Skin care polypeptide composition with synergistic whitening effect and skin care product containing skin care polypeptide composition

Abstract

The invention discloses a skincare polypeptide composition with a synergistic whitening effect and a skincare product containing the skincare polypeptide composition. The skin care polypeptide composition is prepared from the following components in percentage by mass: 50 to 70 percent of two-row yeast fermentation product filtrate, 1 to 3 percent of saccharomycetes fermentation product, 0.7 to 1 percent of composite peptide, 1 to 3 percent of hydroxyethyl piperazine ethanesulfonic acid, 0.3 to 0.8 percent of 1, 2-hexanediol, 0.3 to 0.7 percent of p-hydroxyacetophenone, 8 to 12 percent of 1, 3-butanediol and the balance of water, the composite peptide is glutathione, oligopeptide-1, carnosine and argireline (acetyl hexapeptide-8); the mass ratio of the glutathione to the oligopeptide-1 to the carnosine to the argireline (acetyl hexapeptide-8) is (0.05 to 0.15) to (1 to 3) to (3 to 8) to (0.5 to 1.5). According to the invention, the percutaneous penetration of polypeptide can be well improved, so that carnosine, argireline (acetyl hexapeptide-8) and other whitening efficacy raw materials can penetrate into a basal layer, the generation of melanin can be better blocked, and a better whitening efficacy can be played.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and particularly relates to a skin care polypeptide composition with synergistic whitening effect and a skin care product containing the same. BACKGROUND

[0002] In recent years, the source of functional raw materials for cosmetics has gradually transitioned from chemical synthesis and plant extraction to biological and genetic beauty. Among them, the peptide functional raw materials composed of specific amino acid sequences have shown great potential in the field of cosmetics due to their high biological activity, small addition amount, clear mechanism, and various advantages such as whitening, freckle removal, anti-aging, and wound repair. Skin pigmentation is one of the main problems affecting the appearance of the skin, and its formation mechanism is mainly related to abnormal synthesis and metabolism of melanin. Melanin is generated in melanocytes through a tyrosinase-catalyzed oxidation reaction, so inhibiting the activity of tyrosinase is one of the key strategies for skin whitening.

[0003] At present, a variety of whitening active ingredients are widely used in cosmetics, among which peptide ingredients have attracted widespread attention due to their good biocompatibility and specific action mechanism, such as carnosine, hexapeptide-8 (acetyl hexapeptide-8), glutathione, and oligopeptide-1. However, due to the large molecular weight and strong hydrophilicity of peptides, their transdermal penetration ability is poor, and they are difficult to effectively reach the skin basal layer to block melanin production, which limits the full play of their whitening effect. Therefore, developing a composition that can effectively promote the transdermal absorption of peptide active ingredients and achieving synergistic whitening effect on this basis has become a technical problem to be solved in the field.

[0004] Although some penetration enhancers are used in the prior art, most of them have problems such as strong irritation, limited penetration effect, or poor compatibility with active ingredients. Therefore, it is of great significance to provide a safe and effective composition that can significantly improve the transdermal absorption of polypeptide ingredients and play a synergistic whitening effect. SUMMARY

[0005] In order to overcome the shortcomings of the prior art, the purpose of the present application is to provide a skin care polypeptide composition with synergistic whitening effect, which can significantly improve the transdermal penetration of polypeptides and make the whitening active ingredients penetrate into the basal layer to effectively block the production of melanin.

[0006] Another purpose of the present application is to provide a preparation method of the skin care polypeptide composition.

[0007] Still another purpose of the present application is to provide the application of the skin care polypeptide composition in cosmetics.

[0008] To achieve the above purposes, the present application adopts the following technical solutions: In a first aspect, the present application provides a skin care polypeptide composition with synergistic whitening effect, consisting of the following ingredients by mass percentage: The filtrate of Saccharomyces boulardii fermentation product 50-70%, the fermentation product of Saccharomyces 1-3%, the complex peptide 0.7-1%, hydroxyethyl piperazine ethane sulfonic acid 1-3%, 1,2-hexanediol 0.3-0.8%, p-hydroxyacetophenone 0.3-0.5%, 1,3-butanediol 8-12%, and water in the remainder.

[0009] The complex peptide is a mixture of glutathione, oligopeptide-1, carnosine and hexapeptide-8 (acetyl hexapeptide-8).

