Capsule for relieving enteritis as well as preparation method and application thereof

Abstract

The invention discloses a capsule for relieving enteritis as well as a preparation method and application thereof. The capsule is prepared from the following components in parts by weight: camellia oil, salvia miltiorrhiza, fructus forsythiae, scutellaria baicalensis, dandelion, honeysuckle, emblic leafflower fruit, poria cocos, corydalis tuber, dendrobe and rehmannia. The molecular mechanism of the camellia oil for relieving inflammation is explored by utilizing research means such as a network pharmacology technology, a molecular docking technology and metabonomics, the fact that the camellia oil can inhibit the secretion of inflammatory factors from multiple components, multiple targets and multiple ways is found, and the anti-inflammatory pharmacological action of the camellia oil is systematically explored; from the perspective of intestinal inflammation, the inflammation inhibition mechanism of the camellia oil is researched, and the camellia oil can clarify the anti-inflammatory action mechanism of the camellia oil from two aspects of regulating protein and substance metabolism by relieving intestinal inflammation, repairing intestinal injury, reducing intestinal endocrine cell apoptosis and recovering food inhibition intestinal hormone secretion. Key nutritional ingredients in the camellia oil are screened out according to research results, and the key nutritional ingredients are proportioned, optimized, designed and produced into related products.

Description

Technical Field

[0001] This invention relates to the field of woody compound traditional Chinese medicine, specifically to a capsule for relieving enteritis, its preparation method, and its application. Background Technology

[0002] Camellia oil is a unique woody plant oil native to my country, rich in nutrients and possessing high medicinal value. It is a major raw material source for the food and pharmaceutical industries. Studies have shown that camellia oil contains various beneficial nutrients such as squalene and phytosterols. Developing products based on camellia oil suitable for people with inflammatory bowel disease provides a foundation for therapeutic food products.

[0004] Current status of research at home and abroad:

[0005] Camellia oil, due to its significant benefits in improving intestinal function and preventing gastrointestinal ulcers, has attracted considerable research and development from various medical institutions in China. More and more researchers are beginning to explore the therapeutic effects of camellia oil extracts on inflammation. Studies have shown that adding 33-200 μg / mL of camellia oil to an inflamed RAW264.7 macrophage model significantly inhibits nitric oxide (NO) secretion stimulated by lipopolysaccharide (LPS), thereby improving the inflammatory response. The mechanism is that the polyphenols in camellia oil inhibit the expression of Nos2 and Cox2 genes, thus achieving an anti-inflammatory effect. Recently, clinical efficacy of combined ginger and camellia oil in treating pressure ulcers has also been reported in China. The results show that the combined application of ginger and camellia oil significantly improves the treatment of pressure ulcers, indicating that the anti-inflammatory and anti-inflammatory effects of camellia oil are gradually gaining clinical recognition.

[0006] The research results indicate that camellia oil, rich in numerous nutrients, has a good protective effect against inflammation. However, further research is needed to determine which of these active ingredients are the main factors. The optimal content ratio between substances (such as linolenic acid:linoleic acid) is also one of the factors influencing inflammation. These aspects require reasonable preparation processes and extraction methods and are aspects that need to be considered in practical applications. As a common and frequently occurring disease, gastroenteritis has a long course and is prone to recurrence. Although there are numerous drugs and methods for treating inflammation, many drugs have significant side effects, are not suitable for long-term use, and are prone to drug resistance. Therefore, establishing high-value-added products based on camellia oil will greatly promote the upgrading of the entire industry, and the therapeutic effect of camellia oil extract on inflammation will be one of the future research directions. Summary of the Invention

[0007] To address the technical problems existing in the background art, the present invention provides a capsule for relieving enteritis, its preparation method and application, which has the effect of relieving enteritis, can provide guidance for the optimization of traditional Chinese medicine compound prescriptions, and provide new treatment ideas for relieving intestinal inflammation and other related diseases.

[0008] A capsule for relieving enteritis is made from the following components in parts by weight: 1-100 parts camellia oil, 1-100 parts salvia miltiorrhiza, 1-100 parts forsythia suspensa, 1-100 parts scutellaria baicalensis, 1-100 parts dandelion, 1-100 parts honeysuckle, 1-100 parts amla, 1-100 parts poria cocos, 1-100 parts corydalis yanhusuo, 1-100 parts dendrobium, and 1-100 parts rehmannia glutinosa.

