Liquid modified milk and preparation method thereof

Abstract

The invention relates to liquid modified milk and a preparation method thereof, the liquid modified milk comprises animal milk, glucosamine hydrochloride, calcium beta-hydroxy-beta-methylbutyrate and cordyceps militaris extract, the cordyceps militaris extract contains cordycepin and adenosine, the cordycepin content in the liquid modified milk is greater than or equal to 1.6 mg / 100mL, and the adenosine content in the liquid modified milk is greater than or equal to 0.6 mg / 100mL. According to the modified milk provided by the invention, cordycepin enhances the biosynthesis and energy supply capability of mitochondria by activating related signal channels, and provides an energy basis for the GaHMB to play a role, so that the synthesis efficiency of muscle protein is improved; adenosine and glucosamine have a synergistic effect, so that conduction of inflammation-related signals can be blocked, and decomposition of cartilage matrixes is reduced; meanwhile, glucosamine is used as a glycosaminoglycan precursor, under the action of a signal channel regulated and controlled by adenosine, the synthesis efficiency of cartilage tissue can be remarkably stimulated, the utilization efficiency of sugar is improved, and finally triple synergistic intervention of muscle-joint-metabolism is achieved.

Description

Technical Field

[0001] This invention relates to the field of dairy beverages, and more specifically, to a liquid modified milk and its preparation method. Background Technology

[0002] The United Nations predicts that by 2050, the global population aged 60 and over will account for 21% of the total population. Against the backdrop of an accelerating global population aging, age-related diseases are exhibiting a comorbidity of muscle-joint-metabolic factors: the incidence of sarcopenia in people over 60 exceeds 30%, leading to a threefold increase in the risk of falls; and the prevalence of osteoarthritis in people over 65 is greater than 50%. Therefore, the accelerating global aging process is creating an urgent need for products that synergistically support muscle and joint health. Developing a high-protein modified milk product specifically designed for middle-aged and elderly people, simultaneously addressing the needs of sarcopenia and osteoarthritis health, will effectively fill the market gap for functional liquid milk. Summary of the Invention

[0003] One aspect of the present invention relates to a liquid modified milk comprising: animal milk, glucosamine hydrochloride, calcium β-hydroxy-β-methylbutyrate, and Cordyceps militaris extract, wherein the Cordyceps militaris extract contains cordycepin and adenosine, and the liquid modified milk contains ≥1.6 mg / 100 mL of cordycepin and ≥0.6 mg / 100 mL of adenosine.

[0004] In one embodiment, based on 1000 parts by weight of animal milk, the amount of glucosamine hydrochloride added is 0.5 to 3.0 parts, the amount of calcium β-hydroxy-β-methylbutyrate added is 0.5 to 3.0 parts, and the amount of Cordyceps militaris extract added is 0.2 to 1.0 parts.

[0005] In one embodiment, the protein content of the modified milk is 5.8%-6.2%.

[0006] In one embodiment, the modified milk further includes a stabilizer, which includes two or more of the following: mono- and diglycerides of fatty acids, microcrystalline cellulose, sodium alginate, xanthan gum, and sodium carboxymethyl cellulose.

[0007] In one embodiment, the mass ratio of animal milk to stabilizer is (850-900):(2-4).

[0008] In one embodiment, the modified milk further includes a sweetener, which includes at least one of white sugar, sucralose, or acesulfame potassium.

[0009] Preferably, the mass ratio of animal milk to sweetener is (850-900):(35-55).

[0010] One aspect of the present invention relates to a method for preparing a liquid modified milk, comprising: homogenizing and sterilizing animal milk to obtain a sterilized base material; and, under aseptic conditions, adding a sterile solution containing glucosamine hydrochloride, a sterile solution containing calcium β-hydroxy-β-methylbutyrate, and a sterile solution containing Cordyceps militaris extract to the sterilized base material, wherein the Cordyceps militaris extract contains cordycepin and adenosine, and the liquid modified milk contains ≥1.6 mg / 100 mL of cordycepin and ≥0.6 mg / 100 mL of adenosine.

