System device for inactivating iridovirus in aquaculture water and application method of system device

Abstract

The invention provides a system device for inactivating iridovirus in aquaculture water, the system device comprises a pump pool, a circulating pump, an ozone generator and a reaction kettle, the pump pool is an open pool structure, the circulating pump is arranged in the pump pool, the lower end of the reaction kettle is provided with a water inlet pipe, the circulating pump is connected with the water inlet pipe through a pipeline, and the ozone generator is connected with the water inlet pipe through a pipeline. The ozone generator is arranged on a pipeline through which the circulating pump is connected with the reaction kettle, a reaction barrel is arranged in the reaction kettle, a water storage area is formed between the reaction barrel and the inner wall of the reaction kettle, a fixing frame is arranged at the upper end in the reaction kettle, and a plurality of mounting rods are vertically mounted on the fixing frame; the mounting rods are uniformly distributed in the water storage area and the reaction barrel, and a plurality of ultraviolet emitting modules are mounted on the mounting rods. The method provided by the invention realizes thorough disinfection of microorganisms such as iridovirus and rapid degradation of nitrite by using an ultraviolet and ozone combined technology, and has the advantages of environmental protection, no residue, strong adaptability, low operation cost and the like.

Description

Technical Field

[0001] This invention relates to the field of aquaculture equipment technology, specifically to a system device and its application method for inactivating iridoviruses in aquaculture water. Background Technology

[0002] The recirculating aquaculture system (RAS) industry has broad prospects, but its large-scale promotion still faces two major technological bottlenecks: the challenge of pathogen control and the difficulty of water pollutant degradation. Iridovirus, one of the most serious pathogenic viruses in aquaculture, hosts a variety of freshwater and marine fish species, including carp, crucian carp, mandarin fish, and grouper. It is characterized by wide transmission routes, high pathogenicity, and high mortality. Once an outbreak occurs in an ARS system, it can spread rapidly through the recirculating water, leading to large-scale fish deaths and significant economic losses. Traditional chemical disinfection methods, such as disinfectants and antibiotics, can suppress pathogens in the short term, but they easily produce drug residues, disrupt the aquatic microecological balance, reduce the immunity of farmed organisms, and may induce drug resistance in pathogens, creating a vicious cycle of "disinfection-drug resistance-re-disinfection," which does not meet the requirements of green aquaculture development.

[0003] On the other hand, nitrite, as a major nitrogen metabolic pollutant in recirculating aquaculture systems, has long been a key factor restricting the industry's development. Nitrogenous waste excreted by farmed organisms undergoes ammoniation to produce ammonia nitrogen, which is then converted to nitrite through nitrification. Nitrite is toxic and can be absorbed into the bloodstream through the gills of farmed organisms, impairing the oxygen-carrying capacity of hemoglobin and leading to asphyxiation and death. It also inhibits the growth, development, and immunity of farmed organisms, increasing the risk of disease infection. Traditional nitrite degradation technologies, such as biofilters and chemical reduction, suffer from low degradation efficiency, demanding reaction conditions, and a tendency to generate secondary pollution, making it difficult to meet the high-load, high-turnover water purification requirements of recirculating aquaculture systems. In high-density recirculating aquaculture scenarios, nitrite concentrations often easily exceed the safe threshold (0.1 mg / L), becoming a significant factor restricting the increase in stocking density and improving aquaculture efficiency.

[0004] Currently, there are some technologies or devices on the market that use a combination of ozone and ultraviolet light to kill microorganisms. For example, the utility model patent with publication number CN 205635208 U provides a modular device for killing microorganisms by combining ozone and ultraviolet light in aquaculture. By setting up a combination of ozone pool module and ultraviolet light pool module, it significantly improves the killing ability of microorganisms such as bacteria, viruses, fungi, microalgae, parasites, insect eggs and spores in aquaculture water. However, the patent does not provide any explanation or evidence that the combination can kill iridovirus. Therefore, there is no evidence to support whether it is effective in killing iridovirus. Moreover, the combination does not mention that it can rapidly degrade nitrite. Summary of the Invention

[0005] The purpose of this invention is to provide a system device for inactivating iridoviruses in aquaculture water. It utilizes a combination of ultraviolet light and ozone technology to thoroughly eliminate iridoviruses and other microorganisms and rapidly degrade nitrites, resulting in an environmentally friendly and residue-free system that simultaneously improves the ecological environment of aquaculture water.

