Traditional Chinese medicine composition for improving depression as well as preparation method and application of traditional Chinese medicine composition

Abstract

The invention relates to the technical field of traditional Chinese medicine preparation, in particular to a traditional Chinese medicine composition for improving depression and a preparation method and application thereof, and the traditional Chinese medicine composition comprises the following raw materials: a spina date seed modified extract, cortex albiziae, vinegar-processed modified radix bupleuri, angelica sinensis, radix paeoniae alba, polygala tenuifolia, poria cocos and a functional additive. According to the invention, the spina date seed modified extract can tranquilize mind by nourishing the heart, calm the heart and calm palpitation, and effectively improve symptoms such as insomnia and anxiety accompanied by depression; the vinegar-processed modified radix bupleuri can soothe liver-qi stagnation and smooth qi activity, and plays a key role in liver-qi stagnation of the depression core pathogenesis; the pretreated cortex albiziae can relieve depression, calm the nerves, activate blood, diminish swelling and assist in improving accompanying symptoms such as blood stasis and qi stagnation; the traditional Chinese medicine composition has the advantages that the traditional Chinese medicine composition, the traditional Chinese medicine extract, the radix angelicae sinensis, the radix paeoniae alba and other traditional Chinese medicinal materials have a synergistic effect, the hypothalamus-pituitary-adrenal axis function is improved through multi-target regulation of central neurotransmitters, the improvement effect on depressive symptoms is obviously improved, the compatibility of the medicinal materials is good, the synergistic effect is higher, and the traditional Chinese medicine composition can be widely applied to preparation of medicines or health care products for improving the depressive symptoms.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine preparation technology, specifically to a traditional Chinese medicine composition for improving depression, its preparation method, and its application. Background Technology

[0002] Depression is divided into short-term daily low mood (which can be self-regulated) and depression (persistent low mood and loss of interest that require clinical intervention). The traditional Chinese medicine composition for improving depression is based on the TCM syndrome differentiation and treatment. It works by soothing the liver and relieving depression, nourishing blood and calming the mind, and strengthening the spleen and replenishing qi. It starts by regulating the body's functions to help improve depression-related symptoms such as low mood, insomnia, and fatigue. It should be used under the guidance of a professional TCM doctor and cannot replace the standard clinical treatment of depression.

[0003] In existing technologies, core medicinal materials such as jujube seed and bupleurum are mostly processed using traditional methods, resulting in low dissolution rates of effective components and insufficient compatibility with other components. This leads to limited effectiveness in improving depressive symptoms and fails to fully exert the synergistic effects of nourishing the heart and calming the mind, as well as soothing the liver and relieving depression. Furthermore, there is a lack of functional adjuvants that target component penetration and stability, resulting in low bioavailability of effective components in the body and easy degradation, which affects the sustainability of the efficacy.

[0004] Based on this, the present invention provides a traditional Chinese medicine composition for improving depression, its preparation method and application. Summary of the Invention

[0005] The purpose of this invention is to provide a traditional Chinese medicine composition for improving depression, its preparation method and application. The traditional Chinese medicine composition prepared by this invention not only has a high dissolution rate of active ingredients and a high blood-brain barrier permeability, but also a high retention rate of active ingredients after accelerated testing, that is, high component stability.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a traditional Chinese medicine composition for improving depression, its preparation method and application, comprising the following raw materials in parts by weight: 30-50 parts of modified jujube seed extract, 25-40 parts of Albizia bark, 20-35 parts of vinegar-processed modified Bupleurum root, 15-25 parts of Angelica sinensis, 12-20 parts of Paeonia lactiflora, 10-18 parts of Polygala tenuifolia, 15-20 parts of Poria cocos, and 5-10 parts of functional adjuvants; The functional additive is composed of a synergist, a penetration enhancer, and a stabilizer, with the mass ratio of each raw material being (10-15):(3-5):(2-4). The preparation method of the functional additive is as follows: add the synergistic carrier to the reaction vessel, heat to 50-60℃, stir at 80-100 rpm for 20-30 min, add the penetration promoter and stabilizer and continue stirring for 20-30 min, spray dry the obtained product, pulverize it through a 180-200 mesh sieve to obtain the functional additive.

[0007] Preferably, the synergistic carrier is a modified inulin, the penetration enhancer is menthol, the stabilizer is vitamin E, and the inlet air temperature of the spray dryer is 160-180℃, and the outlet air temperature is 80-90℃.

[0008] Preferably, the specific preparation steps of the modified jujube seed extract are as follows: Jujube seed powder obtained by rinsing with deionized water and pulverizing and sieving is added to an ethanol aqueous solution, then placed in a constant temperature water bath, and the temperature is controlled at 50-60℃ for 1-2 hours. During this period, the mixture is stirred every 20 minutes at a stirring speed of 100-150 rpm. After extraction, the residue is collected by filtration. The residue is added to the modification solution and stirred in a constant temperature water bath at 70-80℃ at a stirring speed of 120-150 rpm for 1-1.5 hours. Then, centrifugation is performed to collect the precipitate of the acid-modified active ingredients. The precipitate is dried at 105-115℃ to constant weight, pulverized, and passed through a 150-200 mesh sieve. The sieve-passing material is collected to obtain the modified jujube seed extract.

[0009] Preferably, the mass of the added ethanol aqueous solution is 3-5 times that of the crude jujube seed powder, and the volume fraction of ethanol is 60-70%; the modified solution is prepared by mixing glacial acetic acid and deionized water at a mass ratio of 2:1, the pH is 2.0-2.5, and the solid-liquid ratio of the filter residue to the modified solution is 1:(8-10).

[0010] Preferably, the preparation method of the vinegar-modified Bupleurum is as follows: Bupleurum root is washed with deionized water and sliced ​​to obtain raw Bupleurum slices; the raw Bupleurum slices are added to vinegar solution, mixed well and placed in a sealed container, and moistened in a constant temperature environment of 25-30℃ for 4-6 hours, turning once every 1 hour during this period; the moistened Bupleurum slices are stir-fried at 150-160℃ for 5-8 minutes, until the surface of the Bupleurum slices turns yellowish-brown, a strong roasted aroma is emitted and there are no burnt spots, and then they are immediately removed; finally, they are pulverized and passed through a 100-120 mesh sieve to obtain the sieve-less material, thus obtaining vinegar-modified Bupleurum.

[0011] Preferably, the vinegar solution is prepared by mixing rice vinegar and deionized water at a mass ratio of 1:5 and stirring, and the pH is 3.0-3.5, and the solid-liquid ratio of Bupleurum root slices to vinegar solution is 1:(3-4).

