Optical assembly capable of forming double annular focused light beams, spectrum detection device and method

Abstract

The invention relates to an optical assembly capable of forming a double-ring-shaped focusing light beam, a spectrum detection device and a spectrum detection method. The method comprises the following steps: converting laser into a flat-topped light beam, converting the flat-topped light beam into a double-ring-shaped focusing light beam, and forming a double-ring-shaped focusing light spot on a focusing plane by the double-ring-shaped focusing light beam; a multi-parameter immunochromatography test strip is arranged, the plane where the multi-parameter immunochromatography test strip is located coincides with the focusing plane, and a detection line and a quality control line arranged on the multi-parameter immunochromatography test strip form an inner annular structure and an outer annular structure respectively; after a sample is loaded on the multi-parameter immunochromatography test strip, an inner annular focusing light spot acts on a detection target object fixed on a detection line to excite corresponding surface Raman scattering; the outer annular focusing light spot acts on a molecular probe fixed on a quality control line to excite corresponding surface Raman scattering; and carrying out spectral analysis on the Raman scattering light. According to the invention, the problem of simultaneous high-sensitivity detection of various to-be-detected targets is solved, and the detection efficiency is improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of laser spectrum precision measurement, and in particular to a spectrum detection device and method based on a double-ring focusing light beam. BACKGROUND

[0002] Raman spectrum technology, as a non-invasive optical analysis method, obtains molecular vibration and rotation information by detecting the frequency difference (Raman shift) between scattered light and incident light, thereby realizing accurate analysis of the composition and structure of matter. Raman spectrum technology has the advantages of being fast, accurate, non-destructive, etc. With the iteration of technology, its application has expanded to multiple fields such as chemical analysis, biomedical diagnosis, material science, environmental monitoring, etc., and it has shown unique value in scenarios such as drug component positioning, tumor tissue identification, and cell component analysis.

[0003] Traditional Raman spectrum systems use single-path excitation design, which has inherent defects such as low utilization rate of excitation light and limited detection sensitivity, especially in the detection of trace substances, which is easily affected by fluorescence interference and thermal effects. Moreover, traditional Raman spectrum systems measure single targets at a single point, which cannot meet the demand of simultaneous analysis of multiple components in actual detection, and has obvious shortcomings in high-throughput joint detection and complex system characterization. SUMMARY

[0004] Therefore, the present application provides an optical assembly capable of forming a double-ring focusing light beam, a spectrum detection device and method, which solve the above technical problems.

[0005] The present application realizes the following technical scheme, an optical assembly capable of forming a double-ring focusing light beam, comprising a first conical mirror, a second conical mirror and a third conical mirror, all of which are conical bodies, and a first parabolic cylindrical mirror and a second parabolic cylindrical mirror, both of which are hollow cylindrical bodies; the inner side of the first parabolic cylindrical mirror and the second parabolic cylindrical mirror can respectively focus light perpendicular to the axis of the cylindrical body to the same point; the first conical mirror, the second conical mirror and the third conical mirror are coaxially arranged in sequence, the conical angle end of the first conical mirror faces the direction of light incidence, and the conical angle ends of the second conical mirror and the third conical mirror face in the opposite direction to the conical angle end of the first conical mirror; the first parabolic cylindrical mirror is coaxially sleeved outside the first conical mirror, and the second parabolic cylindrical mirror is coaxially sleeved outside the first parabolic cylindrical mirror; a flat-top light beam is incident to the side surface of the first conical mirror parallel to the axis of the conical body, and after being reflected, it is incident to the curved surface of the first parabolic cylindrical mirror in a direction perpendicular to the axis of the conical body; the reflected light is incident to the side surface of the second conical mirror, and forms an outer ring-shaped focusing light beam after being reflected by the side surface of the second conical mirror; the light transmitted by the first parabolic cylindrical mirror is incident to the curved surface of the second parabolic cylindrical mirror, and after being reflected, it is incident to the side surface of the third conical mirror, and forms an inner ring-shaped focusing light beam after being reflected by the side surface of the third conical mirror, and the inner and outer ring-shaped focusing light beams constitute a double-ring focusing light beam. Further, the inner and outer annular focusing light beams are focused to the same focusing plane, and the double annular focusing light beams are focused to double annular focusing light spots in the focusing plane.