[0009] Preferably, it is made from the following components in parts by weight: 1-100 parts camellia oil, 51-100 parts salvia miltiorrhiza, 51-100 parts forsythia suspensa, 51-100 parts scutellaria baicalensis, 51-100 parts dandelion, 51-100 parts honeysuckle, 1-50 parts amla, 1-50 parts poria cocos, 1-50 parts corydalis yanhusuo, 1-50 parts dendrobium, and 1-50 parts rehmannia glutinosa.

[0010] Preferably, it is made from the following components in parts by weight: 1-100 parts camellia oil, 1-50 parts salvia miltiorrhiza, 1-50 parts forsythia suspensa, 1-50 parts scutellaria baicalensis, 1-50 parts dandelion, 1-50 parts honeysuckle, 51-100 parts amla, 51-100 parts poria cocos, 51-100 parts corydalis yanhusuo, 51-100 parts dendrobium, and 51-100 parts rehmannia glutinosa.

[0011] Preferably, it is made from the following components in parts by weight: 1-100 parts camellia oil, 10-90 parts salvia miltiorrhiza, 10-90 parts forsythia suspensa, 10-90 parts scutellaria baicalensis, 10-90 parts dandelion, 10-90 parts honeysuckle, 10-90 parts amla, 10-90 parts poria cocos, 10-90 parts corydalis yanhusuo, 10-90 parts dendrobium, and 10-90 parts rehmannia glutinosa.

[0012] Preferably, it is made from the following components in parts by weight: 1-100 parts camellia oil, 20-80 parts salvia miltiorrhiza, 20-80 parts forsythia suspensa, 20-80 parts scutellaria baicalensis, 20-80 parts dandelion, 20-80 parts honeysuckle, 20-80 parts amla, 20-80 parts poria cocos, 20-80 parts corydalis yanhusuo, 20-80 parts dendrobium, and 20-80 parts rehmannia glutinosa.

[0013] A capsule for relieving enteritis, the preparation method of which includes the following steps:

[0014] (1) Compound preparation: Select Salvia miltiorrhiza, Forsythia suspensa, Scutellaria baicalensis, Taraxacum mongolicum, Lonicera japonica, Phyllanthus emblica, Poria cocos, Corydalis yanhusuo, Dendrobium nobile, Rehmannia glutinosa and Camellia chinensis oil for compound preparation;

[0015] (2) Camellia oil component analysis: By using analytical tools, the therapeutic targets of effective components of camellia oil can be comprehensively predicted by utilizing the principles of chemical structure similarity and active functional group pairing. A relevant "camellia oil effective components-anti-inflammatory targets" network and corresponding protein interaction network are constructed.

[0016] (3) By using optimization tools to provide optimization technology for traditional Chinese medicine compound prescriptions, the optimal compound medicinal materials and their proportions are obtained, and a pilot product of camellia oil traditional Chinese medicine compound capsules is formed;

[0017] (4) Verification: Verify the exact effect of camellia oil on rat intestinal inflammation. Using LPS-induced inflammatory cells and animals as models, and with the help of evaluation parameters, conduct research on the anti-inflammatory function of camellia oil. Analyze the concentration of the inducer, relative cell viability and key enzymes of inflammatory metabolism, and further study the mechanism by which camellia oil regulates intestinal inflammation, so as to provide support and basis for improving the added value of camellia oil.

[0018] (5) Capsule making, encapsulation, and packaging.

[0019] In a preferred preparation method, multi-dose metabolomics analysis is performed on the anti-inflammatory cells and animal experimental models of camellia oil to analyze the anti-inflammatory mechanism of camellia oil from the aspects of inflammatory factor metabolism and lipid metabolism.

[0020] In a preferred preparation method, the tools used in step (2) are SwissTargetPrediction, SEA, Pharmapper, and Superpre.

[0021] In a preferred preparation method, the optimization tool for step (3) is a KEGG and GO analysis optimization tool.

[0022] An application for capsules to relieve enteritis involves combining the anti-inflammatory active ingredients of camellia oil with traditional Chinese medicine ingredients in the preparation of products to relieve human enteritis.