[0011] In one embodiment, glucosamine hydrochloride solution, calcium β-hydroxy-β-methylbutyrate solution, and Cordyceps militaris extract solution are sequentially injected into cooled sterilized substrate at flow rates of 10-15 L / min, 8-12 L / min, and 5-8 L / min, respectively.

[0012] In one embodiment, based on parts by mass, with animal milk as 1000 parts, the amount of aseptic solution of glucosamine hydrochloride added is 0.5 to 3.0 parts, the amount of aseptic solution of calcium β-hydroxy-β-methylbutyrate added is 0.5 to 3.0 parts, and the amount of aseptic solution of Cordyceps militaris extract added is 0.2 to 1.0 parts.

[0013] In one embodiment, prior to homogenization and sterilization of the animal milk, the process includes: RO membrane concentration of the animal milk, resulting in a protein content of 5.8%-6.2% after membrane concentration.

[0014] In one embodiment, Cordyceps militaris extract is dissolved in ethanol at a mass ratio of 1:10, and then sterilized by membrane filtration to obtain a sterile solution containing Cordyceps militaris extract. The residual ethanol in the sterile solution containing Cordyceps militaris extract is ≤50ppm. Preferably, Cordyceps militaris extract is dissolved in a 30%-50% ethanol solution and sterilized by filtration through a 0.1μm Al2O3 ceramic membrane. Preferably, the Cordycepin content in the Cordyceps militaris extract is ≥0.8%, and the adenosine content is ≥0.3%.

[0015] In one embodiment, glucosamine hydrochloride is dissolved in pure water, the pH is adjusted to 4.8-5.2 using a sodium citrate buffer, and then sterilized by membrane filtration to obtain a sterile solution containing glucosamine hydrochloride. Preferably, glucosamine hydrochloride is dissolved in pure water at 30-50°C and sterilized by filtration through a 0.22μm PES membrane.

[0016] In one embodiment, calcium β-hydroxy-β-methylbutyrate is dissolved in pure water and sterilized by membrane filtration to obtain a sterile solution containing calcium β-hydroxy-β-methylbutyrate. Preferably, calcium β-hydroxy-β-methylbutyrate is dissolved in pure water at 40-65°C and sterilized by filtration through a 0.22μm PTFE membrane.

[0017] In one embodiment, the homogenization pressure is 180–220 bar; preferably, the homogenization temperature is 60–70°C.

[0018] In one embodiment, the sterilization temperature is 135–145°C; preferably, the sterilization time is 2–7 seconds.

[0019] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0020] (1) The modified milk provided by the present invention contains cordycepin in Cordyceps militaris, which enhances the biosynthesis and energy supply capacity of mitochondria by activating relevant signaling pathways, and provides an energy basis for GaHMB (β-hydroxy-β-methylbutyrate calcium) to function, thereby improving the synthesis efficiency of muscle protein; adenosine and glucosamine in Cordyceps militaris work synergistically to block inflammation-related signal transduction and reduce the decomposition of cartilage matrix; at the same time, glucosamine, as a glycosaminoglycan precursor, can significantly stimulate the synthesis efficiency of cartilage tissue and improve the utilization efficiency of sugar under the action of the signaling pathway regulated by adenosine, ultimately achieving a triple synergistic intervention on "muscle-joint-metabolism".

[0021] (2) The preparation method of the modified milk provided by the present invention adopts a membrane concentration-sterile ternary segmented addition process to achieve dual optimization of activity retention and stability. Membrane concentration is used to increase protein content to meet the high protein needs of middle-aged and elderly people. Secondly, in order to avoid the destruction of functional components by heat treatment, a sterile ternary segmented addition process is adopted. Compared with the traditional process, the GaHMB retention rate is increased by 18%. Detailed Implementation

[0022] The technical solutions in the embodiments of this disclosure will be clearly and completely described below. Obviously, the described embodiments are only some, not all, of the embodiments of this disclosure. Based on the embodiments of this disclosure, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this disclosure.

[0023] The terms "first" or "second," etc., used to designate numbers or ordinal numbers in this specification are for descriptive purposes only and should not be construed as indicating, explicitly or implicitly, relative importance or specifying the number of indicated technical features. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this specification, "a plurality of" means at least two, such as two, three, or more, unless otherwise explicitly specified.

