Microfluid box for rapid detection of biomolecules

Abstract

The invention relates to the technical field of biological detection, and particularly discloses a microfluid box for rapid detection of biomolecules, the microfluid box comprises a box body, a fluid channel, a porous membrane, a flow driving structure, a mixed guide structure and a detection cavity. The fluid channel is communicated with the liquid inlet, and the porous membrane is arranged in the channel and used for sample cracking and target object capturing; the driving structure comprises a liquid storage bin and a driving assembly, can pre-store and sequentially release various reagents, and generates negative pressure and positive pressure through manual operation to drive fluid; the mixing guide structure is provided with a spiral flow guide part to realize efficient passive mixing of reagents; the detection cavity is provided with an optical window and a probe array for signal generation and reading. According to the invention, the whole processes of sample treatment, reagent release, fluid driving, mixing and detection are highly integrated in the kit body, the integrated rapid detection of sample inlet and result outlet is realized, and the kit has the advantages of simplicity and convenience in operation, strong portability, good pollution resistance and accurate and reliable detection result.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological detection, in particular to a microfluidic cartridge for rapid detection of biomolecules. BACKGROUND

[0002] Traditional biomolecule detection methods usually involve multiple independent steps such as sample pretreatment, reagent addition, mixing reaction, and result reading, which are cumbersome to operate and highly dependent on professional personnel and laboratory environment, making it difficult to meet the demand for on-site rapid detection.

[0003] At present, Chinese patent application No. CN202310305686.4 discloses a microfluidic biological sample detection chip integrating pumping and micro-mixing functions. The invention aims to solve the problem of long mixing time in microfluidic chips. It uses a piezoelectric driving method to realize the integration of liquid pumping and micro-mixing functions in the same pump cavity or the same structural unit, so that biological reagents can be pumped quantitatively from the inlet to the sample detection channel, and the reagents can be fully mixed with fluorescent microspheres or magnetic beads, achieving efficient integration of structure and function.

[0004] However, the existing technology relies on external precision electronic components such as piezoelectric drivers during the detection of biomolecules, which not only increases the manufacturing cost and system complexity of the chip, but also makes it difficult for the entire detection device to completely eliminate external power supply and control circuit, affecting its applicability and portability in environments without stable power supply or limited resources. Secondly, such active driving methods have high requirements for the precision and reliability of components, which can easily increase the failure rate. SUMMARY

[0005] The present application aims to provide a microfluidic cartridge for rapid detection of biomolecules to solve the problems raised in the background art.

[0006] To achieve the above-mentioned purpose, the present application adopts the following technical solution: a microfluidic cartridge for rapid detection of biomolecules, comprising a cartridge body, a sheath, a liquid inlet, a sealing plug, a fluid channel, a porous membrane, a flow driving structure, a mixing guide structure, a detection cavity, and an exhaust channel. The cartridge body is wrapped with a sheath, and a liquid inlet is formed in the upper middle part of the front of the cartridge body. A sealing plug is arranged inside the liquid inlet. A fluid channel is arranged in the upper middle part of the inside of the cartridge body, and the top side of the fluid channel is connected to the liquid inlet. A porous membrane is arranged in the lower middle part of the inside of the fluid channel, and a flow driving structure is connected to the right side below the porous membrane. The bottom of the flow driving structure is connected to a mixing guide structure, and the bottom of the mixing guide structure is inserted into the inside of a detection cavity. The detection cavity is arranged in the lower middle part of the inside of the cartridge body. An exhaust channel is arranged in the upper rear part of the detection cavity.

[0007] The driving structure comprises a liquid inlet pipe connected with the fluid channel on the left side, a liquid storage bin fixed on the top of the liquid inlet pipe away from the fluid channel, and at least one partition plate embedded in the liquid storage bin to form multiple storage chambers, and a liquid storage bubble embedded in the storage chamber.

[0008] Preferably, the porous membrane separates the fluid channel into two parts, and the surface or interior of the porous membrane is pre-embedded with a lysis reagent or a nucleic acid binding solid phase material, which can be lysed and achieve the capture and purification of target molecules when the sample liquid flows through the porous membrane.

[0009] Preferably, the bottom of the detection chamber is an optically transparent detection window, and the top inner wall of the detection chamber is fixed with a pre-embedded detection probe array, which is opposite to the detection window, so as to facilitate reading the detection signal from the bottom of the box by optical equipment (such as a fluorescence scanner).

