Microfluidic cartridge

Abstract

The application belongs to the technical field of micro-fluidic chip, and particularly relates to a micro-fluidic card box; the micro-fluidic card box comprises a first solution storage part, a piercing part and a second solution storage part; the first solution storage part is internally provided with a first limiting part and a fifth chamber; the top surface of the piercing part is a second limiting part, a third limiting part is arranged below the second limiting part, a first groove is formed between the second limiting part and the third limiting part, the piercing part is internally provided with a first channel, a second channel and a third channel which are in communication with each other, the third channel is in communication with the outside, a second groove is arranged above the third channel, a fourth limiting part and a solid spike part of a needle are arranged below the third channel, a third groove is formed between the fourth limiting part and the third channel; on the second solution storage part, a sixth chamber and a seventh chamber are in communication through a sixth channel, a sixth chamber and an eighth chamber are in communication through a fifth channel, and the inner diameter of the fifth channel is smaller than the diameter of the top surface of the piercing part; the application enables the solution to flow to a target chamber in sequence through centrifugation, and the solution is not contaminated during the transfer process.

Description

A microfluidic cartridge Technical Field

[0001] This invention relates to the field of microfluidic chip technology, and more particularly to a microfluidic card cartridge. Background Technology

[0002] Microfluidics is a multidisciplinary technology aimed at precisely manipulating and processing fluids at a microscale to achieve specific scientific research and engineering applications. In the biomedical field, microfluidics integrates biochemical reactions onto a single chip, controlling liquid flow to accomplish specific functions. However, current mainstream microfluidic technologies typically require external piping and multiple pumps and valves to drive the directional flow of solutions. This leads to complex chip structures and system designs, and the external piping and pumps are prone to contamination, resulting in unreliable experimental results or even experimental failure. Furthermore, the chips are costly, difficult to manufacture, and require high-quality materials. Therefore, designing a simple, easy-to-manufacture, and contamination-resistant microfluidic cartridge is a pressing technical challenge.

[0003] Existing mainstream microfluidic technologies typically require external piping and multiple pumps and valves to drive the directional flow of solutions. This results in complex chip structures and system designs. Furthermore, the external piping and pumps and valves are prone to leaving dead volumes after repeated use, causing contamination and leading to unreliable or even erroneous experimental results. Summary of the Invention

[0004] The purpose of this invention is to provide a simple-to-operate and contamination-preventing microfluidic cartridge to solve the technical problems of complex chip structure design and contamination in existing technologies. This invention simplifies the process of sequentially and directionally releasing the solution within the chip by rationally designing the microfluidic cartridge structure and using centrifugal force to drive the directional flow of liquid. This facilitates control and replaces the traditional complex and cumbersome piping design and pump / valve start-stop procedures. It also avoids contamination caused by solution residue in pumps, valves, and piping during transfer, ensuring the accuracy of experimental results.

[0005] This invention provides a microfluidic cartridge, comprising a first solution storage element, a puncture element, and a second solution storage element; wherein:

[0006] The first solution storage device includes a fifth chamber, and a first limiting part is provided on the top of the fifth chamber;

[0007] The top surface of the piercing part extends outward to form a second limiting part, the top surface of the second limiting part abuts against the bottom surface of the first limiting part, a third limiting part is provided below the second limiting part, a first groove is formed between the second limiting part and the third limiting part, the second limiting part and the third limiting part are movably disposed in the fifth chamber through the sealing ring in the first groove; the top surface of the second limiting part has an opening, the opening extends downward to form a first channel, the first channel is connected to the second channel and the third channel in sequence, the third channel is connected to the outside, the bottom of the third channel is a solid spike, the solid spike includes a fourth limiting part and a needle, the part between the fourth limiting part and the third channel forms a third groove, and a sealing ring is provided in the third groove;

[0008] The second solution storage unit has a sixth chamber at the top and a seventh and eighth chamber at the bottom. The eighth chamber is located directly below the fifth chamber, and the sixth and eighth chambers are connected by a fifth channel.

[0009] During operation, the third channel of the puncture device is located in and connected to the sixth chamber. The needle tip falls entirely into the eighth chamber. The third groove is located in the fifth channel. The sealing ring in the third groove is compressed by the inner wall of the fifth channel, which isolates the eighth chamber from the outside. The sixth and seventh chambers are connected through the sixth channel. Under the action of centrifugal force, the solution flows sequentially through the first, second, and third channels and then enters the seventh chamber through the sixth channel, achieving directional flow.