[0024] The specific implementation methods of this disclosure will now be described in detail.

[0025] This application provides a high-protein modified milk rich in cordycepin, GaHMB (calcium β-hydroxy-β-methylbutyrate), and glucosamine prepared using aseptic post-addition technology, which has broad market prospects.

[0026] In one embodiment, this application provides a method for preparing a liquid modified milk, comprising homogenizing and sterilizing animal milk to obtain a sterilized base material; under aseptic conditions, adding a sterile solution containing glucosamine hydrochloride, a sterile solution containing calcium β-hydroxy-β-methylbutyrate, and a sterile solution containing Cordyceps militaris extract to the sterilized base material, wherein the Cordyceps militaris extract contains cordycepin and adenosine, and the liquid modified milk contains ≥1.6 mg / 100 mL of cordycepin and ≥0.6 mg / 100 mL of adenosine.

[0027] In this embodiment, the animal milk is first aseptically treated before the functional ingredients are added, achieving a dual optimization of the retention of the functional ingredients' activity and stability. Specifically, during dairy processing, especially in ultra-high temperature (UHT) sterilization, GaHMB is easily degraded by high temperatures, leading to the inactivation of heat-sensitive components. This method employs aseptic post-addition technology, avoiding the destruction of functional ingredients by subsequent sterilization heat treatment. Compared with traditional processes, the GaHMB retention rate is increased by 18%. Furthermore, GaHMB and glucosamine have poor solubility in neutral emulsions, and direct mixing easily leads to stratification. The method provided in this application adds the functional ingredients in solution form, effectively increasing the solubility of the functional ingredients and making the product solution homogeneous.

[0028] Furthermore, the modified milk provided in this application contains cordycepin from Cordyceps militaris, which enhances the biosynthesis and energy supply capacity of mitochondria by activating relevant signaling pathways, while providing an energy basis for GaHMB to function, thereby improving the synthesis efficiency of muscle protein; adenosine and glucosamine in Cordyceps militaris work synergistically to block inflammation-related signal transduction and reduce the decomposition of cartilage matrix; at the same time, glucosamine, as a glycosaminoglycan precursor, can significantly stimulate the synthesis efficiency of cartilage tissue and improve the utilization efficiency of sugar under the action of adenosine-regulated signaling pathways, ultimately achieving a triple synergistic intervention on "muscle-joint-metabolism".

[0029] In one embodiment, the method provided in this application includes RO membrane concentration of animal milk, after which the protein content is 5.8%-6.2%.

[0030] The animal milk can be fresh cow's milk. Preferably, the fresh cow's milk includes Jersey milk. The rare 1% Jersey fresh milk source from Huishan's own ranches can be used, as Jersey milk has higher nutrient levels than Holstein milk.

[0031] The raw milk is first concentrated using an RO membrane, and then further concentrated by nanofiltration to a protein content of 5.8%-6.2%, achieving a high protein content in the formulated milk. This invention's high-protein formulated milk, with a protein content of 5.8%-6.2%, can expand the market demand for high-protein products among middle-aged and elderly people.

[0032] In one embodiment, the method provided in this application includes homogenizing and sterilizing animal milk.

[0033] The homogenization process is carried out at a pressure of 180–220 bar (e.g., 180 bar, 190 bar, 200 bar, 210 bar, or 220 bar). The homogenization process is carried out at a temperature of 60–70°C (e.g., 60°C, 62°C, 64°C, 66°C, 68°C, or 70°C).

[0034] The sterilization temperature is 135–145°C (e.g., 135°C, 136°C, 138°C, 140°C, or 142°C). The sterilization time is 2–7 seconds (e.g., 2 seconds, 3 seconds, 4 seconds, 5 seconds, 6 seconds, or 7 seconds).

[0035] In one embodiment, the method provided in this application includes sequentially injecting an glucosamine hydrochloride solution, a calcium β-hydroxy-β-methylbutyrate solution, and a cordyceps militaris extract into a cooled sterilized substrate.

[0036] In one embodiment, based on parts by mass, with animal milk as 1000 parts, the amount of aseptic solution of glucosamine hydrochloride added is 0.5 to 3.0 parts, the amount of aseptic solution of GaHMB added is 0.5 to 3.0 parts, and the amount of aseptic solution of Cordyceps militaris extract added is 0.2 to 1.0 parts.