[0006] The technical solution adopted in this invention is as follows: A system for inactivating iridovirus in aquaculture water includes a pump pool, a circulating pump, an ozone generator, and a reaction vessel. The pump pool is an open water tank structure. The circulating pump is located inside the pump pool. The lower end of the reaction vessel is provided with a water inlet pipe, and the circulating pump is connected to the water inlet pipe via a pipeline. The ozone generator is located on the pipeline connecting the circulating pump and the reaction vessel. A reaction tank is provided inside the reaction vessel. The reaction tank has a cylindrical structure, closed at the lower end and open at the upper end. A water storage area is formed between the reaction tank and the inner wall of the reaction vessel. A fixing frame is provided at the upper end of the inside of the reaction vessel. The fixing frame is higher than the top of the reaction tank and is cross-shaped. Multiple mounting rods are vertically installed on the fixing frame. The mounting rods are evenly distributed in the water storage area and inside the reaction tank. Multiple ultraviolet emitting modules are installed on the mounting rods.

[0007] Furthermore, the upper end of the reactor has an arc-shaped structure, and an opening is provided at the top of the reactor, with an exhaust device installed at the upper end of the opening.

[0008] Furthermore, the reaction vessel is mounted in the center of the reactor via a frame.

[0009] Furthermore, the ultraviolet emission module uses ultraviolet lamps with a wavelength of 254nm, which can be arranged horizontally or vertically.

[0010] Furthermore, a reflective film is affixed to the inner wall of the reactor and the reaction vessel.

[0011] Furthermore, a water outlet pipe is provided at the lower end of the reaction tank, which extends through the side wall of the reaction tank and the reactor to the outside of the reactor and connects to other external processing equipment.

[0012] Furthermore, a water level sensor is also installed inside the reactor. The water level sensor is mounted on a fixed frame and is electrically connected to the circulating pump and the ozone generator.

[0013] A method for applying a system device for inactivating iridovirus in aquaculture water as described above includes the following steps: ① Introduce the aquaculture water containing iridovirus into the pump pool to keep the water in a continuous flow state; ② The circulation pump is started to pump the water in the pump pool into the reactor. At the same time, the ozone generator is started to inject ozone into the water. The ozone concentration is controlled at 0.3-0.5 mg / L to ensure that the ozone is evenly dispersed throughout the water. ③ Water enters the water storage area inside the reactor through the inlet pipe. As water continues to enter, it fills the water storage area and overflows from the top of the reactor into the reactor. Ultraviolet lamps inside and outside the reactor simultaneously irradiate the flowing water sample. The ultraviolet light is in the UVC band with an irradiation wavelength of 254nm and an irradiation intensity of 20-40mW / cm². The water enters the reactor and then flows out through the outlet pipe. The entire operation time is controlled within 10-20 minutes. ④ The treated water flows out of the reaction tank to other external treatment equipment.

[0014] Furthermore, the ultraviolet intensity is 30 mW / cm², the ozone concentration is 0.3 mg / L, and the ultraviolet irradiation time is 20 minutes.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention utilizes a combination of ultraviolet light and ozone to achieve a dual-stage synergistic disinfection mechanism of "inactivation + degradation." It not only inactivates viruses but also degrades viral nucleic acid fragments, fundamentally eliminating the risk of secondary infection caused by viral residues. This solves the problem of traditional technologies that "only inactivate but do not degrade," eliminating the need for chemical disinfectants and avoiding the harm of drug residues to aquatic organisms and the aquatic ecosystem. At the same time, it also degrades nitrite, simultaneously improving the aquatic ecological environment and reducing the risks of aquaculture caused by water quality deterioration. It has advantages such as being environmentally friendly and residue-free, highly adaptable, and having low operating costs. Attached Figure Description

[0016] Appendix Figure 1 This is a schematic diagram of the system device for inactivating iridovirus in aquaculture water as described in this invention. Detailed Implementation

[0017] This invention provides a system apparatus and method for inactivating iridovirus in aquaculture water. To make the objectives, technical solutions, and effects of this invention clearer and more explicit, the invention is further described in detail below. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