[0006] Further, the inner side of the cylinder is a curved surface formed by rotating a partial parabola around its axis, the directrix of the partial parabola is parallel to the axis of the cylinder, and the focus of the partial parabola is located on the axis of the cylinder.

[0007] A spectrum detection device based on double annular focusing light beams, comprising the optical assembly, the laser, the shaping module, the first filter, the object table, the spectrum analysis module and the multi-parameter immunochromatography test strip as described above; the laser emits laser light, and the shaping module is used for converting the laser light into a flat-top light beam; after the flat-top light beam filters out stray light and interference light through the first filter, the double annular focusing light beam is converted through the optical assembly; the double annular focusing light beam forms a double annular focusing light spot including inner and outer annular focusing light spots in the focusing plane; the multi-parameter immunochromatography test strip is composed of a plurality of immunochromatography test strips arranged in a radial manner on the object table, and the detection lines and the quality control lines provided on the plurality of immunochromatography test strips respectively form inner and outer annular structures; the plane where the multi-parameter immunochromatography test strip is located coincides with the focusing plane, the inner and outer annular focusing light spots of the double annular focusing light spot correspondingly irradiate on the inner and outer annular structures, respectively act on a plurality of detection target objects fixed on the detection lines and molecular probes fixed on a plurality of quality control lines, and respectively excite corresponding Raman scattering light; after the Raman scattering light exits to the first filter to remove interference light through the optical assembly, the Raman scattering light exits to the spectrum analysis module for spectrum analysis.

[0008] Further, the inner side of the first parabolic cylinder mirror is coated with a light splitting film, so that the light intensity of the light reflected by the inner side of the first parabolic cylinder mirror is less than the light intensity of the light transmitted through the inner side of the first parabolic cylinder mirror.

[0009] Further, the outer side of the first parabolic cylinder mirror is coated with an anti-reflection film.

[0010] Further, the curved surface of the second parabolic cylinder mirror is coated with an anti-reflection film.

[0011] Further, the spectrum detection device further comprises a motor driving mechanism connected with the second conical mirror and the third conical mirror, and the motor driving mechanism is used for driving the second conical mirror and / or the third conical mirror to move along the axis direction thereof.

[0012] A double-ring focusing light beam spectrum detection method, which is specifically as follows: converting laser into a flat-top light beam and removing stray light; converting the flat-top light beam into a double-ring focusing light beam, the double-ring focusing light beam forming a double-ring focusing light spot including inner and outer ring-shaped focusing light spots on a focusing plane; setting a multi-parameter immunochromatography test strip, so that the plane where the multi-parameter immunochromatography test strip is located coincides with the focusing plane, and the detection line and the quality control line provided on the multi-parameter immunochromatography test strip form inner and outer ring-shaped structures, respectively; after the multi-parameter immunochromatography test strip is loaded, the inner ring-shaped focusing light spot acts on the detection target fixed on the detection line to excite corresponding surface Raman scattering, and the outer ring-shaped focusing light spot acts on the molecular probe fixed on the quality control line to excite corresponding surface Raman scattering; and whether there is a detection target and whether the immunochromatography test strip is effective are determined by performing spectrum analysis on the Raman scattering light.

[0013] Further, the optical assembly described above is used to convert the flat-top light beam into a double-ring focusing light beam.

[0014] Compared with the prior art, the present application has the following beneficial effects: 1. The present application uses three conical mirrors and two parabolic mirrors, the first, second and third conical mirrors are coaxially arranged in sequence, the first parabolic cylinder mirror is coaxially sleeved outside the first conical mirror, and the second parabolic cylinder mirror is coaxially sleeved outside the first parabolic cylinder mirror, so as to convert the flat-top light beam into a double-ring focusing light beam.

