Water decomposing agent and culture method

Abstract

The invention discloses a water decomposing agent and a culture method. The decomposing agent is prepared from the following components in percentage by mass: 30-50% of a functional microorganism composition, 45-65% of a composite carrier material and 3-8% of an auxiliary additive, wherein the functional microorganism combination comprises the following components in percentage by mass: 35%-45% of nitrifying bacteria, 25%-35% of nitrosobacteria, 15%-25% of biphase photosynthetic bacteria and 15%-25% of rhodococcus faecalis; the composite carrier material comprises the following components in percentage by mass: 50%-60% of porous ceramsite, 20%-30% of activated carbon, 10%-20% of diatomite and 1%-5% of a trace element additive. According to the compound microorganism water quality decomposing agent, through multi-strain cooperation, carrier optimization and process innovation, the comprehensive advantages of efficient degradation of pollutants, wide environmental adaptability, long-acting stability, low-cost operation and the like are achieved, and the compound microorganism water quality decomposing agent can be widely applied to the fields of sewage treatment, aquaculture, landscape water body remediation and the like and has remarkable market competitiveness and social benefits.

Description

Technical Field

[0001] This invention relates to the field of water quality decomposing agents, specifically to a water quality decomposing agent and its cultivation method. Background Technology

[0002] Urban domestic sewage is a complex type of wastewater, containing organic and inorganic matter, as well as water-soluble, sparingly soluble, or insoluble compounds. It also includes hospital wastewater and industrial wastewater. Furthermore, its flow rate is unstable and its composition fluctuates greatly. Currently, biological treatment technology is receiving widespread attention due to its advantages such as environmental friendliness and low cost.

[0003] Existing microbial water treatment agents generally suffer from the following problems: 1. They are of a single species and cannot form a complete pollutant degradation chain; 2. There are antagonistic effects between species, affecting the overall treatment; 3. They have poor environmental adaptability and their activity is significantly reduced under low temperature or extreme pH conditions; 4. They have insufficient storage stability and the number of viable bacteria decreases rapidly with storage time. Therefore, it is of great significance to develop a composite microbial water decomposition agent with efficient synergistic effects, strong environmental adaptability and good stability. Summary of the Invention

[0004] The purpose of this invention is to provide a water decomposing agent and a cultivation method to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a water quality decomposing agent, which is composed of the following components by mass percentage: 30%-50% functional microbial combination, 45%-65% composite carrier material, and 3%-8% auxiliary additives; The mass percentage ratio of each component in the functional microbial composition is as follows: nitrifying bacteria 35%-45%, nitrite-oxidizing bacteria 25%-35%, biphasic photosynthetic bacteria 15%-25%, and Rhodococcus foetida 15%-25%. The mass percentage ratio of each component in the composite carrier material is as follows: porous ceramsite 50%-60%, activated carbon 20%-30%, diatomaceous earth 10%-20%, and trace element additives 1%-5%; The mass percentage ratio of each component in the auxiliary additive is: enzyme inhibitor 2%-5%, growth factor 1%-3%.

[0006] Preferably, the present invention also includes a method for cultivating the water decomposing agent, the cultivation method specifically including the following steps: S1. Activation of bacterial strains: Freeze-dried nitrifying bacteria, nitrite-oxidizing bacteria, biphasic photosynthetic bacteria and Rhodococcus skatole were inoculated into ammonium salt medium, nitrate medium, photosynthetic bacteria medium and ISP-2 medium for culture, respectively. S2. Compound culture expansion: Mix the activated strains in a certain proportion and inoculate them into the compound culture medium; S3. Immobilization treatment: Mix the expanded bacterial culture with the composite carrier material at a ratio of 1:1.5-1:2.5, add 5-8% sodium alginate solution, and fix at 4℃ for 12 hours. S4. Drying and Packaging: Drying is carried out using a fluidized bed, followed by vacuum packaging in aluminum foil bags with added desiccant.

[0007] Preferably, the nitrifying bacteria consist of Nitrosomonas and Nitrobacterium in a live bacteria ratio of 1.5:1 to 2.5:1, and the nitrifying bacteria consist of Pseudomonas and Paracoccus denitrifying in a live bacteria ratio of 1:1 to 2:1.