[0018] As attached Figure 1As shown, a system for inactivating iridovirus in aquaculture water includes a pump tank 1, a circulating pump 2, an ozone generator 3, and a reaction vessel 4. The pump tank 1 is an open pool structure used to hold the water to be purified. The aquaculture water, after preliminary physical treatment such as vertical flow sedimentation, microfiltration, and protein separation, flows into the pump tank 1. The circulating pump 2 is located inside the pump tank 1. An inlet pipe 41 is installed at the lower end of the reaction vessel 4. The circulating pump 2 is connected to the inlet pipe 41 via a pipe and is used to pump water from the pump tank 1 into the reaction vessel 4. The ozone generator 3 is located on the pipe connecting the circulating pump 2 and the reaction vessel 4 and is used to continuously supply ozone to the water, with the ozone concentration controlled at 0.3–0.5%. mg / L ozone is thoroughly mixed with the water to be treated and then introduced into reactor 4. Ozone can rapidly oxidize the protein shell of viruses, destroying their structural integrity and causing them to lose their ability to attach to host cells. The main principle is that since ozone molecules are composed of three oxygen atoms, they are extremely unstable. In water, they will rapidly decompose into a stable oxygen molecule and a monatomic oxygen with extremely strong oxidizing properties. This monatomic oxygen can oxidize the cell wall, lipids on the cell membrane, enzyme system, and internal genetic material of microorganisms, causing cell structure rupture and leakage of contents, thereby killing microorganisms. At the same time, ozone can also effectively oxidize colored dissolved organic matter (such as the yellow substance produced by fish excrement) and inorganic matter (such as nitrite) in water.

[0019] The reactor 4 has a cylindrical structure with a certain strength. A water inlet pipe 41 is installed at the lower end of the reactor 4, through which water from the pump tank 1 enters the reactor 4. The upper end of the reactor 4 has an arc-shaped structure, and an opening (not shown in the figure) is provided at the top of the reactor 4. An exhaust device 42 is installed at the upper end of the opening to discharge gases from the reactor 4. By removing the exhaust device 42, operators can also enter the reactor 4 through the opening to perform inspection or maintenance work. A reaction tank 43 is installed inside the reactor 4. The reaction tank 43 is mounted in the center of the reactor 4 via a frame. The reaction tank 43 has a cylindrical structure, closed at the bottom and open at the top. A water storage area is formed between the reaction tank 43 and the inner wall of the reactor 4. As water continuously enters the reactor 4, after the water storage area is filled, it overflows from the top of the reaction tank 43 into the reaction tank 43. A fixing frame 44 is installed at the upper part of the reactor 4, higher than the top of the reaction tank 43. The fixing frame 44 is cross-shaped and fixes the reaction tank 43. Multiple mounting rods 441 are vertically installed on the frame 44. The mounting rods 441 are evenly distributed in the water storage area and the reaction tank 43. Multiple ultraviolet emitting modules 45 are installed on the mounting rods 441. The ultraviolet emitting modules 45 use ultraviolet lamps with a wavelength of 254nm. The ultraviolet lamps can be set horizontally or vertically. In order to enhance the irradiation effect of ultraviolet light, a reflective film (not shown in the figure) is attached to the inner wall of the reaction vessel 4 and the reaction tank 43. The reflective film can reflect the scattered ultraviolet light back into the water flow, which greatly improves the utilization rate and irradiation uniformity of ultraviolet light and ensures the sterilization effect. Ultraviolet light, especially in the wavelength band of 254nm, can penetrate the cell wall of microorganisms (bacteria, viruses, protozoa) and be absorbed by the DNA and RNA in their cell nuclei. The absorbed energy will destroy the molecular structure of nucleic acids, especially causing adjacent thymine to form dimers. This is equivalent to destroying the "genetic code" and "replication instructions" of microorganisms, causing them to be unable to reproduce normally and subsequently die. Ultraviolet light does not change the chemical properties of water and does not produce disinfection byproducts.A water outlet pipe 46 is provided at the lower end of the reaction tank 43. The water outlet pipe 46 passes through the side wall of the reaction tank 43 and the reaction vessel 4, extends to the outside of the reaction vessel 4, and connects to other external treatment equipment. A water level sensor 47 is also provided inside the reaction vessel 4. The water level sensor 47 is mounted on the fixed frame 44 and is electrically connected to the circulation pump 2 and the ozone generator 3. Since the height of the water level sensor 48 is higher than the top of the reaction tank 43, when the water level in the reaction vessel 4 reaches the position of the water level sensor 47, it indicates that the water level in the reaction vessel 4 is too high. The water level sensor 47 sends a signal to the circulation pump 2 to reduce the operating power and reduce the water intake. At the same time, the ozone generator 3 reduces the ozone injection. The design of the reaction tank 43 can effectively increase the distance of water flow and increase the time of ultraviolet lamp irradiation. The entire operation time from the water entering the reaction vessel 4 to flowing out from the water outlet pipe 46 is controlled within 20 minutes, that is, the time for the water to receive ultraviolet irradiation is controlled within 20 minutes. The water running time in the reaction vessel 4 can be controlled by the circulation pump 2. ;

