Cuttage breeding method of seashore cattail wood

Abstract

The invention provides a cottage breeding method for seashore cattail wood, and relates to the technical field of plant cottage seedling raising. According to the cuttage breeding method of the beach clerodendranthus spicatus, NAA, IAA, tocopherol acetate, ethyl alcohol and a laguguan leaf extract are adopted as rooting agents to treat branches of the beach clerodendranthus spicatus, and then cuttage management is conducted, wherein the laguguan leaf extract is obtained by freeze-drying laguguan leaves, smashing the laguguan leaves, conducting acid treatment on the laguguan leaves and then conducting ethanol extraction on the laguguan leaves. According to the method, the defects in the prior art are overcome, the seedling raising period of the seashore clerodendranthus spicatus is effectively shortened in a cutting seedling raising mode, meanwhile, rooting and survival of cutting seedlings are further promoted, and the seedling raising efficiency is comprehensively improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of plant cutting seedling raising, in particular to a cutting breeding method of Dolichan drone spathacea. BACKGROUND

[0002] Dolichan drone spathacea is a evergreen tree of Planchonella in Lecythidaceae, which is distributed in Hainan and Guangdong provinces, and has been listed in the list of key protected wild plants in Hainan Province. At present, the breeding method of Dolichan drone spathacea is mainly seed breeding. The seed breeding Dolichan drone spathacea has a 6-month seedling rate of about 82.22%, and under careful management, the highest growth is 40cm in 4 months, the maximum base diameter is 7mm, the overall seedling period is long, the seedling management cost is high, and the investment in seedling environment and pest control is large.

[0003] Cutting seedling raising is a common asexual propagation method of plants, which cuts the stems, leaves, roots and other nutrient organs of plants to cultivate independent new plants. Its core advantages lie in high propagation efficiency, strong genetic stability, simple operation, low cost and the like. At present, there is no related report on cutting seedling raising of Dolichan drone spathacea, but based on the advantages of existing plant cutting seedling raising, it is crucial to design a cutting seedling raising method of Dolichan drone spathacea to shorten the seedling period of Dolichan drone spathacea and realize rapid batch production of endangered plant Dolichan drone spathacea seedlings. SUMMARY

[0004] In view of the deficiencies in the prior art, the present application provides a cutting breeding method of Dolichan drone spathacea, which effectively shortens the seedling period of Dolichan drone spathacea by cutting seedling raising, and further promotes the rooting and survival of cutting seedlings, and comprehensively improves the seedling efficiency.

[0005] To achieve the above purpose, the present application is realized by the following technical scheme: A cutting breeding method of Dolichan drone spathacea, the cutting breeding method comprising the following steps: S1, preparation of cutting branches: selecting 1-2 year old Dolichan drone spathacea, cutting branches with a diameter of 0.5-2.5cm and a length of 25-30cm, cutting the upper end flat, cutting the lower end obliquely, and then disinfecting to obtain cutting branches for standby; S2, rooting agent soaking treatment: the above cuttings are placed at 10°C for 2-3 h, then the lower end 3-8 cm is soaked in normal temperature rooting agent for 30-50 min to obtain pretreated cuttings; and the rooting agent is prepared by the following formula: NAA 200-300 mg / L+IAA 200-300 mg / L+tocopherol acetate 40-80 mg / L+ethanol 10-20 mL / L+Rauvolfia verticillata leaf extract 15-30 mg / L+water to 1 L; the Rauvolfia verticillata leaf extract is obtained by freeze-drying, crushing, acid treatment and ethanol extraction of Rauvolfia verticillata leaves; S3, cutting treatment: the lower end of the above pretreated cutting is cut into the sterilized cutting medium, and the cutting medium is poured with clear water for daily management.

[0006] Preferably, the cutting in step S1 retains 0-3 leaves, and when the cutting is a top-containing branch, the upper end of the branch is retained.

[0007] Preferably, the sterilization method in step S1 is to soak the branch base 2-5 cm in a 0.1%-0.2% potassium permanganate solution for 10-12 h, and then wash the base with water to remove residual liquid.

[0008] Preferably, the preparation method of the Rauvolfia verticillata leaf extract comprises the following steps: S2-1, freeze-drying the fresh Rauvolfia verticillata leaves, crushing to obtain Rauvolfia verticillata leaf powder for use; S2-2, ultrasonic treatment of the Rauvolfia verticillata leaf powder in an acetic acid solution with a pH of 4-4.5 for 20-30 min, then filtering, removing the filtrate, washing the filter residue with water to neutral, obtaining acid-treated material for use; S2-3, adding the above acid-treated material to an 85% ethanol solution, ultrasonic extraction at 45-50°C for 15-20 min, filtering, adding the filter residue to the 85% ethanol solution again and repeating the operation 2-3 times, and freeze-drying the combined filtrate to obtain the Rauvolfia verticillata leaf extract.

