Nucleic acid or protein high-throughput detection equipment based on bottom scanning

Abstract

The invention discloses nucleic acid or protein high-throughput detection equipment based on bottom scanning, which comprises a detection pool assembly used for bearing a nucleic acid or protein sample to be detected, and the detection pool assembly can accommodate a plurality of detection units to realize high-throughput detection; the optical detection module is arranged below the detection pool assembly and is used for carrying out optical detection on a sample in the detection pool assembly from the bottom; the optical detection module comprises a light source, a semi-reflecting and semi-transmitting mirror, a filter group, a photosensitive element and a light source detection component.

Description

Technical Field

[0001] This invention relates to the technical field of a bottom-scanning-based high-throughput detection device for nucleic acids or proteins, and more particularly to a bottom-scanning-based high-throughput detection device for nucleic acids or proteins. Background Technology

[0002] In molecular biology research and clinical testing, the quantitative analysis of nucleic acids (such as DNA and RNA) and proteins is a fundamental and crucial task. Currently, nucleic acid or protein detection devices commonly used in the market, such as Thermo's Qubit detection device, have been applied to a certain extent in scientific research and clinical fields.

[0003] However, the existing Thermo Qubit detection equipment and its supporting equipment have obvious limitations in actual use: on the one hand, the operation process is relatively cumbersome, requiring multiple manual sample transfers and equipment debugging steps, which not only increases the workload of operators, but also easily affects the accuracy of the detection results due to human error; on the other hand, its detection throughput is low, and the number of samples that can be processed in one test is limited, which is difficult to meet the needs of large-scale sample detection. The problem of low efficiency is particularly prominent when facing scenarios such as high-throughput screening and batch sample analysis. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of existing Thermo Qubit detection devices, such as cumbersome operation and inability to achieve high-throughput detection, and to provide a high-throughput detection device for nucleic acids or proteins based on bottom scanning, so as to simplify the operation process, improve detection efficiency, and meet the needs of large-scale sample detection.

[0005] To address the aforementioned technical problems, one of the objectives of this application is achieved through the following technical solution: A high-throughput nucleic acid or protein detection device based on bottom scanning, comprising: A detection pool assembly is used to hold nucleic acid or protein samples to be detected. The detection pool assembly can accommodate multiple detection units to achieve high-throughput detection. An optical detection module is disposed below the detection pool assembly and is used to perform optical detection on the sample in the detection pool assembly from the bottom; the optical detection module includes a light source, a semi-reflective mirror, a filter group, a photosensitive element, and a light source detection component; A scanning drive mechanism is connected to the optical detection module or the detection pool assembly and is used to drive the optical detection module to move relative to the detection pool assembly, or to drive the detection pool assembly to move relative to the optical detection module, so as to realize the sequential scanning and detection of multiple detection units in the detection pool assembly. The data processing and control unit is electrically connected to the optical detection module and the scanning drive mechanism, respectively. It is used to control the opening and closing of the light source, the movement of the scanning drive mechanism, and to receive the light signal transmitted by the photosensitive element and perform data processing to obtain the detection results of nucleic acid or protein samples.

[0006] Furthermore, the optical detection module can be configured as a single-tube dual-wavelength detection mode or a single-wavelength detection mode; when configured as a single-wavelength detection mode, a single-wavelength detection module of different wavelengths can be selected according to the detection requirements, such as a 470nm single-wavelength detection module or a 630nm single-wavelength detection module.

[0007] Furthermore, the 470nm single-wavelength detection module includes a 470nm light source, filters of 426-495nm and 510-580nm, a semi-reflective mirror, a photosensitive element, and light source detection components; the 630nm single-wavelength detection module includes a 630nm light source, filters of ≤650nm and 665-725nm, a semi-reflective mirror, a photosensitive element, and light source detection components.

[0008] Furthermore, when multi-wavelength detection is required, the optical detection module can be composed of multiple single-wavelength detection modules of different wavelengths stacked together to meet the diverse wavelength requirements of different detection projects.

[0009] Furthermore, the detection unit in the detection pool assembly corresponds to the optical path of the optical detection module, ensuring that the light emitted by the light source can accurately illuminate the sample and that the light signal after passing through the sample can be effectively received by the photosensitive element.

[0010] Compared with the prior art, the present invention has the following beneficial effects: A high-throughput nucleic acid or protein detection device based on bottom scanning is disclosed, relating to the field of biodetection technology. The device includes a detection cell assembly, an optical detection module, a scanning drive mechanism, and a data processing and control unit. The detection cell assembly can accommodate multiple detection units to achieve high-throughput detection; the optical detection module is located below the detection cell assembly and employs a combination of single-wavelength detection modules to achieve single-tube dual-wavelength, single-wavelength, or multi-wavelength detection; the scanning drive mechanism drives the optical detection module to move relative to the detection cell assembly, achieving bottom scanning; the data processing and control unit controls the device operation and processes the detection data. This invention is simple to operate, enables high-throughput detection, and offers high flexibility and accuracy, meeting the needs of large-scale nucleic acid or protein sample detection.

