Application of qi and blood foundation strengthening oral liquid in preparation of body building and anti-aging medicine

Abstract

The invention provides application of qi-blood foundation strengthening oral liquid in preparation of a medicine for building body and resisting aging, and belongs to the technical field of medicine preparations. According to the invention, the mass ratio relationship among the components and the preparation method thereof are researched, and especially in the preparation process, the traditional Chinese medicine components are extracted by adopting the modes of high-pressure steam heating, vacuum concentration and the like under different pressures in batches and stages, so that the obtained oral liquid for consolidating qi and blood not only has the effective component content, but also has the health-care effect. The oral liquid for consolidating qi and blood has the effects of improving the activity of superoxide dismutase of red blood cells and reducing the content of lipofuscin in tissues, can improve the exercise tolerance of rats, and has the effects of resisting aging and building the body.

Description

Technical Field

[0001] This invention belongs to the field of pharmaceutical preparation technology, specifically relating to the application of Qi Xue Gu Ben Oral Liquid in the preparation of drugs for strengthening the body and anti-aging. Background Technology

[0002] As the aging population increases, people enter middle and old age, and due to the degeneration of organs such as the thymus, their immune function weakens, enzyme activity decreases, and trace elements are deficient, making them highly susceptible to diseases of the blood vessels, bones and muscles, as well as mental and neurological diseases.

[0003] Immunity is the body's own defense mechanism, its ability to recognize and eliminate any foreign invaders (viruses, bacteria, etc.), process aging, damaged, dead, and degenerated cells, and identify and deal with mutated and virus-infected cells. To maintain good health in daily life and have abundant energy at work, it's essential to boost one's immunity to resist external threats. Therefore, anti-aging and strengthening the body are receiving increasing attention.

[0004] In response, existing technologies have conducted extensive research on pharmaceutical compositions with effects such as strengthening the body, enhancing immunity, and anti-aging. For example, Chinese patent CN111773362A discloses an anti-aging composition whose active ingredients are prepared from the following raw materials in parts by weight: 600-800 parts deer antler, 150-200 parts astragalus, 180-240 parts yam, 200-260 parts notoginseng, 200-240 parts mulberry, 200-300 parts polygonatum, 60-100 parts wolfberry, and 100-150 parts ginger. This composition effectively compensates for the shortcomings of traditional Chinese medicine formulas, which focus solely on tonifying the kidneys and boosting yang, heavily relying on kidney-tonifying herbs while neglecting the important roles of spleen-tonifying and blood-activating herbs. It has the effect of improving the aging process, delaying brain aging, improving brain function, and enhancing the body's immune function.

[0005] Chinese patent CN106727799A discloses a health-preserving traditional Chinese medicine composition, consisting of cordyceps, ginseng, and Panax notoginseng. After extensive formulation research and clinical screening trials, the composition, consisting of 20-60 parts cordyceps, 20-60 parts ginseng, and 20-60 parts Panax notoginseng, has significant effects on improving the body's immune regulation, enhancing vitality, anti-aging, regulating endocrine function, beautifying the skin and removing blemishes, calming the mind and nourishing the brain.

[0006] For example, Chinese patent CN104288245A discloses a pharmaceutical composition for anti-aging and physical enhancement, along with its preparation and detection methods. The pharmaceutical composition is made from the following ingredients: total ginsenosides, dried Ganoderma lucidum extract, Astragalus membranaceus extract, Epimedium extract, Paeonia lactiflora extract, Acanthopanax senticosus extract, and vitamin E. This pharmaceutical composition has the effects of tonifying the spleen and kidneys, nourishing yin and blood, and improving intelligence and calming the mind. It is suitable for fatigue, palpitations, insomnia, forgetfulness, and frequent urination caused by aging and physical decline.

[0007] However, existing drug compositions cannot effectively enhance the activity of superoxide dismutase in erythrocytes and reduce the content of lipofuscin in tissues, thus failing to achieve anti-aging effects at the root. Therefore, it is necessary to develop an oral liquid for strengthening the body and improving immunity, which can achieve anti-aging and improve health at the root, and its application. Summary of the Invention

[0008] Based on the shortcomings of existing technologies, this invention uses Qi Xue Gu Ben oral liquid as a foundation and studies the mass ratio between components and its preparation method. In particular, by studying its preparation method, different solvents are used to extract the Chinese herbal ingredients during the preparation process to obtain the Qi Xue Gu Ben oral liquid of this invention. This Qi Xue Gu Ben oral liquid has the effect of increasing the activity of superoxide dismutase in erythrocytes and reducing the content of lipofuscin in tissues, and can also improve the exercise endurance of rats, that is, it has the effects of anti-aging and strengthening the body.

[0009] This invention is implemented as follows: On the one hand, this invention provides the application of Qi and Blood Strengthening Oral Liquid in the preparation of drugs with the functions of strengthening the body and anti-aging.

[0010] Furthermore, the drug is one that can prolong the survival time of mice under normobaric hypoxia conditions.

[0011] Furthermore, the drug is one that can improve the exercise endurance of rats.

[0012] Furthermore, the drug is one that can improve the heat tolerance of mice and rats.

[0013] Furthermore, the drug is one that can increase superoxide dismutase in mouse erythrocytes.

[0014] Furthermore, the drug is one that can reduce the content of lipofuscin in the heart and brain tissue of mice.