[0010] In this invention, a second groove is provided above the third channel, and a sealing ring is provided inside the second groove.

[0011] In this invention, the first solution storage device further includes a fourth chamber, the bottom of which is connected to the top of the fifth chamber;

[0012] During operation, the drive rod is placed in the fourth chamber, and external force pushes the drive rod to move the puncture piece downward until the third limiting part of the puncture piece is located in the fifth chamber, the needle is located in the eighth chamber, and the second groove just slides into the fifth channel. The sealing ring in the second groove is squeezed by the inner wall of the fifth channel. Under the action of centrifugal force, the solution flows through the first channel, the second channel, and the third channel in sequence before entering the eighth chamber, realizing directional flow.

[0013] In this invention, the inner diameter of the fifth channel is smaller than the diameter of the third limiting part of the puncture member, a sealing member is provided at the bottom of the eighth chamber, and a fourth channel is provided on the needle tip.

[0014] The present invention also includes a reaction element, which is fixedly disposed below the second solution storage element. A seventh channel is provided on the top of the reaction element, which is located directly below the eighth chamber and is connected to the lower chamber of the reaction element.

[0015] During operation, the drive rod is placed into the fourth chamber, and external force pushes the drive rod to move the piercing part downward until the third limiting part is stuck above the fifth channel. The needle pierces the seal at the bottom of the eighth chamber. The fourth channel is connected to the eighth chamber and the seventh channel. Under the action of centrifugal force, the solution flows through the first channel, the second channel, the third channel, the eighth chamber, and the seventh channel in sequence before entering the lower chamber of the reaction vessel.

[0016] In this invention, the two ends of the second solution storage device are respectively fitted onto the bottom of the first solution storage device and the top of the reaction device, and the second solution storage device is connected to the first solution storage device and the reaction device by snap-fit.

[0017] In this invention, the first solution storage device further includes a first chamber, a second chamber, and a third chamber. The bottom of the first chamber is connected to the bottom of the fourth chamber and the top of the fifth chamber. The bottom of the second chamber is connected to the bottom of the third chamber. The bottom of the third chamber is connected to the bottom of the fourth chamber. The bottom of the second chamber is higher than the bottom of the third chamber. The bottom of the second chamber is not connected to the bottom of the first chamber or the bottom of the fourth chamber. The bottom of the first chamber is not connected to the bottom of the third chamber. The height of the solution after being injected into the first chamber is lower than the bottom of the third chamber.

[0018] In this invention, the lower part of the reaction vessel is provided with a tenth chamber and an eleventh chamber. The tenth chamber and the eleventh chamber are interconnected through a ninth channel. The eighth channel is used to discharge bubbles from the solution in the eleventh chamber. The seventh channel is connected to the tenth chamber.

[0019] In this invention, the first chamber is used to add the lysed sample solution, the second chamber is equipped with a washing solution sac, the third chamber is equipped with an elution solution sac, the first channel is equipped with a nucleic acid adsorbent for adsorbing nucleic acids, and the eighth chamber is pre-stored with a reaction solution.

[0020] In this invention, the inner diameter of the first channel is larger than the inner diameter of the second channel.

[0021] Compared with the prior art, the beneficial effects of the present invention are as follows: By rationally designing the structure of the microfluidic cartridge, the present invention enables the solution in the chamber to flow sequentially and directionally to the target chamber during centrifugation, and the solution does not cross-contaminate during the transfer process. Under the action of centrifugal force, trace amounts of solution can be transferred to the next chamber without residue, avoiding trace residue and ensuring the accuracy of experimental results. Attached Figure Description

[0022] Figure 1 is a schematic diagram of the microfluidic card.

[0023] Figure 2 is an exploded view of Figure 1.

[0024] Figure 3 is a schematic diagram of the structure of the first solution storage device.

[0025] Figure 4 is a cross-sectional view of the first solution storage unit.

[0026] Figure 5 is a cross-sectional view of the second solution storage unit.

[0027] Figure 6 is a schematic diagram of the puncture component structure.

[0028] Figure 7 is a schematic diagram of the puncture component structure.

[0029] Figure 8 is a cross-sectional view of the microfluidic cartridge in its initial state.

[0030] Figure 9 is a cross-sectional view of the microfluidic cartridge in the second state.

