Automatic incubator for microbial stem cells

Abstract

The invention relates to the technical field of cell culture boxes, in particular to an automatic microbial stem cell culture box which comprises a culture box body, an annular box body is fixedly installed in an inner cavity of the culture box body, an inner container is fixedly installed in an inner cavity of the annular box body, and a motor is fixedly installed on the back of the annular box body. A first connecting rod is fixedly mounted at the output end of the motor, and a first mounting block is welded to the side, away from the motor, of the first connecting rod. According to the device, condensate water generated in the previous heating stage is recycled and converted into fine fog drops through an atomization mechanism, and the fine fog drops are evenly sprayed again to cover the surface of the outer wall of the inner container; in the process, a continuous and stable liquid heat buffer layer is formed, and the layer can effectively absorb and disperse residual heat of the retained hot steam and prevent the residual heat from being continuously transmitted to the interior of the inner container, so that the phenomenon of temperature overshoot is remarkably inhibited, and the accuracy of overall temperature control and the stability of the system are improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cell incubators, in particular to an automatic microbial stem cell incubator. BACKGROUND

[0002] The hot steam microbial stem cell incubator is a special incubation device specially adapted to the culture needs of microbial stem cells. Its core design logic is to use the latent heat released during the condensation of hot steam as the main heating source. Through a closed-loop system of steam generation, pipeline transportation, condensation heat release in the interlayer, and condensate water recovery, a constant temperature culture environment is provided for microbial stem cells. However, due to the physical properties and heating mechanism of hot steam, the existing hot steam microbial stem cell incubator has significant technical bottlenecks in the accuracy and response speed of temperature regulation. The core problem lies in the fact that the process of latent heat release during steam condensation belongs to a "passive heat release" mode. When the temperature in the cabin approaches or reaches the set value, even if the steam input is turned off, the residual steam in the pipeline and interlayer will continue to condense and release heat, causing temperature "overshoot". Since microbial stem cells have very low tolerance to temperature fluctuations, the overshoot problem of temperature regulation will have an adverse effect on the culture effect. This easily triggers cell cycle disorder or abnormal activation of differentiation-related signal pathways in microbial stem cells, which to some extent restricts the application reliability of the hot steam microbial stem cell incubator in high-precision stem cell culture scenarios. Therefore, the present application provides an automatic microbial stem cell incubator. SUMMARY

[0003] The present application aims to provide an automatic microbial stem cell incubator to solve the problems raised in the background art.

[0004] The technical solution of the present application is an automatic microbial stem cell incubator, which comprises a culture box main body, an annular box body fixedly installed in the inner cavity of the culture box main body, an inner container fixedly installed in the inner cavity of the annular box body, a motor fixedly installed on the back of the annular box body, and a connecting rod one fixedly installed on the output end of the motor. The side of the connecting rod one away from the motor is welded with a mounting block one, and the side of the mounting block one away from the connecting rod one is welded with a sliding rod one. The sliding rod one is slidably arranged with a mounting block two on the rod body. The annular box body inner cavity is provided with a scraper, and the two ends of the scraper are welded with two symmetrical limiting rods. One of the limiting rods is welded with the side wall of the mounting block two.

[0005] Preferably, a spring is wound around the slide rod, and the spring is connected between mounting block one and mounting block two. A limiting frame one is welded to one side wall of the connecting rod, and the limiting rod is slidably disposed within the limiting frame one. Two symmetrical limiting frames two are welded to the front and rear side walls of the annular box cavity. Because the "layer" surrounds and wraps the inner liner, the heat in the high-temperature steam can be quickly and efficiently transferred to the inner liner, thereby causing the temperature in the inner liner to rise rapidly to the working temperature. At the same time, the motor drives the connecting rod one, mounting block one, slide rod one, mounting block two, limiting rod and scraper to rotate. The limiting rod is doubly limited by the limiting frame one and the limiting frame two, so that the limiting rod can drive the scraper to slide along the outer wall of the "rounded square" inner liner, thereby scraping off the condensate on the outer wall of the "rounded square" inner liner and preventing it from affecting the heat transfer of the high-temperature steam. In addition, the rotating water tank can agitate the hot steam in the "layer" to make it more evenly distributed.

