Cell apoptosis inducer magnosell water-soluble protein mixing device

Abstract

The application discloses an apoptosis inducer large white tricholoma lobayense water-soluble protein mixing device, relates to the field of water-soluble protein mixing devices, and comprises a tank body, a top cover detachably mounted on the top of the tank body, a feeding pipe arranged at the top of the side of the tank body, a discharging pipe arranged at the bottom of the side of the tank body, a rotating shaft rotatably connected to the bottom of the top cover and located in the tank body, a plurality of stirring paddles circularly and equidistantly arranged at the bottom of the side of the rotating shaft, and a support frame arranged at the bottom of the inner wall of the tank body. When the rotating shaft drives the stirring paddles to rotate, the stirring paddles push the liquid around the stirring paddles to move downwards. The apoptosis inducer large white tricholoma lobayense water-soluble protein mixing device can overcome the influence of the reduced rotating speed of the stirring paddles and ensure the efficient water-soluble protein mixing work by optimizing the mixing mode of the water-soluble protein.

Description

Technical Field

[0001] This invention relates to water-soluble protein mixing equipment technology, specifically to a water-soluble protein mixing equipment for the cell apoptosis inducer *Pleurotus ostreatus*. Background Technology

[0002] Leucopaxillus giganteus, a valuable edible and medicinal fungus, is widely distributed in northern my country, including Inner Mongolia and Hebei. It is not only delicious but also rich in nutritional and medicinal value. Studies have shown that Leucopaxillus giganteus contains various bioactive substances, such as polysaccharides and caliciferine, possessing multiple biological functions including scavenging excess free radicals, enhancing immune function, and anti-tumor activity. It also has certain effects in treating measles in children and relieving irritability.

[0003] Among numerous active ingredients, the water-soluble protein from *Pleurotus ostreatus* has become a research hotspot in the biomedical field due to its unique biological characteristics. Water-soluble proteins are more easily absorbed and utilized by the human body, exerting their effects at the cellular level. Research has found that *Pleurotus ostreatus* water-soluble proteins possess the ability to induce apoptosis, a characteristic that demonstrates great potential in cancer treatment. Apoptosis is a autonomous and orderly process of cell death, crucial for maintaining bodily balance and preventing disease. During the development of cancer, reduced apoptosis in cancer cells leads to abnormal proliferation. *Pleurotus ostreatus* water-soluble proteins can promote cancer cell apoptosis by activating related apoptosis signaling pathways, providing new insights and potential drug sources for cancer treatment. For example, certain water-soluble protein components isolated from *Pleurotus ostreatus* can specifically act on cancer cells, activating the caspase family, triggering a cascade reaction, and promoting apoptosis in cancer cells, while having minimal impact on normal cells. This lays the foundation for developing highly effective and low-toxicity anticancer drugs. In addition, for some autoimmune diseases and neurodegenerative diseases caused by cell apoptosis imbalance, the water-soluble protein of *Pleurotus ostreatus* may also play a certain therapeutic and interventional role by regulating the cell apoptosis process, which has important research value and clinical application prospects.

[0004] In the process of preparing apoptosis-inducing agents from *Pleurotus ostreatus* water-soluble protein, it needs to be mixed with other components to achieve the best induction effect. However, because *Pleurotus ostreatus* water-soluble protein is sensitive to environmental factors, changes in temperature, pH, and mechanical shear force can alter its protein structure. Therefore, during the mixing process, sensors are typically used to monitor the temperature and pH of the mixture in real time for adjustment. However, reducing mechanical shear force can only be achieved by decreasing the rotation speed of the agitator blades. Traditional mixing equipment, such as propeller and turbine blades, typically accelerates material mixing by increasing their rotation speed. Therefore, reducing the agitator blade speed significantly reduces the mixing efficiency of *Pleurotus ostreatus* water-soluble protein, making the mixing process more time-consuming and thus significantly increasing the processing cost of *Pleurotus ostreatus* water-soluble protein. Summary of the Invention

[0005] The purpose of this invention is to provide a mixing device for the apoptosis inducer *Pleurotus ostreatus* water-soluble protein to overcome the above-mentioned shortcomings in the prior art.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a mixing device for apoptosis inducer *Pleurotus ostreatus* water-soluble protein, comprising a tank, a top cover detachably installed on the top of the tank, an inlet pipe provided on the top of the side of the tank, an outlet pipe provided on the bottom of the side of the tank, a rotating shaft located inside the tank being rotatably connected to the bottom of the top cover, and a plurality of stirring blades equidistantly installed in a circular shape on the bottom of the side of the rotating shaft, the stirring blades being inclined, and when the rotating shaft drives the stirring blades to rotate, the stirring blades push the surrounding liquid downwards;