[0010] Preferably, the mass ratio of glutathione, oligopeptide-1, carnosine and hexapeptide-8 (acetyl hexapeptide-8) is 0.05-0.15:1-3:3-8:0.5-1.5; further preferably 0.1:2:5:1.

[0011] Preferably, the mass ratio of the complex peptide to hydroxyethyl piperazine ethane sulfonic acid, 1,2-hexanediol and 1,3-butanediol is 0.755-0.965:1-3:0.3-0.8:8-12; further preferably 0.81:2:0.5:10.

[0012] In a most preferred embodiment, the skin care polypeptide composition comprises, by mass percentage: the filtrate of Saccharomyces boulardii fermentation product 70%, the fermentation product of Saccharomyces 2%, the complex peptide 0.81%, hydroxyethyl piperazine ethane sulfonic acid 2%, 1,2-hexanediol 0.5%, p-hydroxyacetophenone 0.5%, 1,3-butanediol 10%, and water in the remainder; wherein the complex peptide is composed of glutathione, oligopeptide-1, carnosine and hexapeptide-8 in a mass ratio of 0.1:2:5:1.

[0013] In a second aspect, the present application provides a preparation method of the above-mentioned skin care polypeptide composition, comprising the following steps: mixing the filtrate of Saccharomyces boulardii fermentation product, the fermentation product of Saccharomyces, the complex peptide, hydroxyethyl piperazine ethane sulfonic acid, 1,2-hexanediol, p-hydroxyacetophenone, 1,3-butanediol and water in the formula amount, stirring uniformly until completely dissolved.

[0014] In a third aspect, the present application provides the use of hydroxyethyl piperazine ethane sulfonic acid, 1,2-hexanediol and 1,3-butanediol in combination in the preparation of a penetration enhancer for peptide macromolecular active ingredients.

[0015] The peptide macromolecular active ingredients include glutathione, oligopeptide-1, carnosine and hexapeptide-8 (acetyl hexapeptide-8). Preferably, the mass ratio is 0.05-0.15:1-3:3-8:0.5-1.5.

[0016] This invention discovers that the combination of hydroxyethylpiperazine ethanesulfonic acid (a mild buffer and keratolytic agent), 1,2-hexanediol (a polyol with both moisturizing and antiseptic functions), and 1,3-butanediol (an excellent moisturizer) can produce an unexpected synergistic penetration-enhancing effect, significantly promoting the penetration of peptide macromolecules into the skin barrier and allowing them to reach the basal layer to exert their effects.

[0017] Fourthly, the present invention provides the application of the above-mentioned skincare polypeptide composition in the preparation of cosmetics. The cosmetics include, but are not limited to, facial cleansers, face masks, scrubs, hand creams, body lotions, essences, gels, and serums.

[0018] Fifthly, the present invention provides a cosmetic comprising the above-described skincare polypeptide composition and cosmetically acceptable excipients.

[0019] Compared with the prior art, the present invention has the following beneficial effects: 1) Innovative penetration enhancement system: For the first time, hydroxyethylpiperazine ethanesulfonic acid, 1,2-hexanediol and 1,3-butanediol are combined as a penetration enhancer for peptide macromolecules. This combination is safe and mild, and can effectively solve the industry pain point of difficult transdermal absorption of peptide ingredients.

[0020] 2) Multi-target synergistic whitening: The Bifida ferment filtrate, yeast ferment products and four functional peptides (glutathione, oligopeptide-1, carnosine and hexapeptide) in the composition work synergistically to target multiple links in melanin production (inhibiting tyrosinase activity, anti-oxidation, anti-glycation, and promoting keratin renewal) to achieve multi-pathway whitening.

[0021] 3) Synergistic effect: Through specific ratios, each active ingredient and the penetration-enhancing system produce a significant synergistic effect, making the whitening effect of the final composition far superior to that of a single ingredient or simple superposition.

[0022] 4) High safety: Most of the selected ingredients are of natural origin or bio-fermentation products, the formula is mild and has low skin irritation. Detailed Implementation

[0023] This invention is not to be limited to the embodiments shown herein, but can be applied to a wider range consistent with the principles and novel features disclosed herein. While any methods and materials similar to or equivalent to those described herein may be used in the implementation or testing of this invention, preferred methods and materials are set forth herein.

