Composition with tightening, moisturizing and repairing effects as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of cosmetics, and discloses a composition with tightening, moisturizing and repairing effects as well as a preparation method and application thereof. The composition is prepared from the following components in parts by mass: 1 to 30 parts of elastin, 1 to 30 parts of fibronectin, 1 to 40 parts of milk protein and 1 to 35 parts of agilawood extract. The composition disclosed by the invention is mild and non-irritant, is good in formula stability, and has multiple skin care effects of tightening, moisturizing and repairing, so that the composition has a wide application prospect in the development of cosmetics.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, and specifically relates to compositions with firming, moisturizing and repairing effects, their preparation methods and applications. Background Technology

[0002] With rising living standards and evolving aesthetic standards, cosmetics have become an indispensable part of daily skincare. Consumers' demands for cosmetic efficacy have evolved from basic moisturizing and cleansing to the pursuit of comprehensive products with multiple functions. However, many cosmetics on the market still suffer from limited efficacy and fail to simultaneously meet the diverse needs of consumers.

[0003] Currently, cosmetic formulation technologies focused on single-function ingredients are relatively mature. For example, formulations based on ingredients such as hyaluronic acid, glycerin, and panthenol can achieve good moisturizing effects; formulations based on ingredients such as retinol and peptides are mainly aimed at skin firming and anti-wrinkle. Although the above-mentioned single-function products have achieved certain results in their respective fields, consumers need to use multiple products in sequence in their daily skin care routine, which is cumbersome and increases time and economic costs.

[0004] To address this issue, the industry has made some attempts, such as simple physical compounding of multiple active ingredients. However, this mixing method often has many limitations: different active ingredients may be incompatible, antagonistic to each other and reduce efficacy; blindly adding multiple ingredients in order to achieve multiple effects may lead to overly complex formulas, unstable formula systems, and increased risk of skin irritation.

[0005] Therefore, developing a product with a stable formula system, multiple skin care benefits, and safety and gentleness has become a technical problem that urgently needs to be solved in this field. Summary of the Invention

[0006] The present invention aims to solve at least one of the technical problems existing in the prior art. To this end, the present invention provides a composition with firming, moisturizing and repairing effects, a method for preparing the composition and its application. The composition is stable, mild and non-irritating, and has multiple skin care effects of firming, moisturizing and repairing.

[0007] In a first aspect, the present invention provides a composition comprising, by weight parts: 1-30 parts elastin, 1-30 parts fibronectin, 1-40 parts milk protein and 1-35 parts agarwood extract.

[0008] Specifically, the mass fraction of the elastin can be any point value or any two-point range value between 1 and 30 parts, such as 1 part, 5 parts, 10 parts, 15 parts, 20 parts, 25 parts, 30 parts, etc.

[0009] Specifically, the mass fraction of the fibronectin can be any point value or any two-point range between 1 and 30 parts, such as 1 part, 5 parts, 10 parts, 15 parts, 20 parts, 25 parts, 30 parts, etc.

[0010] Specifically, the mass fraction of the milk protein can be any point value or any two points between 1 and 40 parts, such as 1 part, 5 parts, 10 parts, 15 parts, 20 parts, 25 parts, 30 parts, 35 parts, 40 parts, etc.

[0011] Specifically, the mass fraction of the agarwood extract can be any point value or any two points between 1 and 35 parts, such as 1 part, 5 parts, 10 parts, 15 parts, 20 parts, 25 parts, 30 parts, 35 parts, etc.

[0012] In some embodiments of the present invention, the composition comprises, by weight parts, the following components: 1-25 parts of elastin, 1-25 parts of fibronectin, 1-35 parts of milk protein, and 1-30 parts of agarwood extract.

[0013] In some embodiments of the present invention, the composition comprises, by weight parts, the following components: 8-14 parts of elastin, 7-13 parts of fibronectin, 12-18 parts of milk protein, and 8-14 parts of agarwood extract.