[0031] Figure 10 is a cross-sectional view of the microfluidic cartridge in the third state.

[0032] Figure 11 is a cross-sectional view of the reaction vessel.

[0033] Numbering on the map:

[0034] 1-First solution storage component, 101-First chamber, 102-Second chamber, 103-Third chamber, 104-Fourth chamber, 105-Fifth chamber, 106-First limiting part, 107-Separator; 2-Piercing component, 201-Second limiting part, 202-Third limiting part, 203-First groove, 204-First channel, 205-Second channel, 206-Third channel, 207-Second groove, 208- Fourth limiting part, 209-needle, 210-third groove, 211-fourth channel; 3-second solution storage component, 301-sixth chamber, 302-seventh chamber, 303-eighth chamber, 304-fifth channel, 305-sixth channel; 4-reaction component, 401-ninth chamber, 402-tenth chamber, 403-eleventh chamber, 404-seventh channel, 405-eighth channel, 406-ninth channel. Detailed Implementation

[0035] The technical solution of the present invention will now be described in detail with reference to the accompanying drawings and embodiments.

[0036] The microfluidic cartridge includes a first solution storage unit 1, a puncture unit 2, a second solution storage unit 3, and a reaction unit 4;

[0037] The first solution storage device 1 is provided with a first chamber 101, a second chamber 102, a third chamber 103, a fourth chamber 104, and a fifth chamber 105. The bottom of the first chamber 101 is connected to the bottom of the fourth chamber 104 and the top of the fifth chamber 105. The bottom of the second chamber 102 is connected to the bottom of the third chamber 103. The bottom of the third chamber 103 is connected to the bottom of the fourth chamber 104. The bottom of the fourth chamber 104 is connected to the top of the fifth chamber 105. The top of the fifth chamber 105 is provided with a first limiting part 106. A sealing element is provided on the top surface of the first solution storage device 1, and the first solution storage device 1 is isolated from the outside through the sealing element.

[0038] The second chamber 102 is equipped with a washing fluid sac, and the third chamber 103 is equipped with an elution fluid sac.

[0039] The bottom of the second chamber 102 is higher than the bottom of the third chamber 103 (so that the solution in the second chamber 102 can flow smoothly through it).

[0040] The third chamber 103 and the fourth chamber 104 further enter the first channel 204 to clean the nucleic acid adsorbate. The solution in the third chamber 103 will not enter the second chamber 102, which improves the cleanliness of the solution in the third chamber 103 and prevents the solution in the third chamber 103 from overfilling into the second chamber 102, thus avoiding the solution in the second chamber 102 from entering the second chamber 102 and inhibiting the reaction, thereby improving the accuracy of the experiment. The height of the solution required for the experiment after being injected into the first chamber 101 is lower than the bottom of the third chamber 103 (so that the solution in the first chamber 101 will not enter the third chamber 103, improving the cleanliness of the solution in the third chamber 103).

[0041] The first chamber 101 is provided in two or more, and a spacer 107 (for enhancing mechanical strength) is provided between two adjacent first chambers 101.

[0042] The bottom of the second chamber 102 is not connected to the bottom of the first chamber 101 or the bottom of the fourth chamber 104, and the bottom of the first chamber 101 is not connected to the bottom of the third chamber 103. This design prevents the solution in the first chamber 101 from flowing into the second chamber 102 through the bottom of the chamber, and similarly prevents the solution in the first chamber 101 from flowing into the third chamber 103 through the bottom of the chamber. This avoids cross-contamination between the solution in the first chamber 101 and the solutions in the second and third chambers 102 and 103, improves the cleanliness of the solutions in the second and third chambers 102, and ensures the accuracy of the experimental results.

