Low-temperature preservation and resuscitation device for reproductive medicine

Abstract

The invention discloses a low-temperature preservation and resuscitation device for reproductive medicine, and belongs to the technical field of low-temperature preservation and resuscitation. The resuscitation assembly is connected with the top of the base so as to resuscitate the sample test tube filled with the germ cells; the low-temperature preservation assembly is detachably arranged at the top of the resuscitation assembly and is communicated with the resuscitation assembly so as to perform low-temperature preservation on the sample test tube filled with the germ cells; wherein a switch assembly is arranged at the communicating position of the low-temperature preservation assembly and the recovery assembly, and opening and closing of the communicating position are achieved; and the driving assembly is connected to the low-temperature preservation assembly, so that the sample test tubes placed on the driving assembly are fed into the resuscitation assembly. Through integrated and automatic design, seamless transfer of the sample between preservation and recovery is realized, temperature fluctuation and pollution risks are reduced, the operation efficiency, the standardization degree and the cell survival rate are improved, and meanwhile, the cost is reduced.

Description

Technical Field

[0001] This invention belongs to the field of cryopreservation and resuscitation technology, specifically relating to a cryopreservation and resuscitation device for reproductive medicine. Background Technology

[0002] In the field of reproductive medicine, cryopreservation technology is widely used for the long-term preservation of sperm, eggs, and embryos.

[0003] Traditional methods typically involve storing samples individually in liquid nitrogen tanks, requiring manual transfer to a constant-temperature water bath during resuscitation. This traditional method has significant drawbacks: temperature fluctuations during manual transfer can easily lead to ice crystal formation, damaging cell structure and posing a high risk of sample viability loss; batch samples must be handled individually, which is time-consuming, labor-intensive, and difficult to standardize, resulting in low operational efficiency; and the separation of preservation and resuscitation equipment increases the risk of contamination and equipment costs.

[0004] Therefore, there is an urgent need for an integrated and automated cryopreservation and resuscitation device to ensure sample viability and improve operational reliability. Summary of the Invention

[0005] To achieve the above objectives, the present invention provides a cryopreservation and resuscitation device for reproductive medicine, which adopts the following technical solution: A cryopreservation and resuscitation device for reproductive medicine, comprising: Base; A resuscitation assembly, which is connected to the top of the base, for resuscitating a sample tube containing germ cells; A cryopreservation component is detachably mounted on top of the resuscitation component and communicates with the resuscitation component to cryopreserve sample tubes containing germ cells; wherein, a switch component is provided at the communication point between the cryopreservation component and the resuscitation component to realize the opening and closing of the communication point; At least one drive component is connected to the cryopreservation component to deliver a sample tube placed on the drive component into the resuscitation component.

[0006] Furthermore, the resuscitation assembly includes a resuscitation box, a vibrating element, a vibrating support plate, and multiple vibrating sleeves; the resuscitation box is connected to the top of the base; the vibrating support plate is disposed inside the resuscitation box and connected to the bottom of the resuscitation box by a spring; the vibrating element is disposed on the vibrating support plate to vibrate the vibrating support plate; the vibrating support plate is annular and has multiple first openings; the vibrating sleeves are inserted into the first openings, and the multiple vibrating sleeves are arranged one-to-one with the multiple first openings.

[0007] Furthermore, the resuscitation assembly also includes a drive unit, a stirring shaft, and a heating plate; the stirring shaft is rotatably disposed inside the resuscitation chamber and located at the center of the vibration support plate; the drive unit is disposed inside the base and is correspondingly disposed to the stirring shaft; the drive end of the drive unit passes through the resuscitation chamber and is connected to the stirring shaft to drive the stirring shaft to rotate; multiple heating plates are provided, and an array of multiple heating plates is disposed on the stirring shaft and connected to the stirring shaft respectively to heat the water in the resuscitation chamber.