[0012] Preferably, the preparation steps of the inulin-modified product are as follows: Step 1: Preparation of 6-bromo-6-deoxyinulin: Inulin was dispersed in N,N-dimethylformamide, N-bromosuccinimide was added, the temperature was lowered to 0℃, and a triphenylphosphine solution dissolved in N,N-dimethylformamide was slowly added dropwise. After the addition was completed, the reaction was carried out in an ice-water bath under nitrogen protection for 30 min, and then the temperature was raised to 80℃ and the reaction was continued for 3 h. The reaction solution was poured into 5 times the volume of acetone, and the precipitate was allowed to stand for 2 h. After filtration, the precipitate was washed 3 times with acetone and lyophilized to obtain the product. Step 2, Preparation of hydrophobic 6-bromo-6-deoxyinulin: Dissolve the product from Step 1 in anhydrous pyridine, add acetic anhydride, react at room temperature for 12 h under nitrogen protection, pour the reaction solution into an ice-water bath and stir to precipitate, filter, wash with deionized water until neutral, and freeze dry to obtain the product. Step 3, Preparation of 6-azido-6-deoxyinulin: Dissolve the product from Step 2 in N,N-dimethylformamide, add sodium azide, stir and react at 80°C for 4 hours under nitrogen protection, pour the reaction solution into 3 times the volume of ice water, let it stand to precipitate for 1 hour, filter and wash the precipitate twice with ice water, freeze dry to obtain the product. Step 4, Preparation of 6-amino-6-deoxyinulin: Dissolve the product from step 3 in N,N-dimethylformamide, add triphenylphosphine, react at 60℃ for 12 h, add a 3:1 volume ratio of diethyl ether-ethanol mixed solution to precipitate, filter, wash the precipitate 3 times with anhydrous ethanol, and freeze-dry to obtain the inulin-modified product.

[0013] Preferably, the molar ratio of inulin to N-bromosuccinimide is 1:1.2, the molar ratio of triphenylphosphine to N-bromosuccinimide is 1:1, the molar ratio of the product obtained in step one to acetic anhydride is 1:3, the molar ratio of the product obtained in step two to sodium azide is 1:1.5, and the molar ratio of the product in step three to triphenylphosphine is 1:1.3.

[0014] A method for preparing a traditional Chinese medicine composition for improving depression includes the following steps: S1: Grind Angelica sinensis, Paeonia lactiflora, Polygala tenuifolia, and Poria cocos into powder, pass them through a 100-120 mesh sieve, and obtain fine powder of each herb for later use; S2: Take Albizia bark, remove impurities and cut it into small sections of 2-3cm. Add 5 times its weight of a decoction prepared by boiling licorice and deionized water in a mass ratio of 1:20 for 30 minutes and then filtering. Boil at 85℃ for 1.2 hours, filter and take the residue. Drain at room temperature and then crush to 110 mesh to obtain pretreated Albizia bark. S3: Mix the modified jujube seed extract, vinegar-modified Bupleurum chinense, pretreated Albizia julibrissin bark, and the fine powder of each single herb obtained in step S1 evenly. Add 6 times the total mass of the mixed powder to deionized water, soak at 65℃ for 35 minutes, then heat to 95℃ and decoct for 1.8 hours. Filter with a 200-mesh filter cloth and collect the first filtrate. Add 4 times the mass of the residue to deionized water, decoct at 95℃ for 1.2 hours, filter and collect the second filtrate, and combine the two filtrates. S4: Place the combined filtrate in a vacuum concentration tank, control the vacuum degree to -0.085MPa and the temperature to 65℃, and concentrate under reduced pressure to a clear paste with a relative density of 1.18; S5: Add the prescribed amount of functional adjuvant to the extract, stir at 55℃ and 90rpm for 30 minutes until the mixture is uniform, then feed it into a granulator to granulate. The granules are dried at 60℃ and sieved through an 18-mesh sieve to obtain the traditional Chinese medicine composition.

[0015] Application of a traditional Chinese medicine composition for improving depression, wherein the traditional Chinese medicine composition is used in the preparation of drugs or health products for improving depressive symptoms, wherein the depressive symptoms include low mood, insomnia and dreaminess, anxiety and irritability, chest tightness and rib distension, and difficulty concentrating.

[0016] Compared with the prior art, the beneficial effects of the present invention are: 1. This invention uses a modified jujube seed extract, which, after extraction with ethanol aqueous solution and treatment with acidic modification solution, significantly improves the dissolution rate of effective components and enhances compatibility with raw materials such as angelica and white peony. It can work synergistically with vinegar-processed modified bupleurum to nourish the heart and calm the mind, improving insomnia and anxiety associated with depression. On the other hand, it lays the foundation for calming the mind and soothing the liver and relieving depression with vinegar-processed modified bupleurum. The two work together to cover the core syndrome of depression, greatly improving the effect on symptoms such as low mood and sleep disorders.

[0017] 2. This invention uses vinegar-modified Bupleurum chinense. After being moistened in vinegar and stir-fried at high temperature, the stability of the active ingredients is improved and the liver-targeting ability is enhanced. It can work synergistically with the modified Ziziphus jujuba var. spinosa extract to not only relieve the root cause of emotional depression by soothing the liver and relieving depression, but also promote the absorption and utilization of the active ingredients of Ziziphus jujuba var. spinosa. At the same time, it works synergistically with Albizia julibrissin bark, Polygala tenuifolia, etc. to regulate Qi, invigorate blood and calm the mind, and improve the symptoms of depression such as chest tightness, hypochondriac distension and emotional depression caused by liver Qi stagnation at multiple targets.

[0018] 3. In this invention, inulin-modified material is used as a synergistic carrier for functional adjuvants. Its amino groups can form stable bonds with the active ingredients in raw materials such as modified jujube seed extract and vinegar-modified Bupleurum chinense. At the same time, it works synergistically with the penetration enhancer menthol and the stabilizer vitamin E to improve the permeability and stability of the active ingredients, making the synergistic effect of each Chinese herbal raw material more lasting and efficient, and significantly enhancing the overall efficacy of the composition in improving depressive symptoms. Detailed Implementation

[0019] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0020] To enable those skilled in the art to better understand the present application, the technical solutions in the embodiments of the present application are described clearly and completely.