[0015] Preferably, the drug is a drug that can prolong the survival time of mice under normobaric hypoxia, improve the exercise endurance of rats, improve the high temperature tolerance of mice and rats, increase the superoxide dismutase in mouse erythrocytes, and reduce the lipofuscin content in the heart and brain tissues of mice.

[0016] Preferably, the Qi and Blood Strengthening Oral Liquid is prepared by the following method: (1) Weigh lotus seeds and pine nuts, crush them, and then soak them to obtain soaking liquid for later use; (2) Weigh the rose hips and extract them to obtain extract A, which is then set aside. (3) Weigh out Acanthopanax senticosus, Schisandra chinensis, Epimedium, Morinda officinalis, Cuscuta chinensis, Ligustrum lucidum, Anemarrhena asphodeloides, Phellodendron chinense, Lycium barbarum, Rehmannia glutinosa, Polygonum multiflorum, Polygonatum sibiricum, Rubus idaeus, Dioscorea opposita, Ziziphus jujuba var. spinosa, pine needles and Platycladus orientalis seeds for extraction to obtain extract B, which is used for later use; (4) Take the soaking solution, extract A and extract B and concentrate them to obtain the extract; mix the extract with honey and purified water to obtain the Qi and Blood Strengthening Oral Liquid.

[0017] The particle size of the pulverized material in step (1) above is 250μm-850μm; the ratio of water added to the mass of the medicinal material during soaking is 4-6:1; preferably 4:1; the soaking time is 3-5 hours; preferably 4 hours.

[0018] In step (1) above, stir for 2-5 minutes every 30 minutes during the soaking process.

[0019] In step (2) above, the ratio of water added to the mass of medicinal materials is 5-8:1, preferably 6:1; the extraction is by decoction, and the number of decoctions is 2-3, preferably 2; the first decoction time is 1.5-2.5 hours, preferably 2 hours, and the second decoction time is 0.5-1.5 hours, preferably 1 hour.

[0020] The extraction process in step (3) above involves a water-to-medicinal-material ratio of 5-8:1, preferably 6:1. The extraction is performed by decoction, which is performed 2-3 times, preferably 2 times. The first decoction takes 3-6 hours, preferably 5 hours, and the second decoction takes 2-4 hours, preferably 3 hours.

[0021] The extraction described in step (3) above needs to be carried out under high-pressure steam heating conditions.

[0022] The steam pressure is 0.05-0.2 MPa from the initial heating to before boiling, 0.01-0.05 MPa within 1 hour after boiling, and 0.1-0.15 MPa from 1 hour after boiling to the end.

[0023] The temperature in step (3) above is 95-100℃.

[0024] The concentration pressure in step (4) above is 0.01-0.15 MPa, the vacuum degree is less than 0.08 MPa, the temperature is 55-75℃, and the concentration is stopped when the relative density is 1.16-1.21 (60-70℃).

[0025] The Qi and Blood Strengthening Oral Liquid, by weight, comprises the following components: 70-90 parts of Rosa rugosa fruit, 70-90 parts of Acanthopanax senticosus, 20-30 parts of Schisandra chinensis, 20-30 parts of Epimedium brevicornu, 5-10 parts of Morinda officinalis, 5-10 parts of Cuscuta chinensis, 5-10 parts of Ligustrum lucidum, 15-30 parts of Anemarrhena asphodeloides, 15-30 parts of Phellodendron chinense, 5-10 parts of Lycium barbarum, 30-50 parts of Nelumbo nucifera, 5-10 parts of Rehmannia glutinosa, 15-30 parts of Polygonum multiflorum, 10-20 parts of Polygonatum sibiricum, 20-30 parts of Rubus idaeus, 5-15 parts of Dioscorea opposita, 30-50 parts of Pine nuts, 10-15 parts of Ziziphus jujuba var. spinosa, 5-15 parts of pine needles, and 5-15 parts of Platycladus orientalis.

[0026] Preferably, the Qi and Blood Strengthening Oral Liquid comprises, by weight, the following components: 75-85 parts of Rosa rugosa fruit, 75-85 parts of Acanthopanax senticosus, 22-28 parts of Schisandra chinensis, 22-28 parts of Epimedium brevicornu, 6-8 parts of Morinda officinalis, 6-8 parts of Cuscuta chinensis, 6-8 parts of Ligustrum lucidum, 20-25 parts of Anemarrhena asphodeloides, 20-25 parts of Phellodendron chinense, 6-8 parts of Lycium barbarum, 35-45 parts of Nelumbo nucifera, 6-8 parts of Rehmannia glutinosa, 20-25 parts of Polygonum multiflorum, 12-18 parts of Polygonatum sibiricum, 22-28 parts of Rubus idaeus, 8-12 parts of Dioscorea opposita, 35-45 parts of Pine nuts, 12-14 parts of Ziziphus jujuba var. spinosa, 8-12 parts of pine needles, and 8-12 parts of Platycladus orientalis.