[0010] Preferably, the bottom of the detection chamber is an optically transparent detection window, and the top inner wall of the detection chamber is fixed with a pre-embedded detection probe array, which is opposite to the detection window, so as to facilitate reading the detection signal from the bottom of the box by optical equipment (such as a fluorescence scanner).

[0011] Preferably, the top side of the liquid inlet pipe is provided with the same number of openings corresponding to the storage chambers, and the inner middle and lower sides of the openings are embedded with hollow pieces to connect the storage chambers and the liquid inlet pipe, and the hollow pieces are integrally formed with sharp heads above them, and the top tips of the sharp heads are inserted into the inner bottom side of the storage chamber.

[0012] Preferably, the driving assembly comprises a silo fixed in the box, two hollow columns are arranged on the upper and lower sides of the silo, and the hollow columns on the upper and lower sides are arranged in pairs, and a ring-shaped piece is fixed above the two hollow columns on the upper side and below the two hollow columns on the lower side, a hinge piece is hinged to the right bottom of the ring-shaped piece, and the hinge piece is in contact with the ring-shaped piece to form a seal when the hinge piece is rotated to the top side, a sliding rod penetrates and slides in the right side of the silo, and a sliding plug is connected to the left end of the sliding rod, the sliding plug is slidingly connected in the silo, the sliding plug is abutted by a spring, one end of the spring away from the sliding plug is connected with the silo, the two hollow columns on the upper side are connected with the liquid inlet pipe, and the two hollow columns on the lower side are connected with the liquid outlet pipe.

[0013] Preferably, the silo right side is embedded in the right side wall of the box body, and a ring-shaped internal thread groove is arranged on the inner side of the right part of the silo, one end of the sliding rod away from the sliding block is rotationally connected with a rotating disc, an external thread convex ring is arranged on the outer periphery of the side of the rotating disc close to the silo, and when the sliding rod moves to the leftmost side, the external thread convex ring is used for being screwed into the internal thread groove to lock the position of the sliding rod.

[0014] Preferably, the mixed guide structure comprises three micro flow channels, the bottoms of the three micro flow channels are connected with a liquid collecting seat, a spiral flow guide part is longitudinally arranged on the left side of the inside of the liquid collecting seat, and the rear upper part of the spiral flow guide part is connected with a guide micro tube, and the other end of the guide micro tube is inserted into the inside of a detection cavity, so that the liquids from different flow paths can be fully mixed in the spiral flow guide part and then smoothly guided into the detection cavity.

[0015] Compared with the prior art, the present application has the following beneficial effects:

[0016] The present application highly integrates the complete process of sample lysis and purification, multi-reagent pre-storage and sequential release, fluid driving, mixing and final detection into one box body, and the user only needs to perform simple operations such as sample adding, pressing and pulling to automatically complete the whole process from sample processing to result output, so that the integrated design of sample input and result output greatly reduces the professional requirements for the operator, shortens the detection time, and can maximize the avoidance of sample cross contamination, reagent contamination and aerosol contamination caused by manual operation, and significantly improves the reliability and safety of detection.

[0017] The present application optimally sets the flow driving structure and the mixed guide structure, realizes the fluid control without an external complex pump valve system, integrates the one-way valve and the mechanical locking mechanism into the manually operated driving assembly, can reliably generate negative pressure and positive pressure, accurately controls the liquid suction, pumping and process pause, and the power source is simple and reliable; at the same time, the spiral flow guide part uses the passive micro flow principle to make the multi-path reagents realize rapid and sufficient mixing during the conveying process, ensures the reaction efficiency and uniformity, makes the whole detection system not rely on large peripheral equipment, has compact structure, is suitable for on-site rapid detection and use in resource-limited environment, and enhances the portability and scene adaptability of the product.