[0023] (1) This study explored the molecular mechanism by which camellia oil relieves inflammation using network pharmacology, molecular docking and metabolomics. It found that camellia oil may inhibit the secretion of inflammatory factors through multiple components, multiple targets and multiple pathways. The pharmacological effects of camellia oil on anti-inflammation were systematically explored.

[0024] (2) This study investigates the mechanism by which camellia oil inhibits inflammation from the perspective of intestinal inflammation. It suggests that camellia oil can alleviate intestinal inflammation, repair intestinal damage, reduce apoptosis of intestinal endocrine cells, and restore the secretion of appetite-suppressing intestinal hormones. The anti-inflammatory mechanism of camellia oil is elucidated from the perspectives of regulating protein and metabolism. Based on the research results, key nutrients in camellia oil were screened and their proportions were optimized for the design and production of related products.

[0025] The usage of the camellia oil capsules of the present invention, which have the function of relieving enteritis, is as follows: oral administration, two or three times a day, once in the morning and once in the evening, or once in the morning, noon and once in the evening, one capsule each time, preferably taken 0.5-1 hour before meals, or taken directly or with warm water.

[0026] Beneficial effects:

[0027] 1. A camellia oil compound traditional Chinese medicine capsule formula with the effect of relieving enteritis was obtained through KEGG and GO analysis and optimization;

[0028] 2. Prepare camellia oil with high anti-inflammatory efficacy. The product contains more than 8 kinds of fatty acids and the content of primary oxidation products (PV, AV, K232) is reduced. The test results show that the anti-inflammatory ability is increased by more than 20% compared with the process before improvement.

[0029] 3. Completed a functional evaluation study on camellia oil's ability to alleviate intestinal inflammation in rats. By inhibiting the NF-κB signaling pathway and reducing the levels of inflammatory factors TNF-α and IL-1β, camellia oil improved the intestinal inflammatory state in rats, providing a basis for the safe use and anti-enteritis effects of camellia oil. Detailed Implementation

[0030] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0031] Example 1: A capsule for relieving enteritis, made from the following components in parts by weight: 15 parts camellia oil, 15 parts salvia miltiorrhiza, 15 parts forsythia suspensa, 15 parts scutellaria baicalensis, 15 parts dandelion, 15 parts honeysuckle, 15 parts amla, 15 parts poria cocos, 15 parts corydalis yanhusuo, 15 parts dendrobium, and 15 parts rehmannia glutinosa.

[0032] A capsule for relieving enteritis, the preparation method of which includes the following steps:

[0033] (1) Compound preparation: Select Salvia miltiorrhiza, Forsythia suspensa, Scutellaria baicalensis, Taraxacum mongolicum, Lonicera japonica, Phyllanthus emblica, Poria cocos, Corydalis yanhusuo, Dendrobium nobile, Rehmannia glutinosa and Camellia chinensis oil for compound preparation;

[0034] (2) Camellia oil component analysis: By using analytical tools, the therapeutic targets of effective components of camellia oil can be comprehensively predicted by utilizing the principles of chemical structure similarity and active functional group pairing. A relevant "camellia oil effective components-anti-inflammatory targets" network and corresponding protein interaction network are constructed.

[0035] (3) By using optimization tools to provide optimization technology for traditional Chinese medicine compound prescriptions, the optimal compound medicinal materials and their proportions are obtained, and a pilot product of camellia oil traditional Chinese medicine compound capsules is formed;

[0036] (4) Verification: Verify the exact effect of camellia oil on rat intestinal inflammation. Using LPS-induced inflammatory cells and animals as models, and with the help of evaluation parameters, conduct research on the anti-inflammatory function of camellia oil. Analyze the concentration of the inducer, relative cell viability and key enzymes of inflammatory metabolism, and further study the mechanism by which camellia oil regulates intestinal inflammation, so as to provide support and basis for improving the added value of camellia oil.

[0037] (5) Capsule making, encapsulation, and packaging.

[0038] In a preferred preparation method, multi-dose metabolomics analysis is performed on the anti-inflammatory cells and animal experimental models of camellia oil to analyze the anti-inflammatory mechanism of camellia oil from the aspects of inflammatory factor metabolism and lipid metabolism.