[0014] Specifically, the elastin described in this invention comprises glycine, proline, and other amino acids, which can form β-turns and a relatively wide β-helix. The interior of the helix contains a large number of hydrophobic amino acid side chains, giving the elastin good hydrophilicity and lipophilicity, helping the skin to better absorb nutrients. Elastin can also help form more collagen, slowing down physiological skin aging caused by the external environment. It also has antioxidant capacity, resisting damage from the external environment and maintaining the healthy state of the skin. In addition, elastin is a molecular determinant of late-stage arterial morphogenesis, and can regulate the proliferation and tissue formation of vascular smooth muscle, thereby stabilizing arterial structure and achieving anti-wrinkle effects.

[0015] Specifically, the fibronectin described in this invention, as an important component of the skin's extracellular matrix, can promote the secretion of glycoprotein molecules such as hyaluronic acid, providing excellent water retention and moisturizing effects. At the same time, as a key signaling protein, fibronectin can activate collagen in the extracellular matrix, stimulate the production of hyaluronic acid and other proteins, change the normal arrangement of the extracellular matrix, and play an anti-wrinkle role. In addition, it can also inhibit and block the excessive production of melanin, improve the skin's microcirculation, and thus maintain the skin's healthy state.

[0016] Specifically, the milk protein described in this invention can enhance the moisturizing effect of the outer layer of skin, reduce redness and swelling caused by skin allergies, thereby accelerating skin repair. It can also reduce skin pigmentation caused by melanin produced by melanocytes both in vivo and in vitro. Furthermore, the amino acids in the protein provide hydrogen bonds and hydrophobic interactions, rapidly forming a "physical firming" effect, similar to bovine serum albumin, which is known for its instant firming effect, resulting in younger and firmer skin.

[0017] Specifically, the agarwood extract described in this invention has certain antioxidant, anti-aging, and anti-inflammatory effects, and it also inhibits hyaluronidase, effectively inhibiting the decomposition of hyaluronic acid, thus having a good moisturizing effect.

[0018] In some embodiments of the present invention, the composition further includes a solvent.

[0019] In some embodiments of the present invention, the composition further includes 1-70 parts by weight of solvent.

[0020] In some embodiments of the present invention, the solvent includes at least one selected from butanediol, 1,2-hexanediol, 1,3-propanediol, propylene glycol, and 1,2-pentanediol.

[0021] A second aspect of the present invention provides a method for preparing the composition described in the first aspect of the present invention, comprising the following steps: The components are mixed to obtain the composition.

[0022] A third aspect of the present invention provides a cosmetic product comprising the composition described in the first aspect of the present invention.

[0023] In some embodiments of the present invention, the composition is present in a cosmetic at a mass content of 0.1%-10%.

[0024] Specifically, the mass content of the composition in the cosmetic can be any point value or any two-point range value between 0.1% and 10%, such as 0.1%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, etc.

[0025] In some embodiments of the present invention, the cosmetic further includes at least one of a thickener, a chelating agent, a emollient, and a preservative.

[0026] In some embodiments of the present invention, the dosage form of the cosmetic includes lotion, serum, cream, or mask.

[0027] Compared with the prior art, the beneficial effects of the present invention are as follows: (1) The formulation of the composition of the present invention contains elastin, fibronectin, milk protein and agarwood extract. Through such a unique formulation combination, the components have a good synergistic effect, which solves the problem of incomplete target of single component action; it can promote the secretion of glycoprotein molecules such as hyaluronic acid, inhibit the decomposition of hyaluronic acid, exert moisturizing effect through different pathways, increase the moisture content of the epidermis and dermis, and maintain the skin's hydrated state; at the same time, it can also stimulate the production of collagen, resist physiological aging, smooth the skin to achieve anti-wrinkle effect, and delay skin aging; in addition, the production and increase of elastic fibers can effectively improve skin elasticity, thereby achieving a firming effect; furthermore, the anti-inflammatory and repairing effects of the ingredients themselves make the composition more gentle and suitable for consumers with different skin types.

[0028] (2) The composition of the present invention is mild and non-irritating, has good stability in actual cosmetic applications, and has multiple skin care effects such as firming, moisturizing and repairing, making it a promising candidate for application in cosmetic development. Detailed Implementation

[0029] The present invention will be further described in detail below through specific embodiments. Unless otherwise specified, the raw materials, reagents, or apparatus used in the embodiments can be obtained from conventional commercial sources or by existing technical methods. Unless otherwise specified, the experimental or testing methods are conventional methods in the art.