[0021] Example 1: A traditional Chinese medicine composition for improving depression, comprising the following raw materials in parts by weight: 30 parts modified jujube seed extract, 25 parts Albizia bark, 20 parts vinegar-processed modified Bupleurum root, 15 parts Angelica sinensis, 12 parts Paeonia lactiflora, 10 parts Polygala tenuifolia, 15 parts Poria cocos, and 5 parts functional adjuvants. Angelica sinensis and white peony root nourish blood and soften the liver, working together with Bupleurum chinense to relieve liver stagnation and blood deficiency. Polygala tenuifolia and Poria cocos calm the mind, improve intelligence, strengthen the spleen and eliminate dampness, enhancing the overall calming and mood-lifting effects. The functional additives consist of a synergist, a penetration enhancer, and a stabilizer, with a mass ratio of 10:3:2. The preparation method of the functional additive is as follows: the synergist carrier is added to the reaction vessel, heated to 50°C, stirred at 80 rpm for 20 min, the penetration promoter and stabilizer are added and stirred for another 20 min, the resulting product is spray dried and then pulverized through a 180-mesh sieve to obtain the functional additive. The synergistic carrier is inulin-modified compound, the penetration enhancer is menthol to improve the bioavailability of the ingredients, and the stabilizer is vitamin E to prevent the degradation of the active ingredients during preparation and storage. The inlet air temperature of the spray dryer is 160℃ and the outlet air temperature is 80℃. The modified jujube seed extract and vinegar-processed modified bupleurum nourish the heart and soothe the liver, treating both the mind and liver qi simultaneously, covering the core pathogenesis of depression and improving both mood and sleep symptoms; vinegar-processed bupleurum, angelica, and white peony soothe the liver and regulate qi, while nourishing blood and softening the liver, regulating liver stagnation and blood deficiency, relieving chest tightness, hypochondriac distension, and low mood; albizia bark, polygala, and poria relieve depression and calm the mind, while strengthening the spleen and calming the mind, enhancing the sedative and calming effects, relieving anxiety and poor concentration; modified inulin, menthol, and vitamin E improve the overall blood-brain barrier permeability and component stability of the formulation.

[0022] The specific preparation steps of the modified jujube seed extract are as follows: Step 1: Raw material pretreatment: Select high-quality jujube seed medicinal material that is free from mold and insect infestation, remove impurities and residual fruit shells, rinse quickly 3 times with deionized water, dry in a forced-air dryer at 60℃ for 2 hours, cool to room temperature, and then pulverize to 80 mesh to obtain coarse jujube seed powder. Step 2, Degreasing and impurity removal: Add the coarse jujube seed powder to the ethanol aqueous solution, then place it in a constant temperature water bath, control the temperature at 50℃, and extract at a constant temperature for 1 hour. During this period, stir once every 20 minutes at a stirring speed of 100 rpm (5 minutes each time). After extraction, filter with a 200-mesh filter cloth and collect the filter residue. Step 3, Acid Modification: Add the filter residue to the modification solution and stir in a 70℃ constant temperature water bath for 1 hour at a stirring speed of 120 rpm to ensure that the filter residue is fully dispersed in the modification solution; Step 4: Separation and purification: Transfer the modified mixture into a centrifuge tube, centrifuge at 8000 rpm for 15 min, discard the supernatant, and collect the precipitate of the acid-modified active ingredient. Step 5, Drying and pulverizing: Place the washed precipitate in a constant temperature drying oven at 105℃, dry it to constant weight, cool it to room temperature, pulverize it to 150 mesh using an ultra-micro pulverizer, sieve it, seal and store it to obtain the modified jujube seed extract. Modified jujube seed extract can enhance the effects of nourishing the heart and calming the mind, effectively improving symptoms such as insomnia and anxiety associated with depression.

[0023] The mass of the added ethanol-water solution was three times that of the crude jujube seed powder, and the volume fraction of ethanol was 60%. The modified solution was prepared by mixing glacial acetic acid and deionized water at a mass ratio of 2:1, with a pH of 2.0, and the solid-liquid ratio of the filter residue to the modified solution was 1:8.

[0024] The preparation method of vinegar-modified Bupleurum is as follows: Step 1: Raw material screening and pretreatment: Select Bupleurum root that is free from rot, mold, and insect infestation, remove fibrous roots, mud and impurities, rinse 3 times with deionized water, drain the surface moisture, place in a 60℃ forced-air drying oven to dry for 3 hours, cool to room temperature, and then cut into uniform thin slices with a thickness of 0.3cm using a slicer to obtain raw Bupleurum slices. Step 2, Vinegar preparation and humidification modification: Add Bupleurum root slices to vinegar solution, stir well, transfer to a sealed container, and place in a constant temperature environment of 25℃ for humidification for 4 hours. During this period, stir once every 1 hour (3 minutes each time) to ensure that the vinegar solution is evenly absorbed by the Bupleurum root slices until there is no obvious free vinegar solution residue. Step 3, Stir-frying and solidification: Preheat the stir-frying machine to 120℃, put in the moistened Bupleurum slices, control the stir-frying speed to 30r / min, stir-fry at a low temperature for 2 minutes, then raise the temperature to 150℃ and continue to stir-fry for 3 minutes, until the surface of the Bupleurum slices turns yellowish-brown, a strong roasted aroma is emitted and there are no burnt spots, then take it out immediately. Step 4, Cooling and Grinding: Quickly spread the stir-fried Bupleurum slices on a clean porcelain plate and place them in a cool and ventilated place to cool to room temperature. Grind them with a universal grinder, pass them through a 100-mesh sieve, collect the sieve material, seal it and store it in a dry environment to obtain vinegar-modified Bupleurum. Vinegar-processed modified Bupleurum enhances its effects of soothing the liver, relieving depression, and regulating Qi, playing a key therapeutic role in addressing the core pathogenesis of depression—liver Qi stagnation.

[0025] The vinegar solution was prepared by mixing rice vinegar and deionized water at a mass ratio of 1:5 and stirring, with a pH of 3.0. The solid-liquid ratio of Bupleurum root slices to the vinegar solution was 1:3.