[0011] Simple to operate: This invention adopts a bottom-scanning detection method, which eliminates the need for complex sample transfer and positioning operations, simplifies the detection process, and reduces the workload of operators and the impact of human error.

[0012] High-throughput detection: The detection pool assembly can accommodate multiple detection units. Combined with the scanning drive mechanism, it can perform rapid sequential detection on multiple samples, greatly improving detection efficiency and meeting the needs of large-scale sample detection.

[0013] High detection flexibility: The optical detection module can be configured as a single-tube dual-wavelength detection mode, a single-wavelength detection mode, or a multi-wavelength detection mode as needed, which can adapt to the different wavelength requirements of different nucleic acid or protein detection projects and expand the application range of the equipment.

[0014] High detection accuracy: By rationally designing the optical path of the optical detection module and the structure of the detection cell components, stable transmission and effective reception of optical signals are ensured, thereby improving the accuracy and reliability of the detection results. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the present application; Figure 2 This is a schematic diagram of the present application; Figure 3 This is a schematic diagram of the present application; Figure 4 This is a schematic diagram of the present application; Figure 5 This is a schematic diagram of this application. Detailed Implementation

[0016] The present application will now be further described in conjunction with the accompanying drawings and specific embodiments. It should be noted that, without conflict, the various embodiments or technical features described below can be arbitrarily combined to form new embodiments.

[0017] In the description of this application, it should be understood that the terms "upper", "lower", "left", "right", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application.

[0018] The terms "first," "second," etc., used in this application are used to distinguish similar objects and not to describe a specific order or sequence. It should be understood that such use of data can be interchanged where appropriate so that embodiments of this application can be implemented in orders other than those illustrated or described herein, and the objects distinguished by "first," "second," etc., are generally of the same class and the number of objects is not limited; for example, a first object can be one or more. Furthermore, in the specification and claims, "and / or" indicates at least one of the connected objects, and the character " / " generally indicates an "or" relationship between the preceding and following objects as a bottom-scanning-based high-throughput nucleic acid or protein detection device. Example 1:

[0019] A high-throughput detection device for nucleic acids or proteins based on bottom scanning, such as Figures 1-5 As shown, it includes: A detection pool assembly is used to hold nucleic acid or protein samples to be detected. The detection pool assembly can accommodate multiple detection units to achieve high-throughput detection. An optical detection module is disposed below the detection pool assembly and is used to perform optical detection on the sample in the detection pool assembly from the bottom; the optical detection module includes a light source, a semi-reflective mirror, a filter group, a photosensitive element, and a light source detection component; A scanning drive mechanism is connected to the optical detection module or the detection pool assembly and is used to drive the optical detection module to move relative to the detection pool assembly, or to drive the detection pool assembly to move relative to the optical detection module, so as to realize the sequential scanning and detection of multiple detection units in the detection pool assembly. The data processing and control unit is electrically connected to the optical detection module and the scanning drive mechanism, respectively. It is used to control the opening and closing of the light source, the movement of the scanning drive mechanism, and to receive the light signal transmitted by the photosensitive element and perform data processing to obtain the detection results of nucleic acid or protein samples.

[0020] Furthermore, the optical detection module can be configured as a single-tube dual-wavelength detection mode or a single-wavelength detection mode; when configured as a single-wavelength detection mode, a single-wavelength detection module of different wavelengths can be selected according to the detection requirements, such as a 470nm single-wavelength detection module or a 630nm single-wavelength detection module.

[0021] Furthermore, the 470nm single-wavelength detection module includes a 470nm light source, filters of 426-495nm and 510-580nm, a semi-reflective mirror, a photosensitive element, and light source detection components; the 630nm single-wavelength detection module includes a 630nm light source, filters of ≤650nm and 665-725nm, a semi-reflective mirror, a photosensitive element, and light source detection components.

[0022] Furthermore, when multi-wavelength detection is required, the optical detection module can be composed of multiple single-wavelength detection modules of different wavelengths stacked together to meet the diverse wavelength requirements of different detection projects.

[0023] Furthermore, the detection unit in the detection pool assembly corresponds to the optical path of the optical detection module, ensuring that the light emitted by the light source can accurately illuminate the sample and that the light signal after passing through the sample can be effectively received by the photosensitive element.

[0024] A high-throughput nucleic acid or protein detection device based on bottom scanning includes a detection pool assembly, an optical detection module, a scanning drive mechanism, and a data processing and control unit.

[0025] The detection pool assembly adopts a 96-well plate structure, with each well serving as a detection unit. It can accommodate nucleic acid or protein samples to be tested and can simultaneously carry 96 samples for detection, achieving high-throughput detection.

[0026] The optical detection module is located below the 96-well plate and consists of multiple stacked single-wavelength detection modules, including a 470nm single-wavelength detection module and a 630nm single-wavelength detection module. The 470nm single-wavelength detection module includes a 470nm light source, filters of 426-495nm and 510-580nm, a semi-reflective mirror, photosensitive element 1, and light source detection components. The 630nm single-wavelength detection module includes a 630nm light source, filters of ≤650nm and 665-725nm, a semi-reflective mirror, photosensitive element 2, and light source detection components.