[0007] A support frame is installed at the bottom of the inner wall of the tank, and a partition cylinder is installed at the top of the support frame. Several through holes are opened on the side and top surface of the partition cylinder. Four sets of flow collecting hoods are arranged in a circular shape at equal intervals on the inner wall of the partition cylinder. The side wall of the flow collecting hood and the wall surface of the partition cylinder form a fan shape, and a water outlet groove is provided at the end of all the flow collecting hoods facing the center.

[0008] A first flow-blocking plate is provided on one side of the inner wall of the flow collector near the water outlet tank, and a second flow-blocking plate is provided on the other side of the inner wall of the flow collector away from the first flow-blocking plate. The angle between the first flow-blocking plate and the inner wall of the flow collector is 120 degrees, and the angle between the second flow-blocking plate and the inner wall of the flow collector is 120 degrees.

[0009] Furthermore, the stirring blade is disposed at the bottom inside the separator cylinder, and the size of the stirring blade is adapted to the size of the inner wall of the separator cylinder.

[0010] Furthermore, an installation frame is mounted on the outside of the rotating shaft above the partition cylinder. Several vertical plates are installed at equal intervals in a circular shape at the bottom of the installation frame, and the vertical plates are slidably connected to the inner wall of the tank.

[0011] Furthermore, the support frame includes a support ring and several support rods mounted on its upper surface. The bottom surface of the inner wall of the tank is provided with an annular groove that matches the support ring. The top of the separator cylinder is provided with an opening. The top of the inner wall of the separator cylinder is provided with a support ring. Two symmetrical semicircular plates are placed on the support ring. The surface of the rotating shaft is rotatably connected to a support wheel. The side of the support wheel is provided with an annular groove. The center of each of the two semicircular plates is provided with an adapter part that matches the annular groove.

[0012] Furthermore, the diameter of the semicircular plate is matched with the diameter of the inner wall of the separator cylinder.

[0013] Furthermore, a support block is fixedly mounted on the surface of the rotating shaft, and a threaded groove extending into the interior of the support block is opened on the surface of the mounting bracket, with a locking bolt installed inside the threaded groove.

[0014] Furthermore, the mating surface of one side of the semicircular plate is provided with a plurality of slots at equal intervals along its length, and the mating surface of the other side of the semicircular plate is provided with a plurality of blocks that are adapted to the slots.

[0015] Compared with the prior art, the apoptosis inducer, *Pleurotus ostreatus* water-soluble protein mixing device provided by the present invention has the following beneficial effects:

[0016] 1. The cell apoptosis inducer, Mushroom oyster shell water-soluble protein mixing equipment, optimizes the mixing method of water-soluble protein, allowing it to undergo multiple convergence and mixing processes of varying degrees. This overcomes the impact of reduced stirring blade speed and ensures efficient mixing of water-soluble protein.

[0017] 2. This apoptosis inducer, *Pleurotus ostreatus* water-soluble protein mixing equipment, through the interplay of annular grooves, support rings, openings, support rings, semi-circular plates, and support wheels, allows the top of the separator cylinder to be removed and taken out from inside the tank. Furthermore, through the interplay of support blocks, threaded grooves, and locking bolts, the mounting frame can be disassembled as needed, allowing for thorough disassembly of the internal structure of the tank during subsequent cleaning, thus facilitating thorough cleaning of the tank's interior. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this invention. For those skilled in the art, other drawings can be obtained based on these drawings.

[0019] Figure 1 This is a schematic diagram of the overall structure provided for an embodiment of the present invention;

[0020] Figure 2 This is a schematic diagram of the internal structure of the tank provided in an embodiment of the present invention;

[0021] Figure 3 This is a schematic diagram of the transverse cross-sectional structure of the partition cylinder provided in an embodiment of the present invention;

[0022] Figure 4 This is a diagram showing the internal liquid flow state of the separator provided in an embodiment of the present invention.