[0015] 2. The inner side of the two parabolic mirrors is a curved surface formed by a partial parabola rotating one revolution around the axis, the directrix of the partial parabola is parallel to the axis of the cylinder, and the focus of the partial parabola is located on the axis of the cylinder, so that the flat-top light beam incident to the inner side of the parabolic mirror is strictly focused to the focus after reflection, at this time, the conical mirror is designed in the optical path, so as to make the reflected flat-top light beam incident to the conical mirror, and the purpose of forming a ring-shaped focusing light beam is achieved.

[0016] 3. The present application realizes high-intensity ring-shaped light spots on the focusing plane through precise optical design, and the spatial distribution can be geometrically matched with the detection line and the quality control line of the immunochromatography test strip, so as to solve the problem of simultaneously detecting multiple target objects with high sensitivity and improve the detection efficiency.

[0017] 4. Compared with the traditional single-point measurement, the present application covers the detection area with a ring-shaped light spot, the utilization rate of excitation light is high, the effective action area of excitation light and sample is improved, and the hot spot excitation precision is improved by 2-3 orders of magnitude.

[0018] 5. The present invention achieves a first reflected light intensity less than the first transmitted light intensity by coating a beam-splitting film on the incident surface of the first parabolic cylindrical mirror; and an anti-reflection film is coated on the outer surface of the first parabolic cylindrical mirror, and an anti-reflection film is coated on the curved surface of the second parabolic cylindrical mirror, ensuring that the light intensity of the inner annular focused spot acting on the detection line is stronger than the light intensity of the outer annular focused spot acting on the quality control line, which facilitates subsequent excitation Raman scattering and spectral analysis.

[0019] 6. The motor drive mechanism of the present invention can drive the second conical mirror and / or the third conical mirror to move along their axial direction, so as to achieve precise adjustment of the size, position and focused light power density of the inner ring focused beam and the outer ring focused beam, so as to better align them with the detection line and the quality control line. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the structure of the present invention.

[0021] Figure 2 This is a schematic diagram of the structure of the multi-parameter immunochromatographic test strip of the present invention (the left side is a schematic diagram of 20 immunochromatographic test strips, and the right side is the corresponding double-ring focused beam and the corresponding light ring intensity on the multi-parameter immunochromatographic test strip).

[0022] Figure 3 Figure (a) is a perspective view of the conical mirror of the present invention, and Figure (b) is a filled view of the conical mirror.

[0023] Figure 4 Figure (a) is a perspective view of the parabolic cylindrical mirror of the present invention, and Figure (b) is a filled view of the parabolic cylindrical mirror.

[0024] Among them, 1-laser; 2-first collimating lens; 3-first shaping lens; 4-second shaping lens; 5-first filter; 6-first conical mirror; 7-first parabolic cylindrical mirror; 8-second parabolic cylindrical mirror; 9-second conical mirror; 10-third conical mirror; 11-multi-parameter immunochromatographic test strip; 12-stage; 13-first focusing lens; 14-slit; 15-second collimating lens; 16-grating; 17-second focusing lens; 18-second filter; 19-CCD detector; 20-analysis control system. Detailed Implementation

[0025] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0026] Example 1 This embodiment provides an optical component capable of forming a dual-ring focused beam, such as... Figure 1As shown, the optical assembly includes a first conical mirror 6, a first parabolic cylindrical mirror 7, a second parabolic cylindrical mirror 8, a second conical mirror 9, and a third conical mirror 10.

[0027] like Figure 3 As shown, the first conical mirror 6, the second conical mirror 9, and the third conical mirror 10 are all conical mirrors with similar structures, namely, all three are conical structures. The cone angle and height of the three are determined according to the specific application. The side of the conical structure is used to reflect the incident light. The first conical mirror 6, the second conical mirror 9, and the third conical mirror 10 are arranged in sequence and are coaxial.

[0028] The cone-shaped end of the first conical mirror 6 faces the direction of light incidence, while the cone-shaped ends of the second conical mirror 9 and the third conical mirror 10 face opposite directions to the cone-shaped end of the first conical mirror 6. The sides of the first conical mirror 6, the second conical mirror 9, and the third conical mirror 10 are used to reflect the incident light. A flat-top beam with its optical axis parallel to the axis of the first conical mirror 6 is incident on the side of the first conical mirror 6 and reflected by the side of the first conical mirror 6 to form a hollow beam that exits perpendicular to the axis of the conical mirror.