[0026] The preparation steps of inulin-modified products are as follows: Step 1: Preparation of 6-bromo-6-deoxyinulin: Inulin was dispersed in N,N-dimethylformamide, N-bromosuccinimide was added, the temperature was lowered to 0℃, and a triphenylphosphine solution dissolved in N,N-dimethylformamide was slowly added dropwise. After the addition was completed, the reaction was carried out in an ice-water bath under nitrogen protection for 30 min, and then the temperature was raised to 80℃ and the reaction was continued for 3 h. The reaction solution was poured into 5 times the volume of acetone, and the precipitate was allowed to stand for 2 h. After filtration, the precipitate was washed 3 times with acetone and lyophilized to obtain the product. Step 2, Preparation of hydrophobic 6-bromo-6-deoxyinulin: Dissolve the product from Step 1 in anhydrous pyridine, add acetic anhydride, react at room temperature for 12 h under nitrogen protection, pour the reaction solution into an ice-water bath and stir to precipitate, filter, wash with deionized water until neutral, and freeze dry to obtain the product. Step 3, Preparation of 6-azido-6-deoxyinulin: Dissolve the product from Step 2 in N,N-dimethylformamide, add sodium azide, stir and react at 80°C for 4 hours under nitrogen protection, pour the reaction solution into 3 times the volume of ice water, let it stand to precipitate for 1 hour, filter and wash the precipitate twice with ice water, freeze dry to obtain the product. Step 4, Preparation of 6-amino-6-deoxyinulin: Dissolve the product of step 3 in N,N-dimethylformamide, add triphenylphosphine, react at 60℃ for 12h, add a 3:1 volume ratio of diethyl ether-ethanol mixed solution to precipitate, filter, wash the precipitate 3 times with anhydrous ethanol, and freeze-dry to obtain inulin-modified product. Inulin-modified compounds, as synergistic carriers, improve the stability of the active ingredients in the composition and the blood-brain barrier permeability.

[0027] The molar ratio of inulin to N-bromosuccinimide is 1:1.2, and the molar ratio of triphenylphosphine to N-bromosuccinimide is 1:1; the molar ratio of the product obtained in step one to acetic anhydride is 1:3; the molar ratio of the product obtained in step two to sodium azide is 1:1.5; and the molar ratio of the product in step three to triphenylphosphine is 1:1.3.

[0028] A method for preparing a traditional Chinese medicine composition for improving depression includes the following steps: S1: Grind Angelica sinensis, Paeonia lactiflora, Polygala tenuifolia, and Poria cocos into powder, pass them through a 100-mesh sieve, and obtain fine powder of each herb for later use; S2: Take Albizia bark, remove impurities and cut it into 2cm pieces. Add 5 times its weight of a decoction made by boiling licorice and deionized water in a mass ratio of 1:20 for 30 minutes and then filtering. Boil at 85℃ for 1.2 hours, filter and take the residue. Drain at room temperature and then crush to 110 mesh to obtain pretreated Albizia bark. S3: Mix the modified jujube seed extract, vinegar-modified Bupleurum chinense, pretreated Albizia julibrissin bark, and the fine powder of each single herb obtained in step S1 evenly. Add 6 times the total mass of the mixed powder to deionized water, soak at 65℃ for 35 minutes, then heat to 95℃ and decoct for 1.8 hours. Filter with a 200-mesh filter cloth and collect the first filtrate. Add 4 times the mass of the residue to deionized water, decoct at 95℃ for 1.2 hours, filter and collect the second filtrate, and combine the two filtrates. S4: Place the combined filtrate in a vacuum concentration tank, control the vacuum degree to -0.085MPa and the temperature to 65℃, and concentrate under reduced pressure to a clear paste with a relative density of 1.18; S5: Add the prescribed amount of functional adjuvant to the extract, stir at 55℃ and 90rpm for 30 minutes until the mixture is uniform, then feed it into a granulator to granulate. The granules are dried at 60℃ and sieved through an 18-mesh sieve to obtain the traditional Chinese medicine composition.

[0029] Application of traditional Chinese medicine compositions for improving depression, and application of traditional Chinese medicine compositions in the preparation of drugs or health products for improving depressive symptoms, including low mood, insomnia and dreaminess, anxiety and irritability, chest tightness and rib distension, and poor concentration.

[0030] Example 2: A traditional Chinese medicine composition for improving depression, comprising the following raw materials in parts by weight: 40 parts modified jujube seed extract, 32 parts Albizia bark, 27 parts vinegar-processed modified Bupleurum root, 20 parts Angelica sinensis, 16 parts Paeonia lactiflora, 14 parts Polygala tenuifolia, 17 parts Poria cocos, and 7 parts functional adjuvants. The functional additives consist of a synergist carrier, a penetration enhancer, and a stabilizer, with a mass ratio of 12:4:3. The preparation method of the functional additive is as follows: the synergist carrier is added to the reaction vessel, heated to 55°C, stirred at 90 rpm for 25 min, the penetration promoter and stabilizer are added and stirred for another 25 min, the resulting product is spray-dried and then pulverized through a 190-mesh sieve to obtain the functional additive.

[0031] The synergistic carrier is inulin-modified material, the penetration enhancer is menthol, the stabilizer is vitamin E, and the inlet air temperature for spray drying is 170℃ and the outlet air temperature is 85℃.

[0032] The specific preparation steps of the modified jujube seed extract are as follows: Step 1: Raw material pretreatment: Select high-quality jujube seed medicinal material that is free from mold and insect infestation, remove impurities and residual fruit shells, rinse quickly 3 times with deionized water, dry in a forced-air dryer at 65℃ for 2.5 hours, cool to room temperature, and then pulverize to 90 mesh to obtain coarse jujube seed powder. Step 2, Degreasing and impurity removal: Add the coarse jujube seed powder to the ethanol aqueous solution, then place it in a constant temperature water bath, control the temperature at 55℃, and extract at a constant temperature for 1.5 hours. During this period, stir once every 20 minutes at a stirring speed of 125 rpm (5 minutes each time). After extraction, filter with a 200-mesh filter cloth and collect the filter residue. Step 3, Acid Modification: Add the filter residue to the modification solution and stir in a 75℃ constant temperature water bath for 1.2 hours at a stirring speed of 135 rpm to ensure that the filter residue is fully dispersed in the modification solution; Step 4: Separation and purification: Transfer the modified mixture into a centrifuge tube, centrifuge at 9000 rpm for 17 min, discard the supernatant, and collect the precipitate of the acid-modified active ingredient. Step 5: Drying and pulverizing: Place the washed precipitate in a constant temperature drying oven at 110℃ and dry it to constant weight. After cooling to room temperature, pulverize it to 175 mesh using an ultrafine pulverizer, sieve it, and seal it for storage to obtain the modified jujube seed extract.

[0033] The mass of the added ethanol-water solution was 4 times that of the crude jujube seed powder, and the volume fraction of ethanol was 65%. The modified solution was prepared by mixing glacial acetic acid and deionized water at a mass ratio of 2:1, with a pH of 2.2, and the solid-liquid ratio of the filter residue to the modified solution was 1:9.