[0023] Figure 5 This is a schematic diagram of the structure of the separator cylinder and the tank body in a separated state according to an embodiment of the present invention;

[0024] Figure 6 This is a schematic diagram of the structure of the semi-circular plate and the separator cylinder in a separated state according to an embodiment of the present invention;

[0025] Figure 7 This is a schematic diagram of the semi-circular plate in a separated state provided in an embodiment of the present invention;

[0026] Figure 8 Provided for embodiments of the present invention Figure 7 Enlarged structural diagram at point A;

[0027] Figure 9 This is a schematic diagram of the installation frame and support block separated in an embodiment of the present invention.

[0028] Explanation of reference numerals in the attached figures:

[0029] 1. Tank body; 101. Top cover; 102. Feed pipe; 103. Discharge pipe; 2. Rotating shaft; 21. Agitator blade; 3. Support frame; 31. Divider cylinder; 32. Through hole; 33. Flow collector; 34. Water outlet trough; 35. First baffle plate; 36. Second baffle plate; 4. Mounting frame; 41. Vertical plate; 5. Support ring; 51. Support rod; 52. Annular groove; 53. Opening; 54. Support ring; 55. Semicircular plate; 56. Support wheel; 57. Annular groove; 58. Adaptor part; 59. Support block; 510. Threaded groove; 511. Locking bolt; 6. Groove; 61. Locking block. Detailed Implementation

[0030] To enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings.

[0031] Example 1:

[0032] Please see Figures 1-5 A mixing device for apoptosis inducer *Pleurotus ostreatus* water-soluble protein includes a tank 1. The top of the tank 1 is detachably fitted with a top cover 101. The top of the side of the tank 1 is provided with a feed pipe 102, and the bottom of the side of the tank 1 is provided with a discharge pipe 103. The bottom of the top cover 101 is rotatably connected to a rotating shaft 2 located inside the tank 1. The rotating shaft 2 is driven by a motor, and the rotating shaft 2 is connected to the output shaft of the motor in a conventional way to make it easy to disassemble. Several stirring blades 21 are installed in a circular shape at equal intervals on the bottom of the side of the rotating shaft 2. The stirring blades 21 are inclined. When the rotating shaft 2 drives the stirring blades 21 to rotate, the stirring blades 21 push the surrounding liquid downward.

[0033] A support frame 3 is installed at the bottom of the inner wall of the tank 1, and a partition cylinder 31 is installed at the top of the support frame 3. Several through holes 32 are opened on the side and top surface of the partition cylinder 31. Four sets of flow collecting hoods 33 are arranged in a circular shape at equal intervals on the inner wall of the partition cylinder 31. The side wall of the flow collecting hood 33 and the wall of the partition cylinder 31 form a fan shape, and a water outlet groove 34 is provided at the end of all flow collecting hoods 33 facing the center.

[0034] It should be added that the separator 31 located between two adjacent flow collectors 33 has a water outlet trough 34.

[0035] It should be further noted that a first flow-blocking plate 35 is provided on one side of the inner wall of the flow-collecting hood 33 near the water outlet trough 34, and a second flow-blocking plate 36 is provided on the other side of the inner wall of the flow-collecting hood 33 away from the first flow-blocking plate 35.

[0036] Furthermore, the angle between the first baffle plate 35 and the inner wall of the flow collector 33 is 120 degrees, and the angle between the second baffle plate 36 and the inner wall of the flow collector 33 is 120 degrees. This allows the liquids on both sides to continue flowing after contacting the first baffle plate 35 and the second baffle plate 36 respectively. Under the guidance of the first baffle plate 35 and the second baffle plate 36, when the two liquids converge towards the middle, they can meet the liquid flowing in the middle at a smaller angle, rather than directly converging in the direction of the middle liquid's flow. This avoids significant interference with the flow of the middle liquid, while also allowing for some collision with the middle liquid, resulting in better mixing of the three liquids.

[0037] It should be further noted that the stirring blade 21 is located at the bottom inside the separator 31, and the size of the stirring blade 21 is compatible with the size of the inner wall of the separator 31. This allows the stirring blade 21 to effectively pull the liquid collected inside the separator 31 downwards when it rotates, so that it can be discharged from the bottom of the separator 31.

[0038] During the mixing process, the rotating shaft 2 drives the stirring blade 21 to rotate. The rotation of the stirring blade 21 can pull the liquid inside the separator 31 out from its bottom. After being discharged, the liquid re-enters the interior of the separator 31 through the through hole 32 provided on the separator 31.