[0029] like Figure 4 As shown, both the first parabolic cylindrical mirror 7 and the second parabolic cylindrical mirror 8 are parabolic cylindrical mirrors with similar structures. Both are hollow cylindrical structures, coaxial with the conical mirror. The outer surface of the cylindrical structure is a circular surface, and the inner surface is a curved surface formed by rotating a partial parabola around the axis of the cylindrical structure. The directrix of this partial parabola is parallel to the axis of the cylindrical structure, and the focal point of this partial parabola is located on the axis of the cylindrical structure. The parabolic cylindrical mirror can reflect light incident on its curved surface perpendicular to the directrix, and focus the emitted light at the focal point.

[0030] The first parabolic cylindrical mirror 7 is coaxially sleeved outside the first conical mirror 6, and the second parabolic cylindrical mirror 8 is coaxially sleeved outside the first parabolic cylindrical mirror 7. The first parabolic cylindrical mirror 7 receives the hollow light beam reflected from the side of the first conical mirror 6. The curved surface of the first parabolic cylindrical mirror 7 is the reflecting surface of the incident light. That is, the hollow light beam reflected from the first conical mirror 6 is perpendicular to the axis of the conical mirror (perpendicular to the directrix of the parabolic cylindrical mirror) and is incident on the curved surface of the first parabolic cylindrical mirror 7. The focal point of the light reflected from the curved surface of the first parabolic cylindrical mirror 7 is located on the axis of the conical mirror on the side where the second conical mirror 9 is located. The light reflected from the curved surface of the first parabolic cylindrical mirror 7 is incident on the side of the second conical mirror 9 and is reflected by the side of the second conical mirror 9 to form an outer ring focused beam, forming an outer ring focused spot on the first focusing plane. The outer ring focused beam (outer ring focused spot) is coaxial with the axis of the conical mirror.

[0031] The second parabolic cylindrical mirror 8 receives the hollow light beam transmitted through the first parabolic cylindrical mirror 7. The curved surface of the second parabolic cylindrical mirror 8 is the reflecting surface of the incident light. That is, the hollow light beam emitted from the first parabolic cylindrical mirror 7 is perpendicular to the axis of the conical mirror (perpendicular to the directrix of the parabolic cylindrical mirror) and enters the curved surface of the second parabolic cylindrical mirror 8. The focal point of the light reflected from the curved surface of the second parabolic cylindrical mirror 8 is located on the axis of the conical mirror on the side where the third conical mirror 10 is located. The light reflected from the curved surface of the second parabolic cylindrical mirror 8 enters the side surface of the third conical mirror 10 and is reflected by the side surface of the third conical mirror 10 to form an inner annular focused beam, forming an inner annular focused spot on the second focusing plane. The inner annular focused beam (inner annular focused spot) is coaxial with the axis of the conical mirror; the diameter of the inner annular focused spot is smaller than that of the outer annular focused spot. The second focusing plane and the first focusing plane are the same focusing plane.

[0032] The outer and inner ring-shaped focused beams constitute a double-ring focused beam. In specific implementations, those skilled in the art can design the curvature of the first parabolic cylindrical mirror 7 and the reflecting surface 8 of the second parabolic cylindrical mirror, the distance between them along the direction perpendicular to the axis of the conical mirror, and the cone angles of the second conical mirror 9 and the third conical mirror 10 to achieve focusing of the outer and inner ring-shaped focused beams on the same focusing plane (the first and second focusing planes are the same plane), thus forming a double-ring focused spot. At this time, the outer ring-shaped focused spot is the outer ring-shaped focused spot, and the inner ring-shaped focused spot is the inner ring-shaped focused spot. Among them, the outer ring-shaped focused spot, the inner ring-shaped focused spot, the conical mirror, and the parabolic cylindrical mirror are coaxial, and this axis is also the normal to the focusing plane.