[0030] The sources of the raw materials used in this invention are as follows: Elastin, manufacturer: Guangzhou Nuowei Biotechnology Co., Ltd.; Fibronectin, manufacturer: Guangzhou Nuowei Biotechnology Co., Ltd.; Milk protein, manufacturer: Beijing Yanzhishan Technology Co., Ltd.; Agarwood extract, manufacturer: Guangzhou Nuowei Biotechnology Co., Ltd.; Palmitoyl pentapeptide-4, manufacturer: Guangzhou Zengcheng Miaosen Biotechnology Co., Ltd.; Retinol, manufacturer: BASF (China) Co., Ltd.; Methylsilanol mannouronate, manufacturer: EXSYMOL SAM; Purslane extract, manufacturer: HYUNDAI BIOLAND CO., LTD.

[0031] Example 1 A composition comprising the following components in parts by weight: 51 parts butanediol, 10 parts elastin, 10 parts fibronectin, 15 parts milk protein, 12 parts agarwood extract, and 2 parts 1,2-hexanediol.

[0032] The composition of this embodiment is prepared by mixing the components.

[0033] Example 2 A composition comprising the following components in parts by weight: 51 parts butylene glycol, 14 parts elastin, 7 parts fibronectin, 12 parts milk protein, 14 parts agarwood extract, and 2 parts 1,2-hexanediol.

[0034] The composition of this embodiment is prepared by mixing the components.

[0035] Example 3 A composition comprising the following components in parts by weight: 51 parts butanediol, 8 parts elastin, 13 parts fibronectin, 18 parts milk protein, 8 parts agarwood extract, and 2 parts 1,2-hexanediol.

[0036] The composition of this embodiment is prepared by mixing the components.

[0037] Comparative Example 1 The only difference from Example 1 is that it does not contain the active components (elastin, fibronectin, milk protein and agarwood extract), and is composed of the following components in parts by weight: 98 parts butylene glycol and 2 parts 1,2-hexanediol.

[0038] Comparative Example 2 The only difference from Example 1 is that it does not contain elastin and is composed of the following components in parts by weight: 51 parts butylene glycol, 13 parts fibronectin, 14 parts milk protein, 20 parts agarwood extract and 2 parts 1,2-hexanediol.

[0039] Comparative Example 3 The only difference from Example 1 is that it does not contain fibronectin and is composed of the following components in parts by weight: 51 parts butylene glycol, 16 parts elastin, 18 parts milk protein, 13 parts agarwood extract and 2 parts 1,2-hexanediol.

[0040] Comparative Example 4 The only difference from Example 1 is that it does not contain milk protein and is composed of the following components in parts by weight: 51 parts butylene glycol, 15 parts elastin, 17 parts fibronectin, 15 parts agarwood extract and 2 parts 1,2-hexanediol.

[0041] Comparative Example 5 The only difference from Example 1 is that it does not contain agarwood extract and is composed of the following components in parts by weight: 51 parts butylene glycol, 15 parts elastin, 15 parts fibronectin, 17 parts milk protein and 2 parts 1,2-hexanediol.

[0042] Comparative Example 6 The only difference from Example 1 is that it contains only one active component (elastin), which consists of the following components in parts by mass: 51 parts butanediol, 47 parts elastin and 2 parts 1,2-hexanediol.

[0043] Comparative Example 7 The only difference from Example 1 is that it contains only one active component (fibronectin), which consists of the following components in parts by mass: 51 parts of butanediol, 47 parts of fibronectin and 2 parts of 1,2-hexanediol.

[0044] Comparative Example 8 The only difference from Example 1 is that it contains only one active component (milk protein), which consists of the following components in parts by mass: 51 parts butanediol, 47 parts milk protein and 2 parts 1,2-hexanediol.

[0045] Comparative Example 9 The only difference from Example 1 is that it contains only one active component (agarwood extract), which is composed of the following components in parts by weight: 51 parts of butylene glycol, 47 parts of agarwood extract and 2 parts of 1,2-hexanediol.

[0046] Comparative Example 10 The only difference from Example 1 is that elastin is replaced with an equal amount of palmitoyl pentapeptide-4.

[0047] Comparative Example 11 The only difference from Example 1 is that fibronectin is replaced with an equal amount of retinol.

