of sleep-aiding gold flower fuzhuan tea and its production method technical scheme: Example one

[0032] A sleep-aiding gold flower fuzhuan tea production method is characterized by comprising the following preparation steps: Step one, material selection and sieving: select Eucommia black tea as raw material, remove impurities, then use a pulverizer to cut the black tea after impurity removal into regular segments and sieve, to obtain tea leaves.

[0033] Step two, pile up: pile up the tea leaves for 36h, and turn over once to obtain the tea leaves after piling up.

[0034] Step three, steaming: steam the tea leaves after piling up in a steam equipment for 10min, the temperature of saturated steam is 103℃, after steaming, spread the tea leaves to cool down, to obtain the tea leaves after steaming.

[0035] Step four, add functional additives: Put the steamed tea leaves into a rotary blender, add 3% of the weight of the tea leaves of targeted slow-release functional additives, and spray 25% of the weight of the tea leaves of pure water. Stir for 15 minutes at a speed of 20 rpm to obtain uniformly mixed tea.

[0036] Step five, fermentation: Put the uniformly mixed tea into a mold to press into a brick, and place it in a fermentation room. Use a two-stage variable temperature fermentation process. The fermentation temperature is 27°C and the humidity is 80% for the first 7 days, and the fermentation temperature is 33°C and the humidity is 70% for the 8th to 15th day. Obtain fermented tea leaves.

[0037] Step six, drying: First, dry the fermented tea leaves at 65°C until the moisture content is 10%, then place them in an aging warehouse for 27 days to obtain the sleep-aiding golden flower brick tea.

[0038] The targeted slow-release functional additive is prepared by the following steps: S1, core material preparation: Put 20 parts by weight of L-glutamic acid, 10 parts by weight of casein, 10 parts by weight of fructooligosaccharide, 15 parts by weight of trehalose, and 40 parts by weight of porous starch into a mixer. Mix at a speed of 40 rpm for 22 minutes to obtain a core material mixture.

[0039] S2, coating liquid preparation: Mix a 2% by mass chitosan acetic acid solution and a 2% by mass pectin water solution in a volume ratio of 1:1, and use a magnetic stirrer to stir at a speed of 650 rpm for 35 minutes to obtain a coating liquid.

[0040] S3, fluidized bed coating: Add the core material mixture to the hopper of the fluidized bed granulator, and use the coating liquid for coating. The inlet air temperature of the fluidized bed granulator is 40°C, the atomization pressure is 0.3 MPa, and the liquid spray rate is 3 mL / min. When the coating weight reaches 22% of the weight of the core material mixture, the coating is complete. Discharge to obtain the targeted slow-release functional additive.

[0041] Example two

[0042] A sleep-aiding golden flower brick tea production method, characterized by comprising the following preparation steps: Step one, material selection and sieving: Select Eucommia black tea as the raw material, remove impurities, then use a pulverizer to cut and sieve the impurities-removed black tea into regular pieces to obtain tea leaves.

[0043] Step two, pile up: Pile up the tea leaves for 24 hours, and perform secondary pile turning to obtain piled-up tea leaves.

[0044] Step three, steaming: Steam the piled-up tea leaves in a steam equipment for 8 minutes. The temperature of the saturated steam is 100°C. After steaming, spread the tea leaves to cool down to obtain steamed tea leaves.

[0045] Step four, add functional aids: After steaming, the tea leaves are placed in a rotary blender, 2% of the tea leaf weight of targeted slow-release functional aids are added, and 20% of the tea leaf weight of pure water is sprayed. Stir at a speed of 15 rpm for 10 min to obtain uniformly mixed tea.

[0046] Step five, fermentation: The uniformly mixed tea is placed in a mold to press into a brick, and is placed in a fermentation room. A two-stage variable temperature fermentation process is used, with a fermentation temperature of 25°C and a humidity of 75% for the first 7 days, and a fermentation temperature of 30°C and a humidity of 65% for the 8th to 15th days. The fermented tea is obtained.

[0047] Step six, drying: The fermented tea is first dried at 60°C until the moisture content is 10%, and then is placed in an aging warehouse for 20 days to obtain the sleep-aiding golden flower brick tea.

[0048] The targeted slow-release functional aid is prepared by the following steps: S1, core material preparation: 10 parts by weight of L-glutamic acid, 5 parts by weight of casein, 5 parts by weight of fructooligosaccharide, 10 parts by weight of trehalose, and 30 parts by weight of porous starch are placed in a mixer and mixed at a speed of 40 rpm for 15 min to obtain a core material mixture.

[0049] S2, coating liquid preparation: A 1% by mass chitosan acetic acid solution and a 1% by mass pectin aqueous solution are mixed at a volume ratio of 1:1 and stirred at a speed of 500 rpm for 30 min using a magnetic stirrer to obtain a coating liquid.