[0033] The optical path of the optical component is as follows: a flat-top beam with its optical axis parallel to the axis of the first conical mirror 6 is incident on the side of the first conical mirror 6 and reflected in a direction perpendicular to the axis of the first conical mirror 6 onto the curved surface of the first parabolic cylindrical mirror 7. A portion of the beam incident on the curved surface of the first parabolic cylindrical mirror 7 is reflected by the curved surface of the first parabolic cylindrical mirror 7 and is called the first reflected light; another portion of the beam incident on the curved surface of the first parabolic cylindrical mirror 7 passes through the outer side of the first parabolic cylindrical mirror 7 and is called the first transmitted light.

[0034] The first reflected light is incident on the side of the second conical mirror 9, and after being reflected by the side of the second conical mirror 9, it is focused onto the same focal plane to form an outer annular focused light spot. The first transmitted light is incident on the curved surface of the second parabolic cylindrical mirror 8, and after being reflected by the curved surface of the second parabolic cylindrical mirror 8, it is reflected to the side of the third conical mirror 10, and after being reflected by the side of the third conical mirror 10, it is focused onto the same focal plane to form an inner annular focused beam spot.

[0035] In some embodiments, the intensity of the first reflected light and the first transmitted light is controlled by depositing a beam-splitting film with a different beam-splitting ratio on the incident surface (curved surface) of the first parabolic cylindrical mirror 7, such that the deposited beam-splitting film can make the intensity of the first reflected light less than the intensity of the first transmitted light.

[0036] In some embodiments, in order to maximize the penetration of the first transmitted light through the first parabolic cylindrical mirror 7, an antireflective coating is deposited on the outer surface of the first parabolic cylindrical mirror 7.

[0037] In some embodiments, in order to maximize the reflection of the first transmitted light by the incident surface (curved surface) of the second parabolic cylindrical mirror 8, a wide-band anti-reflection coating is deposited on the curved surface of the second parabolic cylindrical mirror 8.

[0038] Example 2 This embodiment provides a spectral detection device based on a dual-ring focusing beam, such as... Figure 1 As shown, the spectral detection device includes optical components, laser 1, shaping module, first filter 5, stage 12, spectral analysis module, and multi-parameter immunochromatographic test strip 11 as described above.

[0039] Laser 1 is used to emit a Gaussian distributed laser beam. A shaping module is used to convert the Gaussian distributed beam into a flat-top beam. In this embodiment, the shaping module includes a first collimating lens 2, a first shaping lens 3, and a second shaping lens 4. The Gaussian distributed beam emitted from laser 1 is collimated by the first collimating lens 2 and then sequentially incident on the first shaping lens 3 and the second shaping lens 4. The collimated Gaussian beam is shaped into a flat-top beam after passing through the first shaping lens 3 and the second shaping lens 4. This part is common knowledge to those skilled in the art and will not be elaborated further. In other embodiments, the shaping module can use an optical shaper or other optical devices, as long as the Gaussian distributed beam is flattened and converted into a flat-top beam.

[0040] The flat-top beam emitted from the second shaping lens 4 passes through the first filter 5. At this time, the first filter 5 is used to filter out stray light and interference light from the flat-top beam. After that, the flat-top beam is incident on the optical component parallel to the axis of the conical mirror. The optical component is used to convert the flat-top beam into a double-ring focused beam and can generate a double-ring focused spot on the focusing plane.

[0041] The multi-parameter immunochromatographic test strip 11 consists of several immunochromatographic test strips. Each immunochromatographic test strip includes, from top to bottom, a sample pad 111, a conjugation pad 112, a nitrocellulose membrane, and an absorbent pad. The nitrocellulose membrane is marked with a test line 113 (T line) and a control line 114 (C line).