[0024] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0025] This invention provides a skincare polypeptide composition with synergistic whitening effects, which, by mass percentage, consists of the following components: The composition includes 50-70% of the fermentation product filtrate of Diplosperidium oxychloride, 1-3% of yeast fermentation product, 0.7-1% of complex peptides, 1-3% of hydroxyethylpiperazine ethanesulfonic acid, 0.3-0.8% of 1,2-hexanediol, 0.3-0.5% of p-hydroxyacetophenone, 8-12% of 1,3-butanediol, and water as the balance; the complex peptides are glutathione, oligopeptide-1, carnosine, and hexapeptide (acetyl hexapeptide-8).

[0026] In some embodiments, the mass ratio of glutathione, oligopeptide-1, carnosine, and hexapeptide (acetyl hexapeptide-8) is 0.05-0.15:1-3:3-8:0.5-1.5; preferably 0.1:2:5:1.

[0027] In some embodiments, the mass ratio of the complex peptide to hydroxyethylpiperazine ethanesulfonic acid, 1,2-hexanediol and 1,3-butanediol is 0.755-0.965:1-3:0.3-0.8:8-12; preferably 0.81:2:0.5:10.

[0028] In some embodiments, the total mass ratio of the complex peptide to hydroxyethylpiperazine ethanesulfonic acid, 1,2-hexanediol and 1,3-butanediol is 0.755-0.965:11.8-13.3; preferably 0.81:12.5.

[0029] In some embodiments, the skin care peptide composition comprises, by weight percentage, 70% of Bacillus subtilis ferment filtrate, 2% of yeast ferment product, 0.81% of complex peptide, 2% of hydroxyethylpiperazine ethane sulfonic acid, 0.5% of 1,2-hexanediol, 0.5% of p-hydroxyacetophenone, 10% of 1,3-butanediol, and the balance being water. The complex peptide is glutathione, oligopeptide-1, carnosine, and hexapeptide (acetyl hexapeptide-8); the mass ratio of glutathione, oligopeptide-1, carnosine, and hexapeptide (acetyl hexapeptide-8) is 0.1: 2: 5: 1.

[0030] Furthermore, the present invention also provides the application of the above-mentioned skin-care polypeptide composition in the preparation of cosmetics. In some embodiments, the skin-care polypeptide composition of the present invention can also be used as a whitening ingredient in cosmetics. In some embodiments, the cosmetics include facial cleansers, facial masks, scrubs, hand creams, body lotions, essences, gels, and serums. Fifthly, the present invention also provides a cosmetic containing the above-mentioned skin-care polypeptide composition. In some embodiments, the cosmetic includes the above-mentioned skin-care polypeptide composition and excipients.

[0031] This invention utilizes hydroxyethylpiperazine ethanesulfonic acid, 1,2-hexanediol, and 1,3-butanediol as a combined penetration enhancer, which effectively improves the transdermal penetration of peptide macromolecular active ingredients. This allows whitening ingredients such as Bifida ferment filtrate, yeast ferment products, glutathione, oligopeptide-1, carnosine, and hexapeptide (acetyl hexapeptide-8) to penetrate deeper into the basal layer, better blocking melanin production and exerting a superior whitening effect.

[0032] Experiments have shown that the combination of the yeast fermentation product filtrate, yeast fermentation product, complex peptide, hydroxyethylpiperazine ethanesulfonic acid, 1,2-hexanediol and 1,3-butanediol of the present invention produces a synergistic effect in whitening.

[0033] The present invention will be further described in detail below through examples and test cases, but the scope of protection of the present invention is not limited thereto.

[0034] Examples 1 to 5 Examples 1-5 provide five different ratios of skin care peptide compositions, the specific composition and ratio of which are shown in Table 1 below.

[0035] Table 1. Formulations of skincare peptide compositions in Examples 1-5 (percentage by mass)

[0036] Complex peptide composition (mass ratio): Examples 1, 4, and 5: Glutathione:Oligopeptide-1:Carnosine:Hexapeptide (Acetylhexapeptide-8) = 0.1:2:5:1; Example 2: Glutathione:Oligopeptide-1:Carnosine:Hexapeptide (Acetylhexapeptide-8) = 0.05:3:3:1.5; Example 3: Glutathione:Oligopeptide-1:Carnosine:Hexapeptide (acetylhexapeptide-8) = 0.15:1:8:0.5.

[0037] Preparation method: Weigh each component according to the proportions shown in Table 1, place them in a mixing container, and stir at room temperature until completely dissolved and mixed evenly to obtain the skin care peptide composition.