[0039] In a preferred preparation method, the tools used in step (2) are SwissTargetPrediction, SEA, Pharmapper, and Superpre.

[0040] In a preferred preparation method, the optimization tool for step (3) is a KEGG and GO analysis optimization tool.

[0041] An application for capsules to relieve enteritis involves combining the anti-inflammatory active ingredients of camellia oil with traditional Chinese medicine ingredients in the preparation of products to relieve human enteritis.

[0042] Example 2: A capsule for relieving enteritis, made from the following components in parts by weight: 35 parts camellia oil, 35 parts salvia miltiorrhiza, 35 parts forsythia suspensa, 35 parts scutellaria baicalensis, 35 parts dandelion, 35 parts honeysuckle, 35 parts amla, 35 parts poria cocos, 35 parts corydalis yanhusuo, 35 parts dendrobium, and 35 parts rehmannia glutinosa.

[0043] A capsule for relieving enteritis, the preparation method of which includes the following steps:

[0044] (1) Compound preparation: Select Salvia miltiorrhiza, Forsythia suspensa, Scutellaria baicalensis, Taraxacum mongolicum, Lonicera japonica, Phyllanthus emblica, Poria cocos, Corydalis yanhusuo, Dendrobium nobile, Rehmannia glutinosa and Camellia chinensis oil for compound preparation;

[0045] (2) Camellia oil component analysis: By using analytical tools, the therapeutic targets of effective components of camellia oil can be comprehensively predicted by utilizing the principles of chemical structure similarity and active functional group pairing. A relevant "camellia oil effective components-anti-inflammatory targets" network and corresponding protein interaction network are constructed.

[0046] (3) By using optimization tools to provide optimization technology for traditional Chinese medicine compound prescriptions, the optimal compound medicinal materials and their proportions are obtained, and a pilot product of camellia oil traditional Chinese medicine compound capsules is formed;

[0047] (4) Verification: Verify the exact effect of camellia oil on rat intestinal inflammation. Using LPS-induced inflammatory cells and animals as models, and with the help of evaluation parameters, conduct research on the anti-inflammatory function of camellia oil. Analyze the concentration of the inducer, relative cell viability and key enzymes of inflammatory metabolism, and further study the mechanism by which camellia oil regulates intestinal inflammation, so as to provide support and basis for improving the added value of camellia oil.

[0048] (5) Capsule making, encapsulation, and packaging.

[0049] In a preferred preparation method, multi-dose metabolomics analysis is performed on the anti-inflammatory cells and animal experimental models of camellia oil to analyze the anti-inflammatory mechanism of camellia oil from the aspects of inflammatory factor metabolism and lipid metabolism.

[0050] In a preferred preparation method, the tools used in step (2) are SwissTargetPrediction, SEA, Pharmapper, and Superpre.

[0051] In a preferred preparation method, the optimization tool for step (3) is a KEGG and GO analysis optimization tool.

[0052] An application for capsules to relieve enteritis involves combining the anti-inflammatory active ingredients of camellia oil with traditional Chinese medicine ingredients in the preparation of products to relieve human enteritis.

[0053] Example 3: A capsule for relieving enteritis, made from the following components in parts by weight: 55 parts camellia oil, 55 parts salvia miltiorrhiza, 55 parts forsythia suspensa, 55 parts scutellaria baicalensis, 55 parts dandelion, 55 parts honeysuckle, 55 parts amla, 55 parts poria cocos, 55 parts corydalis yanhusuo, 55 parts dendrobium, and 55 parts rehmannia glutinosa.

[0054] A capsule for relieving enteritis, the preparation method of which includes the following steps:

[0055] (1) Compound preparation: Select Salvia miltiorrhiza, Forsythia suspensa, Scutellaria baicalensis, Taraxacum mongolicum, Lonicera japonica, Phyllanthus emblica, Poria cocos, Corydalis yanhusuo, Dendrobium nobile, Rehmannia glutinosa and Camellia chinensis oil for compound preparation;

[0056] (2) Camellia oil component analysis: By using analytical tools, the therapeutic targets of effective components of camellia oil can be comprehensively predicted by utilizing the principles of chemical structure similarity and active functional group pairing. A relevant "camellia oil effective components-anti-inflammatory targets" network and corresponding protein interaction network are constructed.