[0048] Comparative Example 12 The only difference from Example 1 is that milk protein is replaced in equal amounts with methylsilanol mannouronate.

[0049] Comparative Example 13 The only difference from Example 1 is that the agarwood extract is replaced with an equal amount of purslane extract.

[0050] Application Examples 1-3 Application Examples 1-3 each provide an essence, the specific formula of which is shown in Table 1. The ingredient content in Table 1 is expressed as a percentage by weight.

[0051] Table 1. Serum formulations from Application Examples 1-3

[0052] The preparation method of the serum using Examples 1-3 includes the following steps: (1) Heat the A phase raw material to 85°C, stir and disperse it evenly to obtain mixture one; (2) Heat the B phase raw material to 75°C, stir and disperse it evenly to obtain mixture II; (3) Add mixture 2 to mixture 1, stir homogenously, and cool to 60°C after stirring evenly to obtain mixture 3; (4) Preheat and dissolve the C phase raw material, then add it to the mixture three, stir evenly and continue to cool down. After cooling down to 45°C, add the D phase raw material and stir to disperse evenly.

[0053] Application Comparative Examples 1-13 The only difference from Application Example 1 is that in Application Examples 1-13, the composition of Example 1 was replaced in equal amounts with the composition of Comparative Examples 1-13.

[0054] Experimental Example 1: Stability Assessment Experimental Methods: High and low temperature stability tests were conducted. 100g of the essence sample was placed in a transparent PET bottle and subjected to temperatures of 5℃, 25℃, and 40℃ respectively. After 30 days, the sample was removed and allowed to return to room temperature (25±5℃). The condition was observed and recorded. Additionally, a temperature cycling test was performed between -15℃ and 5℃ to 48℃. The sample in the transparent PET bottle was first placed in a -15℃ refrigerator for 48 hours, then removed and placed in a 5℃ refrigerator for 48 hours, and finally removed and placed in a 48℃ oven for 48 hours, completing one cycle. This process was repeated five times, i.e., after 30 days, the sample was removed and allowed to return to room temperature. The condition of the sample was observed and recorded.

[0055] Results Analysis: If the sample removed after 30 days shows no significant difference in its state after returning to room temperature compared to before the test, it indicates that it has passed the high and low temperature stability test; otherwise, it has failed. The same method applies to the cyclic test: if the removed sample shows no significant difference in its state after returning to room temperature compared to before the test, it indicates that it has passed the cyclic test; otherwise, it has failed.

[0056] The experimental results are shown in Table 2 below.

[0057] Table 2. Results of high and low temperature stability of the serum in Application Examples 1-3 and Comparative Examples 1-13

[0058] As can be seen from the results of the high and low temperature stability tests in Table 2, all samples of the application examples and application comparison examples passed the stability test, indicating that the composition of the present invention, which combines firming, moisturizing and repairing properties, has good stability in cosmetic formulations. This is also the basis for the composition to be widely used in cosmetic development.

[0059] Experimental Example 2: Safety Assessment Test substances: the serums used in Examples 1-3 and Comparative Examples 1-13 of this invention.

[0060] Volunteer selection: Healthy individuals aged 18-35 years who voluntarily participated in and completed the trial as required, and signed an informed consent form. All unsuitable trial conditions were excluded. To ensure 30 valid data points per group, a total of 480 participants were included.

[0061] Experimental Methods: Every evening after cleansing, volunteers applied an appropriate amount of the product to the test side (both sides of the face were randomly divided into test and control sides; the test side used the product, while the control side did not use any product) of their facial skin, gently massaging with their fingertips and then lightly pressing to promote product absorption. The product was used once every evening for a 4-week testing period. Follow-up was conducted three times: before the study (day 0), 1 week after product use, and 4 weeks afterward. Researchers carefully observed and recorded the condition of the test side of the participants' faces. All adverse skin reactions occurring during the period, such as redness, stinging, itching, and burning, were recorded. Participants were required to report any serious adverse reactions immediately.

[0062] Results Analysis: Adverse skin reactions were graded according to the 2015 edition of the "Cosmetic Safety Technical Specifications" issued by the National Medical Products Administration, which specifies the grading of adverse skin reactions in human trials. Grade 0 (no skin reaction); Grade 1 (light red patches on the skin); Grade 2 (redness, infiltration, and papules on the skin); Grade 3 (redness, edema, papules, and vesicles on the skin); Grade 4 (redness, edema, and bullae on the skin).