[0008] Preferably, the amounts of each component added to the composite culture medium in S2 are as follows: peptone 1.2-1.8 g / L, yeast extract 0.8-1.2 g / L, glucose 0.5-1.0 g / L, brown sugar powder 1.0-2.0 g / L, white powder 2.0-3.0 g / L, bovine bone powder 0.5-1.0 g / L, NH4Cl 0.2-0.4 g / L, KNO3 0.15-0.25 g / L, KH2PO4 0.15-0.25 g / L, and MgSO4·7H2O 0.08-0.12 g / L.

[0009] Preferably, the drying temperature of the sulfurized bed in S4 is 35-40℃.

[0010] Compared with the prior art, the beneficial effects of the present invention are: 1. By scientifically proportioning nitrifying bacteria, denitrifying bacteria, photosynthetic bacteria, and Bacillus, a complete pollutant degradation chain is formed, which can achieve the degradation of organic matter, removal of ammonia nitrogen, and removal of nitrate. Under the same water quality conditions, the compound bacterial grading agent of this application can achieve a higher COD removal rate and total nitrogen removal rate compared with traditional single bacterial agents.

[0011] 2. It has strong environmental adaptability and can be used for wastewater treatment at temperatures below 10℃ and above 40℃. It has a wide pH tolerance range and strong resistance to shock loads. It can still maintain stable removal efficiency for high concentrations of pollutants.

[0012] 3. It has long-lasting stability and outstanding economic benefits: The use of porous ceramic particles and activated carbon carriers provides microecological protection, which can effectively improve the survival rate of bacteria. Sodium alginate encapsulation delays the release of bacteria, which can effectively increase the action time and at the same time reduce the amount of chemical agents used and reduce sludge production.

[0013] 4. It is fully biodegradable, produces no chemical residues, and is non-toxic to aquatic organisms; photosynthetic bacteria competitively inhibit the growth of cyanobacteria; experiments show that algal biomass can be reduced on a large scale; long-term use can increase water transparency to over 80cm and promote the reconstruction of beneficial microbial communities.

[0014] 5. Solid granules can be added directly without complicated activation steps. For wastewater treatment plant applications, they can be added directly to aerobic / anoxic tanks without facility modification. For aquaculture applications, they can be used in conjunction with aerators and biological filters for synergistic effects.

[0015] The composite microbial water decomposition agent of this invention achieves comprehensive advantages such as efficient pollutant degradation, wide environmental adaptability, long-term stability and low-cost operation through multi-strain synergy, carrier optimization and process innovation. It can be widely used in sewage treatment, aquaculture, landscape water body restoration and other fields, and has significant market competitiveness and social benefits. Detailed Implementation

[0016] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0017] This invention provides a technical solution: a water quality decomposing agent, which is composed of the following components by mass percentage: 30%-50% functional microbial combination, 45%-65% composite carrier material, and 3%-8% auxiliary additives; The functional microbial composition comprises the following components in terms of mass percentage: nitrifying bacteria 35%-45%, with nitrite-oxidizing bacteria consisting of nitrosomonas and nitrifying bacilli in a live count ratio of 1.5:1-2.5:1; nitrite-oxidizing bacteria 25%-35%, with pseudomonas and denitrifying paracocci in a live count ratio of 1:1-2:1; biphasic photosynthetic bacteria 15%-25%; and Rhodococcus foetida 15%-25%. The mass percentage ratio of each component in the composite carrier material is as follows: porous ceramsite 50%-60%, activated carbon 20%-30%, diatomaceous earth 10%-20%, and trace element additives 1%-5%; The mass percentage ratio of each component in the auxiliary additive is as follows: 2%-5% of the enzyme inhibitor, in which protease, lipase and cellulase are composed in an activity unit ratio of 1:0.8:1.2; 1%-3% of the growth factors, which mainly include B vitamins and biotin.