[0039] During the above process, the liquid entering the flow collector 33 exists in multiple streams (such as...). Figure 4 As shown), the water flows include water flows a and b near the inner walls of both sides of the flow collector 33, and water flow c flowing in the middle. After entering, water flows a and b collide with the second baffle plate 36 and the first baffle plate 35 respectively, and then converge and mix at the second baffle plate 36 and the first baffle plate 35 to form water flows A and B. The mixed water flows A and B, guided by the second baffle plate 36 and the first baffle plate 35, converge and mix with the water flow c in the middle to form water flow C, which is finally discharged from the outlet trough 34 to the outside of the flow collector 33, resulting in multiple water flows C and multiple water flows D. The liquid can converge and mix inside the separator 31 from different directions. Some of the liquid enters from the inside of the separator 31 and converges and mixes with the multiple streams of water inside the separator 31. Under the traction of the stirring blade 21, it flows downward. The mixed liquid is discharged from the bottom of the separator 31 to the outside again and the above-mentioned convergence process is repeated. Thus, even when the speed of the stirring blade 21 is reduced, the same effect of efficient mixing of water-soluble protein and various materials can be achieved through multiple convergence and mixing of different degrees, which significantly improves the processing efficiency of water-soluble protein.

[0040] Example 2:

[0041] Please see Figure 5 This embodiment provides a technical solution based on the above embodiments: an installation frame 4 is installed on the outside of the rotating shaft 2 above the partition cylinder 31, and several vertical plates 41 are installed at equal intervals in a circular shape at the bottom of the installation frame 4, and the vertical plates 41 are slidably connected to the inner wall of the tank body 1.

[0042] It should be noted that the vertical plate 41 is made of hard rubber material. This material is one option, but other materials that are less likely to damage the inner wall of the tank 1 can also be used, thereby effectively preventing the accumulation of materials on the inner wall of the tank 1.

[0043] During the mixing process, the vertical plate 41 is designed to rotate synchronously with the rotating shaft 2, and pushes the surrounding liquid to move horizontally, thereby further enhancing the mixing effect of the upstream liquid.

[0044] Example 3:

[0045] Please see Figures 5-9 This embodiment provides a technical solution based on the above embodiments: the support frame 3 includes a support ring 5 and a plurality of support rods 51 installed on its upper surface. The bottom surface of the inner wall of the tank 1 is provided with an annular groove 52 that is adapted to the support ring 5. The top of the partition cylinder 31 is provided with an opening 53. The top of the inner wall of the partition cylinder 31 is provided with a support ring 54. Two symmetrical semicircular plates 55 are placed on the support ring 54. The surface of the rotating shaft 2 is rotatably connected to a support wheel 56. The side of the support wheel 56 is provided with an annular groove 57. The center of each of the two semicircular plates 55 is provided with an adapter part 58 that is adapted to the annular groove 57.

[0046] It should be noted that the diameter of the semicircular plate 55 is matched with the diameter of the inner wall of the partition cylinder 31, so that the two sets of semicircular plates 55 can fully cover the opening 53 at the top of the partition cylinder 31 after being spliced ​​together.

[0047] In this embodiment, a support block 59 is fixedly installed on the surface of the rotating shaft 2, and a threaded groove 510 extending into the support block 59 is opened on the surface of the mounting bracket 4. A locking bolt 511 is installed inside the threaded groove 510.

[0048] When the inside of the tank 1 needs to be cleaned later, the operator removes the top cover 101. When the top cover 101 is removed, the rotating shaft 2 moves synchronously. The movement of the rotating shaft 2 causes the two sets of semicircular plates 55 to separate from the separator cylinder 31. At this time, the semicircular plates 55 no longer restrict the movement of the separator cylinder 31. At this time, the separator cylinder 31 can be removed from the tank 1 by means of instruments. When the rotating shaft 2 moves, it will also cause the mounting bracket 4 to detach from the tank 1, so that no other instruments inside the tank 1 will interfere with the cleaning of its inner wall. At the same time, the removed mounting bracket 4 can be separated from the rotating shaft 2 by rotating the locking bolt 511. Furthermore, the two sets of semicircular plates 55 can be separated by applying a force to separate them, so that the inner wall of the tank 1 and the various instruments installed inside it can be thoroughly cleaned.

[0049] Example 4:

[0050] Please see Figure 8 This embodiment provides a technical solution based on the above embodiment: a plurality of slots 6 are provided at equal intervals along the length direction of the mating surface of one side semicircular plate 55, and a plurality of locking blocks 61 adapted to the slots 6 are provided on the mating surface of the other side semicircular plate 55.