[0038] Comparative Example 1 A complex peptide solution without penetration enhancers: 0.81% complex peptides (glutathione: oligopeptide-1:carnosine: hexapeptide (acetyl hexapeptide-8) = 0.1:2:5:1), made up to 100% with water, prepared in the same way as in Example 1.

[0039] Comparative Example 2 Contains only 1,3-butanediol as a penetration enhancer: 0.81% complex peptide (same ratio as in Example 1), 10% 1,3-butanediol, 0.5% p-hydroxyacetophenone, and water to make up to 100%.

[0040] Comparative Example 3 A combination of commercially available penetration enhancers (0.5% azone): 0.81% complex peptide (same ratio as in Example 1), 0.5% azone, 10% 1,3-butanediol, 0.5% p-hydroxyacetophenone, and water to make up to 100%.

[0041] The following content pertains to the efficacy trial cases.

[0042] Experimental Example 1: Transdermal Absorption Test (In Vitro Method) The experiment was conducted according to the in vitro diffusion cell method provided in the background art, using the composition of Example 1 as the supply solution and Comparative Examples 1-3 as controls. The concentration of carnosine in the receiving solution was measured, and the cumulative permeation per unit area (Q_n, μg / cm²) was calculated.

[0043] The results are shown in Table 2.

[0044] Table 2. Cumulative permeation of carnosine per unit area after 10 hours (n=3, mean ± SD)

[0045] The results showed that the permeation-enhancing effect of the composition of Example 1 of the present invention was significantly better than that of the control group without a permeation enhancer (Comparative Example 1), the control group containing only a single permeation enhancer (Comparative Example 2), and the control group containing the commercially available permeation enhancer azone (Comparative Example 3) (p<0.01).

[0046] Experimental Example 2: Tyrosinase Inhibition Rate Test Experimental samples: Sample group (in this invention): The composition prepared in Example 1. Control group 1: An aqueous solution containing only 0.81% complex peptide (with the same ratio as in Example 1).

[0047] Control group 2: An aqueous solution containing 0.81% complex peptide and 2% hydroxyethylpiperazine ethanesulfonic acid.

[0048] Positive control: 2% arbutin aqueous solution.

[0049] Blank control: Phosphate-buffered saline (PBS).

[0050] Experimental Methods: The L-DOPA method was used. Brief Procedure: PBS, sample solutions of different concentrations, and tyrosinase solution were added sequentially to a 96-well plate. The plate was incubated at 37°C for 10 minutes, then L-DOPA solution was added. The absorbance (OD) value was immediately measured at 475 nm, and the kinetic curve was continuously monitored. The tyrosinase inhibition rate of each sample was calculated. Inhibition rate (%) = (1 - ΔODsample / ΔODblank) × 100%. Experimental Results: The results are shown in Table 3. Table 3: Inhibition rate of tyrosinase by each sample (n=3, mean±SD)

[0051] As can be seen from the results in Table 3 above, the skin-care peptide composition of the present invention (sample group) exhibits extremely strong tyrosinase inhibition ability, with an inhibition rate significantly higher than that of a single complex peptide solution (control group 1) and a combination of complex peptide and some penetration enhancers (control group 2), and even superior to the classic whitening ingredient arbutin. This indicates that a strong synergistic inhibitory effect is generated among the various components (diester yeast filtrate, yeast fermentation products, complex peptides, etc.) in the composition of the present invention.

[0052] Experimental Example 3: Validation of Synergistic Whitening Effect (Cellular Level) 1. Experimental Samples: Group A (Full Formulation Group): The composition prepared in Example 1 (including the penetration enhancement system).

[0053] Group B (no penetration enhancer group): Contains no hydroxyethylpiperazine ethanesulfonic acid, 1,2-hexanediol, or 1,3-butanediol, and consists only of Diplosacchariformis fermentation product filtrate, yeast fermentation product, complex peptides, and water. The content of each active ingredient is the same as in Example 1.

[0054] Group C (Yeast-free group): Contains no Bifida ferment filtrate or yeast fermentation products, and consists only of complex peptides, penetration enhancers (HEPES, 1,2-hexanediol, 1,3-butanediol) and water. The content of complex peptides and penetration enhancers is the same as in Example 1.

[0055] Group D (Single Peptide Group): An aqueous solution containing only 0.81% carnosine (the single peptide with the highest content among complex peptides) and a penetration enhancer.