[0043] The top surface of the piercing element 2 extends outward to form a second limiting part 201. A corresponding third limiting part 202 is provided below the second limiting part 201. A first groove 203 is formed between the second limiting part 201 and the third limiting part 202, and a sealing ring is provided within the first groove 203. The piercing element 2 has a first channel 204, a second channel 205, and a third channel 206. The top surface of the piercing element 2 has an opening that extends downward to form the first channel 204. The first channel 204 contains nucleic acid adsorbent for adsorbing nucleic acids. The first channel 204 is connected to the second channel 205, and the inner diameter of the first channel 204 is larger than the inner diameter of the second channel 205. The second channel 205 limits the nucleic acid adsorbent within the first channel 204. The design prevents nucleic acid adsorbates from entering the third channel 206 through the second channel 205 and blocking the third channel 206 during centrifugation, ensuring that different solutions in the first solution storage container 1 can smoothly pass through the third channel 206 and enter the corresponding chamber. The second channel 205 is connected to the third channel 206, and the third channel 206 is connected to the outside. A second groove 207 is provided above the third channel 206, and a sealing ring is provided in the second groove 207. A solid spike is provided below the third channel 206. The solid spike includes a fourth limiting part 208 and a needle 209. The part between the fourth limiting part 208 and the third channel 206 forms a third groove 210, and a sealing ring is provided in the third groove 210. The end of the needle 209 is provided with a fourth channel 211.

[0044] The second solution storage device 3 is provided with a sixth chamber 301, a seventh chamber 302, an eighth chamber 303, a fifth channel 304, and a sixth channel 305. The sixth channel 305 is connected to both the sixth chamber 301 and the seventh chamber 302. The fifth channel 304 is connected to both the sixth chamber 301 and the eighth chamber 303. The inner diameter of the fifth channel 304 is smaller than the diameter of the top surface of the puncture member 2. Thus, the third limiting part 202 of the puncture member 2 is stuck above the fifth channel 304, and the third limiting part 202 of the puncture member 2 cannot enter the eighth chamber 303. The eighth chamber 303 is pre-stored with reaction liquid, and the bottom surface of the eighth chamber 303 is provided with a seal and is isolated from the outside through the seal.

[0045] The reaction vessel 4 is equipped with a ninth chamber 401, a tenth chamber 402, an eleventh chamber 403, a seventh channel 404, an eighth channel 405, and a ninth channel 406. The eighth channel 405 is connected to both the ninth chamber 401 and the tenth chamber 402. The tenth chamber 402 is connected to both the seventh channel 404 and the ninth channel 406. The ninth channel 406 is connected to the eleventh chamber 403. The eighth channel 404 is used to expel air bubbles from the solution in the eleventh chamber 403, preventing the formation of air bubbles in the solution in the eleventh chamber 403 and avoiding errors caused by the volume of air bubbles, thus ensuring the accuracy of the experimental results.

[0046] The two ends of the second solution storage device 3 are respectively fitted onto the bottom of the first solution storage device 1 and the top of the reaction device 4. The second solution storage device 3 is connected to the first solution storage device 1 and the reaction device 4 by snap-fit. The top of the second solution storage device 3 is fitted into the cavity enclosed by the bottom inner wall of the first solution storage chamber 1. The inner diameter of the eighth chamber 303 matches the inner diameter of the seventh channel 404. When the bottom end of the second solution storage device 3 is fitted into the ninth chamber 401 of the reaction device 4, the positions of the eighth chamber 303 and the seventh channel 404 correspond, ensuring that the solution in the eighth chamber 303 can completely flow into the seventh channel 404 during centrifugation, avoiding the solution from entering the gap between the eighth chamber 303 and the seventh channel 404, preventing leakage, ensuring no loss of solution, and guaranteeing the accuracy and reliability of the experimental results.

[0047] Assembly steps of the microfluidic cartridge:

[0048] In the initial state, the second limiting part 201 and the third limiting part 202 of the puncturing member 2 are movably disposed in the fifth chamber 105. The bottom surface of the first limiting part 106 is in contact with the top surface of the second limiting part 201, and the first limiting part 106 plays a positioning and limiting role for the second limiting part 201 of the puncturing member 2.

[0049] During installation, the first step is to push the piercing component 2 into the fifth chamber 105 until the bottom surface of the first limiting part 106 is in contact with the top surface of the second limiting part 201. The sides of the second limiting part 201 and the third limiting part 202 are in contact with the inner wall of the fifth chamber 105. A sealing ring is provided in the first groove 203. The friction between the sealing ring and the inner wall of the fifth chamber 105 is greater than the weight of the piercing component 2. Thus, when the microfluidic card is placed vertically, the piercing component 2 remains in the fifth chamber 105 and remains in the initial state without external force. At this time, the first chamber 101, the third chamber 103, and the fourth chamber 104 are all connected to the first channel 204 of the piercing component 2.