[0020] The application method of the device provided above is as follows: 1. Introduce the aquaculture water containing iridovirus into pump pool 1 to keep the water in a continuous flow state; 2. The circulation pump 2 is started to pump the water in the pump pool 1 into the reaction vessel 4. At the same time, the ozone generator 3 is started to inject ozone into the water. The ozone concentration is controlled at 0.3–0.5 mg / L to ensure that the ozone is evenly dispersed throughout the water. 3. Water enters the water storage area inside the reactor 4 through the inlet pipe. As water continues to enter, after the water storage area is full, it overflows from the top of the reactor 43 into the reactor 43. The ultraviolet lamps inside and outside the reactor 43 simultaneously irradiate the flowing water sample. The ultraviolet light is in the UVC band, the wavelength of the ultraviolet module is 254nm, the irradiation intensity is 0.2-0.4mW / cm², and the ultraviolet irradiation time is 10-20 minutes. 4. The treated water flows out from reaction tank 43 to other external treatment equipment.

[0021] The technical solution of the present invention will be further explained and described below through specific embodiments.

[0022] To verify the effectiveness of the method provided by this invention, the breeding process was conducted in a laboratory, and the experiment was carried out according to the following standards.

[0023] (a) Experimental materials and equipment: 1.1 Virus-containing water samples: Aquaculture water samples from a large-scale recirculating aquaculture base that were confirmed to be infected with iridovirus were selected, totaling approximately 120 catties. The virus concentration was pre-detected by quantitative real-time PCR and met the positive standard (Ct value < 37) to ensure the validity and representativeness of the experimental samples. After collection, the water samples were placed in sterile containers and stored at room temperature (28℃) to avoid direct sunlight and ensure the stability of virus activity. The nitrite concentration was 0.3 mg / L.

[0024] 1.2 Disinfection equipment: The system device provided above for inactivating iridovirus in aquaculture water (hereinafter referred to as the inactivation system).

[0025] 1.3 Detection Instruments and Reagents: StepOnePlus fluorescence PCR instrument (ABI, USA), with high sensitivity and specificity, can accurately detect viral nucleic acid fragments; Iridovirus-specific detection kit (commercially available, sensitivity ≤10 copies / μL), can specifically detect iridovirus and non-viable viral DNA fragments, ensuring the accuracy of detection results; Nitrite detection kit (commercially available, conforming to GB / T 13889-2017 "Determination of Nitrite Nitrogen in Water - Spectrophotometric Method" standard), with a detection accuracy of 0.01 mg / L; high-speed refrigerated centrifuge (speed ≥12000 rpm), constant temperature incubator, ultraviolet spectrophotometer, and other experimental equipment.

[0026] (II) Experimental Methods 2.1 Water Sample Grouping and Treatment Three parallel experiments were conducted on the water sample, with each experiment performed using the same procedures: Take 70 catties of water containing the virus, and extract 1.2 liters as an untreated control sample; The remaining 67.6 catties was introduced into the inactivation system, and after 10 minutes of treatment, 1.2 liters were extracted. The equipment ran continuously for 20 minutes, extracting 1.2 liters. Collect the remaining approximately 50 catties of water sample, place it in a sterile container, and let it stand at room temperature (28℃) in the dark for 24 hours. Then, extract 1.2 liters of water sample. To verify the reliability of the equipment, the above experiment was repeated three times, with the sample size and processing parameters remaining consistent each time.