[0034] The preparation method of vinegar-modified Bupleurum is as follows: Step 1: Raw material screening and pretreatment: Select Bupleurum root that is free from rot, mold, and insect infestation, remove fibrous roots, mud and impurities, rinse 3 times with deionized water, drain the surface moisture, place in a 62℃ forced-air drying oven to dry for 3.5 hours, cool to room temperature, and then cut into uniform thin slices with a thickness of 0.4 cm using a slicer to obtain raw Bupleurum slices. Step 2, Vinegar preparation and humidification modification: Add Bupleurum root slices to vinegar solution, stir well, transfer to a sealed container, and place in a constant temperature environment of 27℃ for humidification for 5 hours. During this period, stir once every 1 hour (3 minutes each time) to ensure that the vinegar solution is evenly absorbed by the Bupleurum root slices until there is no obvious free vinegar solution residue. Step 3, Stir-frying and solidification: Preheat the stir-frying machine to 125℃, put in the moistened Bupleurum slices, control the stir-frying speed to 35r / min, stir-fry at a low temperature for 2 minutes, then raise the temperature to 155℃ and continue to stir-fry for 4 minutes, until the surface of the Bupleurum slices turns yellowish-brown, a strong roasted aroma is emitted and there are no burnt spots, then take it out immediately. Step 4: Cooling and Grinding: Quickly spread the stir-fried Bupleurum slices on a clean porcelain plate and place them in a cool, ventilated place to cool to room temperature. Grind them with a universal grinder, pass them through a 110-mesh sieve, collect the sieve material, seal it and store it in a dry environment to obtain vinegar-modified Bupleurum.

[0035] The vinegar solution was prepared by mixing rice vinegar and deionized water at a mass ratio of 1:5 and stirring, with a pH of 3.2. The solid-liquid ratio of Bupleurum root slices to the vinegar solution was 1:3.5.

[0036] The preparation steps of inulin-modified products are as follows: Step 1: Preparation of 6-bromo-6-deoxyinulin: Inulin was dispersed in N,N-dimethylformamide, N-bromosuccinimide was added, the temperature was lowered to 0℃, and a triphenylphosphine solution dissolved in N,N-dimethylformamide was slowly added dropwise. After the addition was completed, the reaction was carried out in an ice-water bath under nitrogen protection for 30 min, and then the temperature was raised to 80℃ and the reaction was continued for 3 h. The reaction solution was poured into 5 times the volume of acetone, and the precipitate was allowed to stand for 2 h. After filtration, the precipitate was washed 3 times with acetone and lyophilized to obtain the product. Step 2, Preparation of hydrophobic 6-bromo-6-deoxyinulin: Dissolve the product from Step 1 in anhydrous pyridine, add acetic anhydride, react at room temperature for 12 h under nitrogen protection, pour the reaction solution into an ice-water bath and stir to precipitate, filter, wash with deionized water until neutral, and freeze dry to obtain the product. Step 3, Preparation of 6-azido-6-deoxyinulin: Dissolve the product from Step 2 in N,N-dimethylformamide, add sodium azide, stir and react at 80°C for 4 hours under nitrogen protection, pour the reaction solution into 3 times the volume of ice water, let it stand to precipitate for 1 hour, filter and wash the precipitate twice with ice water, freeze dry to obtain the product. Step 4, Preparation of 6-amino-6-deoxyinulin: Dissolve the product from step 3 in N,N-dimethylformamide, add triphenylphosphine, react at 60℃ for 12 h, add a 3:1 volume ratio of diethyl ether-ethanol mixed solution to precipitate, filter, wash the precipitate 3 times with anhydrous ethanol, and freeze-dry to obtain the inulin-modified product.

[0037] The molar ratio of inulin to N-bromosuccinimide is 1:1.2, and the molar ratio of triphenylphosphine to N-bromosuccinimide is 1:1; the molar ratio of the product obtained in step one to acetic anhydride is 1:3; the molar ratio of the product obtained in step two to sodium azide is 1:1.5; and the molar ratio of the product in step three to triphenylphosphine is 1:1.3.

[0038] A method for preparing a traditional Chinese medicine composition for improving depression includes the following steps: S1: Grind Angelica sinensis, Paeonia lactiflora, Polygala tenuifolia, and Poria cocos into powder, pass them through a 110-mesh sieve, and obtain fine powder of each herb for later use; S2: Take Albizia bark, remove impurities and cut it into 2.5cm pieces. Add 5 times its weight of a decoction made by boiling licorice and deionized water in a mass ratio of 1:20 for 30 minutes and then filtering. Boil at 85℃ for 1.2 hours, filter, take the residue, drain at room temperature and crush to 110 mesh to obtain pretreated Albizia bark. S3: Mix the modified jujube seed extract, vinegar-modified Bupleurum chinense, pretreated Albizia julibrissin bark, and the fine powder of each single herb obtained in step S1 evenly. Add 6 times the total mass of the mixed powder to deionized water, soak at 65℃ for 35 minutes, then heat to 95℃ and decoct for 1.8 hours. Filter with a 200-mesh filter cloth and collect the first filtrate. Add 4 times the mass of the residue to deionized water, decoct at 95℃ for 1.2 hours, filter and collect the second filtrate, and combine the two filtrates. S4: Place the combined filtrate in a vacuum concentration tank, control the vacuum degree to -0.085MPa and the temperature to 65℃, and concentrate under reduced pressure to a clear paste with a relative density of 1.18; S5: Add the prescribed amount of functional adjuvant to the extract, stir at 55℃ and 90rpm for 30 minutes until the mixture is uniform, then feed it into a granulator to granulate. The granules are dried at 60℃ and sieved through an 18-mesh sieve to obtain the traditional Chinese medicine composition.

[0039] Application of traditional Chinese medicine compositions for improving depression, and application of traditional Chinese medicine compositions in the preparation of drugs or health products for improving depressive symptoms, including low mood, insomnia and dreaminess, anxiety and irritability, chest tightness and rib distension, and poor concentration.

[0040] Example 3: A traditional Chinese medicine composition for improving depression, comprising the following raw materials in parts by weight: 50 parts modified jujube seed extract, 40 parts Albizia bark, 35 parts vinegar-processed modified Bupleurum root, 25 parts Angelica sinensis, 20 parts Paeonia lactiflora, 18 parts Polygala tenuifolia, 20 parts Poria cocos, and 10 parts functional adjuvants. The functional additives consist of a synergist carrier, a penetration enhancer, and a stabilizer, with a mass ratio of 15:5:4. The preparation method of the functional additive is as follows: the synergist carrier is added to the reaction vessel, heated to 60°C, stirred at 100 rpm for 30 min, the penetration promoter and stabilizer are added and stirred for another 30 min, the resulting product is spray-dried and then pulverized through a 200-mesh sieve to obtain the functional additive.