[0018] The porous membrane module of the present application realizes the instant online lysis and purification of the sample, provides high-quality targets for the subsequent reaction, ensures the stability of the long-term storage of the reagent and the accurate controllability of the release through the independent liquid storage bubble cap and the sharp head piercing structure, eliminates the bubble interference through the detection cavity cooperating with the exhaust channel and the optical window, and optimizes the signal acquisition path, so that the stable and efficient microfluidic working system is formed in the box body, and the high sensitivity of the detection process and the reliability of the result are ensured as a whole. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 Structure diagram of the present application;

[0020] Figure 2 Structure diagram of the present application;

[0021] Figure 3 Structure diagram of the present application;

[0022] Figure 4 Structure diagram of the present application;

[0023] Figure 5 Structure diagram of the present application Figure 4 Structure diagram of the present application

[0024] Figure 6 Structure diagram of the present application;

[0025] Figure 7 Structure diagram of the present application;

[0026] In the figure: box body-1, sheath-2, liquid inlet-3, sealing plug-4, fluid channel-5, porous membrane-6, flow driving structure-7, mixed guiding structure-8, detection cavity-9, exhaust channel-10, liquid inlet pipe-71, liquid storage warehouse-72, partition-73, liquid storage bubble-74, driving assembly-75, liquid outlet pipe-76, through hole-711, hollow sheet-712, sharp head-713, silo-751, annular sheet-752, opening and closing sheet-753, sliding rod-754, sliding plug-755, spring-756, annular internal thread groove-7511, rotating disc-7541, external thread convex ring-7542, microfluidic channel-81, liquid collection seat-82, spiral flow guide-83, guide microtube-84. DETAILED DESCRIPTION

[0027] In order to further explain the technical solutions of the present application, the following specific embodiments are described in detail.

[0028] Please refer to Figure 1 and Figure 2 The present application provides a microfluidic box for rapid detection of biomolecules, which comprises a flat box body 1, a sheath 2, a liquid inlet 3, a sealing plug 4, a fluid channel 5, a porous membrane 6, a flow driving structure 7, a mixed guiding structure 8, a detection cavity 9 and an exhaust channel 10.

[0029] The box 1 is injection molded by transparent polymer material, facilitating observation of internal liquid flow and reaction state; and the box 1 is wrapped with a sheath 2, providing anti-slip holding feeling, and physically protecting and beautifying the internal structure; a liquid inlet 3 is formed in the upper middle of the front of the box 1, and a sealing plug 4 is arranged inside the liquid inlet 3, ensuring that the liquid inlet 3 is reliably sealed after adding the sample, preventing evaporation, pollution or leakage of biological hazards;

[0030] A fluid channel 5 is arranged in the upper middle of the inside of the box 1, and the top side of the fluid channel 5 is connected with the liquid inlet 3, providing an initial flow path for the sample; a porous membrane 6 is arranged in the lower middle of the inside of the fluid channel 5, and the porous membrane 6 divides the fluid channel 5 into two parts, constituting a physical filtration and reaction interface; a lysing reagent or nucleic acid binding solid phase material is pre-embedded on the surface or inside of the porous membrane 6, which can instantly lyse cells / viruses and specifically capture the released target nucleic acid when the sample liquid flows through the porous membrane 6, realizing on-site rapid purification of the sample and saving the cumbersome step of centrifugation;

[0031] A driving structure 7 is arranged on the right side below the porous membrane 6, and the driving structure 7 is connected with the fluid channel 5, providing fluid power and reagent source for the subsequent process; a mixing and guiding structure 8 is connected to the bottom of the driving structure 7, and the bottom of the mixing and guiding structure 8 is inserted into a detection cavity 9, to constitute a continuous flow path from driving, mixing to detection; the detection cavity 9 is arranged in the lower middle of the inside of the box 1, and the bottom thereof is an optically transparent detection window, allowing an external optical detection device to read signals from below without interference, and the top inner wall of the detection cavity 9 is fixed with a pre-embedded detection probe array (such as a microarray fixed with specific oligonucleotide probes or antigens / antibodies), and the detection probe array is opposite to the detection window, ensuring that the signals generated by the reaction can be efficiently collected, improving the sensitivity of detection; an exhaust channel 10 is arranged in the upper rear part of the detection cavity 9, to exhaust the internal air when the liquid is injected into the detection cavity 9, ensuring that the cavity is completely and bubble-free filled, guaranteeing reaction uniformity and detection accuracy.