[0027] 2.2 Virus Detection Process (1) Sample pretreatment: Nucleic acid extraction was performed on water samples of each group according to the instructions of the iridovirus detection kit. 1 mL of water sample was added to a centrifuge tube, nucleic acid extraction reagent was added, and the mixture was vortexed and then centrifuged at 12000 rpm for 15 minutes. The supernatant was collected as a nucleic acid template and stored in a -20℃ refrigerator for later use. (2) PCR reaction system preparation: The total volume of the reaction system is 20 μL, including: 2 μL nucleic acid template, 10 μL primer mixture, 1 μL enzyme reagent, and 7 μL enzyme-free water; all reagents are prepared precisely according to the kit instructions to avoid contamination; (3) PCR reaction conditions: The StepOnePlus real-time PCR instrument was used for detection. The reaction conditions were: 95℃ pre-denaturation for 3 minutes; 95℃ denaturation for 15 seconds, 58℃ annealing for 30 seconds, 72℃ extension for 30 seconds, for a total of 40 cycles; and finally 72℃ final extension for 5 minutes.

[0028] (4) Result judgment criteria: According to the kit instructions and industry standards, a Ct value <37 is judged as positive, indicating that there is a virus or viral DNA fragment in the water sample; a Ct value ≥37 or no obvious amplification curve is judged as negative, indicating that there is no virus or viral DNA fragment residue in the water sample.

[0029] 2.3 Detection Procedure for Nitrite Degradation Capacity The above-mentioned aquaculture water body was selected, and the initial nitrite concentration was found to be 0.3 mg / L. The inactivation system was started, and three parallel water samples were collected from different areas of the water body after 10 minutes and 20 minutes of equipment operation. The concentration was detected by using a nitrite detection kit in combination with an ultraviolet spectrophotometer, and the nitrite degradation amount and degradation rate were calculated.

[0030] Data statistics and analysis: SPSS 22.0 statistical software was used to analyze the experimental data. Quantitative data are expressed as mean ± standard deviation. One-way ANOVA was used to compare the significance of differences between groups. P < 0.05 was considered statistically significant. Example 1

[0031] Experimental conditions: UV: 30mW / cm²; Ozone: 0mg / L.

[0032] This example is used to evaluate the contribution of ultraviolet radiation alone. As a control, the following are the results of three parallel experiments: Results of the first experiment:

[0033] Results of the second experiment:

[0034] Results of the third experiment:

[0035] Example 2

[0036] Experimental conditions: Ozone-only treatment group (Ozone: 0.3 mg / L; UV: 0 mW / cm²) This example is used to evaluate the contribution of ozone oxidation alone. As a control, the following are the results of three parallel experiments: Results of the first experiment:

[0037] Results of the second experiment:

[0038] Results of the third experiment: Example 3

[0039] Experimental conditions: UV-ozone combined treatment group (UV: 30mW / cm² + Ozone: 0.3mg / L) This example demonstrates the combined disinfection and purification effect of using ultraviolet light and ozone. The following are the results of three parallel experiments: Results of the first experiment:

[0040] Results of the second experiment:

[0041] Results of the third experiment:

[0042] Experimental results: The results of three parallel experiments were completely consistent, verifying the stability and reliability of the inactivation system. The three experiments showed that ultraviolet light alone had limited inactivation effect on the virus (Ct value 31), almost no degradation of nitrite (6.5%), and the virus remained after standing. Ozone alone could partially degrade nitrite (38%), but virus inactivation was incomplete (Ct value 31.5), and the virus was not cleared after standing. After 20 minutes of treatment with the combined ultraviolet-ozone treatment group, the viral load significantly decreased (Ct value > 33), the nitrite degradation rate stabilized in the 64%-66% range, and the virus was completely cleared after standing (negative). Therefore, it is evident that the combination of ultraviolet intensity 30 mW / cm² and ozone dosage 0.3... The combination of mg / L achieves a unified approach that cannot be achieved by a single technology, namely, complete virus elimination and efficient nitrite degradation. Using data from the first experiment as an example, the immediate detection results of different treatment groups are as follows: The untreated control sample (number 3-1-1) had a Ct value of 27.824, showing a strong positive result, confirming that the water sample used in the experiment contained highly active iridovirus, thus establishing the experimental basis; the 10-minute treatment group (number 3-1-2) had a Ct value of 32.1, still positive, but the Ct value was significantly higher than the control sample (P < 0.05), indicating that the viral structure had been initially damaged, but the viral DNA fragments remained intact; 20 The Ct value of the 1-minute treatment group (number 3-1-3) was 33.16, which was positive. The Ct value further increased (P<0.05), indicating that the destructive effect on the viral capsid and nucleic acid chain was enhanced, but the viral core and residual DNA fragments could still be detected. After standing for 24 hours, the test results showed that the water sample of number 3-1-4 (24-hour standing group) was negative after PCR testing. There was no obvious amplification curve and the Ct value was ≥40. This indicates that after ultraviolet + ozone treatment, the residual ozone and ultraviolet damage effects continued to work, and the viral core and DNA fragments were completely degraded within 24 hours, achieving complete elimination of the virus.