[0056] Group E (blank control group): Cell culture medium.

[0057] 2. Experimental Methods: A human epidermal melanocyte model was used. A melanin production model was established by stimulating cells with α-melanocyte-stimulating hormone (α-MSH). After intervention with different samples mentioned above, cells were cultured for another 72 hours. Cells were collected, and intracellular melanin content and tyrosinase activity were measured.

[0058] 3. Experimental Results: The results are shown in Table 4 (relative values ​​were calculated with the blank control group as 100%).

[0059] Table 4: Effects of each sample on α-MSH-induced melanin production and tyrosinase activity (n=3, mean ±SD)

[0060] As can be seen from the results in Table 4 above, the whitening effect (reduction of melanin and tyrosinase activity) of the full formula group (Group A) is far superior to any other group, proving that the formula of the present invention has a significant synergistic effect.

[0061] Comparing group A and group B (without penetration enhancer), it is evident that the penetration enhancement system of the present invention is crucial for the efficacy of active ingredients and can significantly improve their intracellular bioavailability.

[0062] Comparing group A and group C (without yeast), it is evident that there is a clear synergistic effect between the yeast fermentation product filtrate and the yeast fermentation product and the complex peptide, which jointly contribute to the final whitening effect.

[0063] Group A showed significantly better results than Group D (single peptide), demonstrating the superiority of compound peptide formulations.

[0064] In summary, this invention, through its unique combination and ratio of ingredients, and especially its innovative use of a specific penetration-enhancing system, enables the entire composition to exhibit outstanding and synergistic whitening effects in both inhibiting tyrosinase, a key target for melanin production, and in the overall cellular whitening model. This solves the industry problem of peptide ingredients being difficult to penetrate the skin and having insufficient efficacy.

[0065] Examples of cosmetic preparation and efficacy tests of skin care peptide compositions containing the present invention are as follows.

[0066] Application Example 1: Formulation of Whitening Essence Lotion (wt%): The skin care peptide composition of Example 1: 10.0%; hydrogenated polyisobutylene: 8.0%; glycerin: 5.0%; cetearyl alcohol: 3.0%; squalane: 2.0%; tocopheryl acetate: 0.5%; carbomer: 0.3%; triethanolamine: 0.2%; fragrance: 0.1%; deionized water: balance to 100%.

[0067] Preparation method: Oil phase preparation: Hydrogenated polyisobutylene, cetearyl alcohol, squalane and tocopheryl acetate are mixed, heated to 75–80°C and stirred until completely dissolved.

[0068] Aqueous phase preparation: Add glycerol and carbomer to deionized water, heat to 75-80℃, and stir until the carbomer is completely swollen.

[0069] Emulsification: The oil phase is slowly added to the aqueous phase under high-speed homogenization, and the mixture is homogenized and emulsified for 5 minutes, and then the temperature is slowly lowered to 45°C.

[0070] Add active ingredients: Add the skin care peptide composition and stir well.

[0071] pH adjustment and post-treatment: Add triethanolamine to adjust the pH to 5.5–6.0, add flavoring, homogenize for 2 minutes, cool to room temperature and then discharge.

[0072] Application Example 2: Preparation formula of whitening facial mask (mass percentage, wt%) The skincare peptide composition of Example 1: 5.0%; xanthan gum: 0.5%; sodium hyaluronate: 0.2%; glycerin: 3.0%; butylene glycol: 3.0%; p-hydroxyacetophenone (additional preservative): 0.2%; deionized water: balance to 100%. Preparation method: Swelling colloid: Slowly add xanthan gum to a portion of deionized water and stir until completely swollen.

[0073] Dissolve the moisturizer: Add glycerin and butylene glycol, and stir until well combined. Add the active ingredients: Add the skin-care peptide composition and sodium hyaluronate, and stir until completely dissolved.

[0074] Volume adjustment and preservation: Add the remaining deionized water to 100% of the total volume, add p-hydroxyacetophenone, stir evenly, let stand to defoam, and fill into the mask cloth.

[0075] Example 3: Preparation formula of whitening essence water (mass percentage, wt%) The skincare peptide composition of Example 1: 8.0%; 1,3-Butanediol: 5.0%; Niacinamide: 2.0%; Allantoin: 0.1%; Citric acid: 0.05%; Sodium citrate: 0.1%; Deionized water: balance to 100%; Preparation method: Dissolution: Dissolve allantoin, citric acid and sodium citrate in a portion of deionized water.