[0027] Preferably, the Qi and Blood Strengthening Oral Liquid comprises, by weight, the following components: 80 parts of Rosa rugosa fruit, 80 parts of Acanthopanax senticosus, 25 parts of Schisandra chinensis, 25 parts of Epimedium, 8 parts of Morinda officinalis, 8 parts of Cuscuta chinensis, 8 parts of Ligustrum lucidum, 20 parts of Anemarrhena asphodeloides, 20 parts of Phellodendron chinense, 8 parts of Lycium barbarum, 40 parts of Nelumbo nucifera, 8 parts of Rehmannia glutinosa, 25 parts of Polygonum multiflorum, 15 parts of Polygonatum sibiricum, 25 parts of Rubus idaeus, 10 parts of Dioscorea opposita, 40 parts of Pine nut, 12 parts of Ziziphus jujuba var. spinosa, 10 parts of pine needles, and 10 parts of Platycladus orientalis seed.

[0028] Compared with the prior art, the present invention has the following beneficial effects: 1. This invention studies the mass ratio between components and their preparation methods, especially the preparation methods. In the preparation process, the traditional Chinese medicine components are extracted by batch extraction, pressure extraction, and pressure concentration to obtain the Qi and Blood Strengthening Oral Liquid of this invention. This Qi and Blood Strengthening Oral Liquid can improve the activity of superoxide dismutase in erythrocytes and reduce the content of lipofuscin in tissues. It can also improve the exercise endurance of rats, that is, it has the effects of anti-aging and strengthening the body.

[0029] Especially when extracting seventeen kinds of medicinal materials such as Acanthopanax senticosus and Schisandra chinensis, high-pressure steam heating is used for extraction. The steam pressure is controlled from the initial heating to boiling at 0.05-0.2 MPa, and within 1 hour after boiling at 0.01-0.05 MPa; and from 1 hour after boiling to the end at 0.1-0.15 MPa. This helps to quickly dissolve the active ingredients, increases the content of active ingredients in the extract, and is more conducive to the efficacy of the medicine. Controlling the pressure to 0.01-0.15 MPa, the vacuum degree to less than 0.08 MPa, and the temperature to 55-75℃ during the drug concentration process can better reduce the loss of the active ingredients in the drug and retain the active ingredients in the drug to a greater extent, thereby ensuring the stability of the drug.

[0030] 2. In the formula of this invention, Acanthopanax senticosus, Rosa rugosa, Schisandra chinensis, and Ziziphus jujuba var. spinosa are the principal herbs, accompanied by Anemarrhena asphodeloides, Phellodendron chinense, Rehmannia glutinosa, and Polygonum multiflorum as assistant herbs, and Epimedium brevicornu, Morinda officinalis, Cuscuta chinensis, and Rubus idaeus as adjuvant herbs, and supplemented by Platycladus orientalis seed and pine needles as adjuvant herbs. The overall formula focuses on regulating and tonifying qi and blood, strengthening the spleen and kidneys, and calming the mind and spirit. The principal, assistant, adjuvant, and adjuvant herbs complement each other, and the medicinal properties are coordinated. It can not only improve insomnia and palpitations, but also strengthen the spleen and replenish qi, nourish yin and reduce fire, and tonify the kidneys and replenish essence. The overall formula reflects the characteristics of traditional Chinese medicine's syndrome differentiation and treatment. In the preparation process, the various extract components are comprehensively utilized and reasonably matched, which can be efficiently absorbed to exert their effects. At the same time, through specific extracts and extraction methods, the resulting oral liquid for strengthening qi and blood has a high content of effective components, and has significant effects on prolonging life, anti-aging, and strengthening the body. Detailed Implementation

[0031] The following non-limiting embodiments are intended to enable those skilled in the art to gain a more comprehensive understanding of the present invention, but do not limit the invention in any way. The following content is merely an exemplary description of the scope of protection claimed by the present invention, and those skilled in the art can make various changes and modifications to the present invention based on the disclosed content, and such changes should also fall within the scope of protection claimed by the present invention.

[0032] When numerical ranges are given in the embodiments, it should be understood that, unless otherwise stated in the invention, both endpoints of each numerical range and any value between the two endpoints may be selected. Unless otherwise defined, all technical and scientific terms used in this invention have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0033] The present invention will be further described below by way of specific embodiments. Unless otherwise specified, all chemical reagents used in the embodiments of the present invention are obtained through conventional commercial means.

[0034] In the following examples, “part” and “g” have the same meaning, that is, 1 part = 1g.

[0035] Example 1: A Qi and Blood Strengthening Oral Liquid and its Preparation Method The ingredients are as follows by weight: 80 parts of Rosa rugosa fruit, 80 parts of Acanthopanax senticosus, 25 parts of Schisandra chinensis, 25 parts of Epimedium, 8 parts of Morinda officinalis, 8 parts of Cuscuta chinensis, 8 parts of Ligustrum lucidum, 20 parts of Anemarrhena asphodeloides, 20 parts of Phellodendron chinense, 8 parts of Lycium barbarum, 40 parts of Nelumbo nucifera, 8 parts of Rehmannia glutinosa, 25 parts of Polygonum multiflorum, 15 parts of Polygonatum sibiricum, 25 parts of Rubus idaeus, 10 parts of Dioscorea opposita, 40 parts of Pine nuts, 12 parts of Ziziphus jujuba var. spinosa, 10 parts of pine needles, and 10 parts of Platycladus orientalis seed.