[0050] S3, fluidized bed coating: The core material mixture is added to the hopper of a fluidized bed granulator, and the coating liquid is used for coating. The inlet air temperature of the fluidized bed granulator is 35°C, the atomization pressure is 0.2 MPa, and the liquid spraying rate is 2 mL / min. When the coating weight gain reaches 15% of the weight of the core material mixture, the coating is completed, the material is discharged, and the targeted slow-release functional aid is obtained.

[0051] Example three

[0052] A sleep-aiding golden flower brick tea production method, characterized by comprising the following preparation steps: Step one, material selection and sieving: Selecting Eucommia black tea as the raw material, removing impurities, and then using a pulverizer to cut and sieve the impurities-removed black tea into regular pieces to obtain tea leaves.

[0053] Step two, pile fermentation: The tea leaves are subjected to pile fermentation for 48 h, with 2 times of pile turning, to obtain pile-fermented tea leaves.

[0054] Step three, steaming: the tea after fermentation is placed in a steaming device for steaming for 12 min, the temperature of the saturated steam is 105℃, after steaming, the tea is spread out to cool, and the tea after steaming is obtained.

[0055] Step four, adding functional additives: the tea after steaming is placed in a rotary blender, 4% of the weight of the tea of the targeted slow-release functional additive is added, and 30% of the weight of the tea of pure water is sprayed, stirring at a speed of 25 rpm for 20 min to obtain uniformly mixed tea.

[0056] Step five, fermentation: the uniformly mixed tea is placed in a mold to press into a brick type, and is placed in a fermentation room, adopting a two-stage variable temperature fermentation process, the fermentation temperature is 30℃ and the humidity is 85% for the first 7 days, and the fermentation temperature is 35℃ and the humidity is 75% for the 8th to 15th day, to obtain the fermented tea.

[0057] Step six, drying: the fermented tea is first dried at 70℃ until the moisture content is 10%, and then is placed in an aging warehouse for aging for 35 days to obtain the sleep-aiding golden flower brick tea.

[0058] The targeted slow-release functional additive is prepared by the following steps: S1, core material preparation: 30 parts by weight of L-glutamic acid, 15 parts by weight of casein, 15 parts by weight of fructooligosaccharide, 20 parts by weight of trehalose, and 50 parts by weight of porous starch are placed in a mixer and mixed at a speed of 50 rpm for 30 min to obtain a core material mixture.

[0059] S2, coating liquid preparation: a chitosan acetic acid solution with a mass fraction of 3% and a pectin aqueous solution with a mass fraction of 3% are mixed at a volume ratio of 1:1, and a magnetic stirrer is used to stir at a speed of 800 rpm for 40 min to obtain a coating liquid.

[0060] S3, fluidized bed coating: the core material mixture is added to the hopper of the fluidized bed granulator, and the coating liquid is used for coating, the inlet air temperature of the fluidized bed granulator is 45℃, the atomization pressure is 0.4MPa, and the liquid spraying rate is 5mL / min, until the coating weight gain reaches 30% of the weight of the core material mixture, the coating is completed, the material is discharged, and the targeted slow-release functional additive is obtained.

[0061] Comparative Example One: The difference from Example One is that no targeted slow-release functional additive is introduced.

[0062] Comparative Example Two: The difference from Example One is that the two-stage variable temperature fermentation process is not used, i.e., the fermentation process is carried out at a temperature of 25-30℃ and a humidity of 75%-85%.

[0063] Test Method: 1. In vitro opiate receptor agonism rate determination: tea samples are extracted with phosphate buffer solution at pH = 7.4 to obtain the test solution. In the experimental wells containing a fixed amount of opiate receptors (μ type), the labeled competitive ligand naloxone and the above tea sample test solution are added at the same time. If there is an opiate-like active substance in the tea sample, it will compete with the labeled ligand for binding to the receptor. Then, the unbound substances are washed away, and the amount of labeled substance bound to the receptor is detected. By calculating the inhibition rate of the tea sample on the binding of the standard ligand, the opiate receptor agonism activity is characterized, and the higher the inhibition rate, the stronger the activity.

[0064] 2. Mouse sleep prolongation rate determination: mice are divided into 5 experimental groups (gavaged with the tea soup of the present application) and one control group (gavaged with the same amount of normal saline or the tea soup of the comparative example), with 5 mice in each group. After a period of gavage, a subthreshold dose of sodium pentobarbital is injected intraperitoneally into all mice. Immediately observe the mice, take the disappearance of the righting reflex as the sign of falling asleep, and record the number of sleeping animals and the duration of sleep. Compare the average sleep time of the experimental group with that of the control group, calculate the sleep prolongation rate, and evaluate the sleep-aiding effect of the product.