[0042] Several immunochromatographic test strips are arranged radially on the stage 12, with the sample pad 111 and conjugate pad 112 located at the center. The detection lines 113 of all the immunochromatographic test strips are connected to form an inner ring structure, and the control lines 114 of all the immunochromatographic test strips are connected to form an outer ring structure. The multi-parameter immunochromatographic test strip 11 on the stage 12 faces the cone end of the third conical mirror 10, and the plane of the multi-parameter immunochromatographic test strip 11 is perpendicular to the axis of the third conical mirror 10. The plane of the multi-parameter immunochromatographic test strip 11 coincides with the focusing plane of the dual-ring focused beam. The inner and outer ring focused beams of the dual-ring focused beam illuminate the inner and outer ring structures of the multi-parameter immunochromatographic test strip 11 on the stage 12.

[0043] The sample solution to be tested is loaded onto the sample pad 111 of each immunochromatographic test strip, penetrating to the conjugation pad 112 below the sample pad 111. If the sample solution contains the target analyte, the target analyte molecules bind to the molecular probe in the conjugation pad 112, forming a "target analyte-labeled antibody" complex. The complex flows to the nitrocellulose membrane of the immunochromatographic test strip through capillary action and diffusion. After passing through the detection lines 113 of each immunochromatographic test strip, different target analyte molecules in the complex are specifically bound to the first antibody in the different detection lines 113 and fixed on the detection lines 113. The remaining molecular probes in the sample solution continue to flow forward and bind to the second antibody on the control line 114, and are fixed on the control line 114.

[0044] like Figure 2 As shown, the inner ring-shaped focused light spot illuminates several ring-shaped detection lines 113 in the multi-parameter immunochromatographic test strip 11, acting on several detection targets fixed on the detection lines 113 and exciting corresponding Raman scattering light; the outer ring-shaped focused light spot illuminates several ring-shaped control lines 114 in the multi-parameter immunochromatographic test strip 11, acting on several molecular probes fixed on the control lines 114 and exciting corresponding Raman scattering light.

[0045] The Raman scattered light is then analyzed by the spectral analysis module to determine the target analyte fixed on the detection line 113 and the validity of the immunochromatographic test strip. Specifically, the Raman scattered light returns to the first filter 5 via the optical components. "Returning via the original path" means that the Raman scattered light passes through the third conical mirror 10, the second parabolic cylindrical mirror 8, the first parabolic cylindrical mirror 7, and the first conical mirror 6, or through the second conical mirror 9, the first parabolic cylindrical mirror 7, and the first conical mirror 6, before being filtered by the first filter 5 to remove interference light and then emitted to the spectral analysis module. The spectral analysis module performs spectral analysis on the Raman scattered light and sends the spectral information to the computer's analysis and control system 20 for processing.

[0046] The spectral analysis module is a technology well known to those skilled in the art. It typically includes focusing, collimating, dispersing, focusing, filtering stray light, detecting, and sending electrical signals to a computer. In this embodiment, the spectral analysis module includes a first focusing lens 13, a slit 14, a second collimating lens 15, a grating 16, a second focusing lens 17, a second filter 18, and a CDD detector 19.

[0047] After the Raman scattered light exits from the first filter 5, it is focused by the first focusing lens 13, enters the second collimating lens 15 through the slit 14, is collimated, dispersed by the grating 16, and then focused by the second focusing lens 17. The optical signal is converted into an electrical signal by the CDD detector 19 and transmitted to the computer analysis and control system 20 for processing. The system determines whether there is a detection target fixed on the detection line 113 and whether the immunochromatographic test strip 11 is effective by determining whether there is a molecular probe fixed on the quality control line 114.

[0048] To facilitate spectral detection by the spectral analysis module, the intensity of the inner annular focused spot acting on the detection line 113 must be stronger than the intensity of the outer annular focused spot acting on the control line 114; that is, the intensity of the first reflected light must be weaker than the intensity of the first transmitted light. In this embodiment, a beam-splitting film is deposited on the incident surface (curved surface) of the first parabolic cylindrical mirror 7 to make the intensity of the first reflected light less than the intensity of the first transmitted light; and an anti-reflection film is deposited on the outer surface of the first parabolic cylindrical mirror 7, and a broadband anti-reflection film is deposited on the curved surface of the second parabolic cylindrical mirror 8.