[0008] Furthermore, the cryogenic preservation assembly includes a preservation box, a controller, and multiple placement plates; a liquid nitrogen layer is provided on the inner wall of the preservation box, and the liquid nitrogen layer is hollow inside to fill the sample tubes inside the preservation box for cooling; multiple partitions are provided inside the liquid nitrogen layer to divide the liquid nitrogen layer into multiple sub-spaces; sliding grooves are provided on the partitions; multiple second openings are provided on the outer wall of the preservation box, and the multiple second openings correspond one-to-one with the multiple sliding grooves, and the second openings communicate with the sliding grooves; Multiple drive components are provided, and each drive component is corresponding to one of the multiple second openings, and the drive components are connected to the outer wall of the storage box. The top of the storage box is provided with multiple third openings, which communicate with the interior of the storage box and are correspondingly arranged with multiple vibration sleeves; the bottom of the storage box is provided with multiple fourth openings, which communicate with the interior of the resuscitation box; the multiple third openings, multiple fourth openings, multiple partitions and multiple placement plates are arranged in a corresponding manner. The controller is connected to the preservation box; the first end of the placement plate is provided with a placement hole for placing the sample tube; an electromagnet is connected to the placement hole, and the electromagnet is electrically connected to the controller to attract the iron ring at the top of the sample tube; the first end of the placement plate is located inside the preservation box, and the second end passes through the sliding groove and the second opening and is connected to the driving assembly. The driving assembly drives the placement plate to slide up and down in the sliding groove, so that the sample tube passes through the fourth opening and is sent into the resuscitation box.

[0009] Furthermore, the top of the storage box is connected to multiple liquid nitrogen tubes, and each of the multiple liquid nitrogen tubes is arranged in a one-to-one correspondence with a multiple of the subspaces; one end of each liquid nitrogen tube is connected to the subspace, and the other end is connected to a liquid nitrogen filling device to fill the subspace with liquid nitrogen.

[0010] Furthermore, an insulation layer is provided between the inner wall of the storage box and the liquid nitrogen layer.

[0011] Furthermore, an electric switch door is provided at the fourth opening to control the opening and closing of the fourth opening.

[0012] Furthermore, the drive assembly includes a drive motor, a lead screw, a mounting plate, and a movable plate; the mounting plate is fixedly connected to the outer wall of the storage box; the lead screw is connected to the bottom of the mounting plate; the fixed end of the drive motor is connected to the top of the mounting plate, and the driving end passes through the mounting plate and is connected to the lead screw; one end of the movable plate is connected to the placement plate, and the other end is connected to the lead screw, and the movable plate is driven by the drive motor to slide up and down along the lead screw.

[0013] Beneficial effects: The present invention provides a cryopreservation and resuscitation device for reproductive medicine, in which the preservation and resuscitation functions are integrated into the same device. The cryopreservation component and the resuscitation component are directly connected. The sample tubes are seamlessly transferred for preservation and resuscitation through the drive component, without exposure to the external environment, effectively avoiding secondary ice crystal formation and significantly improving cell survival rate. The linkage of the drive component, controller, electromagnet, and electric door can realize the automatic transfer of multiple samples in a single batch without human intervention, shortening the resuscitation operation time. Moreover, the operation process is completely standardized, reducing the dependence on personnel experience. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the overall structure of the cryopreservation and resuscitation device for reproductive medicine of the present invention; Figure 2 This is a schematic diagram of the internal structure of the cryopreservation and resuscitation device for reproductive medicine of the present invention; Figure 3 This is a cross-sectional view of the cryopreservation component of the cryopreservation and resuscitation device for reproductive medicine of the present invention; Figure 4 This is a cross-sectional view of the resuscitation component of the cryopreservation and resuscitation device for reproductive medicine of the present invention; Figure 5 This is a schematic diagram of the connection between the sample tube and the placement plate in the low-temperature preservation and resuscitation device for reproductive medicine of the present invention. The components are as follows: 1. Base; 2. Spring; 3. Vibration receiving plate; 4. Vibration sleeve; 5. Resuscitation box; 6. Fourth opening; 7. Lead screw; 8. Moving plate; 9. Electric opening and closing door; 10. Mounting plate; 11. Drive motor; 12. Insulation layer; 13. Liquid nitrogen layer; 14. Sample tube; 15. Heating plate; 16. Stirring shaft; 17. Drive unit; 18. Electromagnet; 19. Placement hole; 20. Placement plate; 21. Insulation cover; 22. Partition; 23. Storage box. Detailed Implementation

