Composition with anti-aging effect, personal care product and application of composition and personal care product

Abstract

The invention provides a composition with an anti-aging effect. The composition comprises fibroin and lactobionic acid, the mass ratio of the fibroin to the lactobionic acid is (0.02: 10)-(0.2: 1). The invention further provides application of the composition and a personal care product. In the composition provided by the invention, the fibroin and the lactobionic acid are compounded, so that a synergistic anti-aging effect is achieved. Experimental results show that the composition provided by the invention can promote the expression of at least one of type I collagen alpha1 chain, type I collagen alpha2 chain, type II collagen and elastin, has a synergistic effect and an anti-aging effect, and can be used for anti-aging cosmetics.

Description

Technical Field

[0001] This application relates to the field of daily chemical technology, and in particular to a composition with anti-aging effects, personal care products and their applications. Background Technology

[0002] Anti-aging cosmetics are skincare products designed to delay skin aging. They achieve antioxidant, anti-wrinkle, and anti-aging effects by adding active ingredients such as peptides, ceramides, retinol, grape seed extract, centella asiatica extract, and witch hazel extract.

[0003] The mechanisms of anti-aging include promoting collagen and elastin synthesis, neutralizing free radical oxidative damage, inhibiting the release of inflammatory factors, and repairing the stratum corneum barrier. Lactobionic acid is a commonly used active ingredient that acts on the dermis, increasing skin moisture content and elasticity, and has some anti-wrinkle effects. However, its anti-aging performance is not particularly outstanding. Summary of the Invention

[0004] In view of this, the technical problem to be solved by this application is to provide a composition with anti-aging effects, a personal care product and its application. The composition provided by this application can significantly promote the expression of type I collagen α1 chain Colla Ia, type I collagen α2 chain Colla Ib, type II collagen Colla 2 and elastin, and has a synergistic effect.

[0005] This application provides a composition with anti-aging effects, comprising silk protein and lactobionic acid;

[0006] The mass ratio of the fibroin to lactobionic acid is 0.02:10 to 0.2:1.

[0007] The composition provided in this application includes lactobionic acid, which is a polyhydroxy biomimetic acid with the molecular formula C2. 12 H 22 O 12The structural formula is shown in formula (I), and it has broad application prospects in the food, pharmaceutical, and chemical industries. Lactobionic acid has multiple effects such as moisturizing, repairing, anti-aging, and anti-oxidation. For example, lactobionic acid contains eight hydrophilic hydroxyl groups, thus it has good hygroscopicity and can bind water in the epidermis, acting as an effective moisturizer to soften the skin surface and reduce the appearance of fine wrinkles. Furthermore, lactobionic acid can chelate with metal ions, inhibiting the activity of matrix metalloproteinases (MMPs), protecting collagen, improving skin elasticity, and effectively reducing the effects of photoaging. Moreover, lactobionic acid has a significant effect on improving the homeostasis of the epidermal barrier and the function of the stratum corneum, but it does not produce the irritation and stinging side effects similar to traditional hydroxy acids (such as glycolic acid), making it suitable for sensitive skin. This application does not have any special restrictions on the source of the lactobionic acid; commercially available products are acceptable.

[0008] Formula (I).

[0009] The composition provided in this application includes silk core protein, which is a fibrous protein found in silk. Its amino acid sequence is predominantly composed of alternating glycine, alanine, and serine, containing small amounts of serine and other amino acids, forming a β-sheet core structure. It strengthens the stratum corneum and fortifies the keratinized membrane by promoting the production of three major barrier proteins: tight junction proteins, clasping proteins, and filaggrin. Furthermore, the tyrosine and other amino acids contained in silk core protein, along with its unique β-sheet structure, possess strong antioxidant capabilities and can effectively scavenge free radicals. In some specific implementations, the number-average molecular weight of the silk core protein is 10 kDa to 400 kDa. This application does not impose any special restrictions on the source of the silk core protein; it can be purchased commercially. In some specific implementations, the silk core protein can also be prepared by the following method: degumming of silk by heating, extraction by concentrated salt dissolution, dialysis, and centrifugation.

[0010] In this application, the mass ratio of the silk protein to lactobionic acid is 0.02:10 to 0.2:1, preferably 0.02:6 to 0.1:1, more preferably 0.02:6 to 0.02:1, such as 0.1:1, 0.06:1, 0.02:3, 0.02:2 or 0.02:6, etc.

[0011] The composition provided in this application, in which lactobionic acid and fibroin are used in combination, can promote the expression of at least one of type I collagen α1 chain, type I collagen α2 chain, type II collagen, and elastin, and has a synergistic effect. In some specific implementations, the composition provided in this application can promote the expression of type I collagen α1 chain, type I collagen α2 chain, type II collagen, and elastin, and has anti-aging effects, and can be used in anti-aging cosmetics.