[0006] Preferably, a water tank is welded to the top of the scraper, and several atomizing nozzles are evenly spaced at the bottom of the water tank. A water inlet is provided on the side wall of the water tank, and several sliding rods are slidably arranged on the side wall of the water tank. Each sliding rod has a top plate welded to its top, and a spring is wound around the body of each sliding rod. Several springs are respectively connected between the water tank and the corresponding top plate. A partition is welded between several sliding rods. Since low-temperature condensate accumulates at the bottom of the annular box cavity, when the water tank follows the scraper "skimming" over the bottom of the annular box cavity, the partition moves into the water tank under the impact of the condensate and opens the water inlet, thereby allowing the moving water tank to automatically "scoop up" some of the condensate.

[0007] Preferably, two symmetrical mounting plates are welded to the side walls at both ends of the water tank. Each mounting plate has a sliding rod three slidably mounted on its top. Each sliding rod three has a base welded to its side away from the corresponding mounting plate. Each base has a swing rod rotatably connected to its top. Each swing rod has a return spring connected to its side wall. Several return springs are respectively connected to the top of the corresponding base on their sides away from the corresponding swing rod. Each base has an abutment block welded to its top.

[0008] Preferably, each of the three sliding rods is welded to a piston plate at its bottom, and two symmetrical sleeves are welded to the side walls at both ends of the water tank. Several piston plates are slidably arranged in the inner cavities of the corresponding sleeves. Each sleeve is connected to an air pipe at its bottom, and the side of the air pipe away from the corresponding sleeve is connected to the side wall of the water tank. Each piston plate is provided with a circulating air hole. When the swing rod, which follows the water tank in a "reverse" motion, "passes" over the teeth on the inner circular surface of the inner toothed ring, the swing rod moves up and down under the combined action of the teeth and the elastic force of the three springs. The swing rod drives the base, the three sliding rods, and the piston plates to move up and down, thereby continuously "pressing" the gas in the sleeves into the water tank. This causes the cooler condensate "scooped" from inside the water tank to be sprayed out through the atomizing nozzle, thus forming a "liquid heat buffer layer" on the outer wall of the "rounded square" inner liner. This prevents the hot steam that has not been discharged in time from the "interlayer" between the annular box and the inner liner from continuing to transfer heat into the inner liner, thereby improving the accuracy of the "temperature control" of this device.

[0009] Preferably, two symmetrical internal toothed rings are welded to the inner wall of the annular box.

[0010] Preferably, an air inlet is provided on the side wall of the annular box, an air outlet is provided on the top of the annular box, and a liquid filling pipe is connected to the side wall of the annular box.

[0011] This invention provides an automated microbial stem cell culture box with the following improvements and advantages compared to the prior art: During use, this device effectively scrapes away condensate adhering to the outer wall of the rounded square inner liner, significantly improving the efficiency of heat transfer from hot steam to the inner liner. Furthermore, when the inner liner temperature reaches a preset value, the device actively scrapes away the still-warm condensate accumulated on the outer wall and further recycles the condensate generated during the previous heating stage, converting it into fine droplets through an atomization mechanism and re-spraying it evenly onto the outer surface of the inner liner. This process forms a continuous and stable liquid heat buffer layer, which effectively absorbs and disperses the residual heat from the retained steam, preventing it from continuously transferring into the inner liner. This significantly suppresses temperature overshoot and improves the overall accuracy of temperature control and system stability. Attached Figure Description

[0012] The present invention will be further explained below with reference to the accompanying drawings and embodiments: Figure 1 This is a three-dimensional structural schematic diagram of the present invention; Figure 2 This is a schematic diagram of the annular box structure of the present invention; Figure 3 This is a schematic diagram of the inner liner structure of the present invention; Figure 4This is a schematic diagram of the water tank structure of the present invention; Figure 5 This is a schematic diagram of the scraper structure of the present invention; Figure 6 This is the present invention. Figure 4 Enlarged schematic diagram of part A; Figure 7 This is a schematic diagram of the internal toothed ring structure of the present invention; Figure 8 This is the present invention. Figure 7 Enlarged schematic diagram of section B structure; Figure 9 This is a schematic diagram of the inner cavity structure of the sleeve of the present invention; Figure 10 This is the present invention. Figure 7 An enlarged schematic diagram of the C-section structure.