[0015] The above description is merely an overview of the technical solution of the present invention. In order to better understand the technical means of the present invention and to implement it in accordance with the contents of the specification, and to make the above and other objects, features and advantages of the present invention more apparent and understandable, specific embodiments of the present invention are described below.

[0016] Example 1

[0017] Reference Figures 1 to 5 A cryopreservation and resuscitation device for reproductive medicine, comprising: Base 1; A resuscitation assembly is connected to the top of the base 1 to resuscitate the sample tube 14 containing germ cells; The cryopreservation component is detachably mounted on top of the resuscitation component and communicates with the resuscitation component to cryopreserve the sample tube 14 containing germ cells; wherein, a switch component is provided at the connection between the cryopreservation component and the resuscitation component to realize the opening and closing of the connection. At least one drive component is connected to the cryopreservation component so that the sample tube 14 placed on the drive component is delivered into the resuscitation component.

[0018] The above technical solution, with its detachable design for both the cryogenic preservation and recovery components, facilitates equipment cleaning and component replacement, extending service life.

[0019] In this embodiment, the resuscitation assembly includes a resuscitation box 5, a vibrating element (not shown in the figure), a vibrating support plate 3, and multiple vibrating sleeves 4; the resuscitation box 5 is connected to the top of the base 1; the vibrating support plate 3 is disposed inside the resuscitation box 5 and connected to the bottom of the resuscitation box 5 by a spring 2; the vibrating element is disposed on the vibrating support plate 3 to vibrate the support plate 3; the vibrating support plate 3 is annular and has multiple first openings; the vibrating sleeves 4 are inserted into the first openings, and the multiple vibrating sleeves 4 are arranged one-to-one with the multiple first openings.

[0020] In this embodiment, the resuscitation assembly further includes a drive unit 17, a stirring shaft 16, and a heating plate 15. The stirring shaft 16 is rotatably disposed inside the resuscitation chamber 5 and located at the center of the vibration receiving plate 3. The drive unit 17 is disposed inside the base 1 and is correspondingly disposed to the stirring shaft 16. The drive end of the drive unit 17 passes through the resuscitation chamber 5 and is connected to the stirring shaft 16 to drive the stirring shaft 16 to rotate. Multiple heating plates 15 are disposed in an array on the stirring shaft 16 and are respectively connected to the stirring shaft 16 to heat the water in the resuscitation chamber 5.

[0021] With the above technical solution, the sample tube 14 is inserted into the vibrating sleeve 4, and the vibrating component drives the vibrating support plate 3 through the spring 2, so that the sample tube 14 shakes evenly in the water; the heating plate 15 cooperates with the stirring shaft 16 to achieve precise control of water temperature, which is better than traditional water baths, and ensures a stable and consistent resuscitation environment.