[0012] This application also provides a personal care product, including the anti-aging composition described in the above technical solution.

[0013] In some embodiments of the present invention, the personal care products described above include, in addition to the composition described in the above technical solutions, other excipients, such as excipients for personal care products.

[0014] In some embodiments of the present invention, the personal care products include: basic care products and / or makeup products.

[0015] In some embodiments of the present invention, the cosmetic products include, but are not limited to:

[0016] (1) Base makeup:

[0017] Foundation / cream: Used to even out skin tone and cover blemishes;

[0018] BB cream / CC cream: A lightweight base makeup product that combines skincare and makeup application;

[0019] Concealer / pen: Used to cover up specific areas such as pimples and dark circles;

[0020] Loose powder / setting powder: Sets makeup and reduces facial shine.

[0021] (2) Eye makeup:

[0022] Eyeshadow: Adds color and dimension to the eyes;

[0023] Eyeliner pencil / liquid / gel: Used to draw lines on the eyes, making them look more vibrant;

[0024] Mascara: Lengthens and thickens eyelashes, enhancing the depth of the eyes;

[0025] Eyebrow pencil / powder / gel: Fill in gaps in eyebrows and create the ideal eyebrow shape.

[0026] (3) Cheek makeup:

[0027] Blush: Adds natural color to the cheeks and improves complexion;

[0028] Contouring powder / stick: Use shading techniques to make facial contours more three-dimensional;

[0029] (4) Lip makeup:

[0030] Lipstick / lip gloss / lip tint: to change or emphasize the color of the lips;

[0031] Lip liner: Defines the clear outline of the lips and prevents lipstick from bleeding.

[0032] (5) Multifunctional cosmetics:

[0033] Highlighter sticks / liquids / powders: Highlight high points on the face (such as the bridge of the nose and cheekbones) to create a luminous effect.

[0034] In addition, there are products designed specifically for special occasions, such as waterproof and sweatproof eyeliners and long-lasting, non-fading lipsticks.

[0035] In some embodiments of the present invention, the basic care products include, but are not limited to:

[0036] Facial cleanser: Gently removes dirt, oil, and makeup residue from the face;

[0037] Cleansing oil / cleansing water / cleansing balm: specially designed to thoroughly remove makeup, especially waterproof cosmetics;

[0038] Toner / lotion: Used after cleansing, it can further cleanse the skin's surface of residue, while replenishing the skin's moisture, restoring the skin's pH balance, and laying a good foundation for the absorption of subsequent skin care products;

[0039] Serum: Contains a high concentration of active ingredients, providing deep nourishment and repair for specific skin problems (such as anti-aging, moisturizing, whitening, etc.);

[0040] Eye cream: Specially designed for the sensitive area around the eyes, it has the effects of reducing fine lines and dark circles and firming the skin around the eyes. The texture is usually light and easily absorbed.

[0041] Day / night lotion or cream: It has functions such as protection, repair and nourishment, and promotes cell regeneration. It can provide the skin with the necessary moisture and lock in the replenished moisture.

[0042] Sunscreen: Used for ultraviolet protection and prevention of photoaging, etc.

[0043] Face masks: provide extra nourishment and care for the skin, such as hydration, pore cleansing, or brightening of the complexion.

[0044] This application adds fibroin and lactobionic acid to the above-mentioned products, which can significantly promote the expression of type I collagen α1 chain Colla Ia, type I collagen α2 chain Colla Ib, type II collagen Colla 2 and elastin, and has a synergistic effect.

[0045] This application does not impose any special restrictions on the excipients in the product; they can be added according to the product type. This application also does not impose any special restrictions on the preparation method of the product; the preparation method can be adjusted according to the specific substances selected in the product formulation.

[0046] This application provides a composition with anti-aging effects, comprising fibroin and lactobionic acid; the mass ratio of fibroin to lactobionic acid is 0.02:10 to 0.2:1. Experimental results show that the composition provided in this application can significantly promote the expression of type I collagen α1 chain Colla Ia, type I collagen α2 chain Colla Ib, type II collagen Colla 2, and elastin, and has a synergistic effect, thus possessing anti-aging efficacy and can be used in anti-aging cosmetics. Furthermore, the composition provided in this application is mild and non-irritating. Detailed Implementation

[0047] This invention provides a composition, personal care product, and its application with anti-aging effects. Those skilled in the art can refer to the content of this document and appropriately modify the process parameters to achieve the desired results. The methods and applications of this invention have been described through preferred embodiments. Those skilled in the art can clearly modify or appropriately change and combine the methods and applications described herein without departing from the content, spirit, and scope of this invention to realize and apply the technology of this invention.

[0048] The terms “including,” “having,” or “containing,” including the use of their grammatical synonyms, should generally be understood as open-ended and non-restrictive, for example, not excluding other unstated elements or steps, unless otherwise specifically stated or understood from the context.