[0041] The synergistic carrier is inulin-modified material, the penetration enhancer is menthol, the stabilizer is vitamin E, and the inlet air temperature for spray drying is 180℃ and the outlet air temperature is 90℃.

[0042] The specific preparation steps of the modified jujube seed extract are as follows: Step 1: Raw material pretreatment: Select high-quality jujube seed medicinal material that is free from mold and insect infestation, remove impurities and residual fruit shells, rinse quickly 3 times with deionized water, dry in a forced-air dryer at 70℃ for 3 hours, cool to room temperature, and then grind to 120 mesh to obtain jujube seed coarse powder. Step 2, Degreasing and impurity removal: Add the coarse jujube seed powder to the ethanol aqueous solution, then place it in a constant temperature water bath, control the temperature at 60℃, and extract at a constant temperature for 2 hours. During this period, stir once every 20 minutes at a stirring speed of 150 rpm (5 minutes each time). After extraction, filter with a 200-mesh filter cloth and collect the filter residue. Step 3, Acid Modification: Add the filter residue to the modification solution and stir in an 80℃ constant temperature water bath for 1.5 hours at a stirring speed of 150 rpm to ensure that the filter residue is fully dispersed in the modification solution; Step 4: Separation and purification: Transfer the modified mixture into a centrifuge tube, centrifuge at 10,000 rpm for 20 min, discard the supernatant, and collect the precipitate of the acid-modified active ingredient. Step 5: Drying and pulverizing: Place the washed precipitate in a constant temperature drying oven at 115℃ and dry it to constant weight. After cooling to room temperature, pulverize it to 200 mesh using an ultrafine pulverizer, sieve it, and seal it for storage to obtain the modified jujube seed extract.

[0043] The mass of the added ethanol-water solution was 5 times that of the crude jujube seed powder, and the volume fraction of ethanol was 70%. The modified solution was prepared by mixing glacial acetic acid and deionized water at a mass ratio of 2:1, with a pH of 2.5, and the solid-liquid ratio of the filter residue to the modified solution was 1:10.

[0044] The preparation method of vinegar-modified Bupleurum is as follows: Step 1: Raw material screening and pretreatment: Select Bupleurum root that is free from rot, mold, and insect infestation, remove fibrous roots, mud and impurities, rinse 3 times with deionized water, drain the surface moisture, place in a 65℃ forced-air drying oven to dry for 4 hours, cool to room temperature, and then cut into uniform thin slices with a thickness of 0.5cm using a slicer to obtain raw Bupleurum slices. Step 2, Vinegar preparation and humidification modification: Add Bupleurum root slices to vinegar solution, stir well, transfer to a sealed container, and place in a constant temperature environment of 30℃ for humidification for 6 hours. During this period, stir once every 1 hour (3 minutes each time) to ensure that the vinegar solution is evenly absorbed by the Bupleurum root slices until there is no obvious free vinegar solution residue. Step 3, Stir-frying and solidification: Preheat the stir-frying machine to 130℃, put in the moistened Bupleurum slices, control the stir-frying speed to 40r / min, stir-fry at a low temperature for 3 minutes, then raise the temperature to 160℃ and continue to stir-fry for 5 minutes, until the surface of the Bupleurum slices turns yellowish-brown, a strong roasted aroma is emitted and there are no burnt spots, then take it out immediately. Step 4, Cooling and Grinding: Quickly spread the stir-fried Bupleurum slices on a clean porcelain plate and place them in a cool, ventilated place to cool to room temperature. Grind them with a universal grinder, pass them through a 120-mesh sieve, collect the sieve material, seal it and store it in a dry environment to obtain vinegar-modified Bupleurum.

[0045] The vinegar solution was prepared by mixing rice vinegar and deionized water at a mass ratio of 1:5 and stirring, with a pH of 3.5. The solid-liquid ratio of Bupleurum root slices to the vinegar solution was 1:4.

[0046] The preparation steps of inulin-modified products are as follows: Step 1: Preparation of 6-bromo-6-deoxyinulin: Inulin was dispersed in N,N-dimethylformamide, N-bromosuccinimide was added, the temperature was lowered to 0℃, and a triphenylphosphine solution dissolved in N,N-dimethylformamide was slowly added dropwise. After the addition was completed, the reaction was carried out in an ice-water bath under nitrogen protection for 30 min, and then the temperature was raised to 80℃ and the reaction was continued for 3 h. The reaction solution was poured into 5 times the volume of acetone, and the precipitate was allowed to stand for 2 h. After filtration, the precipitate was washed 3 times with acetone and lyophilized to obtain the product. Step 2, Preparation of hydrophobic 6-bromo-6-deoxyinulin: Dissolve the product from Step 1 in anhydrous pyridine, add acetic anhydride, react at room temperature for 12 h under nitrogen protection, pour the reaction solution into an ice-water bath and stir to precipitate, filter, wash with deionized water until neutral, and freeze dry to obtain the product. Step 3, Preparation of 6-azido-6-deoxyinulin: Dissolve the product from Step 2 in N,N-dimethylformamide, add sodium azide, stir and react at 80°C for 4 hours under nitrogen protection, pour the reaction solution into 3 times the volume of ice water, let it stand to precipitate for 1 hour, filter and wash the precipitate twice with ice water, freeze dry to obtain the product. Step 4, Preparation of 6-amino-6-deoxyinulin: Dissolve the product from step 3 in N,N-dimethylformamide, add triphenylphosphine, react at 60℃ for 12 h, add a 3:1 volume ratio of diethyl ether-ethanol mixed solution to precipitate, filter, wash the precipitate 3 times with anhydrous ethanol, and freeze-dry to obtain the inulin-modified product.

[0047] The molar ratio of inulin to N-bromosuccinimide is 1:1.2, and the molar ratio of triphenylphosphine to N-bromosuccinimide is 1:1; the molar ratio of the product obtained in step one to acetic anhydride is 1:3; the molar ratio of the product obtained in step two to sodium azide is 1:1.5; and the molar ratio of the product in step three to triphenylphosphine is 1:1.3.