[0022] In this embodiment, the cryogenic preservation assembly includes a preservation box 23, a controller, and multiple placement plates 20; a liquid nitrogen layer 13 is provided on the inner wall of the preservation box 23, and the liquid nitrogen layer 13 is hollow inside to fill the sample tubes 14 inside the preservation box 23 for cooling; multiple partitions 22 are provided inside the liquid nitrogen layer 13 to divide the liquid nitrogen layer 13 into multiple sub-spaces; sliding grooves are provided on the partitions 22; multiple second openings are provided on the outer wall of the preservation box 23, and the multiple second openings are provided one-to-one with the multiple sliding grooves, and the second openings are connected to the sliding grooves; There are multiple drive components, and each drive component is set to correspond one-to-one with a multiple second opening. The drive components are connected to the outer wall of the storage box 23. The top of the storage box 23 is provided with multiple third openings, which communicate with the interior of the storage box 23 and are respectively provided with multiple vibration sleeves 4; the bottom of the storage box 23 is provided with multiple fourth openings 6, which communicate with the interior of the resuscitation box 5; the multiple third openings, multiple fourth openings 6, multiple partitions 22 and multiple placement plates 20 are respectively provided. The controller is connected to the storage box 23; the first end of the placement plate 20 is provided with a placement hole 19 for placing the sample tube 14; an electromagnet 18 is connected to the placement hole 19, and the electromagnet 18 is electrically connected to the controller to attract the iron ring at the top of the sample tube 14; the first end of the placement plate 20 is placed inside the storage box 23, and the second end passes through the sliding groove and the second opening and is connected to the drive assembly. The drive assembly drives the placement plate 20 to slide up and down in the sliding groove, so that the sample tube 14 passes through the fourth opening 6 and is sent into the resuscitation box 5.

[0023] An insulation cover 21 is installed on the third opening.

[0024] Through the above technical solution, the iron ring is fixedly sleeved on the top of the sample tube, and the electromagnet 18 adsorbs the iron ring on the sample tube 14. Combined with the vertical drive path of the screw drive, zero mechanical collision transfer is achieved, reducing the sample tube breakage rate and ensuring that the sample tube enters the resuscitation box in a vertical posture, avoiding solution spillage.

[0025] In this embodiment, the top of the storage box 23 is connected to multiple liquid nitrogen tubes, and the multiple liquid nitrogen tubes are arranged one-to-one with multiple subspaces; one end of the liquid nitrogen tube is connected to the subspace, and the other end is connected to a liquid nitrogen filling device to fill the subspace with liquid nitrogen.

[0026] Through the above technical solution, the liquid nitrogen layer 13 of the cryogenic preservation component is divided into multiple sub-spaces by the partition 22, and the amount of liquid nitrogen in each sub-space can be controlled independently to adapt to the preservation needs of different samples. At the same time, multiple placement plates 20 are set one-to-one with multiple vibration sleeves 4, so that the sample tube 14 in the placement hole 19 passes through the fourth opening 6 and enters the vibration sleeve 4 in the resuscitation box 5.

[0027] In this embodiment, an insulation layer 12 is provided between the inner wall of the storage box 23 and the liquid nitrogen layer 13.

[0028] Through the above technical solution, the insulation layer 12 can maintain the temperature inside the storage box 23, reduce the amount of liquid nitrogen evaporation, and reduce the frequency of liquid nitrogen replenishment.

[0029] In this embodiment, an electric switch door 9 is provided at the fourth opening 6. The electric switch door 9 is electrically connected to the controller to control the opening and closing of the fourth opening 6.

[0030] In this embodiment, the drive assembly includes a drive motor 11, a lead screw 7, a mounting plate 10, and a movable plate 8; the mounting plate 10 is fixedly connected to the outer wall of the storage box 23; the lead screw 7 is connected to the bottom of the mounting plate 10; the fixed end of the drive motor 11 is connected to the top of the mounting plate 10, and the drive end passes through the mounting plate 10 and is connected to the lead screw 7; one end of the movable plate 8 is connected to the placement plate 20, and the other end is connected to the lead screw 7, and the movable plate 8 is driven by the drive motor 11 to slide up and down along the lead screw 7.

[0031] Among them, the drive motor 11 and the vibrating component are electrically connected to the controller, and the amplitude of the vibrating component is adjusted by the controller.

[0032] The working method of the cryopreservation and resuscitation device for reproductive medicine provided by this invention is as follows: S1. Equipment Inspection: Confirm that the base 1 is placed stably and that the resuscitation component and the cryogenic preservation component are tightly connected; check whether the electric switch door 9, drive component and controller are powered on normally; fill with liquid nitrogen, fill each subspace of liquid nitrogen layer 13 through the liquid nitrogen pipe at the top of the preservation box 23 (set the filling amount according to the sample type, and monitor the liquid nitrogen level by the controller) to ensure stable preservation temperature; inject an appropriate amount of distilled water into the resuscitation box 5 (the water level should cover the bottom of the vibration sleeve 4), set the resuscitation temperature through the controller, start the heating plate 15 and stirring shaft 16 to preheat, and wait for the water temperature to stabilize before use.