[0009] Preferably, the preparation method of the rooting agent in step S2 is to disperse tocopherol acetate and Rauvolfia verticillata leaf extract into ethanol, then mix water, NAA, IAA and stir uniformly to prepare.

[0010] Preferably, the cutting medium in step S3 is a mixture of sand, red soil and organic fertilizer.

[0011] Preferably, the sterilization method of the cutting medium in step S3 is to pour the cutting medium with a 0.1%-0.5% potassium permanganate solution, then cover the film and seal for 2-4 h, then uncover the film and air dry for 2-3 d.

[0012] Preferably, the depth of the pretreated cutting in step S3 is 8-10 cm.

[0013] Preferably, the step S3 daily management is in the way of keeping the cutting substrate humidity between 30%-90%, temperature at 20℃-30℃, and light shielding rate at 20%-80%.

[0014] The present application provides a cutting breeding method of beach cat tail wood, which has the advantages of the prior art. By treating the cutting branches of beach cat tail wood with plant growth hormone compound tocopheryl acetate, laguncularia leaves extract and other components, the growth of the root of the cutting seedling is effectively promoted. Meanwhile, the branches are treated at low temperature before soaking in the rooting agent, which further improves the activity of the cutting branches, promotes early rooting of the branches, and comprehensively improves the survival rate and rooting effect of the beach cat tail wood cutting seedlings, and shortens the seedling raising cycle of the beach cat tail wood. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 The beach cat tail wood branches cut for the present application; Figure 2 The schematic diagram of the cutting branches of each control group and experimental group in the embodiment of the present application soaked in the rooting agent; Figure 3 The growth schematic diagram of the cutting branches of control group 1 in the embodiment of the present application after cutting for 30d, wherein the left side is the whole plant growth schematic diagram, and the right side is the enlarged schematic diagram of the root growth; Figure 4 The growth schematic diagram of the cutting branches of control group 2 in the embodiment of the present application after cutting for 30d, wherein the left side is the whole plant growth schematic diagram, and the right side is the enlarged schematic diagram of the root growth; Figure 5 The growth schematic diagram of the cutting branches of experimental group 1 in the embodiment of the present application after cutting for 30d, wherein the left side is the whole plant growth schematic diagram, and the right side is the enlarged schematic diagram of the root growth; Figure 6 The growth schematic diagram of the cutting branches of experimental group 2 in the embodiment of the present application after cutting for 30d, wherein the left side is the whole plant growth schematic diagram, and the right side is the enlarged schematic diagram of the root growth; Figure 7 The growth schematic diagram of the cutting branches of experimental group 3 in the embodiment of the present application after cutting for 30d, wherein the left side is the whole plant growth schematic diagram, and the right side is the enlarged schematic diagram of the root growth; Figure 8 The growth schematic diagram of the cutting branches of experimental group 4 in the embodiment of the present application after cutting for 30d, wherein the left side is the whole plant growth schematic diagram, and the right side is the enlarged schematic diagram of the root growth; Figure 9 The growth schematic diagram of the cutting branches of experimental group 5 in the embodiment of the present application after cutting for 30d, wherein the left side is the whole plant growth schematic diagram, and the right side is the enlarged schematic diagram of the root growth; Figure 10 Figure 1 is a growth diagram of the cuttings of the experimental group 6 in the embodiment of the present application for 30 days, in which the left side is a diagram of the whole plant growth, and the right side is an enlarged diagram of the root growth. DETAILED DESCRIPTION

[0016] In order to make the purpose, technical scheme and advantages of the embodiment of the present application more clear, the technical scheme in the embodiment of the present application is described clearly and completely below in combination with the embodiment of the present application. Obviously, the described embodiment is a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiment in the present application, all the other embodiments obtained by the person skilled in the art without creative labor are within the protection scope of the present application.

[0017] Preparation of the extract of the leaves of Laggera alata 1. Preparation of LGM-A (1) Freeze-dry the fresh leaves of Laggera alata, crush them, and obtain the leaf powder of Laggera alata for use; (2) Put the leaf powder of Laggera alata into an acetic acid solution with a pH of 4, and treat it with ultrasonic waves at 600 W for 20 min. Then, filter the solution, remove the filtrate, and wash the filter residue with clean water until it is neutral. Thus, the acid-treated material is obtained for use; (3) Put the acid-treated material into 10 times the volume of an 85% ethanol solution, and treat it with ultrasonic waves at 400 W for 15 min at a temperature of 45°C. Then, filter the solution, repeat the above-mentioned ultrasonic extraction step with ethanol three times, and freeze-dry the combined filtrate. Thus, the extract of the leaves of Laggera alata is obtained, which is denoted as LGM-A.