[0049] As an improvement, the spectral detection device also includes a motor drive mechanism connected to the second conical mirror 9 and the third conical mirror 10. Under the control of the analysis and control system 20, the motor drive mechanism drives the second conical mirror 9 and / or the third conical mirror 10 to move along their axes (i.e., the normal to the focusing plane of the double-ring focused beam). By moving the second conical mirror 9 and the third conical mirror 10 parallel to the normal to the focusing plane, precise adjustment of the size, position, and focused light power density of the inner and outer ring focused beams is achieved, allowing for better alignment with the detection line 113 and the control line 114.

[0050] Example 3 This invention also provides a spectral detection method based on a dual-ring focused beam, comprising the following steps: S1: After converting the laser into a flat-top beam, stray light is removed by a filter.

[0051] S2: The flat-top beam is converted into a double-ring focused beam using the optical components in Example 1. The double-ring focused beam includes an inner ring focused beam and an outer ring focused beam. The inner ring focused beam and the outer ring focused beam are focused onto the same plane to form inner and outer ring focused spots. This plane is the focusing plane of the double-ring focused beam.

[0052] Several immunochromatographic test strips are arranged radially to form a multi-parameter immunochromatographic test strip 11. The plane of the multi-parameter immunochromatographic test strip 11 coincides with the focusing plane. The detection lines 113 of all the immunochromatographic test strips together form an inner ring structure, and the control lines 114 of all the immunochromatographic test strips together form an outer ring structure.

[0053] The sample solution to be tested is loaded onto each immunochromatographic test strip. The inner ring-shaped focused light spot acts on the target analyte fixed on the detection line 113, exciting the corresponding surface Raman scattering; the outer ring-shaped focused light spot acts on the molecular probe fixed on the control line 114, exciting the corresponding surface Raman scattering.

[0054] S3: By performing spectral analysis on the Raman scattered light, the presence of the target analyte and the validity of the immunochromatographic test strip are determined. Specifically, determining the validity of the immunochromatographic test strip involves using spectral analysis to identify the presence of the corresponding molecular probe, thus confirming the strip's effectiveness.

[0055] This invention employs a dual-ring beam design, using dual-ring focused beams to form a high-intensity ring beam on the focal plane. The spatial distribution of this ring beam is geometrically matched to the detection / control lines of the immunochromatographic test strip, achieving linear scanning instead of traditional single-point measurement. This design increases the interaction area between the excitation light and the sample by more than 10 times, effectively improving the excitation efficiency and detection sensitivity of the Raman signal. Simultaneously, the dual-ring beam incident on the target enables simultaneous excitation of multiple targets. The dual-ring beam can simultaneously excite the characteristic Raman peaks of different targets, achieving simultaneous detection of multiple targets with an efficiency improvement of over 80%, significantly enhancing high-throughput multi-index joint detection capabilities.

[0056] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. An optical component capable of forming a double-ring focused beam, characterized in that, It includes a first conical mirror, a second conical mirror, and a third conical mirror, all of which are cones, and a first parabolic cylindrical mirror and a second parabolic cylindrical mirror, both of which are hollow cylinders; the inner surfaces of the first parabolic cylindrical mirror and the second parabolic cylindrical mirror can focus light perpendicular to the axis of the cylinder to the same point respectively; The first, second, and third conical mirrors are arranged coaxially in sequence, with the cone-shaped end of the first conical mirror facing the direction of light incidence, and the cone-shaped ends of the second and third conical mirrors facing opposite directions to the cone-shaped end of the first conical mirror; the first parabolic cylindrical mirror is coaxially fitted outside the first conical mirror, and the second parabolic cylindrical mirror is coaxially fitted outside the first parabolic cylindrical mirror; A flat-top beam is incident on the side of the first conical mirror parallel to the axis of the cone. After being reflected by the mirror, it is incident on the surface of the first parabolic cylindrical mirror in a direction perpendicular to the axis of the cone. The reflected light is incident on the side of the second conical mirror and is reflected by the side of the second conical mirror to form an outer ring focused beam. The light transmitted through the first parabolic cylindrical mirror is incident on the curved surface of the second parabolic cylindrical mirror, and after being reflected by it, it is incident on the side surface of the third conical mirror. The light is reflected by the side surface of the third conical mirror to form an inner ring focused beam. The inner and outer ring focused beams constitute a double ring focused beam.