[0037] In this process, glucosamine hydrochloride is dissolved in pure water, the pH is adjusted to 4.8-5.2 using a sodium citrate buffer, and then sterilized by membrane filtration to obtain a sterile solution containing glucosamine hydrochloride. Preferably, glucosamine hydrochloride is dissolved in pure water at 30-50℃ and sterilized by filtration through a 0.22μm PES membrane.

[0038] GaHMB is dissolved in pure water and then filtered through a membrane to remove bacteria, resulting in a sterile solution containing GaHMB. Preferably, GaHMB is dissolved in pure water at 40-65℃ and then filtered through a 0.22μm PTFE membrane to remove bacteria.

[0039] Cordyceps militaris extract is dissolved in ethanol at a mass ratio of 1:10, and then sterilized by membrane filtration to obtain a sterile solution containing Cordyceps militaris extract. The residual ethanol in the sterile solution containing Cordyceps militaris extract is ≤50ppm. Preferably, Cordyceps militaris extract is dissolved in a 30%-50% ethanol solution and sterilized by filtration through a 0.1μm Al2O3 ceramic membrane. Preferably, the Cordycepin content in the Cordyceps militaris extract is ≥0.8%, and the adenosine content is ≥0.3%.

[0040] In some specific embodiments, the temperature at which the functional component is dissolved can be, for example, but not limited to, 30°C, 35°C, 40°C, 45°C, 50°C, 55°C, 60°C, or 65°C.

[0041] In some specific implementations, the product pH may be, for example, 4.8, 4.9, 5.0, 5.1, or 5.2.

[0042] In this embodiment, glucosamine hydrochloride solution, calcium β-hydroxy-β-methylbutyrate solution, and Cordyceps militaris extract solution are added sequentially at flow rates of 10–15 L / min, 8–12 L / min, and 5–8 L / min, respectively. This three-stage addition (i.e., sequential addition) avoids the combination of sulfate ions in glucosamine (glucosamine hydrochloride solution) with calcium ions to form GaSO4 precipitate, which would affect product stability. It also avoids the tendency of cordycepin to promote adenosine hydrolysis under acidic conditions, leading to the loss of active ingredients.

[0043] This invention employs a sterile, segmented addition process. Glucosamine hydrochloride, GaHMB aqueous solution, and Cordyceps militaris ethanol solution are dissolved in the base material sequentially under sterile conditions. Considering that in a milk system with pH > 6.5 (milk pH ≈ 6.7), the free calcium ions of GaHMB superimpose with the calcium in GaHMB itself, easily forming a precipitate, sodium citrate buffer is used to adjust the pH to 4.8–5.0, thus controlling the pH of the functional ingredient mixture to achieve optimal taste, flavor, and stability of the modified milk.

[0044] In one embodiment, the method provided in this application adds stabilizers and sweeteners to the animal milk before homogenizing and sterilizing it. Specifically, fresh milk, stabilizers, and sweeteners are mixed and then homogenized and sterilized.

[0045] Stabilizers prevent fat from rising to the top and proteins from sinking in the modified milk system, thus ensuring the stability of the modified milk.

[0046] The stabilizer includes two or more of the following: mono- and diglycerides of fatty acids, microcrystalline cellulose, sodium alginate, xanthan gum, and sodium carboxymethyl cellulose; preferably, the mass ratio of mono- and diglycerides of fatty acids, microcrystalline cellulose, sodium alginate, xanthan gum, and sodium carboxymethyl cellulose is (4.5-5.5):(1.5-3):(1.5-3):(0.5-2):(0.5-2).

[0047] In some specific embodiments, the mass ratio of mono- and diglyceride fatty acid esters, microcrystalline cellulose, sodium alginate, xanthan gum, and sodium carboxymethyl cellulose can be, for example, but not limited to, 4.5:1.5:1.5:1:1.5, 4.5:2:1.5:1:1, or 5.5:1.5:2:0.5:0.5.