[0057] (3) By using optimization tools to provide optimization technology for traditional Chinese medicine compound prescriptions, the optimal compound medicinal materials and their proportions are obtained, and a pilot product of camellia oil traditional Chinese medicine compound capsules is formed;

[0058] (4) Verification: Verify the exact effect of camellia oil on rat intestinal inflammation. Using LPS-induced inflammatory cells and animals as models, and with the help of evaluation parameters, conduct research on the anti-inflammatory function of camellia oil. Analyze the concentration of the inducer, relative cell viability and key enzymes of inflammatory metabolism, and further study the mechanism by which camellia oil regulates intestinal inflammation, so as to provide support and basis for improving the added value of camellia oil.

[0059] (5) Capsule making, encapsulation, and packaging.

[0060] In a preferred preparation method, multi-dose metabolomics analysis is performed on the anti-inflammatory cells and animal experimental models of camellia oil to analyze the anti-inflammatory mechanism of camellia oil from the aspects of inflammatory factor metabolism and lipid metabolism.

[0061] In a preferred preparation method, the tools used in step (2) are SwissTargetPrediction, SEA, Pharmapper, and Superpre.

[0062] In a preferred preparation method, the optimization tool for step (3) is a KEGG and GO analysis optimization tool.

[0063] An application for capsules to relieve enteritis involves combining the anti-inflammatory active ingredients of camellia oil with traditional Chinese medicine ingredients in the preparation of products to relieve human enteritis.

[0064] Example 4: A capsule for relieving enteritis, made from the following components in parts by weight: 75 parts camellia oil, 75 parts salvia miltiorrhiza, 75 parts forsythia suspensa, 75 parts scutellaria baicalensis, 75 parts dandelion, 75 parts honeysuckle, 75 parts amla, 75 parts poria cocos, 75 parts corydalis yanhusuo, 75 parts dendrobium, and 75 parts rehmannia glutinosa.

[0065] A capsule for relieving enteritis, the preparation method of which includes the following steps:

[0066] (1) Compound preparation: Select Salvia miltiorrhiza, Forsythia suspensa, Scutellaria baicalensis, Taraxacum mongolicum, Lonicera japonica, Phyllanthus emblica, Poria cocos, Corydalis yanhusuo, Dendrobium nobile, Rehmannia glutinosa and Camellia chinensis oil for compound preparation;

[0067] (2) Camellia oil component analysis: By using analytical tools, the therapeutic targets of effective components of camellia oil can be comprehensively predicted by utilizing the principles of chemical structure similarity and active functional group pairing. A relevant "camellia oil effective components-anti-inflammatory targets" network and corresponding protein interaction network are constructed.

[0068] (3) By using optimization tools to provide optimization technology for traditional Chinese medicine compound prescriptions, the optimal compound medicinal materials and their proportions are obtained, and a pilot product of camellia oil traditional Chinese medicine compound capsules is formed;

[0069] (4) Verification: Verify the exact effect of camellia oil on rat intestinal inflammation. Using LPS-induced inflammatory cells and animals as models, and with the help of evaluation parameters, conduct research on the anti-inflammatory function of camellia oil. Analyze the concentration of the inducer, relative cell viability and key enzymes of inflammatory metabolism, and further study the mechanism by which camellia oil regulates intestinal inflammation, so as to provide support and basis for improving the added value of camellia oil.

[0070] (5) Capsule making, encapsulation, and packaging.

[0071] In a preferred preparation method, multi-dose metabolomics analysis is performed on the anti-inflammatory cells and animal experimental models of camellia oil to analyze the anti-inflammatory mechanism of camellia oil from the aspects of inflammatory factor metabolism and lipid metabolism.

[0072] In a preferred preparation method, the tools used in step (2) are SwissTargetPrediction, SEA, Pharmapper, and Superpre.

[0073] In a preferred preparation method, the optimization tool for step (3) is a KEGG and GO analysis optimization tool.

[0074] An application for capsules to relieve enteritis involves combining the anti-inflammatory active ingredients of camellia oil with traditional Chinese medicine ingredients in the preparation of products to relieve human enteritis.