[0036] The preparation method is as follows: (1) Weigh lotus seeds and pine nuts and crush them to 500μm. Then add 4 times the amount of water and soak for 4 hours. Stir for 5 minutes every 30 minutes during the soaking process to obtain the soaking solution for later use. (2) Weigh out the rose hips and add 6 times the amount of water for decoction extraction. The first extraction time is 2 hours and the second decoction time is 1 hour. Combine the decoctions to obtain extract A for later use. (3) Weigh out Acanthopanax senticosus, Schisandra chinensis, Epimedium, Morinda officinalis, Cuscuta chinensis, Ligustrum lucidum, Anemarrhena asphodeloides, Phellodendron chinense, Lycium barbarum, Rehmannia glutinosa, Polygonum multiflorum, Polygonatum sibiricum, Rubus idaeus, Dioscorea opposita, Ziziphus jujuba var. spinosa, pine needles and Platycladus orientalis seeds, add 6 times the amount of water and decoct for extraction twice. The first extraction time is 5 hours and the second decoction time is 3 hours. The extraction must be carried out under high pressure steam heating. During the extraction process, the steam pressure should be controlled as follows: from the initial heating to before boiling, the steam pressure is 0.1 MPa; within 1 hour after boiling, the steam pressure is 0.03 MPa; from 1 hour after boiling to the end, the steam pressure is 0.12 MPa and the temperature is 95℃. Combine the decoction liquids to obtain extract B, which is ready for use. (4) Take the soaking solution, extract A and extract B and concentrate them. The concentration steam pressure is 0.03-0.06 MPa, the vacuum degree is -0.06 to -0.08 MPa, the temperature is 55-60℃, and the concentration is carried out until the relative density is 1.16-1.21 (60-70℃) to obtain the extract. Mix the extract with honey at a mass ratio of 1:1, and then add purified water to mix to obtain the Qi and Blood Strengthening Oral Liquid.

[0037] Example 2: A Qi and Blood Strengthening Oral Liquid and its Preparation Method The ingredients are as follows by weight: 70 parts of Rosa rugosa fruit, 90 parts of Acanthopanax senticosus, 20 parts of Schisandra chinensis, 30 parts of Epimedium, 10 parts of Morinda officinalis, 5 parts of Cuscuta chinensis, 5 parts of Ligustrum lucidum, 30 parts of Anemarrhena asphodeloides, 30 parts of Phellodendron chinense, 10 parts of Lycium barbarum, 30 parts of Nelumbo nucifera, 10 parts of Rehmannia glutinosa, 15 parts of Polygonum multiflorum, 10 parts of Polygonatum sibiricum, 30 parts of Rubus idaeus, 5 parts of Dioscorea opposita, 50 parts of Pine nuts, 10 parts of Ziziphus jujuba var. spinosa, 5 parts of pine needles, and 15 parts of Platycladus orientalis seed.

[0038] The preparation method is as follows: (1) Weigh lotus seeds and pine nuts and crush them to 500μm. Then add 6 times the amount of water and soak for 3 hours. Stir for 5 minutes every 30 minutes during the soaking process to obtain the soaking solution for later use. (2) Weigh out the rose hips and add 5 times the amount of water for decoction extraction. The first extraction time is 2.5 hours and the second decoction time is 1.5 hours. Combine the decoctions to obtain extract A for later use. (3) Weigh out Acanthopanax senticosus, Schisandra chinensis, Epimedium, Morinda officinalis, Cuscuta chinensis, Ligustrum lucidum, Anemarrhena asphodeloides, Phellodendron chinense, Lycium barbarum, Rehmannia glutinosa, Polygonum multiflorum, Polygonatum sibiricum, Rubus idaeus, Dioscorea opposita, Ziziphus jujuba var. spinosa, pine needles and Platycladus orientalis seeds, add 6 times the amount of water and decoct twice for extraction. The first extraction time is 3 hours and the second extraction time is 4 hours. The extraction should be carried out under high pressure steam heating. During the extraction process, the steam pressure should be controlled as follows: from the initial heating to before boiling, the steam pressure should be 0.05 MPa; within 1 hour after boiling, the steam pressure should be 0.05 MPa; from 1 hour after boiling to the end, the steam pressure should be 0.1 MPa and the temperature should be 98℃. Combine the decoctions to obtain extract B, which is ready for use. (4) Take the soaking solution, extract A and extract B and concentrate them. The concentration steam pressure is 0.03-0.06 MPa, the vacuum degree is -0.06 to -0.08 MPa, the temperature is 55-60℃, and the concentration is carried out until the relative density is 1.16-1.21 (60-70℃) to obtain the extract. Mix the extract with honey at a mass ratio of 1:1, and then add purified water to mix to obtain the Qi and Blood Strengthening Oral Liquid.

[0039] Example 3: A Qi and Blood Strengthening Oral Liquid and its Preparation Method The ingredients are as follows by weight: 85 parts of Rosa rugosa fruit, 75 parts of Acanthopanax senticosus, 28 parts of Schisandra chinensis, 22 parts of Epimedium, 6 parts of Morinda officinalis, 6 parts of Cuscuta chinensis, 6 parts of Ligustrum lucidum, 20 parts of Anemarrhena asphodeloides, 20 parts of Phellodendron chinense, 8 parts of Lycium barbarum, 45 parts of Nelumbo nucifera, 6 parts of Rehmannia glutinosa, 20 parts of Polygonum multiflorum, 18 parts of Polygonatum sibiricum, 22 parts of Rubus idaeus, 12 parts of Dioscorea opposita, 45 parts of Pine nuts, 12 parts of Ziziphus jujuba var. spinosa, 12 parts of pine needles, and 8 parts of Platycladus orientalis seeds.