[0032] Please refer to Figures 1-6The application provides a microfluidic box for rapid detection of biomolecules, and a driving flow structure 7 comprises a liquid inlet pipe 71 connected with the fluid channel 5 on the left side, a liquid storage bin 72 fixed and locked on the top side of the liquid inlet pipe 71 away from the fluid channel 5, and not less than one partition plate 73 embedded in the liquid storage bin 72 to form multiple independent storage chambers in the liquid storage bin 72, so that multiple reagents are physically stored separately to avoid mutual contamination or early reaction; a liquid storage bubble 74 is arranged in each storage chamber, and a cushion is arranged on the left and right sides of the bottom of the storage chamber to support the liquid storage bubble 74 and protect the liquid storage bubble 74 from being accidentally broken during transportation and storage, so as to ensure the stability of the reagent; different liquid reagents such as elution buffer, nucleic acid amplification reaction premix (such as LAMP and RPA reagent), and fluorescence detection substrate are stored in the multiple liquid storage bubbles 74, so that all key reagents required in the detection process are integrated in the box to realize reagent prestorage, and users do not need to prepare or manually add extra reagents, so that the operation is simplified and the pollution risk is reduced.

[0033] The top side of the liquid inlet pipe 71 is provided with the same number of through holes 711 corresponding to the storage chambers, and hollow pieces 712 are embedded in the inside of the through holes 711, which are isolated from the storage chambers in the normal state and become liquid channels when needed; the hollow pieces 712 are integrally formed with sharp heads 713 above, and the top tips of the sharp heads 713 are inserted into the inside bottom side of the storage chamber to form a reliable piercing structure; when a user presses the area above the corresponding storage chamber from the outside of the box body 1, the liquid storage bubble 74 is pushed downward and pierced by the sharp head 713, forming a simple, reliable and user-initiated reagent release mechanism, which can activate different reagents in sequence according to the detection process, and the released liquid reagent immediately flows into the liquid inlet pipe 71 through the hollow piece 712.

[0034] The two outlets of the bottom of the liquid inlet pipe 71 are connected with a driving assembly 75, and the driving assembly 75 comprises a silo 751 fixed in the inside of the box body 1, two hollow columns are arranged on the upper and lower sides of the silo 751, and the two hollow columns on the upper and lower sides are arranged in pairs, the upper two hollow columns are fixed above, and the lower two hollow columns are fixed below, and the bottom right side of the annular piece 752 is hinged with an opening and closing piece 753, which together constitute a one-way valve structure, and when the opening and closing piece 753 is rotated to the top side, it is in contact with the annular piece 752 to form a seal, so that the fluid can only pass in one direction to prevent backflow.

[0035] The slide rod 754 is slidably arranged in the silo 751, and a slide plug 755 is connected to the left end of the slide rod 754 and is slidably connected in the silo 751, the slide plug 755 is abutted by a spring 756, one end of the spring 756 away from the slide plug 755 is connected with the silo 751, the spring 756 provides a reset elastic force for the slide plug 755, and the pulling and pushing operation of the user is converted into the suction and pushing force of the liquid; the upper two hollow columns are connected with the liquid inlet pipe 71, and the lower two hollow columns are connected with the liquid outlet pipe 76, and a complete manual pumping circuit is formed.

[0036] The silo 751 is embedded in the right side wall of the box body 1, and an annular internal thread groove 7511 is formed in the inner side of the right part of the silo 751, a rotating disc 7541 is rotatably connected to one end of the slide rod 754 away from the slide plug 755, an external thread convex ring 7542 is arranged on the side of the rotating disc 7541 close to the silo 751, and a separable locking mechanism is formed, after the user pulls the rotating disc 7541 outward to make the slide rod 754 drive the slide plug 755 to move rightward (suction stage), the rotating disc 7541 is pushed back to the left side and rotated, so that the external thread convex ring 7542 is screwed and locked into the annular internal thread groove 7511, and the slide plug 755 is locked at the compression position, and the user can perform other operations (such as pressing the next liquid storage bubble cover) in the locked state.

[0037] The two outlets of the driving assembly 75 are connected with the liquid outlet pipe 76, the liquid outlet pipe 76 is provided with three flow outlets at the bottom, and the three flow outlets are connected with the mixed guide structure 8, so that the pumped liquid is reasonably distributed into the mixed guide structure 8.