[0048] A method for preparing a traditional Chinese medicine composition for improving depression includes the following steps: S1: Grind Angelica sinensis, Paeonia lactiflora, Polygala tenuifolia, and Poria cocos into powder, pass them through a 120-mesh sieve, and obtain fine powder of each herb for later use; S2: Take Albizia bark, remove impurities and cut it into 3cm pieces. Add 5 times its weight of a decoction made by boiling licorice and deionized water in a mass ratio of 1:20 for 30 minutes and then filtering. Boil at 85℃ for 1.2 hours, filter and take the residue. Drain at room temperature and then crush to 110 mesh to obtain pretreated Albizia bark. S3: Mix the modified jujube seed extract, vinegar-modified Bupleurum chinense, pretreated Albizia julibrissin bark, and the fine powder of each single herb obtained in step S1 evenly. Add 6 times the total mass of the mixed powder to deionized water, soak at 65℃ for 35 minutes, then heat to 95℃ and decoct for 1.8 hours. Filter with a 200-mesh filter cloth and collect the first filtrate. Add 4 times the mass of the residue to deionized water, decoct at 95℃ for 1.2 hours, filter and collect the second filtrate, and combine the two filtrates. S4: Place the combined filtrate in a vacuum concentration tank, control the vacuum degree to -0.085MPa and the temperature to 65℃, and concentrate under reduced pressure to a clear paste with a relative density of 1.18; S5: Add the prescribed amount of functional adjuvant to the extract, stir at 55℃ and 90rpm for 30 minutes until the mixture is uniform, then feed it into a granulator to granulate. The granules are dried at 60℃ and sieved through an 18-mesh sieve to obtain the traditional Chinese medicine composition.

[0049] Application of traditional Chinese medicine compositions for improving depression, and application of traditional Chinese medicine compositions in the preparation of drugs or health products for improving depressive symptoms, including low mood, insomnia and dreaminess, anxiety and irritability, chest tightness and rib distension, and poor concentration.

[0050] Comparative Example 1: The difference between this comparative example and Example 1 is that commercially available jujube kernels are used in this comparative example.

[0051] Comparative Example 2: The difference between this comparative example and Example 1 is that commercially available Bupleurum chinense is used in this comparative example.

[0052] Comparative Example 3 differs from Example 1 in that commercially available inulin is used in this comparative example.

[0053] Performance testing: Performance tests were conducted on the products prepared in Examples 1, 2, 3, Comparative Examples 1, 2, and 3. Dissolution rate test of active ingredient: First, prepare the test solution (take the particles of the composition into the dissolution apparatus, add simulated gastrointestinal fluid, take samples at specific temperatures and speeds and filter them) and the mixed reference solution (accurately weigh each reference standard, dissolve and dilute with methanol); then set the chromatographic conditions such as octadecylsilane bonded silica column, gradient elution mobile phase, and corresponding detection wavelength, and inject the two solutions into the liquid chromatograph to determine the peak area; finally, calculate the amount of active ingredient dissolved at each time point according to the external standard method, and obtain the dissolution rate by the ratio of the amount dissolved to the total content. Test standard: GB / T16631-2008; Blood-brain barrier permeability test: An in vitro blood-brain barrier model co-cultured with rat brain microvascular endothelial cells and astrocytes was constructed, and the effectiveness of the model was verified by transepithelial electrical resistance measurement. Subsequently, the test sample and control solution were prepared and labeled according to the standard. The test sample was added to the upper chamber of the Transwell and the blank culture medium was added to the lower chamber. Samples were taken and replenished at regular intervals under 37℃ and 5% CO2 conditions. The collected samples were temporarily stored at 4℃ and HPLC detection was completed within 2 hours. Finally, the permeability was calculated according to the formula, and the results were verified by three parallel determinations. The test was conducted according to ISO15189:2022. Stability testing of components: First, the granules of the traditional Chinese medicine composition in the examples / comparative examples were divided into three portions according to the simulated market packaging, and a mixed reference solution of jujuboside A and B and bupleuronide a and d was prepared. Then, the packaged test samples were placed in a stability test chamber at 40℃±2℃ and 75%±5% relative humidity and stored in the dark. Samples were taken at 0 days, 1 month, 2 months, 3 months and 6 months. After sampling, 1g of granules was accurately weighed, extracted with methanol by ultrasonication and diluted to volume and filtered. The filtrate was used as the test solution. Then, using the same HPLC chromatographic conditions as the active ingredient dissolution test, the reference solution and the test solution were injected to determine the peak area, and the active ingredient content was calculated by the external standard method. Finally, the retention rate was calculated based on the content at 0 days. A retention rate of ≥90% at 6 months was considered to be qualified for stability. The test was based on the Chinese Pharmacopoeia.

[0054] The obtained test data are recorded in Table 1 below:

[0055] By comparing and analyzing the relevant data in Table 1, it can be seen that the traditional Chinese medicine composition prepared by the present invention for improving depression, its preparation method, and its application not only has high dissolution rate of effective components and high blood-brain barrier permeability, but also high retention rate of effective components after accelerated testing, i.e., high component stability. This indicates that the traditional Chinese medicine composition for improving depression, its preparation method, and its application provided by the present invention have a broader market prospect and are more suitable for promotion.

[0056] In the description of this specification, references to terms such as "an embodiment," "example," and "specific example" indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the invention. In this specification, illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0057] The preferred embodiments of the present invention disclosed above are merely illustrative of the invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the invention to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of the invention, thereby enabling those skilled in the art to better understand and utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. A traditional Chinese medicine composition for improving depression, characterized in that, The ingredients include the following parts by weight: 30-50 parts of modified jujube seed extract, 25-40 parts of Albizia bark, 20-35 parts of vinegar-processed modified Bupleurum root, 15-25 parts of Angelica sinensis, 12-20 parts of white peony root, 10-18 parts of Polygala tenuifolia, 15-20 parts of Poria cocos, and 5-10 parts of functional adjuvants. The functional additive is composed of a synergist, a penetration enhancer, and a stabilizer, with the mass ratio of each raw material being (10-15):(3-5):(2-4). The preparation method of the functional additive is as follows: add the synergistic carrier to the reaction vessel, heat to 50-60℃, stir at 80-100 rpm for 20-30 min, add the penetration promoter and stabilizer and continue stirring for 20-30 min, spray dry the obtained product, pulverize it through a 180-200 mesh sieve to obtain the functional additive.

2. The traditional Chinese medicine composition for improving depression according to claim 1, characterized in that: The synergistic carrier is a modified inulin, the penetration enhancer is menthol, the stabilizer is vitamin E, and the inlet air temperature of the spray dryer is 160-180℃, and the outlet air temperature is 80-90℃.