[0040] The preparation method is as follows: (1) Weigh lotus seeds and pine nuts and crush them to 500μm. Then add 5 times the amount of water and soak for 3 hours. Stir for 5 minutes every 30 minutes during the soaking process to obtain the soaking solution for later use. (2) Weigh out the rose hips and add 8 times the amount of water for decoction extraction. The first extraction time is 1.5 hours and the second decoction time is 0.5 hours. Combine the decoctions to obtain extract A for later use. (3) Weigh out Acanthopanax senticosus, Schisandra chinensis, Epimedium, Morinda officinalis, Cuscuta chinensis, Ligustrum lucidum, Anemarrhena asphodeloides, Phellodendron chinense, Lycium barbarum, Rehmannia glutinosa, Polygonum multiflorum, Polygonatum sibiricum, Rubus idaeus, Dioscorea opposita, Ziziphus jujuba var. spinosa, pine needles and Platycladus orientalis seeds, add 6 times the amount of water and decoct twice for extraction. The first extraction time is 6 hours and the second extraction time is 2 hours. The extraction should be carried out under high pressure steam heating. During the extraction process, the steam pressure should be controlled as follows: from the initial heating to before boiling, the steam pressure should be 0.2 MPa; within 1 hour after boiling, the steam pressure should be 0.01 MPa; from 1 hour after boiling to the end, the steam pressure should be 0.1 MPa and the temperature should be 95℃. Combine the decoctions to obtain extract B, which is ready for use. (4) Take the soaking solution, extract A and extract B and concentrate them. The concentration steam pressure is 0.03-0.06 MPa, the vacuum degree is -0.06 to -0.08 MPa, the temperature is 55-60℃, and the concentration is carried out until the relative density is 1.16-1.21 (60-70℃) to obtain the extract. Mix the extract with honey at a mass ratio of 1:1, and then add purified water to mix to obtain the Qi and Blood Strengthening Oral Liquid.

[0041] Comparative Example 1 The difference from Example 1 lies in the preparation method, as detailed below: Weigh the medicinal materials used in the formula of Example 1, crush the lotus seeds and pine nuts to 500μm, soak them in 4 times the amount of water for 4 hours, stir, filter, and set aside the filtrate; boil the rose hips twice with 6 times the amount of water, the first time for 2 hours and the second time for 1 hour, filter, combine the filtrates, and set aside; boil the remaining medicinal materials twice with 6 times the amount of water, the first time for 3 hours and the second time for 2 hours, filter, combine the filtrates, combine with the above medicinal liquid, heat and concentrate to a thick paste with a relative density of not less than 1.10 (20℃), add honey and mix with the thick paste in a 1:1 ratio, then add purified water and mix, stir well, dispense and sterilize to obtain the final product.

[0042] Comparative Example 2 The difference from Example 1 is that: in step (3), the steam pressure is controlled to be 0.25 MPa from the initial heating to before boiling, and 0.08 MPa within 1 hour after boiling; the steam pressure is 0.18 MPa from 1 hour after boiling to the end, and the temperature is 95°C. The rest is the same as in Example 1.

[0043] Comparative Example 3 The difference from Example 1 is that the specific operation of step (3) is different, specifically: (3) Weigh out Acanthopanax senticosus, Schisandra chinensis, Epimedium, Morinda officinalis, Cuscuta chinensis, Ligustrum lucidum, Anemarrhena asphodeloides, Phellodendron chinense, Lycium barbarum, Rehmannia glutinosa, Polygonum multiflorum, Polygonatum sibiricum, Rubus idaeus, Dioscorea opposita, Ziziphus jujuba var. spinosa, pine needles and Platycladus orientalis seeds, add 6 times the amount of water and decoct once for 8 hours. The extraction should be carried out under high pressure steam heating. During the extraction process, the steam pressure should be controlled as follows: from the initial heating to before boiling, the steam pressure should be 0.2 MPa; within 1 hour after boiling, the steam pressure should be 0.01 MPa; from 1 hour after boiling to the end, the steam pressure should be 0.1 MPa and the temperature should be 95℃. Combine the decoctions to obtain extract B, which is ready for use.

[0044] Comparative Example 4 The difference from Example 1 is that the concentration pressure in step (4) is 0.2 MPa, the vacuum degree is -0.08 MPa, the temperature is 60°C, and the rest is the same as in Example 1.

[0045] Effect test: I. Detection of active ingredient content: (1) Berberine hydrochloride Determined by high performance liquid chromatography (General Chapter 0512, Chinese Pharmacopoeia 2025).

[0046] Chromatographic conditions and system suitability test: Octadecylsilane-bonded silica gel was used as the stationary phase, and acetonitrile-0.1% phosphoric acid solution (containing 0.1 mL of triethylamine per 100 mL) (30:70) was used as the mobile phase; the detection wavelength was 345 nm. The theoretical plate number, calculated based on the berberine hydrochloride peak, should not be less than 2000.

[0047] Preparation of reference solution: Take an appropriate amount of berberine hydrochloride reference standard, accurately weigh it, and add methanol to prepare a solution containing 6 μg per 1 mL.

[0048] Preparation of the test solution: Accurately measure 5 mL of this product and place it in a 25 mL volumetric flask. Add an appropriate amount of hydrochloric acid-methanol (1:99) mixed solution, shake, and then dilute to the mark with hydrochloric acid-methanol (1:99) mixed solution. Shake well, filter, and take the filtrate to obtain the test solution.