[0013] Explanation of reference numerals in the attached figures: 1. Incubator body; 2. Annular chamber; 3. Inner liner; 4. Motor; 5. Connecting rod one; 6. Mounting block one; 7. Slide rod one; 8. Mounting block two; 9. Scraper; 10. Limiting rod; 11. Spring one; 12. Limiting frame one; 13. Water tank; 14. Water inlet; 15. Slide rod two; 16. Top plate; 17. Spring two; 18. Partition plate; 19. Mounting plate; 20. Slide rod three; 21. Base; 22. Swing rod; 23. Return spring; 24. Abutment block; 25. Atomizing nozzle; 26. Piston plate; 27. Sleeve; 28. Air pipe; 29. ​​Circulating air hole; 30. Liquid filling pipe; 31. Internal toothed ring; 32. Air inlet; 33. Air outlet; 34. Limiting frame two; 35. Spring three. Detailed Implementation

[0014] The present invention will now be described in detail, and the technical solutions in the embodiments of the present invention will be clearly and completely described. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0015] This invention provides an improved automated microbial stem cell culture box. The technical solution of this invention is as follows: like Figures 1-10As shown, an automated microbial stem cell culture box includes a culture box body 1, an annular box 2 fixedly installed inside the culture box body 1, an air inlet 32 ​​on the side wall of the annular box 2, an air outlet 33 on the top of the annular box 2, a liquid addition pipe 30 connected to the side wall of the annular box 2, an inner liner 3 fixedly installed inside the annular box 2, a motor 4 fixedly installed on the back of the annular box 2, and the output end of the motor 4 extending into the annular box 2. A connecting rod 5 is fixedly installed on the output end of the motor 4, and a mounting block is welded to the side of the connecting rod 5 away from the motor 4. Mounting block 16 has a sliding rod 7 welded to the side of mounting block 16 away from connecting rod 15. Mounting block 28 is slidably mounted on the sliding rod 7. A scraper 9 is installed inside the annular box 2. Two symmetrical limiting rods 10 are welded to the side walls of both ends of the scraper 9, and one of the limiting rods 10 is welded to the side wall of mounting block 28. A spring 11 is wound around the sliding rod and is connected between mounting block 16 and mounting block 28. A limiting frame 12 is welded to the side wall of connecting rod 15, and the limiting rod 10 is slidably mounted inside the limiting frame 12. The front of the annular box 2 is... Two symmetrical limiting frames 34 are welded to the rear two side walls. In use, the culture dish is placed on the shelf in the inner liner 3, and then the main body 1 of the incubator is closed. Then the steam generator inside the main body 1 of the incubator operates, and the high-temperature steam generated enters the "sandwich" between the annular box 2 and the inner liner 3 through the air inlet 32. Since the "sandwich" surrounds the inner liner 3, the heat in the high-temperature steam can be transferred to the inner liner 3 quickly and efficiently, thereby causing the temperature in the inner liner 3 to rise rapidly to the working temperature. At the same time, the motor 4 drives the connecting rod. 5. Mounting block 1, 6. Sliding rod 1, 7. Mounting block 2, 8. Limiting rod 10 and scraper 9 rotate. The limiting rod 10 is doubly limited by limiting frame 12 and limiting frame 2 34, so that the limiting rod 10 can drive the scraper 9 to slide along the outer wall of the "rounded square" inner tank 3. The scraper 9 then scrapes away the condensate on the outer wall of the "rounded square" inner tank 3, preventing it from affecting the heat transfer of the high-temperature steam. In addition, the rotating water tank 13 can agitate the hot steam in the "jacket" to make it more evenly distributed.

[0016] Furthermore, a water tank 13 is welded to the top of the scraper 9, and several atomizing nozzles 25 are evenly spaced at the bottom of the water tank 13. A water inlet 14 is opened on the side wall of the water tank 13, and several sliding rods 15 are slidably arranged on the side wall of the water tank 13. A top plate 16 is welded to the top of each sliding rod 15, and a spring 17 is wound around the rod of each sliding rod 15. Several springs 17 are respectively connected between the water tank 13 and the corresponding top plate 16. A partition 18 is welded between several sliding rods 15. Since the bottom of the inner cavity of the annular box 2 is filled with condensed water at a low temperature, when the water tank 13 follows the scraper 9 to "skim" the bottom of the inner cavity of the annular box 2, the partition 18 moves into the water tank 13 under the impact of the condensed water and opens the water inlet 14, thereby allowing the moving water tank 13 to automatically "scoop" some of the condensed water.