[0063] The severity of adverse reactions is judged according to the following three-level criteria: Mild—tolerable to the subjects, requiring no special treatment, and does not affect the subjects' normal life; Moderate – Subjects find it difficult to tolerate, requiring discontinuation of the product or special treatment, affecting their normal lives; Severe – Interferes with normal life; product use must be stopped immediately or emergency treatment should be given.

[0064] The experimental results are shown in Table 3 below.

[0065] Table 3. Safety assessment results of the serums in application examples 1-3 and comparative examples 1-13.

[0066] According to the safety assessment results in Table 3, during the 4-week testing period, the composition of Comparative Example 1, which contained only butylene glycol and 1,2-hexanediol, showed that some volunteers in this group experienced Grade 1 adverse reactions. This indicates that the addition of elastin, fibronectin, milk protein, and agarwood extract can improve the mildness of the composition to a certain extent.

[0067] Comparing the results of Comparative Examples 2-5 and 6-9, it was found that the serum in Comparative Example 5, which lacked agarwood extract, showed Grade 1 adverse reactions, while the serums lacking elastin, fibronectin, and milk protein did not show any adverse reactions. Meanwhile, the serum in Comparative Example 9, which contained only agarwood extract, showed no adverse reactions, while the serums containing only any other active ingredient showed varying numbers of Grade 1 adverse reactions. This indicates that the addition of agarwood extract to the composition improved its mildness to some extent and reduced the probability of adverse reactions.

[0068] The results from comparative examples 10-13 show that Grade 1 adverse reactions occurred when retinol was used to replace fibronectin and when purslane extract was used to replace agarwood extract. This indicates that the mildness of the replaced compositions decreased compared to those in examples 1-3. In summary, the combination of elastin, fibronectin, milk protein, and agarwood extract selected in this invention is mild in nature, and no adverse reactions were observed during use, laying the foundation for the widespread application of this composition.

[0069] Experiment Example 3: Moisturizing Efficacy Test Test substance: The essence of the present invention applied in Examples 1-3 and Comparative Examples 2-13.

[0070] Participants: Healthy individuals aged 18-35 years who voluntarily participated and were able to complete the trial as required, maintained a regular lifestyle, avoided ultraviolet radiation, and did not use anti-allergy medications throughout the trial observation period, and signed an informed consent form. All unsuitable trial conditions were excluded, and a total of 450 participants were included to ensure 30 valid data points per group.

[0071] Experimental Method: After cleansing their skin morning and evening, volunteers applied an appropriate amount of the sample to their facial skin, gently massaging it in with their fingertips and then lightly pressing to promote absorption. The test period was 7 days. On the first day (D0) and the seventh day (D7) after use, under conditions of 21±1℃ and 50±10% humidity, volunteers cleansed their faces and waited 20 minutes before testing the skin's moisture content to comprehensively evaluate the serum's moisturizing effect on human skin.

[0072] Results Analysis: The rate of change in skin moisture content after using the test product was recorded compared to before use. Positive values ​​indicated increased moisture content and improved skin hydration, while negative values ​​indicated decreased moisture content and reduced skin hydration. The Shapiro-Wilk test was used to test the significance of the improvement values ​​obtained before use (D0) and on the seventh day of use (D7) according to the normal distribution. If the distribution was normal, a paired t-test was performed, with a significance level of α set at 0.05; if the distribution was non-normal, a Wilcoxon test was performed, with a significance level of α set at 0.05. A significant improvement in the change in skin stratum corneum moisture content before and after product use (P < 0.05) was considered indicative of the product's moisturizing effect; otherwise, the product was considered not to have a moisturizing effect.

[0073] The experimental results are shown in Table 4 below.

[0074] Table 4 Results of skin moisture content

[0075] As shown in Table 4, after 7 days of use, the skin moisture content of the serums in Examples 1-3 was significantly improved. The effect was better than that of Comparative Examples 10-13, which replaced one of the active ingredients with similar active ingredients. Even the results of Comparative Examples 10 and 12 showed significant differences, indicating that the composition has a moisturizing effect, but its effect on improving skin moisture content is not as good as that of Application Examples 1-3 containing the composition of the present invention.