[0049] Assay: Accurately pipette 20 μL each of the reference solution and the test solution into the liquid chromatograph. Measure the solution to obtain the final result.

[0050] (2) Epimedoside Determined by high performance liquid chromatography (General Chapter 0512, Chinese Pharmacopoeia 2015).

[0051] Chromatographic conditions and system suitability test: Octadecylsilane-bonded silica gel was used as the stationary phase, and acetonitrile-water (25:75) was used as the mobile phase; the detection wavelength was 270 nm. The theoretical plate number, calculated based on the icariin peak, should not be less than 1500.

[0052] Preparation of reference solution: Take an appropriate amount of icariin reference standard, accurately weigh it, and add methanol to prepare a solution containing 15 μg per 1 mL.

[0053] Preparation of the test solution: Accurately measure 10 mL of this product and place it in a 25 mL volumetric flask. Add an appropriate amount of methanol, shake, then dilute with methanol to the mark, shake well, filter, and take the filtrate to obtain the test solution.

[0054] Determination method: Accurately pipette 20 μL of the reference solution and the test solution into the liquid chromatograph and determine the result.

[0055] The test substances for the above-mentioned test samples are extract B and Qi Xue Gu Ben oral liquid obtained in Examples 1-3 and Comparative Examples 1-4, respectively.

[0056] The extraction rate is calculated as follows: W% = [(C × V) / m] × 100% in: W represents the extraction rate; C represents the concentration of berberine hydrochloride or icariin; V represents the volume of extract B or Qi and Blood Strengthening Oral Solution; m represents the mass of Phellodendron bark or Epimedium.

[0057] The test results are shown in Table 1 below.

[0058] Table 1

[0059] According to the test results in Table 1 above, the extraction efficiency of the effective components berberine hydrochloride or icariin in the Qi Xue Gu Ben oral liquid provided in Examples 1-3 of this invention is relatively high, that is, the content of effective components is relatively high, and the extraction rate can still be maintained after concentration. This indicates that the Qi Xue Gu Ben oral liquid in Examples 1-3 has a high and stable content of effective components.

[0060] In Comparative Example 1, the extraction efficiency of berberine hydrochloride or icariin obtained by conventional extraction methods was significantly reduced, and the stability was also significantly reduced.

[0061] In Comparative Example 2, changing the pressure before and after boiling during extraction significantly reduced the content of berberine hydrochloride or icariin in the product, thus decreasing the content of active ingredients.

[0062] In Comparative Example 3, changing the extraction time and the pressure before and after boiling significantly reduced the content of berberine hydrochloride or icariin in the product. Extending the extraction time actually reduced the content of active ingredients in the product, which may be due to the loss of active ingredients caused by prolonged extraction.

[0063] In Comparative Example 4, changing the concentration pressure parameters can cause a certain degree of loss of active ingredients, resulting in a decrease in the content of active ingredients. The rate of change of active ingredients is significantly higher than that in Examples 1-3.

[0064] II. Drug Efficacy Testing 1. Effect on survival time of mice under normobaric hypoxia Test substance: The oral liquid prepared in Example 1 (each vial contains berberine hydrochloride (C)). 20 H 17 0.17 mg of icariin (CNO4·HCl) was added. 33 H 40 O 15 (0.12mg total).

[0065] Mice: Kunming mice provided by the Experimental Animal Center of Tongji Medical University, male, weighing 20-22g; Methods and Results: Thirty mice were randomly divided into an experimental group and a control group, with 15 mice in each group. The control group received tap water, while the experimental group received the test substance via gavage once a day at a dose of 4 ml / kg body weight for 7 consecutive days. On the morning of the 7th day, food and water were withheld for one hour before gavage. One hour after gavage, each mouse was placed in a 250 ml specimen container containing 5 g of soda lime, sealed with Vaseline, and the survival time of each animal was recorded. Blood was collected after death by decapitation to measure lactic acid (using the micro-method and UV spectrophotometer). The results are shown in Table 2.

[0066] Table 2

[0067] According to the test results in Table 2 above, the postoperative blood lactate levels of the two groups of mice were similar, indicating that the cause of death was hypoxia, and the blood lactate threshold for death was the same. Survival time was significantly longer in the experimental group than in the control group, with a highly significant difference between the two groups (P < 0.01). The average survival time in the experimental group was 10.7 minutes longer than that in the control group, and the shortest and longest death times in the experimental group were also 6.4 minutes and 16.2 minutes longer than those in the control group, respectively. This indicates that the oral solution prepared in Example 1 can significantly prolong the survival time of mice under normobaric hypoxia.

[0068] 2. Effects on exercise endurance in rats Test substance: The oral liquid prepared in Example 1 (each vial contains berberine hydrochloride (C)). 20 H 17 0.17 mg of icariin (CNO4·HCl) was added. 33 H 40 O 15 (0.12mg total).

[0069] White rats: Male Wister rats, weighing 150-205g, provided by the Department of Nutrition and Food Hygiene, Tongji Medical University.

[0070] Methods and Results: Eighteen white rats were selected and randomly divided into an experimental group and a control group, with nine rats in each group. The control group received tap water, while the experimental group received the test substance by gavage once a day at a dose of 4 mg / kg body weight for eleven consecutive days. On the morning of the eleventh day, one hour after gavage, the rats were placed in a pool with a depth of 70 cm and a water temperature of 30°C to swim until they died. The swimming time of each rat was recorded, and the results are shown in Table 3 below.