[0017] Furthermore, two symmetrical mounting plates 19 are welded to the side walls at both ends of the water tank 13. Each mounting plate 19 has a sliding rod 20 slidably mounted on its top. A base 21 is welded to the side of each sliding rod 20 away from the corresponding mounting plate 19. A rocker arm 22 is rotatably connected to the top of each base 21. A return spring 23 is connected to the side wall of each rocker arm 22, and several return springs 23 are connected to the top of their respective bases 21 on their sides away from the corresponding rocker arm 22. An abutment block 24 is welded to the top of each base 21. A piston plate 26 is welded to the bottom of each sliding rod 20. Two symmetrical sleeves 27 are welded to the side walls at both ends of the water tank 13, and several piston plates 26 are slidably mounted inside the corresponding sleeves 27. An air pipe 28 is connected to the bottom of each sleeve 27, and the side of several air pipes 28 away from the corresponding sleeve is connected to the side wall of the water tank 13. Each piston plate 26 has a circulating air hole 29. Two symmetrical internal toothed rings 31 are welded on the inner wall of the annular box 2. When the swing rod 22, which follows the water tank 13 to "reverse", "skips" the teeth on the inner circular surface of the internal toothed ring 31, the swing rod 22 moves up and down under the combined action of the teeth and the elastic force of the spring 35. The swing rod 22 drives the base 21, the slide rod 30 and the piston plate 26 to move up and down, thereby continuously "pressing" the gas in the sleeve 27 into the water tank 13. This causes the cooler condensate "scooped" from inside the water tank 13 to be sprayed out through the atomizing nozzle 25, thereby forming a "liquid heat buffer layer" on the outer wall of the "rounded square" inner liner 3. This prevents the hot steam that has not been discharged in time from the "interlayer" between the annular box 2 and the inner liner 3 from continuing to transfer heat to the inner liner 3, thereby improving the accuracy of the "temperature control" of this device.

[0018] Working Principle: During use, place the petri dish on the shelf inside the inner liner 3, then close the main body 1 of the incubator. The steam generator inside the main body 1 then operates, generating high-temperature steam that enters the "sandwich" between the annular chamber 2 and the inner liner 3 through the air inlet 32. Because the "sandwich" surrounds the inner liner 3, the heat from the high-temperature steam can be quickly and efficiently transferred to the inner liner 3, causing the temperature inside the inner liner 3 to rise rapidly to the working temperature. Simultaneously, the motor 4 drives the connecting rod 5, mounting block 6, sliding rod 7, mounting block 8, limiting rod 10, and scraper 9 to rotate. The limiting rod 10 is doubly limited by limiting frame 12 and limiting frame 34, allowing the limiting rod 10 to drive the scraper 9 to slide along the outer wall of the "rounded square" inner liner 3, thereby... The scraper 9 scrapes away the condensate on the outer wall of the rounded square inner liner 3 to prevent it from affecting the heat transfer of the high-temperature steam. In addition, the rotating water tank 13 can agitate the hot steam in the "jacket" to make it more evenly distributed. The scraped condensate drips down the outer wall of the inner liner 3 to the bottom of the inner cavity of the annular box 2 and accumulates there. It should be noted that the bottom of the annular box 2 is provided with a drain hole. After the culture is completed, the valve at the drain hole is opened to drain the water. During the above process, because the condensate at a lower temperature accumulates at the bottom of the inner cavity of the annular box 2, when the water tank 13 follows the scraper 9 "skimming" over the bottom of the inner cavity of the annular box 2, the partition 18 moves into the water tank 13 under the impact of the condensate and opens the inlet 14, so that the moving water tank 13 automatically "scoops up" some of the condensate. Once the temperature inside the inner liner 3 reaches the operating temperature, the steam generator in the incubator body 1 stops operating. Then, the motor 4 drives the scraper 9 to rotate in the opposite direction. The moving scraper 9 removes the residual heat condensate from the outer wall of the rounded square inner liner 3, preventing it from transferring heat further into the inner liner 3. Simultaneously, the rotating scraper 9 drives the water tank 13, mounting plate 19, slide bar 30, base 21, and swing rod 22 to rotate. When the swing rod 22, following the water tank 13 in the opposite direction, passes over the teeth on the inner surface of the inner toothed ring 31, it reciprocates up and down under the combined action of the teeth and the spring 35. This reciprocating motion drives the base 21, slide bar 30, and piston plate 26, thereby continuously pushing the gas in the sleeve 27... The water is "pressed" into the water tank 13, causing the cooler condensate "scooped" from inside the water tank 13 to be sprayed out through the atomizing nozzle 25. This forms a "liquid heat buffer layer" on the outer wall of the "rounded square" inner liner 3, thus preventing the hot steam that has not been discharged in time from the "interlayer" between the annular box 2 and the inner liner 3 from continuing to transfer heat to the inner liner 3, thereby improving the accuracy of the "temperature control" of this device. It should be noted that when the motor 4 drives the swing arm 22 to "rotate forward", the swing arm 22 will compress the return spring 23 when it is pushed by the teeth. At this time, the piston plate 26 will not move up and down. Similarly, if it is necessary to clean the scale that may exist on the wall of the inner liner 3, cleaning fluid is added to the inside of the annular box 2 through the liquid filling pipe 30, and then the above steps are repeated to achieve the effect of atomizing the cleaning fluid and scraping the structure.

