A yanan yellow cattle bone-derived type i collagen, and a preparation method and application thereof
High-purity type I collagen from Yanbian cattle bone was prepared through steps such as gradient acid immersion, layered screening, and pepsin enzymatic hydrolysis, which solved the problem of resource waste and expanded the scope of application.
CN121537505BActive Publication Date: 2026-07-24YANBIAN UNIV
Patent Information
- Application Number
- CN202610083873.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2026-01-22
- Publication Date
- 2026-07-24
- Estimated Expiration
- 2046-01-22
AI Technical Summary
Technical Problem
Existing research has not successfully prepared type I collagen from Yanbian cattle bones, and the resources of Yanbian cattle bones have not been fully utilized, resulting in resource waste and a narrow range of applications.
Method used
An extraction scheme tailored to the characteristics of Yanbian cattle bones was designed using steps such as gradient acid immersion, layered screening, pepsin hydrolysis, and salting out, preserving the complete triple helix structure of collagen.
Benefits of technology
High-purity type I collagen was successfully extracted, solving the problem of resource waste, expanding the application scope of Yanbian cattle, and realizing high-value utilization.
✦ Generated by Eureka AI based on patent content.
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Figure CN121537505B_ABST
Abstract
The application belongs to the technical field of biomedical raw materials, and particularly relates to a Yanbian yellow cattle bone-derived type I collagen as well as a preparation method and application thereof. The preparation method comprises the following steps: crushing and layering and screening fat-removed Yanbian yellow cattle bones to obtain Yanbian yellow cattle bone particles, gradiently immersing the Yanbian yellow cattle bone particles in acid to obtain mineral-removed Yanbian yellow cattle bone particles, and then removing impure proteins; using pepsin for enzymolysis to obtain an enzymolysis liquid, and then performing salting-out to obtain a crude extract; and further purifying the obtained crude extract to obtain the Yanbian yellow cattle bone-derived type I collagen. The preparation method effectively removes inorganic salts (hydroxyapatite), fat and non-collagen protein impurities in bone tissues, and the content of the finally obtained type I collagen is greater than or equal to 800 mg / g, and the product has high purity.
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Citation Information
Patent Citations
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