[0071] Table 3

[0072] According to the test results in Table 3 above, there is a significant difference in swimming time between the control group and the experimental group. The swimming time of the experimental group is significantly longer than that of the control group, indicating that the oral liquid prepared in Example 1 can increase the endurance of rats.

[0073] 3. Effects on heat tolerance in mice and rats Test substance: The oral liquid prepared in Example 1 (each vial contains berberine hydrochloride (C)). 20 H 17 0.17 mg of icariin (CNO4·HCl) was added. 33 H 40 O 15 (0.12mg total).

[0074] Wister male rat; White mice: Kunming strain white mice.

[0075] 3.1 Effects on blood lactate and rectal temperature in rats under high temperature Twenty male Wistar rats, weighing 200-250g, were randomly divided into a control group and an experimental group, with 10 rats in each group. The experiment lasted for 10 days. On the first day, no test substance was given, and both groups of animals were placed in a high-temperature room at 39±1℃ for 2 hours. Tail blood was collected at the beginning (before high temperature) and the end (after high temperature) to measure lactic acid content. From the second to the tenth day, the control group was given tap water, while the experimental group was given the oral solution prepared in Example 1 by gavage at a dose of 4mL / kg body weight. The animals were also placed in the high-temperature room once a day, with food and water withheld 30 minutes to 1 hour after gavage each time. On the tenth day, tail blood was collected again before and after high temperature to measure lactic acid (micro-method), and rectal temperature was measured using a digital thermometer. The results are shown in Tables 4 and 5.

[0076] Table 4. Effects of high temperature on blood lactate levels in rats (mg / dl)

[0077] Table 5. Effects of high temperature on rat rectal temperature (°C)

[0078] Note: The temperature in the animal room is 25±3℃.

[0079] As shown in Tables 4 and 5 above, before the experiment, there was no difference in blood lactate levels between the two groups of rats before and after the high-temperature treatment. After the experiment, there was no difference in blood lactate levels before the high-temperature treatment, but after the high-temperature treatment, blood lactate levels increased in the control group and decreased in the experimental group, showing a significant difference in blood lactate levels between the two groups (0.05 > P > 0.01). The changes in rectal temperature before and after the high-temperature treatment also showed significant differences between the two groups of rats after the experiment (P < 0.001).

[0080] 3.2 Effects of high temperature on the survival time of mice Twenty male Kunming mice, weighing 20-22g, were randomly divided into an experimental group and a control group, with 10 mice in each group. Each group was administered tap water and the oral solution prepared in Example 1 via gavage once daily for seven consecutive days, at a dose of 4ml / kg body weight, respectively. On the last day, one hour after gavage, the mice were withheld from food and water and placed in a biochemical incubator at 46±2℃ until all mice died. The survival time of each mouse was recorded, and blood was collected after decapitation to measure lactic acid levels. The results are shown in Table 6.

[0081] Table 6. Effects of high temperature on mouse survival time and postmortem blood lactate levels in mice (blood collected after decapitation).

[0082] According to the test results in Table 6 above, there was no difference in blood lactate content between the two groups of animals after death, which means that the lactate limit was the same at the time of death. However, the survival time of the two groups of animals at the same high temperature was significantly different, with the experimental group surviving for an average of 16.5 minutes longer than the control group.

[0083] This indicates that the Qi and Blood Strengthening Oral Liquid prepared in Example 1 of the present invention has a good effect on improving the body temperature regulation, blood circulation and oxygen supply capacity of animals under high temperature, as well as prolonging their survival time.

[0084] 4. Effects on superoxide dismutase and lipofuscin in mouse erythrocytes Test substance: The oral liquid prepared in Example 1 (each vial contains berberine hydrochloride (C)). 20 H 17 0.17 mg of icariin (CNO4·HCl) was added. 33 H 40 O 15 (0.12mg total).

[0085] Animals: NIH mice; Animal grouping and administration: Animals were randomly divided into two groups, half male and half female, based on body weight. The weight range was 22-26g. They were fed standard pelleted feed, drank tap water, and were kept under the same temperature and humidity conditions. Animals were administered 6ml / kg via gavage, while the control group received 6ml / kg of cooled boiled water. Gavage was performed daily between 8-9 am for 16 consecutive days.

[0086] Superoxide dismutase (SOD) assay: One day before the animal was sacrificed, 50 μl of blood was collected from the tail, washed with physiological saline, and red blood cells were separated. The SOD activity of red blood cells was measured by micro-method and expressed as μg / gHb.

[0087] Lipofuscin determination: On day seventeen of the experiment, animals were euthanized by decapitation. The whole brain and heart were removed, weighed, and homogenized with chloroform:methanol (2:1). After warm dissolution and centrifugation, the lipofuscin content was determined. The sensitivity was adjusted with μg / ml quinine sulfate. The lipofuscin content was calculated as micrograms of fluorescent substance per gram of tissue, equivalent to the fluorescence intensity of the standard solution.

[0088] The test results are shown in Tables 7 and 8 below.

[0089] Table 7. Changes in SOD activity in mouse erythrocytes (μg / gHb)

[0090] According to the test results in Table 7 above, after 16 days of drug administration, the change in superoxide dismutase in mouse erythrocytes was greater in the experimental group than in the control group. Although there was no statistically significant difference, there was a clear increasing trend.