[0033] S2. Sample Placement: Open the third opening at the top of the preservation box 23 and insert the sample tube 14 containing germ cells (with a pre-set iron ring at the top) into the placement hole 19 of the placement plate 20; the controller activates the electromagnet 18, which magnetically attracts the iron ring at the top of the test tube to ensure that the test tube is firmly fixed; close the third opening, and the controller controls the electric switch door 9 to close the fourth opening 6, so that the preservation box 23 forms a closed environment; the liquid nitrogen layer 13 maintains the low temperature through continuous evaporation, so as to achieve long-term stable preservation of the sample.

[0034] S3. Start Resuscitation: Select the drive component corresponding to the sample to be resuscitated through the controller and set the resuscitation time; the controller starts the drive component, the drive motor 11 drives the lead screw 7 to rotate, the moving plate 8 moves down along the lead screw 7, and pushes the placement plate 20 to move downward through the sliding groove; at the same time, the electric switch door 9 at the corresponding position opens, the sample tube 14 passes through the fourth opening 6 and enters the resuscitation box 5, and is inserted into the vibration sleeve 4; the vibration component is started, and the vibration receiving plate 3 drives the vibration sleeve 4 to vibrate slightly, so that the solution in the sample tube 14 is heated evenly; the heating plate 15 continues to heat, and the stirring shaft 16 drives the heating plate 15 to rotate, ensuring that the water temperature in the resuscitation box 5 is uniform.

[0035] S4. Resuscitation Completed and Returned to Position: After the resuscitation time is over, the controller drives the placement plate 20 to move upward, the sample tube 14 is removed from the vibration sleeve 4, passes through the fourth opening 6 and returns to the storage box 23; the electric switch door 9 is closed, the electromagnet 18 is restarted to fix the test tube, and the resuscitation process is completed.

[0036] S5. After resuscitation is completed, turn off the controller power, clean the water in the resuscitation box 5, and disinfect the vibration sleeve 4, placement plate 20 and other components; replenish liquid nitrogen to liquid nitrogen layer 13 to ensure that the preservation components maintain a low temperature environment; check the status of each component to prepare for the next operation.

[0037] Through the coordinated action of drive components, electromagnet 18, controller, etc., the fully automated operation of sample tubes from liquid nitrogen preservation, precise transfer, and constant temperature recovery is achieved, solving the problems of temperature fluctuation and operation error caused by manual intervention and improving sample survival rate.

[0038] The above are merely preferred embodiments of the present invention and do not constitute any limitation on the technical scope of the present invention. Therefore, any minor modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present invention shall still fall within the scope of the technical solution of the present invention.