[0050] In the second step, align the needle 209 of the puncture component 2 assembly installed in the first step with the fifth channel 304 of the second solution storage component 3, and align the slot on the first solution storage component 1 and the buckle on the second solution storage component 3. At this time, the third channel 206 is located in the sixth chamber 301 and is connected to the sixth chamber 301. The needle 209 of the puncture component 2 falls completely into the eighth chamber 303. The third groove 210 is located exactly in the fifth channel 304 of the second solution storage component 3. The sealing ring in the third groove 210 is compressed by the inner wall of the fifth channel 304, which isolates the eighth chamber 303 from the outside, so that the solution in the first chamber 101 and the second chamber 102 does not... It will enter the eighth chamber 303, ensuring that the pre-stored reaction liquid in the eighth chamber 303 is not contaminated by the solution flowing out of the first chamber 101 and the second chamber 102, thus ensuring the accuracy of the experimental results. In addition, the sealing ring set in the third groove 210 further increases the friction between the sealing ring and the inner wall of the fifth channel 304. Thus, in the initial state, without external force, it ensures that the sealing ring in the third groove 210 always stays in the fifth channel 304 and remains in the initial state, so that the eighth chamber 303 is always isolated from the outside world, and the pre-stored reaction liquid in the eighth chamber 303 never comes into contact with the outside world, avoiding the reaction liquid from being affected by the outside world and becoming ineffective before the experiment.

[0051] The third step is to align and connect the clips on the second solution storage device 3 installed in the second step with the slots on the reaction device 4. At this time, the bottom end of the second solution storage device 3 is fitted into the ninth chamber 401 of the reaction device 4, and the positions of the eighth chamber 303 and the seventh channel 404 correspond. The microfluidic cartridge installation is complete.

[0052] The microfluidic cartridge workflow is as follows:

[0053] 1. Adding the lysed sample solution: In the initial state, the microfluidic cartridge is placed in a vertical position. The sealing film at the top of the first solution storage device 1 corresponding to the first chamber 101 is punctured. The lysed sample solution is added into the first chamber 101. The microfluidic cartridge is centrifuged. During centrifugation, the microfluidic cartridge is in a horizontal position. Under the action of centrifugal force, the lysed sample solution first flows through the first channel 204. The nucleic acid in the solution is adsorbed onto the nucleic acid adsorbent. Under the action of centrifugal force, the remaining solution further flows through the second channel 205, the third channel 206, and the sixth channel 305, and finally enters the seventh chamber 302. After all the remaining solution has entered the seventh chamber 302, the centrifugation operation is stopped, and the microfluidic cartridge returns to a vertical position.

[0054] During centrifugation, the sealing ring in the third groove 210 is compressed by the inner wall of the fifth channel 304, isolating the eighth chamber 303 from the outside. Therefore, the lysed sample solution will not flow into the eighth chamber 303, ensuring that the pre-stored reaction solution in the eighth chamber 303 is not contaminated by cross-contamination of the lysed sample solution, thus improving the accuracy of the experimental results. In addition, the compression of the sealing ring in the third groove 210 by the inner wall of the fifth channel 304 further increases the friction between the sealing ring and the inner wall of the fifth channel 304. Therefore, during centrifugation, the friction between the puncture piece 2 and the first solution storage chamber 1 and the second solution storage chamber 3 is greater than the centrifugal force, ensuring that the fixed puncture piece 2 does not shift during centrifugation, and the sealing ring does not shift due to friction during centrifugation, ensuring the directional flow of the lysed sample solution into the seventh chamber 302.

[0055] 2. Washing: Puncture the sealing membrane at the top of the first solution storage device 1 corresponding to the second chamber 102, and puncture the liquid sac inside the second chamber 102. Centrifuge the microfluidic cartridge. During centrifugation, the microfluidic cartridge is in a horizontal state. The washing liquid in the liquid sac first flows through the first channel 204 under the action of centrifugal force. The washing liquid cleans the impurities on the nucleic acid adsorbate, and the nucleic acid is retained on the nucleic acid adsorbate. Under the action of centrifugal force, the washing liquid further flows through the second channel 205, the third channel 206, and the sixth channel 305, and finally enters the seventh chamber 302. After all the washing liquid has entered the seventh chamber 302, the centrifugation operation is stopped, and the microfluidic cartridge returns to a vertical state.

[0056] In this step, the sealing ring serves the same purpose as in step 1.