[0019] The foregoing description enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. An automated microbial stem cell culture chamber, comprising a main body (1), characterized in that: An annular box (2) is fixedly installed in the inner cavity of the incubator body (1). An inner liner (3) is fixedly installed in the inner cavity of the annular box (2). A motor (4) is fixedly installed on the back of the annular box (2), and the output end of the motor (4) extends into the inner cavity of the annular box (2). A connecting rod (5) is fixedly installed at the output end of the motor (4). An installation block (6) is welded to the side of the connecting rod (5) away from the motor (4). A sliding rod (7) is welded to the side of the installation block (6) away from the connecting rod (5). An installation block (8) is slidably arranged on the sliding rod (7). A scraper (9) is provided in the inner cavity of the annular box (2). Two symmetrical limiting rods (10) are welded to the side walls of both ends of the scraper (9), and one of the limiting rods (10) is welded to the side wall of the installation block (8).

2. The automated microbial stem cell culture box according to claim 1, characterized in that: A spring (11) is wound around the slide bar, and the spring is connected between the mounting block (6) and the mounting block (8). A limit frame (12) is welded on the side wall of the connecting rod (5), and the limit rod (10) is slidably disposed in the limit frame (12). Two symmetrical limit frames (34) are welded on the side walls of the front and rear ends of the inner cavity of the annular box (2).

3. The automated microbial stem cell culture box according to claim 1, characterized in that: A water tank (13) is welded to the top of the scraper (9). Several atomizing nozzles (25) are evenly spaced at the bottom of the water tank (13). A water inlet (14) is opened on the side wall of the water tank (13). Several sliding rods (15) are slidably arranged on the side wall of the water tank (13). A top plate (16) is welded to the top of each sliding rod (15). A spring (17) is wound around the rod of each sliding rod (15). Several springs (17) are respectively connected between the water tank (13) and the corresponding top plate (16). A partition (18) is welded between several sliding rods (15).

4. The automated microbial stem cell culture box according to claim 3, characterized in that: Two symmetrical mounting plates (19) are welded to the side walls at both ends of the water tank (13). Each mounting plate (19) has a sliding rod (20) slidably mounted on its top. Each sliding rod (20) has a base (21) welded to the side away from the corresponding mounting plate (19). Each sliding rod (20) has a spring (35) wound around its body. Several springs (35) are connected between the corresponding mounting plate (19) and the corresponding base (21). Each base (21) has a swing rod (22) rotatably connected to its top. Each swing rod (22) has a return spring (23) connected to its side wall. Several return springs (23) are connected to the top of the corresponding base (21) on the side away from the corresponding swing rod (22). Each base (21) has an abutment block (24) welded to its top.

5. The automated microbial stem cell culture box according to claim 4, characterized in that: Each of the three slide rods (20) has a piston plate (26) welded to its bottom. Two symmetrical sleeves (27) are welded to the side walls of both ends of the water tank (13). Several piston plates (26) are slidably arranged in the inner cavity of the corresponding sleeves (27). Each sleeve (27) has an air pipe (28) connected to its bottom. The side of several air pipes (28) away from the corresponding sleeve (27) is connected to the side wall of the water tank (13). Each piston plate (26) has a circulation air hole (29).

6. The automated microbial stem cell culture box according to claim 1, characterized in that: Two symmetrical internal toothed rings (31) are welded to the inner wall of the annular box (2).

7. The automated microbial stem cell culture box according to claim 1, characterized in that: An air inlet (32) is provided on the side wall of the annular box (2), an air outlet (33) is provided on the top of the annular box (2), and a liquid filling pipe (30) is connected to the side wall of the annular box (2).

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