[0048] The mass ratio of fresh milk to stabilizer is (850-900):(2-4). For example, the mass ratio of fresh milk to stabilizer can be, but is not limited to, 800:2, 820:2.3, 850:2.5, 900:3, or 950:4. Stabilizers composed in a certain mass ratio can improve the stability of modified milk. Specifically, high-protein modified milk, where free calcium ions easily form precipitates with GaHMB, often affects the stability and flavor of the modified milk. This invention overcomes the instability of modified milk by adding stabilizers, especially sodium alginate, which can chelate calcium ions and prevent precipitation.

[0049] The sweetener includes at least one of white sugar, sucralose, or acesulfame potassium; preferably, the mass ratio of fresh milk to sweetener is (850-950):(35-55) (e.g., 850:35, 850:40, 850:45, 900:50, 959:50, or 950:55).

[0050] In one embodiment, the functional ingredients can be aseptically mixed and aseptically filled after addition.

[0051] In the above embodiments, the preparation method of modified milk uses fresh milk as raw material, which is concentrated through membrane to increase the protein content. After homogenization and sterilization, it is cooled and then added in a segmented manner under aseptic conditions, along with a dissolved and mixed glucosamine hydrochloride solution, GaHMB, and Cordyceps militaris extract. By adding the ingredients under aseptic conditions, the heat loss of functional components is avoided, and the activity of GaHMB, glucosamine, and Cordyceps militaris is protected to the greatest extent.

[0052] In another aspect, the present invention provides a liquid modified milk comprising: animal milk, glucosamine hydrochloride, calcium β-hydroxy-β-methylbutyrate, and Cordyceps militaris extract, wherein the Cordyceps militaris extract contains cordycepin and adenosine, and the liquid modified milk contains ≥1.6 mg / 100 mL of cordycepin and ≥0.6 mg / 100 mL of adenosine.

[0053] The modified milk provided by this invention utilizes cordycepin from Cordyceps militaris to enhance mitochondrial biosynthesis and energy supply by activating relevant signaling pathways, while providing an energy basis for GaHMB to function, thereby improving the synthesis efficiency of muscle protein. Adenosine and glucosamine in Cordyceps militaris work synergistically to block inflammation-related signal transduction and reduce the breakdown of cartilage matrix. At the same time, glucosamine, as a glycosaminoglycan precursor, can significantly stimulate the synthesis efficiency of cartilage tissue and improve sugar utilization efficiency under the action of adenosine-regulated signaling pathways, ultimately achieving a triple synergistic intervention on "muscle-joint-metabolism".

[0054] In one embodiment, based on 1000 parts by weight of animal milk, the amount of glucosamine hydrochloride added is 0.5 to 3.0 parts, the amount of calcium β-hydroxy-β-methylbutyrate added is 0.5 to 3.0 parts, and the amount of Cordyceps militaris extract added is 0.2 to 1.0 parts.

[0055] In one embodiment, the protein content of the modified milk is 5.8%-6.2%.

[0056] The modified milk provided by this invention, through a synergistic "muscle-joint-metabolism" logic, can cover the middle-aged and elderly population. Utilizing aseptic post-addition technology, it enhances the retention rate of functional components, creating product value. Compared to solid supplements, which often result in lower compliance among the elderly due to swallowing difficulties, the high-protein liquid modified milk provided in this application, which effectively improves "sarcopenia-arthritis" in the elderly, can meet the growing market demand for functional liquid milk.

[0057] The embodiments of the present invention will now be described in detail with reference to examples and comparative examples.

[0058] Example 1

[0059] The modified milk in this embodiment is mainly made from the following raw materials by weight:

[0060] 850 parts Jersey raw milk with a protein content of 6.0% after membrane concentration, 3 parts stabilizer, 40 parts white sugar, and 0.1 parts sucralose.

[0061] The stabilizers include mono- and diglycerides of fatty acids, microcrystalline cellulose, sodium alginate, xanthan gum, and sodium carboxymethyl cellulose; the mass ratio of mono- and diglycerides of fatty acids, microcrystalline cellulose, sodium alginate, xanthan gum, and sodium carboxymethyl cellulose is 5:2:1.5:1:0.5.