[0075] Example 5: A capsule for relieving enteritis, made from the following components in parts by weight: 95 parts camellia oil, 95 parts salvia miltiorrhiza, 95 parts forsythia suspensa, 95 parts scutellaria baicalensis, 95 parts dandelion, 95 parts honeysuckle, 95 parts amla, 95 parts poria cocos, 95 parts corydalis yanhusuo, 95 parts dendrobium, and 95 parts rehmannia glutinosa.

[0076] A capsule for relieving enteritis, the preparation method of which includes the following steps:

[0077] (1) Compound preparation: Select Salvia miltiorrhiza, Forsythia suspensa, Scutellaria baicalensis, Taraxacum mongolicum, Lonicera japonica, Phyllanthus emblica, Poria cocos, Corydalis yanhusuo, Dendrobium nobile, Rehmannia glutinosa and Camellia chinensis oil for compound preparation;

[0078] (2) Camellia oil component analysis: By using analytical tools, the therapeutic targets of effective components of camellia oil can be comprehensively predicted by utilizing the principles of chemical structure similarity and active functional group pairing. A relevant "camellia oil effective components-anti-inflammatory targets" network and corresponding protein interaction network are constructed.

[0079] (3) By using optimization tools to provide optimization technology for traditional Chinese medicine compound prescriptions, the optimal compound medicinal materials and their proportions are obtained, and a pilot product of camellia oil traditional Chinese medicine compound capsules is formed;

[0080] (4) Verification: Verify the exact effect of camellia oil on rat intestinal inflammation. Using LPS-induced inflammatory cells and animals as models, and with the help of evaluation parameters, conduct research on the anti-inflammatory function of camellia oil. Analyze the concentration of the inducer, relative cell viability and key enzymes of inflammatory metabolism, and further study the mechanism by which camellia oil regulates intestinal inflammation, so as to provide support and basis for improving the added value of camellia oil.

[0081] (5) Capsule making, encapsulation, and packaging.

[0082] In a preferred preparation method, multi-dose metabolomics analysis is performed on the anti-inflammatory cells and animal experimental models of camellia oil to analyze the anti-inflammatory mechanism of camellia oil from the aspects of inflammatory factor metabolism and lipid metabolism.

[0083] In a preferred preparation method, the tools used in step (2) are SwissTargetPrediction, SEA, Pharmapper, and Superpre.

[0084] In a preferred preparation method, the optimization tool for step (3) is a KEGG and GO analysis optimization tool.

[0085] An application for capsules to relieve enteritis involves combining the anti-inflammatory active ingredients of camellia oil with traditional Chinese medicine ingredients in the preparation of products to relieve human enteritis.

[0086] To investigate whether the camellia oil relief capsules of the present invention are effective in improving enteritis, we conducted experimental studies and clinical trials.

[0087] I. Animal Experimental Research

[0088] Experimental methods:

[0089] 1. Select 30 healthy rats and randomly divide them into 5 groups, with 6 rats in each group;

[0090] 2. Group 1 was the control group and the patients were not fed the granules inside the capsules, i.e., they were not fed the anti-inflammatory active ingredients.

[0091] 3. The other groups were experimental groups, fed the granules inside the capsules, i.e., fed the anti-inflammatory active ingredients;

[0092] 4. Group 1 is used for comparison with Groups 2-5; Group 2 is used to observe intestinal morphology; Group 3 is used to observe the apoptosis status of secretory cells; Group 4 is used to observe hormone secretion status; Group 5 is used to observe inflammatory metabolism status; and Groups 2-5 are used to observe survival rate.

[0093]

[0094] II. Clinical Trial Research

[0095] 1. Clinical trials on intestinal morphology

[0096] Experimental Methods: Enteritis patients were recruited as volunteers for a clinical trial. The main method was as follows: 30 volunteers were selected and divided into 5 groups of 6 for observation and statistical analysis. The first group did not use any drugs (comparative group), while the other four groups used the prepared camellia oil capsules.

[0097] Specific treatment method: Oral administration, three times a day, once in the morning, noon and evening, half an hour before meals, one pill each time, can be taken directly or with warm water.

[0098] Statistical method: ① Intestinal symptoms were inquired about once a week; ② Volunteers first underwent intestinal examination using medical imaging technology, and then underwent intestinal examination again using medical imaging technology after four weeks (one course of treatment).