[0018] Specifically, the present invention also includes a method for cultivating the water decomposing agent, the cultivation method specifically including the following steps: S1. Activation of bacterial strains: Freeze-dried nitrifying bacteria, nitrite-oxidizing bacteria, biphasic photosynthetic bacteria and Rhodococcus skatole were inoculated into ammonium salt medium, nitrate medium, photosynthetic bacteria medium and ISP-2 medium respectively for culture. The culture temperature was controlled at 20-30℃ and the culture time was 24-48 hours. S2. Compound culture expansion: Mix the activated strains in a certain proportion and inoculate them into the compound culture medium; S3. Immobilization treatment: Mix the expanded bacterial culture with the composite carrier material at a ratio of 1:1.5-1:2.5, add 5-8% sodium alginate solution, and fix at 4℃ for 12 hours. S4. Drying and Packaging: Drying is carried out using a fluidized bed at a temperature controlled at 35-40℃, followed by vacuum packaging in aluminum foil bags with added desiccant.

[0019] Specifically, the amounts of each component added to the S2 composite culture medium are as follows: peptone 1.2-1.8 g / L, yeast extract 0.8-1.2 g / L, glucose 0.5-1.0 g / L, brown sugar powder 1.0-2.0 g / L, white powder 2.0-3.0 g / L, bovine bone meal 0.5-1.0 g / L, NH4Cl 0.2-0.4 g / L, KNO3 0.15-0.25 g / L, KH2PO4 0.15-0.25 g / L, and MgSO4·7H2O 0.08-0.12 g / L. The culture temperature is controlled at 28-30℃, the culture time is 36-48 hours, the dissolved oxygen is controlled at ≥4 mg / L, and the pH is 7.2-7.8.

[0020] 1. In the experiment verifying the synergistic effect of bacterial strains: Simulated wastewater: COD 300 mg / L, NH4 + 20 mg / L, NO2 - The experimental subjects were: a single bacterial strain and the water decomposing agent of this invention, with a concentration of 5 mg / L, a temperature of 25℃, a pH of 7.5, and a DO concentration of ≥4 mg / L. After 24 hours of processing, the experimental results are as follows:

[0021] It can be concluded that the water decomposing agent of the present invention achieves a significantly higher overall pollutant removal rate than a single bacterial strain through synergistic effects.

[0022] 2. In the temperature adaptability test experiment: The water decomposing agent of the present invention was added to a water body with the same pollution load (COD 200 mg / L, NH4) + 15 mg / L), testing NH4 at different temperatures for 72 hours. + Removal rate, the following are experimental data:

[0023] It can be concluded that it maintains high activity within the range of 10-40℃, and is especially suitable for areas with large temperature differences.

[0024] 3. Experimental data from an actual wastewater treatment plant: In a municipal wastewater treatment capacity of 100 tons / day, the influent water quality is COD 350 mg / L and NH4+. + 40 mg / L, TN 60 mg / L, the dosage of the water decomposing agent of this invention is 50 g / ton of wastewater, and it is operated continuously for 30 days. The following are the experimental data:

[0025] It can be concluded that, compared with the traditional activated sludge process, the water decomposition agent of this invention improves the COD removal rate by 22% and reduces energy consumption by 15%.

[0026] 4. In practical applications, experiments have shown that when the initial concentration of NH4 is... + 2.8 mg / L, NO2 - In an aquaculture pond with a concentration of 2.1 mg / L, the water quality decomposing agent of this invention was added once a week at a dosage of 10 g / m³. The concentration was then tested again after 7 days. NH4 content was... + NO2 decreased to 0.6 mg / L. - It decreased to 0.3 mg / L.

[0027] This invention forms a complete pollutant degradation chain by scientifically proportioning nitrifying bacteria, denitrifying bacteria, photosynthetic bacteria, and Bacillus, which can achieve the degradation of organic matter, removal of ammonia nitrogen, and removal of nitrate. Under the same water quality conditions, the compound bacterial grading agent of this application can achieve a higher COD removal rate and total nitrogen removal rate compared with traditional single bacterial agents.