[0027] The scanning drive mechanism adopts a motor-driven linear guide structure, which is connected to the optical detection module. It can drive the optical detection module to move along the length and width of the 96-well plate, thereby realizing the sequential scanning and detection of each detection unit in the 96-well plate.

[0028] The data processing and control unit uses an industrial computer, which is electrically connected to the light source, light source detection components, photosensitive elements, and the motor of the scanning drive mechanism in the optical detection module via data cables. It can control the on / off switching of the 470nm and 630nm light sources, control the operation of the motor of the scanning drive mechanism to move the optical detection module below the designated detection unit; simultaneously, it receives the light signals transmitted by photosensitive elements 1 and 2, processes and analyzes the light signals, calculates the detection results such as the concentration of nucleic acid or protein samples, and displays the results on the computer screen or stores them.

[0029] The working process of this device is as follows: Add the nucleic acid or protein samples to be tested into the respective detection units of the 96-well plate; Place the 96-well plate with the loaded sample into the detection position, ensuring that the relative positions of the detection cell assembly and the optical detection module are accurate; The detection parameters can be set through the data processing and control unit, such as selecting the detection wavelength (470nm, 630nm or both), scanning speed, etc. The detection program is started, and the data processing and control unit controls the scanning drive mechanism to move the optical detection module, while simultaneously controlling the corresponding light source to turn on. When the optical detection module moves under a certain detection unit, the light emitted by the light source is reflected by the semi-reflective lens and then shines on the sample through the bottom of the detection cell assembly. After passing through the sample, the light signal passes through the semi-reflective lens, is filtered by the corresponding filter, and is received by the photosensitive element. The photosensitive element converts the light signal into an electrical signal and transmits it to the data processing and control unit. The light source detection component is used to monitor the output intensity of the light source to ensure the stable operation of the light source. The data processing and control unit processes and analyzes the received electrical signals to obtain the detection results of the samples within the detection unit; Driven by the scanning drive mechanism, the optical inspection module sequentially scans and inspects all inspection units within the 96-well plate. The data processing and control unit summarizes and processes all inspection results, and finally outputs a complete inspection report.

[0030] The above embodiments are merely preferred embodiments of this application and should not be construed as limiting the scope of protection of this application. Any non-substantial changes and substitutions made by those skilled in the art based on this application shall fall within the scope of protection claimed by this application.

Claims

1. A high-throughput nucleic acid or protein detection device based on bottom scanning, characterized in that: include A detection pool assembly is used to hold nucleic acid or protein samples to be detected. The detection pool assembly can accommodate multiple detection units to achieve high-throughput detection. An optical detection module is disposed below the detection pool assembly and is used to perform optical detection on the sample in the detection pool assembly from the bottom; the optical detection module includes a light source, a semi-reflective mirror, a filter group, a photosensitive element, and a light source detection component; A scanning drive mechanism is connected to the optical detection module or the detection pool assembly and is used to drive the optical detection module to move relative to the detection pool assembly, or to drive the detection pool assembly to move relative to the optical detection module, so as to realize the sequential scanning and detection of multiple detection units in the detection pool assembly. The data processing and control unit is electrically connected to the optical detection module and the scanning drive mechanism, respectively. It is used to control the opening and closing of the light source, the movement of the scanning drive mechanism, and to receive the light signal transmitted by the photosensitive element and perform data processing to obtain the detection results of nucleic acid or protein samples.

2. The high-throughput nucleic acid or protein detection device based on bottom scanning according to claim 1, characterized in that: The optical detection module can be configured as a single-tube dual-wavelength detection mode or a single-wavelength detection mode. When configured as a single-wavelength detection mode, a single-wavelength detection module of different wavelengths can be selected according to the detection requirements, such as a 470nm single-wavelength detection module or a 630nm single-wavelength detection module.

3. The high-throughput nucleic acid or protein detection device based on bottom scanning according to claim 2, characterized in that: The 470nm single-wavelength detection module includes a 470nm light source, filters of 426-495nm and 510-580nm, a semi-reflective mirror, a photosensitive element, and light source detection components; the 630nm single-wavelength detection module includes a 630nm light source, filters of ≤650nm and 665-725nm, a semi-reflective mirror, a photosensitive element, and light source detection components.

4. The high-throughput nucleic acid or protein detection device based on bottom scanning according to claim 3, characterized in that: When multi-wavelength detection is required, the optical detection module can be composed of multiple single-wavelength detection modules with different wavelengths stacked together to meet the diverse wavelength requirements of different detection projects.

5. A high-throughput nucleic acid or protein detection device based on bottom scanning according to claim 4, characterized in that: The detection unit in the detection pool assembly corresponds to the optical path of the optical detection module, ensuring that the light emitted by the light source can accurately illuminate the sample and that the light signal after passing through the sample can be effectively received by the photosensitive element.

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