[0099] Statistical data were based on changes in the condition of at least 5 out of 6 people in each group. The results are shown in Table 1.

[0100]

[0101]

[0102] Table 1

[0103] As can be seen from the experimental results in Table 1 above, the use of the camellia oil capsules of the present invention can significantly improve intestinal morphology, which is in line with the expectations of the experimental study.

[0104] 2. Clinical trials on apoptosis

[0105] Experimental Methods: Enteritis patients were recruited as volunteers for a clinical trial. The main method was as follows: 30 volunteers were selected and divided into 5 groups of 6 for observation and statistical analysis. The first group did not use any drugs (comparative group), while the other four groups used the prepared camellia oil capsules.

[0106] Specific treatment method: Oral administration, three times a day, once in the morning, noon and evening, half an hour before meals, one pill each time, can be taken directly or with warm water.

[0107] Statistical method: ① Check the intestinal symptoms once a week; ② Volunteers first have various indicators tested by blood tests, and after four weeks (one course of treatment), the intestinal condition is checked again by blood tests.

[0108] Statistical data were based on changes in the condition of at least 5 out of 6 people in each group. The results are shown in Table 2.

[0109]

[0110]

[0111] Table 2

[0112] As can be seen from the experimental results in Table 2 above, the use of the camellia oil capsules of the present invention can reduce cell apoptosis, which is in line with the expectations of the experimental study.

[0113] 3. Clinical trials on hormone secretion

[0114] Experimental Methods: Enteritis patients were recruited as volunteers for a clinical trial. The main method was as follows: 30 volunteers were selected and divided into 5 groups of 6 for observation and statistical analysis. The first group did not use any drugs (comparative group), while the other four groups used the prepared camellia oil capsules.

[0115] Specific treatment method: Oral administration, three times a day, once in the morning, noon and evening, half an hour before meals, one pill each time, can be taken directly or with warm water.

[0116] Statistical methods: ① Inquiry about intestinal symptoms once a week; ② Volunteers first underwent blood tests to check various indicators, and after four weeks (one course of treatment), they underwent blood tests to check various indicators to check the intestinal condition again.

[0117] Statistical data were based on changes in the condition of at least 5 out of 6 people in each group. The results are shown in Table 3.

[0118]

[0119]

[0120] Table 3

[0121] As can be seen from the experimental results in Table 3 above, the use of the camellia oil capsules of the present invention can increase hormone secretion, which is in line with the expectations of the experimental study.

[0122] 4. Clinical trials on inflammatory metabolism

[0123] Experimental Methods: Enteritis patients were recruited as volunteers for a clinical trial. The main method was as follows: 30 volunteers were selected and divided into 5 groups of 6 for observation and statistical analysis. The first group did not use any drugs (comparative group), while the other four groups used the prepared camellia oil capsules.

[0124] Specific treatment method: Oral administration, three times a day, once in the morning, noon and evening, half an hour before meals, one pill each time, can be taken directly or with warm water.

[0125] Statistical methods: ① Inquiry about intestinal symptoms once a week; ② Volunteers first underwent blood tests to check various indicators, and after four weeks (one course of treatment), they underwent blood tests to check various indicators to check the intestinal condition again.

[0126] Statistical data were based on changes in the condition of at least 5 out of 6 people in each group. The results are shown in Table 4.

[0127]

[0128]

[0129] Table 4

[0130] As can be seen from the experimental results in Table 4 above, the camellia oil capsules of the present invention can inhibit inflammatory metabolism, which is in line with the expectations of the experimental study.

[0131] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A capsule for relieving enteritis, characterized in that, It is made from the following components in parts by weight: 1-100 parts camellia oil, 1-100 parts salvia miltiorrhiza, 1-100 parts forsythia suspensa, 1-100 parts scutellaria baicalensis, 1-100 parts dandelion, 1-100 parts honeysuckle, 1-100 parts amla, 1-100 parts poria cocos, 1-100 parts corydalis yanhusuo, 1-100 parts dendrobium, and 1-100 parts rehmannia glutinosa.