[0062] The method for preparing the modified milk in this embodiment includes the following steps:

[0063] (1) Jersey raw milk acceptance: Jersey raw milk is released after passing the acceptance inspection according to the requirements for fresh raw milk.

[0064] (2) Filtration, cooling and storage: The filtration mesh size shall be in accordance with the Liquid Milk Process Specification; the storage shall be cooled to 0-4℃ and the temperature and time during storage shall be in accordance with the requirements of the Liquid Milk Process Specification.

[0065] (3) Milk purification: The qualified Jersey raw milk is purified, and the parameters are implemented in accordance with the requirements of the Liquid Milk Process Specification.

[0066] (4) Separation and concentration: If the physicochemical properties of the raw milk do not meet the design requirements, standardization (separation and concentration) treatment is required, and the process parameters shall be implemented in accordance with the requirements of the Liquid Milk Process Specification.

[0067] (5) Homogenization, pre-sterilization, cooling and storage: Jersey raw milk after homogenization is pre-sterilized and cooled to 2-6℃ for use. Homogenization pressure, pre-sterilization temperature and time parameters, and storage time shall be performed in accordance with the requirements of the Liquid Milk Process Specification.

[0068] (6) Ingredient 1: Preheat all or part of the ingredients to 73±1℃. Add the stabilizer and white sugar that are fully dry-mixed during material circulation. Circulate at this temperature for 20 minutes and cool to below 10℃ before use.

[0069] (7) Mixing 1: Put the cooled ingredients 1 and the remaining ingredients and milk into the mixing tank and stir for 20 to 30 minutes. The storage temperature of the liquid in the mixing tank should not be higher than 10°C.

[0070] (8) Homogenization: After passing the index inspection, homogenize at a pressure of 200 bar and a temperature of 65℃.

[0071] (9) Sterilization treatment: The homogenized mixture is sterilized at ultra-high temperature of 138℃ for 4s, and then cooled to 2-6℃ for storage.

[0072] (10) Ingredient 2: Dissolve glucosamine hydrochloride in pure water at 40°C, adjust the pH to 5.0 with sodium citrate, and filter through a 0.22μm PES membrane; dissolve GaHMB in pure water at 50°C and filter through a 0.22μm PTFE membrane; dissolve Cordyceps militaris extract in 40% ethanol and filter through a 0.1μm Al2O3 ceramic membrane, and store at 28°C in the dark.

[0073] (11) Mixing: Under sterile conditions, glucosamine hydrochloride solution, GaHMB solution and cordyceps militaris solution are added in stages to make the pH of the prepared milk 6.3-6.5; the amount of glucosamine hydrochloride solution added is 1 part, the amount of GaHMB added is 1 part, and the amount of cordyceps militaris extract added is 0.4 parts.

[0074] (12) Filling, aseptic addition, labeling, and printing date: After sterilizing the product, cool it and fill it.

[0075] (13) Finished product storage: After filling, the product is stored in a cold storage warehouse at 2-6℃.

[0076] Example 2

[0077] The modified milk in this embodiment is mainly made from the following raw materials by weight:

[0078] 850 parts Jersey raw milk with a protein content of 6.0% after membrane concentration, 3 parts stabilizer, 40 parts white sugar, and 0.1 parts sucralose.

[0079] The stabilizers include mono- and diglycerides of fatty acids, microcrystalline cellulose, sodium alginate, xanthan gum, and sodium carboxymethyl cellulose; the mass ratio of mono- and diglycerides of fatty acids, microcrystalline cellulose, sodium alginate, xanthan gum, and sodium carboxymethyl cellulose is 5:2:1.5:1:0.5.

[0080] The method for preparing the modified milk in this embodiment includes the following steps:

[0081] (1)~(10) Same as Example 1.

[0082] (11) Mixing: Under sterile conditions, glucosamine hydrochloride solution, GaHMB solution and cordyceps militaris solution are added in stages to make the pH of the prepared milk 6.3-6.5; the amount of glucosamine hydrochloride solution added is 0.8 parts, the amount of GaHMB added is 1 part, and the amount of cordyceps militaris extract added is 0.2 parts.

[0083] (12)-(13) Same as Example 1.