[0043] The results showed that the technology achieved a 99.9% disinfect rate against iridovirus, inactivating the virus after 20 minutes of treatment, and completely degrading the viral core and DNA fragments after 24 hours of standing at room temperature (negative PCR test). The nitrite degradation rate was over 40 g / h, which translates to a reduction in nitrite concentration from 0.3 mg / L to 0.1 mg / L within 2 hours. Based on a flow rate of 200 cubic meters per hour, this equates to a degradation rate of 40 g of nitrite per hour, demonstrating a significant water purification effect.

[0044] Analysis of experimental principle: (1) When ultraviolet light irradiates the water at its optimal wavelength of 254 nm, the photon energy is absorbed by the viral nucleic acid, causing DNA double-strand breaks or cross-linking, destroying the integrity of the virus's genetic material, and blocking its replication and reproduction capabilities. At the same time, the high concentration of ozone injected into the water, reaching 0.3 mg / L, rapidly oxidizes the viral protein coat with its strong oxidizing properties, destroying its structural integrity and causing it to lose its ability to attach to host cells. At this stage, although the virus has been inactivated, some nucleic acid fragments or cellular debris have not been completely decomposed, so the PCR test is still positive.

[0045] (2) Degradation stage (within 24 hours after standing): After the equipment stops operating, the high concentration of ozone remaining in the water does not dissipate immediately, but continues to exert an oxidizing effect, further decomposing the nucleic acid fragments and protein residues of the virus; at the same time, the breaking effect of ultraviolet light on the nucleic acid chain is cumulative, and the damaged DNA fragments are difficult to repair. Under the continuous oxidation of ozone, they are gradually degraded into small molecules and eventually decomposed and utilized by the microorganisms in the water.

[0046] (3) Ultraviolet light enhances the decomposition of ozone to generate a large number of hydroxyl radicals. The oxidation-reduction potential of hydroxyl radicals is as high as 2.80V, which is much higher than that of ozone itself. It has stronger oxidation activity and can directly attack the chemical bonds of nitrite, oxidizing and decomposing it into nitrate. By optimizing the matching parameters of ozone concentration and ultraviolet irradiation intensity, ozone and water are efficiently mixed to ensure that hydroxyl radicals are evenly distributed in the water, thereby achieving rapid degradation of nitrite.

[0047] This invention utilizes a combination of ultraviolet light and ozone to achieve a two-stage synergistic disinfection mechanism of "inactivation + degradation," completely solving the pain point of traditional technologies that "only inactivate, but do not degrade." By employing a direct oxidation pathway of "ultraviolet light enhancing ozone decomposition to generate hydroxyl radicals," it overcomes the bottleneck in nitrite degradation efficiency, achieving integrated "disinfection + degradation" functions. Compared to single ultraviolet light technology, it offers a wider disinfection spectrum; compared to single ozone technology, it reduces ozone usage by 40%, lowering costs and toxicity; compared to biological filters, it increases nitrite degradation rate by 5-8 times, exhibits stronger resistance to environmental fluctuations, and boasts advantages such as environmental friendliness, no residue, strong adaptability, and low operating costs, aligning with the "green, efficient, and large-scale" development trend of the recirculating aquaculture industry.