2. The capsule for relieving enteritis according to claim 1, characterized in that, It is made from the following components in parts by weight: 1-100 parts camellia oil, 51-100 parts salvia miltiorrhiza, 51-100 parts forsythia suspensa, 51-100 parts scutellaria baicalensis, 51-100 parts dandelion, 51-100 parts honeysuckle, 1-50 parts amla, 1-50 parts poria cocos, 1-50 parts corydalis yanhusuo, 1-50 parts dendrobium, and 1-50 parts rehmannia glutinosa.

3. The capsule for relieving enteritis according to claim 1, characterized in that, It is made from the following components in parts by weight: 1-100 parts camellia oil, 1-50 parts salvia miltiorrhiza, 1-50 parts forsythia suspensa, 1-50 parts scutellaria baicalensis, 1-50 parts dandelion, 1-50 parts honeysuckle, 51-100 parts amla, 51-100 parts poria cocos, 51-100 parts corydalis yanhusuo, 51-100 parts dendrobium, and 51-100 parts rehmannia glutinosa.

4. The capsule for relieving enteritis according to claim 1, characterized in that, It is made from the following components in parts by weight: 1-100 parts camellia oil, 10-90 parts salvia miltiorrhiza, 10-90 parts forsythia suspensa, 10-90 parts scutellaria baicalensis, 10-90 parts dandelion, 10-90 parts honeysuckle, 10-90 parts amla, 10-90 parts poria cocos, 10-90 parts corydalis yanhusuo, 10-90 parts dendrobium, and 10-90 parts rehmannia glutinosa.

5. A capsule for relieving enteritis according to claim 1, characterized in that, It is made from the following components in parts by weight: 1-100 parts camellia oil, 20-80 parts salvia miltiorrhiza, 20-80 parts forsythia suspensa, 20-80 parts scutellaria baicalensis, 20-80 parts dandelion, 20-80 parts honeysuckle, 20-80 parts amla, 20-80 parts poria cocos, 20-80 parts corydalis yanhusuo, 20-80 parts dendrobium, and 20-80 parts rehmannia glutinosa.

6. A capsule for relieving enteritis according to claim 1, characterized in that, Its preparation method includes the following steps: (1) Compound preparation: Select Salvia miltiorrhiza, Forsythia suspensa, Scutellaria baicalensis, Taraxacum mongolicum, Lonicera japonica, Phyllanthus emblica, Poria cocos, Corydalis yanhusuo, Dendrobium nobile, Rehmannia glutinosa and Camellia chinensis oil for compound preparation; (2) Camellia oil component analysis: By using analytical tools, the therapeutic targets of effective components of camellia oil can be comprehensively predicted by utilizing the principles of chemical structure similarity and active functional group pairing. A relevant "camellia oil effective components-anti-inflammatory targets" network and corresponding protein interaction network are constructed. (3) By using optimization tools to provide optimization technology for traditional Chinese medicine compound prescriptions, the optimal compound medicinal materials and their proportions are obtained, and a pilot product of camellia oil traditional Chinese medicine compound capsules is formed; (4) Verification: Verify the exact effect of camellia oil on rat intestinal inflammation. Using LPS-induced inflammatory cells and animals as models, and with the help of evaluation parameters, conduct research on the anti-inflammatory function of camellia oil. Analyze the concentration of the inducer, relative cell viability and key enzymes of inflammatory metabolism, and further study the mechanism by which camellia oil regulates intestinal inflammation, so as to provide support and basis for improving the added value of camellia oil. (5) Capsule making, encapsulation, and packaging.

7. The method for preparing a capsule for relieving enteritis according to claim 6, characterized in that, The study conducted multi-dose metabolomics analysis on the anti-inflammatory effects of camellia oil in cell and animal experimental models, analyzing the anti-inflammatory mechanism of camellia oil from the aspects of inflammatory factor metabolism and lipid metabolism.

8. A method for preparing a capsule for relieving enteritis according to claim 6, characterized in that, The tools used in step (2) are SwissTargetPrediction, SEA, Pharmapper, and Superpre.

9. A method for preparing a capsule for relieving enteritis according to claim 6, characterized in that, The optimization tool in step (3) is the KEGG and GO analysis and optimization tool.

10. An application of a capsule for relieving enteritis, characterized in that, The anti-inflammatory active ingredients of camellia oil are combined with traditional Chinese medicine ingredients and applied to the preparation of products that relieve human enteritis.

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