[0091] Table 8 Effects on lipofuscin content (μg / g, X±S) in the heart and brain of mice

[0092] According to the test results in Table 8 above, the oral liquid prepared in Example 1 can significantly reduce the content of lipofuscin in the heart and brain, with the most significant reduction in the content of lipofuscin in brain tissue.

[0093] The above embodiments are only used to illustrate the technical solutions of the present invention and not to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art can still make modifications or equivalent substitutions to the specific implementation schemes of the present invention, and these modifications or equivalent substitutions do not depart from the spirit and scope of the present invention, and are all within the protection scope of the claims of the present invention.

Claims

1. Application of Qi and Blood Strengthening Oral Liquid in the preparation of drugs with the functions of strengthening the body and anti-aging.

2. The application according to claim 1, characterized in that: The drug mentioned is one that can increase superoxide dismutase.

3. The application according to claim 1, characterized in that: The drug mentioned is one that can reduce the content of lipofuscin.

4. The application according to claim 1, characterized in that: The drug described is one that can increase the content of superoxide dismutase and decrease the content of lipofuscin.

5. The application according to any one of claims 1-4, characterized in that: The Qi and Blood Strengthening Oral Liquid is prepared by the following method: (1) Weigh lotus seeds and pine nuts, crush them, and then soak them to obtain soaking liquid for later use; (2) Weigh the rose hips and extract them to obtain extract A, which is then set aside. (3) Weigh out Acanthopanax senticosus, Schisandra chinensis, Epimedium, Morinda officinalis, Cuscuta chinensis, Ligustrum lucidum, Anemarrhena asphodeloides, Phellodendron chinense, Lycium barbarum, Rehmannia glutinosa, Polygonum multiflorum, Polygonatum sibiricum, Rubus idaeus, Dioscorea opposita, Ziziphus jujuba var. spinosa, pine needles and Platycladus orientalis seeds for extraction to obtain extract B, which is used for later use; (4) Take the soaking solution, extract A and extract B and concentrate them to obtain the extract; mix the extract with honey and purified water to obtain the Qi and Blood Strengthening Oral Liquid. The extraction described in step (3) needs to be carried out under high-pressure steam heating conditions; the steam pressure from the initial heating to boiling is 0.05-0.2MPa, the steam pressure is 0.01-0.05MPa within 1 hour after boiling, and the steam pressure is 0.1-0.15MPa from 1 hour after boiling to the end, and the temperature is 95-100℃.

6. The application according to claim 5, characterized in that: The particle size of the pulverized material in step (1) is 250μm-850μm; the ratio of water added to the mass of the medicinal material during soaking is 4-6:1; the soaking time is 3-5 hours; and the material is stirred for 2-5 minutes every 30 minutes during the soaking process.

7. The application according to claim 5, characterized in that: In step (2), the ratio of water added to the mass of medicinal materials is 5-8:

1. The extraction is carried out by decoction, which is carried out twice. The first decoction takes 1.5-2.5 hours, and the second decoction takes 0.5-1.5 hours.

8. The application according to claim 5, characterized in that: The extraction process in step (3) involves a water-to-medicinal-material ratio of 5-8:

1. The extraction is performed by decoction, which is repeated twice. The first decoction takes 3-6 hours, and the second decoction takes 2-4 hours.

9. The application according to claim 5, characterized in that: The Qi and Blood Strengthening Oral Liquid, by weight, comprises the following components: 70-90 parts of Rosa rugosa fruit, 70-90 parts of Acanthopanax senticosus, 20-30 parts of Schisandra chinensis, 20-30 parts of Epimedium brevicornu, 5-10 parts of Morinda officinalis, 5-10 parts of Cuscuta chinensis, 5-10 parts of Ligustrum lucidum, 15-30 parts of Anemarrhena asphodeloides, 15-30 parts of Phellodendron chinense, 5-10 parts of Lycium barbarum, 30-50 parts of Nelumbo nucifera, 5-10 parts of Rehmannia glutinosa, 15-30 parts of Polygonum multiflorum, 10-20 parts of Polygonatum sibiricum, 20-30 parts of Rubus idaeus, 5-15 parts of Dioscorea opposita, 30-50 parts of Pine nuts, 10-15 parts of Ziziphus jujuba var. spinosa, 5-15 parts of pine needles, and 5-15 parts of Platycladus orientalis.

10. The application according to claim 9, characterized in that: The Qi and Blood Strengthening Oral Liquid, by weight, comprises the following components: 80 parts of Rosa rugosa fruit, 80 parts of Acanthopanax senticosus, 25 parts of Schisandra chinensis, 25 parts of Epimedium, 8 parts of Morinda officinalis, 8 parts of Cuscuta chinensis, 8 parts of Ligustrum lucidum, 20 parts of Anemarrhena asphodeloides, 20 parts of Phellodendron chinense, 8 parts of Lycium barbarum, 40 parts of Nelumbo nucifera, 8 parts of Rehmannia glutinosa, 25 parts of Polygonum multiflorum, 15 parts of Polygonatum sibiricum, 25 parts of Rubus idaeus, 10 parts of Dioscorea opposita, 40 parts of Pine nut, 12 parts of Ziziphus jujuba var. spinosa, 10 parts of pine needles, and 10 parts of Platycladus orientalis seed.

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