[0038] Please refer to Figure 2 、 Figure 3 and Figure 7 , the present application provides a kind of microfluidic box for rapid detection of biomolecule, mixed guide structure 8 includes three microfluidic channels 81, three microfluidic channels 81 bottom are connected with liquid collection seat 82, and the liquid flow from different outlets of liquid outlet pipe 76 is preliminarily gathered;Spiral flow guide part 83 is arranged in the left side of the inside of liquid collection seat 82 longitudinally, the mixing path is extended by using spiral flow channel and micro eddy current is generated, so that multi-component liquid (such as eluent, amplification reagent, detection reagent) pumped into or from different flow paths in sequence realizes rapid, sufficient passive mixing here, without external stirring device;

[0039] The rear upper part of spiral flow guide part 83 is connected with guide microtube 84, and the other end of guide microtube 84 is inserted into the inside of detection chamber 9, so that the reaction liquid after sufficient mixing is smoothly and controllably guided into the specific position of detection chamber 9, direct liquid impact on detection probe array is avoided, and the uniform and stable reaction interface is guaranteed.

[0040] The application provides a microfluidic box for rapid detection of biomolecules.

[0041] First, the user opens the sealing plug 4, and adds a liquid sample to be detected through the liquid inlet 3. The sample enters the fluid channel 5 under the action of gravity or subsequent negative pressure, and flows through the porous membrane 6 in which the lysis reagent and the nucleic acid binding solid phase material are embedded. The cells or viruses in the sample are instantaneously lysed, and impurities are separated, so that the rapid lysis and purification of the sample are automatically completed in the box, and clean target materials are provided for subsequent detection.

[0042] Second, according to the detection process sequence, the corresponding storage chamber area of the liquid storage bin 72 is pressed from the outside of the box body 1. The pressing action makes the liquid storage bubble 74 in the storage chamber move downward, and the liquid reagent (such as elution buffer, amplification reaction liquid, detection substrate, etc.) sealed in different liquid storage bubble 74 is thus released on demand and in sequence, and flows into the liquid inlet pipe 71 below through the hollow sheet 712.

[0043] Third, the liquid is driven to flow along a preset path by operating the driving assembly 75. Specifically, the user pulls the rotating disc 7541 outward, drives the sliding rod 754 and the sliding plug 755 to move rightward, and generates negative pressure in the left chamber of the silo 751. The liquid in the liquid inlet pipe 71 is sucked into the silo 751 through the one-way valve composed of the annular sheet 752 and the opening and closing sheet 753. Then, the user pushes the rotating disc 7541 back and rotates, so that the external thread convex ring 7542 is locked into the annular internal thread groove 7511. At this time, the sliding plug 755 moves leftward to generate positive pressure, and the liquid in the cavity is pumped out to the liquid outlet pipe 76 through the lower one-way valve, and is delivered to the mixed guide structure 8. After the liquid is preliminarily distributed in the three microfluidic channels 81 of the mixed guide structure 8, it is collected into the spiral guide part 83 of the liquid collection seat 82. The micro eddy current generated by the spiral flow channel realizes rapid and sufficient passive mixing of different component liquids. Finally, the mixed liquid is smoothly guided into the detection cavity 9 through the guide micro tube 84.

[0044] Fourth, after the mixed reaction liquid enters the detection cavity 9, the air in the cavity is discharged through the exhaust channel 10, so as to ensure that the liquid completely fills the cavity. The target molecules in the liquid react with the detection probe array pre-embedded on the top wall of the detection cavity 9 (such as hybridization or immune combination), and generate a detectable signal (such as fluorescence). At this time, through the detection window at the bottom of the box body 1, the external optical detection equipment can collect and read the reaction signal without interference, so as to complete the rapid detection process of the sample in and the result out.

[0045] The above merely describes the preferred examples of the present application and is not intended to limit the present application. Although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art will appreciate that modifications can be made to the foregoing embodiments, or some of the technical features thereof can be equivalently replaced, without departing from the spirit and principle of the present application. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A microfluidic cartridge for rapid detection of biomolecules, comprising a cartridge body (1), wherein a sheath (2) is provided on the outer surface of the cartridge body (1), and a liquid inlet (3) is provided on the upper front side of the cartridge body (1), wherein a sealing plug (4) is provided inside the liquid inlet (3), and a fluid channel (5) is provided in the upper middle part of the cartridge body (1), wherein the top side of the fluid channel (5) is connected to the liquid inlet (3), characterized in that, A porous membrane (6) is provided on the lower side of the fluid channel (5), and a flow-driving structure (7) is provided on the right side below the porous membrane (6). A mixing and guiding structure (8) is connected to the bottom of the flow-driving structure (7), and the bottom of the mixing and guiding structure (8) is inserted into the detection chamber (9). The detection chamber (9) is located in the lower middle part of the box body (1), and an exhaust channel (10) is provided at the upper rear part of the detection chamber (9). The flow-driving structure (7) includes an inlet pipe (71) connected to the fluid channel (5) on the left side, and the top of the inlet pipe (71) is far away from the fluid channel (5). A liquid storage tank (72) is locked and fixed on one side of the fluid channel (5). At least one partition (73) is embedded inside the liquid storage tank (72) to form multiple storage chambers inside the liquid storage tank (72). A liquid storage bubble (74) is provided in the storage chamber. The two outlets at the bottom of the liquid inlet pipe (71) are connected to the drive assembly (75). The two outlets at the bottom of the drive assembly (75) are connected to the liquid outlet pipe (76). The bottom of the liquid outlet pipe (76) is provided with three flow outlets, and the bottom of the three flow outlets is connected to the mixing and guiding structure (8).