Claims

1. A device for low temperature preservation and recovery in reproductive medicine, characterized in that, The utility model relates to a sample test tube cryopreservation and recovery device, which comprises a base, a recovery assembly connected to the top of the base to recover a sample test tube containing reproductive cells, a low-temperature preservation assembly detachably arranged on the top of the recovery assembly and in communication with the recovery assembly to cryopreserve the sample test tube containing reproductive cells, wherein the low-temperature preservation assembly is provided with a switch assembly at the communication position with the recovery assembly to realize the opening and closing of the communication position, and at least one driving assembly connected to the low-temperature preservation assembly to send the sample test tube placed on the driving assembly into the recovery assembly. The recovery assembly comprises a recovery box, a vibrating member, a vibrating receiving plate and a plurality of vibrating sleeves; the recovery box is connected to the top of the base; the vibrating receiving plate is arranged in the recovery box and connected to the bottom of the recovery box through a spring; the vibrating member is arranged on the vibrating receiving plate to vibrate the vibrating receiving plate; the vibrating receiving plate is in the shape of a circular ring and provided with a plurality of first holes; the vibrating sleeves are inserted into the first holes, and the plurality of vibrating sleeves are arranged in one-to-one correspondence with the plurality of first holes. The recovery assembly further comprises a driving part, a stirring shaft and a plurality of heating plates; the stirring shaft is rotatably arranged in the recovery box and located at the center position of the vibrating receiving plate; the driving part is arranged in the base and arranged in correspondence with the stirring shaft; the driving end of the driving part penetrates through the recovery box and is connected with the stirring shaft to drive the stirring shaft to rotate; the plurality of heating plates are arranged in an array on the stirring shaft and connected with the stirring shaft respectively to heat the water in the recovery box. The low-temperature preservation assembly comprises a preservation box, a controller and a plurality of placing plates; the inner wall of the preservation box is provided with a liquid nitrogen layer, which is hollow inside to fill liquid nitrogen to cool the sample test tube in the preservation box; a plurality of partitions are arranged inside the liquid nitrogen layer to divide the liquid nitrogen layer into a plurality of subspaces; the partitions are provided with sliding grooves; the outer wall of the preservation box is provided with a plurality of second holes, which are arranged in one-to-one correspondence with the plurality of sliding grooves and in communication with the sliding grooves; The driving assembly is provided with a plurality of driving assemblies, which are arranged in one-to-one correspondence with the plurality of second holes and connected with the outer wall of the preservation box; 2. The device for cryopreservation and recovery in reproductive medicine according to claim 1, characterized in that, The top of the preservation box is provided with a plurality of third holes, which are in communication with the inside of the preservation box and arranged in one-to-one correspondence with the plurality of vibrating sleeves; the bottom of the preservation box is provided with a plurality of fourth holes to make the inside of the preservation box in communication with the inside of the recovery box; the plurality of third holes, the plurality of fourth holes, the plurality of partitions and the plurality of placing plates are arranged in one-to-one correspondence.

3. The device for cryopreservation and recovery in reproductive medicine according to claim 2, characterized in that, ​ 4. The device for cryopreservation and recovery in reproductive medicine according to claim 2, characterized in that, ​ ​ ​ The controller is connected to the preservation box; the first end of the placing plate is provided with a placing hole for placing a sample test tube; an electromagnet is connected to the placing hole and electrically connected to the controller to attract an iron ring on the top of the sample test tube; the first end of the placing plate is arranged in the preservation box, and the second end is connected to the driving assembly through the sliding groove and the second opening; the sample test tube is sent into the recovery box through the fourth opening by driving the placing plate to slide up and down in the sliding groove by the driving assembly.

5. The device for cryopreservation and recovery in reproductive medicine according to claim 4, characterized in that, A plurality of liquid nitrogen pipes are connected to the top of the preservation box, and the plurality of liquid nitrogen pipes are arranged one-to-one with the plurality of subspaces; one end of the liquid nitrogen pipe is in communication with the subspace, and the other end is externally connected to a liquid nitrogen filling device to fill liquid nitrogen into the subspace.

6. The device for cryopreservation and recovery in reproductive medicine according to claim 4, characterized in that, A heat preservation layer is arranged between the inner wall of the preservation box and the liquid nitrogen layer.

7. The device for cryopreservation and recovery in reproductive medicine according to claim 4, characterized in that, An electric switch door is arranged at the fourth opening to control the opening and closing of the fourth opening.

8. The device for cryopreservation and recovery in reproductive medicine according to claim 4, characterized in that, The driving assembly comprises a driving motor, a lead screw, a mounting plate and a moving plate; the mounting plate is fixedly connected to the outer wall of the preservation box; the lead screw is connected to the bottom of the mounting plate; the fixed end of the driving motor is connected to the top of the mounting plate, and the driving end is connected to the lead screw through the mounting plate; one end of the moving plate is connected to the placing plate, and the other end is connected to the lead screw; the moving plate is driven to slide up and down along the lead screw by the driving motor.

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