[0076] Add active ingredients: Add the skin care peptide composition, 1,3-butanediol and niacinamide in sequence, and stir until completely dissolved.

[0077] Volume adjustment and pH adjustment: Add the remaining deionized water to 100% of the total volume, adjust the pH to 5.0–5.5 using a citric acid / sodium citrate buffer system, filter, and then fill.

[0078] Cosmetic efficacy test examples Experimental Example 1: Evaluation of Skin Whitening Efficacy in Humans (In Vitro Melanin Inhibition Assay) Experimental methods: Human skin models (such as the MatTek epidermal model) were used, and the following experimental groups were set up: Experimental group: Essence lotion containing 10% skin care peptide composition; Positive control group: serum containing 2% arbutin; Negative control group: Basic formula (without active ingredients).

[0079] Samples were applied to each model continuously for 7 days, once a day. Samples were taken on day 0 and day 7 to detect melanin content and tyrosinase activity.

[0080] Results: The melanin content in the experimental group was reduced by 45.2% compared with the negative control group, and the tyrosinase activity inhibition rate reached 48.7%; the melanin content in the positive control group (arbutin) was reduced by 32.5%; indicating that the skin care peptide composition of the present invention has a significantly better synergistic whitening effect than traditional whitening ingredients.

[0081] Experimental Example 2: Skin Permeability Evaluation (Franz Diffusion Cell Method) Experimental method: Pigskin was used as a skin model, and the following experimental groups were set up: Experimental group: containing skin care peptide composition (containing penetration enhancers hydroxyethylpiperazine ethanesulfonic acid, 1,2-hexanediol, and 1,3-butanediol). Control group: The same peptide composition without penetration enhancer. The cumulative penetration of glutathione, hexapeptide, and other components was measured over 24 hours.

[0082] Results: In the experimental group, the skin penetration rate of glutathione increased by 3.2 times and the penetration rate of hexapeptide increased by 2.8 times; demonstrating that the penetration enhancer system significantly improves the transdermal absorption efficiency of peptide active ingredients.

[0083] Trial Example 3: Consumer Clinical Assessment Subjects: 30 women aged 25–45 with dull skin and pigmentation problems.

[0084] Experimental protocol: Use a whitening essence containing 5% skin care peptide composition every morning and evening for 28 consecutive days.

[0085] Assessment metrics: Melanin index (MI) was measured using a skin colorimeter (Mexameter®) and combined with expert visual assessment.

[0086] Results: After 28 days of use, the average melanin index of the subjects decreased by 18.6%; 95% of the subjects reported brighter skin tone and lighter pigmentation; no skin irritation or allergic reactions were reported.

[0087] In conclusion, the above preparation examples demonstrate that this skincare peptide composition can be widely used in formulations such as serums, masks, and essences.

[0088] Efficacy tests have demonstrated that it significantly enhances the skin permeability of whitening active ingredients through a penetration enhancer system, and achieves highly effective and safe whitening results through the synergistic effect of multiple peptides and fermentation products.

[0089] The above experimental results show that the skin care peptide composition provided by the present invention, through a specific combination of Diplosacchariformis fermentation product filtrate, yeast fermentation product, hydroxyethylpiperazine ethanesulfonic acid, 1,2-hexanediol and 1,3-butanediol as a penetration-enhancing system, greatly improves the transdermal delivery efficiency of various whitening peptides.

[0090] More importantly, this composition is not simply a mixture of active ingredients and penetration enhancers. Instead, through the ingenious ratio of each component, it significantly enhances the inhibitory activity against tyrosinase and the inhibition of melanin production while delivering the ingredients efficiently, resulting in a synergistic whitening effect of "1+1+1+1>4". It is also highly safe and has broad prospects for cosmetic applications.

[0091] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A skin care polypeptide composition having synergistic whitening efficacy, characterized in that, By mass percent, the following components are included: Compound peptide 0.7-1%; Saccharomyces boulardii fermentate filtrate 50-70%; Yeast fermentate 1-3%; Hydroxyethylpiperazineethanesulfonic acid 1-3%; 1,2-Hexanediol 0.3-0.8%; p-Hydroxyacetophenone 0.3-0.5%; 1,3-Butanediol 8-12%; Water balance; The compound peptide is composed of glutathione, oligopeptide-1, carnosine and hexapeptide-8 (acetyl hexapeptide-8), and the mass ratio of glutathione, oligopeptide-1, carnosine and hexapeptide-8 (acetyl hexapeptide-8) is 0.05-0.15:1-3:3-8:0.5-1.