2. The optical component capable of forming a dual-ring focused beam as described in claim 1, characterized in that, The inner and outer ring-shaped focusing beams are focused onto the same focusing plane, and the double-ring-shaped focusing beam is focused into a double-ring-shaped focusing spot within the focusing plane.

3. The optical component capable of forming a dual-ring focused beam as described in claim 1 or 2, characterized in that, The inner surface of the cylinder is a curved surface formed by rotating a partial parabola around its axis. The directrix of the partial parabola is parallel to the axis of the cylinder, and the focus of the partial parabola is located on the axis of the cylinder.

4. A spectral detection device based on a dual-ring focusing beam, characterized in that, Includes the optical components, laser, shaping module, first filter, stage, spectral analysis module, and multi-parameter immunochromatographic test strip as described in any one of claims 1-3; The laser emits laser light, and the shaping module is used to convert the laser light into a flat-top beam. After the flat-top beam passes through the first filter to filter out stray light and interference light, it is converted into a double-ring focused beam by optical components. The double-ring focused beam forms a double-ring focused spot including inner and outer ring focused spots on the focusing plane. The multi-parameter immunochromatographic test strip consists of several immunochromatographic test strips arranged radially on a stage. The detection lines and control lines on the several immunochromatographic test strips form an inner ring structure and an outer ring structure, respectively. The plane on which the multi-parameter immunochromatographic test strip is located coincides with the focusing plane. The inner and outer ring focusing spots of the dual-ring focusing spot irradiate the inner and outer ring structures, respectively acting on several detection targets fixed on the detection lines and molecular probes fixed on several control lines, and respectively exciting the corresponding Raman scattering light. The Raman scattered light is emitted through the optical components to the first filter to remove interference light, and then emitted to the spectral analysis module for spectral analysis.

5. The spectral detection device based on a dual-ring focusing beam as described in claim 4, characterized in that, The inner surface of the first parabolic cylindrical mirror in the optical component is coated with a beam-splitting film, which makes the light intensity reflected from the inner surface of the first conical mirror less than the light intensity transmitted through the inner surface of the first conical mirror.

6. The spectral detection device based on a dual-ring focusing beam as described in claim 4, characterized in that, The outer surface of the first parabolic cylindrical mirror is coated with an anti-reflective coating.

7. The spectral detection device based on a dual-ring focusing beam as described in claim 4, characterized in that, The second parabolic cylindrical mirror has an anti-reflection coating on its curved surface.

8. The spectral detection device based on a dual-ring focusing beam as described in any one of claims 4-7, characterized in that, The spectral detection device also includes a motor drive mechanism connected to the second conical mirror and the third conical mirror, which is used to drive the second conical mirror and / or the third conical mirror to move along their axial direction.

9. A method for spectral detection based on a dual-ring focused beam, characterized in that, The method is as follows: convert the laser into a flat-top beam and remove stray light; The flat-top beam is converted into a double-ring focused beam, which forms a double-ring focused spot including inner and outer ring focused spots on the focusing plane. The multi-parameter immunochromatographic test strip is designed such that the plane on which the multi-parameter immunochromatographic test strip is located coincides with the focusing plane. The detection line and control line on the multi-parameter immunochromatographic test strip form an inner ring structure and an outer ring structure, respectively. After the multi-parameter immunochromatographic test strip is loaded with a sample, the inner ring focused light spot acts on the target analyte fixed on the detection line, exciting the corresponding surface Raman scattering; The outer ring-shaped focusing spot acts on the molecular probe fixed on the quality control line, exciting the corresponding surface Raman scattering; By performing spectral analysis on the Raman scattered light, it can be determined whether the target analyte is present and whether the immunochromatographic test strip is effective.

10. The dual-ring focused beam spectral detection method as described in claim 9, characterized in that, The flat-top beam is converted into a dual-ring focused beam using the optical components described in any one of claims 1-3.

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