[0049] It should be understood that the order of the steps or the order in which certain actions are performed is not important as long as the invention remains operational. Furthermore, two or more steps or actions can be performed simultaneously.

[0050] The use of any and all instances or exemplary language such as “e.g.” or “including” in this document is merely intended to better illustrate the invention and is not intended to limit the scope of the invention unless the claims are made. No language in this specification should be construed as indicating that any unclaimed element is essential to the practice of the invention.

[0051] Furthermore, the numerical ranges and parameters used to define the present invention are approximate values, and the relevant values ​​in the specific embodiments have been presented as precisely as possible. However, any value inevitably contains standard deviations due to individual test methods. Therefore, unless explicitly stated otherwise, it should be understood that all ranges, quantities, values, and percentages used in this disclosure are modified with the word "approximately". Here, "approximately" generally means that the actual value is within plus or minus 10%, 5%, 1%, or 0.5% of a specific value or range.

[0052] This application provides a composition with anti-aging effects, comprising silk protein and lactobionic acid.

[0053] In some specific implementations, the mass ratio of the silk protein to lactobionic acid is 0.02:10 to 0.2:1, preferably 0.02:6 to 0.1:1, and more preferably 0.1:1, 0.06:1, 0.02:3, 0.02:2 or 0.02:6.

[0054] The composition provided in this application can promote the expression of at least one of type I collagen α1 chain, type I collagen α2 chain, type II collagen, and elastin, and has a synergistic effect. In some specific implementations, the composition provided in this application can promote the expression of type I collagen α1 chain, type I collagen α2 chain, type II collagen, and elastin, and has anti-aging effects, and can be used in anti-aging cosmetics.

[0055] The present application will be further described below with reference to the embodiments.

[0056] In the following examples, the silk protein raw material is a silk protein aqueous solution purchased from the market, with a silk protein content of 2wt% and a number average molecular weight of 10kDa~400kDa.

[0057] Examples 1-5, Comparative Examples 1-1-5-2

[0058] Prepare the composition according to the formulation shown in Table 1:

[0059]

[0060] The preparation method is as follows:

[0061] Mix the fibroin aqueous solution and lactobionic acid in a certain proportion.

[0062] Test case

[0063] The compositions provided in Examples 1-5 and Comparative Examples 1-1-5-2 were tested according to the following methods, and the test concentrations for each group are shown in Table 2. Taking Example 1 as an example, the tests were conducted according to the following methods:

[0064] 1.1 Test Grouping

[0065] Healthy zebrafish, 6 days old after fertilization, were selected and set up as a blank control group (fish embryo culture medium), a model control group (fish embryo culture medium + UVB irradiation) and Example 1 group (Example 1 composition + UVB irradiation).

[0066] Blank control group: 36 zebrafish were randomly selected and evenly distributed into 3 6cm culture dishes. Each culture dish contained 12 zebrafish and 6mL of fish embryo culture medium.

[0067] Model control group: 36 zebrafish were randomly selected and evenly distributed into 3 6cm culture dishes, each containing 12 zebrafish and 6mL of fish embryo culture medium;

[0068] Example 1: 36 zebrafish were randomly selected and evenly distributed into 3 6cm culture dishes. Each culture dish contained 12 zebrafish and 6mL of fish embryo culture medium.

[0069] Each group was incubated in a constant temperature incubator at 28℃±1℃ for 1 hour. Zebrafish were anesthetized with tricaine, and the corresponding test solution was changed according to the group. Then, 0.3J / cm² was applied. 2 After UVB irradiation, the anesthetic was removed and the cells were incubated in a constant temperature incubator at 28℃±1℃ for 24h±1h.

[0070] Collect 12 zebrafish from each culture dish into a 1.5 mL tube, remove the solution, add 0.5 mL of RNAlater solution, and store frozen.

[0071] 1.2 RNA extraction

[0072] Remove the RNAlater solution, wash three times with PBS, place on ice, remove the PBS, and add 500 μL of TRIzol reagent. Homogenize the embryos using a pellet mill, place on ice for 10 min, add 100 μL of chloroform, vortex for 1 min, and place on ice for 5 min. Centrifuge the sample for 20 min, transfer the supernatant to a 1.5 mL centrifuge tube, add 250 μL of isopropanol, vortex for 1 min, place on ice for 10 min, and centrifuge for 20 min. Remove all supernatant, add 500 μL of ethanol, vortex for 1 min, centrifuge for 5 min, and place on ice for 10 min. Repeat this step three times. Remove the ethanol, centrifuge the sample for 5 min, air dry the sample for 10 min, add 10 μL of DNase / RNase-free ultrapure distilled water, and heat at 55°C for 15 min.