[0018] 2. Preparation of LGM-B (1) Freeze-dry the fresh leaves of Laggera alata, crush them, and obtain the leaf powder of Laggera alata for use; (2) Put the leaf powder of Laggera alata into 10 times the volume of an 85% ethanol solution, and treat it with ultrasonic waves at 400 W for 15 min at a temperature of 45°C. Then, filter the solution, repeat the above-mentioned ultrasonic extraction step with ethanol three times, and freeze-dry the combined filtrate. Thus, the extract of the leaves of Laggera alata is obtained, which is denoted as LGM-B.

[0019] Preparation of the cutting substrate Mix sand, red soil and organic fertilizer in equal amounts, pour a potassium permanganate solution with a mass concentration of 0.5% into the mixture, mix well, cover with plastic wrap, seal and fumigate for 3 h, then uncover the plastic wrap and air dry for 2 d. Thus, the cutting substrate is obtained.

[0020] Embodiment 1 Cutting propagation of the beach cat tail wood (1) Select 1-2 years old sea cat tail wood, cut the diameter of 0.5-2.5 cm, length of 25-30 cm branch (reserve 0-3 leaf, and when the cutting branch is top containing branch, reserve the upper end of the tender branch), the upper end of the branch is cut, the lower end is cut, the lower end is cut, and the lower end is cut. 2-5 cm of the branch base is soaked in 0.2% potassium permanganate solution for 10h, then washed with water to remove excess medicine, and the disinfection is completed, and the cutting branch is prepared for use; (2) The above cutting branch is placed at 10℃ for 3h, and then the lower end 3-8cm is immersed in the rooting agent at room temperature for 40min, and the pretreated cutting branch is obtained; (3) The lower end 8-10cm of the above pretreated cutting branch is inserted into the disinfected cutting substrate, and the cutting substrate is poured with water, the humidity of the cutting substrate is controlled at 30%-90%, the temperature range of the whole cutting is 20℃-30℃, the average temperature is about 24℃, and the light shielding rate is set at 50%. The conventional management is observed, and the cutting rooting condition is observed.

[0021] Comparative example 1: Cutting propagation of sea cat tail wood: The specific cutting propagation method is basically the same as that of example 1, only the step (2) is different: (2) The lower end 3-8cm of the above cutting branch is immersed in the rooting agent at room temperature for 40min, and the pretreated cutting branch is obtained; Experimental detection: I. Preparation of rooting agent: Different rooting agents are prepared according to the following method: 1. Preparation of rooting agent 1: 60mg of tocopherol acetate, 20mg of LGM-A is dissolved in 15mL of ethanol, fully stirred and mixed with 250mg of NAA and 250mg of IAA, and water is added to 1L to obtain rooting agent 1.

[0022] 2. Preparation of rooting agent 2: 60mg of tocopherol acetate, 20mg of LGM-B is dissolved in 15mL of ethanol, fully stirred and mixed with 250mg of NAA and 250mg of IAA, and water is added to 1L to obtain rooting agent 2; 3. Preparation of rooting agent 3: 20mg of LGM-A is dissolved in 15mL of ethanol, fully stirred and mixed with 250mg of NAA and 250mg of IAA, and water is added to 1L to obtain rooting agent 3.

[0023] 4. Preparation of rooting agent 4: Dissolve 60 mg of tocopheryl acetate in 15 mL of ethanol, stir thoroughly, then mix with 250 mg of NAA and 250 mg of IAA, add water to make up to 1 L, to obtain rooting agent 4.

[0024] 5. Preparation of rooting agent 5: Mix 250mg of NAA and 250mg of IAA, add water to make up to 1L, and you will get rooting agent 5.

[0025] II. Rooting Experiment of Cuttings: Referring to Table 1 below, different cutting propagation methods and rooting agents were selected to set up different cutting experimental groups and control groups (the soaking of cuttings in rooting agent for each group is as follows). Figure 2 (as shown) Table 1 III. Experimental Statistics: 1. The rooting rate (rooting rate = rooted branches / total cuttings) and survival rate (survival rate = surviving branches / total cuttings) of each group of *Cervifolia spp.* cuttings were statistically analyzed at 15 days and 30 days after cutting. The specific results are shown in Table 2 below: Table 2 As shown in the table above, when water is used as a rooting agent, treating the cuttings according to the method in Example 1, that is, treating the cuttings at low temperature before planting (control group 1), can improve the early rooting rate to a certain extent compared with planting without low temperature treatment (control group 2), but the overall survival rate of the cuttings is reduced. In addition, the rooting agent with acid-treated LGM-A (experimental group 1) showed a significantly higher rooting rate at 15 days compared to the one without acid-treated LGM-B (experimental group 2), and the overall survival rate was also higher. However, the addition of LGM-B without acid treatment to the rooting agent could lead to a certain degree of reduction in the early survival rate of the cuttings, affecting the overall cutting effect. Compared with the experimental group 3, which did not add tocopherol acetate, the experimental group 1 with added rooting agent could effectively improve the early rooting rate and survival rate, thus ensuring the overall efficiency of cutting propagation and seedling cultivation.