[0057] 3. Washing: The drive rod is placed into the fourth chamber 104 of the first solution storage device 1. External force pushes the drive rod to move the puncture device 2 downward. During this process, the sealing rings in the first groove 203 and the third groove 210 slide against the friction between the inner wall of the fifth chamber 105 and the inner wall of the fifth channel 304, respectively, until the puncture device 2 slides downward to the second state. At this time, the second limiting part 201 and the third limiting part 202 of the puncture device 2 are located in the fifth chamber 105, and the needle 209, the third groove 210, and the third channel 206 of the puncture device 2 are located in the eighth chamber 303. The second groove 207 just slides into the fifth channel 304. The sealing ring in the second groove 207 is squeezed by the inner wall of the fifth channel 304. At this time, the eighth chamber 303 is connected to the third channel 206, and the drive rod is removed from the fourth chamber 104.

[0058] The sealing membrane corresponding to the third chamber 103 on the top surface of the first solution storage chamber is punctured, and the liquid sac inside the third chamber 103 is also punctured. The microfluidic cartridge is then centrifuged. During centrifugation, the microfluidic cartridge is in a horizontal position. The eluent in the liquid sac first flows through the first channel 204 under the action of centrifugal force, eluting the nucleic acid on the nucleic acid adsorbate. Under the action of centrifugal force, the eluent and nucleic acid further enter the second channel 205 and the third channel 206, and finally enter the eighth chamber 303 to mix with the reaction solution in the eighth chamber 303. After all the eluent has entered the eighth chamber 303, the centrifugation operation is stopped, and the microfluidic cartridge returns to a vertical position. A magnetic rod is pre-installed in the eighth chamber 303. The external magnetic rod drive device is activated, and the magnetic rod is driven to move in the eighth chamber 303 to stir the nucleic acid and reaction solution evenly.

[0059] In this step, the sealing ring functions the same as in step 1, ensuring that the puncture part 2 remains in the second state throughout the centrifugation process.

[0060] 4. Reaction: The driving rod is placed into the fourth chamber 104 of the first solution storage device 1. An external force pushes the driving rod to move the piercing part 2 downward. During this process, the sealing ring in the first groove 203 and the sealing ring in the second groove 207 overcome the friction between the inner wall of the fifth chamber 105 and the inner wall of the fifth channel 304, respectively, and slide until the piercing part 2 slides downward to the third state. At this time, the third limiting part 202 of the piercing part 2 is stuck above the fifth channel 304, and the needle 209 pierces the seal at the bottom of the eighth chamber 303. The fourth channel 211 on the needle 209 is connected to the eighth chamber 303 and the seventh channel 404.

[0061] The microfluidic cartridge is centrifuged. During centrifugation, the microfluidic cartridge is in a horizontal position. Under the action of centrifugal force, the nucleic acid and reaction solution in the eighth chamber 303 enter the seventh channel 404 sequentially from the fourth channel 211 and are evenly distributed to the tenth chamber 402. The centrifugal force is further increased, and the nucleic acid and reaction solution in the tenth chamber 402 finally enter the eleventh chamber 403 through the ninth channel 406. Centrifugation is stopped, and the eleventh chamber 403 is heated. The nucleic acid and reaction solution react under the heating condition. The reaction result can be detected by the detection module of the device.

[0062] In this step, the third limiting part 202 is positioned above the fifth channel 304. This design ensures that the puncture part 2 remains in the third state during centrifugation, allowing the nucleic acid and reaction solution to eventually enter the eleventh chamber 403 of the reaction element smoothly.

[0063] In summary, this invention, through the rational design of the microfluidic cartridge structure, enables the solution in the chamber to flow sequentially and directionally to the target chamber during centrifugation, and the solutions do not cross-contaminate during the transfer process. Under the action of centrifugal force, trace amounts of solution can be transferred to the next chamber without residue, avoiding trace residue and ensuring the accuracy of experimental results.