3. The traditional Chinese medicine composition for improving depression according to claim 1, characterized in that, The specific preparation steps of the modified jujube seed extract are as follows: Jujube seed powder obtained by rinsing with deionized water and pulverizing and sieving is added to an ethanol aqueous solution, then placed in a constant temperature water bath at 50-60℃ for 1-2 hours, with stirring every 20 minutes at a speed of 100-150 rpm. After extraction, the residue is collected by filtration. The residue is added to the modification solution and stirred in a constant temperature water bath at 70-80℃ at a speed of 120-150 rpm for 1-1.5 hours. Then, centrifugation is performed to collect the precipitate of the acid-modified active ingredients. The precipitate is dried at 105-115℃ to constant weight, pulverized, and passed through a 150-200 mesh sieve. The sieve-passing material is collected to obtain the modified jujube seed extract.

4. The traditional Chinese medicine composition for improving depression according to claim 3, characterized in that: The added ethanol-water solution is 3-5 times the mass of the crude jujube seed powder, and the volume fraction of ethanol is 60-70%; the modified solution is prepared by mixing glacial acetic acid and deionized water at a mass ratio of 2:1, with a pH of 2.0-2.5, and the solid-liquid ratio of the filter residue to the modified solution is 1:(8-10).

5. The traditional Chinese medicine composition for improving depression according to claim 1, characterized in that, The method for preparing vinegar-modified Bupleurum is as follows: Bupleurum root is washed with deionized water and sliced ​​to obtain raw Bupleurum slices; the raw Bupleurum slices are added to vinegar solution, mixed well, and placed in a sealed container, and kept moist at a constant temperature of 25-30℃ for 4-6 hours, turning once every 1 hour during this period; the moistened Bupleurum slices are stir-fried at 150-160℃ for 5-8 minutes, until the surface of the Bupleurum slices turns yellowish-brown, a strong roasted aroma is emitted and there are no burnt spots, and then they are immediately removed; finally, they are pulverized and passed through a 100-120 mesh sieve to obtain the sieve-less material, thus obtaining vinegar-modified Bupleurum.

6. The traditional Chinese medicine composition for improving depression according to claim 5, characterized in that: The vinegar solution is prepared by mixing rice vinegar and deionized water at a mass ratio of 1:5 and stirring, with a pH of 3.0-3.

5. The solid-liquid ratio of Bupleurum root slices to the vinegar solution is 1:(3-4).

7. The traditional Chinese medicine composition for improving depression according to claim 2, characterized in that, The preparation steps of the inulin-modified product are as follows: Step 1: Preparation of 6-bromo-6-deoxyinulin: Inulin was dispersed in N,N-dimethylformamide, N-bromosuccinimide was added, the temperature was lowered to 0℃, and a triphenylphosphine solution dissolved in N,N-dimethylformamide was slowly added dropwise. After the addition was completed, the reaction was carried out in an ice-water bath under nitrogen protection for 30 min, and then the temperature was raised to 80℃ and the reaction was continued for 3 h. The reaction solution was poured into 5 times the volume of acetone, and the precipitate was allowed to stand for 2 h. After filtration, the precipitate was washed 3 times with acetone and lyophilized to obtain the product. Step 2, Preparation of hydrophobic 6-bromo-6-deoxyinulin: Dissolve the product from Step 1 in anhydrous pyridine, add acetic anhydride, react at room temperature for 12 h under nitrogen protection, pour the reaction solution into an ice-water bath and stir to precipitate, filter, wash with deionized water until neutral, and freeze dry to obtain the product. Step 3, Preparation of 6-azido-6-deoxyinulin: Dissolve the product from Step 2 in N,N-dimethylformamide, add sodium azide, stir and react at 80°C for 4 hours under nitrogen protection, pour the reaction solution into 3 times the volume of ice water, let it stand to precipitate for 1 hour, filter and wash the precipitate twice with ice water, freeze dry to obtain the product. Step 4, Preparation of 6-amino-6-deoxyinulin: Dissolve the product from step 3 in N,N-dimethylformamide, add triphenylphosphine, react at 60℃ for 12 h, add a 3:1 volume ratio of diethyl ether-ethanol mixed solution to precipitate, filter, wash the precipitate 3 times with anhydrous ethanol, and freeze-dry to obtain the inulin-modified product.

8. The traditional Chinese medicine composition for improving depression according to claim 7, characterized in that: The molar ratio of inulin to N-bromosuccinimide is 1:1.2, and the molar ratio of triphenylphosphine to N-bromosuccinimide is 1:1; the molar ratio of the product obtained in step one to acetic anhydride is 1:3; the molar ratio of the product obtained in step two to sodium azide is 1:1.5; and the molar ratio of the product in step three to triphenylphosphine is 1:1.

3.

9. A method for preparing a traditional Chinese medicine composition for improving depression, comprising the steps described in any one of claims 1-8: S1: Grind Angelica sinensis, Paeonia lactiflora, Polygala tenuifolia, and Poria cocos into powder, pass them through a 100-120 mesh sieve, and obtain fine powder of each herb for later use; S2: Take Albizia bark, remove impurities and cut it into small sections of 2-3cm. Add 5 times its weight of licorice and deionized water in a mass ratio of 1:

20. Boil for 30 minutes and filter to obtain a decoction. Boil at 85℃ for 1.2 hours. After filtration, take the residue, drain at room temperature and crush to 110 mesh to obtain pretreated Albizia bark. S3: Mix the modified jujube seed extract, vinegar-modified Bupleurum chinense, pretreated Albizia julibrissin bark, and the fine powder of each single herb obtained in step S1 evenly. Add 6 times the total mass of the mixed powder to deionized water, soak at 65℃ for 35 minutes, then heat to 95℃ and decoct for 1.8 hours. Filter with a 200-mesh filter cloth and collect the first filtrate. Add 4 times the mass of the residue to deionized water, decoct at 95℃ for 1.2 hours, filter and collect the second filtrate, and combine the two filtrates. S4: Place the combined filtrate in a vacuum concentration tank, control the vacuum degree to -0.085MPa and the temperature to 65℃, and concentrate under reduced pressure to a clear paste with a relative density of 1.18; S5: Add the prescribed amount of functional adjuvant to the extract, stir at 55℃ and 90rpm for 30 minutes until the mixture is uniform, then feed it into a granulator to granulate. The granules are dried at 60℃ and sieved through an 18-mesh sieve to obtain the traditional Chinese medicine composition.

10. The application of a traditional Chinese medicine composition for improving depression, wherein the composition is used in any one of claims 1-8, characterized in that: The traditional Chinese medicine composition is used in the preparation of drugs or health products to improve depressive symptoms, which include low mood, insomnia, anxiety, irritability, chest tightness, and difficulty concentrating.

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