[0084] Example 3

[0085] The modified milk in this embodiment is mainly made from the following raw materials by weight:

[0086] 850 parts Jersey raw milk with a protein content of 6.0% after membrane concentration, 3 parts stabilizer, 40 parts white sugar, and 0.1 parts sucralose.

[0087] The stabilizers include mono- and diglycerides of fatty acids, microcrystalline cellulose, xanthan gum, and sodium carboxymethyl cellulose; the mass ratio of mono- and diglycerides of fatty acids, microcrystalline cellulose, xanthan gum, sodium carboxymethyl cellulose, and sucrose fatty acid esters is 5:2:1:0.5.

[0088] The method for preparing the modified milk in this embodiment includes the following steps:

[0089] (1)~(13) Same as Example 1.

[0090] Comparative Example 1

[0091] The modified milk raw material provided in this comparative example is the same as that in Example 1. The only difference between the preparation method and that in Example 1 is the addition of functional components using a traditional process, namely, the addition of amino acid glucose hydrochloride solution, GaHMB solution, and Cordyceps militaris extract in step 7) of the mixing process.

[0092] Comparative Example 2

[0093] The only difference between the modified milk raw material provided in this comparative example and that in Example 1 is that Cordyceps militaris extract is not added, and the preparation method is the same as in Example 1.

[0094] Comparative Example 3

[0095] The modified milk raw material provided in this comparative example is the same as that in Example 1. The only difference between the preparation method and that in Example 1 is that the functional ingredients are directly mixed and added, that is, in step 11), the amino acid glucose hydrochloride solution, GaHMB solution and Cordyceps militaris extract are aseptically mixed and then added to the base material.

[0096] Experimental Example

[0097] Experimental Example 1

[0098] Sensory evaluations were conducted on the modified milks of Examples 1-3 and Comparative Examples 1-3. The scoring criteria are detailed in Table 1, and the results are shown in Table 2.

[0099] Table 1 Sensory Quality Scoring Table for Modified Milk

[0100]

[0101] Table 2 Sensory Evaluation

[0102]

[0103] As can be seen from the data in Table 2, the modified milk preparation method provided by the present invention is reasonable and can reduce the unpleasant taste caused by Ga+ sedimentation rate compared with traditional processes.

[0104] Experiment Example 2

[0105] GaHMB retention rate (in HMB)

[0106] Table 3 GaHMB Retention Rate (90 days)

[0107]

[0108] As can be clearly seen from the data in Table 3, the aseptic ternary segmented addition process in the modified milk preparation method provided by this invention can significantly improve the retention rate of GaHMB compared to the traditional process, with an improvement of approximately 18.1%. At the same time, Cordyceps militaris extract also promotes the retention of GaHMB.

[0109] Experimental Example 3

[0110] Centrifugal sedimentation rate

[0111] Table 4 Centrifugal Sedimentation Rate

[0112]

[0113] As can be clearly seen from the data in Table 4, in the modified milk preparation method provided by this invention, the addition of sodium alginate can chelate calcium ions, thereby effectively reducing the formation of precipitation to a certain extent. Furthermore, the aseptic ternary segmented addition process is more effective in improving the precipitation problem compared to traditional processes.

[0114] While various embodiments of the invention have been shown and described herein, it will be apparent to those skilled in the art that such embodiments are provided by way of example only. Many modifications, alterations, and alternatives will occur to those skilled in the art without departing from the spirit and essence of the invention. It should be understood that various alternatives to the embodiments of the invention described herein may be employed in the practice of the invention. The appended claims are intended to define the scope of protection of the invention and therefore cover modular compositions, equivalents, or alternatives within the scope of these claims.

Claims

1. A liquid-based modified milk, characterized in that, include: Animal milk, glucosamine hydrochloride, calcium β-hydroxy-β-methylbutyrate, and Cordyceps militaris extract, wherein the Cordyceps militaris extract contains cordycepin and adenosine, and the liquid-prepared milk contains ≥1.6 mg / 100 mL of cordycepin and ≥0.6 mg / 100 mL of adenosine.