[0048] This invention features a simple structure and convenient operation. Compared to traditional biological filter technology, which requires regular replacement of filter media and maintenance of microbial activity, resulting in high maintenance costs, the inactivation system provided by this invention has a simple structure, long service life of ultraviolet lamps and ozone generating modules (with lamps lasting over 20,000 hours), and requires only simple cleaning and parameter checks in daily use. Maintenance costs are reduced by more than 60% compared to traditional technologies. Combined with its high degradation efficiency, it can significantly reduce the frequency of water replacement, further reducing water and energy consumption and saving operating costs for aquaculture enterprises.

[0049] The above description is merely a preferred embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structural transformations made based on the description and drawings of the present invention, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of the present invention.

Claims

1. A system for inactivating iridovirus in an aquaculture water body comprising a pump tank, a circulation pump, an ozone generator and a reaction vessel, characterized in that: The pump pool is an open pool structure, the circulating pump is arranged in the pump pool, the lower end of the reaction kettle is provided with a water inlet pipe, the circulating pump is connected with the water inlet pipe through a pipeline, the ozone generator is arranged on the pipeline connecting the circulating pump and the reaction kettle, a reaction bucket is arranged in the reaction kettle, the reaction bucket is a cylindrical structure, the lower end of the reaction bucket is closed, and the upper end of the reaction bucket is open, a water storage area is formed between the reaction bucket and the inner wall of the reaction kettle, a fixing frame is arranged at the upper end of the reaction kettle, the fixing frame is higher than the top end of the reaction bucket, a plurality of mounting rods are vertically arranged on the fixing frame, the mounting rods are uniformly arranged in the water storage area and the reaction bucket, and a plurality of ultraviolet light emitting modules are arranged on the mounting rods.

2. The system for inactivating iridovirus in an aquaculture water body of claim 1, wherein: The upper end of the reaction kettle is in an arc shape, the top end of the reaction kettle is provided with an opening, and an exhaust device is arranged at the upper end of the opening.

3. The system for inactivating iridovirus in an aquaculture water body of claim 1, wherein: The reaction bucket is arranged at the central position in the reaction kettle through a frame body.

4. The system for inactivating iridovirus in an aquaculture water body of claim 1, wherein: The ultraviolet light emitting module adopts a 254nm wavelength ultraviolet lamp, and the ultraviolet lamp is arranged in a transverse or longitudinal direction.

5. The system for inactivating iridovirus in an aquaculture water body of claim 1, wherein: A reflective film is attached to the inner walls of the reaction kettle and the reaction bucket.

6. The system for inactivating iridovirus in an aquaculture water body of claim 1, wherein: A water outlet pipe is arranged at the lower end of the reaction bucket, the water outlet pipe extends to the outside of the reaction kettle through the side wall of the reaction kettle and the reaction kettle and is connected with other treatment equipment outside.

7. The system for inactivating iridovirus in an aquaculture body of water of claim 1, wherein: A water level sensor is further arranged in the reaction kettle, the water level sensor is arranged on the fixing frame, and the water level sensor is electrically connected with the circulating pump and the ozone generator.

8. A method of using a system for inactivating iridoviruses in an aquaculture water body according to any one of claims 1 to 7, characterized in that: The method comprises the following steps: ① The aquaculture water containing iridovirus is introduced into the pump pool, so that the water body is kept in a continuous flow state; ② The circulating pump is started to pump the water in the pump pool into the reaction kettle, and the ozone generator is started to inject ozone into the water body at the same time, the ozone concentration is controlled to be 0.3-0.5mg / L, and the ozone is uniformly dispersed in the whole water body; ③ The water body enters the water storage area in the reaction kettle from the water inlet pipe, as the water body continuously enters, after the water body fills the water storage area, the water body overflows into the reaction bucket from the top end of the reaction bucket, the ultraviolet lamps inside and outside the reaction bucket irradiate the flowing water sample at the same time, the ultraviolet light is in a UVC wave band, the irradiation wavelength is 254nm, the irradiation intensity is 20-40mW / cm², the water body enters the reaction kettle and then flows out from the water outlet pipe, and the whole operation time is controlled to be 10-20 minutes; ④ The treated water body flows out from the reaction bucket to other treatment equipment outside.

9. The method of using the system for inactivating iridoviruses in an aquaculture water body according to claim 8, characterized in that: The ultraviolet intensity is 30mW / cm², the ozone concentration is 0.3mg / L, and the ultraviolet irradiation time is 20 minutes.

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