[0076] Furthermore, the moisturizing effect of compositions lacking any one of the active ingredients or containing only one active ingredient is far inferior to that of compositions in Examples 1-3, and there is no significant difference before and after use, indicating that the serum with this composition has no moisturizing effect. In summary, the composition of the four active ingredients selected in this invention—elastin, fibronectin, milk protein, and agarwood extract—has a synergistic effect and can better enhance the moisturizing effect.

[0077] Experiment 4: Repair Efficacy Test Test substance: The essence of the present invention applied in Examples 1-3 and Comparative Examples 2-13.

[0078] Subjects: 450 volunteers from the same trial example 3.

[0079] Experimental Method: After cleansing their skin morning and evening, volunteers applied an appropriate amount of the sample to their facial skin, gently massaging it in with their fingertips and then lightly pressing to promote absorption. The test period was 7 days. On the first day (D0) and the seventh day (D7) after use, under conditions of 21±1℃ and 50±10% humidity, volunteers cleansed their faces and waited 20 minutes before measuring the transepidermal water loss (TEWL) value of their facial skin to comprehensively evaluate the repair efficacy of the serum on human skin.

[0080] Results Analysis: The rate of change in skin moisture loss rate after using the test product was recorded compared to before use. Positive values ​​indicated increased skin moisture loss rate and weakened skin barrier function, while negative values ​​indicated decreased skin moisture loss rate and enhanced skin barrier function. The Shapiro-Wilk Test was used to test the normality of the improvement values ​​measured before use (D0) and on day 7 (D7). If a normal distribution was observed, a paired t-test was performed, with a significance level of α set at 0.05. If a non-normal distribution was observed, a Wilcoxon test was performed, with a significance level of α set at 0.05. A significant improvement in any transdermal moisture loss rate before and after product use (P < 0.05) was considered indicative of the product's repair efficacy; otherwise, the product was deemed not to have a repair efficacy.

[0081] The test results are shown in Table 5 below.

[0082] Table 5. TEWL Value Analysis Results

[0083] As shown in Table 5, the transdermal water loss (TEWL) of samples from Application Examples 1-3 was significantly reduced after 7 days of use. Although there were also significant differences in Application Examples 11 and 13 when one of the active ingredients was replaced, the percentage reduction was still smaller than that of Application Examples 1-3. This indicates that the combination of the four active ingredients selected in this invention—elastin, fibronectin, milk protein, and agarwood extract—can effectively reduce transdermal water loss. The repair effect was affected by the absence of any one of these ingredients, indicating that the combination of these four ingredients has a synergistic effect.

[0084] Furthermore, the results from Application Examples 1-3 show that adjusting the proportion of active ingredients in the composition has a certain impact on its repair effect. Application Example 1 showed the most significant effect in reducing transdermal water loss. In summary, the composition of elastin, fibronectin, milk protein, and agarwood extract selected in this invention has repairing effects.

[0085] Experiment 5: Firming Efficacy Test Test substance: The essence of the present invention applied in Examples 1-3 and Comparative Examples 2-13.

[0086] Subjects: 450 volunteers from the same trial example 3.

[0087] Test Method: Volunteers applied an appropriate amount of the sample to their face after cleansing morning and evening, gently massaging with their fingertips and then lightly pressing to promote product absorption. The test period was 28 days. Skin elasticity (R2) of the target area was measured using a Cutometer® MPA580 from COURAGE+KHAZAKA (Germany). Three measurements were taken, and the average value was recorded. Follow-up was conducted at week 0, week 1, and week 4 after product use. After cleansing their faces, subjects sat quietly for 20 minutes under relatively constant environmental conditions (room temperature 21±1℃, humidity 50±5%) before the test.

[0088] Results Analysis: The effect of the product on skin elasticity was assessed by comparing the changes in skin elasticity (R²) before and after product use, indirectly evaluating the product's anti-wrinkle efficacy. A higher R² value (closer to 1) indicates better skin elasticity in the test area. The Shapiro-Wilk Test was used to test the normality of the improvement values ​​measured before use (D0) and on day 7 (D7). If the distribution was normal, a paired t-test was performed, with a significance level of α set at 0.05. If the distribution was non-normal, a Wilcoxon test was performed, with a significance level of α set at 0.05. A significant improvement in the R² value at any viewing point before and after product use (P < 0.05) was considered indicative of a firming effect; otherwise, the product was deemed not to have a firming effect.