Claims

1. A microfluidic card cartridge, characterized in that, It includes a first solution storage unit, a puncture component, and a second solution storage unit; wherein: the first solution storage unit includes a fifth chamber, and a first limiting part is provided at the top of the fifth chamber; the top surface of the puncture component extends outward to form a second limiting part, the top surface of the second limiting part abuts against the bottom surface of the first limiting part, a third limiting part is provided below the second limiting part, a first groove is formed between the second limiting part and the third limiting part, and the second limiting part and the third limiting part are movably disposed in the fifth chamber through a sealing ring in the first groove; the top surface of the second limiting part has an opening, the opening extends downward to form a first channel, the first channel is connected to the second channel and the third channel in sequence, the third channel is connected to the outside, and the bottom of the third channel is a solid tip. The piercing part, a solid piercing part, includes a fourth limiting part and a needle tip. The portion between the fourth limiting part and the third channel forms a third groove, and a sealing ring is provided in each of the third grooves. The second solution storage component has a sixth chamber at the top and a seventh and eighth chamber at the bottom. The eighth chamber is located directly below the fifth chamber. The sixth and eighth chambers are connected through the fifth channel. During operation, the third channel of the piercing component is located in and connected to the sixth chamber. The needle tip falls entirely into the eighth chamber. The third groove is located in the fifth channel. The sealing ring in the third groove is compressed by the inner wall of the fifth channel, which isolates the eighth chamber from the outside. The sixth and seventh chambers are connected through the sixth channel. Under centrifugal force, the solution flows sequentially through the first, second, and third channels, then through the sixth channel into the seventh chamber, achieving directional flow. The first solution storage unit also includes a first, second, and third chamber. The bottom of the first chamber is connected to the bottom of the fourth and fifth chambers, the bottom of the second and third chambers are connected, and the bottom of the third and fourth chambers are connected. The bottom of the second chamber is higher than the bottom of the third chamber. The bottom of the second chamber is not connected to the bottoms of the first and fourth chambers, nor is the bottom of the first and third chambers connected. The height of the solution after injection into the first chamber is lower than the bottom of the third chamber. The first chamber is used to add the lysed sample solution. The second chamber contains a washing solution reservoir, and the third chamber contains an elution solution reservoir. The first channel contains nucleic acid adsorbent for adsorbing nucleic acids. The inner diameter of the first channel is larger than that of the second channel.

2. The microfluidic card cartridge according to claim 1, characterized in that, A second groove is provided above the third channel, and a sealing ring is provided inside the second groove.

3. The microfluidic card cartridge according to claim 2, characterized in that, The first solution storage device also includes a fourth chamber, the bottom of which is connected to the top of the fifth chamber. During operation, the drive rod is placed in the fourth chamber, and external force pushes the drive rod to move the puncture device downward until the third limiting part of the puncture device is located in the fifth chamber, the needle is located in the eighth chamber, and the second groove just slides into the fifth channel. The sealing ring in the second groove is squeezed by the inner wall of the fifth channel. Under the action of centrifugal force, the solution flows through the first channel, the second channel, and the third channel in sequence before entering the eighth chamber, realizing directional flow.

4. The microfluidic card cartridge according to claim 3, characterized in that, The inner diameter of the fifth channel is smaller than the diameter of the third limiting part of the puncture device, the bottom of the eighth chamber is equipped with a sealing element, and the needle head is equipped with a fourth channel.

5. The microfluidic cartridge according to claim 4, characterized in that, It also includes a reaction element, which is fixedly installed below the second solution storage element. The top of the reaction element is provided with a seventh channel, which is located directly below the eighth chamber and is connected to the lower chamber of the reaction element. During operation, the drive rod is placed in the fourth chamber, and external force pushes the drive rod to move the piercing element downward until the third limiting part is stuck above the fifth channel. The needle pierces the seal at the bottom of the eighth chamber. The fourth channel is connected to the eighth chamber and the seventh channel. Under the action of centrifugal force, the solution flows sequentially through the first channel, the second channel, the third channel, the eighth chamber, and the seventh channel before entering the lower chamber of the reaction element, achieving directional flow.

6. The microfluidic cartridge according to claim 5, characterized in that, The two ends of the second solution storage device are respectively fitted onto the bottom of the first solution storage device and the top of the reaction device. The second solution storage device is connected to the first solution storage device and the reaction device by snap-fit.

7. The microfluidic card cartridge according to claim 5, characterized in that, The lower part of the reaction chamber is provided with a tenth chamber and an eleventh chamber. The tenth chamber and the eleventh chamber are connected to each other through a ninth channel. The eighth channel is used to discharge air bubbles from the solution in the eleventh chamber. The seventh channel is connected to the tenth chamber.

8. The microfluidic cartridge according to claim 5, characterized in that, The eighth chamber contains a pre-stored reaction solution.

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