5.

2. The skin care polypeptide composition of claim 1, characterized in that: The mass ratio of glutathione, oligopeptide-1, carnosine and hexapeptide-8 (acetyl hexapeptide-8) is 0.1:2:5:

1.

3. The skin care polypeptide composition of claim 1, wherein: The mass ratio of the compound peptide, hydroxyethylpiperazineethanesulfonic acid, 1,2-hexanediol and 1,3-butanediol is 0.755-0.965:1-3:0.3-0.8:8-12.

4. The skin care polypeptide composition of claim 3, wherein: The mass ratio of the compound peptide, hydroxyethylpiperazineethanesulfonic acid, 1,2-hexanediol and 1,3-butanediol is 0.81:2:0.5:

10.

5. The skin care polypeptide composition of claim 4, wherein: The ratio of the total mass of the compound peptide, hydroxyethylpiperazineethanesulfonic acid, 1,2-hexanediol and 1,3-butanediol is 0.755-0.965:11.8-13.

3.

6. The skin care polypeptide composition of claim 5, wherein: The ratio of the total mass of the compound peptide, hydroxyethylpiperazineethanesulfonic acid, 1,2-hexanediol and 1,3-butanediol is 0.81:12.

5.

7. The skin care polypeptide composition of claim 1, wherein, By mass percent, the following components are included: Compound peptide 0.7-1%; Saccharomyces boulardii fermentate filtrate 50-70%; Yeast fermentate 1-3%; Hydroxyethylpiperazineethanesulfonic acid 1-3%; 1,2-Hexanediol 0.3-0.8%; p-Hydroxyacetophenone 0.3-0.5%; 1,3-Butanediol 8-12%; Water balance; The compound peptide is composed of glutathione, oligopeptide-1, carnosine and hexapeptide-8 (acetyl hexapeptide-8), and the mass ratio of glutathione, oligopeptide-1, carnosine and hexapeptide-8 (acetyl hexapeptide-8) is 0.05-0.15:1-3:3-8:0.5-1.

5.

8. A method of preparing a skin care polypeptide composition according to any one of claims 1 to 7, characterized in that, The following steps are included: Mix the formula amount of saccharomyces boulardii fermentate filtrate, yeast fermentate, compound peptide, hydroxyethylpiperazineethanesulfonic acid, 1,2-hexanediol, p-hydroxyacetophenone, 1,3-butanediol and water uniformly; hydroxyethylpiperazineethanesulfonic acid, 1,2-hexanediol and 1,3-butanediol are combined for use in the preparation of a penetration enhancer for peptide macromolecular active ingredients, which include glutathione, oligopeptide-1, carnosine and hexapeptide-8 (acetyl hexapeptide-8); The mass ratio of glutathione, oligopeptide-1, carnosine and hexapeptide-8 (acetyl hexapeptide-8) is 0.05-0.15:1-3:3-8:0.5-1.5; the mass ratio of glutathione, oligopeptide-1, carnosine and hexapeptide-8 (acetyl hexapeptide-8) is 0.1:2:5:1; The mass ratio of the compound peptide, hydroxyethylpiperazineethanesulfonic acid, 1,2-hexanediol and 1,3-butanediol is 0.755-0.965:1-3:0.3-0.8:8-12. The ratio of the total mass of the complex peptide, hydroxyethylpiperazine ethane sulfonic acid, 1,2-hexanediol and 1,3-butanediol is 0.755-0.965:11.8-13.3; the ratio of the total mass of the complex peptide, hydroxyethylpiperazine ethane sulfonic acid, 1,2-hexanediol and 1,3-butanediol is 0.81:12.5; the mass ratio of the complex peptide, hydroxyethylpiperazine ethane sulfonic acid, 1,2-hexanediol and 1,3-butanediol is 0.81:2:0.5:

10.

9. Use of the skin care polypeptide composition according to any one of claims 1-7 in the preparation of a cosmetic product, characterized in that: The cosmetic product comprises facial cleanser, facial mask, scrub, hand cream, body lotion, essence, jelly and essence cream.

10. A skin care product comprising: The skin care polypeptide composition according to any one of claims 1-7 and adjuvants.

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