[0089] The test results are shown in Table 6 below.

[0090] Table 6 Skin elasticity (R2) values

[0091] As shown in Table 6, after 28 days of use, the skin elasticity R2 value of samples from Examples 1-3 showed a significant increase compared to before use, indicating that the combination of the four active ingredients—elastin, fibronectin, milk protein, and agarwood extract—selected in this invention has a firming effect. However, after 28 days of use, samples from Comparative Examples 2-13 showed no significant difference except for Comparative Example 13. Even after 28 days of use, the skin elasticity R2 value of the serum from Comparative Example 13 changed by only 10.21%, which was not as significant as the change in Examples 1-3. This indicates that all four active ingredients selected in this invention are indispensable. Samples prepared with a composition lacking any one of the active ingredients or containing only one active ingredient do not show a significant effect on improving skin elasticity. Therefore, this invention demonstrates that the combination of the four active ingredients has a firming effect and a synergistic effect.

[0092] Experimental Example 6: Consumer Evaluation Test substance: The essence of the present invention applied in Examples 1-3 and Comparative Examples 2-13.

[0093] Subjects: 450 volunteers from the same trial example 3.

[0094] Experimental Methods: Volunteers applied an appropriate amount of product to their face after cleansing morning and evening, gently massaging with their fingertips and then lightly pressing to promote absorption. The product was used once in the morning and once in the evening for 7 days. A single-blind method was used. After the test period, volunteers evaluated their skin condition using a questionnaire to analyze the product's effectiveness.

[0095] Results analysis: A product is considered effective if the effectiveness rate of the evaluation dimensions is greater than or equal to 50%. Effectiveness rate = (Number of obvious cases + Number of very obvious cases) / Total number of cases.

[0096] The results of the efficiency are shown in Table 7 below.

[0097] Table 7 Analysis of Product Evaluation Results

[0098] As shown in Table 7, consumer reviews indicate that the moisturizing and firming effects of the serums in Examples 1-3 are better than those in Comparative Examples 2-13. This is consistent with the moisturizing and firming test results of Examples 3 and 5. In summary, the four active ingredients selected in this invention—elastin, fibronectin, milk protein, and agarwood extract—provide a positive user experience while ensuring product efficacy, demonstrating promising application prospects.

[0099] The preferred embodiments of the present invention have been described in detail above, but the present invention is not limited to the embodiments described. Those skilled in the art can make various equivalent modifications or substitutions without departing from the spirit of the present invention, and these equivalent modifications or substitutions are all included within the scope defined by the claims of this application.

Claims

1. A composition, characterized in that, The composition comprises, by weight parts, the following components: 1-30 parts elastin, 1-30 parts fibronectin, 1-40 parts milk protein and 1-35 parts agarwood extract.

2. The composition according to claim 1, characterized in that, The composition comprises, by weight parts, the following components: 1-25 parts elastin, 1-25 parts fibronectin, 1-35 parts milk protein, and 1-30 parts agarwood extract.

3. The composition according to claim 2, characterized in that, The composition comprises, by weight parts, the following components: 8-14 parts elastin, 7-13 parts fibronectin, 12-18 parts milk protein and 8-14 parts agarwood extract.

4. The composition according to claim 1, characterized in that, The composition also includes a solvent.

5. The composition according to claim 4, characterized in that, The solvent includes at least one selected from butanediol, 1,2-hexanediol, 1,3-propanediol, propylene glycol, and 1,2-pentanediol.

6. A method for preparing the composition according to any one of claims 1-5, characterized in that, Includes the following steps: The components are mixed to obtain the composition.

7. A cosmetic product, characterized in that, The cosmetic product includes the composition according to any one of claims 1-5.

8. The cosmetic product according to claim 7, characterized in that, The composition has a mass content of 0.1%-10% in cosmetics.

9. The cosmetic product according to claim 7, characterized in that, The cosmetics also include at least one of thickeners, chelating agents, emollients, and preservatives.

10. The cosmetic product according to claim 7, characterized in that, The dosage forms of the cosmetics include lotions, serums, creams, or masks.

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