[0051] This ensures that during the assembly process, when the two semicircular plates 55 are engaged in the annular grooves 57 on the support wheel 56, they can maintain a stable engagement, thus facilitating their entry into the tank 1 and splicing with the partition cylinder 31.

[0052] The foregoing has only described certain exemplary embodiments of the present invention by way of illustration. Undoubtedly, those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the foregoing drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A mixing device for apoptosis-inducing agent, *Pleurotus ostreatus* water-soluble protein, comprising a tank (1), a top cover (101) detachably mounted on the top of the tank (1), an inlet pipe (102) provided on the top of the side of the tank (1), and an outlet pipe (103) provided on the bottom of the side of the tank (1), characterized in that, The bottom of the top cover (101) is rotatably connected to a rotating shaft (2) located inside the tank (1). Several stirring blades (21) are installed in a circular shape at equal intervals on the bottom side of the rotating shaft (2). The stirring blades (21) are inclined. When the rotating shaft (2) drives the stirring blades (21) to rotate, the stirring blades (21) push the surrounding liquid downward. A support frame (3) is installed at the bottom of the inner wall of the tank (1), and a partition cylinder (31) is installed at the top of the support frame (3). Several through holes (32) are opened on the side and top surface of the partition cylinder (31). Four sets of flow collecting hoods (33) are arranged in a circular shape at equal intervals on the inner wall of the partition cylinder (31). The side wall of the flow collecting hood (33) and the wall of the partition cylinder (31) form a fan shape, and all the flow collecting hoods (33) have a water outlet groove (34) at one end facing the center. A first flow-blocking plate (35) is provided on one side of the inner wall of the flow collector (33) near the water outlet tank (34), and a second flow-blocking plate (36) is provided on the other side of the inner wall of the flow collector (33) away from the first flow-blocking plate (35). The angle between the first flow-blocking plate (35) and the inner wall of the flow collector (33) is 120 degrees, and the angle between the second flow-blocking plate (36) and the inner wall of the flow collector (33) is 120 degrees.

2. The mixed device of the water-soluble protein of the Great White Mushroom of claim 1, wherein, The stirring blade (21) is located at the bottom inside the partition cylinder (31), and the size of the stirring blade (21) is adapted to the size of the inner wall of the partition cylinder (31).

3. The mixed device of the water-soluble protein of the Great White Mushroom of Claim 2, characterized in that, The rotating shaft (2) is equipped with a mounting frame (4) located above the partition cylinder (31). The bottom of the mounting frame (4) is equipped with several vertical plates (41) at equal intervals in a circular shape. The vertical plates (41) are slidably connected to the inner wall of the tank (1).

4. The mixed device of the water-soluble protein of the Great White Mushroom of Claim 3, wherein, The support frame (3) includes a support ring (5) and several support rods (51) mounted on its upper surface. The bottom surface of the inner wall of the tank (1) is provided with an annular groove (52) that matches the support ring (5). The top of the partition cylinder (31) is provided with an opening (53). The top of the inner wall of the partition cylinder (31) is provided with a support ring (54). Two symmetrical semicircular plates (55) are placed on the support ring (54). The surface of the rotating shaft (2) is rotatably connected to a support wheel (56). The side of the support wheel (56) is provided with an annular groove (57). The center of each of the two semicircular plates (55) is provided with an adapter part (58) that matches the annular groove (57).

5. The mixed device of the water-soluble protein of the Great White Mushroom of Claim 4, wherein, The diameter of the semicircular plate (55) is matched with the diameter of the inner wall of the separator cylinder (31).

6. The apoptosis-inducing agent *Pleurotus ostreatus* water-soluble protein mixing device according to claim 5, characterized in that, A support block (59) is fixedly installed on the surface of the rotating shaft (2), and a threaded groove (510) extending into the support block (59) is opened on the surface of the mounting bracket (4), and a locking bolt (511) is installed inside the threaded groove (510).

7. The apoptosis-inducing agent *Pleurotus ostreatus* water-soluble protein mixing device according to claim 6, characterized in that, The mating surface of one side of the semicircular plate (55) is provided with a number of slots (6) at equal intervals along its length, and the mating surface of the other side of the semicircular plate (55) is provided with a number of locking blocks (61) that are compatible with the slots (6).

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