[0065] The GABA content is detected according to the national standard GB / T 34789-2017 "Determination of γ-aminobutyric acid in tea - High performance liquid chromatography"; and the tea sensory evaluation is carried out by a professional evaluation group according to the national standard GB / T 23776-2018.

[0066] Table 1

[0067] As shown in the results in Table 1, compared with Examples 1, 2 and 3, the GABA content, in vitro opiate receptor agonism rate and mouse sleep prolongation rate of Comparative Example 1 are all significantly reduced, proving that the "targeted slow-release functional aid" used in the present application has the functions of enriching GABA, generating sedative neuropeptides with opiate-like activity, and ultimately achieving a significant sleep-aiding effect of the product, and can also improve the flavor quality of the sleep-aiding Jinfu brick tea. Compared with Examples 1, 2 and 3, the GABA content, in vitro opiate receptor agonism rate and mouse sleep prolongation rate of Comparative Example 2 are all significantly lower than those of Example 1, proving that the "two-stage variable temperature fermentation method" used in the present application has a significant synergistic effect on enriching GABA, promoting the generation of sedative neuropeptides and improving the sleep-aiding effect of the final product, and its effect is better than that of the conventional constant temperature fermentation process.

[0068] The above is only a specific embodiment of the present application, but the technical features of the present application are not limited thereto. Any simple change, equivalent replacement or modification made on the basis of the present application to solve basically the same technical problem and achieve basically the same technical effect is also covered by the protection scope of the present application.

Claims

1. A method for preparing a sleep-aiding Jinfu brick tea, characterized in that, The preparation process comprises the following steps: Step one, selecting and sieving: select eucommia black tea as raw material, remove impurities, then use a pulverizer to cut the black tea into regular pieces and sieve, to obtain tea leaves; Step two, pile up: pile up the tea leaves for 24-48h, turn over 1-2 times, to obtain tea leaves after piling up; Step three, steaming: steam the tea leaves in a steam equipment for 8-12min, the temperature of the saturated steam is 100-105℃, after steaming, spread the tea leaves to cool, to obtain tea leaves after steaming; Step four, add functional additives: put the tea leaves after steaming into a rotary blender, add 2%-4% of the weight of the tea leaves of the targeted slow-release functional additives, and spray 20%-30% of the weight of the tea leaves of pure water, stir for 10-20min, to obtain uniformly mixed tea; Step five, fermentation: put the uniformly mixed tea into a mold to press into a brick, put it into a fermentation room, adopt two-stage variable temperature fermentation process, the fermentation temperature is 25-30℃ and the humidity is 75%-85% in the first 7 days, the fermentation temperature is 30-35℃ and the humidity is 65%-75% in the 8th-15th day, to obtain fermented tea; Step six, drying: first dry the fermented tea to a moisture content of 10%, then put it into an aging warehouse for 20-35 days, to obtain the sleep-aiding gold-flower-fermented brick tea.

2. The method for preparing the sleep-aiding Jinfu brick tea according to claim 1, characterized in that, The targeted slow-release functional additive is prepared by the following steps: S1, core material preparation: put 10-30 parts by weight of L-glutamic acid, 5-15 parts by weight of casein, 5-15 parts by weight of fructooligosaccharide, 10-20 parts by weight of trehalose, and 30-50 parts by weight of porous starch into a mixer, mix at a speed of 30-50 rpm for 15-30 min, to obtain a core material mixture; S2, coating liquid preparation: mix chitosan acetic acid solution and pectin aqueous solution at a volume ratio of 1:1, and use a magnetic stirrer to stir at a speed of 500-800 rpm for 30-40 min, to obtain a coating liquid; S3, fluidized bed coating: put the core material mixture into the hopper of the fluidized bed granulator, coat with the coating liquid, until the coating weight gain reaches 15%-30% of the weight of the core material mixture, then stop coating, discharge, to obtain the targeted slow-release functional additive.

3. The method for preparing the sleep-aiding Jinfu brick tea according to claim 1, characterized in that, The speed of the rotary blender in step four is 15-25 rpm.

4. The method for preparing the sleep-aiding Jinfu brick tea according to claim 1, characterized in that, The temperature of the drying in step six is 60-70℃.

5. The method for preparing the sleep-aiding Jinfu brick tea according to claim 2, characterized in that, The mass fraction of the chitosan acetic acid solution and the pectin aqueous solution is 1-3%.

6. The method for preparing the sleep-aiding Jinfu brick tea according to claim 2, characterized in that, The inlet air temperature of the fluidized bed granulator is 35-45℃, the atomization pressure is 0.2-0.4 MPa, and the liquid spraying rate is 2-5 mL / min.

7. A sleep-aiding Jinfu brick tea, characterized in that, Prepared by the preparation process of any one of claims 1-6.