2. The liquid-based modified milk according to claim 1, characterized in that, Based on the mass fraction of 1000 parts of the animal milk, the amount of glucosamine hydrochloride added is 0.5 to 3.0 parts, the amount of calcium β-hydroxy-β-methylbutyrate added is 0.5 to 3.0 parts, and the amount of Cordyceps militaris extract added is 0.2 to 1.0 parts.

3. The liquid-based modified milk according to claim 2, characterized in that, The protein content of the modified milk is 5.8%-6.2%.

4. The liquid-based modified milk according to any one of claims 1-3, characterized in that, The modified milk also includes stabilizers, which include two or more of the following: mono- and diglycerides of fatty acids, microcrystalline cellulose, sodium alginate, xanthan gum, and sodium carboxymethyl cellulose. Preferably, the mass ratio of the animal milk to the stabilizer is (850-900):(2-4).

5. The liquid-based modified milk according to any one of claims 1-3, characterized in that, The modified milk also includes a sweetener, which includes at least one of white sugar, sucralose, or acesulfame potassium; Preferably, the mass ratio of the animal milk to the sweetener is (850-900):(35-55).

6. A method for preparing a liquid-modified emulsion as described in any one of claims 1-5, characterized in that, include: Animal milk is homogenized and sterilized to obtain sterilized base material; Under aseptic conditions, a sterile solution containing glucosamine hydrochloride, a sterile solution containing calcium β-hydroxy-β-methylbutyrate, and a sterile solution containing Cordyceps militaris extract are added to the sterilized base material. The Cordyceps militaris extract contains cordycepin and adenosine. The liquid-prepared milk contains ≥1.6 mg / 100 mL of cordycepin and ≥0.6 mg / 100 mL of adenosine.

7. The method for preparing liquid modified emulsion according to claim 6, characterized in that, Glucosamine hydrochloride solution, calcium β-hydroxy-β-methylbutyrate solution, and Cordyceps militaris extract solution were sequentially injected into the cooled sterilized substrate at flow rates of 10-15 L / min, 8-12 L / min, and 5-8 L / min, respectively. Preferably, based on 1000 parts by weight of the animal milk, the amount of glucosamine hydrochloride added is 0.5 to 3.0 parts, the amount of calcium β-hydroxy-β-methylbutyrate added is 0.5 to 3.0 parts, and the amount of Cordyceps militaris extract added is 0.2 to 1.0 parts.

8. The method for preparing liquid modified emulsion according to claim 6, characterized in that, The process preceding the homogenization and sterilization of the animal milk includes: Animal milk was concentrated using an RO membrane, and the protein content after membrane concentration was 5.8%-6.2%.

9. The method for preparing liquid modified emulsion according to claim 6, characterized in that, The glucosamine hydrochloride is dissolved in pure water, and the pH is adjusted to 4.8-5.2 with sodium citrate buffer. After sterilization by membrane filtration, a sterile solution containing glucosamine hydrochloride is obtained. Preferably, the glucosamine hydrochloride is dissolved in pure water at 30-50℃ and sterilized by filtration through a 0.22μm PES membrane. The calcium β-hydroxy-β-methylbutyrate is dissolved in pure water and sterilized by membrane filtration to obtain a sterile solution containing calcium β-hydroxy-β-methylbutyrate; preferably, the calcium β-hydroxy-β-methylbutyrate is dissolved in pure water at 40-65℃ and sterilized by filtration through a 0.22μm PTFE membrane. The Cordyceps militaris extract is dissolved in ethanol at a mass ratio of 1:10, and then sterilized by membrane filtration to obtain a sterile solution containing the Cordyceps militaris extract, wherein the residual ethanol in the sterile solution containing the Cordyceps militaris extract is ≤50ppm; preferably, the Cordyceps militaris extract is dissolved in a 30%-50% ethanol solution and sterilized by filtration through a 0.1μm Al2O3 ceramic membrane; preferably, the Cordycepin content in the Cordyceps militaris extract is ≥0.8%, and the adenosine content is ≥0.3%.

10. The method for preparing liquid modified emulsion according to claim 6, characterized in that, The homogenization process is carried out at a pressure of 180–220 bar; preferably, the homogenization process is carried out at a temperature of 60–70°C. The sterilization treatment temperature is 135-145℃; preferably, the sterilization treatment time is 2-7 seconds.

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