[0026] 2. Thirty days after cutting, five rooted cuttings were taken from each group, and the average number of roots (main roots) and average root length were calculated. The specific results are shown in Table 3 below, and the rooting status of each group is as follows. Figures 3-10 As shown: Table 3 From table 3 above and Figures 3-10It can be seen that the rooting number of the experimental group 2 is relatively more, but the average root length is significantly shortened, that is, the use of LGM-B may inhibit the elongation of the cutting root, and to a certain extent, affect the quality of the cutting seedling. The experimental group 1 using LGM-A and tocopherol acetate can significantly ensure the rooting number and root length, and comprehensively improve the quality of the cutting seedling.

[0027] The above examples are only used to illustrate the technical solutions of the present application, but not to limit it; although the present application has been described in detail with reference to the foregoing examples, those skilled in the art should understand that the technical solutions recorded in the foregoing examples can be modified, or some technical features can be replaced by equivalents; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A method for the vegetative propagation of Chionanthus retusus, characterized in that, The cutting breeding method comprises the following steps: S1, cutting branch preparation: selecting 1-2 year old sea cat tail wood, cutting the branches with a diameter of 0.5-2.5 cm and a length of 25-30 cm, cutting the upper end flat and the lower end obliquely, then disinfecting to obtain cutting branches for standby; S2, rooting agent soaking treatment: placing the above cutting branches at 10°C for 2-4 h, then immersing the lower end 3-8 cm in the rooting agent at room temperature for 30-50 min to obtain pretreated cutting branches; and the rooting agent has a formula of NAA 200-300 mg / L+IAA 200-300 mg / L+tocopherol acetate 40-80 mg / L+ethanol 10-20 mL / L+rauwohia fabric extract 15-30 mg / L+water to 1 L; the rauwohia fabric extract is obtained by freeze-drying, crushing, acid treatment and ethanol extraction of rauwohia leaves; S3, cutting treatment: cutting the lower end of the above pretreated cutting branches into the disinfected cutting substrate, pouring clean water, and daily management.

2. The method of claim 1, wherein the cuttage of the sea grape is characterized by: In the step S1, 0-3 leaf blades of the cutting branch are reserved, and when the cutting branch is a top-containing branch, the upper end of the tender branch leaf is reserved.

3. The method of claim 1, wherein the cuttage of the sea grape is characterized by: In the step S1, the disinfection method is to immerse the branch base 2-5 cm in a potassium permanganate solution with a concentration of 0.1%-0.2% for 10-12 h, and then wash the base with water to remove residual liquid medicine.

4. The method of claim 1, wherein the cuttage of the sea grape is performed by cutting the sea grape in the spring season. The preparation method of the rauwohia fabric extract comprises the following steps: S2-1, freeze-drying the fresh rauwohia leaves, crushing to obtain rauwohia leaf powder for standby; S2-2, ultrasonic treatment of the rauwohia leaf powder in an acetic acid solution with a pH of 4-4.5 for 20-30 min, then filtering, removing the filtrate, washing the filter residue with water to neutral, and obtaining the acid-treated material for standby; S2-3, adding the above acid-treated material into an 85% ethanol solution, ultrasonic extraction at 45-50°C for 15-20 min, filtering, adding the filter residue into the 85% ethanol solution again and repeating the operation 2-3 times, and freeze-drying the combined filtrate to obtain the rauwohia fabric extract.

5. The method of claim 1, wherein the cuttage of the sea grape is characterized by: The preparation method of the rooting agent in the step S2 is to disperse the tocopherol acetate and the rauwohia fabric extract into ethanol, then mix water, NAA, IAA, and stir uniformly to obtain.

6. The method of claim 1, wherein the cuttage of the sea grape is characterized by: The cutting substrate in the step S3 is a mixture of sand, red soil, and organic fertilizer.

7. The method of claim 1, wherein the cuttage of the sea grape is characterized by: The disinfection method of the cutting substrate in the step S3 is to pour the cutting substrate with a potassium permanganate solution with a concentration of 0.1%-0.5% and then seal the film for 2-4 h, and then uncover the film and air dry for 2-3 d.

8. The method of claim 1, wherein the cuttage of the sea grape is characterized by: The depth of the cutting of the pretreated cutting branch in the step S3 is 8-10 cm.

9. The method of claim 1, wherein the cuttage of the sea grape is characterized by: The daily management method in the step S3 is to keep the humidity of the cutting substrate between 30%-90%, the temperature at 20-30°C, and the light shielding rate at 20%-80%.

Images