[0073] 1.3 cDNA Synthesis

[0074] The RNA sample was diluted to 1000 ng / 7 μL with DNase / RNase-free ultrapure distilled water, and the RNA was synthesized into cDNA using the PrimeScriptRT kit (Takara; Cat no. RR047A) containing gDNA Eraser. The cDNA was then stored frozen.

[0075] 1.4 RT-PCR

[0076] Prepare primer mixtures and real-time PCR master mixtures for each primer. Dilute cDNA samples 10-fold with DNase / RNase-free ultrapure distilled water. Add 9 μL of PCR master mixture and 1 μL of diluted cDNA sample to each well of a 96-well PCR plate, seal the 96-well PCR plate with optical sealant, centrifuge the plate for 5 min, and perform real-time PCR amplification.

[0077] 1.5 Data and Result Calculation

[0078] Real-time PCR data were collected, with Ct as the amplification result. β-actin was used as the housekeeping gene, and the relative expression levels of each gene (including elastin and collagen (Colla Ia, Colla Ib, and Colla II)) were calculated as the test results.

[0079]

[0080] In the formula:

[0081] ΔCt: The difference between the cycle number of the target gene and the cycle number of the housekeeping gene;

[0082] The number of cycles required for the target gene fluorescence signal to reach a set threshold;

[0083] The number of cycles required for the housekeeper gene fluorescence signal to reach a set threshold;

[0084] ΔCt: The difference between the average ΔCt of the model control group and the ΔCt of Example 1 group;

[0085] ΔCt 模型对照组 The difference between the cycle number of the target gene and the cycle number of the housekeeping gene in the model control group;

[0086] ΔCt 受试物 Example 1: The difference between the cycle number of the target gene and the cycle number of the housekeeping gene;

[0087] 2 -ΔΔCt 模型对照组 : Relative expression level of the target gene in the model control group;

[0088] 2 -ΔΔCt 受试物 Example 1: Relative expression levels of the target gene.

[0089] The mean and standard error of each experimental group were calculated, and the statistical results were expressed as mean ± standard error. Analysis of variance was performed on the data using statistical software. A two-tailed t-test was conducted on the relative expression levels of the target gene between the Example 1 group and the model control group, and the p-value was calculated. The results are shown in Table 2, which presents the test results of the compositions provided in the examples and comparative examples of this application.

[0090] Table 2 Test results of the compositions provided in the embodiments and comparative examples of this application

[0091]

[0092] In Table 2, the synergy coefficients are calculated according to the King's method:

[0093] q=E A+B / (E A +E B -E A *E B ).

[0094] As shown in Table 2, the composition provided in this application can significantly promote the expression of type I collagen α1 chain Colla Ia, type I collagen α2 chain Colla Ib, type II collagen Colla 2 and elastin, and has a synergistic effect. Therefore, the composition provided in this application has good anti-aging effects.

[0095] The primer sequences are shown in Table 3, which contains the primer sequences for PCR amplification in the embodiments of this application.

[0096] Table 3 Primer sequences for PCR amplification in the embodiments of this application

[0097]

[0098] The above are merely preferred embodiments of the present invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A composition having an anti-aging effect, comprising silk fibroin and lactobionic acid; a mass ratio of the silk fibroin and the lactobionic acid is 0.02:10 to 0.2:

1.

2. The composition of claim 1, wherein, a mass ratio of the silk fibroin and the lactobionic acid is 0.02:6 to 0.1:

1.

3. The composition of claim 2, wherein, a mass ratio of the silk fibroin and the lactobionic acid is 0.1:1, 0.06:1, 0.02:3, 0.02:2 or 0.02:

6.

4. The composition according to any one of claims 1 to 3, characterized in that, a number average molecular weight of the silk fibroin is 10 kDa to 400 kDa. 5.Use of the composition in the preparation of a cosmetic product having an anti-aging effect, the composition comprising silk fibroin and lactobionic acid; a mass ratio of the silk fibroin and the lactobionic acid is 0.02:10 to 0.2:

1.

6. Use according to claim 5, characterized in that, the anti-aging is promoting expression of at least one of collagen type I alpha 1 chain, collagen type I alpha 2 chain, collagen type II and elastin.

7. Use according to claim 6, characterized in that, the anti-aging is promoting expression of collagen type I alpha 1 chain, collagen type I alpha 2 chain, collagen type II and elastin.

8. Use according to claim 5, characterized in that, a mass ratio of the silk fibroin and the lactobionic acid is 0.02:6 to 0.1:

1.

9. Use according to claim 8, characterized in that, a mass ratio of the silk fibroin and the lactobionic acid is 0.1:1, 0.06:1, 0.02:3, 0.02:2 or 0.02:

6. 10.A personal care product comprising the composition having an anti-aging effect according to any one of claims 1 to 4.

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