2. The microfluidic cartridge for rapid detection of biomolecules according to claim 1, characterized in that: The porous membrane (6) divides the fluid channel (5) into upper and lower parts, and the surface or interior of the porous membrane (6) is pre-embedded with lysis reagents or nucleic acid binding solid phase materials.

3. The microfluidic cartridge for rapid detection of biomolecules according to claim 1, characterized in that: The bottom of the detection cavity (9) is an optically transparent detection window, and a pre-embedded detection probe array is fixed on the top inner wall of the detection cavity (9), with the detection probe array facing the detection window.

4. The microfluidic cartridge for rapid detection of biomolecules according to claim 1, characterized in that: The storage chamber has pads on both the left and right sides at the bottom to support the liquid storage blister (74), and different liquid reagents are sealed inside the multiple liquid storage blister (74).

5. The microfluidic cartridge for rapid detection of biomolecules according to claim 1, characterized in that: The top side of the inlet pipe (71) is provided with the same number of openings (711) as the storage chamber, and a hollow piece (712) is embedded in the lower middle side of the opening (711) to connect the storage chamber and the inlet pipe (71).

6. The microfluidic cartridge for rapid detection of biomolecules according to claim 5, characterized in that: The hollow piece (712) has a pointed tip (713) integrally formed on its upper part, and the top tip of the pointed tip (713) is inserted into the bottom side of the storage chamber.

7. The microfluidic cartridge for rapid detection of biomolecules according to claim 1, characterized in that: The drive assembly (75) includes a silo (751) fixed inside the housing (1). Two hollow columns are provided on both the upper and lower sides of the silo (751), and the hollow columns on the upper and lower sides are arranged in pairs. Annular plates (752) are fixed above the two upper hollow columns and below the two lower hollow columns. An opening / closing plate (753) is hinged to the bottom right side of the annular plate (752). When the opening / closing plate (753) rotates to its highest position, it contacts the annular plate (752) to form a seal. A sliding rod (754) slides through the right side of the interior, and a sliding plug (755) is connected to the left end of the sliding rod (754). The sliding plug (755) is slidably connected to the inside of the silo (751). The sliding plug (755) abuts against a spring (756). The end of the spring (756) away from the sliding plug (755) is connected to the silo (751). The two hollow columns on the upper side are connected to the inlet pipe (71) above, and the two hollow columns on the lower side are connected to the outlet pipe (76) below.

8. A microfluidic cartridge for rapid detection of biomolecules according to claim 7, characterized in that: The silo (751) is embedded in the right side wall of the box body (1), and an annular internal thread groove (7511) is provided on the inner side of the right side of the silo (751).

9. A microfluidic cartridge for rapid detection of biomolecules according to claim 8, characterized in that: The end of the slide rod (754) away from the sliding block (755) is rotatably connected to a turntable (7541). The turntable (7541) has an external threaded protrusion ring (7542) on its outer periphery near the silo (751). When the slide rod (754) moves to the leftmost position, the external threaded protrusion ring (7542) is used to spirally lock into the annular internal thread groove (7511).

10. A microfluidic cartridge for rapid detection of biomolecules according to claim 1, characterized in that: The mixed-conducting structure (8) includes three microchannels (81), the bottom of which is connected to the liquid collection seat (82). The liquid collection seat (82) has a spiral guide section (83) arranged longitudinally on the left side, and the upper rear part of the spiral guide section (83) is connected to the guide microtube (84). The other end of the guide microtube (84) is inserted into the detection cavity (9).

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