[0028] Meanwhile, the water decomposing agent of this invention has strong environmental adaptability, suitable for wastewater treatment below 10℃ and above 40℃, with a high pH tolerance range and strong resistance to shock loads, and can maintain stable removal efficiency even for high concentrations of pollutants; the use of porous ceramsite and activated carbon carriers provides micro-ecological protection, which can effectively improve the survival rate of bacteria, and sodium alginate encapsulation delays the release of bacteria, which can effectively increase the action time and at the same time reduce the amount of chemical agents used and reduce sludge production; it is fully biodegradable, will not produce chemical residues, and is non-toxic to aquatic organisms; photosynthetic bacteria competitively inhibit the growth of cyanobacteria; experiments show that algal biomass can be reduced on a large scale; long-term use can increase water transparency to more than 80cm and promote the reconstruction of beneficial microbial communities; the solid granules can be directly added without complicated activation steps, and for wastewater treatment plant applications, it can be directly added to aerobic / anoxic tanks without the need for facility modification; for aquaculture, it can be used in conjunction with aerators and biological filters for synergistic effect.

[0029] Therefore, the composite microbial water decomposition agent of the present invention achieves comprehensive advantages such as efficient degradation of pollutants, wide environmental adaptability, long-term stability and low-cost operation through multi-species synergy, carrier optimization and process innovation. It can be widely used in sewage treatment, aquaculture, landscape water body restoration and other fields, and has significant market competitiveness and social benefits.

[0030] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A water-decomposing agent, characterized in that: The decomposing agent is composed of the following components by weight percentage: 30%-50% functional microbial combination, 45%-65% composite carrier material, and 3%-8% auxiliary additives; The mass percentage ratio of each component in the functional microbial composition is as follows: nitrifying bacteria 35%-45%, nitrite-oxidizing bacteria 25%-35%, biphasic photosynthetic bacteria 15%-25%, and Rhodococcus foetida 15%-25%. The mass percentage ratio of each component in the composite carrier material is as follows: porous ceramsite 50%-60%, activated carbon 20%-30%, diatomaceous earth 10%-20%, and trace element additives 1%-5%; The mass percentage ratio of each component in the auxiliary additive is: enzyme inhibitor 2%-5%, growth factor 1%-3%.

2. The water decomposing agent and cultivation method according to claim 1, characterized in that: Specifically, the following steps are included: S1. Activation of bacterial strains: Freeze-dried nitrifying bacteria, nitrite-oxidizing bacteria, biphasic photosynthetic bacteria and Rhodococcus skatole were inoculated into ammonium salt medium, nitrate medium, photosynthetic bacteria medium and ISP-2 medium for culture, respectively. S2. Compound culture expansion: Mix the activated strains in a certain proportion and inoculate them into the compound culture medium; S3. Immobilization treatment: Mix the expanded bacterial culture with the composite carrier material at a ratio of 1:1.5-1:2.5, add 5-8% sodium alginate solution, and fix at 4℃ for 12 hours. S4. Drying and Packaging: Drying is carried out using a fluidized bed, followed by vacuum packaging in aluminum foil bags with added desiccant.

3. The water decomposing agent according to claim 1, characterized in that: The nitrifying bacteria consist of Nitrosomonas and Nitrobacterium at a live count ratio of 1.5:1 to 2.5:1, and the nitrifying bacteria consist of Pseudomonas and Paracoccus denitrifying at a live count ratio of 1:1 to 2:

1.

4. The water decomposing agent and cultivation method according to claim 2, characterized in that: The amounts of each component added to the composite culture medium in S2 are as follows: peptone 1.2-1.8 g / L, yeast extract 0.8-1.2 g / L, glucose 0.5-1.0 g / L, brown sugar powder 1.0-2.0 g / L, white powder 2.0-3.0 g / L, bovine bone powder 0.5-1.0 g / L, NH4Cl 0.2-0.4 g / L, KNO3 0.15-0.25 g / L, KH2PO4 0.15-0.25 g / L, and MgSO4·7H2O 0.08-0.12 g / L.

5. The water decomposing agent and cultivation method according to claim 2, characterized in that: The drying temperature of the sulfurized bed in S4 is 35-40℃.

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